CN113854401A - Fermented traditional Chinese medicine feed additive and preparation method thereof - Google Patents
Fermented traditional Chinese medicine feed additive and preparation method thereof Download PDFInfo
- Publication number
- CN113854401A CN113854401A CN202111189664.3A CN202111189664A CN113854401A CN 113854401 A CN113854401 A CN 113854401A CN 202111189664 A CN202111189664 A CN 202111189664A CN 113854401 A CN113854401 A CN 113854401A
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- China
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- traditional chinese
- chinese medicine
- feed additive
- fermented
- medicine feed
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Classifications
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- A61K36/06—Fungi, e.g. yeasts
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Abstract
The invention discloses a fermented traditional Chinese medicine feed additive and a preparation method thereof, belonging to the technical field of feed additives, wherein the fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 5% of soybean meal, 3% of corn flour, 10% of corn germ, 10% of liquorice, 8% of angelica sinensis, 5% of elecampane, 6% of bighead atractylodes rhizome, 8% of Chinese yam, 5% of dried ginger, 3% of poria cocos, 5% of composite microbial inoculum and the balance of water. The preparation method comprises the following steps: respectively crushing the feed and the traditional Chinese medicine, sieving, and then putting the crushed feed and the traditional Chinese medicine into a mixer according to the specific gravity to be uniformly stirred to obtain a material A; fully mixing the complex microbial inoculum and water in a water storage tank to form mixed liquid B, and throwing the mixed liquid B into a mixer to be fully and uniformly mixed with the material A to form a mixed body; then placing the mixture into fermentation equipment for aerobic fermentation to prepare a fermentation material; and hermetically packaging the fermented material, and performing anaerobic fermentation to obtain a powdery finished product. The invention can improve the drug effect of the traditional Chinese medicine; and reduces the use of antibiotics.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a fermented traditional Chinese medicine feed additive and a preparation method thereof.
Background
In the process of breeding livestock and poultry, diseases such as digestive tract and respiratory tract often occur, which seriously affect the growth of livestock and poultry, even cause death of livestock and poultry, and cause great loss to vast farmers. The use of the feed antibiotics greatly improves the production level of livestock and poultry, the growth promoting mechanism and effect of the feed antibiotics become common knowledge, and the scale and intensification of the animal husbandry become possible.
However, in recent years, the breeding industry has used a large amount of antibiotics, which results in antibiotic residues in the livestock and poultry products, like a stealth bomb. Abuse of antibiotics accelerates the emergence of bacterial resistance and renders one bacterium resistant to multiple antibiotics, thus allowing the emergence of superbacteria. A large amount of antibiotic medicines are used for a long time, so that the intestinal microbial system of animals is destroyed, the immunity and the resistance of the animal body are reduced, and the anti-toxicity and the anti-external infection capability are particularly poor, so that the secondary frequency of diseases is caused, and the health of human bodies is threatened finally.
Therefore, in the technical field of feed additives, there is still a need for research and improvement on feed additives which can replace antibiotics and are safe in small quantity, which is also a research focus and focus in the technical field of feed additives at present and is the starting point for the completion of the invention.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is as follows: the fermented traditional Chinese medicine feed additive and the preparation method thereof are provided, and after the traditional Chinese medicine is fermented by microorganisms, on one hand, the efficacy of the traditional Chinese medicine can be improved, the use amount of the traditional Chinese medicine is reduced, and the use range is enlarged; on the other hand, the enzyme produced by the microorganism can help animals digest feed and promote absorption, thereby reducing the use of antibiotics; and the method is convenient, quick, easy to operate and suitable for popularization and application.
In order to solve the technical problems, the invention adopts the following technical scheme:
a fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 5-10% of soybean meal, 3-5% of corn flour, 10-15% of corn germ, 10-15% of liquorice, 8-10% of angelica, 5-10% of elecampane, 6-10% of bighead atractylodes rhizome, 8-12% of Chinese yam, 5-8% of dried ginger, 3-7% of poria cocos, 5-8% of a compound microbial inoculum and the balance of water.
As an improvement, the compound microbial inoculum comprises bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria and yeast.
As a further improvement, the preparation ratio of the bacillus subtilis, the bacillus licheniformis, the toad venom antibacterial peptide bacillus, the lactic acid bacteria and the yeast is 1:1:1: 2:1.
As a further improvement, the complex microbial inoculum also comprises clostridium butyricum.
As a further improvement, the preparation ratio of the bacillus subtilis, the bacillus licheniformis, the toad venom antibacterial peptide bacillus, the lactic acid bacteria, the yeast and the clostridium butyricum is 1:1:1: 2:1: 1.
as an improvement, the milk veteh also comprises the astragalus membranaceus, and the astragalus membranaceus accounts for 4-8% by mass.
As a further improvement, the traditional Chinese medicine also comprises rhizoma atractylodis, wherein the rhizoma atractylodis accounts for 5-7% by mass.
A preparation method of a fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria and saccharomycetes according to the proportion of 1:1:1:2:1 to form a composite microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma and Poria, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger and poria cocos into a mixer according to the specific gravity, and uniformly stirring to obtain a material A;
(4) fully mixing the composite microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) putting the mixture C into aerobic fermentation equipment, keeping the temperature and humidity constant, and carrying out aerobic fermentation for 24-48 hours to obtain a fermented material D;
(7) and hermetically packaging the fermented material D, sending the packaged fermented material D into a constant-temperature fermentation room, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
As a modification, in the steps (6) and (7), the temperature in the aerobic fermentation equipment and the fermentation room is 37 ℃.
As an improvement, in the step (4), the mass percentages of the raw materials are as follows: 5-10% of soybean meal, 3-5% of corn flour, 10-15% of corn germ, 10-15% of liquorice, 8-10% of angelica, 5-10% of elecampane, 6-10% of bighead atractylodes rhizome, 8-12% of Chinese yam, 5-8% of dried ginger, 3-7% of poria cocos, 5-8% of a compound microbial inoculum and the balance of water.
After the technical scheme is adopted, the invention has the beneficial effects that:
1) according to the invention, after the traditional Chinese medicines are subjected to wall breaking and crushing, the dissolution of active ingredients of the medicines is increased, the extraction rate of active ingredients is improved, and the medicine effect is enhanced;
2) the microbial fermentation is carried out at the constant temperature of 37 ℃, aerobic fermentation is carried out firstly, and then anaerobic fermentation is carried out, so that active ingredients in the traditional Chinese medicine can be protected from being damaged to the maximum extent;
3) the invention adopts pure traditional Chinese medicine and probiotics for fermentation, has no other carriers, effectively protects the active ingredients of the traditional Chinese medicine from being damaged, thereby enhancing the curative effect;
the traditional Chinese medicine contains various nutrient components and bioactive substances, such as protein, amino acid, fat, carbohydrate, vitamin and other nutrient substances required by animals, can extract effective components and active substances in the fermented traditional Chinese medicine feed additive to the maximum extent through the degradation action of microorganisms, and particularly can extract the active components which are difficult to extract by a conventional method and can be dissociated through the fermentation action of the microorganisms, so that the active components can be absorbed and utilized by organisms, and the curative effect is enhanced;
the probiotics and the metabolites thereof can adjust the balance of microbial flora in intestinal tracts, improve the digestion and utilization rate of feed, promote digestion and absorption and greatly improve the health of gastrointestinal tracts and respiratory tracts;
4) the prepared powdery traditional Chinese medicine is easier to be absorbed by animals and has quick curative effect;
5) the invention can increase the immunity of animal organism, improve antibody, improve organism function and improve anti-stress capability;
6) the fermented traditional Chinese medicine feed additive prepared by the interaction of the traditional Chinese medicine and the probiotics is used as the feed additive, so that the feed conversion rate is improved, the feed conversion ratio is reduced, and the breeding cost is reduced; and the use of antibiotics is reduced, no drug residue is left, and no drug resistance is generated.
In conclusion, the fermented traditional Chinese medicine feed additive and the preparation method thereof provided by the invention have the advantages that after traditional Chinese medicines are subjected to microbial fermentation, on one hand, the efficacy of the traditional Chinese medicines can be improved, the use amount of the traditional Chinese medicines is reduced, and the use range is enlarged; on the other hand, the enzyme produced by the microorganism can help animals digest feed and promote absorption, thereby reducing the use of antibiotics; and the method is convenient, quick, easy to operate and suitable for popularization and application.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass:
5-10% of soybean meal, 3-5% of corn flour, 10-15% of corn germ, 10-15% of liquorice, 8-10% of angelica, 5-10% of elecampane, 6-10% of bighead atractylodes rhizome, 8-12% of Chinese yam, 5-8% of dried ginger, 3-7% of poria cocos, 5-8% of a compound microbial inoculum and the balance of water.
The glycyrrhizin, glycyrrhetinic acid, liquiritin and the like contained in the liquorice have strong immunoregulation effect on livestock and poultry, and have obvious effects of resisting virus, diminishing inflammation, protecting liver and detoxifying.
The angelica is sweet in taste and warm in nature, has the effects of tonifying qi and blood and relaxing bowel, and can improve the immunity of the organism after being added for a long time.
Mu Xiang can move qi and alleviate pain, invigorate spleen to promote digestion, and is indicated for indigestion and diarrhea and dysentery with tenesmus.
Bighead atractylodes rhizome, rhizoma Atractylodis Macrocephalae, with the effects of strengthening the spleen and replenishing qi, drying dampness and inducing diuresis, is used for treating spleen deficiency and anorexia, dyspepsia, diarrhea and edema.
Yam, rhizoma Dioscoreae, has the effects of tonifying spleen, nourishing stomach, tonifying lung and reinforcing kidney.
Dried ginger, nutrient enriching substance in feed, natural antibiotic, etc.
Poria cocos, and Poria cocos, can promote diuresis, invigorate the intestines, and is used for spleen deficiency, poor appetite, loose stool and diarrhea.
The composite microbial inoculum comprises bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactobacillus and saccharomycetes.
The bacillus subtilis, the bacillus licheniformis and the toad venom antibacterial peptide bacillus are high-temperature resistant, acid-resistant and alkali-resistant, can effectively pass through the stomach, have high enzyme production performance, and can quickly improve the feed utilization rate. Generates a large amount of antibacterial substances, effectively inhibits pathogenic bacteria and improves the immunity of the organism.
The lactobacillus resists gastric acid and bile salt, grows rapidly, colonizes intestinal tracts, forms a biomembrane, prevents pathogeny from invading to secrete lactic acid and acetic acid rapidly, creates an acid environment in the intestinal tracts, effectively inhibits the proliferation of the pathogeny and promotes the digestion and absorption of feed effectively. The metabolite is rich in vitamin B, amino acids, and polysaccharide, and has effects of improving hair color, enhancing immunity, and promoting growth.
The yeast has high temperature resistance, high activation speed and high mycoprotein content. The thallus contains rich nutrient substances such as: minerals, vitamins, amino acids, nucleotides, growth promoting factors, and the like. Producing large amounts of biological enzymes. Is beneficial to decomposing macromolecular feed raw materials and improving the digestion and absorption of animals and the feed conversion rate.
Preferably, the preparation ratio of the bacillus subtilis, the bacillus licheniformis, the toad venom antibacterial peptide bacillus, the lactic acid bacteria and the yeast is 1:1:1: 2:1.
Preferably, the complex microbial inoculum further comprises clostridium butyricum.
The clostridium butyricum can generate B vitamins and vitamin K, and effectively improve the meat quality of livestock and poultry. Repairing damaged intestinal mucosa, and providing a healthy intestinal tract. Effectively inhibiting the growth of the clostridium welchii, maintaining the balance of intestinal flora, generating amylase, protease, glycosidase and cellulase in the intestinal tract, and greatly improving the digestion and utilization rate of the feed. The clostridium butyricum fermentation can also acidify and soften the feed, improve nutrient substances and palatability, seize space ecological niches and nutrient ecological niches in an animal body with absolute advantages, generate various probiotic substances and improve the utilization rate of the feed. Clostridium butyricum can reduce pollution in houses. Keeping the normalization of microbial flora in animal intestinal tract, generating butroxacin, inhibiting harmful bacteria in intestinal tract, improving animal disease resistance and immunity, reducing death and elimination rate, decomposing harmful substances such as ammonia, indole and hydrogen sulfide, improving foul smell of feces, and reducing ammonia and hydrogen sulfide content in feces.
Preferably, the preparation ratio of the bacillus subtilis, the bacillus licheniformis, the toad venom antibacterial peptide bacillus, the lactic acid bacteria, the yeast and the clostridium butyricum is 1:1:1: 2:1: 1.
preferably, the milk veteh also comprises astragalus root, and the mass percent of the astragalus root is 4-8%.
Astragalus root can enhance the immunity of the organism and promote the production, growth and development.
More preferably, the traditional Chinese medicine also comprises rhizoma atractylodis, and the mass percentage of the rhizoma atractylodis is 5-7%.
Cang Zhu, drying dampness and strengthening spleen, dispelling wind and cold, indicated for damp obstruction of middle energizer, abdominal distention and fullness, and diarrhea.
A preparation method of a fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria and saccharomycetes according to the proportion of 1:1:1:2:1 to form a composite microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma and Poria, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger and poria cocos into a mixer according to the specific gravity, and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature constant and humidity constant, preferably, carrying out aerobic fermentation for 24-48 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, preferably, the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 1
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 5% of soybean meal, 3% of corn flour, 10% of corn germ, 10% of liquorice, 8% of angelica sinensis, 5% of elecampane, 6% of bighead atractylodes rhizome, 8% of Chinese yam, 5% of dried ginger, 3% of poria cocos, 5% of composite microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, and yeast.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria and saccharomycetes according to the proportion of 1:1:1:2:1 to form a composite microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma and Poria, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger and poria cocos into a mixer according to the specific gravity, and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature constant and humidity constant, and carrying out aerobic fermentation for 24 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 2
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 10% of soybean meal, 5% of corn flour, 15% of corn germ, 15% of liquorice, 10% of angelica sinensis, 10% of elecampane, 10% of bighead atractylodes rhizome, 12% of Chinese yam, 8% of dried ginger, 7% of poria cocos, 8% of composite microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, and yeast.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria and saccharomycetes according to the proportion of 1:1:1:2:1 to form a composite microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma and Poria, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger and poria cocos into a mixer according to the specific gravity, and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature constant and humidity constant, and carrying out aerobic fermentation for 48 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 3
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 8% of soybean meal, 5% of corn flour, 13% of corn germ, 13% of liquorice, 9% of angelica sinensis, 8% of elecampane, 8% of bighead atractylodes rhizome, 10% of Chinese yam, 7% of dried ginger, 5% of poria cocos, 7% of composite microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, and yeast.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria and saccharomycetes according to the proportion of 1:1:1:2:1 to form a composite microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma and Poria, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger and poria cocos into a mixer according to the specific gravity, and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature and humidity constant, and carrying out aerobic fermentation for 36 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 4
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 7% of soybean meal, 4% of corn flour, 12% of corn germ, 12% of liquorice, 8% of angelica sinensis, 7% of elecampane, 7% of bighead atractylodes rhizome, 9% of Chinese yam, 6% of dried ginger, 4% of poria cocos, 6% of composite microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, and yeast.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria and saccharomycetes according to the proportion of 1:1:1:2:1 to form a composite microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma and Poria, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger and poria cocos into a mixer according to the specific gravity, and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature and humidity constant, and carrying out aerobic fermentation for 28 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 5
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 9% of soybean meal, 5% of corn flour, 14% of corn germ, 14% of liquorice, 10% of angelica sinensis, 9% of elecampane, 9% of bighead atractylodes rhizome, 11% of Chinese yam, 8% of dried ginger, 7% of poria cocos, 5% of astragalus membranaceus, 5% of rhizoma atractylodis, 8% of a compound microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, yeast and clostridium butyricum.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria, saccharomycetes and clostridium butyricum according to the ratio of 1:1:1:2: 1:1, mixing into a complex microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma, Poria, radix astragali and rhizoma Atractylodis, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger, poria cocos, astragalus and rhizoma atractylodis into a mixer according to the proportion and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature constant and humidity constant, and carrying out aerobic fermentation for 45 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 6
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 7% of soybean meal, 4% of corn flour, 13% of corn germ, 13% of liquorice, 8% of angelica sinensis, 8% of elecampane, 8% of bighead atractylodes rhizome, 10% of Chinese yam, 7% of dried ginger, 4% of poria cocos, 8% of astragalus membranaceus, 5% of rhizoma atractylodis, 7% of a compound microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, yeast and clostridium butyricum.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria, saccharomycetes and clostridium butyricum according to the ratio of 1:1:1:2: 1:1, mixing into a complex microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma, Poria, radix astragali and rhizoma Atractylodis, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger, poria cocos, astragalus and rhizoma atractylodis into a mixer according to the proportion and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature constant and humidity constant, and carrying out aerobic fermentation for 30 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 7
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 10% of soybean meal, 5% of corn flour, 10% of corn germ, 10% of liquorice, 9% of angelica sinensis, 8% of elecampane, 7% of bighead atractylodes rhizome, 9% of Chinese yam, 8% of dried ginger, 3% of poria cocos, 7% of astragalus membranaceus, 7% of rhizoma atractylodis, 8% of a compound microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, yeast and clostridium butyricum.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria, saccharomycetes and clostridium butyricum according to the ratio of 1:1:1:2: 1:1, mixing into a complex microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma, Poria, radix astragali and rhizoma Atractylodis, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger, poria cocos, astragalus and rhizoma atractylodis into a mixer according to the proportion and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature constant and humidity constant, and carrying out aerobic fermentation for 33 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 8
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 7% of soybean meal, 5% of corn flour, 10% of corn germ, 11% of liquorice, 8% of angelica sinensis, 10% of elecampane, 8% of bighead atractylodes rhizome, 11% of Chinese yam, 6% of dried ginger, 6% of poria cocos, 8% of astragalus membranaceus, 6% of rhizoma atractylodis, 7% of a compound microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, yeast and clostridium butyricum.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria, saccharomycetes and clostridium butyricum according to the ratio of 1:1:1:2: 1:1, mixing into a complex microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma, Poria, radix astragali and rhizoma Atractylodis, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger, poria cocos, astragalus and rhizoma atractylodis into a mixer according to the proportion and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature constant and humidity constant, and carrying out aerobic fermentation for 42 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
Example 9
A fermented traditional Chinese medicine feed additive comprises the following raw materials in percentage by mass: 9% of soybean meal, 4% of corn flour, 12% of corn germ, 13% of liquorice, 9% of angelica sinensis, 7% of elecampane, 6% of bighead atractylodes rhizome, 12% of Chinese yam, 5% of dried ginger, 6% of poria cocos, 6% of astragalus membranaceus, 6% of rhizoma atractylodis, 6% of a compound microbial inoculum and the balance of water.
In this embodiment, the complex microbial inoculum includes bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria, yeast and clostridium butyricum.
The preparation method of the fermented traditional Chinese medicine feed additive comprises the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria, saccharomycetes and clostridium butyricum according to the ratio of 1:1:1:2: 1:1, mixing into a complex microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma, Poria, radix astragali and rhizoma Atractylodis, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger, poria cocos, astragalus and rhizoma atractylodis into a mixer according to the proportion and uniformly stirring to obtain a material A;
(4) fully mixing the complex microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) placing the mixture C into aerobic fermentation equipment, keeping the temperature and humidity constant, and carrying out aerobic fermentation for 38 hours in the aerobic fermentation equipment at the temperature of 37 ℃ to obtain a fermentation material D;
(7) and hermetically packaging the fermented material D, and sending the packaged fermented material D into a constant-temperature fermentation room, wherein the temperature of the fermentation room is 37 ℃, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
The following example 1 is used to illustrate the application effect of the fermented traditional Chinese medicine feed additive on laying hens.
1 materials and methods
1.1 design of the experiment
270 healthy 42-week-old Jinghong laying hens are selected for the test and divided into 3 groups, namely a control group, a test 1 group and a test 2 group, wherein each group is repeated for 3 times, and each group is repeated for 30 times. The control group is fed with basic ration, and the test group is fed with fermented traditional Chinese medicine feed additives accounting for 0.5 percent and 1 percent of the total weight of the basic ration respectively.
1.2 test daily ration
The experimental ration was a corn-soybean meal type basal ration, and the formula of the ration is shown in table 1.
TABLE 1 basic diet formula
Raw materials | Content/% |
Corn (corn) | 59.4 |
Bean pulp | 28 |
Wheat bran | 3 |
Stone powder | 8 |
Calcium hydrogen phosphate | 1.3 |
Salt (salt) | 0.3 |
Total up to | 100 |
1.3 Breeding management
Test chickens fed with free food and water 2 times a day (9 am and 16 pm); the temperature, humidity and illumination of the henhouse are carried out according to the conventional feeding management, the disinfection and epidemic prevention are carried out according to the conventional procedures, the pre-feeding period is 7 days, and the test period is 70 days.
1.4 sample Collection
Eggs are collected at the right time at 17:00 every day, the egg number and the egg weight are recorded every day by taking the repeated groups as units, the feed consumption is counted every week, and the average laying rate, the average egg weight, the feed-egg ratio and the shell breaking rate in the whole period are calculated.
2 influence of fermented traditional Chinese medicine feed additive on laying hen production performance
TABLE 2 influence of fermented traditional Chinese medicine feed additives on the production performance of laying hens
Parameter(s) | Control group | Test 1 group | Test 2 groups |
Egg production rate/%) | 90.54 | 91.83 | 93.98 |
Average egg weight/g | 59.41 | 60.26 | 61.93 |
Material to egg ratio | 2.18 | 2.15 | 2.01 |
Rate of crust breaking | 0.82 | 0.59 | 0.44 |
As can be seen from Table 2, compared with the control group, the laying rate of each test group is higher than that of the control group, the laying rate of the test group 2 is up to 93.98% at most, and the laying rate is improved by 3.44% compared with that of the control group; compared with a control group, the weight average of eggs of each test group is higher than that of the control group, the weight of the eggs of the test 2 group is 61.93g, and the weight of the eggs is increased by 2.52g compared with that of the control group; compared with the control group, the feed-egg ratio and the shell breaking rate of each test group are lower than those of the control group.
The following will illustrate the effect of the fermented Chinese medicinal feed additive on weight gain and disease resistance of fattening pigs in example 9.
1 materials and methods
1.1 time and place of experiment
The test was completed in a pig farm in Henan, 11 months in 2020.
1.2 selection and grouping of test animals
38 ordinary fattening pigs with the same source, the same age in days, similar body weight and good health condition are selected and randomly divided into 4 treatment groups, 8 control groups, 10 test groups 1, 9 test groups 2 and 11 test groups 3.
1.3 test period
The test period was 30 days.
1.4 test design and test daily ration
The experimental ration was a corn-soybean meal type basal ration, and the formula of the ration is shown in table 3. Adding antibiotics into the basic daily ration to feed test pigs of a control group; the experimental group was fed with a ration supplemented with fermented traditional Chinese medicine feed additives, i.e., fermented traditional Chinese medicine feed additives in an amount of 0.5%, 1% and 1.5% of the total weight of the basal ration were added on the basis of the basal ration.
TABLE 3 basic daily ration
Raw materials | Proportioning |
Corn (corn) | 54.3 |
Wheat bran | 20 |
Bean pulp | 12 |
Rice and rice | 9 |
Fish meal | 2.46 |
Calcium hydrogen phosphate | 1.6 |
Stone powder | 0.4 |
Salt (salt) | 0.24 |
Total up to | 100.00 |
1.5 daily management
Semi-open pigsty, dry powdered feed, free for food intake and water drinking; conventional procedures for immune disinfection.
1.6 measurement index and method
1.6.1 feed intake (g/head/day): accurately recording the feeding amount of each group every day, cleaning the trough in the morning next day, and calculating the average daily food intake.
1.6.2 daily gain (g/head/day): early morning on the day of start of the test and early morning on the next day of end of the test, the weights were weighed on empty stomach, and the total weight gain and average daily weight gain were calculated from the initial weight and final weight.
1.6.3 feed-meat ratio: the feed conversion ratio of each group is calculated according to the feed intake and daily gain.
1.6.4 diarrhea rate (%): diarrhea was observed at least 3 times daily at any time, and the average daily diarrhea rate was calculated by recording the number of diarrhea pigs at the end of the trial.
1.6.5 lymphocyte transformation assay: and (3) when the test period is over half, collecting blood by 4 blood collection of each treatment group before feeding in the morning, collecting blood by adopting a sterile anterior vena cava, and respectively injecting into a vacuum EDTA anticoagulation tube and a vacuum biochemical tube to prepare anticoagulation blood and serum. The MTT method was used to determine the peripheral blood lymphocyte transformation rate.
2 results and analysis
2.1 Effect on weight gain Performance and diarrhea Rate in fattening pigs
TABLE 4 influence on weight gain Performance and diarrhea Rate in fattening pigs
Treatment of | Average daily food intake | Average daily gain | Meat ratio of materials | Diarrhea Rate (%) |
Control group | 2225.8 | 718 | 3.1 | 9 |
Test group 1 | 2537.5 | 1015 | 2.5 | 4 |
Test group 2 | 2241 | 830 | 2.7 | 5 |
Test group 3 | 2044 | 730 | 2.8 | 6 |
As can be seen from Table 4, compared with the control group, the average daily food consumption of the test 1 group and the test 2 group is higher than that of the control group, and the average daily food consumption of the test 1 group is 2537.5g, which indicates that the fermented traditional Chinese medicine feed additive can improve the food consumption of the fattening pigs to a certain extent; compared with a control group, the average daily gain weight of each test group is higher than that of the control group, and the average daily gain of the test 1 group reaches 1015 g; compared with a control group, the feed-meat ratio of each test group is lower than that of the control group, and the feed-meat ratio of the test 1 group reaches 2.5; compared with the control group, the diarrhea rate of each test group is lower than that of the control group. Therefore, the fermented traditional Chinese medicine feed additive provided by the embodiment of the invention can improve the weight-gaining performance of fattening pigs and reduce the diarrhea rate.
2.2 Effect on disease resistance of fattening pigs
TABLE 5 Effect on disease resistance in fattening pigs
As can be seen from table 5, the test group had higher peripheral blood T lymphocyte conversion rate and peripheral blood B lymphocyte conversion rate than the control group. Therefore, the fermented traditional Chinese medicine feed additive provided by the embodiment of the invention can improve the disease resistance of the fattening pigs.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (10)
1. The fermented traditional Chinese medicine feed additive is characterized by comprising the following raw materials in percentage by mass: 5-10% of soybean meal, 3-5% of corn flour, 10-15% of corn germ, 10-15% of liquorice, 8-10% of angelica, 5-10% of elecampane, 6-10% of bighead atractylodes rhizome, 8-12% of Chinese yam, 5-8% of dried ginger, 3-7% of poria cocos, 5-8% of a compound microbial inoculum and the balance of water.
2. The fermented traditional Chinese medicine feed additive according to claim 1, wherein the complex microbial inoculum comprises bacillus subtilis, bacillus licheniformis, bacillus bufonis antimicrobial peptide, lactic acid bacteria and yeast.
3. The fermented traditional Chinese medicine feed additive according to claim 2, wherein the ratio of the bacillus subtilis, the bacillus licheniformis, the bacillus bufonis antimicrobial peptide, the lactic acid bacteria and the yeast is 1:1:1: 2:1.
4. The fermented traditional Chinese medicine feed additive according to claim 2, wherein the complex microbial inoculum further comprises clostridium butyricum.
5. The fermented traditional Chinese medicine feed additive according to claim 4, wherein the ratio of the bacillus subtilis, the bacillus licheniformis, the bacillus bufonis antimicrobial peptide, the lactic acid bacteria, the yeast and the clostridium butyricum is 1:1:1: 2:1: 1.
6. the fermented traditional Chinese medicine feed additive according to claim 1, further comprising astragalus membranaceus, wherein the astragalus membranaceus accounts for 4-8% by mass.
7. The fermented traditional Chinese medicine feed additive according to claim 6, further comprising rhizoma atractylodis, wherein the rhizoma atractylodis accounts for 5-7% by mass.
8. A preparation method of a fermented traditional Chinese medicine feed additive is characterized by comprising the following steps:
(1) mixing bacillus subtilis, bacillus licheniformis, toad venom antibacterial peptide bacillus, lactic acid bacteria and saccharomycetes according to the proportion of 1:1:1:2:1 to form a composite microbial inoculum;
(2) respectively pulverizing soybean meal, corn flour, corn germ, Glycyrrhrizae radix, radix Angelicae sinensis, radix aucklandiae, Atractylodis rhizoma, rhizoma Dioscoreae, Zingiberis rhizoma and Poria, and sieving with 100 mesh sieve;
(3) putting the crushed bean pulp, corn flour, corn germ, liquorice, angelica, costus root, bighead atractylodes rhizome, Chinese yam, dried ginger and poria cocos into a mixer according to the specific gravity, and uniformly stirring to obtain a material A;
(4) fully mixing the composite microbial inoculum and water in a water storage tank to form a mixed solution B;
(5) pumping the mixed solution B into a mixer through a water pump, and fully and uniformly stirring the mixed solution B and the material A to form a mixed solution C;
(6) putting the mixture C into aerobic fermentation equipment, keeping the temperature and humidity constant, and carrying out aerobic fermentation for 24-48 hours to obtain a fermented material D;
(7) and hermetically packaging the fermented material D, sending the packaged fermented material D into a constant-temperature fermentation room, and performing anaerobic fermentation for 7 days to obtain a powdery finished product.
9. The method for preparing fermented traditional Chinese medicine feed additive according to claim 8, wherein the temperature in the aerobic fermentation equipment and the fermentation room is 37 ℃ in the steps (6) and (7).
10. The preparation method of the fermented traditional Chinese medicine feed additive according to claim 8, wherein in the step (4), the raw materials are as follows by mass percent: 5-10% of soybean meal, 3-5% of corn flour, 10-15% of corn germ, 10-15% of liquorice, 8-10% of angelica, 5-10% of elecampane, 6-10% of bighead atractylodes rhizome, 8-12% of Chinese yam, 5-8% of dried ginger, 3-7% of poria cocos, 5-8% of a compound microbial inoculum and the balance of water.
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Application publication date: 20211231 |