CN115316538A - Fermented preparation and feed suitable for piglets - Google Patents
Fermented preparation and feed suitable for piglets Download PDFInfo
- Publication number
- CN115316538A CN115316538A CN202211065251.9A CN202211065251A CN115316538A CN 115316538 A CN115316538 A CN 115316538A CN 202211065251 A CN202211065251 A CN 202211065251A CN 115316538 A CN115316538 A CN 115316538A
- Authority
- CN
- China
- Prior art keywords
- weight
- feed
- parts
- fermentation
- lactobacillus casei
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 238000000855 fermentation Methods 0.000 claims abstract description 34
- 230000004151 fermentation Effects 0.000 claims abstract description 34
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 17
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 17
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 10
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 9
- 150000001875 compounds Chemical class 0.000 claims abstract description 6
- 240000008042 Zea mays Species 0.000 claims description 14
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 14
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 14
- 235000005822 corn Nutrition 0.000 claims description 14
- 235000019764 Soybean Meal Nutrition 0.000 claims description 13
- 239000004455 soybean meal Substances 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 claims description 6
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 235000005911 diet Nutrition 0.000 claims description 5
- 230000037213 diet Effects 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 claims description 3
- 229960002685 biotin Drugs 0.000 claims description 3
- 235000020958 biotin Nutrition 0.000 claims description 3
- 239000011616 biotin Substances 0.000 claims description 3
- 229940000252 folic acid 2 mg Drugs 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 3
- 229960003512 nicotinic acid Drugs 0.000 claims description 3
- 235000001968 nicotinic acid Nutrition 0.000 claims description 3
- 239000011664 nicotinic acid Substances 0.000 claims description 3
- 229940055726 pantothenic acid Drugs 0.000 claims description 3
- 235000019161 pantothenic acid Nutrition 0.000 claims description 3
- 239000011713 pantothenic acid Substances 0.000 claims description 3
- 235000019733 Fish meal Nutrition 0.000 claims description 2
- 239000004467 fishmeal Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 235000012424 soybean oil Nutrition 0.000 claims description 2
- 239000003549 soybean oil Substances 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 30
- 238000012360 testing method Methods 0.000 description 16
- 229910021529 ammonia Inorganic materials 0.000 description 15
- 230000013872 defecation Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 241000282887 Suidae Species 0.000 description 7
- 230000004584 weight gain Effects 0.000 description 7
- 235000019786 weight gain Nutrition 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 238000007792 addition Methods 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 239000002131 composite material Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 4
- 235000021050 feed intake Nutrition 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 244000144977 poultry Species 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 235000021195 test diet Nutrition 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 235000020940 control diet Nutrition 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 241000193749 Bacillus coagulans Species 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
- 241001112696 Clostridia Species 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 241000191998 Pediococcus acidilactici Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 229940054340 bacillus coagulans Drugs 0.000 description 1
- 235000014590 basal diet Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000006180 nutrition needs Nutrition 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Birds (AREA)
- Marine Sciences & Fisheries (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to the technical field of biological fermentation, and discloses a fermentation preparation suitable for piglets, which is prepared by fermenting feed with compound bacteria; the compound bacteria are the combination of lactobacillus casei, clostridium butyricum and bacillus licheniformis; the concentration ratio of the lactobacillus casei, the clostridium butyricum and the bacillus licheniformis is as follows: 1:8-12:15-25. The fermented preparation can reduce odor emission of piglets after being added into feed.
Description
Technical Field
The invention relates to the technical field of biological fermentation, in particular to a fermentation preparation and a feed suitable for piglets.
Background
The adoption of strains, particularly composite strains, to reduce the ammonia nitrogen and odor emission of animals is the direction of current research of many technical personnel, such as:
d1: CN202010502972.6 discloses a preparation containing a mixed bacteria fermentation liquid, wherein the mixed bacteria fermentation liquid is obtained by inoculating and fermenting pediococcus acidilactici, lactobacillus acidophilus and bacillus coagulans.
The applicant proposes a patent application in advance, and the main idea is to improve the meat-to-feed ratio by fermenting the daily feed with composite bacteria, and the method specifically comprises the following steps: d2: CN201811277570.X discloses a fermented feed, which comprises an aqueous solution of composite zymophyte and a feed to be fermented, wherein the feed to be fermented is mainly prepared from the following raw materials: soybean meal, corn, bran and rice bran meal; the composite zymocyte consists of lactobacillus casei LC3 and bacillus subtilis. The preparation method of the fermented feed comprises the following steps: mixing soybean meal, corn, bran and rice bran meal uniformly, adding the composite zymocyte aqueous solution, subpackaging after mixing uniformly, tightening the bag opening after air is removed, and fermenting for 2-4 days at 20-37 ℃. The fermented feed can obviously increase the content of lactic acid, simultaneously reduce the generation of acetic acid with pungent smell, has good palatability and low addition amount, can improve the feed intake and weight gain speed of piglets, promote the enzyme activity of serum antioxidant enzyme of the piglets and the secretion of growth axis hormone, reduce bacterial endotoxin of excrement and blood of the preserved pigs, improve the meat color and the tenderness of the fattening pigs and reduce the drip loss after being added into the feed.
Air pollution caused by harmful gases emitted in agricultural production has also become an environmental problem of great concern. According to statistics of environmental protection departments in Shanghai cities, the proportion of odor disturbing complaints of farms in the prior art accounts for more than 70%. Although the livestock and poultry breeding industry pollutant emission standard (GB 18596-2001) requires that the odor concentration of a livestock and poultry breeding farm is less than or equal to 70 (dimensionless), relevant departments such as environmental protection and the like pay special attention to the odor disturbing people of the livestock and poultry breeding farm in recent years. The scale pig farm faces great pressure on odor emission and the like, and the emission reduction of the odor in the pig farm has remarkable ecological environmental benefits, can also be beneficial to improving the living environment around the farm, and has obvious social benefits. In 2018, the Shanghai city issues 'discharge Standard of pollutants for livestock and poultry Breeding' (DB 31/1098-2018) and requires that the concentration of odor in a pig farm in 2020 is less than or equal to 20 (dimensionless). Emission reduction of odor is one of the main problems faced by the current large-scale pig farms.
When trying to reduce ammonia nitrogen and odor emission by adopting a scheme of D2, the method is found to have certain effect, so that the research aim of the application is to further optimize on the basis of the D2 and propose a new preparation for reducing the odor emission without reducing the growth performance of animals.
Disclosure of Invention
The invention aims to provide a fermentation preparation and a feed suitable for piglets, wherein the fermentation preparation can reduce the odor emission of the piglets after being added into the feed, and has similar effects on fattening pigs or pigs in other growth stages.
In order to achieve the purpose, the invention provides the following technical scheme: a fermentation preparation is prepared by fermenting feed with compound bacteria to obtain fermentation preparation; the compound bacteria is the combination of lactobacillus casei, clostridium butyricum and bacillus licheniformis; the concentration ratio of the lactobacillus casei to the clostridium butyricum to the bacillus licheniformis is as follows: 1:8-12:15-25.
In the above fermentation preparation, the feed is a combination of soybean meal, corn flour and bran; the weight ratio of the soybean meal to the corn flour to the bran is 4-6:2-4:2.
in the above fermentation preparation, the preparation method of the fermentation preparation comprises: adding 2% of water of bacterium liquid into the feed, uniformly mixing the feed and the water according to the weight-volume ratio of 1.8-2.2;
the concentration of Lactobacillus casei in the bacterial liquid is (0.8-1.2) x 10 9 cfu/kg。
In the fermentation preparation, the feed and the water are proportioned according to the weight-volume ratio of 2;
the concentration ratio of lactobacillus casei, clostridium butyricum and bacillus licheniformis is 1:10:20;
the concentration of Lactobacillus casei in the bacterial liquid is 1 × 10 9 cfu/kg。
Meanwhile, the invention also provides a feed which contains 4-6 parts by weight of the fermentation preparation, 63-64 parts by weight of corn, 20-21 parts by weight of soybean meal and 2.5-3.5 parts by weight of bran.
The feed comprises 5 parts by weight of fermentation preparation, 63.5 parts by weight of corn, 20.5 parts by weight of soybean meal, 3 parts by weight of bran, 3 parts by weight of fish meal, 1 part by weight of soybean oil and 4 parts by weight of premix;
the premix is provided for each kilogram of feed: cu 60mg, zn 60mg, fe 150.3mg, mn 85.9mg, se 0.3mg, I0.14mg, VA 2000IU, VD 3 1500IU,VE 53mg,VK 3 1mg,VB 1 6mg,VB 2 2.8mg,VB 6 2.8mg,VB 12 0.1mg, folic acid 2mg, nicotinic acid 8mg, pantothenic acid 28mg, biotin 0.2mg.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the biological fermentation material is added into the feed, so that the weight gain of piglets is improved, the feed intake is reduced, and the feed conversion efficiency is improved; the odor intensity detection results of different areas in the pigsty show that the odor intensity of a defecation area in the pigsty is reduced by the biological fermented feed (15.56%, P = 0.076). The addition of the biological fermentation material in the feed can reduce the odor intensity value of a feeding area (5.36%) and a lying area (3.58%) but does not reach a remarkable level. The detection results of ammonia concentration in air in different areas in the pigsty show that the ammonia concentration in the defecation area in the pigsty is reduced by 17.99% (P = 0.046) by the biological fermented feed, but the ammonia concentration in the feeding area and the lying area is not obviously influenced.
Detailed Description
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The sources of all strains of the invention are as follows:
lactobacillus casei: the lactobacillus casei LC3 in CN201811277570.X is stored in the laboratory;
clostridium butyricum: provided by Gyama, kagayi Nutrition medicine (Shandong) micro-ecological research institute, inc., of China, with a specification of 100 hundred million CFU/g;
b, bacillus licheniformis: supplied by Vekayas bioengineering, inc., having a specification of 200 hundred million CFU/g.
Example 1
Preparation of fermentation preparation
The fermentation raw materials are bean pulp, corn flour and bran according to the ratio of 5:3:2, adding 2 percent of bacteria solution water according to the solid-liquid ratio of 2/1 (weight-volume ratio), uniformly mixing, filling into a fermentation bag equipped with a one-way vent valve, sealing and storing at room temperature. The fermentation raw material after 7 days of room temperature fermentation is used for preparing complete feed for piglets.
The concentration of each bacterium in the bacterium liquid is as follows:
1×10 9 cfu/kg of Lactobacillus casei, 1X 10 10 Clostridia butyrate (cfu/kg), 2X 10 10 cfu/kg of Bacillus licheniformis.
Comparative example 1
Reference to description of example 2 of D2:
preparation of fermented feed
Mixing 250 kg of soybean meal, 150 kg of corn flour and 100 kg of bran, then adding 5 liters of bacterial liquid (the weight ratio of lactobacillus casei LC3 to bacillus subtilis is 1.
Example 2
Piglet breeding experiment
2.1 test animals and groups
180 healthy 60-day-old DLY weaned piglets (14.39 +/-3.28 kg) are selected in the test, randomly divided into 3 groups according to the weight consistency principle, and fed to a basic diet group, a test diet group (example 1) and a comparison diet group (comparative example 1) respectively, wherein each group has 6 repetitions, each repetition has 10 pigs, and the formal test period is 4 weeks.
Three groups of piglets are all raised in the same pigsty, three groups are evenly distributed at different positions in the pigsty, and the pigsty is divided into cement solid walls to reduce the mutual interference of gases in the small environments of the adjacent pigsties.
The conditions of all groups are completely the same except for different daily rations, the animal feeding management is carried out according to a conventional mode, the animals can freely take food and drink water, and the animals can be normally inoculated with the vaccine according to the immunization program of a pig farm in the test period.
2.2 basic diet
The basal diets were formulated with reference to the nutritional needs of NRC pigs in the united states (2012), and the composition and nutritional levels of the diets are shown in table 1.
The test ration was supplemented with 5% biofermentation feed (dry weight basis, example 1) while reducing 2.5% soybean meal, 1.5% corn and 1% bran in the base formula so that the base ration and the base materials in the test group ration formula were in the same proportions and additions.
The addition of 5% biofermentation feed (dry weight basis, comparative example 1) was added to the control ration, while reducing 2.5% soybean meal, 1.5% corn and 1% bran in the base formula, so that the base ration was the same as the base material and addition in the test group ration formula.
TABLE 1 test daily formula and nutritional level
Note: 1 the premix in the basic ration is provided for each kilogram of feed: cu 60mg, zn 60mg, fe 150.3mg, mn 85.9mg, se 0.3mg, I0.14mg, VA 2000IU and VD 3 1500IU,VE 53mg,VK 3 1mg,VB 1 6mg,VB 2 2.8mg,VB 6 2.8mg,VB 12 0.1mg, folic acid 2mg, nicotinic acid 8mg, pantothenic acid 28mg, biotin 0.2mg.
2.3 detection index and method
2.3.1 Performance
The test pigs were weighed in columns every two weeks, the feed consumption was determined and ADG, ADFI and feed-meat ratio (F/G) were calculated.
2.3.2 measurement of odor intensity and Ammonia concentration
On the 14 th test day, the odor intensity of the feeding area, the rest area and the defecation area of different swineries is measured by a PEN3.5 portable electronic nose odor analyzer, and the ammonia concentration of different areas of the swineries is measured by an air sampler and a disposable ammonia detection tube. The columns were washed with water 3 pm before the measurement, and the measurement was carried out in a fixed order at 7 am on the day of the measurement, and the measurement was continued for 3 days at the same time.
2.4 analysis of results
2.4.1 Productivity
As shown in Table 2, the piglet production performance in the biofermentation test group was the best. Compared with the basic ration group, the piglet weight gain of the test ration group and the piglet weight gain of the comparison ration group are obviously improved in 1-2 weeks, and the feed intake is obviously reduced (P is less than 0.05), but the piglet weight gain and the feed intake of the three groups in 3-4 weeks and the whole period of the test have no obvious difference (P is less than 0.05). The feed-meat ratio of piglets of the experimental daily groups and the comparative daily group at 1-2 weeks, 3-4 weeks and the whole period of the experiment is obviously lower than that of the basic daily group (P < 0.05), and the production performance difference of the experimental daily group and the comparative daily group is not obvious (P > 0.05). Therefore, the biological fermentation feed is added into the feed, so that the weight gain of piglets is improved, the feed consumption is reduced, and the feed conversion efficiency of each stage of the experiment is obviously improved.
TABLE 2 influence of biofermented feed on piglet production performance
Note: the experimental data are presented as mean ± standard error, with values in the same row with different lower case letters indicating a significant level of difference (P < 0.05).
2.4.2 odor intensity and Ammonia concentration
Results of odor intensity detection in different regions of the pigsty (table 3) show that the odor intensity of the defecation region in the pigsty of the test daily ration group and the control daily ration group is respectively reduced by 15.56% (P = 0.021) and 7.41% (P = 0.047) compared with that of the basic daily ration group, and the odor intensity of the defecation region of the test daily ration group is reduced by 8.86% (P = 0.043) compared with that of the control daily ration group. In addition, the biological fermentation material added into the feed tends to reduce the odor intensity of a feeding area and a lying area, but the difference does not reach a significant level (P > 0.05).
TABLE 3 influence of biofermented feed on odor intensity in different areas of the pigsty
Note: experimental data are presented as mean ± standard error, with values in the same row with different lower case letters indicating a significant level of difference (P < 0.05).
The results of the ammonia concentration measurements in the air in different areas of the pigsty (table 4) show that the ammonia concentration in the defecation area of the pigsty was reduced by 17.99% (P = 0.046) and 8.75% (P = 0.078) in the test and control diets, and the ammonia concentration in the defecation area of the test diet was reduced by 10.13% (P = 0.066) compared to the control diet. The biological fermentation material added into the feed has no obvious influence on the ammonia concentration in a feeding area and a lying area (P is more than 0.05).
TABLE 4 influence of biologically fermented feed on air ammonia concentration in different areas of the swinery
2.5 summary knot
1. The addition of 5% of biological fermentation material in the feed obviously increases the weight gain of piglets, reduces the feed consumption and improves the feed conversion efficiency.
2. The 5% biological fermentation material added in the feed obviously reduces the odor intensity and ammonia concentration of a defecation area in the pigsty, but has no influence on the odor intensity and ammonia concentration of a feeding area and a lying area.
3. The feed containing 5% of the fermented biological material shown in example 1 was more effective in reducing the strength of the odor in the pigsty and the ammonia concentration than the fermented biological material shown in comparative example 1.
Claims (6)
1. A fermentation preparation suitable for piglets is characterized in that a compound bacterium is adopted to ferment feed to obtain a fermentation preparation; the compound bacteria are the combination of lactobacillus casei, clostridium butyricum and bacillus licheniformis; the concentration ratio of the lactobacillus casei to the clostridium butyricum to the bacillus licheniformis is as follows: 1:8-12:15-25.
2. The fermentation formulation of claim 1, wherein the feed is a combination of soybean meal, corn meal, and bran; the weight ratio of the soybean meal, the corn flour and the bran is 4-6:2-4:2.
3. the fermentation preparation according to claim 1, wherein the fermentation preparation is prepared by the following steps: adding 2% of water of bacterium liquid into the feed, uniformly mixing the feed and the water according to the weight-volume ratio of 1.8-2.2;
the concentration of Lactobacillus casei in the bacterial liquid is (0.8-1.2) x 10 9 cfu/kg。
4. The fermentation preparation according to claim 3, wherein the feed and the water are proportioned in a weight-to-volume ratio of 2;
the concentration ratio of lactobacillus casei, clostridium butyricum and bacillus licheniformis is 1:10:20;
the concentration of Lactobacillus casei in the bacterial liquid is 1 × 10 9 cfu/kg。
5. A feed characterized by comprising 4 to 6 parts by weight of the fermented preparation according to any one of claims 1 to 4, 63 to 64 parts by weight of corn, 20 to 21 parts by weight of soybean meal, and 2.5 to 3.5 parts by weight of bran.
6. The feed of claim 5, comprising 5 parts by weight of the fermented preparation, 63.5 parts by weight of corn, 20.5 parts by weight of soybean meal, 3 parts by weight of bran, 3 parts by weight of fish meal, 1 part by weight of soybean oil, 4 parts by weight of premix;
the premix is provided for each kilogram of diet：Cu 60mg,Zn 60mg,Fe 150.3mg,Mn 85.9mg,Se 0.3mg,I 0.14mg,VA 2000IU,VD 3 1500IU,VE 53mg,VK 3 1mg,VB 1 6mg,VB 2 2.8mg,VB 6 2.8mg,VB 12 0.1mg, folic acid 2mg, nicotinic acid 8mg, pantothenic acid 28mg, biotin 0.2mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211065251.9A CN115316538A (en) | 2022-09-01 | 2022-09-01 | Fermented preparation and feed suitable for piglets |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211065251.9A CN115316538A (en) | 2022-09-01 | 2022-09-01 | Fermented preparation and feed suitable for piglets |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115316538A true CN115316538A (en) | 2022-11-11 |
Family
ID=83930884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211065251.9A Pending CN115316538A (en) | 2022-09-01 | 2022-09-01 | Fermented preparation and feed suitable for piglets |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115316538A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178059A (en) * | 2011-05-13 | 2011-09-14 | 青岛诺和诺康生物技术有限公司 | Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof |
| CN113303404A (en) * | 2021-03-31 | 2021-08-27 | 哈尔滨华晨牧业有限公司 | Fermented piglet feed |
| CN113330107A (en) * | 2019-01-10 | 2021-08-31 | 赢创运营有限公司 | Fermentation liquor and application thereof |
| CN113854401A (en) * | 2021-10-13 | 2021-12-31 | 山东大益生物科技集团有限公司 | Fermented traditional Chinese medicine feed additive and preparation method thereof |
| KR20220048773A (en) * | 2020-10-13 | 2022-04-20 | 경북대학교 산학협력단 | Compositon for Increasing Weight of Pig and Use Thereof |
-
2022
- 2022-09-01 CN CN202211065251.9A patent/CN115316538A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178059A (en) * | 2011-05-13 | 2011-09-14 | 青岛诺和诺康生物技术有限公司 | Microcological feed additive for lowering concentration of ammonia in livestock and poultry house and application thereof |
| CN113330107A (en) * | 2019-01-10 | 2021-08-31 | 赢创运营有限公司 | Fermentation liquor and application thereof |
| KR20220048773A (en) * | 2020-10-13 | 2022-04-20 | 경북대학교 산학협력단 | Compositon for Increasing Weight of Pig and Use Thereof |
| CN113303404A (en) * | 2021-03-31 | 2021-08-27 | 哈尔滨华晨牧业有限公司 | Fermented piglet feed |
| CN113854401A (en) * | 2021-10-13 | 2021-12-31 | 山东大益生物科技集团有限公司 | Fermented traditional Chinese medicine feed additive and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 邓奕妮，等: "乳仔猪功能性生物发酵饲料的营养价值及应用研究", 《饲料研究》, no. 9, pages 60 - 63 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108813289B (en) | Application method and preparation method of preparation for efficiently biodegrading mycotoxin | |
| CN102747065B (en) | High-efficiency fermenting agent for poultry excrement and fermenting method of fermenting agent | |
| CN101449752B (en) | Composite microbial inoculant for corn silage and use method thereo | |
| CN103535323B (en) | The aquaculture model of a kind of livestock and poultry | |
| CN107801837B (en) | Biological fermentation process for forage-based utilization of chicken manure | |
| CN109198214A (en) | A kind of fermented feed, preparation method and its mixed feed | |
| CN102234623A (en) | Microbial preparation and fermentation process thereof | |
| CN100998369A (en) | Bo-gen double micro-organism protein forage | |
| CN110583866A (en) | High-dietary-fiber low-antigen-protein fermented soybean hull and preparation method thereof | |
| CN111728098A (en) | Composite probiotics for natural plant fermentation, feed additive for broiler chickens and preparation method thereof | |
| CN108277188B (en) | Compound microbial starter culture for weaned piglets and application thereof | |
| CN101836688A (en) | Preparation method of antibiotic-free microbial fermentation feed | |
| CN106578354A (en) | Piglet compound premix, preparation method and daily ration for piglet | |
| KR20020093996A (en) | Animal feed additives and method of producing the same | |
| CN104886391A (en) | Biological fermentation feed for reducing odor of pig manure and preparation method of biological fermentation feed | |
| CN109496981B (en) | Method for improving egg laying quality of laying hens | |
| CN110477190A (en) | A kind of stalk anaerobic fermentation feed and preparation process and formula forage | |
| CN115316538A (en) | Fermented preparation and feed suitable for piglets | |
| JPH0734718B2 (en) | Feed additives for farmed fish | |
| CN113854423A (en) | Fermented feed containing various probiotics for live pig breeding | |
| CN110692805A (en) | Composite probiotic fermented feed for animal breeding and preparation method thereof | |
| CN111763638A (en) | Veterinary microecological preparation and preparation method thereof | |
| CN107897505B (en) | Zymophyte liquid, product containing zymophyte liquid and used for nursing pigs, and preparation method and application of product | |
| CN110384183A (en) | A kind of fermentation leached tea oil slag biological feedstuff and preparation method thereof | |
| CN111165680A (en) | Composite probiotic feed additive for geese and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |