CN110192598A - A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies - Google Patents

A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies Download PDF

Info

Publication number
CN110192598A
CN110192598A CN201910295075.XA CN201910295075A CN110192598A CN 110192598 A CN110192598 A CN 110192598A CN 201910295075 A CN201910295075 A CN 201910295075A CN 110192598 A CN110192598 A CN 110192598A
Authority
CN
China
Prior art keywords
parts
black soldier
soldier flies
preparation
microorganism mix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910295075.XA
Other languages
Chinese (zh)
Inventor
余家华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sichuan Zhongdi Oil Technology Co Ltd
Original Assignee
Sichuan Zhongdi Oil Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan Zhongdi Oil Technology Co Ltd filed Critical Sichuan Zhongdi Oil Technology Co Ltd
Priority to CN201910295075.XA priority Critical patent/CN110192598A/en
Publication of CN110192598A publication Critical patent/CN110192598A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/90Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/045Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • A61K35/741Probiotics
    • A61K35/742Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/73Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/734Crataegus (hawthorn)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Birds (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Biochemistry (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies.According to parts by weight, the microorganism mix preparation of the prevention black soldier flies disease includes following components: 9-11 parts of EM bacterium, 18-22 parts of bacillus subtilis, 9-11 parts of borneol, 7-9 parts of hawthorn, 1-3 parts of enzyme preparation, 7-9 parts of microbial nutrition agent.Microorganism mix preparation of the present invention by the cooperation of EM bacterium, bacillus subtilis, borneol, enzyme preparation and microbial nutrition agent, will not in polypide and fecula stool residual chemicals, lead to the residual of harmful substance;And C0D, the ammonia nitrogen of moisture in black soldier flies feed resource and frass are reduced, the influence of feed stink, the palatability, pH value and temperature that improve feed is reduced, is had the effect of to the good growth of black soldier flies polypide splendid.

Description

A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies
Technical field
The present invention relates to black soldier flies cultural technique fields, mix system more particularly to a kind of microorganism suitable for black soldier flies Agent and its use (preparation) method.
Background technique
With society and economic rapid development, requirement of the people to living environment is gradually increased, and insect protein is a kind of This source nutrient, rich in natural high-quality protein, 8 kinds of amino acid, unsaturated fatty acid, vitamin and calcium needed by human, iron, Nutrient necessary to the human bodies such as the several mineral materials such as zinc, potassium, copper, selenium and microelement.But at present in black soldier flies cultured insect In the industry, since industrially scalable metaplasia produces insect, black soldier flies insect stocking density is caused to increase, and lead to large-scale continued fever (not searching out whether continued fever is continued fever) and mycosis are concurrent, especially in annual 6-10 month.Currently, in related skill This is contained without effective means in art field, not only brings huge loss to raiser, also limits black soldier flies insect egg The expansion of white industry.
Therefore, how to provide it is a kind of can efficiently contain black soldier flies continued fever and mycotic microorganism mix preparation, be this The problem of field technical staff's urgent need to resolve.
Summary of the invention
In view of this, the present invention provides a kind of microorganism mix preparations and its application method suitable for black soldier flies, no But efficiently containment black soldier flies continued fever and mycosis, can also be effective energy saving.
In order to solve the above technical problems, this invention takes following technical solutions:
A kind of microorganism mix preparation suitable for black soldier flies, according to parts by weight, including following components: 9-11 parts of EM bacterium, withered Careless bacillus 18-22 parts, 9-11 parts of borneol, 8-10 parts of hawthorn, 1-3 parts of enzyme preparation, 7-9 parts of microbial nutrition agent.
Preferably, the microbial nutrition agent is humic acid 3-4 parts and alga fertilizer 4-5 parts.
The beneficial effect of this technical solution is: humic acid can keep the work of EM bacterium and bacillus subtilis with alga fertilizer Property, preferably improve the function and effect of EM bacterium and bacillus subtilis.
Preferably, the enzyme preparation is protease, lipase, amylase, carbohydrase, cellulase, zytase and sweet Reveal one of dextranase or a variety of mixing.
Meanwhile the present invention also provides a kind of application method of microorganism mix preparation suitable for black soldier flies, including it is following Step:
(1) each raw material are weighed according to the parts by weight of the microorganism mix preparation described above suitable for black soldier flies;
(2) borneol and the hawthorn are ground into 40-60 mesh powder, obtain mixed-powder;
(3) by mixed-powder obtained by step (2) and the EM bacterium, the bacillus subtilis, the enzyme preparation, the humic acid It is mixed with the alga fertilizer, obtains the mixed fodder of feeding black soldier flies.
Compared with prior art, the beneficial effect of above-mentioned technical proposal of the present invention is: passing through EM bacterium and withered grass in the present invention The collective effect of bacillus can reduce the C0D of moisture, ammonia nitrogen in black soldier flies feed resource and frass, and it is smelly to reduce feed Taste, improve feed palatability, pH value and temperature influence, especially can be prevented from mouldy because of fecula stool, lead to parasitosis Occur, have the advantages that it is efficient, safe, free from environmental pollution, therefore to the growth of black soldier flies polypide with good effect.This hair Borneol and hawthorn are also added into bright, borneol gas faint scent, bitter, Xin Liang have the effect of inducing resuscitation of having one's ideas straightened out, clearing away heat to and alleviating pain, main to use In heat reliving and toxin-eliminating, go the effects of screen improving eyesight, removing necrosis, promoting granulation, anticancer;Hawthorn is uncommon to Shigella, proteus, large intestine angstrom Bacterium, staphylococcus aureus etc. have stronger bacteriostasis.By borneol and hawthorn miscellaneous function, so that EM bacterium of the present invention and withered Careless bacillus can preferably prevent black soldier flies and delay pyreticosis and mycotic generation.
Specific embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit Determine the scope of the present invention.
Embodiment 1
Microorganism mix preparation suitable for black soldier flies includes following components: 9 parts of EM bacterium, 18 parts of bacillus subtilis, borneol 9 Part, 8 parts of hawthorn, 1 part of cellulase, 3 parts of humic acid and 4 parts of alga fertilizer.
The preparation method of the above-mentioned microorganism mix preparation suitable for black soldier flies, comprising the following steps:
(1) parts by weight of the microorganism mix preparation suitable for black soldier flies as described above weigh each raw material;
(2) borneol and hawthorn are ground into 40-60 mesh powder, obtain mixed-powder;
(3) mixed-powder obtained by step (2) is mixed with EM bacterium, bacillus subtilis, cellulase, humic acid and alga fertilizer and is stirred It mixes, obtains the mixed fodder of feeding black soldier flies.
Embodiment 2
Microorganism mix preparation suitable for black soldier flies includes following components: 10 parts of EM bacterium, 20 parts of bacillus subtilis, borneol 10 Part, 9 parts of hawthorn, 2 parts of cellulase, 3.5 parts of humic acid and 4.5 parts of alga fertilizer.
The preparation method of the above-mentioned microorganism mix preparation suitable for black soldier flies, comprising the following steps:
(1) parts by weight of the microorganism mix preparation suitable for black soldier flies as described above weigh each raw material;
(2) borneol and hawthorn are ground into 40-60 mesh powder, obtain mixed-powder;
(3) mixed-powder obtained by step (2) is mixed with EM bacterium, bacillus subtilis, cellulase, humic acid and alga fertilizer and is stirred It mixes, obtains the mixed fodder of feeding black soldier flies.
Embodiment 3
Microorganism mix preparation suitable for black soldier flies includes following components: 11 parts of EM bacterium, 22 parts of bacillus subtilis, borneol 11 Part, 10 parts of hawthorn, 3 parts of cellulase, 4 parts of humic acid and 5 parts of alga fertilizer.
The preparation method of the above-mentioned microorganism mix preparation suitable for black soldier flies, comprising the following steps:
(1) parts by weight of the microorganism mix preparation suitable for black soldier flies as described above weigh each raw material;
(2) borneol and hawthorn are ground into 40-60 mesh powder, obtain mixed-powder;
(3) mixed-powder obtained by step (2) is mixed with EM bacterium, bacillus subtilis, cellulase, humic acid and alga fertilizer and is stirred It mixes, obtains the mixed fodder of feeding black soldier flies.
Below by microorganism mix preparation made from experimental verification in accordance with the above-mentioned embodiment 1-3 raw material proportioning, in reality Effect in the cultivation application of border black soldier flies.
By microorganism mix preparation obtained by above-described embodiment 1-3 in a manner of feed, by number it is corresponding feed in In the workshop rearing conditions identical Jianyang 1-3, every 200 disk of workshop, every disk launches 20,000 2 instar larvaes of black soldier flies.It launches 40 disks are extracted as sample, and June in every workshop after 10 days, and in July, it is primary to be extracted sample by above-mentioned steps respectively for September.In order to Test effect is preferably observed, while also setting up a reference examples, is randomly selected from other workshops and microorganism mixing system is not added 40 disks of agent carry out above-mentioned same operation, to protect the accuracy of test.Final result see the table below 1:
Table 1
By the data in upper table it should be apparent that the microorganism prepared with the raw material proportioning that 1-3 of the embodiment of the present invention is provided Mix preparation, in black soldier flies cultivation, compared to pair for not having addition microorganism mix preparation under the conditions of identical experimental test As usual, 2 black soldier flies of the embodiment of the present invention have more preferably growth result.
The above is only present pre-ferred embodiments, is not intended to limit the scope of the present invention, therefore Any subtle modifications, equivalent variations and modifications to the above embodiments according to the technical essence of the invention, still belong to Within the scope of technical solution of the present invention.

Claims (4)

1. a kind of microorganism mix preparation suitable for black soldier flies, which is characterized in that according to parts by weight, the prevention black soldier flies The microorganism mix preparation of disease includes following components: 9-11 parts of EM bacterium, 18-22 parts of bacillus subtilis, 9-11 parts of borneol, mountain 8-10 parts of short, bristly hair or beard, 1-3 parts of enzyme preparation, 7-9 parts of microbial nutrition agent.
2. a kind of microorganism mix preparation suitable for black soldier flies according to claim 1, which is characterized in that micro- life Object nutritional agents includes: 3-4 parts and alga fertilizer 4-5 parts of humic acid.
3. a kind of microorganism mix preparation suitable for black soldier flies according to claim 1, which is characterized in that the enzyme system Agent is one of protease, lipase, amylase, carbohydrase, cellulase, zytase and mannase or a variety of mixed It closes.
4. a kind of preparation method of the microorganism mix preparation suitable for black soldier flies, which comprises the following steps:
S1, each original is weighed according to the parts by weight for the microorganism mix preparation for being suitable for black soldier flies described in claim any one of 1-3 Material;
S2, the borneol and the hawthorn are ground into 40-60 mesh powder, obtain mixed-powder;
S3, by mixed-powder obtained by step s2 and the EM bacterium, the bacillus subtilis, the enzyme preparation, the humic acid It is mixed with the alga fertilizer, obtains a kind of microorganism mix preparation suitable for black soldier flies.
CN201910295075.XA 2019-04-12 2019-04-12 A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies Pending CN110192598A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910295075.XA CN110192598A (en) 2019-04-12 2019-04-12 A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910295075.XA CN110192598A (en) 2019-04-12 2019-04-12 A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies

Publications (1)

Publication Number Publication Date
CN110192598A true CN110192598A (en) 2019-09-03

Family

ID=67751889

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910295075.XA Pending CN110192598A (en) 2019-04-12 2019-04-12 A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies

Country Status (1)

Country Link
CN (1) CN110192598A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110839788A (en) * 2019-10-21 2020-02-28 广东海大集团股份有限公司 Special premix for black soldier fly breeding
CN111972358A (en) * 2020-08-24 2020-11-24 普罗欧生物科技(武汉)有限公司 Method for promoting oil accumulation of hermetia illucens insects
CN113616738A (en) * 2021-09-08 2021-11-09 贵州中地油科技有限公司 Pharmaceutical composition for treating hermetia illucens soft rot and preparation method thereof
CN114437988A (en) * 2022-02-28 2022-05-06 贵州医科大学 Cellulose degrading bacteria, microbial inoculum, preparation method and application thereof, and method for degrading vinasse

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105166358A (en) * 2015-08-26 2015-12-23 广东省昆虫研究所 Hermetia illucens L. larva feed additive and hermetia illucens L. larva forage
CN105558440A (en) * 2016-01-26 2016-05-11 华南农业大学 Tenebrio molitor and hermetia illucens feed based on organic refuse fermentation residues
CN107594234A (en) * 2017-10-27 2018-01-19 银川保绿特生物技术有限公司 It is a kind of to be used to cultivate biological composite interstitial substance of black soldier flies and preparation method thereof
CN107716508A (en) * 2017-09-19 2018-02-23 广东省生物资源应用研究所 A kind of method using black soldier flies bioconversion kitchen garbage
CN109090043A (en) * 2018-07-30 2018-12-28 开平市华声生物科技有限公司 A kind of black soldier flies larva cultural method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105166358A (en) * 2015-08-26 2015-12-23 广东省昆虫研究所 Hermetia illucens L. larva feed additive and hermetia illucens L. larva forage
CN105558440A (en) * 2016-01-26 2016-05-11 华南农业大学 Tenebrio molitor and hermetia illucens feed based on organic refuse fermentation residues
CN107716508A (en) * 2017-09-19 2018-02-23 广东省生物资源应用研究所 A kind of method using black soldier flies bioconversion kitchen garbage
CN107594234A (en) * 2017-10-27 2018-01-19 银川保绿特生物技术有限公司 It is a kind of to be used to cultivate biological composite interstitial substance of black soldier flies and preparation method thereof
CN109090043A (en) * 2018-07-30 2018-12-28 开平市华声生物科技有限公司 A kind of black soldier flies larva cultural method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110839788A (en) * 2019-10-21 2020-02-28 广东海大集团股份有限公司 Special premix for black soldier fly breeding
CN111972358A (en) * 2020-08-24 2020-11-24 普罗欧生物科技(武汉)有限公司 Method for promoting oil accumulation of hermetia illucens insects
CN113616738A (en) * 2021-09-08 2021-11-09 贵州中地油科技有限公司 Pharmaceutical composition for treating hermetia illucens soft rot and preparation method thereof
CN114437988A (en) * 2022-02-28 2022-05-06 贵州医科大学 Cellulose degrading bacteria, microbial inoculum, preparation method and application thereof, and method for degrading vinasse
CN114437988B (en) * 2022-02-28 2023-05-23 贵州医科大学 Cellulose degrading bacteria and microbial inoculum, preparation method, application and method for degrading distillers' grains

Similar Documents

Publication Publication Date Title
CN110192598A (en) A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies
Madende et al. Fish by-product use as biostimulants: An overview of the current state of the art, including relevant legislation and regulations within the EU and USA
Mpanga et al. The form of N supply determines plant growth promotion by P-solubilizing microorganisms in maize
Srivastava et al. Amelioration of sodic soil for wheat cultivation using bioaugmented organic soil amendment
Stibilj et al. Enhanced selenium content in buckwheat (Fagopyrum esculentum Moench) and pumpkin (Cucurbita pepo L.) seeds by foliar fertilisation
CN107098784A (en) Biological organic fertilizer and preparation method thereof
JP2022522228A (en) Pasture treatment to promote and reduce carbon sequestration of livestock greenhouse gas emissions
Adetunji et al. Efficacy of crude and immobilizedenzymes from Bacillus licheniformis for production of biodegraded feather meal and their assessment on chickens
Metwally et al. The individual and interactive role of arbuscular mycorrhizal fungi and Trichoderma viride on growth, protein content, amino acids fractionation, and phosphatases enzyme activities of onion plants amended with fish waste
Nosheen et al. Nutritive value of canola (Brassica napus L.) as affected by plant growth promoting rhizobacteria
Mishra et al. Suitability of agricultural by-products as production medium for spore production by Beauveria bassiana HQ917687
CN105565989A (en) Bioorganic-inorganic composite fertilizer containing chitin and peat, and application thereof
CN104478586A (en) Selenium-rich microbial preparation prepared by fermentation of animal serum and preparation method of selenium-rich microbial preparation
Khan et al. Evaluation of phytase production by fish gut bacterium, Bacillus subtilis, for processing of Ipomea aquatica leaves as probable aquafeed ingredient
CN108865928A (en) A kind of bacillus subtilis and the method for being used to prepare fruit of negundo probiotics
Jalal et al. Agronomic biofortification and productivity of wheat with soil zinc and diazotrophic bacteria in tropical savannah
CN105110941A (en) Novel amino acid organic fertilizer
Mandal et al. Optimization of tannase production and improvement of nutritional quality of two potential low-priced plant feedstuffs under solid state fermentation by Pichia kudriavzevii isolated from fish gut
Schoebitz et al. Characterization of bioactive compounds in blueberry and their impact on soil properties in response to plant biostimulants
CN110294648A (en) A kind of Compound fertilizer and preparation method thereof improveing soil
CN103947825A (en) Pet feed additive and its production method
Mandal et al. Effect of boron and sulfur interaction on some important biological indices in an inceptisol
CN104839492A (en) Environment-friendly carp feed without fish meal
CN103613417B (en) A kind of Special bio-organic fertilizer for rice and preparation method thereof
CN105754873A (en) Pesticide and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20190903

RJ01 Rejection of invention patent application after publication