CN112042643B - Insect food attractant and preparation method and application thereof - Google Patents
Insect food attractant and preparation method and application thereof Download PDFInfo
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- CN112042643B CN112042643B CN202010937549.9A CN202010937549A CN112042643B CN 112042643 B CN112042643 B CN 112042643B CN 202010937549 A CN202010937549 A CN 202010937549A CN 112042643 B CN112042643 B CN 112042643B
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- insect
- attractant
- food attractant
- insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
Abstract
The invention provides an insect food attractant and a preparation method and application thereof, the insect food attractant comprises arborvitae, and the preparation method comprises the following steps: the thujene is prepared into a solution with the concentration of 50v/v% -57 v/v% to obtain the insect feeding attractant. The insect food attractant can trap bemisia tabaci, aphids and thrips simultaneously, can reduce the incidence rate of vegetable virus diseases, is safe and pollution-free, and is a new technology for preventing and controlling the vegetable virus diseases at present. The preparation method is simple, can be applied to trapping of field insects, and has the advantages of high trapping efficiency, capability of preventing and controlling the generation of vegetable viruses and the like.
Description
Technical Field
The invention relates to the technical field of pest control, in particular to an insect food attractant and a preparation method and application thereof.
Background
Vegetable virus diseases are a kind of diseases which seriously harm vegetable production and seriously affect the quality and safety of vegetable production. Most of vegetable virus diseases are transmitted by vector insects, such as bemisia tabaci, aphids, thrips and the like, the conventional prevention and control measures mainly rely on chemical pesticides, but the abuse or random use of pesticides in production causes the development of pest resistance, and the overproof pesticide residues and environmental pollution of vegetable products.
In the field, bemisia tabaci, aphids and thrips are mixed, but the bemisia tabaci, the aphids and the thrips are three different mediator insects, have obvious differences in aspects of growth, development, feeding and the like, and have obvious differences in plant selectivity. Currently, only one insect can be induced and collected in the field, and an induced and collected material which has the effect on three pests is screened, and the induced and collected effect on the pests and the prevention and control effect on virus diseases are necessary to be researched and developed.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides the insect food attractant capable of trapping bemisia tabaci, aphids and thrips simultaneously, the preparation method is simple, the incidence rate of vegetable virus diseases can be reduced simultaneously, and the insect food attractant is safe and pollution-free and is a novel technology for preventing and controlling the vegetable virus diseases at present.
In order to solve the technical problem, the invention provides an insect feeding attractant which comprises thujene.
The insect feeding attractant further comprises the cedrene with the concentration of 50v/v% -57 v/v%.
The above insect food attractant further comprises: 0.1 to 0.2v/v percent of 2, 4-dihydroxy benzophenone, 0.2 to 0.4v/v percent of 2-hydroxy-4-n-octoxy benzophenone and 0.2 to 0.4v/v percent of 6-tert-butyl-p-cresol.
The insect feeding attractant further comprises 3-5 v/v% of alcohol, 3-5 v/v% of cane sugar, 18-22 v/v% of glucose and 18-20 v/v% of ethyl acetate.
Based on a general technical concept, the invention also provides a preparation method of the insect food attractant, which comprises the following steps:
the thujene is prepared into a solution with the concentration of 50v/v% -57 v/v% to obtain the insect feeding attractant.
The preparation method further comprises mixing the thujaplicin, the alcohol, 50% of sucrose, 50% of glucose and ethyl acetate at a volume ratio of 50-57: 3-5: 18-22: 18-20 to obtain the insect phagostimulant.
The method for producing the attractant for insect feeding comprises mixing together arborvitae, ethanol, 50% sucrose, 50% glucose, ethyl acetate, 2, 4-dihydroxybenzophenone, 2-hydroxy-4-n-octoxybenzophenone, and 6-tert-butyl-p-cresol at a volume ratio of 50 to 57: 3 to 5: 18 to 22: 18 to 20: 0.1 to 0.2: 0.2 to 0.4.
Based on a general technical concept, the invention also provides an application of the insect food attractant in insects.
The above use, further, the insect comprises: trapping bemisia tabaci, aphids and thrips.
The above application, further, the method of the application is: the method for trapping insects by using the insect food attractant comprises the following steps: the insect attractant is placed at an attracting core of the insect trap, 20-50 ml of 10wt% cane sugar water is filled at the bottom of the insect trap, the insect attractant is added every 14 days, and meanwhile, the cane sugar water in a container is replaced.
Compared with the prior art, the invention has the advantages that:
(1) the invention provides an insect food attractant, wherein the main component of the arborvitae can simultaneously attract and collect field mediators of tobacco whitefly, aphid and thrips, and simultaneously reduce the incidence rate of vegetable virus diseases, is safe and pollution-free, and is a new technology for preventing and controlling the vegetable virus diseases at present.
(2) The invention provides an insect feeding attractant, wherein the mixture of 2, 4-dihydroxy benzophenone, 2-hydroxy-4-n-octoxy benzophenone and 6-tert-butyl-p-cresol can absorb ultraviolet rays in sunlight and improve the stability of the insect feeding attractant.
(3) The invention provides an insect feeding attractant, wherein the attraction effect can be increased by mixing alcohol, 50wt% of cane sugar, 50wt% of glucose, ethyl acetate and the like.
(4) The invention provides a preparation method of an insect phagostimulant, which has simple and controllable preparation process and can be used for industrial production.
(5) The invention provides an application of an insect phagostimulant in trapping insects, and combines an indoor Y-shaped tube test and a field test to determine that the material simultaneously has an attraction effect on mediator insects such as bemisia tabaci, aphids, thrips and the like, and research and develop a prevention and control technology for vegetable virus diseases, so that the occurrence of the vegetable virus diseases is reduced in the field.
(6) The invention provides an application of an insect phagostimulant in trapping insects, wherein the insect phagostimulant is placed at a lure core of an insect trap, so that insects can be attracted into the trap and can be collected, cane sugar water at the bottom of the insects entering the trap can attract the insects to eat so as to kill the insects and prevent the insects from escaping, a cover at the top end can be waterproof, and the effect can be exerted in rainy days.
Detailed Description
The invention is further described below with reference to specific preferred embodiments, without thereby limiting the scope of protection of the invention.
The materials and equipment used in the following examples are commercially available.
Example 1:
an insect feeding attractant comprises thujene.
The preparation method of the insect food attractant comprises the following steps:
arborvitae was dissolved in alcohol to prepare a 50wt% solution.
Example 2:
an insect feeding attractant comprises 50v/v% of arborvitae, 5v/v% of alcohol, 5v/v% of 50wt% of cane sugar, 20v/v% of 50wt% of glucose and 20v/v% of ethyl acetate.
The preparation method of the insect food attractant comprises the following steps:
(1) 50g of sucrose was dissolved in 100mL of water to give a 50wt% sucrose solution, and 50g of glucose was dissolved in 100mL of water to give a 50wt% glucose solution.
(2) Mixing the arborvitae, alcohol, 5wt% of sucrose, 50wt% of glucose and ethyl acetate at a volume ratio of 50: 5: 20 to obtain the insect food attractant.
Example 3:
an insect feeding attractant comprises 50v/v% of arborvitae, 5v/v% of alcohol, 5v/v% of 50wt% of cane sugar, 20v/v% of 50wt% of glucose, 19v/v% of ethyl acetate, 0.2v/v% of 2, 4-dihydroxybenzophenone, 0.4v/v% of 2-hydroxy-4-n-octoxybenzophenone and 0.4v/v% of 6-tert-butyl-p-cresol.
The preparation method of the insect food attractant comprises the following steps:
(1) 50g of sucrose was dissolved in 100mL of water to give a 50wt% sucrose solution, and 50g of glucose was dissolved in 100mL of water to give a 50wt% glucose solution.
(2) Mixing arborvitae, alcohol, 50wt% of sucrose, 50wt% of glucose, ethyl acetate, 2, 4-dihydroxybenzophenone, 2-hydroxy-4-n-octyloxybenzophenone and 6-tert-butyl-p-cresol at a volume ratio of 50: 5: 20: 0.2: 0.4 to obtain the insect phagostimulant.
Example 4:
the application of the insect food attractant of the embodiment 1 and the embodiment 2 in trapping bemisia tabaci, aphids and thrips simultaneously comprises the following application methods:
in the Y-shaped tube test, one end of an odor source bottle containing the insect food attractant in example 1 or example 2 is connected with an air compressor after activated carbon purification, the other end is connected with one arm of a Y-shaped tube, and the other arm of the Y-shaped tube is connected with purified air for comparison. Under the action of an oil-free air compressor, two purified air flows enter the odor source bottle at the speed of 100 mL/min and then enter the two arms of the Y-shaped pipe. The starvation-treated insects were introduced into the ends of the Y-stems for 8 h each time. Each insect was observed for a maximum of 20 min. Selection was recorded when it entered an arm beyond 3cm and "no selection" was recorded when it remained inactive for 20 min. The Y-tube was replaced every 10 tests and the position of the two odor source bottles was changed to eliminate potential asymmetric effects. After completion of the test, the used Y-tube was washed with 75% ethanol and dried in an oven at 65 ℃. 30 insects were observed at a time, and 9 replicates were analyzed to examine the tropism of different mediator insects to the insect phagostimulants of examples 1 and 2.
Table 1: indoor Y-shaped pipe test for researching trapping effect of arborvitae and compound material thereof on different mediator insects
From the results in table 1, the trapping effect of the arborvitae on bemisia tabaci, aphids and thrips is higher than 60%, and then the trapping effect of the mixture on different vectors of insects is researched by adding the mixture of alcohol, cane sugar, glucose and ethyl acetate, and the food attractant formula shows high-efficiency trapping effect after 9 repeated indoor tests.
Example 5:
the application of the insect food attractant in the step of trapping bemisia tabaci, aphids and thrips simultaneously in the embodiment 3 comprises the following steps:
when the number of the mediator insects on each plant in the field exceeds 10, an insect polyethylene slow-release bottle (also called fruit fly trapper and purchased from green field and field), 20-50 ml of 10% sucrose water is filled in the bottle bottom, the insect food attractant in the embodiment 3 is dripped on a filter paper attracting core, and the mediator insects in the field are trapped and killed. Adding a phagostimulant liquid material every 14 days, simultaneously pouring out the sucrose water and the vector insects in the polyethylene bottle, and replacing the sucrose water.
The indoor method was the same as in example 4.
In the insect polyethylene slow-release bottle in the embodiment, the bottle body is yellow, the height is 20cm, the cover is blue, the diameter of the bottle cover is 10cm, 4 round holes with the diameter of 3cm are formed in the periphery of the bottle cover, a threaded connecting port with the diameter of 2mm is arranged in the middle of the bottle cover and right opposite to the bottle bottom, a hanging hook with the diameter of 2mm and the length of 5cm can be hung, and the bottom of the hanging hook is designed to be a closed circular ring shape. The multi-layer filter paper is made into a lure core with the diameter of 3cm, the diameter of a middle opening is 2mm, the middle of the lure core can just penetrate through the polyethylene round hook and is clamped at the bottom of the round hook, and the top of the round hook is screwed on the threaded connecting port. The periphery of the bottle cap is provided with three plastic buckles which form a triangle, the other end of the bottle cap is connected with a cover, and the cover is yellow to prevent the food attractant material from being drenched by rain.
The indoor and outdoor methods were analyzed in 9 replicates.
After 7 days the different vectors were examined for their tropism towards the insect phagostimulant of example 3.
Table 2: trapping effect of indoor and field food attractant material
As can be seen from the results in table 2, the insect food attractant formulation of example 3 exhibited highly effective trapping both indoors and in the field.
Example 6:
the application of the insect phagostimulant of example 2 and the insect phagostimulant of example 3 to the simultaneous trapping of bemisia tabaci, aphids and thrips was examined in the same manner as in example 5, and after 14 days the tropism of different mediator insects to the insect phagostimulants of example 2 and example 3, respectively, was examined.
Table 3: increasing trapping effect of light stabilizer mixture in field
From the results in table 3, it can be seen that: in example 2, no light stabilizer such as 2, 4-dihydroxybenzophenone, 2-hydroxy-4-n-octoxybenzophenone, and 6-tert-butyl-p-cresol was added, and the insect-trapping effect was significantly lower than that of example 3.
Example 7:
the application of the insect food attractant in the embodiment 3 in reducing the incidence rate of vegetable diseases comprises the following steps:
when the number of the mediator insects on each plant in the field exceeds 10, a polyethylene bottle is placed, 20-50 ml of 10% cane sugar water is filled into the bottom of the polyethylene bottle, the food attractant liquid material is dripped onto the filter paper attracting core, and the mediator insects in the field are attracted and killed. Adding a phagostimulant liquid material every 14 days, simultaneously pouring out the sucrose water and the vector insects in the polyethylene bottle, and replacing the sucrose water. The field virus incidence was examined after 30 days.
Table 4: incidence of viral disease using food attractant formulation and control field
From the investigation results in table 3, it can be seen that: the occurrence of virus diseases is investigated, and the insect food attractant reduces the occurrence of vegetable virus diseases after being used, and compared with a control without the food attractant, the insect food attractant can obviously reduce the occurrence of virus diseases such as Tomato Yellow Leaf Curl Virus (TYLCV), tomato chlorosis virus (ToCV), Cucumber Mosaic Virus (CMV), Tomato Spotted Wilt Virus (TSWV) and the like.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner. Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. Those skilled in the art can make many possible variations and modifications to the disclosed embodiments, or equivalent modifications, without departing from the spirit and scope of the invention, using the methods and techniques disclosed above. Therefore, any simple modification, equivalent replacement, equivalent change and modification made to the above embodiments according to the technical essence of the present invention are still within the scope of the protection of the technical solution of the present invention.
Claims (7)
1. The application of the cedrene in preparing the insect feeding attractant for trapping insects is characterized in that in the insect feeding attractant, the concentration of the cedrene is 50v/v% -57 v/v%, and the insects are aphids.
2. The use of claim 1, wherein said insect phagostimulant further comprises 3 to 5v/v% alcohol, 3 to 5v/v% 50wt% sucrose, 18 to 22 v/v% 50wt% glucose, 18 to 20v/v% ethyl acetate.
3. The use of claim 2, wherein the insect food attractant further comprises from 0.1 to 0.2v/v% of 2, 4-dihydroxybenzophenone, from 0.2 to 0.4v/v% of 2-hydroxy-4-n-octoxybenzophenone, and from 0.2 to 0.4v/v% of 6-tert-butyl-p-cresol.
4. The use of claim 1, wherein the insect food attractant is prepared by a process comprising the steps of:
the thujene is prepared into a solution with the concentration of 50v/v% -57 v/v% to obtain the insect feeding attractant.
5. The use of claim 2, wherein the insect food attractant is prepared by a process comprising the steps of: the insect food attractant is prepared by mixing thujaplicin, alcohol, 50wt% of sucrose, 50wt% of glucose and ethyl acetate according to the volume ratio of 50-57: 3-5: 18-22: 18-20.
6. The use of claim 3, wherein the insect food attractant is prepared by a process comprising the steps of: the insect phagostimulant is obtained by mixing thujaplicin, alcohol, 50wt% sucrose, 50wt% glucose, ethyl acetate, 2, 4-dihydroxybenzophenone, 2-hydroxy-4-n-octyloxybenzophenone and 6-tert-butyl-p-cresol at a volume ratio of 50-57: 3-5: 18-22: 18-20: 0.1-0.2: 0.2-0.4.
7. The use according to any one of claims 1 to 3, wherein the method of trapping insects by the insect food attractant is: the insect attractant is placed at an attracting core of the insect trap, 20-50 ml of 10wt% cane sugar water is filled at the bottom of the insect trap, the insect attractant is added once every 14 days, and meanwhile, the cane sugar water in a container is replaced.
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| CN112042643B true CN112042643B (en) | 2021-09-07 |
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| FR2678482A1 (en) * | 1991-07-01 | 1993-01-08 | Agronomique Inst Nat Rech | ATTRACTIVE COMPOSITIONS OF FEMALE LEPIDOPTERS TORTRICIDES. |
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| CN111096338A (en) * | 2019-12-12 | 2020-05-05 | 湖南省林业科学院 | Attractant for tea seed elephant |
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| CN101268786A (en) * | 2008-04-18 | 2008-09-24 | 浙江大学 | Winged insect attractant |
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| CN109730083A (en) * | 2019-02-02 | 2019-05-10 | 湖南省植物保护研究所 | Bemisia tabaci approach behavior adjusting control agent, the application of insecticide and insecticide, the application of desinsection plate and desinsection plate |
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