CN117814255A - Bemisia tabaci plant source attractant and application thereof - Google Patents

Bemisia tabaci plant source attractant and application thereof Download PDF

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Publication number
CN117814255A
CN117814255A CN202311804208.4A CN202311804208A CN117814255A CN 117814255 A CN117814255 A CN 117814255A CN 202311804208 A CN202311804208 A CN 202311804208A CN 117814255 A CN117814255 A CN 117814255A
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attractant
adult
attracting
composition
bemisia
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陈珍珍
贾志飞
许永玉
李丹丹
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Shandong Agricultural University
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Shandong Agricultural University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention belongs to the technical field of biological prevention and control of cash crops, and provides a bemisia tabaci plant source attractant and application thereof. An attractant composition for adult black thorn whitefly is prepared from one of farnesene and leaf alcohol and linalool. The attracting composition is placed at the tea tip in the emergence period of overwintering generation and final generation of the adult black thorn whiteflies, so that the adult black thorn whiteflies can be attracted efficiently, and the attracting composition can be prepared into preparation forms such as attracting cores and the like and can be combined with yellow sticky trap. The attractant composition provided by the invention can radically depress the number of the whole-year worms and can reduce or avoid the use of chemical agents. The invention is beneficial to protecting the zoo population ecology, has the characteristics of green, safe and efficient, is convenient and quick to use, has lower cost and is suitable for actual production practice.

Description

Bemisia tabaci plant source attractant and application thereof
Technical Field
The invention belongs to the technical field of biological prevention and control of cash crops, and particularly relates to a plant source attractant for aleyrodis Niger and application thereof.
Background
This background information is disclosed to enhance an understanding of the general background of the invention and is not necessarily to be considered an admission or any form of suggestion that this information constitutes prior art already known to a person of ordinary skill in the art.
The aleyrodis nigrum belongs to the order of hemiptera, the family of aleyrodidae and the genus aleyrodid, and is an important pest for the tea tree worldwide. In the 60-90 th century, aleyrodid was intermittently outbreaked in the middle and downstream of the Yangtze river, south China and south China, etc. tea areas in China, and then serious in most tea gardens in the south China, the southern Anhui, the south China, the north Guangdong and the north China, and becomes a common important pest in tea gardens. Adults like to gather on tender leaves at the upper part of tea trees for spawning, and after egg hatching, except for 1-year-old nymphs, other nymphs are fixed and sucked in a piercing mode to be harmful, so that the yield and quality of tea are directly affected. The excrement of the insects can also induce tea coal diseases, reduce photosynthesis of tea leaves and influence the growth of tea trees. Because the generation overlapping of the aleyrodis nigrum is serious, and the wall of the nymph is thicker, the pest control is more difficult.
Chemical control is the most common method for controlling the black thorn whiteflies at home and abroad, and pyrethroid and neonicotinoid medicaments are used as main medicaments, so that natural enemy insects can be killed by using the insecticide, the population ecology of tea gardens is destroyed, and meanwhile, the safe production of tea leaves is influenced, and therefore, the search of a new efficient green black thorn whitefly control technology is urgent. The plant source attractant is a chemical component with insect attracting activity screened from plant volatile matters, and has the characteristics of safety, high efficiency, green and the like compared with chemical pesticides. The plant source attractant is developed for insects with prevention and control requirements, the trapping efficiency of target insects can be improved, the prevention and control of pests are more accurate, the broad-spectrum killing effect of chemical agents on natural enemy insects and non-target insects is avoided, the biological diversity of ecological environment is protected, and meanwhile the problems of food safety, insect drug resistance and the like caused by excessive use of the chemical agents are effectively solved.
Insects find host plants that are favorable to their growth and reproduction by smell, and the plants influence the search and location of insects by releasing volatiles. The investigation shows that the occurrence amount of the black thorn whiteflies on different resistant tea tree varieties is greatly different, and the difference is derived from the selection preference of the black thorn whitefly adults on different varieties of volatile matters. If the compound with strong attracting effect on the adult black thorn whiteflies is screened out from the volatile matters of different insect-resistant tea tree varieties, the insect-resistant tea tree can be prepared into the adult black thorn whiteflies attractant, and can be timely prevented and controlled at the early stage of insect pest occurrence, so that the annual insect population is depressed. Yellow insect attracting boards are common physical pest control technology and are often used as a large-scale control means for the aleyrodid Niger. If the adult black thorn whitefly attractant is used in combination with the yellow board, the accuracy of trapping the black thorn whitefly by the yellow board can be greatly improved, and a novel physicochemical induction control technology is provided for accurately monitoring, preventing and controlling the black thorn whitefly due to the synergistic effect of the adult black thorn whitefly attractant and the yellow board.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a plant source attractant for the adult black thorn whiteflies, which comprises the following components: the attractant can efficiently attract adults, is beneficial to protecting the zoology of tea garden population, has the characteristics of green, safe and efficient, is convenient and quick to use, has lower cost, and is suitable for actual production practice.
The invention also aims to provide the application of the plant source attractant in trapping the black thorn whiteflies, and the plant source attractant can be placed at tea tips in the emergence period of overwintering generation and final generation of the black thorn whiteflies, so that the number of whole years of insects can be reduced from root sources, and the use of chemical agents can be reduced or avoided.
In order to achieve the above purpose, the present invention adopts the following technical scheme.
An attracting composition for adult black thorn whiteflies comprises the following active ingredients:
(a) Linalool; and
(b) One of farnesene or leaf alcohol;
(a) The mass ratio of the component (a) to the component (b) is 3:1.
The above-described attractant composition may be used to lure adult black lice by adsorbing or loading onto an inert carrier to prepare an attractant. The attractant composition or the attractant prepared from the attractant composition can be used for monitoring or preventing and controlling the aleyrodid. The inert carrier can be paper, rubber, plastic, active carbon, porous mineral adsorbent such as montmorillonite, white carbon black, attapulgite, diatomite, etc. The attractant may also include an insecticide.
The invention also provides a method for attracting the aleyrodid nigrum through the attraction composition or the attractant, which comprises the following steps: the attractant composition or attractant is placed at the tea tip during the emergence period of overwintering generation and final generation of the adult black thorn whiteflies. In order to enhance the attracting effect, the attracting agent can be combined with other attracting modes, such as combining the attracting composition or the attracting agent with a yellow sticky trap. In some embodiments, the above-mentioned attractant composition or attractant is attached to yellow sticky trap to form a physicochemical combined trapping device, the device is placed at a distance of 5-10-cm a from tea, the dosage of effective component is 1-2mg per square meter, and the device can be periodically replaced according to the occurrence of adult black thorn whiteflies, and removed or replaced after about 15-30 days.
The invention has the following advantages:
the invention screens and obtains the composition with good attracting effect on the adult black thorn whiteflies, and the composition and the yellow insect attracting plate form a physicochemical combined trapping device for trapping the first-generation and the last-generation adults of the black thorn whiteflies, so that the number of the whole-year worms can be reduced from the root, and the use of chemical agents can be reduced or avoided. The plant source attractant is natural volatile chemical components of plant sources, does not destroy the zoology of tea garden population, and is a novel green, safe and efficient attractant. The plant source attractant has a simple formula, and the lure core and physicochemical combined trapping device is convenient and quick to use, has low cost and is suitable for actual production practice.
Drawings
FIG. 1 is a graph showing the response of adult Bemisia tabaci to the antennal potential (EAG) of phyllitol, linalool, and farnesene, wherein the same letter in the same table indicates that the difference is insignificant (p > 0.05), and the different letter indicates that the difference is significant (p <0.05, tukey method);
fig. 2 shows a physicochemical combined trapping device composed of a white fly adult attractant lure core and a yellow lure plate.
Detailed Description
The present invention will be further illustrated with reference to the following examples, but the present invention is not limited to the following examples.
Example 1 screening of the proportions and concentrations of attractant Compounds
1. Screening of attractant compound concentrations
Leaf alcohol, linalool and farnesene are selected as components of the bemisia tabaci attractant, and the optimal concentration of the bemisia tabaci adult attractant is screened by using an antenna potential (EAG) technology, and the specific method is as follows: the black thorn whitefly tentacles were connected to both ends of the Ag-AgCl electrode, n-hexane was used as solvent, and 1, 10, 100, 250 and 500 μg/μl of leaf alcohol, linalool and farnesene were used as the source of the stimulating taste, and the EAG response values of the tentacles after each odor stimulation were recorded.
As can be seen from the results of fig. 1: the plant-derived attractants leaf alcohol, linalool and farnesene all caused the adult black thorn whitefly to produce an antennal potential response, and the antennal electrophysiological response was most intense at a concentration of 100 μg/μl, so the attractant concentration was determined to be 100 μg/μl in field trials.
2. Screening of the proportion of attractant Compounds
Leaf alcohol, linalool and farnesene are combined in pairs to be used as attractants, namely farnesene+linalool, leaf alcohol+linalool, farnesene+leaf alcohol are respectively adopted, and 3 proportions of the attractants are 1:3,1:1 and 3:1. The optimal proportion of the adult Bemisia tabaci attractant is screened by using an antenna potential (EAG) technology, and the specific method is as follows: the black thorn whitefly tentacles were connected to both ends of the Ag-AgCl electrode, the attractants were diluted to 100 μg/μl with n-hexane as a solvent, 3 ratios of the above 3 attractants were used as the source of the stimulating odor, and the EAG response values of the tentacles after each odor stimulation were recorded.
TABLE 1 Effect of attractant ratios on the electrophysiological responses of Bemisia Niger adults
Note that: data in the same row are not significantly different (p > 0.05) by the same letter, and are significantly different (p <0.05, tukey method).
As can be seen from the results in Table 1,3 combinations (farnesene+linalool, leaf alcohol+linalool, farnesene+leaf alcohol) at a concentration of 100. Mu.g/. Mu.L all caused the antenna electrophysiological response of the adult Bemisia tabaci at 3 ratios (1:3, 1:1, 3:1), with farnesene+linalool ratios of 1:3, leaf alcohol+linalool ratios of 1:3 and farnesene+leaf alcohol ratios of 1:1 being the strongest antenna electrophysiological response.
Example 2 field trials of plant attractants for adult Bemisia Niger
The test was carried out in an ecological tea garden of to Wen of Taian city, shandong province, 10 months and 9 days in northern arborvitae village of Mawan, which was not applied with chemical pesticide throughout the year, and was planted as Fuding white tea.
Table 2 plant attractant for aleyrodis nigrum
According to the components and mass ratio shown in Table 2, the attractant composition was diluted to 100. Mu.g/. Mu.L using n-hexane as a solvent, and 0.5. 0.5g attractant component was added to each empty rubber head attractant core to prepare a Bemisia tabaci plant source attractant. 3 tea sheds with the same tea tree growth vigor are selected as 3 repeated test cells, the area of each cell is about 5 mu, the intervals between different cells exceed 15 m, and the following devices are randomly placed in each cell for processing:
(1) Physicochemical combined trapping: consists of an attractant lure and a yellow sticky trap (figure 2);
(2) Individual attractants: consists of an attractant lure core and a white insect sticking plate;
(3) Control: yellow plates without attractant wick.
The device is placed at a distance of 5-10: 10 cm from the tea, each 25: 25 m 2 1 was placed. Every 7-10 days, investigating the insect attracting quantity on the mythic fungus plate and changing new mythic fungus plate, and investigating 4 times (investigating insect attracting quantity and changing mythic fungus plate time: 2022, 10, 16, 7d, 2022, 10, 23 days ]14d) And 2022, 11, 1 (23 d); end time of test: 2022, 11, 8 (30 d)).
Data from field insect attracting tests were analyzed by one-way analysis of variance (ANOVA) (p < 0.05). Multiple test of Tukey (p < 0.05) was used to compare differences between averages in pairs, fold increase and synergy ratio were calculated according to the following formula:
fold increase = (physicochemical combined trap number-number of attractants alone)/number of attractants alone;
synergistic ratio = (physicochemical combined trap number-control number)/control number x 100%.
TABLE 3 synergistic Effect test results of Bemisia Niger plant-derived attractants and yellow plates
Note that: data in the same row are not significantly different (p > 0.05) by the same letter, and are significantly different (p <0.05, tukey method).
From the results in Table 3, it can be seen that: the trapping effect of the plant source attractant for the adult black thorn whiteflies is greatly improved after the attractant core is combined with the yellow board, compared with the attractant which is singly used, the trapping amount of leaf alcohol and linalool (1:3) is increased by 70.8 times after the attractant core is combined with the yellow board, and the trapping amount of farnesene and linalool (1:3) is increased by 70.0 times.
TABLE 4 synergistic Effect of Bemisia Niger plant-derived attractants on yellow plates test results
From the results in Table 4, it can be seen that: the plant source attractant for the adult black thorn whiteflies can improve the trapping effect of the yellow board, the combination of the leaf alcohol and the linalool (1:3) can improve the trapping amount of the yellow board by 183.1 percent, and the trapping amount of the yellow board after carrying the attractant core of farnesene and linalool (1:3) is improved by 158.1 percent.
TABLE 5 trapping amount of physicochemical combined trapping device
Note that: the data in the same column were not significantly different by the same letter (p > 0.05) and were significantly different by the different letter (p <0.05, tukey method).
From the results in Table 5, it can be seen that: the physicochemical combined trapping device has obvious attracting effect on the 7 th to 23 th days, the average number of the adults for trapping the aleyrodis Niger is higher than that of a blank control, and the trapping effect on the 23 rd to 30 th days is reduced. The number of farnesene + linalool (1:3) and leaf alcohol + linalool (1:3) trapping devices trapped the adult whiteflies was significantly higher than the control device (p < 0.05) on days 7 and 14 after the lure setup, the average number of trapped adult whiteflies on day 7 was 8334.0 and 9144.0 heads respectively, and the average number of trapped adult whiteflies on day 14 was still up to 5556.0 and 6096.0 heads significantly higher than the control device (day 7: 3229.4 head; day 14: 2152.9 heads).
Example 3 preparation of yellow Sophora Niger luring plate
Preparing farnesene and linalool into mother liquor according to a mass ratio of 1:3 by using normal hexane, diluting by using ethanol, spraying the mother liquor onto a starch-based degradable plastic yellow board with the size of A4 paper to enable the effective dosage to reach 0.3 g/sheet, spraying glue after the solvent volatilizes, attaching release paper, filling into a packaging bag and sealing to obtain the black thorn whitefly attracting yellow board.
Example 4 preparation of a Bemisia Niger lure
Preparing solution of leaf alcohol and linalool according to a mass ratio of 1:3, adding the solution into a core-attracting rubber head to load effective dose of 0.5 g/L, and waiting for solvent volatilization to obtain the black thorn whitefly core-attracting.
Example 5 preparation of Bemisia Nippon Tinctorius attractant
The leaf alcohol and linalool are prepared into a solution by taking n-hexanol as a solvent according to the mass ratio of 1:3, then sprayed on EVA plastic particles through spraying, and the attractant is obtained after drying, and can be matched with a trapper for insect pest monitoring.
Example 6 preparation of Bemisia Nitidissma attractant
The farnesene and linalool are prepared into a solution by taking n-hexanol as a solvent according to the mass ratio of 1:3, then sprayed on EVA plastic particles through spraying, and the attractant is obtained after drying, and can be matched with a trapper for insect pest monitoring.
Example 7 preparation of Bemisia Nitidissma attractant
The farnesene and the linalool are mixed according to the mass ratio of 1:3, and then added into 10% bifenthrin emulsifiable concentrate according to the mass ratio of 0.5%, and the mixture is uniformly mixed to obtain the attractant, and the attractant can be matched with the trapper for preventing and controlling the black thorn whiteflies.

Claims (10)

1. The adult black thorn whitefly attracting composition is characterized by comprising the following active ingredients:
(a) Linalool; and
(b) One of farnesene or leaf alcohol;
(a) The mass ratio of the component (a) to the component (b) is 3:1.
2. An attractant prepared from the bemisia tabaci adult attractant composition of claim 1.
3. An attractant according to claim 2, further comprising an inert carrier.
4. An attractant as in claim 3, wherein the inert carrier is selected from at least one of paper, rubber, plastic, activated carbon, and porous mineral-based adsorbents.
5. An attractant according to claim 4, wherein the porous mineral-based adsorbent is selected from at least one of montmorillonite, white carbon, attapulgite, and diatomaceous earth.
6. An attractant as in claim 2, further comprising a pesticide.
7. Use of an adult bemisia tabaci attractant composition according to claim 1, an attractant according to any one of claims 2 to 6 for bemisia tabaci monitoring or control.
8. A bemisia nigra adult attractant composition according to claim 1, a method of attracting bemisia nigra by an attractant according to any one of claims 2 to 6, comprising the steps of: the attractant composition or attractant is placed at the tea tip during the emergence period of overwintering generation and final generation of the adult black thorn whiteflies.
9. A bemisia nigra adult attractant composition according to claim 1, a method of attracting bemisia nigra by an attractant according to any one of claims 2 to 6, comprising the steps of: the attractant composition or the attractant is attached to the yellow sticky trap and is placed at the tea tip in the emergence period of the overwintering generation and the final generation of the adult black thorn whiteflies.
10. The method of claim 9, wherein the yellow armyworm plate is placed at a distance of 5-10 cm from the tea leaf, and the dosage of the active ingredient is 1-2mg per square meter.
CN202311804208.4A 2023-12-26 2023-12-26 Bemisia tabaci plant source attractant and application thereof Pending CN117814255A (en)

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