CN114831116A - Application of acetic acid leaf alcohol ester in preventing and treating meloidogyne incognita - Google Patents

Application of acetic acid leaf alcohol ester in preventing and treating meloidogyne incognita Download PDF

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CN114831116A
CN114831116A CN202210370540.3A CN202210370540A CN114831116A CN 114831116 A CN114831116 A CN 114831116A CN 202210370540 A CN202210370540 A CN 202210370540A CN 114831116 A CN114831116 A CN 114831116A
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meloidogyne incognita
acetate
leaf alcohol
treatment
corn
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CN114831116B (en
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胡凌飞
欧阳筱
吴圳玮
徐建明
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Zhejiang University ZJU
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Biodiversity & Conservation Biology (AREA)
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  • Engineering & Computer Science (AREA)
  • Forests & Forestry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Toxicology (AREA)
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  • Pest Control & Pesticides (AREA)
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Abstract

The invention discloses an application of acetic acid leaf alcohol ester in preventing and treating meloidogyne incognita, which comprises the following steps: the corn plants are treated by using the slow release device to release the acetic acid leaf alcohol ester, and the treatment concentration is 70 +/-10 ng h per plant ‑1 The treatment time was 1.5. + -. 0.5 hours. The invention discovers for the first time that the exposure treatment of the leaf alcohol acetate can effectively inhibit the infection and the propagation of the meloidogyne incognita and obviously reduce the feeding preference of the meloidogyne incognita to host plants. The folyl acetate can inhibit the invasion and feeding of meloidogyne incognita by promoting the content of abscisic acid, jasmonic acid and jasmonic acid isoleucine conjugate, which are related hormones of plant root defense. In the application process, the folic acetate is safe, nontoxic, economic and environment-friendlyAfter treatment, no negative influence is brought to the growth of crops.

Description

Application of acetic acid leaf alcohol ester in preventing and treating meloidogyne incognita
Technical Field
The invention relates to the field of prevention and control of meloidogyne incognita, in particular to application of folic alcohol acetate in prevention and control of meloidogyne incognita.
Background
The plant root-knot nematode disease is one of the most serious soil-borne diseases in global agricultural production, and the root-knot nematode disease not only can cause the serious reduction of the yield and the quality of crops, but also can aggravate the occurrence of diseases such as bacteria, fungi and the like, and cause continuous cropping obstacles. According to incomplete statistics, the economic loss caused by nematode diseases worldwide every year is up to $ 1250 billion. How to effectively prevent and treat the root-knot nematode disease is an urgent problem to be solved in global agricultural production.
The root-knot nematode is exclusively parasitic in the plant body and has extremely strong adaptability and transmissibility. Currently, the level of root-knot nematode damage in four major pathogenic organisms responsible for plant infectious diseases has surpassed that of bacteria and viruses, second only to pathogenic fungi. Among them, Meloidogyne incognita (Meloidogyne incognita), Meloidogyne arachidis (Meloidogyne arenaria), Meloidogyne hapla (Meloidogyne hapla), Meloidogyne javanica (Meloidogyne javanica), and the like are mainly common four kinds of Meloidogyne. In China, the southern root-knot nematode has the most serious harm to crops. The life history of the southern root-knot nematode in plants is divided into three stages of eggs, larvae and adults, and the adults generally carry out parthenogenesis. Under the condition of proper temperature (20-30 ℃), the worm eggs are divided into about 20 cells to enter into a blastocyst stage after 2 days, enter into a prototheca stage after 4 or 5 days, and after 4-5 days of development, the worm eggs are formed into first-instar larvae through oral acupuncture, and after static settlement and first molting, the second-instar larvae are formed through development. The second instar larvae are larvae with infection capability and can freely move, infected parts are mainly fibrous roots and lateral roots of plants, and after the root-knot nematodes in the second instar stage establish feeding sites, the second instar larvae continue to develop into third instar larvae and fourth instar larvae until female adults. After the host plant is infected by the meloidogyne incognita, root cells proliferate to form multinucleate and swollen giant cells. Giant cells are the only source of nematode nutrients, and the proliferation of surrounding root cells leads to the formation of tumor masses, namely root knots, with uneven sizes on the root system. At the later stage of infection, more female insects are in the root knots, yellow or brown oocysts are outside the root knots, and at the moment, the root knots are gradually decayed, and the larvae begin to be released into the soil.
Corn is the cereal crop with the widest planting range and the largest yield all over the world and is one of the most important grain and feed crops in China. However, as the field reimplantation index of corn increases, root knot nematode disease in corn often occurs and tends to increase year by year. After the southern root-knot nematode infects the corn, the growth and development of the corn root system can be inhibited, and further the number of the corn roots is reduced, so that the corn plants grow badly. When the harm is serious, the root system of the corn is lignified, the overground plants are withered and yellow, and even the corn is dead, so that the corn cannot be harvested. Therefore, in the corn planting process, high attention needs to be paid to the control of meloidogyne incognita.
At present, the main control measures of the corn meloidogyne incognita mainly comprise two measures, one measure is an agricultural control measure, and the other measure is a medicament control measure. However, agricultural control is not effective in controlling root-knot nematode infestation. Although the traditional chemical pesticide can control nematode diseases, the traditional chemical pesticide can damage a soil ecosystem and cause environmental pollution, thereby influencing human and animal health. With the increasing public awareness of environmental protection and the increasing resistance of nematodes, the measures for preventing and treating the nematode by the pesticide are increasingly restricted. Therefore, in recent years, the search for plant-derived nematicides and the development of green sustainable control means have become the focus and emphasis of the control of meloidogyne incognita.
Folyl acetate is a volatile organic compound that is widely present in various plants and is released in large quantities when the plant is subjected to biotic or abiotic stress. Researches show that the acetic acid leaf alcohol ester can be used as an early warning signal substance to stimulate the immunity and resistance of plants and enhance the disease resistance and insect resistance of the plants. In addition, the folic acid esters have a "green" odor, can impart aroma and flavor to natural foods such as vegetables and fruits, and are widely used as antioxidants, coloring agents, aromatic agents and flavoring agents. Although the acetic acid phyllyl ester has wide application prospect in pest control and industrial biotechnology, the report of the acetic acid phyllyl ester for controlling meloidogyne incognita has not been found yet.
CN105578879A discloses a composition containing a harmful arthropod attractant containing a plant-derived component and an analog thereof, and provides a control composition and a control method for attracting a group of harmful arthropods and protecting plants from the urge of harmful arthropods. A harmful arthropod-attracting composition containing at least 2 members selected from the group consisting of p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, β -farnesene, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-anisidine, methyl m-aminobenzoate, methyl o-methylbenzoate, eugenol, 3-phenylpropionaldehyde, cinnamaldehyde, eucalyptol, squalene, α -hexylcinnamaldehyde, and having a synergistic effect.
In this composition, known attractant components such as ethyl isonicotinate, methyl isonicotinate, propyl isonicotinate, isopropyl isonicotinate, ethyl 2-chloro-isonicotinate, pyridine 4- (1, 3-dioxolan-2-yl), diisopropylisonicotinamide, 4-formylpyridine, methyl 4-pyridone, ethyl 4-pyridone, propyl 4-pyridone, neryl S-methylbutyrate, lavender acetate, folyl alcohol, folal, and folyl acetate may be contained as the harmful arthropod attractant other than the above-mentioned substances.
The patent teaches that the leaf alcohol acetate is only an attractant ingredient and is its attractant effect on arthropods.
Disclosure of Invention
The invention aims to solve the technical problem of providing a new application of the acetic acid leaf alcohol ester in the control of meloidogyne incognita.
In order to solve the technical problem, the invention provides application of acetic acid leaf alcohol ester in preventing and controlling meloidogyne incognita.
As an improvement of the application of the invention: during the growth of the crop, the crop is treated with an open exposure of the leaf alcohol acetate.
As a further improvement of the application of the invention: corn plants (treatment started on day 12 from corn seed planting) were treated with a sustained release unit releasing leaf alcohol acetate at a concentration of 70 ± 10 ng h per plant -1 The treatment time was 1.5. + -. 0.5 hours.
The invention also provides a meloidogyne incognita inhibitor containing the folic acid ester.
The invention also provides a method for preventing and treating meloidogyne incognita by using the acetic acid leaf alcohol ester, which comprises the following steps: during the growth period of crops, the crops are exposed and treated by releasing the folic alcohol acetate by using a slow release device; the method comprises the following specific steps:
corn plants are exposed and treated by releasing the leaf alcohol acetate by using a slow release device on the 12 th day from the planting of the corn seeds, and the treatment concentration is 70 +/-10 ng h for each plant -1 The treatment time was 1.5. + -. 0.5 hours.
A large number of experimental researches show that the exposure treatment of the geraniol acetate can improve the contents of the defense hormones jasmonic acid, jasmonic acid isoleucine conjugate and abscisic acid in plants.
A large number of experimental researches show that the exposure treatment of the acetic acid leaf alcohol ester can obviously inhibit the infection and the propagation of the meloidogyne incognita and obviously reduce the feeding preference of the meloidogyne incognita to host plants.
The specific application form is that after the corn is planted for 12 days, a slow release device containing the acetic acid leaf alcohol ester is exposed to treat corn plants for 1.5 hours, and the treatment concentration is 70ng/h per plant. The corn plants after exposure had fewer nematode infestations and root knots compared to control plants (unexposed). The treatment of the acetic acid leaf alcohol ester can effectively reduce the infection capacity and the reproductive capacity of the meloidogyne incognita. In addition, the treatment of the acetic acid leaf alcohol ester can effectively reduce the feeding preference of the root-knot nematodes on the corn, has an avoidance effect, can reduce the probability of infecting the corn by the root-knot nematodes and has a good control effect.
The invention has the following beneficial effects:
the invention discovers for the first time that the exposure treatment of the leaf alcohol acetate has obvious inhibition effect on the infection and the propagation of the meloidogyne incognita, and the invasion and the propagation of the meloidogyne incognita are obviously inhibited by regulating the up-regulation of the jasmonic acid at the root of the leaf alcohol acetate, and the inhibition mainly comprises the inhibition of the invasion number, the root knot number, the feeding preference and the like of the meloidogyne incognita.
The folic acetate is safe, nontoxic, economic and environment-friendly, and can not bring negative effects on the growth of crops after treatment.
It is emphasized that: the teaching in CN105578879A that folic alcohol acetate is only an attractant ingredient and is an attractant effect on arthropods.
In the invention, the acetic acid leaf alcohol ester is used as a control substance of meloidogyne incognita; meloidogyne incognita belongs to the phylum nematoda, while arthropods belong to the phylum arthropoda, which are essentially different. In addition, CN105578879A only tells that folate acetate is used as a attractant component, whereas in the present invention, folate acetate is used as a root knot nematode infestation inhibitor and feeding deterrent component.
In conclusion, the invention discovers for the first time that the exposure treatment of the phytylene acetate can effectively inhibit the infection and the propagation of the meloidogyne incognita and obviously reduce the feeding preference of the meloidogyne incognita on host plants. The folyl acetate can inhibit the invasion and feeding of meloidogyne incognita by promoting the content of abscisic acid, jasmonic acid and jasmonic acid isoleucine conjugate, which are related hormones of plant root defense. In the application process, the folic acetate is safe, nontoxic, economic and environment-friendly, and can not bring negative influence on the growth of crops after treatment.
Drawings
The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
Fig. 1 is a graph of the treatment of exposure to folate acetate.
FIG. 2 is a graph showing the effect of folate acetate exposure on the number of Meloidogyne incognita entries.
FIG. 3 is a graph showing the effect of leaf alcohol acetate exposure on the number of root knots formed by Meloidogyne incognita.
FIG. 4 is a graph of the effect of folate acetate exposure on feeding preference of Meloidogyne incognita.
FIG. 5 is a graph of the effect of folate acetate exposure on corn root defense-related hormones.
FIG. 6 is a graph of the effect of folate acetate on dry weight of corn after exposure.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. It is within the scope of the present invention to modify or replace methods, steps or conditions of the present invention without departing from the spirit and substance of the present invention.
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1
The embodiment relates to a control experiment of the southern root-knot nematode by the acetic acid leaf alcohol ester.
1. Experimental materials:
The test crop maize B73(Zea mays).
Meloidogyne incognita (Meloidogyne incognita) egg mass.
Folate acetate (cis-3-hexenyl acetate, CAS: 3681-71-8) was purchased from Shanghai Michelin Biotech, Inc.
Glass wool (Glass wool, CAS: 65997-17-3) was purchased from Shanghai Arlatin Biotechnology Ltd.
Blue 9mm vented screw cap (VEAP-5397-09B-100) was purchased from Shanghai spectral Experimental science and technology, Inc.
2. The experimental method comprises the following steps:
preparing a folic acetate ester sustained-release device: winding glass wool into a blue 9mm open-pore screw cap, adding 400 microliters of a pure leaf alcohol acetate solution (purity 98%) to ensure that the release rate of the leaf alcohol acetate is 70ng h 1 。
Planting corn: the corn B73 seeds are sown in cultivation pots (250g soil/pot) and are placed in a greenhouse with the temperature of 26.5 ℃, the humidity of 55% and the carbon dioxide concentration of 456ppm for cultivation and growth for 12 days, and during the cultivation period, the seeds are properly watered according to the dryness and the humidity of sand so as to ensure the normal growth of the corn. A leaf alcohol acetate slow release device is arranged beside each corn plant. After 12 days, corn plants were subjected to a folate acetate exposure treatment, and 1.5 hours after continuous exposure, were immediately inoculated with nematodes. That is, the corn plants were exposed to a slow release device containing leaf alcohol acetate at a concentration of 70ng/h per plant for a period of 1.5 hours.
And (3) nematode infection determination: after the exposure treatment of the folyl acetate, four small holes with the depth of about 2cm are drilled in the range of 1cm-2cm away from the corn base, collected suspension of the two-year-old southern root-knot nematodes are sequentially injected into the small holes by a liquid-transferring gun, then the small holes are filled with a small amount of sand and are watered appropriately according to the dry and wet degree, and 1000-year-old southern root-knot nematodes are inoculated in each pot. Used for measuring the invasion number and the invasion number/corn root system weight, and collecting samples after 3 days of treatment of the inoculated nematodes, thereby obtaining infection indexes of the root-knot nematodes (figure 2); the treated inoculated nematodes used for determining the number of root knot were harvested 10 days later, thereby obtaining a breeding index of root knot nematodes (fig. 3).
Measuring food preference: inserting a 14cm black suction pipe between the corn cultivation pots after the control (the leaf alcohol acetate is not exposed) and the treatment (the leaf alcohol acetate is exposed for 1.5 hours), sequentially inoculating 1500-head two-age southern root-knot nematode suspension liquid in the suction pipe, collecting sand in the cultivation pot after 8 hours, observing and counting under a microscope, and thus obtaining the feeding habit index of the root-knot nematodes after the leaf alcohol acetate treatment (figure 4).
And (3) measuring the phytohormone: exposing with folic acid acetate for 0 hr, 0.5 hr, and 1.5 hr, collecting plant root, grinding, collecting 0.1g of corn root powder, and labeling with ethyl acetate isotope (1ng d) 5 -JA、d 6 -ABA、 13 C 6 -JA-Ile) extracting jasmonic acid, jasmonic acid isoleucine conjugate, abscisic acid from corn root powder and analyzing by UHPLC-MS/MS, thereby obtaining the hormone content index in corn after the treatment of leaf alcohol acetate (fig. 5).
And (3) corn growth index determination: after 1.5 hours of treatment with leaf alcohol acetate exposure, the aerial parts were collected and weighed for dry weight to obtain an indicator of corn growth after leaf alcohol acetate treatment (fig. 6).
3. Results of the experiment
Nematode indices (number of invasion, number of invasion/weight, number of root knots, number of hatchings) of corn roots, hormone levels (jasmonic acid, jasmonic acid-isoleucine conjugate, abscisic acid), and corn growth indices (dry weight) were determined.
2-4, it can be shown that treatment with folate acetate exposure can effectively inhibit the infestation and reproduction of Meloidogyne incognita.
After the exposure treatment of the folyl acetate, compared with a control, the invasion number of the nematodes is remarkably inhibited, the inhibition rate reaches 70% (as shown in figure 2), and the inhibition effect is extremely remarkable. Meanwhile, the number of root knots on the corn root system after the treatment of the exposure to the leaf alcohol acetate was reduced by 33% compared to the control (as shown in fig. 3). As can be seen from the selectivity study, the folic acid acetate has obvious effect of avoiding the nematodes after being exposed, and the feeding preference of the root-knot nematodes to the corn is reduced by 55.67% (as shown in figure 4).
FIG. 5 illustrates that the treatment of leaf acetate exposure can inhibit the infestation and reproduction of Meloidogyne incognita by promoting the synthesis of hormones associated with corn root defense.
As shown in fig. 5, the content of hormones in corn roots was significantly increased after the exposure of the geraniol acetate compared to the control, and after 0.5 hour of exposure, the content of jasmonic acid was increased by 94.25%, the content of jasmonic acid-isoleucine conjugate was increased by 248.53%, and the content of abscisic acid was increased by 40.93%; after 1.5 hours of exposure, the jasmonic acid content is increased by 143.07%, the jasmonic acid isoleucine conjugate content is increased by 220.91%, and the abscisic acid content is increased by 40.98%, which proves that after the corn is treated by the acetic acid leaf alcohol ester, the invasion of the meloidogyne incognita can be inhibited by increasing the hormone at the root of the corn.
As shown in fig. 6, the dry weight of the corn plants after the treatment with leaf alcohol acetate was not affected compared to the untreated plants, indicating that the growth of the crop was not negatively affected by the treatment with leaf alcohol acetate.
Finally, it is also noted that the above-mentioned lists merely illustrate a few specific embodiments of the invention. It is obvious that the invention is not limited to the above embodiments, but that many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the present invention are to be considered within the scope of the invention.

Claims (5)

1. Application of acetic acid leaf alcohol ester in preventing and treating meloidogyne incognita is provided.
2. Use according to claim 1, characterized in that: during the growth of the crop, the crop is treated with an open exposure of the leaf alcohol acetate.
3. Use according to claim 2, characterized in that: the corn plants are treated by using the slow release device to release the acetic acid leaf alcohol ester, and the treatment concentration is 70 +/-10 ng h per plant -1 The treatment time was 1.5. + -. 0.5 hours.
4. A meloidogyne incognita inhibitor contains folic acid ester.
5. The method for controlling meloidogyne incognita by using the leaf alcohol acetate is characterized in that during the growth period of crops, the leaf alcohol acetate is released by a slow release device to expose and treat the crops.
CN202210370540.3A 2022-04-10 2022-04-10 Application of acetic acid phyllyl ester in preventing and treating meloidogyne incognita Active CN114831116B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116897931A (en) * 2023-07-18 2023-10-20 浙江大学 Application of phyllostanol acetate in improving crop resistance and yield and promoting crop growth

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105578879A (en) * 2013-10-02 2016-05-11 协友株式会社 Composition comprising harmful arthropod-attracting substance formed from plant-derived components and analogs thereof

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN105578879A (en) * 2013-10-02 2016-05-11 协友株式会社 Composition comprising harmful arthropod-attracting substance formed from plant-derived components and analogs thereof

Non-Patent Citations (2)

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Title
NADHEM AISSANI: "《Nematicidal Activity of the Volatilome of Eruca sativa on Meloidogyne incognita》", 《J. AGRIC. FOOD CHEM.》 *
翟义乐: "《恶臭假单胞菌 MCCC 1A00316 作用线虫活性物质分离鉴定和其产生的 2-十一烷酮触杀线虫机制研究》", 《华中农业大学博士学位论文》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116897931A (en) * 2023-07-18 2023-10-20 浙江大学 Application of phyllostanol acetate in improving crop resistance and yield and promoting crop growth
CN116897931B (en) * 2023-07-18 2024-03-22 浙江大学 Application of phyllostanol acetate in improving crop resistance and yield and promoting crop growth

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