CN111972358A - Method for promoting oil accumulation of hermetia illucens insects - Google Patents
Method for promoting oil accumulation of hermetia illucens insects Download PDFInfo
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- CN111972358A CN111972358A CN202010855960.1A CN202010855960A CN111972358A CN 111972358 A CN111972358 A CN 111972358A CN 202010855960 A CN202010855960 A CN 202010855960A CN 111972358 A CN111972358 A CN 111972358A
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- larvae
- hermetia illucens
- black soldier
- soldier fly
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention provides a method for promoting oil accumulation of hermetia illucens insects, which comprises the following steps: adding a sugar source and EM bacterial liquid into the feeding raw materials, and mixing to obtain a mixed feed; adding the mixed feed into a hermetia illucens breeding pond, adding hermetia illucens larvae, and breeding the larvae until the larvae enter a prepupa stage to obtain a residue mixture; and separating the worm residue mixture to obtain the hermetia illucens larvae with high oil content. According to the invention, sugar is used as a supplementary carbon source, EM bacterial liquid is used as an auxiliary material, and the accumulation of the black soldier fly grease is promoted, so that the utilization value of the black soldier fly is improved.
Description
Technical Field
The invention relates to the technical field of insect breeding, in particular to a method for promoting oil accumulation of hermetia illucens insects.
Background
The Hermetia illucens (Hermetia illucens) belonging to Hermetia of Diptera, also called Hermetia illucens, can be used in combination with microorganisms to digest solid organic wastes such as kitchen waste, wet waste, feces of poultry, and waste crops. The black soldier fly larvae after solid waste digestion are rich in crude oil, the content of the crude oil is 35-40%, wherein the content of unsaturated fatty acids is 40-45%, the unsaturated fatty acids mainly comprise oleic acid, linoleic acid, arachidonic acid and the like, and the unsaturated fatty acids can be widely applied to the fields of medicine, health care, skin care, energy and the like after extraction, separation and purification. In order to further improve the content of the hermetia illucens oil and the application value of the hermetia illucens oil, it is necessary to research a method for improving the accumulation amount of the oil in the insect body.
Disclosure of Invention
In view of the above, the invention provides a method capable of improving the nutritional value and the economical efficiency of hermetia illucens and effectively promoting the oil accumulation of hermetia illucens insects.
The technical scheme of the invention is realized as follows: the invention provides a method for promoting oil accumulation of hermetia illucens insects, which comprises the following steps:
adding a sugar source and EM bacterial liquid into a feeding raw material, and mixing to obtain a mixed feed;
step two, adding the mixed feed obtained in the step one into a black soldier fly breeding pond, adding black soldier fly larvae, and breeding the larvae until the larvae enter a prepupa stage to obtain a residue mixture;
and step three, separating the worm residue mixture obtained in the step two to obtain the hermetia illucens larvae with high oil content.
On the basis of the above technical solution, preferably, in the step one, the sugar source: the mass ratio of the feeding raw materials is 1: (1-20).
On the basis of the above technical solution, preferably, the EM bacterial liquid: the mass ratio of the total amount of the sugar source to the feeding raw material is 1: (10-20).
On the basis of the above technical scheme, preferably, in the step one, the sugar source is one or a mixture of more of glucose, xylose, brown sugar, galactose, lactose and dextrin.
Still more preferably, in the first step, the feeding raw materials include kitchen waste, wet waste and poultry manure.
On the basis of the technical scheme, preferably, the feeding raw materials are crushed, and the fineness of the crushed feeding raw materials is 60 meshes.
On the basis of the technical scheme, the water content of the mixed feed is preferably 70-80%.
On the basis of the above technical scheme, preferably, in the second step, the black soldier fly larvae are five-day-old black soldier fly larvae.
On the basis of the above technical scheme, preferably, in step two, the mixed feed: the mass ratio of the hermetia illucens larvae is 1: (0.01-0.1).
The preparation method of the EM bacterial liquid comprises the following steps: according to EM solid original strain: brown sugar: uniformly mixing water in a ratio of 10:100:1000, sealing, fermenting at 35-37 ℃, shaking once every 1-2 days, fermenting for 5-7 days to obtain EM liquid strain, mixing the EM liquid strain: brown sugar: mixing water at a ratio of 1:1:10, sealing, maintaining the temperature at 35-37 deg.C, shaking once every 1-2 days, and fermenting for 5-7 days to obtain EM bacterial liquid.
Compared with the prior art, the method for promoting the accumulation of the black soldier fly insect grease has the following beneficial effects:
according to the method, the sugar source and the EM bacterial liquid are adopted to carry out compound treatment on the feeding raw materials, the feeding raw materials are more favorable for the growth of the hermetia illucens by adopting a specific proportion, and more nutritional ingredients of the hermetia illucens larvae can be converted into grease.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Example 1
Smashing the kitchen waste until the fineness of the kitchen waste reaches 60 meshes, taking glucose as a sugar source, carrying out compound mixing on the glucose and the smashed kitchen waste according to the mass ratio of 1:1, and mixing the EM bacterial liquid and the compound mixed materials according to the mass ratio of 1:10 evenly mixing to obtain mixed feed with the water content of 70%, adding the mixed feed and 5-day-old hermetia illucens larvae into a hermetia illucens breeding pond according to the mass ratio of 1:0.01, keeping the temperature in the breeding pond at 30-35 ℃ and the relative humidity of air at 70-85% until the larvae enter a prepupation stage, and screening the hermetia illucens larvae.
Example 2
Smashing the kitchen waste until the fineness of the kitchen waste reaches 60 meshes, taking a mixture of xylose and brown sugar as a sugar source, wherein the mass ratio of the xylose to the brown sugar is 1:1, mixing the mixed sugar and the smashed kitchen waste according to the ratio of 1:10, compounding and mixing the EM bacterial liquid and the compounded and mixed materials according to the mass ratio of 1:15, uniformly mixing to obtain a mixed feed with the water content of 75%, adding the mixed feed and 5-day-old hermetia illucens larvae into a hermetia illucens breeding pond according to the mass ratio of 1:0.03, keeping the temperature in the breeding pond at 30-35 ℃ and the relative humidity of air at 70-85% until the larvae enter a prepupation stage, and screening the hermetia illucens larvae.
Example 3
Crushing the wet garbage until the fineness of the wet garbage reaches 60 meshes, taking a mixture of xylose and lactose as a sugar source, wherein the mass ratio of the xylose to the lactose is 2: 1, mixing, compounding and mixing the mixed sugar and the crushed wet garbage according to a mass ratio of 1:5, and mixing the EM bacterial liquid and the compounded and mixed materials according to a mass ratio of 1:10 evenly mixing to obtain mixed feed with the water content of 75%, adding the mixed feed and 5-day-old hermetia illucens larvae into a hermetia illucens breeding pond according to the mass ratio of 1:0.04, keeping the temperature in the breeding pond at 30-35 ℃ and the relative humidity of air at 70-85% until the larvae enter a prepupation stage, and screening the hermetia illucens larvae.
Example 4
Smashing the kitchen waste until the fineness of the kitchen waste reaches 60 meshes, taking the mixture of galactose and dextrin as a sugar source, and enabling the galactose and the dextrin to be 3 by mass: 1, mixing, compounding and mixing the mixed sugar and the crushed kitchen waste according to a mass ratio of 1:10, and mixing the EM bacterial liquid and the compounded and mixed materials according to a mass ratio of 1:20, uniformly mixing to obtain mixed feed with the water content of 70%, and mixing the mixed feed with 5-day-old hermetia illucens larvae according to the mass ratio of 1: adding into the Hermetia illucens breeding pond at a ratio of 0.03, maintaining the temperature in the breeding pond at 30-35 deg.C and the relative humidity of air at 70-85%, and sieving to obtain Hermetia illucens larvae when the larvae enter into pupa preportioning stage.
Example 5
Crushing the poultry manure until the fineness of the poultry manure reaches 60 meshes, taking a mixture of glucose and yellow sugar as a sugar source, wherein the mass ratio of the glucose to the yellow sugar is 1:1, mixing, compounding and mixing the mixed sugar and the crushed poultry manure according to a mass ratio of 1:20, and mixing the EM bacterial liquid and the compounded and mixed materials according to a mass ratio of 1:15, uniformly mixing to obtain a mixed feed with the water content of 80%, and mixing the mixed feed with 5-day-old hermetia illucens larvae according to the mass ratio of 1: adding into the Hermetia illucens breeding pond at a ratio of 0.1, maintaining the temperature in the breeding pond at 30-35 deg.C and the relative humidity of air at 70-85%, and sieving to obtain Hermetia illucens larvae when the larvae enter into pupa preportioning stage.
Comparative example
Smashing the kitchen waste until the fineness of the kitchen waste reaches 60 meshes, and enabling the smashed kitchen waste and 5-day-old hermetia illucens larvae to be 1 according to the mass ratio: adding into the Hermetia illucens breeding pond at a ratio of 0.03, maintaining the temperature in the breeding pond at 30-35 deg.C and the relative humidity of air at 70-85%, and sieving to obtain Hermetia illucens larvae when the larvae enter into pupa preportioning stage.
Cleaning the black soldier fly larvae obtained in examples 1-5 and comparative examples, respectively taking the same weight of black soldier fly larvae, mixing the black soldier fly larvae with methanol according to a ratio of 1:15(m/v), putting the mixture into a homogenizer, starting the homogenizer at a speed of 12000rpm for 5min to obtain a slurry-solvent mixture, putting the mixture into a centrifuge for centrifugal separation at a centrifugal rotation speed of 2000rpm for 5min, standing and layering the mixture after the centrifugation is finished, taking the uppermost methanol-oil layer, transferring the methanol-oil layer into a rotary evaporator for rotary evaporation in a vacuum state, weighing the oil weight when the methanol-oil layer is evaporated without solvent, and calculating the percentage of the oil weight in the larvae to obtain the following results:
the black soldier fly cultured by the method has good oil content.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. A method for promoting oil accumulation of hermetia illucens insects is characterized by comprising the following steps:
adding a sugar source and EM bacterial liquid into a feeding raw material, and mixing to obtain a mixed feed;
step two, adding the mixed feed obtained in the step one into a black soldier fly breeding pond, adding black soldier fly larvae, and breeding the larvae until the larvae enter a prepupa stage to obtain a residue mixture;
and step three, separating the worm residue mixture obtained in the step two to obtain the hermetia illucens larvae with high oil content.
2. The method of promoting oil accumulation in hermetia illucens according to claim 1, wherein in step one, the sugar source: the mass ratio of the feeding raw materials is 1: (1-20).
3. The method for promoting oil accumulation in hermetia illucens according to claim 1, wherein the EM bacterial liquid: the mass ratio of the total amount of the sugar source to the feeding raw material is 1: (10-20).
4. The method of claim 1, wherein in step one, the sugar source is one or a mixture of glucose, xylose, mannose, galactose, lactose and dextrin.
5. The method of promoting black soldier fly oil accumulation as claimed in claim 1, wherein in step one, said feeding materials comprise kitchen waste, wet waste and poultry manure.
6. The method for promoting the accumulation of black soldier fly insect grease as claimed in claim 1, wherein the feeding raw material is crushed, and the fineness of the crushed feeding raw material is 60 meshes.
7. The method of promoting oil accumulation in black soldier fly according to claim 1, wherein the mixed feed has a moisture content of 70-80%.
8. The method of promoting oil accumulation in black soldier fly insects of claim 1, wherein in step two, said black soldier fly larvae are five-day-old black soldier fly larvae.
9. The method for promoting the accumulation of black soldier fly insect oil according to claim 1, wherein in step two, the mixed feed: the mass ratio of the hermetia illucens larvae is 1: (0.01-0.1).
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104945028A (en) * | 2015-05-19 | 2015-09-30 | 刘金花 | Method for treating kitchen wastes by using hermetia illucens and organic fertilizer prepared by method |
| CN107594234A (en) * | 2017-10-27 | 2018-01-19 | 银川保绿特生物技术有限公司 | It is a kind of to be used to cultivate biological composite interstitial substance of black soldier flies and preparation method thereof |
| CN108684612A (en) * | 2017-04-10 | 2018-10-23 | 湖南水虻世家环保科技有限公司 | A method of handling fowl and animal excrement using black soldier flies |
| CN108703114A (en) * | 2018-04-28 | 2018-10-26 | 南安市创培电子科技有限公司 | A kind of black soldier flies environment-protecting cultivation method |
| CN109090043A (en) * | 2018-07-30 | 2018-12-28 | 开平市华声生物科技有限公司 | A kind of black soldier flies larva cultural method |
| US20190048367A1 (en) * | 2017-08-14 | 2019-02-14 | Lakeside Power and Methane, LLC | Biogas production from excrement |
| CN110192598A (en) * | 2019-04-12 | 2019-09-03 | 四川中地油科技有限公司 | A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies |
| CN111066733A (en) * | 2019-12-17 | 2020-04-28 | 李路胜 | Method for breeding hermetia illucens by preparing feed from organic perishable garbage |
-
2020
- 2020-08-24 CN CN202010855960.1A patent/CN111972358A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104945028A (en) * | 2015-05-19 | 2015-09-30 | 刘金花 | Method for treating kitchen wastes by using hermetia illucens and organic fertilizer prepared by method |
| CN108684612A (en) * | 2017-04-10 | 2018-10-23 | 湖南水虻世家环保科技有限公司 | A method of handling fowl and animal excrement using black soldier flies |
| US20190048367A1 (en) * | 2017-08-14 | 2019-02-14 | Lakeside Power and Methane, LLC | Biogas production from excrement |
| CN107594234A (en) * | 2017-10-27 | 2018-01-19 | 银川保绿特生物技术有限公司 | It is a kind of to be used to cultivate biological composite interstitial substance of black soldier flies and preparation method thereof |
| CN108703114A (en) * | 2018-04-28 | 2018-10-26 | 南安市创培电子科技有限公司 | A kind of black soldier flies environment-protecting cultivation method |
| CN109090043A (en) * | 2018-07-30 | 2018-12-28 | 开平市华声生物科技有限公司 | A kind of black soldier flies larva cultural method |
| CN110192598A (en) * | 2019-04-12 | 2019-09-03 | 四川中地油科技有限公司 | A kind of microorganism mix preparation and preparation method thereof suitable for black soldier flies |
| CN111066733A (en) * | 2019-12-17 | 2020-04-28 | 李路胜 | Method for breeding hermetia illucens by preparing feed from organic perishable garbage |
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Effective date of registration: 20220926 Address after: Next to Dongwang Primary School, Donggang Village, Dushan Town, Echeng District, Ezhou City, Hubei Province 436000 Applicant after: Ezhou Prooubohui Venture Capital Partnership (L.P.) Address before: 430000 south of Gaoxin 2nd Road and east of Guanggu 7th Road, Donghu New Technology Development Zone, Wuhan City, Hubei Province Applicant before: PROO Biotechnology (Wuhan) Co.,Ltd. |
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Effective date of registration: 20221031 Address after: 436000 Donggang Primary School, Donggang Village, Dushan Town, Echeng District, Ezhou City, Hubei Province Applicant after: Proo Biotechnology (Hubei) Co.,Ltd. (general partnership) Address before: Next to Dongwang Primary School, Donggang Village, Dushan Town, Echeng District, Ezhou City, Hubei Province 436000 Applicant before: Ezhou Prooubohui Venture Capital Partnership (L.P.) |
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Application publication date: 20201124 |