CN114885902A - Breeding method of Spodoptera frugiperda - Google Patents
Breeding method of Spodoptera frugiperda Download PDFInfo
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- CN114885902A CN114885902A CN202210598174.7A CN202210598174A CN114885902A CN 114885902 A CN114885902 A CN 114885902A CN 202210598174 A CN202210598174 A CN 202210598174A CN 114885902 A CN114885902 A CN 114885902A
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- 238000009395 breeding Methods 0.000 title claims abstract description 27
- 241000256251 Spodoptera frugiperda Species 0.000 title claims abstract description 25
- 230000001488 breeding effect Effects 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 21
- 230000032669 eclosion Effects 0.000 claims abstract description 18
- 230000019617 pupation Effects 0.000 claims abstract description 14
- 230000017448 oviposition Effects 0.000 claims abstract description 5
- 239000002689 soil Substances 0.000 claims description 32
- 235000013601 eggs Nutrition 0.000 claims description 26
- 241000382353 Pupa Species 0.000 claims description 23
- 240000008042 Zea mays Species 0.000 claims description 23
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 23
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 23
- 235000005822 corn Nutrition 0.000 claims description 23
- 241001057636 Dracaena deremensis Species 0.000 claims description 18
- 239000004677 Nylon Substances 0.000 claims description 10
- 229920001778 nylon Polymers 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 229920000742 Cotton Polymers 0.000 claims description 9
- 235000012907 honey Nutrition 0.000 claims description 9
- 241000238631 Hexapoda Species 0.000 claims description 8
- 238000011534 incubation Methods 0.000 claims description 8
- 230000013011 mating Effects 0.000 claims description 6
- 230000001418 larval effect Effects 0.000 claims description 4
- 208000003643 Callosities Diseases 0.000 claims description 3
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 3
- 230000012447 hatching Effects 0.000 abstract description 5
- 238000007796 conventional method Methods 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000002265 prevention Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000003699 hair surface Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
A method for breeding Spodoptera frugiperda. Mainly solves the problems of low egg laying and emergence rate and high death rate of the existing breeding method of spodoptera frugiperda. The invention provides a method for raising Spodoptera frugiperda, which is higher than the conventional method in egg hatching rate, larva survival rate, pupation rate and eclosion rate, greatly reduces the steps required for operation, saves the time of raising personnel and reduces the labor cost.
Description
Technical Field
The invention relates to the technical field of agricultural biology, in particular to a method for raising Spodoptera frugiperda.
Background
Spodoptera frugiperda (Smith), also known as fall armyworm, belongs to the Lepidoptera family of Spodoptera frugiperda, is native to the tropical and subtropical regions of the America, is a pest that seriously threatens agricultural production, and has recently invaded Africa and Asia in succession. China finds that the safety of agriculture and grain production in 2019 for the first time in China is seriously threatened. The indoor feeding of the insect is the basis for developing the research work of biological characteristics and ecology, provides test materials for the research of prevention and control technologies such as prevention and control measures combining later-stage biological prevention and control, physical and chemical induction and control and chemical prevention and control, prediction and early warning and the like, and provides theoretical support for the research of emergency prevention and control, comprehensive treatment and the like of the insect.
Compared with the natural environment, the common indoor feeding method is easy to have the problems of spawning, low eclosion rate, high mortality rate and the like.
Disclosure of Invention
In order to overcome the defects of the background art, the invention provides a method for raising spodoptera frugiperda, which mainly solves the problems of low egg laying rate, low emergence rate and high death rate of the conventional method for raising spodoptera frugiperda.
The technical scheme adopted by the invention is as follows:
a method for raising Spodoptera frugiperda comprises the following steps:
step one, breeding the high-age larvae to a pupation stage,
independently placing the high-age larvae caught in the field or bred and matured by the low-age larvae into a breeding bag containing soil, placing 4-7 cm of sterilized soil at the bottom of the breeding bag, and putting artificial feed, wherein the artificial feed usually can penetrate into the soil to pupate in 10-20 days;
step two, from pupation stage to eclosion into imagoes,
after the larvae are observed to penetrate into the soil and pupate, the nylon bag is moved into a gauze covered by the potted corn, and the corns in the gauze move after the pupae eclosion;
step three, from the adult stage to the oviposition stage,
putting 8-12 bags of pupa into each potted corn; after the imagoes are eclosized, absorbent cotton which absorbs 5-15% of honey water can be hung in the gauze; after mating, the insects lay eggs on the back of the leaves; 1-2 eggs are cut and left for continuous feeding;
step four, from the egg stage to the incubation larval stage
And (3) directly eating fresh corn plants after the reserved egg blocks are naturally incubated, and immediately moving the fresh corn plants into a feed bag for independent feeding after the corn plants grow to 4 th instar by controlling the pupa moving-in time, namely the step one.
The high-age larvae in the step one are larvae of 4-6 years old.
The feeding bag in the step one is a nylon feeding bag, the soil containing part is provided with a support (1), and the top of the feeding bag is folded and hung through a drawstring.
In the fourth step, by controlling the pupa immigration time, the growth period of the potted corn is ensured to be between the later stage of a large bell mouth and the heading stage during larva incubation, and enough growth vigor and growth time can be ensured for 1-3 instar larvae to eat.
The height of the soil in the first step is 5 cm.
The bottom of the feeding bag in the step one is of a structure capable of being opened and closed.
In the third step, absorbent cotton which absorbs 10 percent of honey water is hung.
The invention has the beneficial effects that: the invention provides a method for raising Spodoptera frugiperda, which is higher than the conventional method in egg hatching rate, larva survival rate, pupation rate and eclosion rate, greatly reduces the steps required for operation, saves the time of raising personnel and reduces the labor cost.
Drawings
FIG. 1 is a schematic flow chart of an embodiment of the present invention.
Detailed Description
The invention will be further described with reference to the accompanying drawings in which: as shown in the figure, the method for raising Spodoptera frugiperda comprises the following steps:
step one, breeding the high-age larvae to a pupation stage,
independently placing the high-age larvae caught in the field or bred and matured by the low-age larvae into a breeding bag containing soil, placing 4-7 cm of sterilized soil at the bottom of the breeding bag, and putting artificial feed, wherein the artificial feed usually can penetrate into the soil to pupate in 10-20 days; the larval of high age is 4-6 years old, 1, raise the larval of 4 years old and above alone, can avoid killing each other. 2. The soil layer can simulate the natural habitat, the pupa forming and eclosion success rate can be increased 3, the glass or other hard materials are usually used in the breeding method commonly used in the laboratory, but the breeding in the container has the defects that the excrement is mixed with the feed, and the dew condensation water is generated to cause the feed to go moldy, so that the feed is deteriorated, the death rate of the larvae is high, and the health condition of the subsequent development stage is influenced even if the larvae survive.
Step two, from pupation stage to eclosion into imagoes,
after the larvae are observed to penetrate into the soil and pupate, the nylon bag is moved into a gauze covered by the potted corn, and the corns in the gauze move after the pupae eclosion; 1. the pupa forming process is reduced to a natural state, and the pupation and eclosion success rate is improved. 2. The movement is convenient, and tweezers or direct hands can be used when the worm pupae in the culture dish are moved by a common method, so that the risk of damage is caused, and the probability of infection is increased. Meanwhile, the independent split charging feeding also increases the moving convenience and improves the operation efficiency. The labor cost is saved.
Step three, from the adult stage to the oviposition stage,
putting 8-12 bags of pupa into each potted corn; after the imagoes are eclosized, absorbent cotton which absorbs 5-15% of honey water can be hung in the gauze; after mating, the insects lay eggs on the back of the leaves; 1-2 eggs are cut and left for continuous feeding; 1. the spawning environment is restored to the original state, and the spawning and hatching rate is improved. 2. The link of placing the filter paper for spawning is reduced. The problem that the filter paper is not laid on the filter paper after being placed can also be avoided.
Step four, from the egg stage to the larva hatching stage
And (3) directly eating fresh corn plants after the reserved egg blocks are naturally incubated, and immediately moving the fresh corn plants into a feed bag for independent feeding after the corn plants grow to 4 th instar by controlling the pupa moving-in time, namely the step one. The corn leaves are used for feeding instead of artificial feed for low larvae, so that not only is one step of labor saved, but also the original habitat is restored, and meanwhile, the problems of pollution, mildew and the like caused by the artificial feed are avoided.
The high-age larvae in the step one are larvae of 4-6 years old.
The feeding bag in the step one is a nylon feeding bag, the soil containing part is provided with a support, and the top of the feeding bag is folded and hung through a drawstring. The support can be a support plate for fixing the soil part.
In the fourth step, by controlling the pupa immigration time, the growth period of the potted corn is ensured to be between the later stage of a large bell mouth and the heading stage during larva incubation, and enough growth vigor and growth time can be ensured for 1-3 instar larvae to eat.
The height of the soil in the first step is 5 cm.
The bottom of the feeding bag in the step one is of a structure capable of being opened and closed. The bottom of the feeding belt can be provided with a structure of a magic belt, the front and back surfaces are hook surfaces, the left and right surfaces are hair surfaces, and the nylon bag can be vertically seated at the bottom of the flowerpot after being adhered to each other. After pupae eclosion, the corn in the net moves.
In the third step, absorbent cotton which absorbs 10 percent of honey water is hung.
Example one
A method for raising Spodoptera frugiperda comprises the following steps: step one, breeding the high-age larvae to a pupation stage, independently placing the high-age larvae caught in the field or bred and matured by the low-age larvae into a breeding bag containing soil, placing 4cm of sterilized soil at the bottom of the breeding bag, and putting artificial feed into the breeding bag, wherein the high-age larvae usually enter the soil to pupate in 12 days; step two, when the pupa stage is reached to eclosion and the pupa is formed, after the fact that the larvae are drilled into soil and pupate is observed, the nylon bag is moved into a gauze covered by potted corn, and the corn in the gauze moves after the pupa is eclosion; step three, from an adult stage to a spawning stage, putting 8 bags of pupae into each potted corn; after the imagoes are eclosized, absorbent cotton which absorbs 5% of honey water can be hung in the gauze; after mating, the insects lay eggs on the back of the leaves; 1-2 eggs are cut and left for continuous feeding; and step four, from the egg stage to the larva incubation stage, directly eating fresh corn plants after the reserved egg blocks are naturally incubated, and immediately moving the corn plants into a feed bag for independent feeding when the corn plants grow to 4 th instar by controlling the pupa moving-in time, namely the step one.
Example two
A method for raising Spodoptera frugiperda comprises the following steps: step one, breeding the high-age larvae to a pupation stage, independently placing the high-age larvae caught in the field or bred and matured by the low-age larvae into a breeding bag containing soil, placing 5cm of sterilized soil at the bottom of the breeding bag, and putting artificial feed into the breeding bag, wherein the high-age larvae usually can dig into the soil to pupate in 15 days; step two, when the pupa stage is reached to eclosion and the pupa is formed, after the fact that the larvae are drilled into soil and pupate is observed, the nylon bag is moved into a gauze covered by potted corn, and the corn in the gauze moves after the pupa is eclosion; step three, from an adult stage to a spawning stage, putting 10 bags of pupae into each potted corn; after the imagoes are eclosized, absorbent cotton capable of absorbing 7% of honey water can be hung in the gauze; after mating, the insects lay eggs on the back of the leaves; 1-2 eggs are cut and left for continuous feeding; and step four, from the egg stage to the larva incubation stage, directly eating fresh corn plants after the reserved egg blocks are naturally incubated, and immediately moving the corn plants into a feed bag for independent feeding when the corn plants grow to 4 th instar by controlling the pupa moving-in time, namely the step one.
EXAMPLE III
A method for raising Spodoptera frugiperda comprises the following steps: step one, breeding the high-age larvae to a pupation stage, independently placing the high-age larvae caught in the field or bred and matured by the low-age larvae into a breeding bag containing soil, placing 8cm of sterilized soil at the bottom of the breeding bag, and putting artificial feed into the breeding bag, wherein the high-age larvae usually enter the soil to pupate in 15 days; step two, when the pupa stage is reached to eclosion and the pupa is formed, after the fact that the larvae are drilled into soil and pupate is observed, the nylon bag is moved into a gauze covered by potted corn, and the corn in the gauze moves after the pupa is eclosion; step three, from an adult stage to a spawning stage, putting 12 bags of pupae into each potted corn; after the imagoes are eclosized, absorbent cotton which absorbs 10% of honey water can be hung in the gauze; after mating, the insects lay eggs on the back of the leaves; 1-2 eggs are cut and left for continuous feeding; and step four, from the egg stage to the larva incubation stage, directly eating fresh corn plants after the reserved egg blocks are naturally incubated, and immediately moving the corn plants into a feed bag for independent feeding when the corn plants grow to 4 th instar by controlling the pupa moving-in time, namely the step one.
The Spodoptera frugiperda bred by the first, second and third embodiments has an egg hatching rate of over 95%, a larva survival rate of over 63%, a pupation rate of over 94%, an emergence rate of over 88%, which is higher than that of the conventional method by about 90%, a larva survival rate of about 29%, a pupation rate of about 70%, and an emergence rate of about 70%
The examples are illustrative and are intended to be illustrative of the invention and should not be construed as limiting the invention. The examples should not be construed as limiting the present invention, but any modifications made based on the spirit of the present invention should be within the scope of protection of the present invention.
Claims (7)
1. A raising method of Spodoptera frugiperda is characterized by comprising the following steps: the method comprises the following steps:
step one, breeding the high-age larvae to a pupation stage,
independently placing the high-age larvae caught in the field or bred and matured by the low-age larvae into a breeding bag containing soil, placing 4-7 cm of sterilized soil at the bottom of the breeding bag, and putting artificial feed, wherein the artificial feed usually can penetrate into the soil to pupate in 10-20 days;
step two, from pupation stage to eclosion into imagoes,
after the larvae are observed to penetrate into the soil and pupate, the nylon bag is moved into a gauze covered by the potted corn, and the corns in the gauze move after the pupae eclosion;
step three, from the adult stage to the oviposition stage,
putting 8-12 bags of pupa into each potted corn; after the imagoes are eclosized, absorbent cotton which absorbs 5-15% of honey water can be hung in the gauze; after mating, the insects lay eggs on the back of the leaves; 1-2 eggs are cut and left for continuous feeding;
step four, from the egg stage to the incubation larval stage
And (3) directly eating fresh corn plants after the reserved egg blocks are naturally incubated, and immediately moving the fresh corn plants into a feed bag for independent feeding after the corn plants grow to 4 th instar by controlling the pupa moving-in time, namely the step one.
2. The method for raising spodoptera frugiperda according to claim 1, wherein: the high-age larvae in the step one are larvae of 4-6 years old.
3. The method for raising spodoptera frugiperda according to claim 1, wherein: the feeding bag in the step one is a nylon feeding bag, the soil containing part is provided with a support, and the top of the feeding bag is folded and hung through a drawstring.
4. The method for raising spodoptera frugiperda according to claim 1, wherein: in the fourth step, by controlling the pupa immigration time, the growth period of the potted corn is ensured to be between the later stage of a large bell mouth and the heading stage during larva incubation, and enough growth vigor and growth time can be ensured for 1-3 instar larvae to eat.
5. The method for raising spodoptera frugiperda according to claim 1, wherein: the height of the soil in the first step is 5 cm.
6. The method for raising spodoptera frugiperda according to claim 1, wherein: the bottom of the feeding bag in the step one is of a structure capable of being opened and closed.
7. The method for raising spodoptera frugiperda according to claim 1, wherein: in the third step, absorbent cotton which absorbs 10 percent of honey water is hung.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210598174.7A CN114885902A (en) | 2022-05-30 | 2022-05-30 | Breeding method of Spodoptera frugiperda |
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| CN202210598174.7A CN114885902A (en) | 2022-05-30 | 2022-05-30 | Breeding method of Spodoptera frugiperda |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014061853A1 (en) * | 2012-10-18 | 2014-04-24 | 경기도 | Method for artificially breeding illeis koebelei |
| CN207639504U (en) * | 2017-08-03 | 2018-07-24 | 陈凌云 | Novel plants insect inoculation bag |
| CN110199743A (en) * | 2019-07-12 | 2019-09-06 | 浙江绿神天敌生物技术有限公司 | A kind of control method of Spodopterafrugiperda |
| CN111758672A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots |
| CN112205358A (en) * | 2020-09-28 | 2021-01-12 | 云南农业大学 | Indoor population propagation method for spodoptera frugiperda |
| CN112772573A (en) * | 2021-01-27 | 2021-05-11 | 广西壮族自治区农业科学院植物保护研究所 | Indoor artificial breeding method for acrossodes amabilis |
| CN113749058A (en) * | 2021-10-21 | 2021-12-07 | 贵州大学 | Indoor device for efficiently breeding spodoptera frugiperda population |
-
2022
- 2022-05-30 CN CN202210598174.7A patent/CN114885902A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014061853A1 (en) * | 2012-10-18 | 2014-04-24 | 경기도 | Method for artificially breeding illeis koebelei |
| CN207639504U (en) * | 2017-08-03 | 2018-07-24 | 陈凌云 | Novel plants insect inoculation bag |
| CN110199743A (en) * | 2019-07-12 | 2019-09-06 | 浙江绿神天敌生物技术有限公司 | A kind of control method of Spodopterafrugiperda |
| CN111758672A (en) * | 2020-07-16 | 2020-10-13 | 中国热带农业科学院环境与植物保护研究所 | Group breeding method for feeding spodoptera frugiperda larvae with young corn shoots |
| CN112205358A (en) * | 2020-09-28 | 2021-01-12 | 云南农业大学 | Indoor population propagation method for spodoptera frugiperda |
| CN112772573A (en) * | 2021-01-27 | 2021-05-11 | 广西壮族自治区农业科学院植物保护研究所 | Indoor artificial breeding method for acrossodes amabilis |
| CN113749058A (en) * | 2021-10-21 | 2021-12-07 | 贵州大学 | Indoor device for efficiently breeding spodoptera frugiperda population |
Non-Patent Citations (1)
| Title |
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| 张红梅 等: "卵和蛹短时高温对草地贪夜蛾生长发育的影响", 《植物保护学报》 * |
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Application publication date: 20220812 |