CN109355211A - A kind of Moringa Cordyceps militaris and its cultural method and bacteriostasis spray - Google Patents
A kind of Moringa Cordyceps militaris and its cultural method and bacteriostasis spray Download PDFInfo
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- CN109355211A CN109355211A CN201811524569.2A CN201811524569A CN109355211A CN 109355211 A CN109355211 A CN 109355211A CN 201811524569 A CN201811524569 A CN 201811524569A CN 109355211 A CN109355211 A CN 109355211A
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- 241000220215 Moringa Species 0.000 title claims abstract description 60
- 235000011347 Moringa oleifera Nutrition 0.000 title claims abstract description 60
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000007921 spray Substances 0.000 title claims abstract description 23
- 239000001963 growth medium Substances 0.000 claims description 25
- 241000894006 Bacteria Species 0.000 claims description 23
- 230000035784 germination Effects 0.000 claims description 22
- 235000015097 nutrients Nutrition 0.000 claims description 17
- 238000005286 illumination Methods 0.000 claims description 13
- 241000190633 Cordyceps Species 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 239000011691 vitamin B1 Substances 0.000 claims description 7
- 239000011715 vitamin B12 Substances 0.000 claims description 7
- 241000255789 Bombyx mori Species 0.000 claims description 5
- 240000006162 Chenopodium quinoa Species 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 5
- 239000001393 triammonium citrate Substances 0.000 claims description 5
- 235000011046 triammonium citrate Nutrition 0.000 claims description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 230000000844 anti-bacterial effect Effects 0.000 claims description 4
- 238000005360 mashing Methods 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 229940088594 vitamin Drugs 0.000 claims description 4
- 229930003231 vitamin Natural products 0.000 claims description 4
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims description 2
- 239000011777 magnesium Substances 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- 238000010792 warming Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 1
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 abstract description 10
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 abstract description 10
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 abstract description 10
- 230000035876 healing Effects 0.000 abstract description 7
- 230000000144 pharmacologic effect Effects 0.000 abstract description 7
- 230000001408 fungistatic effect Effects 0.000 abstract description 6
- 239000000243 solution Substances 0.000 description 16
- 206010052428 Wound Diseases 0.000 description 9
- 208000027418 Wounds and injury Diseases 0.000 description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 6
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
- 235000019341 magnesium sulphate Nutrition 0.000 description 3
- 239000003595 mist Substances 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 241000382353 Pupa Species 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 229940124350 antibacterial drug Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 206010040872 skin infection Diseases 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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Abstract
The invention discloses a kind of cultural method of Moringa Cordyceps militaris, thus cultural method cultivate Moringa Cordyceps militaris and using the Moringa Cordyceps militaris prepare bacteriostasis spray, a kind of cultural method of Moringa Cordyceps militaris of the invention, step is simple, in the Moringa Cordyceps militaris cultivated, the content of cordycepin is high, there is good fungistatic effect by the bacteriostasis spray that the Moringa Cordyceps militaris is prepared, while the healing of wound surface can be effectively promoted, and pharmacological property does not stimulate mildly.
Description
Technical field
The present invention relates to a kind of Moringa Cordyceps militaris and its cultural methods and bacteriostasis spray.
Background technique
In modern clinical treatment, if dealt with improperly after treatment to wound surface, it is easy to cause wound infection, influence
The healing of wound.Therefore it needs to clean the surface of a wound after the treatment, cleaning is exactly the cuticula and skin for rotting skin infection
The tissue of lower fat deposit necrosis part is cleaned, and some antibacterial or antibacterial drug is then applied.But it is existing antibacterial or anti-
Bacterium drug significantly increases the pain of patient often with obvious irritation.
Now with studies have shown that cordyceps sinensis can not only inhibit the growth of bacterial micro-organism, simultaneously for being cured for wound surface
Closing also has facilitation, and the pharmacological property of cordyceps sinensis does not stimulate mildly, and cordycepin content is higher, and bacteriostasis is also better, still
In wild cordyceps sinensis, the content of cordycepin is lower, this constrains its medical value significantly, and the cordyceps sinensis of existing artificial culture
In, the content of cordycepin does not also have greatly improved.
Summary of the invention
The first purpose of the invention is to provide a kind of step is simple, and in the Moringa Cordyceps militaris cultivated, cordycepin content is high
Moringa Cordyceps militaris cultural method.
In order to achieve the above objectives, the technical solution adopted by the present invention is that:
A kind of cultural method of Moringa Cordyceps militaris comprising following steps:
(1) prepare nutrient solution: the nutrient solution is made by matching as follows: 20 ~ 25g of glucose, 1 ~ 1.5g of triammonium citrate, sulfuric acid
0.8 ~ 1.2g of magnesium, 1 ~ 5g of potassium dihydrogen phosphate, 10 ~ 30mg of vitamin, water 1000ml；
(2) it prepares culture medium: the nutrient solution obtained in butt and the step (1) is mixed in the ratio of 1:1.3 ~ 1.5
And stir evenly, culture medium is made, wherein the butt is by mass percentage, including following component: leaf of Moringa 10%, silkworm chrysalis
80%, quinoa 10%；
(3) it is inoculated with: strain being seeded in the step (2) in the culture medium obtained, and carries out sterilization treatment；
(4) bacterium germination: treated that the culture medium is put into carries out at bacterium germination in sterile darkroom by sterilized in the step (3)
Reason, keeping bacterium germination temperature is 18 ~ 19 DEG C；
(5) illumination: by the illumination for being 20 ~ 30 DEG C through bacterium germination treated the media transfer to temperature in the step (4)
It is cultivated 45 ~ 55 days under environment, until growing cordyceps mycelia；
(6) it dries: the culture medium for covering with the cordyceps mycelia in the step (5) is dried to get described in
Moringa Cordyceps militaris.
Preferably, in the step (1), the vitamin includes vitamin B1And vitamin B12, the vitamin B1
And vitamin B12Feed intake mass ratio be 1:1.
Preferably, in the step (2), the butt and the nutrient solution mixed proportion are 1:1.4.
Preferably, in the step (3), the time of sterilization treatment is 12h.
Preferably, in the step (4), the time of bacterium germination processing is 15 days.
Preferably, in the step (5), by the media transfer into light environment after, first holding illumination ring
The temperature in border is 25 DEG C, and is cultivated 45 days, and light environment is then warming up to 27 DEG C, continues culture 10 days.
Preferably, in the step (5), control intensity of illumination is 800lx, and light application time is 8 ~ 10h/ days.
Preferably, in the step (6), moisture content≤9% of the Moringa Cordyceps militaris after drying.
A second object of the present invention is to provide a kind of Moringa Cordyceps militaris as made from above-mentioned cultural method, the Moringa pupa
The cordycepin content of cordyceps sinensis is high.
Third object of the present invention is to provide a kind of bacteriostasis spray prepared using above-mentioned Moringa Cordyceps militaris, the antibacterial sprays
Agent has good fungistatic effect, while can effectively promote the healing of wound surface, and pharmacological property does not stimulate mildly.
In order to achieve the above objectives, the technical solution adopted by the present invention is that:
A kind of bacteriostasis spray, the bacteriostasis spray comprise the following steps: the Moringa Cordyceps militaris being added to the water crushing and is beaten
Slurry, hydrolysising protease is then added, 55 ~ 65 DEG C at a temperature of reaction is hydrolyzed, be spray-dried after fully reacting, general
The extract obtained after drying is added in aqueous solution and stirs evenly to arrive the bacteriostasis spray.
Due to the application of the above technical scheme, compared with the prior art, the invention has the following advantages: one kind of the invention
The cultural method of Moringa Cordyceps militaris, step is simple, and the content of cordycepin is high in the Moringa Cordyceps militaris cultivated, and passes through the Moringa pupa
The bacteriostasis spray that cordyceps sinensis is prepared has good fungistatic effect, while can effectively promote the healing of wound surface, and
Pharmacological property does not stimulate mildly.
Specific embodiment
Technical solution of the present invention is further elaborated combined with specific embodiments below.
Embodiment 1
A kind of cultural method of Moringa Cordyceps militaris comprising following steps:
(1) prepare nutrient solution: the nutrient solution is made by matching as follows: glucose 25g, triammonium citrate 1g, magnesium sulfate 1g, phosphoric acid
Potassium dihydrogen 3g, vitamin B110mg, vitamin B1210mg, water 1000ml.
(2) it prepares culture medium: being mixed and stirred for butt and nutrient solution uniformly, culture medium is made in the ratio of 1:1.4,
Middle butt include 10% leaf of Moringa, 80% silkworm chrysalis and 10% quinoa.
(3) it is inoculated with: strain being seeded in step (2) in culture medium obtained, and carries out sterilization treatment 12h.
(4) bacterium germination: treated that culture medium is put into that bacterium germination processing 15 is carried out in sterile darkroom by sterilized in step (3)
It, keeping bacterium germination temperature is 18 DEG C.
(5) illumination: will through bacterium germination treated media transfer to temperature be 25 DEG C in step (4), intensity of illumination is
It is cultivated 45 days under the light environment of 800lx, is then heated up to 27 DEG C, continue culture 10 days, until growing cordyceps mycelia.Here
Light application time be 9h/ days, and be intermittent irradiation.
(6) it dries: the culture medium for covering with cordyceps mycelia in step (5) is dried to get to Moringa Cordyceps militaris,
Here moisture content≤9% of the Moringa Cordyceps militaris after guaranteeing drying.
The Moringa Cordyceps militaris cultivated by the above method, through detecting, cordycepin content reaches 10mg/g, and potassium content reaches
0.5mg/g。
Bacteriostasis spray is prepared by the Moringa Cordyceps militaris, which comprises the following steps: Moringa Cordyceps militaris is added
Enter in water crush mashing, hydrolysising protease is then added, 60 DEG C at a temperature of reaction is hydrolyzed, sprayed after fully reacting
Mist is dry, the extract obtained after drying is added in aqueous solution and is stirred evenly to get bacteriostasis spray is arrived.
The bacteriostasis spray being prepared by Moringa Cordyceps militaris has good fungistatic effect, while can effectively promote
Into the healing of wound surface, and pharmacological property does not stimulate mildly.Through detecting, pH value 11.52 can improve blood of human body pH value,
95.94% is reached to the bacteriostasis rate of staphylococcus aureus, 96.09% is reached to the bacteriostasis rate of Escherichia coli, and stingless to skin
Swash property.
Embodiment 2
A kind of cultural method of Moringa Cordyceps militaris comprising following steps:
(1) prepare nutrient solution: the nutrient solution is made by matching as follows: glucose 20g, triammonium citrate 1.5g, magnesium sulfate 0.8g,
Potassium dihydrogen phosphate 5g, vitamin B15mg, vitamin B125mg, water 1000ml.
(2) it prepares culture medium: being mixed and stirred for butt and nutrient solution uniformly, culture medium is made in the ratio of 1:1.3,
Middle butt include 10% leaf of Moringa, 80% silkworm chrysalis and 10% quinoa.
(3) it is inoculated with: strain being seeded in step (2) in culture medium obtained, and carries out sterilization treatment 12h.
(4) bacterium germination: treated that culture medium is put into that bacterium germination processing 15 is carried out in sterile darkroom by sterilized in step (3)
It, keeping bacterium germination temperature is 19 DEG C.
(5) illumination: will through bacterium germination treated media transfer to temperature be 25 DEG C in step (4), intensity of illumination is
It is cultivated 45 days under the light environment of 800lx, is then heated up to 27 DEG C, continue culture 10 days, until growing cordyceps mycelia.Here
Light application time be 8h/ days, and be intermittent irradiation.
(6) it dries: the culture medium for covering with cordyceps mycelia in step (5) being dried to get Moringa Cordyceps militaris is arrived, and
Moisture content≤9% of Moringa Cordyceps militaris after guaranteeing drying.
The Moringa Cordyceps militaris cultivated by the above method, through detecting, cordycepin content reaches 11mg/g, and potassium content reaches
1mg/g。
Bacteriostasis spray is prepared by the Moringa Cordyceps militaris, which comprises the following steps: Moringa Cordyceps militaris is added
Enter in water crush mashing, hydrolysising protease is then added, 55 DEG C at a temperature of reaction is hydrolyzed, sprayed after fully reacting
Mist is dry, the extract obtained after drying is added in aqueous solution and is stirred evenly to get bacteriostasis spray is arrived.
The bacteriostasis spray being prepared by Moringa Cordyceps militaris has good fungistatic effect, while can effectively promote
Into the healing of wound surface, and pharmacological property does not stimulate mildly.Through detecting, pH value 11.53 can improve blood of human body pH value,
93.51% is reached to the bacteriostasis rate of staphylococcus aureus, 95.74% is reached to the bacteriostasis rate of Escherichia coli, and stingless to skin
Swash property.
Embodiment 3
A kind of cultural method of Moringa Cordyceps militaris comprising following steps:
(1) prepare nutrient solution: the nutrient solution is made by matching as follows: glucose 22g, triammonium citrate 1.2g, magnesium sulfate 1.2g,
Potassium dihydrogen phosphate 1g, vitamin B115mg, vitamin B1215mg, water 1000ml.
(2) it prepares culture medium: being mixed and stirred for butt and nutrient solution uniformly, culture medium is made in the ratio of 1:1.5,
Middle butt include 10% leaf of Moringa, 80% silkworm chrysalis and 10% quinoa.
(3) it is inoculated with: strain being seeded in step (2) in culture medium obtained, and carries out sterilization treatment 12h.
(4) bacterium germination: treated that culture medium is put into that bacterium germination processing 15 is carried out in sterile darkroom by sterilized in step (3)
It, keeping bacterium germination temperature is 19 DEG C.
(5) illumination: will through bacterium germination treated media transfer to temperature be 25 DEG C in step (4), intensity of illumination is
It is cultivated 45 days under the light environment of 800lx, is then heated up to 27 DEG C, continue culture 10 days, until growing cordyceps mycelia.Here
Light application time be 10h/ days, and be intermittent irradiation.
(6) it dries: the culture medium for covering with cordyceps mycelia in step (5) being dried to get Moringa Cordyceps militaris is arrived, and
Moisture content≤9% of Moringa Cordyceps militaris after guaranteeing drying.
The Moringa Cordyceps militaris cultivated by the above method, through detecting, cordycepin content reaches 12mg/g, and potassium content reaches
1.5mg/g。
Bacteriostasis spray is prepared by the Moringa Cordyceps militaris, which comprises the following steps: Moringa Cordyceps militaris is added
Enter in water crush mashing, hydrolysising protease is then added, 65 DEG C at a temperature of reaction is hydrolyzed, sprayed after fully reacting
Mist is dry, the extract obtained after drying is added in aqueous solution and is stirred evenly to get bacteriostasis spray is arrived.
The bacteriostasis spray being prepared by Moringa Cordyceps militaris has good fungistatic effect, while can effectively promote
Into the healing of wound surface, and pharmacological property does not stimulate mildly.Through detecting, pH value 11.54 can improve blood of human body pH value,
96.82% is reached to the bacteriostasis rate of staphylococcus aureus, 97.32% is reached to the bacteriostasis rate of Escherichia coli, and stingless to skin
Swash property.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar can understand the contents of the present invention and be implemented, and it is not intended to limit the scope of the present invention, it is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the scope of protection of the present invention.
Claims (10)
1. a kind of cultural method of Moringa Cordyceps militaris, it is characterised in that: it includes the following steps:
(1) prepare nutrient solution: the nutrient solution is made by matching as follows: 20 ~ 25g of glucose, 1 ~ 1.5g of triammonium citrate, sulfuric acid
0.8 ~ 1.2g of magnesium, 1 ~ 5g of potassium dihydrogen phosphate, 10 ~ 30mg of vitamin, water 1000ml；
(2) it prepares culture medium: the nutrient solution obtained in butt and the step (1) is mixed in the ratio of 1:1.3 ~ 1.5
And stir evenly, culture medium is made, wherein the butt is by mass percentage, including following component: leaf of Moringa 10%, silkworm chrysalis
80%, quinoa 10%；
(3) it is inoculated with: strain being seeded in the step (2) in the culture medium obtained, and carries out sterilization treatment；
(4) bacterium germination: treated that the culture medium is put into carries out at bacterium germination in sterile darkroom by sterilized in the step (3)
Reason, keeping bacterium germination temperature is 18 ~ 19 DEG C；
(5) illumination: by the illumination for being 20 ~ 30 DEG C through bacterium germination treated the media transfer to temperature in the step (4)
It is cultivated 45 ~ 55 days under environment, until growing cordyceps mycelia；
(6) it dries: the culture medium for covering with the cordyceps mycelia in the step (5) is dried to get described in
Moringa Cordyceps militaris.
2. a kind of cultural method of Moringa Cordyceps militaris according to claim 1, it is characterised in that: in the step (1),
The vitamin includes vitamin B1And vitamin B12, the vitamin B1And vitamin B12Feed intake mass ratio be 1:1.
3. a kind of cultural method of Moringa Cordyceps militaris according to claim 1, it is characterised in that: in the step (2),
The butt and the nutrient solution mixed proportion are 1:1.4.
4. a kind of cultural method of Moringa Cordyceps militaris according to claim 1, it is characterised in that: in the step (3),
The time of sterilization treatment is 12h.
5. a kind of cultural method of Moringa Cordyceps militaris according to claim 1, it is characterised in that: in the step (4),
The time of bacterium germination processing is 15 days.
6. a kind of cultural method of Moringa Cordyceps militaris according to claim 1, it is characterised in that: in the step (5),
By the media transfer into light environment after, first holding light environment temperature be 25 DEG C, and cultivate 45 days, then will
Light environment is warming up to 27 DEG C, continues culture 10 days.
7. a kind of cultural method of Moringa Cordyceps militaris according to claim 1, it is characterised in that: in the step (5),
Control intensity of illumination is 800lx, and light application time is 8 ~ 10h/ days.
8. a kind of cultural method of Moringa Cordyceps militaris according to claim 1, it is characterised in that: in the step (6),
Moisture content≤9% of the Moringa Cordyceps militaris after drying.
9. Moringa Cordyceps militaris made from a kind of cultural method as described in any one of claim 1-8 claim.
10. a kind of bacteriostasis spray prepared using Moringa Cordyceps militaris as claimed in claim 9, it is characterised in that: described antibacterial
Spray comprises the following steps: the Moringa Cordyceps militaris being added to the water crushing mashing, hydrolysising protease is then added, 55
Reaction is hydrolyzed at a temperature of ~ 65 DEG C, is spray-dried after fully reacting, the extract obtained after drying is added water-soluble
In liquid and stir evenly to get the bacteriostasis spray is arrived.
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