CN106688729B - Method for preventing and treating thrips pests by using pheromone and predatory natural enemies in combined mode - Google Patents

Method for preventing and treating thrips pests by using pheromone and predatory natural enemies in combined mode Download PDF

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CN106688729B
CN106688729B CN201611017458.3A CN201611017458A CN106688729B CN 106688729 B CN106688729 B CN 106688729B CN 201611017458 A CN201611017458 A CN 201611017458A CN 106688729 B CN106688729 B CN 106688729B
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thrips
pheromone
pests
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CN106688729A (en
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魏辉
田厚军
陈艺欣
陈勇
林硕
张艳璇
赵建伟
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Institute of Plant Protection of FAAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/02Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02Saturated carboxylic acids or thio analogues thereof; Derivatives thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
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  • Agronomy & Crop Science (AREA)
  • Insects & Arthropods (AREA)
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Abstract

The invention discloses a method for jointly preventing and treating thrips pests by utilizing pheromone and predatory natural enemy, which takes thrips aggregated pheromone as a main component, takes plant green leaf smell as a synergistic component, prepares a slow-release carrier by different proportions, and improves the probability of discovering and eating thrips by the predatory natural enemy in the process of attracting the thrips aggregated by (R) -lavandulyl, neryl (S) -2-methylbutanate, and the stability and the durability of the attracting rate pheromone aggregated by the thrips by adding cis-3-hexeneacetic acid ester, compared with the singly applied predatory natural enemy or pheromone, the invention improves the prevention and treatment effect by 28 to 45 percent, can reduce the using amount of chemical pesticides, reduce the damage rate of vegetables or flowers, protect the biological diversity and natural enemy resources, and develop a new means of the pheromone in the field of preventing and treating pests, opens up an important way for the comprehensive prevention and control technology system of the thrips pests.

Description

Method for preventing and treating thrips pests by using pheromone and predatory natural enemies in combined mode
Technical Field
The invention belongs to the technical field of agricultural pest control, and particularly relates to a method for controlling thrips pests by using pheromone and predatory natural enemies in a combined manner.
Background
Thrips is a general term for insects of the order Thysanoptera (Thysanoptera), which are tiny and often hidden in flowers and young leaves. Currently, 7700 and 570 species of thrips are recorded worldwide, and china accounts for more than 570 species [ major et al, 2011; mount et al, 2014 ]. Up to now, the species of thrips recorded all over the world are more than 2000, belonging to about 276 in the 4 subfamily, and 315 in the 79 genus in china [ huqingling, 2013 ]. Thrips have strong reproductive capacity, serious generation overlap and high outbreak frequency, the diffusion and harm of thrips pests are accelerated by global trade and climate warming, the thrips pests become one of the most main agricultural pest groups in the world gradually, and can spread various viruses including the spotted love virus and also are important external invasive pests for outbreak of disasters. Thrips feeding complexity including phytophagy, predation and bacteriophagy, wherein phytophagy thrips include harmful thrips that harm crops and beneficial thrips that control weeds; part of the thrips taeda is an important natural enemy of some small insects.
The plant volatile substances have effects of repelling and attracting insects, and killing some insects [ Dyeqing et al, 2010)]And has a synergistic effect with insect pheromones [ Selengchun et al, 2013]. Linalool oxide in Verbena plant has effect in attracting Frankliniella occidentalis, reducing toxic probability of thrips, and reducing harm [ Matsuuraet al.,2006]. Honeysuckle flower (1)Lonicera japonicaThunb) volatile substance cis-jasmoneThrips obscuratus(Crawford) has a remarkable attracting effect, but has a poor attracting effect on thrips tabaci [ El-Sayedet al., 2009]. Volatile substance methyl anthranilate for thrips palmi karny and thrips chromocor: (A)Thrips coloratusSchmutz), Frankliniella occidentalis (Thrips flavusSchrank) and Cirsium setosum both have good attractant effect [ Imai et al, 2001]. Eugenol, a plant secondary metabolite, slows down thrips activity and shortens spawning time, thereby reducing spawning amount and harm to crops [ Riefler et al, 2009]. The insect source information compound is used as a type of pheromone and plays an important role in regulating and controlling the behaviors of insects such as mating, gathering and the like. The insect population transmits information through the information compound to maintain the stability of the population. Insect pheromone as a non-chemical control measure can be used for monitoring the pest population dynamics and can be combined with other control measures to improve the pest control effect, and the insect pheromone control technology is also widely applied to the comprehensive control of pests. In recent years, the natural enemy also obtains one in the aspect of controlling thripsIn some progresses, under the current situation of outbreak and frequent occurrence of pests, one control measure is often difficult to solve all the problems, and particularly under the background that food safety and environmental protection are highly valued, the development of novel biological control is urgently needed.
The invention combines biological control and luring technology, and performs ecological control on the thrips in a mode of synergistic action of the thrips gathering pheromone, the natural enemies and the green leaf odor of plants, thereby playing a good control effect. Moreover, no report on the combined control of thrips by gathering pheromones and predatory natural enemies of thrips is found at present.
Disclosure of Invention
The invention aims to provide a method for jointly preventing and treating thrips pests by utilizing pheromone and predatory natural enemy, which is a method for jointly preventing and treating thrips pests by utilizing thrips gathering pheromone, plant green leaf odor and predatory natural enemy, and is realized by adopting the following technical scheme:
a method for preventing and controlling thrips pests by using pheromone and predatory natural enemies in a combined way comprises the following implementation steps: releasing the predatory natural enemies of the thrips into the thrips population, and simultaneously suspending the slow release carrier carrying the thrips pest pheromone compound on the thrips population by 0.01-0.5 m; so that the predatory natural enemies can prey on the thrips pests in the gathering process.
The predatory natural enemy is any one of orius pollicis guenee, Amara thistle, lacewing fly, trichogramma, and predatory mite.
The pheromone compound comprises the following components in percentage by mass: (R) -lavandulyl: neryl (S) -2-methylbutanate: cis-3-hexeneacetate = 1-3: 2-50: 0.1 to 0.5.
The preparation method of the slow release carrier carrying the thrips insect pheromone compound comprises the following steps: weighing (R) -lavandulyl, neryl (S) -2-methylbutanoate and cis-3-hexeneacetic ester according to the proportion, dissolving in normal hexane to prepare a slow release carrier solution with a certain concentration, adding 0.5mg of antioxidant 2, 6-di-tert-butyl-p-cresol and ultraviolet light adsorbent 2-hydroxy-4-methoxybenzophenone respectively, sealing in a polyvinyl chloride bag, and storing the slow release carrier in a refrigerator at 0-4 ℃.
It is another object of the present invention to provide the use of said method for improving the control of thrips pests by predatory natural enemies in vegetables or flowers. The invention combines the thrips agglutinin and the green leaf odor in an optimal proportion to form a slow release carrier which improves the effect of predatory natural enemies in preventing and controlling thrips pests; the slow release carrier and the predatory natural enemies are released simultaneously in the thrips high-incidence season in the flower or vegetable fields so as to achieve the purposes of controlling thrips, protecting the natural enemies and maintaining the biodiversity.
The invention discloses a method for jointly preventing and treating thrips pests by utilizing pheromone and predatory natural enemy, which takes thrips aggregated pheromone as a main component and plant green leaf smell as a synergistic component to prepare a slow-release carrier through different proportions, the probability of finding and eating the thrips by the predatory natural enemy is improved in the process of attracting the thrips to aggregate, the added cis-3-hexeneacetic acid ester can properly enhance the attraction rate of the thrips aggregated pheromone, and the stability and the lasting period of the released pheromone have better effect, thereby achieving the purpose of trapping and killing the pests. The prevention and control experiment proves that under the condition of using the invention, the prevention and control effect can be improved by 28-45% compared with the prevention and control effect of the predatory natural enemy or the pheromone which is independently applied, the technology of the invention has good effects on reducing the damage rate of vegetables or flowers, improving the quality of export flowers in China and protecting biodiversity and natural enemy resources, develops a new means of the pheromone in the field of preventing and controlling pests, and opens up an important way for a comprehensive prevention and control technical system of the thrips pests.
Detailed Description
The present invention is further illustrated by the following examples, but the scope of the present invention is not limited to the following examples.
Example 1: laboratory test
1) Taking 3 pots of indoor planted tomato plants, respectively marking as No. 1, No. 2 and No. 3, covering all tomato plants with an insect-proof net, placing the tomato plants in a laboratory, taking 50 western flower thrips bred indoors, respectively connecting the western flower thrips bred indoors to No. 1, No. 2 and No. 3 pot tomato plant leaves, respectively connecting one southern orius on the 1, No. 2 and No. 3 tomato plant leaves, and investigating results after eating 24 hours. The experiment was repeated three times, each time the released orius pollicis had to be consistent in age and individual to individual. The test result was designated as CK 1.
The investigation on the numbers of the frankliniella occidentalis of the tomato plants No. 1, No. 2 and No. 3 shows that the number of the frankliniella occidentalis on the tomato leaves of the tomato plant No. 1 is 38 +/-2.8, the number of the tomato plant leaves of No. 2 is 39 +/-6.6, and the number of the tomato plant leaves of No. 3 is 39 +/-2.9.
Example 2: laboratory test
1) Taking 3 pots of indoor planted tomato plants, respectively marking as No. 1, No. 2 and No. 3, covering all tomato plants with an insect-proof net, placing the tomato plants in a laboratory, taking 50 western flower thrips raised indoors, respectively connecting to No. 1, No. 2 and No. 3 pot tomato plant leaves, respectively connecting one green lacewing to the No. 1, No. 2 and No. 3 tomato plant leaves, and investigating the result after eating 24 hours. The test is repeated three times, and the lacewings released each time must be of consistent age and not very different individuals. The test result was designated as CK 2.
The investigation on the numbers of the frankliniella occidentalis of the tomato plants No. 1, No. 2 and No. 3 shows that the number of the frankliniella occidentalis on the tomato leaves of the tomato plant No. 1 is 31 +/-3.3, the number of the tomato plant leaves of No. 2 is 32 +/-6.1, and the number of the tomato plant leaves of No. 3 is 33 +/-2.0.
Example 3: laboratory test
1) Dissolving (R) -lavandulyl, neryl (S) -2-methylbutanoate and cis-3-hexeneacetic ester in normal hexane according to the mass ratio of 10:20:1 to prepare a slow-release carrier solution with a certain concentration, adding 0.5mg of antioxidant 2, 6-di-tert-butyl-p-cresol and ultraviolet light adsorbent 2-hydroxy-4-methoxybenzophenone respectively, sealing the carrier solution in a polyvinyl chloride bag, and placing the polyvinyl chloride bag in a refrigerator at 0-4 ℃ for later use.
2) Taking 3 pots of indoor planted tomato plants, respectively marking as No. 1, No. 2 and No. 3, covering all tomato plants with an insect-proof net, placing the tomato plants in a laboratory, taking 50 western flower thrips bred in the laboratory, respectively connecting the western flower thrips bred in the laboratory with No. 1, No. 2 and No. 3 pot tomato plant leaves, respectively connecting one southern orius on the 1, No. 2 and No. 3 tomato plant leaves, simultaneously hanging a compound slow release carrier containing thrips gathering pheromone and green leaf smell at a position 3cm above the tomato plants, and waiting for eating 24h and investigating results. The experiment was repeated three times, each time the released orius pollicis had to be consistent in age and individual to individual.
The investigation on the number of thrips of tomato plants No. 1, No. 2 and No. 3 shows that the number of thrips on the tomato leaves of the tomato plant No. 1 is 24 +/-4.3, the number of the tomato plant No. 2 is 22 +/-3.1, and the number of the tomato plant No. 3 is 21 +/-4.7.
Example 4: laboratory test
1) Dissolving (R) -lavandulyl, neryl (S) -2-methylbutanoate and cis-3-hexeneacetic ester in normal hexane according to the mass ratio of 2:18:0.3 to prepare a slow-release carrier solution with a certain concentration, adding 0.5mg of antioxidant 2, 6-di-tert-butyl-p-cresol and ultraviolet light adsorbent 2-hydroxy-4-methoxybenzophenone respectively, sealing in a polyvinyl chloride bag, and placing in a refrigerator at 0-4 ℃ for later use.
2) Taking 3 pots of indoor planted tomato plants, respectively marking as No. 1, No. 2 and No. 3, covering all tomato plants with an insect-proof net, placing the tomato plants in a laboratory, taking 50 western flower thrips raised indoors, respectively connecting the western flower thrips raised indoors to No. 1, No. 2 and No. 3 pot tomato plant leaves, respectively connecting one green lacewing to the No. 1, No. 2 and No. 3 tomato plant leaves, simultaneously hanging a compound slow release carrier containing thrips gathering pheromone and green leaf smell above the tomato plants by 3cm, and investigating results after eating 24 hours. The test is repeated three times, and the lacewings released each time must be of consistent age and not very different individuals.
The investigation on the number of thrips of tomato plants No. 1, No. 2 and No. 3 shows that the number of thrips on the tomato leaves of the tomato plant No. 1 is 15 +/-2.1, the number of the tomato plant No. 2 is 18 +/-3.0, and the number of the tomato plant No. 3 is 16 +/-3.2.
Example 5: laboratory test
1) Dissolving (R) -lavandulyl, neryl (S) -2-methylbutanoate and cis-3-hexeneacetic ester in normal hexane according to the mass ratio of 2:30:0.4 to prepare a slow-release carrier solution with a certain concentration, adding 0.5mg of antioxidant 2, 6-di-tert-butyl-p-cresol and ultraviolet light adsorbent 2-hydroxy-4-methoxybenzophenone respectively, sealing in a polyvinyl chloride bag, and placing in a refrigerator at 0-4 ℃ for later use.
2) Taking 3 pots of indoor planted tomato plants, respectively marking as No. 1, No. 2 and No. 3, covering all tomato plants with an insect-proof net, placing the tomato plants in a laboratory, taking 50 western flower thrips raised indoors, respectively connecting the western flower thrips raised indoors to No. 1, No. 2 and No. 3 pot tomato plant leaves, respectively connecting one green lacewing to the No. 1, No. 2 and No. 3 tomato plant leaves, simultaneously hanging a compound slow release carrier containing thrips gathering pheromone and green leaf smell above the tomato plants by 3cm, and investigating results after eating 24 hours. The test is repeated three times, and the lacewings released each time must be of consistent age and not very different individuals.
Investigation of the number of thrips of tomato plants No. 1, No. 2 and No. 3 shows that the number of thrips on the tomato leaves of the tomato plant No. 1 is 13 +/-2.5, the number of the tomato plant leaves of No. 2 is 19 +/-2.1, and the number of the tomato plant leaves of No. 3 is 11 +/-2.8.
Example 6: field test
1) In the season of high incidence of Frankliniella occidentalis, a 60m field is selected from the plant protection institute of rural institute of Fujian province2The tomato test field has 9 plots, 9 plots in each plot, 81 tomatoes in each plot, 3 plots as a cell, 3 repeats of 3 plots, and is marked as cells A, B and C which are all used as CK 3. Before the test is started, the number of frankliniella occidentalis in each test field of each cell is firstly investigated, and the number of frankliniella occidentalis in a cell A is 250 heads, the number of a cell B is 270 heads, and the number of a cell C is 300 heads after investigation.
2) The orius pollidipennis is released in each cell, 1 orius pollidipennis is placed in each 3 tomatoes, and the orius pollidipennis should be consistent in age and not very different in individual. The test results were investigated after 24 h.
The number of insects was investigated after 24h, and the results showed that the total number of frankliniella occidentalis in cell a was 185, the total number of frankliniella occidentalis in cell B was 201, and the total number of frankliniella occidentalis in cell C was 223.
Example 7: field test
1) In the season of high incidence of Frankliniella occidentalis, a 60m field is selected from the plant protection institute of rural institute of Fujian province2The tomato test field has 9 plots, 9 plots in each plot, 81 tomatoes in each plot, 3 plots as a cell, 3 repeats of 3 plots, and is marked as cells A, B and C which are all used as CK 4. Each cell trial is investigated before the trial beginsThe number of frankliniella occidentalis in the field is tested, and the number of frankliniella occidentalis in the investigation community A is 250, the number of the community B is 270, and the number of the community C is 300.
2) Every cell releases green lacewings, every 3 tomatoes release 1 green lacewing, and the green lacewings should be of the same age and have small individual difference. The test results were investigated after 24 h.
The number of insects was investigated after 24h, and the results showed that the total number of frankliniella occidentalis in cell a was 175, the total number of frankliniella occidentalis in cell B was 186, and the total number of frankliniella occidentalis in cell C was 199.
Example 8: field test
1) Dissolving (R) -lavandulyl, neryl (S) -2-methylbutanoate and cis-3-hexeneacetic ester in normal hexane according to the mass ratio of 10:20:1 to prepare a slow-release carrier solution with a certain concentration, adding 0.5mg of antioxidant 2, 6-di-tert-butyl-p-cresol and ultraviolet light adsorbent 2-hydroxy-4-methoxybenzophenone respectively, sealing the carrier solution in a polyvinyl chloride bag, and placing the polyvinyl chloride bag in a refrigerator at 0-4 ℃ for later use.
2) In the season of high incidence of Frankliniella occidentalis, a block of 60m with the same area and arrangement as CK3 and the same number of tomatoes is selected at a distance of CK30m2The tomato test field is treated by 9 ridges, 9 tomatoes in each ridge, 81 tomatoes in each ridge, 3 ridges as one cell, 3 cells are divided, namely 3 times of repetition are recorded as a cell A1,B1,C1. Before the test is started, the number of frankliniella occidentalis in each plot is investigated, and the plot A is investigated1The number of frankliniella occidentalis is 260, and the cell B1Is 310 head, cell C1Is 320 heads.
3) Two slow release carriers are hung in the first furrow of each cell, the distance is 5m, the distance is 3cm from the leaf of each tomato, the orius pollidipennis is released simultaneously, 1 orius pollidipennis is placed in each 3 tomatoes, and the orius pollidipennis should be consistent in age and have small individual difference. The test results were investigated after 24 h.
The number of insects is investigated after 24h, and the result shows that the cell A1The total frankliniella occidentalis number is 158, cell B1Total Frankliniella occidentalis number 179, cell C1The total frankliniella occidentalis number is 180.
Example 9: field test
1) Dissolving (R) -lavandulyl, neryl (S) -2-methylbutanoate and cis-3-hexeneacetic ester in normal hexane according to the mass ratio of 2:18:0.3 to prepare a slow-release carrier solution with a certain concentration, adding 0.5mg of antioxidant 2, 6-di-tert-butyl-p-cresol and ultraviolet light adsorbent 2-hydroxy-4-methoxybenzophenone respectively, sealing in a polyvinyl chloride bag, and placing in a refrigerator at 0-4 ℃ for later use.
2) In the season of high incidence of Frankliniella occidentalis, a block of 100m with the same area and arrangement as CK4 and the same number of tomatoes is selected at the position of CK45m2The tomato test field is treated by 9 ridges, 9 tomatoes in each ridge, 81 tomatoes in each ridge, 3 ridges as one cell, 3 cells are divided, namely 3 times of repetition are recorded as a cell A2,B2,C2. Before the test is started, the number of frankliniella occidentalis in each plot is investigated, and the plot A is investigated2The number of frankliniella occidentalis is 270, and the cell B2325 head, cell C2Is 300 heads.
3) Two slow release carriers are hung in the first furrow of each cell, the distance is 5m, the distance is 3cm from the leaf of each tomato, the southern bug is released simultaneously, 1 lacewing is put in each 3 tomatoes, and the lacewings are consistent in age and have small individual difference. The test results were investigated after 24 h.
The number of insects is investigated after 24h, and the result shows that the cell A2The total frankliniella occidentalis number is 135, and the cell B2The total frankliniella occidentalis number is 159, cell C2The total frankliniella occidentalis number is 136.
Example 10: field test
1) Dissolving (R) -lavandulyl, neryl (S) -2-methylbutanoate and cis-3-hexeneacetic ester in normal hexane according to the mass ratio of 2:30:0.4 to prepare a slow-release carrier solution with a certain concentration, adding 0.5mg of antioxidant 2, 6-di-tert-butyl-p-cresol and ultraviolet light adsorbent 2-hydroxy-4-methoxybenzophenone respectively, sealing in a polyvinyl chloride bag, and placing in a refrigerator at 0-4 ℃ for later use.
2) In the season of high incidence of Frankliniella occidentalis, a block of 100m with the same area and arrangement as CK4 and the same number of tomatoes is selected at the position of CK60m2The tomato test field is treated for 9 plots, each plot has 9 plants and the total number of the plots is 81 tomato, 3 ridges as one cell, 3 cells as 3 repeats, and recording as cell A3,B3,C3. Before the test is started, the number of thrips in each plot is investigated, and the plot A is investigated3The number of Frankliniella occidentalis is 250, and the cell B3Is 245 head, cell C3290 heads.
3) Two slow release carriers are hung in the first furrow of each cell, the distance is 5m, the distance is 3cm from the leaf of each tomato, the southern bug is released simultaneously, 1 lacewing is put in each 3 tomatoes, and the lacewings are consistent in age and have small individual difference. The test results were investigated after 24 h.
The number of insects is investigated after 24h, and the result shows that the cell A3The total frankliniella occidentalis number is 114, and cell B3The total frankliniella occidentalis number is 158, cell C3The total frankliniella occidentalis number is 140.
The results show that in the indoor test, after the southern orius bugus takes the food for 24 hours, the total amount of CK Frankliniella occidentalis is 116 heads; after the compound sustained-release carrier containing the thrips aggregation pheromone and the plant green leaf odor is hung, the total amount of the frankliniella occidentalis is lower than 65 heads, and is obviously reduced compared with CK, and the closer to the sustained-release carrier, the larger the food intake of the orius minutus is. In a field test, after only the orius miniatus is released, the total residual quantity of CK Frankliniella occidentalis is 609 heads, and is not remarkably reduced compared with 820 heads before release; and after the compound sustained-release carrier containing the thrips aggregatory pheromone and the plant green leaf odor is hung, the total amount of the frankliniella occidentalis is obviously reduced compared with CK. The result of the green lacewing eating for 24h is similar to the predation effect of the southern orius.

Claims (3)

1. A method for preventing and treating thrips pests by using pheromone and predatory natural enemies in a combined manner is characterized in that the predatory natural enemies of thrips are released into thrips populations, and meanwhile, a slow release carrier carrying a thrips pest pheromone compound is suspended at 0.01-0.5 m of the thrips populations; predatory natural enemies are predated in the gathering process of the thrips pests; the pheromone compound comprises the following components in percentage by mass: (R) -lavandulyl: neryl(s) -2-methylbutanate: cis-3-hexeneacetate =10:20:1 or 2:18:0.3 or 2:30: 0.4;
the preparation method of the slow release carrier carrying the thrips insect pheromone compound comprises the following steps: weighing (R) -lavandulyl, neryl (S) -2-methylbutanoate and cis-3-hexeneacetic ester according to the proportion, dissolving in normal hexane to prepare a slow release carrier solution with a certain concentration, adding 0.5mg of antioxidant 2, 6-di-tert-butyl-p-cresol and ultraviolet light adsorbent 2-hydroxy-4-methoxybenzophenone respectively, sealing in a polyvinyl chloride bag, and storing the slow release carrier in a refrigerator at 0-4 ℃.
2. The method for controlling thrips pests using a pheromone in combination with a predatory natural enemy according to claim 1, wherein the predatory natural enemy is any one of orius miri, thrips taeniaca, phlebophora, trichogramma, predatory mites.
3. The method for controlling thrips pests in vegetable and flower planting by using pheromone and predatory natural enemy in combination as claimed in claim 1.
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CN107333596B (en) * 2017-06-20 2020-08-11 北京市植物保护站 Method for preventing and treating watermelon thrips by utilizing orius sauteri
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