CN104472284B - In Nicotiana tabacum L., interplant vegetable breed the method that aphidius gifuensis is prevented eliminating aphis - Google Patents
In Nicotiana tabacum L., interplant vegetable breed the method that aphidius gifuensis is prevented eliminating aphis Download PDFInfo
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- CN104472284B CN104472284B CN201410750004.1A CN201410750004A CN104472284B CN 104472284 B CN104472284 B CN 104472284B CN 201410750004 A CN201410750004 A CN 201410750004A CN 104472284 B CN104472284 B CN 104472284B
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- 235000013311 vegetables Nutrition 0.000 title claims abstract description 85
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 47
- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 26
- 244000061176 Nicotiana tabacum Species 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 14
- 241001600407 Aphis <genus> Species 0.000 title claims abstract description 10
- 241001124076 Aphididae Species 0.000 claims abstract description 215
- 241000196324 Embryophyta Species 0.000 claims abstract description 68
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 45
- 230000003071 parasitic effect Effects 0.000 claims abstract description 39
- 241000256844 Apis mellifera Species 0.000 claims abstract description 37
- 241000208125 Nicotiana Species 0.000 claims abstract description 26
- 239000000575 pesticide Substances 0.000 claims abstract description 6
- 238000011081 inoculation Methods 0.000 claims description 16
- 238000009395 breeding Methods 0.000 claims description 15
- 230000001488 breeding effect Effects 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241000219193 Brassicaceae Species 0.000 claims description 5
- 239000003337 fertilizer Substances 0.000 claims description 4
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 4
- 230000024241 parasitism Effects 0.000 claims description 4
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 claims description 4
- 239000013589 supplement Substances 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 2
- 239000000126 substance Substances 0.000 abstract description 6
- 230000002085 persistent effect Effects 0.000 abstract description 3
- 230000005923 long-lasting effect Effects 0.000 abstract description 2
- 241000721621 Myzus persicae Species 0.000 abstract 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 17
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 13
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 13
- 241000700605 Viruses Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001468872 Fumagospora capnodioides Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
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- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
nullA kind of in Nicotiana tabacum L. interplant vegetable breed the method that aphidius gifuensis is prevented eliminating aphis，When tobacco seedlings is transplanted in the middle of the cigarette strain of vega the brassicaceous vegetable Seedling of 20 40 strain parent aphids in every mu of interplanting，Before starting in cigarette strain aphid evil occurs，It is inoculated into aphid kind room is cultivated the parasitic aphid obtained in vega on the brassicaceous vegetable Seedling of interplanting，Said method is used directly to carry out the numerous aphid of nature and numerous honeybee on the vegetable plant strain of interplanting in vega，Helping the aphidius gifuensis in vega long lasting for ground strengthens defences controls Nicotiana tabacum L. aphid evil，The amount of application of tobacco planting link chemical pesticide is greatly reduced，Improve the safety of Nicotiana tabacum L.，The present invention is long to the preventing and treating persistent period of tobacco aphid、Effective，Required numerous honeybee small scale，Low cost，Breed the aphidius gifuensis resistance obtained，High to the cigarette strain anti-aphid efficiency in vega，Need not artificially collect and discharge aphidius gifuensis，Operation is simple，Save labor.
Description
Technical field
The invention belongs to the technical field of biological control of agriculture and forestry injurious insect, particularly to a kind of in Nicotiana tabacum L. interplant vegetable breed the method that aphidius gifuensis is prevented eliminating aphis.
Background technology
Cigarette aphid is one of primary pest during leaf tobacco production, plant nutrition is caused to deteriorate by sucking the nutrient substance of cigarette strain, affect normal plants and growth, the Excreta honeydew of cigarette aphid remains in the blade polluting plant in cigarette strain simultaneously, can affect plant and normally carry out photosynthesis, induce sooty mould, cigarette aphid is the communication media of multiple virus, transmitted virus, causes the viroses of plant popular, has a strong impact on quality of tobacco, yield.
The method preventing and treating cigarette aphid has cultural control, chemical control, physical control and natural enemy preventing and treating, and people rely primarily on and use chemical insecticide to carry out chemical control for a long time, and the Drug resistance of cigarette aphid constantly strengthens, and has a strong impact on the safety of Nicotiana tabacum L..
Aphidius gifuensis is a kind of advantage parasitic wasp of cigarette aphid, this honeybee kind is widely distributed, high to cigarette aphid parasitic rate, it is applied in Nicotiana tabacum L., vegetable crop anti-eliminating aphis, prevention effect is notable, and low currently with aphidius gifuensis preventing and treating technology length breeding cycle of cigarette aphid, efficiency, the effectively persistent period of preventing and treating aphid evil is short, collecting and release aphidius gifuensis is taken a lot of work arduously, cost is high.
Summary of the invention
The aphid on Biological control tobacco plant is carried out for utilizing aphidius gifuensis, prior art exists and builds numerous honeybee facility cost height, it is accomplished manually collection and the release of aphidius gifuensis, operation is loaded down with trivial details, workload is big, and breeding cycle is long, inefficient problem, there is provided a kind of in Nicotiana tabacum L. interplant vegetable breed the method that aphidius gifuensis is prevented eliminating aphis, the enforcement step of the method is:
(1) aphid kind is raised
The brassicaceous vegetable Seedling of selection aphid hobby, as the cultivation plant of aphid, the annual 1-4 month, the brassicaceous vegetable Seedling of the indoor cultivation in batches parent aphid of the aphid kind in booth, every batch of culturing vegetable Seedling 20-100 strain, was cultivated a collection of every 20 days；After aphid kind first vegetable seedling indoor is cultivated 20 days, gathering white, yellow green or green type cigarette aphid to field, the cigarette aphid collected in field is inoculated in aphid kind room on the plant of first vegetable seedling cultivated, every strain vegetable seedling plant connects aphid 30；Cigarette aphid growth and breeding after 20 days on the plant of vegetable seedling, the effective vegetable seedling of numerous aphid is selected as first aphid kind strain from the vegetable seedling plant that first is cultivated, the aphid selected in first aphid kind strain obtained is inoculated on the vegetable seedling plant that second batch is cultivated, every strain vegetable seedling plant inoculates aphid 30；Cigarette aphid growth and breeding after 20 days on the vegetable seedling plant that second batch is cultivated, the effective vegetable seedling of numerous aphid is selected as second batch aphid kind strain from the vegetable seedling plant that second batch is cultivated, the aphid selected in the second batch aphid kind strain obtained is inoculated on the 3rd batch of vegetable seedling plant cultivated, every strain vegetable seedling plant inoculates aphid 30；The like carry out connecing aphid, connect aphid once every 20 days, to mid-May；
(2) parasitic aphid is produced
Cocoon honeybee is gathered annual March to field, honeybee is connect to breeding, on vegetable plant strain in step (1) aphid kind room, the cigarette aphid obtained with the cocoon honeybee collected, when connecing honeybee, honeybee aphid is than controlling as 1:50-100, after connecing honeybee, cocoon honeybee forms parasitic aphid for 4-7 days after the cigarette aphid on vegetable plant strain is carried out ovipositing hosts, keep temperature 20-30 DEG C in aphid kind room, humidity 40-70%, produces in aphid kind room and obtains parasitic aphid；
(3) Nicotiana tabacum L. and vegetable fields intercrop
When transplanting tobacco seedlings in vega, the vegetable seedling of Cruciferae in the interplanting of vega periphery, the spacing in the rows of the region tobacco seedlings of interplanting vegetable seedling is 65cm, vegetable seedling interplanting is in the middle of tobacco seedlings with tobacco seedlings, the interplanting quantity of vegetable seedling controls every mu of vega 20-40 strain, water according to conventional water and fertilizer management and execute tobacco seedlings, vegetable seedling is enriched according to conventional dose the nitrogenous fertilizer of 20% 30%；
(4) vegetable seedling in vega is carried out parasitic aphid inoculation preventing and treating aphid evil
Tobacco seedlings and vegetable seedling in vega are interplanted after reaching 20 days, start in cigarette strain in vega that aphid evil occurs, it is inoculated into step (2) aphid kind room produces the parasitic aphid obtained on the vegetable seedling in vega, the parasitic aphid 20-30 head of inoculation on every strain vegetable seedling, carry out the numerous aphid of nature and numerous honeybee preventing and treating aphid evil on vegetable plant strain in vega, in the region of the vegetable radius 1 meter of distance interplanting of whole anti-aphid phase, forbid applying pesticide；
(5) vegetable seedling in vega carries out parasitic aphid mend to take over control and control aphid evil
Vegetable seedling inoculation parasitic aphid in vega is after 5-7 days, adding up to the vegetable seedling of lazy weight 200 to carry out supplement inoculation parasitism aphid 30-50 head aphid on the vegetable plant strain of interplanting in vega and parasitic aphid, in vega, on the vegetable plant strain of interplanting, the most numerous aphid and numerous honeybee prevent and treat aphid evil.
The present invention utilizes the brassicaceous vegetable Seedling of parent aphid to interplant between the cigarette strain and cigarette strain of vega periphery; directly carry out breeding of aphidius gifuensis on the vegetable seedling plant in vega; breed the aphidius gifuensis obtained on vegetable seedling plant and constantly the aphid in cigarette strain in vega is carried out Biological control; the amount of application of chemical pesticide in tobacco planting can be reduced; the ecological environment of protection vega, promotes the safety of Nicotiana tabacum L.；
Use the present invention can reduce conventional method breeding aphidius gifuensis and prevent the required facilities and equipment that eliminates aphis, reduce the cost accounting in plant development, long lasting for ground, the aphidius gifuensis in vega can be helped aphid of strengthening defences, preventing and treating persistent period length, efficiency are high, required numerous honeybee small scale, low cost, breed the aphidius gifuensis resistance obtained, high to the cigarette strain anti-aphid efficiency in vega, it is not necessary to artificially collect and discharge aphidius gifuensis, operation is simple, saves labor.
Detailed description of the invention
Embodiment
1
A kind of in Nicotiana tabacum L. interplant vegetable breed the method that aphidius gifuensis is prevented eliminating aphis, the enforcement step of the method is:
(1) aphid kind is raised
The Cruciferae Corm Brassicae caulorapae dish Seedling of selection aphid hobby, as the cultivation plant of aphid, the annual 1-4 month, the Corm Brassicae caulorapae dish Seedling of the indoor cultivation in batches parent aphid of the aphid kind in booth, cultivates Corm Brassicae caulorapae dish Seedling 20 strain for every batch, a collection of every cultivation in 20 days；nullAfter aphid kind first Corm Brassicae caulorapae dish Seedling indoor is cultivated 20 days，White is gathered to field、Yellow green or green type cigarette aphid，The cigarette aphid collected in field is inoculated in aphid kind room on the plant of first Corm Brassicae caulorapae dish Seedling cultivated，Aphid 30 is connect on every strain Corm Brassicae caulorapae dish Seedling plant，Cigarette aphid growth and breeding after 20 days on the plant of Corm Brassicae caulorapae dish Seedling，The effective Corm Brassicae caulorapae dish Seedling of numerous aphid is selected as first aphid kind strain from the Corm Brassicae caulorapae dish Seedling plant that first is cultivated，The aphid selected in first aphid kind strain obtained is inoculated on the Corm Brassicae caulorapae dish Seedling plant that second batch is cultivated，Aphid 30 is inoculated on every strain Corm Brassicae caulorapae dish Seedling plant，Cigarette aphid growth and breeding after 20 days on the Corm Brassicae caulorapae dish Seedling plant that second batch is cultivated，The effective Corm Brassicae caulorapae dish Seedling of numerous aphid is selected as second batch aphid kind strain from the Corm Brassicae caulorapae dish Seedling plant that second batch is cultivated，The aphid selected in the second batch aphid kind strain obtained is inoculated on the 3rd batch of Corm Brassicae caulorapae dish Seedling plant cultivated，Aphid 30 is inoculated on every strain Corm Brassicae caulorapae dish Seedling plant，The like carry out connecing aphid，Aphid is connect once every 20 days，To mid-May；
(2) parasitic aphid is produced
Aphidius gifuensis is gathered to field annual March, honeybee is connect to breeding, on Corm Brassicae caulorapae plant in step (1) aphid kind room, the cigarette aphid obtained with the aphidius gifuensis collected, connect the honeybee aphid of honeybee than controlling as 1:100, within after cocoon honeybee carries out ovipositing hosts to the cigarette aphid on Corm Brassicae caulorapae plant after connecing honeybee 4 days, form parasitic aphid, keep temperature 20 DEG C in aphid kind room, humidity 40%, produces in aphid kind room and obtains parasitic aphid；
(3) Nicotiana tabacum L. and Herba Astragali Sinici interplanting
When transplanting tobacco seedlings in vega, Herba Astragali Sinici Seedling in the interplanting of vega periphery, the spacing in the rows of the region tobacco seedlings of interplanting Herba Astragali Sinici Seedling is 65cm, the interplanting of Herba Astragali Sinici Seedling is in the middle of tobacco seedlings with tobacco seedlings, the interplanting quantity of Herba Astragali Sinici Seedling controls in every mu of vega 20 strain, water according to conventional water and fertilizer management and execute tobacco seedlings, Herba Astragali Sinici Seedling is enriched according to conventional dose the nitrogenous fertilizer of 20%；
(4) the Herba Astragali Sinici Seedling in vega is carried out parasitic aphid inoculation preventing and treating aphid evil
Tobacco seedlings and Herba Astragali Sinici Seedling in vega are interplanted after reaching 20 days, start in cigarette strain in vega that aphid evil occurs, to step (2) aphid kind room produce on the Herba Astragali Sinici Seedling that the parasitic aphid obtained is inoculated in vega, the parasitic aphid 20 of inoculation on every ZHUHUA dish Seedling, on Herba Astragali Sinici plant in vega, the most numerous aphid and numerous honeybee preventing and treating aphid evil, forbid applying pesticide in the region of the Herba Astragali Sinici Seedling radius 1 meter of distance interplanting of whole anti-aphid phase；
(5) the Herba Astragali Sinici Seedling in vega carries out parasitic aphid mend to take over control and control aphid evil
The parasitic aphid of Herba Astragali Sinici Seedling inoculation in vega is after 5 days, add up to the Herba Astragali Sinici Seedling of lazy weight 200 to carry out supplement inoculation parasitism aphid 30 aphid on the Herba Astragali Sinici plant of interplanting in vega and parasitic aphid, vega carries out the numerous aphid of nature and numerous honeybee to prevent and treat aphid evil on the Herba Astragali Sinici plant of interplanting.
Embodiment
2
A kind of in Nicotiana tabacum L. interplant vegetable breed the method that aphidius gifuensis is prevented eliminating aphis, the enforcement step of the method is:
(1) aphid kind is raised
nullSelect the Cruciferae Corm Brassicae caulorapae dish Seedling cultivation plant as aphid of aphid hobby，The annual 1-4 month，The Corm Brassicae caulorapae dish Seedling of the indoor cultivation in batches parent aphid of the aphid kind in booth，Every batch of cultivation Corm Brassicae caulorapae dish Seedling 100 strain，A collection of every cultivation in 20 days，After aphid kind first Corm Brassicae caulorapae dish Seedling indoor is cultivated 20 days，White is gathered to field、Yellow green or green type cigarette aphid，The cigarette aphid collected in field is inoculated in aphid kind room on the plant of first Corm Brassicae caulorapae dish Seedling cultivated，Aphid 30 is connect on every strain Corm Brassicae caulorapae dish Seedling plant，Cigarette aphid growth and breeding after 20 days on the plant of Corm Brassicae caulorapae dish Seedling，The effective Corm Brassicae caulorapae dish Seedling of numerous aphid is selected as first aphid kind strain from the Corm Brassicae caulorapae dish Seedling plant that first is cultivated，The aphid selected in first aphid kind strain obtained is inoculated on the Corm Brassicae caulorapae dish Seedling plant that second batch is cultivated，Aphid 30 is inoculated on every strain Corm Brassicae caulorapae dish Seedling plant，Cigarette aphid growth and breeding after 20 days on the Corm Brassicae caulorapae dish Seedling plant that second batch is cultivated，The effective Corm Brassicae caulorapae dish Seedling of numerous aphid is selected as second batch aphid kind strain from the Corm Brassicae caulorapae dish Seedling plant that second batch is cultivated，The aphid selected in the second batch aphid kind strain obtained is inoculated on the 3rd batch of Corm Brassicae caulorapae dish Seedling plant cultivated，Aphid 30 is inoculated on every strain Corm Brassicae caulorapae dish Seedling plant，The like carry out connecing aphid，Aphid is connect once every 20 days，To mid-May；
(2) parasitic aphid is produced
Aphidius gifuensis is gathered to field annual March, honeybee is connect to breeding, on Corm Brassicae caulorapae plant in step (1) aphid kind room, the cigarette aphid obtained with the aphidius gifuensis collected, connect the honeybee aphid of honeybee than controlling as 1:50, within after cocoon honeybee carries out ovipositing hosts to the cigarette aphid on Corm Brassicae caulorapae plant after connecing honeybee 7 days, form parasitic aphid, keep temperature 30 DEG C in aphid kind room, humidity 70%, produces in aphid kind room and obtains parasitic aphid；
(3) Nicotiana tabacum L. and Chinese cabbage interplanting
When transplanting tobacco seedlings in vega, the Chinese cabbage Seedling of Cruciferae in the interplanting of vega periphery, the spacing in the rows of the region tobacco seedlings of interplanting Chinese cabbage Seedling is 65cm, the interplanting of Chinese cabbage Seedling is in the middle of tobacco seedlings with tobacco seedlings, the interplanting quantity of Chinese cabbage Seedling controls in every mu of vega 40 strain, water according to conventional water and fertilizer management and execute tobacco seedlings, Chinese cabbage Seedling is enriched according to conventional dose the nitrogenous fertilizer of 30%；
(4) the Chinese cabbage Seedling in vega is carried out parasitic aphid inoculation preventing and treating aphid evil
Tobacco seedlings and Chinese cabbage Seedling in vega are interplanted after reaching 20 days, start in cigarette strain in vega that aphid evil occurs, to step (2) aphid kind room produce on the Chinese cabbage Seedling that the parasitic aphid obtained is inoculated in vega, the parasitic aphid 30 of inoculation on every strain Chinese cabbage Seedling, on Chinese cabbage plant in vega, the most numerous aphid and numerous honeybee preventing and treating aphid evil, forbid applying pesticide in the region of the Chinese cabbage plant radius 1 meter of distance interplanting of whole anti-aphid phase；
(5) the Chinese cabbage Seedling in vega carries out parasitic aphid mend to take over control and control aphid evil
The parasitic aphid of Chinese cabbage Seedling inoculation in vega is after 7 days, add up to the Chinese cabbage Seedling of lazy weight 200 to carry out supplement inoculation parasitism aphid 50 aphid on the Chinese cabbage plant of interplanting in vega and parasitic aphid, vega carries out the numerous aphid of nature and numerous honeybee to prevent and treat aphid evil on the Chinese cabbage plant of interplanting.
Claims (1)
1. in Nicotiana tabacum L., interplant vegetable breed the method that aphidius gifuensis is prevented eliminating aphis, it is characterised in that: the method includes following enforcement step:
(1) aphid kind is raised
The brassicaceous vegetable Seedling of selection aphid hobby, as the cultivation plant of aphid, the annual 1-4 month, the brassicaceous vegetable Seedling of the indoor cultivation in batches parent aphid of the aphid kind in booth, every batch of culturing vegetable Seedling 20-100 strain, was cultivated a collection of every 20 days；After aphid kind first vegetable seedling indoor is cultivated 20 days, gathering white, yellow green or green type cigarette aphid to field, the cigarette aphid collected in field is inoculated in aphid kind room on the plant of first vegetable seedling cultivated, every strain vegetable seedling plant connects aphid 30；Cigarette aphid growth and breeding after 20 days on the plant of vegetable seedling, the effective vegetable seedling of numerous aphid is selected as first aphid kind strain from the vegetable seedling plant that first is cultivated, the aphid selected in first aphid kind strain obtained is inoculated on the vegetable seedling plant that second batch is cultivated, every strain vegetable seedling plant inoculates aphid 30；Cigarette aphid growth and breeding after 20 days on the vegetable seedling plant that second batch is cultivated, the effective vegetable seedling of numerous aphid is selected as second batch aphid kind strain from the vegetable seedling plant that second batch is cultivated, the aphid selected in the second batch aphid kind strain obtained is inoculated on the 3rd batch of vegetable seedling plant cultivated, every strain vegetable seedling plant inoculates aphid 30；The like carry out connecing aphid, connect aphid once every 20 days, to mid-May；
(2) parasitic aphid is produced
Cocoon honeybee is gathered annual March to field, honeybee is connect to breeding, on vegetable plant strain in step (1) aphid kind room, the cigarette aphid obtained with the cocoon honeybee collected, connect the honeybee aphid of honeybee than controlling as 1:50-100, after connecing honeybee, cocoon honeybee forms parasitic aphid for 4-7 days after the cigarette aphid on vegetable plant strain is carried out ovipositing hosts, keep temperature 20-30 DEG C in aphid kind room, humidity 40-70%, produces in aphid kind room and obtains parasitic aphid；
(3) Nicotiana tabacum L. and vegetable fields intercrop
When transplanting tobacco seedlings in vega, the vegetable seedling of Cruciferae in the interplanting of vega periphery, the spacing in the rows of the region tobacco seedlings of interplanting vegetable seedling is 65cm, vegetable seedling interplanting is in the middle of tobacco seedlings with tobacco seedlings, the interplanting quantity of vegetable seedling controls every mu of vega 20-40 strain, water according to conventional water and fertilizer management and execute tobacco seedlings, vegetable seedling is enriched according to conventional dose the nitrogenous fertilizer of 20% 30%；
(4) vegetable seedling in vega is carried out parasitic aphid inoculation preventing and treating aphid evil
Tobacco seedlings and vegetable seedling in vega are interplanted after reaching 20 days, start in cigarette strain in vega that aphid evil occurs, it is inoculated into step (2) aphid kind room produces the parasitic aphid obtained on the vegetable seedling in vega, the parasitic aphid 20-30 head of inoculation on every strain vegetable seedling, carry out the numerous aphid of nature and numerous honeybee preventing and treating aphid evil on vegetable plant strain in vega, in the region of the vegetable radius 1 meter of distance interplanting of whole anti-aphid phase, forbid applying pesticide；
(5) vegetable seedling in vega carries out parasitic aphid mend to take over control and control aphid evil
Vegetable seedling inoculation parasitic aphid in vega is after 5-7 days, adding up to the vegetable seedling of lazy weight 200 to carry out supplement inoculation parasitism aphid 30-50 head aphid on the vegetable plant strain of interplanting in vega and parasitic aphid, in vega, on the vegetable plant strain of interplanting, the most numerous aphid and numerous honeybee prevent and treat aphid evil.
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| CN112243957A (en) * | 2020-10-26 | 2021-01-22 | 云南农业大学 | Method for colonization of aphidius gifuensis ashmead by planting endive |
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| CN105145490B (en) * | 2015-08-21 | 2018-10-12 | 山东临沂烟草有限公司 | A kind of method and its application of vegetable seedling breeding aphidius gifuensis |
| CN105104311A (en) * | 2015-09-25 | 2015-12-02 | 黔南州烟草公司瓮安县分公司 | Method for breeding aphidius gifuensis ashmeads through alternanthera philoxeroides |
| CN109892132A (en) * | 2019-03-18 | 2019-06-18 | 中国农业科学院烟草研究所 | A kind of artificial natural enemy protection island |
| CN110122249A (en) * | 2019-05-14 | 2019-08-16 | 潍坊市烟草专卖局 | A kind of insect pest control method of tobacco field |
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| CN101455190B (en) * | 2008-12-30 | 2011-11-02 | 云南省烟草公司玉溪市公司 | Aphidius gifuensis Ashmaed scale propagation technique |
| EP2471353A1 (en) * | 2010-12-30 | 2012-07-04 | Philip Morris Products S.A. | A method of growing tobacco plants |
| CN102812933B (en) * | 2012-09-11 | 2014-02-05 | 云南省烟草公司玉溪市公司 | Overwintering preserving breeding method for aphidius gifuensis |
| CN102870586A (en) * | 2012-10-26 | 2013-01-16 | 玉溪市农业科学院 | High-yield planting method suitable for flue-cured interplanting of rape in mountainous region |
| CN103168751B (en) * | 2013-04-07 | 2015-05-20 | 中国农业科学院植物保护研究所 | Expanding propagation method for aphidius gifuensis ashmeads |
| CN103478083B (en) * | 2013-09-29 | 2015-03-11 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN103749139B (en) * | 2014-01-22 | 2015-10-21 | 云南省烟草公司玉溪市公司 | The biological control method of a kind of crop rotation vega cigarette aphid |
| CN103999665B (en) * | 2014-05-09 | 2016-08-24 | 云南省烟草公司保山市公司 | A kind of method with certain herbaceous plants with big flowers dish cultivation aphidius gifuensis |
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| CN112243957A (en) * | 2020-10-26 | 2021-01-22 | 云南农业大学 | Method for colonization of aphidius gifuensis ashmead by planting endive |
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