CN116897931B - Application of phyllostanol acetate in improving crop resistance and yield and promoting crop growth - Google Patents
Application of phyllostanol acetate in improving crop resistance and yield and promoting crop growth Download PDFInfo
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- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 title claims abstract description 57
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- ZNJFBWYDHIGLCU-UHFFFAOYSA-N jasmonic acid Natural products CCC=CCC1C(CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-UHFFFAOYSA-N 0.000 description 1
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- XJPBRODHZKDRCB-UHFFFAOYSA-N trans-alpha-ocimene Natural products CC(=C)CCC=C(C)C=C XJPBRODHZKDRCB-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides application of leaf alcohol acetate in improving the resistance and yield of crops and promoting the growth of the crops, and belongs to the field of continuous cropping obstacle prevention.
Description
Technical Field
The invention belongs to the field of continuous cropping obstacle prevention and treatment, and particularly relates to application of phyllosol acetate in improving crop resistance and yield and promoting crop growth.
Background
Grain production is the basis for guaranteeing stable supply of grains and important agricultural products. Continuous cropping refers to a planting method for continuously planting the same crop in the same field within one year or in the same field in the same year, and the planting method usually causes nutrient imbalance in soil, accumulation of pathogenic bacteria, toxic secondary metabolites and the like, and seriously affects the yield and quality of the crop. Because the cultivated land resources are limited, the method for preventing and treating continuous cropping obstacles is obtained, and has great significance for promoting the growth of crops and improving the resistance and the yield of the crops.
Spodoptera frugiperda of the family spodoptera frugiperda belongs to a common agricultural significant pest with wide host range, predatory and migratory properties in the field. Spodoptera frugiperda (Spodoptera frugiperda), also known as autumn armyworm, is native to tropical and subtropical areas of america and is a major agricultural pest for global early warning by the grain and agriculture organization of united nations. Spodoptera frugiperda has a host range of over 80 plants, but is more favored for field gramineous crops such as corn, wheat, barley, and the like. Farmlands that are compromised by spodoptera frugiperda are more susceptible to insect pests when subjected to continuous cropping. In order to control the damage of these agricultural pests to crops, researchers have conducted many studies of agricultural control, chemical control, biological control, and the like, but chemical control means are still the most widely used in the field. However, these chemical pesticides often present increased resistance to lepidopteran noctuidae pests due to improper use, and the concomitant problems of environmental pollution, high toxicity, and high residue, which pose a threat to food safety and ecological environment. Therefore, it is urgent to find a green plant source reducing agent to perform biological control and guarantee crop yield.
Leaf alcohol Acetate (HAC), a major green leaf volatile (Green Leaf Volatiles, GLV), is a volatile small molecule compound that is produced when plants are subjected to mechanical injury, pathogen infestation, or stress such as feeding by phytophagous animals, and is widely found in various green leaf plants. Studies show that the phyllostanol acetate can be used as a plant early warning signal to enhance the defense reaction of plants. For example, the application of the leaf alcohol acetate in controlling the meloidogyne incognita is disclosed in Chinese patent No. CN114831116A, wherein the leaf alcohol acetate exposure treatment can effectively inhibit the infection and propagation of the meloidogyne incognita, and the feeding preference of the meloidogyne incognita to host plants is obviously reduced. That is, leaf alcohol acetate can inhibit the invasion and feeding of meloidogyne incorporations by promoting the levels of plant root defense-related hormones abscisic acid, jasmonic acid, and jasmonic acid isoleucine conjugate. The Chinese patent No. 109769813B provides a method for trapping and killing tea lesser leafhoppers by pushing and pulling plant repellent and attractant, which is characterized in that the volatile substances cis-3-hexene-1-ol, cis-3-hexenyl acetate, ocimene, methyl salicylate, cis-3-hexenyl butyrate, dodecane, hexadecane and nonanal which are derived from the branches and leaves of the Flemingia macrophylla are proportionally prepared into the attractant with the dosage of 41-51 mg/mL. It is known that phyllitol acetate acts as an attracting component in the attractant.
However, the current research on leaf alcohol acetate is mainly focused on the field of root-knot nematode control and the field of plant protection, and reports on the application of leaf alcohol acetate in the field of continuous cropping obstacle control, crop growth promotion, crop resistance and yield improvement and field application are not yet seen.
Disclosure of Invention
In view of the above, the invention aims to provide the application of the leaf alcohol acetate in improving the resistance and the yield of crops and promoting the growth of the crops, and the invention remarkably inhibits the growth of spodoptera frugiperda by removing sowing second-generation crops after the first-generation crops are exposed and treated by the leaf alcohol acetate, reduces the feeding of the spodoptera frugiperda to the leaves of the crops, improves the resistance of the crops, and simultaneously remarkably improves the yield and the biomass of the crops.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the invention provides an application of phyllostanol acetate in improving crop resistance, which comprises the steps of exposing and treating first-generation crops, then removing the first-generation crops and sowing second-generation crops.
Preferably, the treatment concentration of the leaf alcohol acetate is 50-70 ng/plant, and the treatment time of the leaf alcohol acetate is 0.5-3 h/d.
Preferably, the leaf alcohol acetate exposure treatment is performed when the first generation crop grows to 11-15 d, and the exposure treatment time is 2-5 d.
Preferably, the removal is simultaneous removal of the aerial and subsurface parts of the first generation crop, the removal being performed 1-40 d after the end of the exposure treatment of the first generation crop.
Preferably, the sowing is performed after 1 to 10d of the first crop is removed.
Preferably, the crop plants include corn, wheat, barley and rice.
The invention also provides application of the leaf alcohol acetate in preventing and controlling spodoptera frugiperda insect pests.
Preferably, the controlling comprises controlling spodoptera frugiperda insect pests in continuous cropping.
The invention also provides application of the phyllostanol acetate in improving crop yield.
The invention also provides application of the phyllostanol acetate in promoting crop growth.
Compared with the prior art, the invention has the following beneficial effects:
(1) The invention uses the leaf alcohol acetate to expose and treat the first generation of crops and then remove the first generation of crops, plants the second generation of crops, can obviously enhance the resistance of the crops, inhibit the growth of spodoptera frugiperda, reduce the feeding of spodoptera frugiperda to the leaves of the crops, promote the growth of the crops (such as corn, wheat, barley, rice and the like), and improve the biomass and the yield of the second generation of crops.
(2) The phyllosoyl acetate used in the invention has the characteristics of green, safety, environmental protection, no toxicity and low cost, and can not bring negative effects to the soil and the growth of crops when used for treating crops.
(3) The invention can also adopt agronomic measures such as plant topping and the like to ensure that the crop itself releases the leaf alcohol acetate to achieve the effects of promoting the growth of the crop and improving the resistance of the crop.
Drawings
FIG. 1 is a block diagram of a leaf alcohol acetate sustained release apparatus;
FIG. 2 is a schematic representation of a leaf alcohol acetate exposure treated crop;
FIG. 3 is a graph of biomass change in a second generation maize plant after exposure treatment to a first generation maize plant;
FIG. 4 is a graph of the change in resistance index of a second generation maize plant after exposure treatment to a first generation maize plant;
FIG. 5 is a graph showing the results obtained when corn was subjected to field application testing in Hangzhou, zhejiang (wherein the left side of the bar graph for each group represents the blank control group and the right side represents the phyllostanol acetate treated group);
FIG. 6 is a graph showing the results obtained when three-fold Hainan tests were performed on corn in field applications (wherein the left side of the bar graph for each group represents the blank group and the right side represents the phyllostanol acetate treated group);
FIG. 7 is a graph showing the results obtained when field application tests were performed on corn by Qingyang in Gansu province (wherein the left side of the bar graph of each group represents the blank group, and the right side represents the phyllostanol acetate treated group);
FIG. 8 is a morphology of kernels of randomly selected second generation maize plants when a field application test is performed on maize by Qingyang in Gansu;
FIG. 9 is a plot of the biomass change of a second generation wheat plant measured after exposure of corn;
FIG. 10 is a graph showing the change in resistance index of second generation wheat plants measured after exposure treatment of corn;
FIG. 11 is a graph of the biomass change of a second generation barley plant measured after exposure treatment of corn;
FIG. 12 is a graph showing the change in resistance index of second generation barley plants measured after exposure treatment of maize;
FIG. 13 is a graph showing the change in biomass of second generation rice plants measured after exposure of corn;
FIG. 14 is a graph showing the change in resistance index of second-generation rice plants measured after exposure treatment of corn.
Detailed Description
The invention provides an application of phyllostanol acetate in improving crop resistance, which comprises the steps of exposing and treating first-generation crops, then removing the first-generation crops and sowing second-generation crops.
In the present invention, the treatment concentration of the leaf alcohol acetate is preferably 50 to 70 ng/strain, more preferably 55 to 65 ng/strain, still more preferably 60 ng/strain; the treatment time of the leaf alcohol acetate is preferably 0.5 to 3 hours/d, more preferably 1 to 2.5 hours/d, still more preferably 1.5 to 2 hours/d; the leaf alcohol acetate exposure treatment is preferably performed when the first-generation crop grows to 11 to 15d, more preferably performed when the first-generation crop grows to 12 to 14d, and still more preferably performed when the first-generation crop grows to 13 d; the exposure time is preferably 2 to 5 days, more preferably 3 to 4 days, still more preferably 2.5 days; the removal is preferably performed by simultaneously removing the aerial part and the underground part of the first-generation crop, preferably performed 1 to 40d after the end of the exposure treatment of the first-generation crop, more preferably performed 2 to 30d after the end of the exposure treatment of the first-generation crop, still more preferably performed 3 to 20d after the end of the exposure treatment of the first-generation crop, still more preferably performed 4 to 10d after the end of the exposure treatment of the first-generation crop; the sowing is preferably performed after 1-10 d of the first-generation crop is removed, more preferably performed after 3-7 d of the first-generation crop is removed, still more preferably performed after 4-6 d of the first-generation crop is removed, still more preferably performed after 5d of the first-generation crop is removed; the crops preferably include maize, wheat, barley and rice.
In the invention, the exposure treatment is preferably carried out by adopting a leaf alcohol acetate slow-release device, and the preparation method of the leaf alcohol acetate slow-release device preferably comprises the steps of placing glass wool in a bottle, adding the leaf alcohol acetate, covering a bottle cap with a small hole, and inserting a trace capillary tube; further preferably, the method comprises placing glass wool in a screw-thread sample injection bottle, adding phyllitol acetate, covering with a threaded cover with an opening, and inserting a microcapillary.
The invention also provides application of the leaf alcohol acetate in preventing and controlling spodoptera frugiperda insect pests.
In the present invention, the control preferably includes control of spodoptera frugiperda insect pests in continuous cropping.
The invention also provides application of the phyllostanol acetate in improving crop yield.
The invention also provides application of the phyllostanol acetate in promoting crop growth.
In another embodiment of the invention, the leaf alcohol acetate may also be released by the first generation crop itself by topping the plant.
In the present invention, the plant topping is another method instead of exposure treatment, preferably by pruning the leaves on top of the first generation crop plants grown to the jointing stage. In the invention, the blade preferably cuts out 3 to 5 pieces, more preferably cuts out 4 pieces; the plant topping also comprises the steps of removing the first generation crop and sowing the second generation crop.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1 preparation of a device for sustained release of phyllitol acetate
Glass wool (glass wool, CAS:65997-17-3, available from Shanghai Abat Biotechnology Co., ltd.) was wound into a moderately elastic pellet and placed in a 2mL screw-type sample bottle (product number: VAAP-320097M-1232, available from Shanghai England Biotechnology Co., ltd.), 100. Mu.L of 98% strength in an acetic acid leaf alcohol ester solution (cis-3-hexenylacetate, CAS:3681-71-8, available from Shanghai Milin Biotechnology Co., ltd.) was added, and a disposable microcapillary (product number: 13013, 2. Mu.L, 0.73mm in outside diameter, 0.28mm in Germany, 1cm in length, available from Heman laboratory Co.) was inserted onto a mating 9mm open-cell screw cap (product number: VEAP-5397-09B-100, available from Shanghai England Biotechnology Co., ltd.) and wrapped with a paper closure film to release the acetic acid leaf alcohol ester at 60ng/h.
Example 2
Sowing corn seeds into black cultivation pot (300 g soil/pot) at 25deg.C with humidity of 50% and CO 2 Carrying out illumination treatment in a greenhouse with the concentration of 500ppm, and carrying out illumination for 14 hours every day, wherein 10mL of each corn plant is watered in the morning and evening every day, so that the normal growth of the corn is ensured; when corn grows to 12d, 8:30 a.m. each day, placing a leaf alcohol acetate slow-release device with a release rate of 60ng/h beside each corn, and exposing for 1.5h, and continuously exposing for 3d in this way; the aerial and underground parts of the first-generation corn were simultaneously removed 1 day after the end of the exposure treatment, the second-generation corn was sown on the day when the first-generation corn was removed, and the crops were placed in the same greenhouse environment as the above-mentioned first-generation crops for cultivation. Meanwhile, a blank control group is arranged, wherein the blank control group is the treatment of replacing the leaf alcohol acetate slow-release device with the same slow-release device without adding the leaf alcohol acetate solution.
When the second generation corn grows to 20d, the aerial parts of the corn plants are collected and put into kraft paper bags, and are dried in an electrothermal blowing drying oven at 60 ℃ for 3 days, then the dry weight is weighed, and data are recorded, and the result is shown in figure 3.
As can be seen from fig. 3, the treatment with phyllosol acetate significantly promoted the growth of the second-generation crop, showing higher biomass than the control group.
Example 3
After corn plants were treated according to the method of example 2, when the second generation corn was grown to 20d, spodoptera frugiperda larvae of second age having an initial weight of between 1.8 and 2.6mg (initial weight: 2.2.+ -. 0.4mg, purchased from Henan Jiyuan white cloud industry Co., ltd.) were selected, pre-starved for 2 hours, and after pre-starvation treatment, larvae were inoculated onto the third fully developed leaf according to the positional relationship of leaf and larva as shown in FIG. 4, and feeding was simulated by insect pests.
Within three days after insect inoculation, leaf sections fed by spodoptera frugiperda were harvested every 24h interval, attached to A4 paper, and leaf loss areas were calculated using the software Digimizer (version 5.6.0) after color scanning. After 72h inoculation, spodoptera frugiperda larvae were collected and weighed, the final weight was recorded and the larval growth rate was calculated according to formula (1). The results are shown in FIG. 4.
As can be seen from fig. 4, the mode of removing the sown second-generation crop after the first-generation crop is treated with the leaf alcohol acetate reduces the feeding area of spodoptera frugiperda on the leaves of the second-generation crop and inhibits the growth of spodoptera frugiperda larvae compared with the control group, which indicates that the leaf alcohol acetate treatment can improve the resistance of the second-generation crop.
Example 4
The field application tests were carried out in three different climates, different soil types, respectively, in Hangzhou Zhejiang, hainan three-layer, gansu Qingyang during 2021-2022.
10 6 m-6 m cells were divided in the selected field, a buffer zone of 1.5m interval was set between each cell, a control group and a leaf alcohol acetate treatment group were set at random intervals, the first generation crop was treated and the second generation crop was sown according to the method of example 2, and when the second generation crop was grown to 20d, the second generation crop was evaluated according to the methods of example 2 and example 3.
The evaluation method for the resistance of the second-generation crops is to count the number of insects on the field corn in a plurality of time points of 18-60 days of the growing period of the second-generation crops; the yield evaluation method comprises the following steps: after the corns are fully mature, corn cobs in the same district are harvested, threshed and mixed uniformly, and thousand grain weight is counted. The results are shown in FIGS. 5-7.
From fig. 5-7, compared with the control, the biomass and the yield of the second-generation crop treated by the leaf alcohol acetate are obviously improved, and the second-generation crop can obviously inhibit the growth of spodoptera frugiperda and improve the crop resistance.
Example 5
The second generation maize plants planted in example 2 were replaced with wheat, barley and rice, respectively, and the remaining treatment and measurement methods were the same as in example 2. And the resistance index of the second generation wheat, barley and rice was measured as in example 3. The effect of the treatment with leaf alcohol acetate exposure on the second generation crop at rotation was investigated.
The results of the measured growth performance (e.g., aboveground biomass) and resistance index (e.g., larval weight gain rate, leaf loss area) of the second generation wheat, barley and rice are shown in fig. 9 to 14.
From fig. 9-14, it can be seen that the exposure treatment can also promote the growth of wheat, barley and rice, reduce the rate of weight gain and the feeding area of spodoptera frugiperda, and increase the resistance of wheat, barley and rice to spodoptera frugiperda.
Example 6
The exposure treatment in example 2 was replaced with a plant topping treatment, and the rest was exactly the same as in example 2.
The plant topping treatment method is to trim 3 leaves on the top of the first generation crop plant growing to the jointing period.
In addition, the results of each of the above examples were analyzed by variance to calculate whether there was a significant difference between the blank and the phyllitol acetate treated groups. In fig. 3-14 "×" represents P <0.05; "x" represents P <0.01; ", represents P <0.001.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (1)
1. Use of leaf alcohol acetate for increasing the resistance of a crop to spodoptera frugiperda, characterized in that a leaf alcohol acetate slow release device is placed beside the crop to expose the first generation of said crop, and then the first generation of said crop is removed and the second generation of said crop is sown;
the crops are corn, wheat, barley and rice;
the leaf alcohol acetate exposure treatment is carried out when the first generation of crops grow to 11-15 d, and the exposure treatment time is 2-5 d;
the treatment concentration of the leaf alcohol acetate is 50-70 ng/plant, and the treatment time of the leaf alcohol acetate is 0.5-3 h/d;
the removing is to simultaneously remove the aerial part and the underground part of the first-generation crop, wherein the removing is performed 1-40 d after the exposing treatment of the first-generation crop is finished;
the sowing is performed after 1-10 d of the first generation crops are removed.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110074107A (en) * | 2019-05-14 | 2019-08-02 | 中国农业科学院植物保护研究所 | A kind of Spodopterafrugiperda attractant composition and its application |
| CN113331201A (en) * | 2021-06-03 | 2021-09-03 | 中国农业科学院植物保护研究所 | Attractant composition for preventing and treating spodoptera frugiperda and application thereof |
| CN114451407A (en) * | 2021-12-03 | 2022-05-10 | 福建省农业科学院植物保护研究所 | Sex attractant for Spodoptera frugiperda, and preparation method and application thereof |
| CN114831116A (en) * | 2022-04-10 | 2022-08-02 | 浙江大学 | Application of acetic acid leaf alcohol ester in preventing and treating meloidogyne incognita |
| CN115769818A (en) * | 2023-01-30 | 2023-03-10 | 华南农业大学 | Application of (cis) -3-hexenol acetate in inducing spodoptera frugiperda to lay eggs and serving as spodoptera frugiperda attractant |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074107A (en) * | 2019-05-14 | 2019-08-02 | 中国农业科学院植物保护研究所 | A kind of Spodopterafrugiperda attractant composition and its application |
| CN113331201A (en) * | 2021-06-03 | 2021-09-03 | 中国农业科学院植物保护研究所 | Attractant composition for preventing and treating spodoptera frugiperda and application thereof |
| CN114451407A (en) * | 2021-12-03 | 2022-05-10 | 福建省农业科学院植物保护研究所 | Sex attractant for Spodoptera frugiperda, and preparation method and application thereof |
| CN114831116A (en) * | 2022-04-10 | 2022-08-02 | 浙江大学 | Application of acetic acid leaf alcohol ester in preventing and treating meloidogyne incognita |
| CN115769818A (en) * | 2023-01-30 | 2023-03-10 | 华南农业大学 | Application of (cis) -3-hexenol acetate in inducing spodoptera frugiperda to lay eggs and serving as spodoptera frugiperda attractant |
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