CN108901615A - Utilize the method for trollflower pharmacological property culture medium culture cordyceps mycelium - Google Patents
Utilize the method for trollflower pharmacological property culture medium culture cordyceps mycelium Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/06—Products with modified nutritive value, e.g. with modified starch content
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
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Abstract
The invention discloses a kind of methods using trollflower pharmacological property culture medium culture cordyceps mycelium, mainly include：Step 1 will mix extraction with 10-15 times of volume distilled water after trollflower drying and crushing, obtain trollflower Aqueous extracts；Gained trollflower Aqueous extracts are added in basal medium step 2 according to the ratio of mass percent 3-8%, obtain pharmacological property culture medium；Cordyceps militaris link bacterial strain is inoculated into the pharmacological property culture medium and carries out fermented and cultured by step 3；Fermentation materials are centrifuged after step 4, fermented and cultured, supernatant is collected and obtains Cordyceps militaris fermentation liquid, collection precipitates up to the cordyceps mycelium.By the way that a certain amount of trollflower Aqueous extracts are added in culture medium of Cordyceps militaris, it can be improved the content of cordycepin in cordyceps mycelium, by measurement, cordycepin content can reach 14.14mg/g or more in gained cordyceps mycelium in the present invention, its content is at least 2 times or more of blank control group, while being significantly increased to the fungistatic effect of cordyceps mycelium.
Description
Technical field
The present invention relates to Cordyceps militaris fermented and cultured and applied technical fields, it is more particularly related to a kind of utilization
The method of trollflower pharmacological property culture medium culture cordyceps mycelium.
Background technique
Cordyceps militaris also known as northern Chinese caterpillar Fungus, Cordceps militaris etc., are Ascomycota, meat seat mesh, Clavicipitaceae, Cordyceps
Type sepecies are a kind of cordyceps sinensis manually cultivated, identical as natural cordyceps sinensis, can equally adjust whole body function, improve and exempt from
Epidemic disease ability mainly has and protects the nourishing the liver of lung kidney-nourishing, anti-aging, antibacterial, anti-inflammatory, fatigue-resisting function.
Cordyceps militaris is to generate multiple nutritional components based on artificial nutrition and artificial environment.Through Chinese Academy of Sciences's physics and chemistry point
Test laboratory chemical examination report is analysed, there are the representative 4 kinds of main pharmacological components of cordyceps sinensis：Cordycepin, cordycepic acid, Cordyceps sinensis polysaccharide and
SOD enzyme, artificial Chinese caterpillar fungus is compared with wild cordyceps, other than cordycepic acid is slightly below wild Chinese caterpillar fungus, remaining obviously higher than
Wild Chinese caterpillar fungus.Especially in numerous Cordyceps, the cordycepin of most peculiar representative ingredient can be generated, artificial cordyceps are unique
The kind of a large amount of cordycepins can be generated.The content of cordycepin is probably 10 times of wild Chinese caterpillar fungus or so in artificial Chinese caterpillar fungus, and worm
Careless element plays crucial effect as the representative ingredient of Cordyceps militaris in terms of playing medicinal effects.Therefore, people use at present
Various ways improve the content of cordycepin in Cordyceps militaris, generally improve cordycepin using modes such as mutagenic species, Optimal Mediums
Content, and Optimal Medium be it is the most frequently used be also a kind of simplest mode.
Therefore it provides a kind of excellent culture medium and growing environment of suitable Cordyceps militaris production, simultaneously to raising cordycepin content
Make cordycepin play medical effect to have great importance.
Summary of the invention
It is an object of the invention to solve at least the above problems, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of sides using trollflower pharmacological property culture medium culture cordyceps mycelium
Method can be improved the content of cordycepin in the fermented rear mycelium of Cordyceps militaris thallus.
It is a still further object of the present invention to provide a kind of Cordyceps militaris mycelia obtained using trollflower pharmacological property culture medium culture
The Cordyceps militaris chiffon cake of body preparation, can be improved the quality of chiffon cake.
In order to realize these purposes and other advantages according to the present invention, a kind of utilization trollflower pharmacological property culture medium is provided
The method for cultivating cordyceps mycelium, wherein include the following steps：
Step 1 will mix extraction with 10-15 times of volume distilled water after trollflower drying and crushing, obtain trollflower Aqueous extracts；
Gained trollflower Aqueous extracts are added in basal medium step 2 according to the ratio of mass percent 3-8%, obtain
To pharmacological property culture medium；Wherein, the basal medium mainly comprises the following components in parts by weight：150-250 parts of potato, wheat bran
15-25 parts, 10-20 parts of niblet, 15-25 parts of glucose, 3-8 parts of peptone and 800-1200 parts of distilled water；
Cordyceps militaris link bacterial strain is inoculated into the pharmacological property culture medium and carries out fermented and cultured by step 3, and inoculum concentration is volume ratio 3-
8%, fermentation total time is 7-9 days；
Fermentation materials are centrifuged after step 4, fermented and cultured, supernatant is collected and obtains Cordyceps militaris fermentation
Liquid, collection precipitate up to the cordyceps mycelium.
Preferably, the basal medium further includes 1-3 parts by weight microelement composition, the trace and REE elements
Object consists of the following parts by weight：Potassium dihydrogen phosphate 0.5-0.8, ferrous sulfate 0.2-0.3, magnesium sulfate 0.3-0.8, sulfuric acid
Zinc 0.2-0.4 and calcium carbonate 0.8-1.2 and vitamin B10.001-0.0015。
Preferably, fermentation culture method is temperature gradient cultivation in step 3, specially：First stage cultivates 3 days,
Cultivation temperature is incremented to 20 degrees Celsius from first day from 18 degrees Celsius, and every 20-24 hours is incremented by 1 degree Celsius；Second stage training
It supports 2 days, cultivation temperature is 20-22 degrees Celsius；Phase III cultivates 2-4 days, and cultivation temperature is 27-28 degrees Celsius.
Preferably, the preparation method of trollflower Aqueous extracts is specially temperature gradient extraction method, including following step in step 1
Suddenly：
Step 1.1 mixes the trollflower after drying and crushing with the distilled water of 5-7 times of volume, and temperature keeps 42-45 Celsius
Degree, extraction time are 2-3 hours, are separated by filtration and respectively obtain the first extracting solution and first dregs of a decoction；
Step 1.2 mixes first dregs of a decoction with the distilled water of 3-5 times of volume, and temperature is kept for 60-65 degrees Celsius, when extraction
Between be 4-6 hours, be separated by filtration to obtain the second extracting solution and second dregs of a decoction；
Step 1.3 mixes second dregs of a decoction with 2-3 times of volume distilled water, boils 10-15 minutes, is separated by filtration to obtain
Three extracting solutions and the third dregs of a decoction；
Step 1.4 mentions the mixing of the first extracting solution, the second extracting solution and third extracting solution to get the trollflower water
Liquid.
Preferably, for fermented and cultured in the process every the 20-30 minutes light stimulations of progress in 3-4 hours, illumination is strong in step 3
Degree is the lux 200-300.
Preferably, in step 4 gained cordyceps mycelium -18 degrees Celsius or less freezing 36-50 hours, then into
Row freeze-drying is to constant weight, and the cordyceps mycelium after drying is crushed to obtain by cordyceps mycelium after being dried
Cordyceps mycelium dry powder.
Preferably, trollflower Aqueous extracts are added in basal medium according to the ratio of mass percent 6%.
A kind of Cordyceps militaris chiffon cake prepared using cordyceps mycelium as claimed in claim 6, comprising following heavy
Measure the component of part：Egg 40-60, flour 15-20, fruiting bodies of cordyceps militaris powder 2-3, Cordyceps militaris bacterium solution 10-12, edible oil 7-12,
Sugared 10-30 and lemon juice 1-5.
The present invention is include at least the following beneficial effects：
The present invention can be improved cordyceps mycelium by the way that a certain amount of trollflower Aqueous extracts are added in culture medium of Cordyceps militaris
The content of middle cordycepin, by measurement, cordycepin content can reach in gained cordyceps mycelium in the present invention
14.1470mg/g or more, content are at least 2 times or more of blank control group, while to the fungistatic effect of cordyceps mycelium
It is significantly increased.
The present invention is applied to relative wind egg by the cordyceps mycelium and cordyceps sinensis fermentation liquor that obtain the method culture
In cake preparation, milk, and improved formulations and technique are substituted completely with cordyceps sinensis fermentation liquor, it is each to improve chiffon cake nutrition, mouthfeel etc.
Item quality, and in the case where not adding any preservative, shelf-life of the chiffon cake under 20-30 degrees Celsius of room temperature prolongs
Length was to 10-18 days.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention is described in further detail below, to enable those skilled in the art's refer to the instruction text being capable of evidence
To implement.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other elements or combinations thereof.
The present invention provides a kind of method using trollflower pharmacological property culture medium culture cordyceps mycelium, wherein including with
Lower step：
Step 1 will mix extraction with 10-15 times of volume distilled water after trollflower drying and crushing, obtain trollflower Aqueous extracts；
Gained trollflower Aqueous extracts are added in basal medium step 2 according to the ratio of mass percent 3-8%, obtain
To pharmacological property culture medium；Wherein, the basal medium mainly comprises the following components in parts by weight：150-250 parts of potato, wheat bran
15-25 parts, 10-20 parts of niblet, 15-25 parts of glucose, 3-8 parts of peptone and 800-1200 parts of distilled water.
Cordyceps militaris link bacterial strain is inoculated into the pharmacological property culture medium and carries out fermented and cultured by step 3, and inoculum concentration is volume ratio 3-
8%, fermentation total time is 7-9 days.
Fermentation materials are centrifuged after step 4, fermented and cultured, supernatant is collected and obtains Cordyceps militaris fermentation
Liquid, collection precipitate up to the cordyceps mycelium.
In above scheme, by the way that a certain amount of trollflower Aqueous extracts are added in culture medium of Cordyceps militaris, cordyceps are improved
The content of cordycepin in filament, by measurement, cordycepin content can reach in gained cordyceps mycelium in the present invention
14.1470mg/g, content are the 2 times or more of blank control group, while being improved to the fungistatic effect of cordyceps mycelium.
In one preferred embodiment, the basal medium further includes 1-3 parts by weight microelement composition, the micro member
Promotor composition consists of the following parts by weight：Potassium dihydrogen phosphate 0.5-0.8, ferrous sulfate 0.2-0.3, magnesium sulfate 0.3-
0.8, zinc sulfate 0.2-0.4 and calcium carbonate 0.8-1.2 and vitamin B10.001-0.0015。
In the program, the yield of cordyceps mycelium can be improved by adding microelement composition.
In one preferred embodiment, fermentation culture method is temperature gradient cultivation in step 3, specially：First stage training
It supports 3 days, cultivation temperature is incremented to 20 degrees Celsius from first day from 18 degrees Celsius, and every 20-24 hours is incremented by 1 degree Celsius；Second
Stage cultivates 2 days, and cultivation temperature is 20-22 degrees Celsius；Phase III cultivates 2-4 days, and cultivation temperature is 27-28 degrees Celsius.
In the program, by changing traditional constant temperature incubation method, using gradient Fluctuation temperature culture method, in third cultivation stage bacterium
Filament yield dramatically increases, relative to room temperature cultural method output increased 8.3%.
In one preferred embodiment, the preparation method of trollflower Aqueous extracts is specially temperature gradient extraction method in step 1, including
Following steps：
Step 1.1 mixes the trollflower after drying and crushing with the distilled water of 5-7 times of volume, and temperature keeps 42-45 Celsius
Degree, extraction time are 2-3 hours, are separated by filtration and respectively obtain the first extracting solution and first dregs of a decoction；
Step 1.2 mixes first dregs of a decoction with the distilled water of 3-5 times of volume, and temperature is kept for 60-65 degrees Celsius, when extraction
Between be 4-6 hours, be separated by filtration to obtain the second extracting solution and second dregs of a decoction；
Step 1.3 mixes second dregs of a decoction with 2-3 times of volume distilled water, boils 10-15 minutes, is separated by filtration to obtain
Three extracting solutions and the third dregs of a decoction；
Step 1.4 mentions the mixing of the first extracting solution, the second extracting solution and third extracting solution to get the trollflower water
Liquid.
It is raw in trollflower Aqueous extracts relative to the method routinely boiled by temperature gradient step by step arithmetic method in the program
Alkaloids content, flavonoids bioactive substance content significantly improve, and bioactive substance recovery rate improves 12%.
In one preferred embodiment, every the 20-30 minutes light stimulations of progress in 3-4 hours during fermented and cultured in step 3,
Intensity of illumination is the lux 200-300.
In one preferred embodiment, in step 4 gained cordyceps mycelium -18 degrees Celsius or less freezing 36-50 hours,
Then it is freeze-dried the cordyceps mycelium to constant weight, after being dried, the cordyceps mycelium after drying is subjected to powder
It is broken to obtain cordyceps mycelium dry powder.
In one preferred embodiment, trollflower Aqueous extracts are added in basal medium according to the ratio of mass percent 6%.
A kind of Cordyceps militaris chiffon cake prepared using the cordyceps mycelium, is contained the following parts by weight：Chicken
Egg 40-60, flour 15-20, fruiting bodies of cordyceps militaris powder 2-3, Cordyceps militaris bacterium solution 10-12, edible oil 7-12, sugar 10-30 and lemon
Lemon juice 1-5.
In the program, by with trollflower pharmacological property culture medium fermentation output cordyceps mycelium and cordyceps sinensis fermentation liquor application
Into chiffon cake preparation, milk, and improved formulations and technique are substituted completely with cordyceps sinensis fermentation liquor, improves chiffon cake well
The items quality such as nutrition, mouthfeel, and in the case where not adding any preservative, the chiffon cake is Celsius in room temperature 20-30
Extended shelf-life under degree was to 10-18 days.
Embodiment 1
1) extraction will be mixed with 10 times of volume distilled water after 50 parts of trollflower drying and crushings, obtains trollflower Aqueous extracts；
2) basal medium is prepared according to following parts by weight of component：150 parts of potato, 15 parts of wheat bran, 10 parts of niblet, grape
15 parts of sugar, 3 parts of peptone and 1000 parts of distilled water, institute is added into basal medium according to the ratio of mass percent 3%
Trollflower Aqueous extracts are obtained, pharmacological property culture medium is obtained；
3) 100 milliliters of dosing culture medium in each triangular flask, sealed membrane sealing, labelled high pressure steam sterilization 121
DEG C, 20 minutes.Cooling move back accesses Cordyceps militaris fermenting microbe by 3% inoculum concentration into every bottle of superclean bench, moves into 27 DEG C of shaking table,
150rpm is cultivated 7 days.Cordyceps militaris fermentation liquid and cordyceps mycelium are respectively obtained after fermentation.By calculating,
Mycelium content is 1.00g/ml.
4) cordycepin content measures
4.1 sample pretreatment
Take out the fermentation liquid in shaking table, be transferred to centrifuge tube, abandon supernatant after 4000r/min centrifugation 15min, precipitating spend from
Sub- water washing 3 times is put into ultra low temperature freezer and freezes 48 hours, moves into freeze drier and dried to constant weight later, weighed.
4.2 extracting
The extraction of cordycepin uses ultrasonic extraction, carries out preliminary experiment first to determine the power of ultrasonic wave.After taking drying
Cordyceps mycelium, by material-water ratio 1：30 are added distilled water, extract 20min at ultrasonic power 400w, 4000r/min from
The heart 15 minutes, precipitating is abandoned, supernatant is taken, obtains sample solution.
The measurement of 4.3 cordycepin contents
4.3.1 the determination of cordycepin standard curve
0.5mg cordycepin standard items are weighed, distilled water is added and is settled to 100ml, is configured to the mother liquor of 0.5mg/L, then
The cordycepin solution for preparing 0,1.0,2.0,3.0,4.0,5.0mg/L respectively again, measures its absorbance under the wavelength of 259nm
(A), with cordycepin concentration (C) for abscissa, it is that ordinate draws standard curve with absorbance (A), obtains normal equation：Y=
0.0878x+2.2837 (R2=0.9998).
4.3.2 in sample cordycepin content determination
It takes sample solution 1ml to dilute 100 times, measures absorbance when 259nm, bring normal equation into and find out concentration C.1ml
The amount S of cordycepin in sample0=C × V0×10(V0=1ml), the amount S=S of cordycepin in every part of sample0× V, cordycepin content
The amount (S) of s=cordycepin/mycelia weight (M).(note：Unit conversion should be carried out by bringing into when data calculate)
Measuring cordycepin content in mycelium is 7.62mg/g.
Embodiment 2
1) extraction will be mixed with 15 times of volume distilled water after 50 parts of trollflower drying and crushings, obtains trollflower Aqueous extracts；
2) basal medium is prepared according to following parts by weight of component：250 parts of potato, 25 parts of wheat bran, 20 parts of niblet, grape
25 parts of sugar, 8 parts of peptone and 1200 parts of distilled water, institute is added into basal medium according to the ratio of mass percent 7%
Trollflower Aqueous extracts are obtained, pharmacological property culture medium is obtained；
3) 100 milliliters of dosing culture medium in each triangular flask, sealed membrane sealing, labelled high pressure steam sterilization 121
DEG C, 20 minutes.Cooling move back accesses Cordyceps militaris fermenting microbe by 3% inoculum concentration into every bottle of superclean bench, moves into 27 DEG C of shaking table,
150rpm is cultivated 8 days.Cordyceps militaris fermentation liquid and cordyceps mycelium are respectively obtained after fermentation.By calculating,
Mycelium content is 0.62g/ml.
4) cordycepin content measuring method such as embodiment 1, measuring cordycepin content in mycelium is 14.14mg/g.
1 trollflower Aqueous extracts additive amount of table changes statistical form to cordyceps mycelium yield and cordycepin content
By trollflower Aqueous extracts additive amount it is respectively the parallel test of 1-8, and experimental data is counted, statistics knot
Fruit is shown in Table 1, it can be seen that trollflower Aqueous extracts have certain inhibiting effect to cordyceps mycelium biomass, in certain model
In enclosing, trollflower adds concentration and increases, and the trend successively decreased is presented in the biomass of cordyceps mycelium, but the trend successively decreased gradually is delayed.
Meanwhile trollflower has facilitation to the content of cordycepin, to the facilitation of cordycepin when trollflower additive amount is less than 3%
Unobvious, i.e. the incrementss of cordycepin content are few；Zooming trend is presented in the content of cordycepin after 3%, becomes again after 5%
In gentle, at 7%, biomass reaches 14.14mg/g, is increased more than one times compared to the control group；Although illustrating adding for trollflower
Adding has certain inhibiting effect to the growth of Cordyceps militaris, but can promote the promotion of cordycepin content in Cordyceps militaris.
Embodiment 3
1) extraction will be mixed with 15 times of volume distilled water after 50 parts of trollflower drying and crushings, obtains trollflower Aqueous extracts；
2) basal medium is prepared according to following parts by weight of component：250 parts of potato, 25 parts of wheat bran, 20 parts of niblet, grape
25 parts of sugar, 8 parts of peptone, 2 parts of microelement composition and 1200 parts of distilled water；Wherein, the microelement composition by
The component of following parts by weight is constituted：Potassium dihydrogen phosphate 0.5, ferrous sulfate 0.2, magnesium sulfate 0.3, zinc sulfate 0.2 and calcium carbonate
0.8 and vitamin B10.001.Gained trollflower water is added into basal medium according to the ratio of mass percent 6% to mention
Liquid obtains pharmacological property culture medium；
3) 100 milliliters of dosing culture medium in each triangular flask, sealed membrane sealing, labelled high pressure steam sterilization 121
DEG C, 20 minutes.It is cooling to move back into every bottle of inoculation cordyceps militaris link bacterial strain 3% of superclean bench, 27 DEG C of shaking table are moved into, 150rpm culture 7
It.Cordyceps militaris fermentation liquid and cordyceps mycelium are respectively obtained after fermentation.By calculating, mycelium content is
1.52g/ml。
4) cordycepin content measuring method such as embodiment 1, measuring cordycepin content in mycelium is 14.34mg/g.
The addition of microelement has positive influence to mycelium content and cordycepin content, especially to mycelial life
Object amount effect is obvious.
Embodiment 4
1) extraction will be mixed with 15 times of volume distilled water after 50 parts of trollflower drying and crushings, obtains trollflower Aqueous extracts；
2) basal medium is prepared according to following parts by weight of component：250 parts of potato, 25 parts of wheat bran, 20 parts of niblet, grape
25 parts of sugar, 8 parts of peptone and 1200 parts of distilled water；Institute is added into basal medium according to the ratio of mass percent 6%
Trollflower Aqueous extracts are obtained, pharmacological property culture medium is obtained.
3) 100 milliliters of dosing culture medium in each triangular flask, sealed membrane sealing, labelled high pressure steam sterilization 121
DEG C, 20 minutes.Cooling is moved back into every bottle of inoculation cordyceps militaris link bacterial strain 3% of superclean bench, and temperature gradient cultivation is cultivated, always
Fermentation time 9 days, specially：First stage cultivates 3 days, and it is Celsius that cultivation temperature was incremented to 20 from 18 degrees Celsius from first day
Degree is incremented by 1 degree Celsius in every 24 hours；Second stage culture 2 days, cultivation temperature was 22 degrees Celsius；Phase III cultivates 4 days, training
Supporting temperature is 28 degrees Celsius.Cordyceps militaris fermentation liquid and cordyceps mycelium are respectively obtained after fermentation.Pass through meter
It calculates, mycelium content is 1.98g/ml.
4) cordycepin content measuring method such as embodiment 1, measuring cordycepin content in mycelium is 13.98mg/g
Temperature gradient cultivation can significantly improve mycelial biomass, but influence on cordycepin content little.
Embodiment 5
1) extraction will be mixed with 15 times of volume distilled water after 50 parts of trollflower drying and crushings, obtains trollflower Aqueous extracts；
2) basal medium is prepared according to following parts by weight of component：200 parts of potato, 20 parts of wheat bran, 20 parts of niblet, grape
25 parts of sugar, 8 parts of peptone, 3 parts of microelement composition and 1000 parts of distilled water；Wherein, the microelement composition by
The component of following parts by weight is constituted：Potassium dihydrogen phosphate 0.6, ferrous sulfate 0.2, magnesium sulfate 0.5, zinc sulfate 0.4 and calcium carbonate
0.9 and vitamin B10.0015.Gained trollflower water is added into basal medium according to the ratio of mass percent 6% to mention
Liquid obtains pharmacological property culture medium.
3) 100 milliliters of dosing culture medium in each triangular flask, sealed membrane sealing, labelled high pressure steam sterilization 121
DEG C, 20 minutes.Cooling is moved back into every bottle of inoculation cordyceps militaris link bacterial strain 3% of superclean bench, and temperature gradient cultivation is cultivated, always
Fermentation time 9 days, specially：First stage cultivates 3 days, and it is Celsius that cultivation temperature was incremented to 20 from 18 degrees Celsius from first day
Degree is incremented by 1 degree Celsius in every 24 hours；Second stage culture 2 days, cultivation temperature was 22 degrees Celsius；Phase III cultivates 4 days, training
Supporting temperature is 28 degrees Celsius.Cordyceps militaris fermentation liquid and cordyceps mycelium are respectively obtained after fermentation.Pass through meter
It calculates, mycelium content is 2.02g/ml.
4) cordycepin content measuring method such as embodiment 1, measuring cordycepin content in mycelium is 14.34mg/g.
In addition, obvious in antibacterial aspect effect using the cordyceps mycelium of the trollflower pharmacological property culture medium culture.
Inspection bacterium is Escherichia coli, staphylococcus aureus, three kinds of bacillus subtilis.Spread plate method is detected.
Plating medium contains the following parts by weight：10 parts of peptone, 3 parts of beef extract, 5 parts of sodium chloride, agar 20
Part, 1000 parts of distilled water.
The preparation of cordycepin aqueous solution
1) it is divided into the control group cordyceps mycelium for being not added with trollflower Aqueous extracts and trollflower water is added by different proportion
The test group cordyceps mycelium of extract；
The drying of cordyceps mycelium：It is put into cultured cordyceps mycelium in dried culture dish, uses
Sealed membrane, which is wrapped, is placed on ultra low temperature freezer freezing for 24 hours, then is placed in freeze drier dry 48h, weighing record.
The extraction of cordycepin：Dry control group and experimental group cordyceps mycelium are distinguished into grind into powder, with 1：30
Material-water ratio add water, grinding is placed in ultrasonic cell disruptor ultrasonic, sets ultrasonic power 400w, ultrasonic time 20 divides
Clock, ultrasonic 5s, interval 5s.After ultrasound, it is centrifuged 15min in 4000rpm, supernatant is taken, respectively obtains control group and experiment
Group cordycepin aqueous solution, then measures absorbance under ultraviolet specrophotometer 259nm wavelength, and calculate concentration, spare.
The processing of cordycepin aqueous solution：Spare cordycepin aqueous solution will be extracted on superclean bench with complete
It is spare in the small centrifuge tube to sterilize in advance at being put into after autoclaved water system membrane filtration removal microorganism.
2) bacteriostatic test is carried out using paper disk method：
A, control group cordycepin aqueous solution is diluted 20,40,60,80,100 times respectively, test group cordycepin aqueous solution with
The amount for adding trollflower Aqueous extracts is variable, if 1%, 3%, 5%, 7% 4 gradient.
B, with the scraps of paper of tweezers clamping sterilizing, 50 μ L cordycepin Aqueous extracts is added on each scraps of paper with liquid-transfering gun, are placed
In averagely placing three scraps of paper on the plate for coating bacterium, on each plate, it is inverted 35 in constant incubator after being sealed with sealed membrane
DEG C constant temperature incubation, cultivate 12h and for 24 hours when record the size of inhibition zone respectively.
Interpretation of result
Various concentration cordycepin is to the bacteriostasis of three kinds of bacterium after the dilution of control group cordycepin aqueous solution, as shown in table 2 below.
Bacteriostasis of the 2 various concentration cordycepin of table to three kinds of bacterium
Note：Scraps of paper diameter is 1.1cm, and upper table data are inhibition zone overall diameter-scraps of paper diameter, measurement method ten
Word interior extrapolation method；0% is blank control, and 100% is undiluted cordycepin aqueous solution.
It is obtained from table 2, concentration is 16.55mg/L before cordycepin aqueous solution dilutes, and adds cordycepin compared with blank control
There is obvious inhibition zone to occur, for different inspection bacterium, biocidal property is different, but the fungistatic effect under not diluted original liquid concentration
Most preferably.
For bacillus subtilis, when cordycepin additive amount is 20%, there is more apparent inhibition zone, with
Biocidal property has obvious inhibition zone, but three compared with being not added with cordycepin when cordycepin concentration increases to 40%, 60%, 80%
Bacteriostasis no significant difference between concentration, when cordycepin concentration is 100%, fungistatic effect is best.
It is in increase as cordycepin concentration increases to 20%, 40%, 60% biocidal property also by 0% for Escherichia coli
Trend；When concentration is 60% and 80%, bacteriostasis no significant difference；When concentration is 100%, biocidal property is most strong.
For staphylococcus aureus, when cordycepin concentration is 0%, 20%, 40%, 60%, with concentration
Increase biocidal property to increase, when concentration is 60% to 80%, biocidal property no significant difference, fungistatic effect is best when concentration is 100%.
Cordycepin has differences three kinds of inspection bacterium biocidal properties, under the concentration before the dilution of cordycepin aqueous solution, to large intestine
The biocidal property of bacillus is most strong, most weak to the biocidal property of bacillus subtilis.Comprehensive Correlation cordycepin examines the antibacterial of bacterium to three kinds
Effect discovery, staphylococcus aureus is most sensitive to cordycepin, as larger inhibition zone, withered grass occurs at first in the increase of concentration
The sensibility of bacillus is taken second place, and in three kinds of inspection bacterium, staphylococcus aureus and bacillus subtilis are gram sun
Property bacterium, Escherichia coli are Gram-negative bacteria, this result illustrates that cordycepin Aqueous extracts are strong to the fungistatic effect of gram-positive bacteria
In Gram-negative bacteria, (Meng Zhaoli, Zhu Kai Polysaccharides in Cultured Cordyceps militaris is antibacterial and the research of antioxidation for the result and Meng Zhaoli etc.
[D] food research and development, 2008,29 (9)：Result of study 31-33.) is identical.
The cordycepin that generates is to the bacteriostasis of three kinds of bacterium after addition different proportion trollflower Aqueous extracts, as shown in table 3 below.
Bacteriostasis of the cordycepin generated after the addition different proportion trollflower Aqueous extracts of table 3 to three kinds of bacterium
The cordycepin when adding 0%, 1%, 3%, 5% and 7% trollflower Aqueous extracts is measured with ultraviolet specrophotometer
The concentration of aqueous solution is respectively：18.56mg/L, 24.79mg/L, 25.16mg/L, 52.51mg/L and 65.96mg/L.
Obtain from table 3, increased with trollflower Aqueous extracts addition concentration, the cordycepin biocidal property in cordyceps mycelium by
It is cumulative strong, it is stronger to three kinds of bacterium bacteriostasis when adding 3% trollflower Aqueous extracts, and when adding 3%, to hay bacillus
Reach maximum with the fungistatic effect of staphylococcus aureus；And the cordyceps sinensis when trollflower concentration is 1%, in cordyceps mycelium
Element is maximum to the bacteriostasis of Escherichia coli.It is comprehensively compared, the cordycepin after addition trollflower Aqueous extracts in cordyceps mycelium
Biocidal property be improved, but bacteriostasis increase is significantly staphylococcus aureus and Escherichia coli, and to withered grass bar
The influence of bacterium is smaller.
The test result of consolidated statement 2 and table 3 is available, and the addition of trollflower Aqueous extracts can be improved in cordyceps mycelium
Cordycepin fungistatic effect, it is especially more apparent to staphylococcus aureus and Escherichia coli bacteriostasis, when being not added with lily feet
When the cordycepin aqueous solution that flower Aqueous extracts extract is undiluted, the bacteriostatic diameter of Escherichia coli is 0.8cm, and trollflower Aqueous extracts add
When adding 1%, the bacteriostatic diameter of Escherichia coli is 1.3cm, and bacteriostasis is obviously reinforced；When be not added with trollflower Aqueous extracts extraction
When cordycepin aqueous solution is undiluted, the bacteriostatic diameter of staphylococcus aureus is 0.6cm, and when trollflower Aqueous extracts addition 3%,
The bacteriostatic diameter of staphylococcus aureus is 0.87cm, and bacteriostasis is reinforced.From lily feet spent culture medium culture cordyceps mycelium
Measurement of the cordycepin of middle extraction to hay bacillus, Escherichia coli and staphylococcus aureus bacteriostasis, can be close to function
Chinese medicine and microorganism between interaction provide fundamental basis, and for determine promote cordycepin biocidal property best Chinese medicine
Addition concentration provides data support.
Embodiment 6
Chiffon cake formula is：50 grams of egg, 15 grams of flour, 2 grams of fruiting bodies of cordyceps militaris powder, 10 grams of Cordyceps militaris bacterium solution and
7 grams of salad oil, 10 grams and 2 milliliters of lemon juice of white granulated sugar sugar.It is mixed after egg is dismissed with remaining ingredient, obtains chiffon cake
Then chiffon cake is put into oven with 145 DEG C of baking 50min of upper and lower fire by paste, 130 DEG C of preheating 15min of oven.Chiffon cake
20-30 degrees Celsius of lower shelf-life is 10-13 days.
Embodiment 7
Chiffon cake formula is：60 grams of egg, 20 grams of flour, 3 grams of fruiting bodies of cordyceps militaris powder, 12 grams of Cordyceps militaris bacterium solution and
12 grams of corn oil, 30 grams of soft white sugar and 3 milliliters of lemon juice.Baking method such as embodiment 6.It is protected under 20-30 degrees Celsius of chiffon cake
The matter phase is 10-13 days.
Embodiment 8
1, chiffon cake formula is：50 grams of egg, 17.6 grams of flour, 2.4 grams of fruiting bodies of cordyceps militaris powder, Cordyceps militaris bacterium solution 11
Gram, 9 grams of salad oil, 17 grams of soft white sugar and 2 milliliters of lemon juice.Wherein, Cordyceps militaris daughter bacteria liquid and fruiting bodies of cordyceps militaris powder are
It is obtained by the fermentation of pharmacological property culture medium or culture of addition trollflower Aqueous extracts.Baking method such as embodiment 6.Chiffon cake 20-30
Degree Celsius lower shelf-life is 15-18 days.Wherein, fruiting bodies of cordyceps militaris preparation method is：Cordyceps militaris solid state cultivation culture medium includes
The component of following parts by weight：Cooked rice 50, wheat bran 20, dried silkworm chrysalis meal 10, the trollflower dregs of a decoction 6, sawdust 6, magnesium sulfate 0.1, wheat flour
6, glucose 2 and water 120, pH6-7.The cultural method of fruiting bodies of cordyceps militaris is：By the Cordyceps militaris solid state cultivation culture medium
Bottling is inoculated with 25 DEG C of dark situation cultures after cordyceps mycelium to 1/2 full, sealing, 0.15MPa high pressure sterilization 2h, and mycelia is covered with
Move to bright place, 15 DEG C -25 DEG C are continued culture 25-40 days to there is orange red stroma to be formed and grown to 30-66mm high to get described
Fruiting bodies of cordyceps militaris is dried grinding, obtains fruiting bodies of cordyceps militaris powder.
Embodiment 9
Not plus the blank control group of fruiting bodies of cordyceps militaris powder and Cordyceps militaris bacterium solution.Baking method such as embodiment 6.20-30 takes the photograph
Family name's degree lower shelf-life is 3-7 days.
Chiffon cake is given 100 people to taste, establishing criteria evaluation table scores, successively to the color of chiffon cake
Pool, fragrance, appearance, fluffy degree, mouth feel score, every judging panel does not interfere with each other in scoring process.
The sensory evaluation criteria of 1 Cordyceps militaris relative wind chiffon cake of table
2 are shown in Table to the scoring situation of the relative wind chiffon cake according to table 1
2 relative wind chiffon cake of table scoring statistics
It is scored and is counted according to table 2, fruiting bodies of cordyceps militaris powder is added in chiffon cake formula and substitute milk with Cordyceps militaris bacterium solution
1/2/3 embodiment obtained by the comprehensive scores such as chiffon cake mouthfeel, color, fragrance and individual scores be superior to common relative wind egg
Cake, the shelf-life, also more common chiffon cake formula extended at least 3 days or more, wherein the sensory evaluation scores of scheme described in embodiment 3 are most
Height, close to full marks, the shelf-life, also longest, longest reached 18 days, were preferred plan.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and shown here as with embodiment.
Claims (8)
1. a kind of method using trollflower pharmacological property culture medium culture cordyceps mycelium, wherein include the following steps：
Step 1 will mix extraction with 10-15 times of volume distilled water after trollflower drying and crushing, obtain trollflower Aqueous extracts；
Gained trollflower Aqueous extracts are added in basal medium step 2 according to the ratio of mass percent 3-8%, obtain medicine
Property culture medium；Wherein, the basal medium mainly comprises the following components in parts by weight：150-250 parts of potato, wheat bran 15-25
Part, 10-20 parts of niblet, 15-25 parts of glucose, 3-8 parts of peptone and 800-1200 parts of distilled water；
Cordyceps militaris link bacterial strain is inoculated into the pharmacological property culture medium and carries out fermented and cultured by step 3, and inoculum concentration is volume ratio 3-8%,
Fermentation total time is 7-9 days；
Fermentation materials are centrifuged after step 4, fermented and cultured, supernatant is collected and obtains Cordyceps militaris fermentation liquid, receive
Collection precipitates up to the cordyceps mycelium.
2. utilizing the method for trollflower pharmacological property culture medium culture cordyceps mycelium as described in claim 1, wherein the base
Basal culture medium further includes 1-3 parts by weight microelement composition, the microelement composition by following parts by weight component structure
At：Potassium dihydrogen phosphate 0.5-0.8, ferrous sulfate 0.2-0.3, magnesium sulfate 0.3-0.8, zinc sulfate 0.2-0.4, calcium carbonate 0.8-
1.2 and vitamin B10.001-0.0015。
3. utilizing the method for trollflower pharmacological property culture medium culture cordyceps mycelium as described in claim 1, wherein step 3
Middle fermentation culture method is temperature gradient cultivation, specially：First stage cultivates 3 days, and cultivation temperature is from first day from 18
20 degrees Celsius degree Celsius are incremented to, every 20-24 hours is incremented by 1 degree Celsius；Second stage culture 2 days, cultivation temperature was taken the photograph for 20-22
Family name's degree；Phase III cultivates 2-4 days, and cultivation temperature is 27-28 degrees Celsius.
4. utilizing the method for trollflower pharmacological property culture medium culture cordyceps mycelium as described in claim 1, wherein step 1
The preparation method of middle trollflower Aqueous extracts is specially temperature gradient extraction method, is included the following steps：
Step 1.1 mixes the trollflower after drying and crushing with the distilled water of 5-7 times of volume, and temperature is kept for 42-45 degrees Celsius,
Extraction time is 2-3 hours, is separated by filtration and respectively obtains the first extracting solution and first dregs of a decoction；
Step 1.2 mixes first dregs of a decoction with the distilled water of 3-5 times of volume, and temperature is kept for 60-65 degrees Celsius, and extraction time is
It 4-6 hours, is separated by filtration to obtain the second extracting solution and second dregs of a decoction；
Step 1.3 mixes second dregs of a decoction with 2-3 times of volume distilled water, boils 10-15 minutes, is separated by filtration to obtain third and mentions
Take liquid and the third dregs of a decoction；
Step 1.4 mixes the first extracting solution, the second extracting solution and third extracting solution to get the trollflower Aqueous extracts.
5. utilizing the method for trollflower pharmacological property culture medium culture cordyceps mycelium as claimed in claim 3, wherein step 3
Every the 20-30 minutes light stimulations of progress in 3-4 hours during middle fermented and cultured, intensity of illumination is the lux 200-300.
6. utilizing the method for trollflower pharmacological property culture medium culture cordyceps mycelium as described in claim 1, wherein to step
In 4 gained cordyceps mycelium -18 degrees Celsius or less freezing 36-50 hours, be then freeze-dried to constant weight, done
Cordyceps mycelium after dry is crushed the cordyceps mycelium after drying to obtain cordyceps mycelium dry powder.
7. utilizing the method for trollflower pharmacological property culture medium culture cordyceps mycelium as described in claim 1, wherein trollflower
Aqueous extracts are added in basal medium according to the ratio of mass percent 6%.
8. a kind of Cordyceps militaris chiffon cake prepared using cordyceps mycelium as claimed in claim 6 includes following weight
The component of part：Egg 40-60, flour 15-20, fruiting bodies of cordyceps militaris powder 2-3, Cordyceps militaris bacterium solution 10-12, edible oil 7-12, sugar
10-30 and lemon juice 1-5.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355211A (en) * | 2018-12-13 | 2019-02-19 | 蒋涛 | A kind of Moringa Cordyceps militaris and its cultural method and bacteriostasis spray |
| CN111657490A (en) * | 2020-06-17 | 2020-09-15 | 北华大学 | Dandelion root saccharified liquid, dandelion root fermentation product, preparation methods of dandelion root saccharified liquid and dandelion root fermentation product, and product containing dandelion root saccharification liquid and dandelion root fermentation product |
| CN112136972A (en) * | 2019-06-28 | 2020-12-29 | 鲁东大学 | Method for producing feed additive by combined fermentation of cordyceps militaris and traditional Chinese medicines |
| CN113317119A (en) * | 2021-06-30 | 2021-08-31 | 山东中医药大学 | Method for improving cordycepin content by virtue of Jiuzhou cordyceps sinensis-sculellaria barbata bidirectional solid fermentation |
| CN115399192A (en) * | 2022-10-11 | 2022-11-29 | 翔宇药业股份有限公司 | Method for producing cordyceps militaris mycoplasm by using red skin blood replenishing oral liquid dregs and application of cordyceps militaris mycoplasm |
| CN116747176A (en) * | 2023-08-10 | 2023-09-15 | 珠海市华喜生物科技有限公司 | Lotus flower fermented product, preparation method and application thereof in anti-wrinkle tightening cosmetics |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1796539A (en) * | 2004-12-24 | 2006-07-05 | 青海月王青藏药业有限责任公司 | Ferment for producing aweto in large scale and technique for processing power of fungus |
| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN103215250A (en) * | 2013-03-30 | 2013-07-24 | 徐州鸿宇农业科技有限公司 | Protoplast mutation breeding method for improving cordycepin content of cordyceps militaris |
| CN103897991A (en) * | 2014-04-22 | 2014-07-02 | 杨秀芬 | Preparation method of solid active cordyceps sinensis mycelia isolated from culture media |
| CN106191173A (en) * | 2016-07-21 | 2016-12-07 | 内江师范学院 | A kind of method improving Cordyceps militaris fermenting and producing yield of Cordycepin |
| CN107410977A (en) * | 2017-05-23 | 2017-12-01 | 四川省亨润传承农业科技股份有限公司 | A kind of selenium-enriched food lozenge and preparation method using sweet potato stem leaf as raw material |
| CN108096091A (en) * | 2017-12-19 | 2018-06-01 | 广东东阳光药业有限公司 | Preventing baldness black hair composition and preparation method containing Cordyceps extract |
-
2018
- 2018-07-19 CN CN201810799016.1A patent/CN108901615A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1796539A (en) * | 2004-12-24 | 2006-07-05 | 青海月王青藏药业有限责任公司 | Ferment for producing aweto in large scale and technique for processing power of fungus |
| CN101897275A (en) * | 2010-08-13 | 2010-12-01 | 义乌市丹溪药用生物开发研究所 | Selenium-rich cordyceps culturing method |
| CN103215250A (en) * | 2013-03-30 | 2013-07-24 | 徐州鸿宇农业科技有限公司 | Protoplast mutation breeding method for improving cordycepin content of cordyceps militaris |
| CN103897991A (en) * | 2014-04-22 | 2014-07-02 | 杨秀芬 | Preparation method of solid active cordyceps sinensis mycelia isolated from culture media |
| CN106191173A (en) * | 2016-07-21 | 2016-12-07 | 内江师范学院 | A kind of method improving Cordyceps militaris fermenting and producing yield of Cordycepin |
| CN107410977A (en) * | 2017-05-23 | 2017-12-01 | 四川省亨润传承农业科技股份有限公司 | A kind of selenium-enriched food lozenge and preparation method using sweet potato stem leaf as raw material |
| CN108096091A (en) * | 2017-12-19 | 2018-06-01 | 广东东阳光药业有限公司 | Preventing baldness black hair composition and preparation method containing Cordyceps extract |
Non-Patent Citations (3)
| Title |
|---|
| 周会明等: "《食用菌栽培技术》", 31 May 2017, 北京：中国农业大学出版社 * |
| 朱蕴兰等: "蛹虫草面包的研制", 《食品科技》 * |
| 陈磊: "蛹虫草（Cordyceps militaris）生物学特性及发酵研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355211A (en) * | 2018-12-13 | 2019-02-19 | 蒋涛 | A kind of Moringa Cordyceps militaris and its cultural method and bacteriostasis spray |
| CN112136972A (en) * | 2019-06-28 | 2020-12-29 | 鲁东大学 | Method for producing feed additive by combined fermentation of cordyceps militaris and traditional Chinese medicines |
| CN111657490A (en) * | 2020-06-17 | 2020-09-15 | 北华大学 | Dandelion root saccharified liquid, dandelion root fermentation product, preparation methods of dandelion root saccharified liquid and dandelion root fermentation product, and product containing dandelion root saccharification liquid and dandelion root fermentation product |
| CN113317119A (en) * | 2021-06-30 | 2021-08-31 | 山东中医药大学 | Method for improving cordycepin content by virtue of Jiuzhou cordyceps sinensis-sculellaria barbata bidirectional solid fermentation |
| CN115399192A (en) * | 2022-10-11 | 2022-11-29 | 翔宇药业股份有限公司 | Method for producing cordyceps militaris mycoplasm by using red skin blood replenishing oral liquid dregs and application of cordyceps militaris mycoplasm |
| CN115399192B (en) * | 2022-10-11 | 2023-05-23 | 翔宇药业股份有限公司 | Method for producing cordyceps militaris mycoplasm by utilizing red-coated blood-replenishing oral liquid residues and application thereof |
| CN116747176A (en) * | 2023-08-10 | 2023-09-15 | 珠海市华喜生物科技有限公司 | Lotus flower fermented product, preparation method and application thereof in anti-wrinkle tightening cosmetics |
| CN116747176B (en) * | 2023-08-10 | 2024-10-29 | 珠海市华喜生物科技有限公司 | Lotus flower fermented product, preparation method and application thereof in anti-wrinkle tightening cosmetics |
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