CN101011420B - Preparing process and usage of grease of housefly larva and fatty acid thereof - Google Patents
Preparing process and usage of grease of housefly larva and fatty acid thereof Download PDFInfo
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- CN101011420B CN101011420B CN2007100669684A CN200710066968A CN101011420B CN 101011420 B CN101011420 B CN 101011420B CN 2007100669684 A CN2007100669684 A CN 2007100669684A CN 200710066968 A CN200710066968 A CN 200710066968A CN 101011420 B CN101011420 B CN 101011420B
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- fly
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- fatty acid
- neutral lipase
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Abstract
The invention relates to a method for preparing fly oil and relative fatty acid, which comprises that washing the fly water, under 0.01MPa, drying it until the water content of fly is lower than 15%, breaking, adding organic solvent, extracting, separating solid and liquid, recycling the liquid to recycle the solvent to obtain the fly oil; in the buffer liquid prepared from sodium hydrogen phosphate and acid sodium phosphate at 6.0-8.0pH, adding neutral lipase, to reach 180-200 lipase active amount in each milliliter; adding fly oil into the buffer liquid at 1:0.5-1.5 volume ratio, mixing, reacting, stopping reacting, laying and separating, separating the oil, washing with the water in same volume, rotating and drying the oil, to obtain the fly fatty acid. The inventive fly oil and relative fatty acid can be used to treat liver cancer, lung cancer, or the like.
Description
Technical field
The invention belongs to technical field of bioengineering, relate in particular to the preparation method and the purposes of a kind of fly maggot oil and fatty acid thereof.
Background technology
At present, fly larvae does not carry out the extraction of fly maggot oil as the raw material of high protein feed, with the fly larvae drying, beat powder, promptly sells as feedstuff.
And the existing production technology one of fatty acid is to be raw material with the deep sea fish oil, and through hydrolysis, separation and purification again obtains fatty acid mixed, and wherein the content of unsaturated fatty acid is higher.Another kind of technology is to be raw material with the vegetable oil, and through hydrolysis, separation and purification again obtains fatty acid mixed.Deep sea fish oil is the fatty acid of raw material production, and is higher because of the content of unsaturated fatty acid wherein, has vessel softening, strengthens blood supply, promotes function such as brain development, thereby can be used as health product, also can be used as the medicine for the treatment of cardiovascular disease.The fatty acid function that with the vegetable oil is raw material production is general, cardiovascular system of human body is had certain function of health care, but only can seldom be developed as health product as food usefulness, and more application is in the day chemical industry.
Summary of the invention
The preparation method and the purposes that the purpose of this invention is to provide a kind of fly maggot oil and fatty acid thereof.
Fly maggot oil and fatty acid preparation method thereof are that fly larvae water is cleaned, below vacuum 0.01Mpa, 30～40 ℃ of temperature are dried in the fly larvae water content below 15%, pulverize, the organic solvent that adds 1～10 times of its weight, 0～50 ℃ of lixiviate 1～5 hour, it was biphase to separate solid-liquid, solvent is reclaimed in liquid phase 20～40 ℃ of evaporations below 0.01Mpa vacuum, obtains fly maggot oil; In disodium-hydrogen and sodium dihydrogen phosphate pH6.0～8.0 buffer with the water configuration, add neutral lipase, reach every milliliter 180～200 enzyme iu alive; In being furnished with the buffer of neutral lipase, add fly maggot oil, the volume ratio of buffer and fly maggot oil is 1: 0.5～1.5, keep 38～40 ℃, stirring reaction 1.5～3.5 hours, stopped reaction, static separation, tell oil phase, with equal-volume water washing 2～5 times, oil phase is 30～40 ℃ of rotation evaporates to dryness below vacuum 0.01Mpa, get the fly larvae fatty acid.
Described organic solvent is one or both a mixed solvent of petroleum ether or hexane.The weight ratio of petroleum ether, hexane and mixed solvent thereof and fly maggot powder is 1: 1～10.Neutral lipase is Pancreas Sus domestica neutral lipase, yeast neutral lipase and antibacterial neutral lipase.
Fly maggot oil and fatty acid thereof are used to prepare the medicine of treatment hepatocarcinoma, pulmonary carcinoma, gastric cancer, leukemia, breast carcinoma, ovarian cancer or cervical cancer.
The present invention is fly maggot oil and the fatty acid thereof that feedstock production obtains with the fly larvae, and animal experiment shows nontoxic, and steady quality is difficult for oxidation deterioration, easily is absorbed by the body.Show that through national new drug center test seven kinds of cancers of hepatocarcinoma, pulmonary carcinoma, gastric cancer, leukemia, breast carcinoma, ovarian cancer, cervical cancer of people are had the good curing effect.
The specific embodiment
Number of patent application is that the step of 200510060277.4 fly maggot breeding method is as follows:
1) chrysomyia megacephala (big head golden fly) is raised
Adopt transparent mode fly room, roof, draw in the room and be provided with many ropes and have a rest for kind of flies, chrysomyia megacephala (big head golden fly) is a food with animal proteinum, brown sugar, fruit, with Herba Epimedii, lysine, methionine, vitamin as urging the ovum element, collect fly blow with collection ovum plate, on collection ovum dish, put the carbonic acid ammonia preparation Testa Tritici of son one very much, lay eggs to lure kind of fly to concentrate;
2) fly larvae is raised
Adopt airy fly larvae room, the roof transparent material, ventilation fan and fan are housed in the room, window, seal with screen window is tight in the place that air hole and room are interlinked to the outside, place a plurality of maggot culturing pools in the fly larvae room, in maggot culturing pool, put into maggot bait, put into above-mentioned fly blow simultaneously, 28 ℃～60 ℃ of maggot culturing pool temperature controls, maggot culturing pool is by light-burning magnesium powder 30%～50%, magnesium oxide 30%～50%, wood slag 15%～25% raw material is made, the components in weight percent of maggot bait is a Rana nigromaculata 15%～30%, fresh-water fishes 15%～30%, Cordyceps 0.1%～0.2%, Ganoderma powder 1%～2%, chicken 20%～30%, fruit 25%～35%, all the other are other plant feed, pulverize the back and ferment in fermentation vat 4～6 days.
Embodiment 1
With number of patent application is that the fly larvae that 200510060277.4 cultural method is cultured is a raw material, fly larvae water is cleaned, under vacuum 0.01Mpa, temperature is dried to water content 10% in the fly larvae for 40 ℃, pulverizes, and adds the petroleum ether of 1 times of its weight, 0 ℃ of lixiviate 5 hours, it is biphase to separate solid-liquid, and petroleum ether is reclaimed in liquid phase 20 ℃ of evaporations under 0.01Mpa vacuum, obtains fly maggot oil; In disodium-hydrogen and sodium dihydrogen phosphate pH6.0 buffer with the water configuration, add the Pancreas Sus domestica neutral lipase, reach every milliliter 180 enzyme iu alive; In the buffer of being furnished with the Pancreas Sus domestica neutral lipase, add fly maggot oil, the volume ratio of buffer and fly maggot oil is 1: 0.5, keep 38 ℃, stirring reaction 1.5 hours, stopped reaction, static separation, tell oil phase, with equal-volume water washing 2 times, oil phase is 40 ℃ of rotation evaporates to dryness under vacuum 0.01Mpa, get the fly larvae fatty acid.
Embodiment 2
With number of patent application is that the fly larvae that 200510060277.4 cultural method is cultured is a raw material, fly larvae water is cleaned, at vacuum 0.003Mpa, temperature is dried to water content 13% in the fly larvae for 30 ℃, pulverizes, and adds the hexane of 10 times of its weight, 50 ℃ of lixiviates 1 hour, it is biphase to separate solid-liquid, and hexane is reclaimed in liquid phase 30 ℃ of evaporations under 0.003Mpa vacuum, obtains fly maggot oil; In disodium-hydrogen and sodium dihydrogen phosphate pH8.0 buffer with the water configuration, add the yeast neutral lipase, reach every milliliter 200 enzyme iu alive; In the buffer of allocating the yeast neutral lipase into, add fly maggot oil, the volume ratio of buffer and fly maggot oil is 1: 1.0, keep 40 ℃, stirring reaction 3.5 hours, stopped reaction, static separation, tell oil phase, with equal-volume water washing 5 times, oil phase is 30 ℃ of rotation evaporates to dryness under vacuum 0.003Mpa, get the fly larvae fatty acid.
Embodiment 3
With number of patent application is that the fly larvae that 200510060277.4 cultural method is cultured is a raw material, fly larvae water is cleaned, under vacuum 0.008Mpa, temperature is dried to water content 15% in the fly larvae for 35 ℃, pulverizes, add the petroleum ether of 7 times of its weight and the mixed solvent of hexane, petroleum ether is 1: 1 with the mixed solvent ratio of hexane, and 20 ℃ of lixiviates 4 hours, it was biphase to separate solid-liquid, liquid phase is 40 ℃ of mixed solvents that evaporate recovery petroleum ether and hexane under 0.008Mpa vacuum, obtain fly maggot oil; In disodium-hydrogen and sodium dihydrogen phosphate pH7.0 buffer with the water configuration, add the antibacterial neutral lipase, reach every milliliter 195 enzyme iu alive; In the buffer of being furnished with the antibacterial neutral lipase, add fly maggot oil, the volume ratio of buffer and fly maggot oil is 1: 1.5, keep 39 ℃, stirring reaction 3 hours, stopped reaction, static separation, tell oil phase, with equal-volume water washing 3 times, oil phase is 35 ℃ of rotation evaporates to dryness under vacuum 0.008Mpa, get the fly larvae fatty acid.
Embodiment 4
With number of patent application is that the fly larvae that 200510060277.4 cultural method is cultured is a raw material, fly larvae water is cleaned, under vacuum 0.005Mpa, temperature is dried to water content 11% in the fly larvae for 40 ℃, pulverizes, add the petroleum ether of 3 times of its weight and the mixed solvent of hexane, petroleum ether is 1: 10 with the mixed solvent ratio of hexane, and 40 ℃ of lixiviates 2 hours, it was biphase to separate solid-liquid, liquid phase is 35 ℃ of mixed solvents that evaporate recovery petroleum ether and hexane under 0.005Mpa vacuum, obtain fly maggot oil; In disodium-hydrogen and sodium dihydrogen phosphate pH7.5 buffer with the water configuration, add the Pancreas Sus domestica neutral lipase, reach every milliliter 190 enzyme iu alive; In the buffer of allocating the Pancreas Sus domestica neutral lipase into, add fly maggot oil, the volume ratio of buffer and fly maggot oil is 1: 1.3, keep 40 ℃, stirring reaction 2 hours, stopped reaction, static separation, tell oil phase, with equal-volume water washing 4 times, oil phase is 37 ℃ of rotation evaporates to dryness under vacuum 0.005Mpa, get the fly larvae fatty acid.
Claims (1)
1. fly maggot oil and fatty acid preparation method thereof, be characterised in that, fly larvae water is cleaned, and below vacuum 0.01Mpa, 30～40 ℃ of temperature are dried in the fly larvae water content below 15%, pulverize, the organic solvent that adds 1～10 times of its weight, 0～50 ℃ of lixiviate 1～5 hour, it was biphase to separate solid-liquid, solvent is reclaimed in liquid phase 20～40 ℃ of evaporations below 0.01Mpa vacuum, obtains fly maggot oil; In disodium-hydrogen and sodium dihydrogen phosphate pH6.0～8.0 buffer with the water configuration, add neutral lipase, reach every milliliter 180～200 enzyme iu alive; In being furnished with the buffer of neutral lipase, add fly maggot oil, the volume ratio of buffer and fly maggot oil is 1: 0.5～1.5, keep 38～40 ℃, stirring reaction 1.5～3.5 hours, stopped reaction, static separation, tell oil phase, with equal-volume water washing 2～5 times, oil phase is 30～40 ℃ of rotation evaporates to dryness below vacuum 0.01Mpa, get the fly larvae fatty acid; Described organic solvent is one or both a mixed solvent of petroleum ether or hexane; Described neutral lipase is Pancreas Sus domestica neutral lipase, yeast neutral lipase or antibacterial neutral lipase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100669684A CN101011420B (en) | 2007-01-29 | 2007-01-29 | Preparing process and usage of grease of housefly larva and fatty acid thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100669684A CN101011420B (en) | 2007-01-29 | 2007-01-29 | Preparing process and usage of grease of housefly larva and fatty acid thereof |
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| CN101011420A CN101011420A (en) | 2007-08-08 |
| CN101011420B true CN101011420B (en) | 2011-04-06 |
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| CN2007100669684A Expired - Fee Related CN101011420B (en) | 2007-01-29 | 2007-01-29 | Preparing process and usage of grease of housefly larva and fatty acid thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102796611A (en) * | 2012-07-17 | 2012-11-28 | 浙江省长三角循环经济技术研究院 | Soaking extraction process for maggot oil |
| CN102827689A (en) * | 2012-07-17 | 2012-12-19 | 浙江省长三角循环经济技术研究院 | Maggot oil preparation process |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101805672B (en) * | 2010-03-08 | 2012-01-25 | 中山大学 | Biodiesel and preparation method thereof |
| CN101880591A (en) * | 2010-06-13 | 2010-11-10 | 中山大学 | Application of housefly maggots as oil material insects |
| CN101880590A (en) * | 2010-06-13 | 2010-11-10 | 中山大学 | Application of Chrysomya megacephala larvae as oil insects |
| CN101880592A (en) * | 2010-06-13 | 2010-11-10 | 中山大学 | Use of Boettcherisca peregrine maggots as oil plant insects |
| CN105878113A (en) * | 2016-05-18 | 2016-08-24 | 中山市尤利卡天然药物有限公司 | External preparation capable of preventing and treating skin inflammations and protecting skins |
| CN108392496B (en) * | 2018-04-09 | 2020-06-26 | 大连麦琪克生物技术有限公司 | Extraction process of traditional Chinese medicine maggot fatty acid |
| CN108587770A (en) * | 2018-05-15 | 2018-09-28 | 山东省药学科学院 | A kind of extraction process of elder brother's fatty oil |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1297691A (en) * | 1999-12-01 | 2001-06-06 | 中国科学院动物研究所 | Separation and extraction process of effective component in resource insect |
| CN1869180A (en) * | 2006-06-26 | 2006-11-29 | 王爱民 | Method of extracting fly maggot oil |
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2007
- 2007-01-29 CN CN2007100669684A patent/CN101011420B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297691A (en) * | 1999-12-01 | 2001-06-06 | 中国科学院动物研究所 | Separation and extraction process of effective component in resource insect |
| CN1869180A (en) * | 2006-06-26 | 2006-11-29 | 王爱民 | Method of extracting fly maggot oil |
Non-Patent Citations (4)
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| 曹国民，等.脂肪酶催化动物脂肪水解.中国油脂22 2.1997,22(2),第30页左栏"2.6 放大实验"部分，第29页右栏第1行. |
| 曹国民,等.脂肪酶催化动物脂肪水解.中国油脂22 2.1997,22(2),第30页左栏"2.6 放大实验"部分,第29页右栏第1行. * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102796611A (en) * | 2012-07-17 | 2012-11-28 | 浙江省长三角循环经济技术研究院 | Soaking extraction process for maggot oil |
| CN102827689A (en) * | 2012-07-17 | 2012-12-19 | 浙江省长三角循环经济技术研究院 | Maggot oil preparation process |
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| CN101011420A (en) | 2007-08-08 |
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