CN110432231A - The method of single head raising South America tomato leaf miner - Google Patents
The method of single head raising South America tomato leaf miner Download PDFInfo
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- CN110432231A CN110432231A CN201910834842.XA CN201910834842A CN110432231A CN 110432231 A CN110432231 A CN 110432231A CN 201910834842 A CN201910834842 A CN 201910834842A CN 110432231 A CN110432231 A CN 110432231A
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 241000594033 Liriomyza bryoniae Species 0.000 title claims abstract 13
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract description 18
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 15
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 15
- 210000004681 ovum Anatomy 0.000 claims abstract description 15
- 240000003768 Solanum lycopersicum Species 0.000 claims description 17
- 229920000742 Cotton Polymers 0.000 claims description 14
- 239000003755 preservative agent Substances 0.000 claims description 7
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 244000061458 Solanum melongena Species 0.000 claims description 2
- 235000002597 Solanum melongena Nutrition 0.000 claims description 2
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- 241000238631 Hexapoda Species 0.000 abstract description 22
- 241000196324 Embryophyta Species 0.000 abstract description 18
- 241000382353 Pupa Species 0.000 abstract description 15
- 229920003023 plastic Polymers 0.000 abstract description 7
- 230000003031 feeding effect Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 2
- 241000227653 Lycopersicon Species 0.000 abstract 1
- 241001389006 Tuta absoluta Species 0.000 description 27
- 238000011161 development Methods 0.000 description 8
- 230000018109 developmental process Effects 0.000 description 8
- 230000008144 egg development Effects 0.000 description 7
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 241000366676 Justicia pectoralis Species 0.000 description 4
- 230000012447 hatching Effects 0.000 description 4
- 238000005065 mining Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 239000002390 adhesive tape Substances 0.000 description 3
- 230000032669 eclosion Effects 0.000 description 3
- 210000001503 joint Anatomy 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000004382 potting Methods 0.000 description 3
- 241000255777 Lepidoptera Species 0.000 description 2
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- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000019617 pupation Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
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- 241001517923 Douglasiidae Species 0.000 description 1
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- 235000003953 Solanum lycopersicum var cerasiforme Nutrition 0.000 description 1
- 240000003040 Solanum lycopersicum var. cerasiforme Species 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to cultured insect fields, and in particular to a kind of method of single head raising South America tomato leaf miner.The method of single head raising South America tomato leaf miner of the invention, is included in transparent raising box, using fresh-keeping tomato leaf as host plant, the step of South America tomato leaf miner is raised by ovum to pupa.The present invention is not only good to the feeding effect of ovum but also also relatively good to the feeding effect of larva and pupa when single head raises South America tomato leaf miner；Box is raised using cylindrical transparent plastics, raising box material is fully transparent, can directly observe, convenient and efficient；The fresh keeping time of host's blade is long；Therefore, method of the invention is practical, can meet the needs of single head cultured insect and its subsequent correlative study.
Description
Technical field
The invention belongs to cultured insect fields, and in particular to the method for single head raising South America tomato leaf miner.
Background technique
South America tomato leaf miner Tuta absoluta (Meyrick), alias tomato leaf miner, tomato latent gelechiid, tomato wheat
Moth, English first choice adopted name the tomato leafminer, international common name the South American tomato
Moth belongs to Lepidoptera (Lepidoptera) Gelechidae (Gelechiidae), originates in the Peru in South America western part, be a kind of generation
Criticality quarantine invades pest, main harm tomato (including processing tomato, fresh food tomato, cherry and tomato/cherry tomato etc.), eggplant
Son, potato, mushroom ma, panax ginseng fruit, tobacco etc., and spread rapidly, underproduction rate is high up to 50~80% when seriously occurring, or even ruins
Kind replay.Although current South America tomato leaf miner only occurs in China's regional area, protecting field and open country tomato are caused
It seriously endangers, in order to study the control method of South America tomato leaf miner, effectively solves tomato crop and asked by what is seriously endangered
Topic, it is necessary to solve the problems, such as the raising of South America tomato leaf miner.
The problems such as high there are insect unusual death ratio due to the blade raising insect to be detached from plant, the prior art is raised
Supporting South America tomato leaf miner mainly uses potting host to shroud raising, mainly includes following several method:
1) micro-blade dependent insect cage single head method for breeding: it is mainly used for raising the small insects such as aphid, aleyrodid；Host plant
The plant directly planted for potting or field.Culture vessel is by spun yarn, lucite tube and buffering sponge, seccotine, metal
The composition such as folder.When raising, directly culture vessel is clipped on the blade for being connected to insect with metal clip.2) micro-blade spun yarn bag
Single head method for breeding: host plant is potting or the plant that field is directly planted.Culture vessel is spun yarn bag, by spun yarn, tightening
The composition such as rope, is sutured, length, width and thickness are respectively 12-15cm, 8-10cm and 2.5-3cm with sewing machine.When raising, directly
It connects and ties up to spun yarn bag cover on blade.3) transparent happy button box single head method for breeding: host plant is hydroponic plant.Culture vessel is by two
The happy button box (diameter and height be respectively 10cm and 15+15cm) of a circular non-opaque, plastic foam plate, tap water, sealant tape,
Cover the composition such as spun yarn air hole.
However, the first and second method have the following deficiencies: 1, due to the weight of culture vessel depend on it is single
Blade frequently results in blade broken, falls off in advance, and insect is not and the complete blade of development has just dried up；2, since culture vessel is solid
It cannot arbitrarily be rotated after fixed, can only observe a certain angle of blade, be unfavorable for observing, it is particularly disadvantageous in ovum and first ovum larva
Observation；When through containing the observation of the culture vessel of spun yarn or spun yarn bag, the visual field is fuzzy, and especially to leaf mining class pest, (such as leaf miner is dived
Leaf fly etc.) observing effect it is bad；3, because space is smaller, blade area is limited, and larva can also be left by potential food mine to be shrouded
Region, so that single head is raised and is observed can not continue, and experiment is forced to terminate；4, potted plant occupied space is bigger, can only
It is carried out in field, greenhouse or Minitype manual climatic chamber, the environmental condition in field and greenhouse is more difficult to control, and Minitype manual weather
Room, though can control temperature and humidity, raising scale is extremely limited, for example, volume be in the Minitype manual climatic chamber of 450L most
4 plants of potted plants can only mostly be put, one plant of plant based on 10 true leaves, can only simultaneously about 40 insects of breeding observing.
The defect of the third method is that culture vessel is unable to side, (side, inversion will lead to water planting water and incline for inversion observation
Spill), cause worm age, worm state to distinguish unclear, observation result inaccuracy, especially to leaf mining class insect and ovum (about 0.3mm) and
The observation result inaccuracy of newly hatched larvae (body is about 0.5mm)；The raising scale of this method is also difficult to scale up, for example, an appearance
Product is that the Minitype manual climatic chamber of 450L can only at most observe 40-50 insects of raising simultaneously.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of single head raising South America tomato leaf miner.
The method of the single head raising South America tomato leaf miner of specific embodiment according to the present invention comprising in transparent raising
The step of being host with fresh-keeping tomato leaf in box, South America tomato leaf miner raised by ovum to adult.
The method of the single head raising South America tomato leaf miner of specific embodiment according to the present invention, the fresh-keeping tomato leaf
Piece is made of method comprising the following steps: cutting from petiole, the petiolate tomato leaf of the company of obtaining, and to be saturated water suction
Absorbent cotton swathes the petiole.
The method of the single head raising South America tomato leaf miner of specific embodiment according to the present invention, is swathed described with absorbent cotton
After petiole, then with preservative film swathe the absorbent cotton；Absorbent cotton is folded up 180 ° from end 1/3~1/2, again with guarantor
Fresh film is at swathing the absorbent cotton；Mediate the upper end of the preservative film and the joint of the petiole.
The method of the single head raising South America tomato leaf miner of specific embodiment, the length of the petiole are according to the present invention
45-50mm；The length of the absorbent cotton is 80-100mm, width is 30-40mm, with a thickness of 2.5-3.5mm.
The method of the single head raising South America tomato leaf miner of specific embodiment according to the present invention, the transparent raising box
Shape be cylinder, and it is described it is transparent raising box include mounted on top upper raising box and lower raising box, the upper raising box and
The diameter of the lower raising box is highly equal, and the upper raising box is connected to up and down with the lower raising box.
The method of the single head raising South America tomato leaf miner of specific embodiment according to the present invention, the upper raising box, institute
It states and is equipped with opening on the bottom surface of lower raising box, the upper raising box and the lower raising box pass through the open communication.
The method of the single head raising South America tomato leaf miner of specific embodiment according to the present invention, along the upper raising box and
At the open butt joint of the lower raising box, fixed with multistage transparent plastic tape-stripping, the spacing between multiple adhesive tapes is equal.
The single head of the specific embodiment according to the present invention of specific embodiment raises South America tomato leaf mining according to the present invention
The diameter of the method for moth, the raising box is 80-90mm, is highly 10-15mm.
Beneficial effects of the present invention:
(1) present invention is when single head raises South America tomato leaf miner, not only to the feeding effect of ovum, larva and pupa compared with
It is good, wherein egg hatching is that the ratio (hatching rate) of larva is 100%, is by the ratio (percentage of pupation) that larvae development is pupa
65.6%, it is 63.6% by the survival rate that egg development is adult that the ratio (eclosion rate) that adult is turned by pupa, which is 96.9%, because
This, method feeding effect of the invention is good；
(2) present invention raises box using cylindrical transparent plastics, and raising box material is fully transparent, without that will raise when observation
Box is opened, and can directly be observed, convenient and efficient, is particularly suitable for observation leaf mining class pest, while being also avoided that larva escapes；
(3) raising box of the invention is simple for production, low in cost, saves space, and raising box production can be completed within two minutes,
Each raising box cost less than 1 yuan, both can level put or pile up up and down, be the artificial lighting culture of 450L with a volume
For case, 300 raising boxes can be at least piled up simultaneously, and therefore, the present invention can breeding observing 300 or more elder brothers simultaneously
Worm realizes extensive raising；
(4) fresh keeping time of host's blade is long, and each fresh-cut host's blade is full freshness-retained 2 weeks or more under the conditions of 25 DEG C
The entire larval phase growth requirement of foot, and host replaces convenient, branches and leaves trunk snap easy to break when avoiding using potted plant as host plant
It is withered, the problem of raising can not be continued；Meanwhile the blade to be detached from plant further improves culture vessel and is unable to side as host plant
It sets, be inverted, observe inconvenient problem, be varied as desired the viewing angle of raising box, so as to quickly determine insect hair
State is educated, has been saved observing time, accuracy is improved；
(5) it in order to ensure that raising box inner air flow and raising box are firm, changes into adhesive tape, equidistant interval is viscous
Patch, is conducive to air circulation and blade is fresh-keeping.
Therefore, method of the invention is practical, can meet the needs of single head cultured insect and its subsequent correlative study.
Detailed description of the invention
Fig. 1 shows the structural schematic diagram of transparent raising box used in the embodiment of the present invention, wherein box is raised on 1-,
Box is raised under 2-；
Fig. 2 shows fresh-keeping fresh-cut host's blade；
Fig. 3 shows each worm of the raising ovum of South America tomato leaf miner of specific embodiment according to the present invention, larva, pupa
The entire development time of (ovum-adult) before state development duration and adult；
Fig. 4 shows each worm of the raising ovum of South America tomato leaf miner of specific embodiment according to the present invention, larva, pupa
State survival rate and by egg development be adult survival rate；
Fig. 5 shows the female and male insect ratio of the raising South America tomato leaf miner of specific embodiment according to the present invention
Rate.
Specific embodiment
Embodiment 1
As shown in Figure 1, the shape that the present invention is used to raise transparent raising box used in the tomato leaf miner of South America is cylinder,
And the transparent raising box includes the upper raising box 1 and lower raising box 2 of mounted on top, the upper raising box 1 and the lower raising
The diameter of box 2 is highly equal, wherein diameter and height respectively 85mm and 13mm.The upper raising box 1, the lower raising
It is equipped with opening on the bottom surface of box 2, the opening and open butt joint of two boxes, constitute diameter and be 85mm, be highly 26mm's when use
Cylinder raising box, it is spare.
Acquisition cleaning fresh-cut tomato leaf, contains 1~3 leaflet thereon, the long 45~50mm of petiole, with sharp cutter by petiole
An oblique section is cut into, in favor of moisture absorption.It is swathed with the absorbent cotton of the saturation water suction of wide 35mm, thickness 3mm and long 90mm
Petiole；2 circles are swathed with the preservative film that width and length are respectively 70mm and 140mm again, then in the end of absorbent cotton 1/3~1/
180 ° are folded up at 2, then row swathes 2 circles, and mediates preservative film upper end and petiole joint, so that blade moisturizing is fresh-keeping, such as
Shown in Fig. 2.
When raising South America tomato leaf miner, fresh-keeping fresh-cut host's blade is put into cylindrical transparent plastics raising box, is connect
Enter insect ovum or larva, then at the open butt joint of two shallow boxes of transparent plastic, with width be 12mm and length is the 3 of 40mm
Section transparent plastic adhesive tape carries out stickup fixation with the spacing distance of 45~50mm；The position of non-Continuous pressing device for stereo-pattern, to air stream
It is logical.
The raising situation of the investigation South America tomato leaf miner of embodiment 2
Using South America tomato leaf miner as experimental subjects, to eat tomato raw as host plant, will have ovum (ovum phase about 12h)
It is put into after tomato leaf Preservation Treatment in cylindrical transparent plastics raising box, observes and records the state and color of a deutovum daily
Variation；A larvae development state is observed and recorded to light daily after larvae hatch, until larva turns to pupa.It observes and records daily
The state of 1 pupa, until adult eclosion.Single head/grain raising, every 1 batch, 8~10/repeat 4 batches.
The development duration of every ovum, every larva or pupa is calculated, and goes through the phase by egg development for adult.
Experimental result is as shown in figure 3, the ovum phase is 6.81 ± 0.21 days, and larval phase is 15.86 ± 0.20 days, pupa time 9.23
± 0.53 day；Development duration before adult, also that is, being 31.90 ± 0.93 days by the phase of entirely going through that egg development is adult.
It counts every batch of and turns to the quantity of pupa for the quantity of larva, by larva by egg hatching, and adult is turned by pupa
Quantity, and calculate accordingly each worm state survival rate and by egg development be adult ratio.
Experimental result as shown in figure 4, hatching rate, i.e., is 100.0 ± 0.0% by the ratio that egg development is larva；Percentage of pupation,
It is 65.63 ± 6.32% by the ratio that larvae development is pupa；Eclosion rate, i.e., by pupa turn into adult ratio be 96.88 ±
3.13%；It is 63.57 ± 4.72% by the ratio that egg development is adult.
Count the quantity of female insect or male insect obtained in every batch of, and calculate accordingly each batch female insect or
The ratio of male insect.
For experimental result as shown in figure 5, the ratio of female insect is 53.92 ± 1.91%, the ratio of male insect is 46.08
± 1.91%, it is very nearly the same with the actual female in field and male ratio.
Claims (7)
1. the method for single head raising South America tomato leaf miner, which is characterized in that the method includes in transparent raising box, with guarantor
The step of fresh tomato leaf is host, and South America tomato leaf miner is raised by ovum to adult.
2. the method for single head raising South America tomato leaf miner according to claim 1, which is characterized in that fresh-keeping kind
Eggplant blade is made of method comprising the following steps: to be saturated the petiole that the absorbent cotton of water suction swathes tomato leaf.
3. the method for single head raising South America tomato leaf miner according to claim 2, which is characterized in that swathed with absorbent cotton
After the petiole, then with preservative film swathe the absorbent cotton；Absorbent cotton is folded up 180 ° from end 1/3~1/2, again
The absorbent cotton is swathed with preservative film；Mediate the upper end of the preservative film and the joint of the petiole.
4. the method for single head raising South America tomato leaf miner according to claim 2, which is characterized in that the length of the petiole
Degree is 45-50mm；The length of the absorbent cotton is 80-100mm, width is 30-40mm, with a thickness of 2.5-3.5mm.
5. the method for single head raising South America tomato leaf miner according to claim 1, which is characterized in that the transparent raising
The shape of box is cylinder, and the transparent raising box includes the upper raising box and lower raising box of mounted on top, the upper raising
Box is equal with the lower diameter for raising box, highly, and the upper raising box and the lower raising box are connected to up and down.
6. the method for single head raising South America tomato leaf miner according to claim 5, which is characterized in that the upper raising
It is equipped with opening on box, the lower bottom surface for raising box, the upper raising box and the lower raising box pass through the open communication.
7. the method for single head raising South America tomato leaf miner according to claim 5, which is characterized in that the raising box
Diameter is 80-90mm, is highly 20-30mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910834842.XA CN110432231B (en) | 2019-09-05 | 2019-09-05 | Method for breeding Nanmei tomato leaf miner with single head |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910834842.XA CN110432231B (en) | 2019-09-05 | 2019-09-05 | Method for breeding Nanmei tomato leaf miner with single head |
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| CN110432231A true CN110432231A (en) | 2019-11-12 |
| CN110432231B CN110432231B (en) | 2021-05-25 |
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| CN110432231B (en) | 2021-05-25 |
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