CN113728981A - Method for researching selective influence of volatile matter on oviposition behavior of spodoptera frugiperda - Google Patents
Method for researching selective influence of volatile matter on oviposition behavior of spodoptera frugiperda Download PDFInfo
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- CN113728981A CN113728981A CN202111118775.5A CN202111118775A CN113728981A CN 113728981 A CN113728981 A CN 113728981A CN 202111118775 A CN202111118775 A CN 202111118775A CN 113728981 A CN113728981 A CN 113728981A
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- spodoptera frugiperda
- inner cylinder
- black box
- nutrient solution
- gauze
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- 241000256251 Spodoptera frugiperda Species 0.000 title claims abstract description 56
- 230000006399 behavior Effects 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000017448 oviposition Effects 0.000 title claims abstract description 16
- 235000013601 eggs Nutrition 0.000 claims abstract description 42
- 235000015097 nutrients Nutrition 0.000 claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 240000008042 Zea mays Species 0.000 claims description 17
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 17
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 17
- 235000005822 corn Nutrition 0.000 claims description 17
- 239000003039 volatile agent Substances 0.000 claims description 17
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 14
- 241000209094 Oryza Species 0.000 claims description 13
- 235000007164 Oryza sativa Nutrition 0.000 claims description 10
- 235000009566 rice Nutrition 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 235000013339 cereals Nutrition 0.000 claims description 4
- 241001057636 Dracaena deremensis Species 0.000 claims description 3
- 240000001624 Espostoa lanata Species 0.000 claims description 3
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 3
- 235000012907 honey Nutrition 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000012353 t test Methods 0.000 claims description 3
- 238000011160 research Methods 0.000 abstract description 9
- 238000012216 screening Methods 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 3
- 239000008187 granular material Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 241000256248 Spodoptera Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000001846 repelling effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000001617 migratory effect Effects 0.000 description 2
- 244000077995 Coix lacryma jobi Species 0.000 description 1
- 240000008620 Fagopyrum esculentum Species 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a method for researching the selective influence of volatile matters on the oviposition behavior of spodoptera frugiperda, which needs to manufacture a culture device, wherein the culture device comprises a black box and an inner cylinder; the black box is of an openable and closable structure; the inner cylinder is arranged in the black box and is of an internal hollow structure, nutrient solution is placed in the inner cylinder, and removable and replaceable gauzes are arranged on two opposite ports of the inner cylinder; can place Spodoptera frugiperda inside it at this moment and lay eggs to through observing the circumstances of laying eggs at Spodoptera frugiperda on different gauze, then can carry out the analysis to Spodoptera frugiperda's the action of laying eggs, thereby solved the problem that prior art lacks the selective action research and the action regulator screening method that are used for Spodoptera frugiperda to lay eggs.
Description
Technical Field
The invention relates to the technical field of Spodoptera frugiperda research, in particular to a method for researching selective influence of volatile matters on egg laying behavior of Spodoptera frugiperda.
Background
Spodoptera frugiperda is a global early-warning migratory pest newly invading China, and has the characteristics of multiple host types, strong reproductive capacity, large larva food intake, fast migratory flight diffusion, high prevention and control difficulty and the like. Due to the characteristic of boring damage of the worm, a good prevention effect is difficult to achieve by using a chemical prevention and control means.
In recent years, a new technology for controlling pests by using insect behavior regulating substances is widely applied, and the monitoring, trapping, disorienting, repelling and the like can be performed on the pests. Compared with the traditional chemical pesticide, the technology is an environment-friendly plant protection strategy.
Earlier researches find that Spodoptera frugiperda also shows obvious difference in egg-laying plant selection, preferentially selects to lay eggs on gramineous plants such as corn and coix seeds, and dislikes to lay eggs on tobacco, rice and buckwheat. Spodoptera frugiperda is more prone to lay eggs on the back of corn leaves, and less on the front of leaves. Therefore, volatile matters capable of repelling spawning of spodoptera frugiperda are screened through indoor behavior experiments, and are optimally combined into a repelling formula, so that green prevention and control of spodoptera frugiperda are facilitated. However, at present, there is no technical method for egg laying selection behavior test of spodoptera frugiperda adults, which is used for egg laying selection behavior research of spodoptera frugiperda and screening of behavior regulators.
Disclosure of Invention
The invention aims to provide a method for researching the selective influence of volatile matters on the oviposition behavior of spodoptera frugiperda, and aims to solve the problem that the prior art is lack of methods for researching the oviposition selective behavior of spodoptera frugiperda and screening behavior regulators.
In order to solve the technical problems, the invention provides a method for researching the selective influence of volatile matters on the oviposition behavior of spodoptera frugiperda, which comprises the following steps,
step S1, manufacturing a culture device, wherein the culture device comprises a black box and an inner cylinder; the black box is of an openable and closable structure; the inner cylinder is arranged in the black box and is of an internal hollow structure, nutrient solution is placed in the inner cylinder, and removable and replaceable gauzes are arranged on two opposite ports of the inner cylinder;
step S2, placing nutrient solution in the inner barrel, placing a plurality of pairs of spodoptera frugiperda male and female adults in the inner barrel, sealing the port of the inner barrel by using the gauze, and adjusting the temperature and humidity of an experimental environment to preset values;
step S3, respectively spraying different volatile matters on the two gauze screens, and closing the black box;
step S4, spraying volatile matters on the gauze again every 8-12 hours;
step S5, recording the number of egg masses on the gauze every 24 hours as X1、X2、X3···XnAnd number of eggs Y1、Y2、Y3···YnStopping collecting data when the number of spawning grains is less than 10 grains in every 24 hours;
step S5, counting the total amount X ═ Sigma X of egg masses of Spodoptera frugiperda in the whole egg laying periodnAnd the total amount of eggs Y ═ Σ YnAnd the different treatment groups are respectively tested for the difference significance by using a t test.
In one embodiment, in step S2, a cotton ball soaked with the nutrient solution is placed in the inner cylinder.
In one embodiment, the nutrient solution is honey water or sugar water.
In one embodiment, in step S2, the temperature of the test environment is set to 27 + -1 deg.C and the humidity of the test environment is set to 70 + -5%.
In one embodiment, in step S2, 4-5 pairs of newly emerged male and female spodoptera frugiperda adults are placed in the inner barrel.
In one embodiment, in step S3, one of the screens is sprayed with corn volatiles or rice volatiles and the other screen is sprayed with n-hexane.
In one embodiment, the corn volatiles are extracted from healthy corn plants in the small flare stage; the rice volatile matter is obtained by extracting healthy rice plants in the jointing and booting stage.
In one embodiment, the nutrient solution is randomly placed, and the placement position is changed every 8-12 hours.
The invention has the following beneficial effects:
after the purpose-built culture apparatus of preparation, can place Spodoptera frugiperda in its inside and lay eggs, because all be equipped with the gauze that can dismantle the change on the relative both ends mouth of inner tube, so through observing the circumstances of laying eggs of Spodoptera frugiperda on different gauzes, then can carry out the analysis to the behavior of laying eggs of Spodoptera frugiperda to the problem that prior art lacks the selective behavior research and the behavior regulator screening method that are used for Spodoptera frugiperda to lay eggs has been solved consciently.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of a culture apparatus provided in an embodiment of the present invention;
FIG. 2 is a statistical table of the number of eggs obtained when corn volatiles were sprayed;
FIG. 3 is a statistical table of the number of eggs obtained when corn volatiles were sprayed;
FIG. 4 is a statistical table of the number of eggs obtained when corn volatiles were sprayed;
FIG. 5 is a statistical table of the number of eggs obtained when spraying with corn volatiles.
The reference numbers are as follows:
100. a culture device;
10. a black box;
20. an inner barrel; 21. a frustum-shaped pipe section;
30. a gauze.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
The present invention provides a method for studying the selective influence of volatiles on oviposition behavior of spodoptera frugiperda, embodiments of which are shown in figures 1 to 5, comprising the steps of,
step S1, manufacturing a culture device 100, wherein the culture device 100 comprises a black box 10 and an inner cylinder 20; the black box 10 is an openable structure; the inner cylinder 20 is arranged in the black box 10, the inner cylinder 20 is of an internal hollow structure, nutrient solution is placed in the inner cylinder 20, and the two opposite ports of the inner cylinder 20 are respectively provided with a detachable and replaceable gauze 30;
step S2, placing nutrient solution in the inner barrel 20, placing a plurality of pairs of spodoptera frugiperda male and female adults in the inner barrel 20, sealing the port of the inner barrel 20 by using a gauze 30, and setting the temperature and humidity of the experimental environment to preset values;
step S3, respectively spraying different volatile matters on the two gauze elements 30, and closing the black box 10;
step S4, spraying volatile matter on the gauze 30 again every 8-12 hours;
step S5, recording the number of egg masses on the gauze 30 as X1, X2, X3 & cndot and the number of egg granules as Y1, Y2 and Y3 & cndot every 24 hours, and stopping collecting data when the number of egg granules is less than 10 granules in every 24 hours;
and step S5, counting the total amount X of the egg masses and the total amount Y of the egg granules of the spodoptera frugiperda in the whole egg laying period, wherein the total amount X of the egg masses and the total amount Y of the egg granules are sigma Yn, and respectively testing the difference significance of different treatment groups by using t test.
So after making purpose-built culture apparatus 100, can place Spodoptera frugiperda inside it and lay eggs to through observing the circumstances of laying eggs at Spodoptera frugiperda on different gauze 30, then can carry out the analysis to Spodoptera frugiperda's the action of laying eggs, thereby solved the problem that prior art lacks the selective behavior research and the action regulator screening method that are used for Spodoptera frugiperda to lay eggs.
In step 1, the opening structure of the black box 10 has various embodiments, for example, a side wall of the black box 10 may be connected by a hinge to control the opening and closing of the black box 10 in a folded manner, or a side wall of the black box 10 may slide in a sliding slot to control the opening and closing of the black box 10; the gauze 30 can be sleeved outside the inner barrel 20 by using rubber bands, or magic tapes are arranged on the gauze 30 and the inner barrel 20, so that the detachable installation of the gauze 30 can be realized.
In addition, the culture device 100 can be improved to obtain better use effect.
As shown in fig. 1, the inner wall of the inner cylinder 20 is preferably smooth in this embodiment.
After adopting this mode of setting up, Spodoptera frugiperda will be more difficult to lay eggs on the inner wall of inner tube 20 to better restriction Spodoptera frugiperda to lay eggs on gauze 30, provide better environment for the research to Spodoptera frugiperda.
As shown in fig. 1, the inner cylinder 20 is preferably a black non-light-transmitting structure in this embodiment.
After adopting this mode of setting up, will realize the secondary shading by black case 10 and inner tube 20 to the simulation is more real night environment, thereby better promotion Spodoptera frugiperda to lay eggs.
As shown in fig. 1, the inner cylinder 20 of this embodiment is preferably provided with two frustum-shaped pipe sections 21, the small-caliber ends of the two frustum-shaped pipe sections 21 are connected and communicated with each other, and the large-caliber ends of the two frustum-shaped pipe sections 21 are covered with the gauze 30.
After adopting this mode of arrangement, inner tube 20 will be the shape of middle narrow both ends mouth, so according to the meadow spodoptera likes the characteristic of flying toward open place, the meadow spodoptera will more tend to fly to the position at gauze 30 place to further promoted the meadow spodoptera and laid eggs on gauze 30.
Aiming at the step S2, the nutrient solution can be placed in various ways, for example, the nutrient solution can be placed directly on a vessel, but in this embodiment, the cotton ball soaked with the nutrient solution is preferably placed in the inner barrel 20, and this way has the advantages that the storage of the nutrient solution can be realized, the nutrient solution can attract spodoptera frugiperda to suck, and the situation that the spodoptera frugiperda cannot move after being adhered is not easy to occur; in order to meet the eating preference and the nutritional requirement of the spodoptera frugiperda, the nutrient solution is preferably honey water or sugar water.
In addition, in order to ensure the accuracy of the experimental result, the nutrient solution is preferably placed randomly in the embodiment, and the placing position is changed every 8-12 hours, so that the tendency influence of the experiment caused by the fixed placement of the nutrient solution is avoided.
Furthermore, in order to provide a more suitable spawning environment for spodoptera frugiperda, the temperature of the experimental environment is preferably set to be 27 +/-1 ℃ and the humidity of the experimental environment is preferably set to be 70 +/-5% in the embodiment, so that the requirements of the spodoptera frugiperda on the temperature and the humidity are met; in addition, in order to ensure the optimal spawning effect, the embodiment preferably places 4 to 5 pairs of newly emerged male and female spodoptera frugiperda adults into the inner barrel 20, so as to ensure the consistency of the experimental objects and obtain the optimal spawning comparison effect.
To step S3, through the different volatile matters of spraying on two gauze 30, then the accessible number of laying eggs judges which kind of volatile matter is stronger to spodoptera frugiperda 'S attraction line, closes black case 10 back in addition, also can build the night environment to this improves spodoptera frugiperda' S efficiency of laying eggs.
In this embodiment, it is preferable to spray corn volatiles or rice volatiles on one of the screens 30 and n-hexane on the other screen 30, so as to form a comparison, for example, when the corn volatiles and n-hexane are sprayed on the two screens 30, respectively, it can be known which of the corn volatiles and n-hexane is more attractive to spodoptera frugiperda, and similarly, the rice volatiles are also the same.
In addition, in order to extract volatile matters with better quality, the corn volatile matters are obtained by selecting healthy corn plants in a small trumpet stage and the rice volatile matters are obtained by selecting healthy rice plants in a jointing and booting stage, so that the volatile matters can provide accurate reference for behavior research of spodoptera frugiperda.
For example, two comparisons were made in this example, one for corn volatiles versus n-hexane, and the results are shown in FIGS. 2 and 3, indicating that Spodoptera frugiperda prefers to lay eggs on a screen 30 coated with corn volatiles; the other group is the comparison of the rice volatile matter and n-hexane, and the experimental results are shown in fig. 4 and 5, thereby indicating that spodoptera frugiperda prefers to lay eggs on the n-hexane-sprayed gauze 30; according to the two groups of comparison results, the corn volatile matter has the greatest attraction to Spodoptera frugiperda, and the rice volatile matter has the least attraction to Spodoptera frugiperda, so that the requirement on the research on the oviposition behavior of Spodoptera frugiperda can be completely met through the experiment.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.
Claims (8)
1. A method for researching the selective influence of volatile matters on the oviposition behavior of Spodoptera frugiperda is characterized by comprising the following steps,
step S1, manufacturing a culture device, wherein the culture device comprises a black box and an inner cylinder; the black box is of an openable and closable structure; the inner cylinder is arranged in the black box and is of an internal hollow structure, nutrient solution is placed in the inner cylinder, and removable and replaceable gauzes are arranged on two opposite ports of the inner cylinder;
step S2, placing nutrient solution in the inner barrel, placing a plurality of pairs of spodoptera frugiperda male and female adults in the inner barrel, sealing the port of the inner barrel by using the gauze, and adjusting the temperature and humidity of an experimental environment to preset values;
step S3, respectively spraying different volatile matters on the two gauze screens, and closing the black box;
step S4, spraying volatile matters on the gauze again every 8-12 hours;
step S5, recording the number of egg masses on the gauze every 24 hours as X1、X2、X3···XnAnd number of eggs Y1、Y2、Y3···YnStopping collecting data when the number of spawning grains is less than 10 grains in every 24 hours;
step S5, counting the total amount X ═ Sigma X of egg masses of Spodoptera frugiperda in the whole egg laying periodnAnd the total amount of eggs Y ═ Σ YnAnd the different treatment groups are respectively tested for the difference significance by using a t test.
2. The method of claim 1, wherein in step S2, the cotton balls soaked with the nutrient solution are placed in the inner cylinder.
3. The method of claim 2, wherein the nutrient solution is honey water or sugar water.
4. The method of claim 1, wherein in step S2, the temperature of the experimental environment is set to 27 ± 1 ℃ and the humidity of the experimental environment is set to 70 ± 5%.
5. The method according to claim 1, wherein in step S2, 4 to 5 pairs of newly emerged male and female Spodoptera frugiperda adults are placed in the inner tub.
6. The method of claim 1, wherein in step S3, one of the screens is sprayed with corn volatiles or rice volatiles and the other screen is sprayed with n-hexane.
7. The method of claim 6,
the corn volatile matter is obtained by extracting a healthy corn plant in a small trumpet-shaped period;
the rice volatile matter is obtained by extracting healthy rice plants in the jointing and booting stage.
8. The method according to claim 1, wherein the nutrient solution is randomly placed and the placement position is changed every 8-12 hours.
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