Summary of the invention
The objective of the invention is to seek a kind of good palatability, consumption is low, have good disease-resistant growth-promoting effect and have no drug resistance and the pure natural Chinese herbal feed additive of medicament residue to substitute traditional chemicals additive such as antibiotic.
Traditional Chinese medicine research shows, genseng energy tonifying QI to produce body fluid, the intelligence development of calming the nerves; But root of kudzu vine relieving muscles to expel heat, promote the production of body fluid to quench thirst; The grass-leaved sweetflag detoxifcation of drying; Purslane clearing heat and promoting diuresis, removing pattogenic heat from the blood and toxic material from the body; Dried orange peel regulate the flow of vital energy be good for the stomach, eliminating dampness and eliminating phlegm; The rhizoma atractylodis invigorating the spleen to dry, dispel the wind that it is dirty to ward off.In addition, rhizoma atractylodis have good sterilization effect to tubercle bacillus, Escherichia coli, staphylococcus aureus etc.; Dried orange peel can suppress aureus growth; Grass-leaved sweetflag has inhibitory action to dermatophyte.It is collaborative mutually to learn from other's strong points to offset one's weaknesses after compound according to each flavor pharmacological effect of Chinese herbal medicine and Chinese herbal medicine multi-flavor, strengthen the principle of drug effect, the present invention with antipyretic and antidotal type (as purslane), help digestion class (as dried orange peel), tonifying Qi establishing-Yang class (as the folium panacis japonici cum caule) and the extraordinary nutrition class (as hydrolyzed animal protein) of regulating the flow of vital energy wait each big class Chinese herbal medicines reasonable compatibility prescription to make pure natural compound Chinese herb feed addictive.The present invention discovers, compound Chinese herb feed addictive with Chinese herbal medicines such as purslane, the root of kudzu vine, rhizoma atractylodis, dried orange peel, folium panacis japonici cum caule, grass-leaved sweetflag, albumen powder composition, has tangible disease-resistant growth-promoting effect, good palatability, anti-heat stress, safety non-toxic, and less addition can reach ideal effect.
The composition (weight %) of the Chinese herbal feed additive that the present invention proposes is as follows:
Purslane 2.2-2.5
Root of kudzu vine 1.4-1.7
Rhizoma atractylodis 1.2-1.4
Dried orange peel 1.4-1.6
Folium panacis japonici cum caule 1.4-1.6
Grass-leaved sweetflag 1.2-1.4
Hydrolyzed animal protein 0.4-0.5
Mould inhibitor 0.06
Calcium carbonate 89.24-90.74
The reasonable addition of Chinese herbal feed additive of the present invention is the 0.2-0.3% that accounts for feed weight, and under this addition, Chinese herbal feed additive is to weightening finish, the antibacterial effect excellence of animal.
After according to the prescription of said herbal medicine feed addictive each flavor Chinese medicine material being shredded respectively, put respectively in the dish, treat that the ethanol volatilization is placed on 60-80 ℃ of oven for drying, cooling, the pulverizing of 60 orders, takes by weighing supplementary material by formula rate with evenly sterilization of 75% ethanol spraying.To make an appointment with the Paris white of half amount to put into mixer earlier, and slowly add respectively distinguish the flavor of Chinese medicine fine powder and hydrolyzed animal protein while stirring, and then add remaining Paris white and mould inhibitor, finish, mix 20 minutes again to even.Afterwards in shaking screen with 30 order mesh screens once, complete feed is put into the mixer secondary and is stirred 20 minutes to evenly, once more in shaking screen with 30 order mesh screens once, the barrelling weighing, chemically examine packing and make Chinese herbal feed additive of the present invention.
Test the disease-resistant growth-promoting effect that specifies Chinese herbal feed additive of the present invention below by pharmacological testing and feeding of broiler.
1, Chinese herbal feed additive is raised prevention chick Escherichia coli and salmonella and growth promotion test
Adopt 10 age in days reed catkins laying hens from the health of Qingpu one chicken house in this test, basal feed is the full price chicken material that magnificent greenfeed factory produces, and does not contain any chemicals additive.ROX is produced by shake first drugmaker of Zhejiang, and Escherichia coli and salmonella strain are provided by animal doctor's diagnostic center Bacteria Identification chamber, Shanghai City.In the test, divide three groups at random with 60 10 age in days reed catkins chicks, under the identical condition of feeding, the basal feed (attacking bacterium beginning in preceding 12 days) that test group is fed and added 0.2% Chinese herbal feed additive, the ROX group basal feed of feeding, and carry out the 10ppm ROX and drink water (attacking bacterium beginning in preceding 4 days) the control group fed basal feed.Each group is weighed before the test, when being separated by 12 days each winding kind Escherichia coli and salmonella, weighs once more.The inoculation bacteria concentration is that every milliliter of bacteria containing amount is about 10
8CFU, 0.5 milliliter of every plumage chicken abdominal cavity inoculation.
(1) to the gaining effect of chick
Group |
Average every initial body weight of chicken |
Average every final body weight of chicken |
Average every chicken weightening finish |
Test group |
120 |
250 |
130 |
The ROX group |
115 |
215 |
100 |
Control group |
115 |
220 |
105 |
Average every chicken weightening finish of test group is apparently higher than ROX group and control group, and the Chinese herbal feed additive gaining effect of use 0.2% is obvious, and can significantly increase the appetite of chick.
(2) prevention Escherichia coli and salmonella effect
After observing abdominal cavity inoculation Escherichia coli and salmonella, write down the death condition of each group in three days
Group |
The chicken number |
Inoculate three days internal memory live chickens numbers |
Death toll |
The death rate |
Test group |
20 |
14 |
6 |
30% |
The ROX group |
20 |
11 |
9 |
45% |
Control group |
10 |
4 |
6 |
60% |
Find relatively that through conspicuousness the death rate of test group extremely significantly is lower than control group (p<0.01), significantly be lower than ROX drinking-water group (p<0.05)
Above-mentioned two show, add 0.2% Chinese herbal feed additive in chick fodder, have common Escherichia coli of tangible prevention chick and salmonella infection effect, and good growth promoting function is arranged, and effect is better than the antibiotic ROX.
2, Chinese herbal feed additive is prevented and treated the test of chick colibacillosis
Adopt in this test from Shanghai City China Shen great-grandfather for the healthy Luo Man laying hen of an age in days of laying hen field, basal feed is the full price laying hen material that magnificent greenfeed factory produces, and does not contain any chemicals additive.Feeding aureomycin is produced by the big flourish animal health product in Foochow factory, and Escherichia coli standard bacterial classification is provided by Shanghai City animal doctor's diagnostic center, and bacteria containing amount is 10
9-10
10CFU/ml.In the test, divide six groups at random, every group 50 plumage or 30 plumages with an age in days Luo Man chick.Raise chick to 20 order age by daily mode, confirm healthyly through observing, the basal feed and the feeding aureomycin that begin test group and aureomycin control group fed have been added the Chinese herbal feed additive of doses are prevented and treated test.Blank and positive controls are not fed any medicine.After preventing and treating for two weeks, except that the blank group, all the other 5 groups with muscle injection mode inoculation Escherichia coli bacteria liquid 0.5ml/ plumage, observes seven, and each morbidity and death condition of organizing sees the following form:
Group |
Test chicken quantity (plumage) |
Medicine addition (%) |
Attack bacterium sequela number (plumage) |
The incidence of disease (%) |
Death toll (plumage) |
The death rate (%) |
Protective rate (%) |
Test group |
The medicine low dose group |
50 |
0.1 |
35 |
70 |
17 |
34 |
66 |
Dosage group in the medicine |
50 |
0.2 |
31 |
62 |
17 |
34 |
66 |
The medicine high dose group |
50 |
0.3 |
0 |
0 |
0 |
0 |
100 |
Control group |
The aureomycin control group |
50 |
0.002 |
36 |
72 |
18 |
36 |
64 |
Positive controls |
30 |
|
30 |
100 |
22 |
73 |
|
The blank group |
30 |
|
0 |
0 |
0 |
0 |
|
Annotate: the morbidity criterion; Spirit is depressed, feather is in disorder, the ground that crouches, do not drink water, do not eat food; Dead criterion; The morbidity back is dead, cuts open inspection, and Bacteria Culture is the Escherichia coli positive; Protective rate=(test number-death toll)/test number * 100%
Last table shows, Chinese herbal feed additive (0.1%, 0.2%, 0.3% 3 additive capacity) and feeding aureomycin are made an addition under the situation in two weeks of feeding in the chick fodder, the morbidity that prevention artificial infection Escherichia coli are caused has fairly obvious effect with dead, and is a certain amount of effect relationship.Compare with adding feeding aureomycin, the Chinese herb feed additive of interpolation 0.3% extremely significantly is being better than aureomycin aspect the prevention Escherichia coli in the feed, and the effect and the aureomycin effect of interpolation 0.2% and 0.1% are approaching, do not have the statistics difference.Therefore, the 0.3%th, the optimum addn amount of prevention colibacillosis.
3, the Chinese herbal feed additive test that raises table hens
Test adopts " precious star " commodity that fryer field, footpath, hole, Songjiang provides for seedling chicken 150 plumages, and the chick with 150 health during test is divided into 3 groups at random, every group 25 plumage, establish two parallel, added 35ppm aureomycin in the feed of aureomycin control group; Added 0.2% Chinese herbal feed additive in the feed of test group.The feeding experiment cycle was 8 weeks, was divided into raising early stage (0-4 week) and raising later stage (5-8 week).Feeding and management and immune programme for children carry out routinely.Test event comprises weightening finish, the price of deed, survival rate, dressing percentage, meat matter (look, delicate flavour), blood physiology index, medicament residue analysis.
(1) impact effect of the survival rate of chick, weightening finish, the price of deed
Group |
Throw seedling number (plumage) |
Survival rate (%) |
Heavy (gram/plumage) all around |
The end of term heavy (gram plumage) |
Feed consumption (gram/plumage) |
Feedstuff-meat ratio |
Gross profit (unit/plumage) |
The aureomycin control group |
25 |
96 |
569 |
1508±161 |
4103 |
2.72 |
1.14 |
Test group |
25 |
100 |
550 |
1521±137 |
3942 |
2.59 |
1.77 |
The weightening finish of end of term of test group is higher than the aureomycin group, but not significantly (p>0.05), the price of deed of test group and economic benefit (gross profit) are better than the aureomycin group.
(2) butcher, taste measurement result
Group |
Half clean thorax rate (%) |
Complete clean thorax rate (%) |
Color |
Taste |
Tender degree (muscle fibre) |
The aureomycin control group |
86.69 |
83.03 |
3.2 |
3.1 |
4.4 |
Test group |
89.06 |
85.02 |
4.6 |
4.2 |
4.6 |
The dressing percentage result is similar to gaining effect, tastes to measure to show: the color of Chinese herbal medicine test group, taste scoring, all be higher than the aureomycin control group, and tender degree difference is little.Tasting the best result of measuring is 6 minutes.
(3) blood, the residual measurement result of medicine show, each is organized, and the chicken blood index all reaches health standards and difference is not remarkable, and each test group does not all record antibiotic residue.
4, the Chinese herbal feed additive test that raises table hens
The commodity that test employing Shanghai City Great River Stocks Trading Co. Co., Ltd provides are for AA seedling chicken 500 plumages, and the AA chick with 500 health during test is divided into 5 groups at random, and every group 100 plumage do not add any antibiotic and Chinese herbal medicine in the feed of blank group; Added 35ppm aureomycin in the feed of aureomycin control group; Added 0.1%, 0.2%, 0.3% Chinese herbal feed additive in the feed of 1 group in Chinese herbal medicine, 2 groups in Chinese herbal medicine, 3 groups in Chinese herbal medicine respectively.The feeding experiment cycle was 6 weeks, was divided into raising early stage (0-3 week) and raising later stage (4-6 week).Feeding and management and immune programme for children carry out routinely.Test event comprises weightening finish, the price of deed, survival rate, dressing percentage, meat matter (look, delicate flavour)
(1) impact effect of the survival rate of chick, weightening finish, the price of deed
Group |
Chick number (plumage) |
Survival rate (%) |
Three all body weight in age (gram/plumage) |
End of term body weight (gram/plumage) |
Feed consumption (gram/plumage) |
Feedstuff-meat ratio |
The blank group |
100 |
98 |
629±67 |
1653±162 |
3607 |
2.18 |
The aureomycin control group |
100 |
99 |
635±82 |
1685±145 |
3604 |
2.14 |
1 group in Chinese herbal medicine |
100 |
100 |
644±54 |
1698±168 |
3582 |
2.11 |
2 groups in Chinese herbal medicine |
100 |
99 |
722±35 |
1702±149 |
3562 |
2.09 |
3 groups in Chinese herbal medicine |
100 |
99 |
668±70 |
1692±153 |
3575 |
2.11 |
Each forms motility rate does not have difference substantially, and the weightening finish of aureomycin group and the Chinese herbal medicine group end of term, three weightening finishes in ages in week and the price of deed all are significantly higher than control group, and the effect of Chinese herbal medicine group is better than the aureomycin group, in the Chinese herbal medicine group, with the best results of 2 groups in Chinese herbal medicine.
(2) butcher, taste measurement result
Group |
Half clean thorax rate (%) |
Complete clean thorax rate (%) |
Color |
Taste |
Tender degree (muscle fibre) |
The blank group |
87.66 |
84.11 |
3.5 |
3.6 |
3.4 |
The aureomycin control group |
89.36 |
85.45 |
3.1 |
3.4 |
3.4 |
1 group in Chinese herbal medicine |
90.12 |
86.74 |
3.7 |
4.1 |
3.3 |
2 groups in Chinese herbal medicine |
90.86 |
87.25 |
3.8 |
4.2 |
3.4 |
3 groups in Chinese herbal medicine |
90.07 |
86.62 |
3.7 |
3.9 |
3.5 |
The dressing percentage result is similar to gaining effect, tastes to measure to show: the color of each group of Chinese herbal medicine, taste scoring, all be higher than aureomycin group and control group, and tender degree difference is little.Tasting the best result of measuring is 5 minutes.
The result of feeding experiment 3 and feeding experiment 4 shows, sees the growth promotion effect of Chinese herbal medicine group is better than or near the aureomycin group from gaining effect, so the alternative traditional antibiotics growth accelerator of Chinese herbal feed additive of the present invention.On reach two tests and carry out in 7-8 month and 5-6 month respectively, under damp and hot weather, the feed consumption of control group, aureomycin group and efficiency of feed utilization are worse than each group of Chinese herbal medicine, illustrate that Chinese herbal feed additive has the anti-heat stress effect.Chinese herbal feed additive of the present invention has good growth promoting function, and it is residual not produce antibiotic medicine, safe and reliable, and can improve the local flavor of fryer.
From above result of the test, can draw beneficial effect of the present invention and be:
1, to adopt the made additive of Chinese herbal medicines raw material be the Chinese herbal medicine green feed additive of pure natural in the present invention, has safety non-toxic, has no drug resistance and advantage such as antibiotic residue.
2, the present invention's Chinese herbal feed additive of adopting proposition has tangible inhibitory anti-virus and growth promoting function, the feeding antibiotic aureomycin or the ROX that are better than using always in the effect aspect prevent disease and the weightening finish.
3, the Chinese herbal feed additive of the present invention's proposition has the anti-heat stress effect, and good palatability whets the appetite, and can improve the utilization rate of feed, and can improve the local flavor of poultry, improves the quality of poultry.
4, the Chinese herbal feed additive addition of the present invention's proposition is lower, is generally 0.2-0.3%, and of many uses, can be used for chicken, duck, pig, ox, sheep and aquatic livestock.