CN115152893A - Compound probiotic composition for Qingyuan spicy-feather chicks and preparation method thereof - Google Patents
Compound probiotic composition for Qingyuan spicy-feather chicks and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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Abstract
The invention discloses a compound probiotic composition for Qingyuan spicy feather chicks, which is prepared from compound probiotic powder, taro powder, molasses, sodium glutamate and konjac glucomannan; the composite probiotic powder is prepared from the following raw materials in parts by weight: 1-10 parts of bacillus subtilis, 1-5 parts of bacillus licheniformis, 2-7 parts of pediococcus acidilactici, 2-6 parts of lactobacillus, 1-5 parts of bifidobacterium longum and 1-5 parts of vibrio gulcomosus. The invention also discloses a preparation method of the composite probiotic composition for the Qingyuan pockmarked feather chicks, and the composite probiotic composition can increase the daily gain and reduce the feed-weight ratio of the Qingyuan pockmarked chicken, improve the anti-inflammatory factor IL-4 in serum, reduce the level of the proinflammatory factor IFN-gamma, improve the IFN-gamma content in serum relative to antibiotic feeding, and has excellent effect.
Description
Technical Field
The invention belongs to the technical field of probiotic compositions, and particularly relates to a composite probiotic composition for Qingyuan spotted-feather chicks and a preparation method thereof.
Background
China is a big agricultural country, and the animal husbandry plays an important role in agriculture. The animal husbandry in China is rapidly developed, and the data of the national statistical administration shows that the increase value of the agriculture, forestry, animal husbandry and fishery in China reaches 8,103.9 hundred million yuan and the total output value is 137.782.17 hundred million yuan in 2020. Antibiotics have been widely used in livestock production for the past decades to ensure animal health, but lack of complete regulatory regulations for drug use has led to their misuse or misuse. In animal husbandry, the irregular use and abuse of antibiotics can cause drug resistance of bacteria, and simultaneously cause antibiotic residues in animal products, so that superbacteria appear, and the health of human beings can be seriously threatened. Therefore, there is much interest in developing herbal plant additives and antibiotic substitutes such as plant extracts, antimicrobial peptides, probiotics, etc.
The healthy development of poultry farming is often threatened by pathogenic bacteria, especially in chicken breeding. Because the feeding environment is relatively closed and the feeding density is high, dust, excrement and the like exist in the environment, and pathogenic bacteria are easy to breed; in the free-range mode, the breeding environment is open and the conditions are difficult to control, so that the breeding environment is more vulnerable to various pathogenic bacteria. In the past 20 years, antibiotics are added into feed for preventing and controlling diseases of chicken flocks and treating the diseases, so that the health of the chicken is guaranteed, however, with the prohibition of the antibiotics in the poultry industry, the poultry industry faces huge challenges, and effective countermeasures after getting rid of the antibiotics are necessarily considered. The current research shows that the probiotics can obviously promote the absorption of organisms to nutrient substances and enhance the immunity of the organisms, is popularized and applied in animal production, and has the potential of replacing antibiotics. Compared with the traditional basic ration, the probiotic fermented feed for feeding the black-bone chicken can obviously improve the carcass rate and the pectoral muscle rate of the black-bone chicken, obviously improve the content of various amino acids in muscles, improve the feed absorption rate and improve the meat quality. In addition, probiotics are added into the daily ration, so that the immunity of the broiler chicken can be improved, and the adaptability of the broiler chicken to resist heat stress is improved. Researches show that the bacillus is added into basic daily ration, so that the content of bifidobacterium in the caecum of the laying hen can be obviously increased, the content of escherichia coli can be reduced, and the production performance and the egg quality can be effectively improved.
To date, studies on single-strain probiotics or complex probiotics have been mainly conducted on broilers or layers, while relatively few studies have been conducted in the chick stage. In the brooding stage, the immune system of the chick is not completely established, so that the probiotics has important significance for the research on the growth performance, the immune performance, the intestinal flora and the substitution of antibiotics of the chick.
Disclosure of Invention
The invention aims to: aiming at the problems, the composite probiotic composition for the Qingyuan pockmarked-feather chick is provided, which can adjust the intestinal environment of animal organisms, improve the health and production performance of animals, is beneficial to improving the immune function of the Qingyuan pockmarked-feather chick and reducing the IFN-gamma content in the serum of the Qingyuan pockmarked chick, and the preparation method thereof.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
a composite probiotic composition for remote-clearing pockmarked-feather chicks is prepared from composite probiotic powder, taro powder, molasses, sodium glutamate and konjac glucomannan;
the composite probiotic powder is prepared from the following raw materials in parts by weight: 1-10 parts of bacillus subtilis, 1-5 parts of bacillus licheniformis, 2-7 parts of pediococcus acidilactici, 2-6 parts of lactobacillus, 1-5 parts of bifidobacterium longum and 1-5 parts of vibrio gulcomosus.
Preferably, the viable count of each probiotic in the composite probiotic powder is at least 2 x 10 9 cfu/g。
Preferably, the composite probiotic powder consists of the following raw materials in parts by weight: 8 parts of bacillus subtilis, 4 parts of bacillus licheniformis, 3 parts of pediococcus acidilactici, 3 parts of lactobacillus, 2 parts of bifidobacterium longum and 3 parts of vibrio gulcomosus.
Preferably, the composite probiotic composition comprises the following raw materials in parts by weight: 20-45 parts of composite probiotic powder, 30-50 parts of taro powder, 3-5 parts of molasses, 1-5 parts of sodium glutamate and 5-10 parts of konjac glucomannan.
Preferably, the composite probiotic composition comprises the following raw materials in parts by weight: 35 parts of composite probiotic powder, 40 parts of taro powder, 4 parts of molasses, 3 parts of sodium glutamate and 8 parts of konjac glucomannan.
A preparation method of a composite probiotic composition for Qingyuan pockmarked-feather chicks comprises the following steps:
(1) Firstly, stirring and dissolving the taro powder with water at 50-80 ℃;
(2) Cooling to normal temperature, adding molasses, sodium glutamate and konjac glucomannan, and stirring;
(3) And finally, adding the composite probiotic powder into the dissolved solution, and stirring and mixing uniformly.
Preferably, the mass ratio of the taro powder to the water is 1:1-3.
Preferably, the purity of the taro powder is more than 98%.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
(1) The composite probiotic composition can increase the daily gain of Qingyuan pockmarked chicks, reduce the feed-weight ratio, improve the anti-inflammatory factor IL-4 in serum, reduce the level of proinflammatory factor IFN-gamma, improve the content of IFN-gamma in serum compared with antibiotic feeding, and has excellent effect.
(2) The addition of antibiotics in the daily ration not only increases the risk of proinflammatory factors, but also enables an animal body to generate an immunosuppressive state, and the composite probiotic composition provided by the invention has the effects of improving the anti-inflammatory factors in serum, reducing the content of IFN-gamma in the serum, and is researched as an immunopotentiator.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to preferred embodiments. It should be noted, however, that the numerous details set forth in the description are merely for the purpose of providing the reader with a thorough understanding of one or more aspects of the present invention, which may be practiced without these specific details.
Example 1
The composite probiotic composition for the Qingyuan spicy-feather chicks comprises the following raw materials in parts by weight: 35 parts of composite probiotic powder, 40 parts of taro powder and molasses4 parts of sodium glutamate, 3 parts of konjac glucomannan and 8 parts of konjac glucomannan; the composite probiotic powder consists of the following raw materials in parts by weight: 8 parts of bacillus subtilis, 4 parts of bacillus licheniformis, 3 parts of pediococcus acidilactici, 3 parts of lactobacillus, 2 parts of bifidobacterium longum and 3 parts of vibrio gulcomosus, and the viable count of each probiotic in the composite probiotic powder is at least 2 multiplied by 10 9 cfu/g；
A preparation method of a composite probiotic composition for Qingyuan spicy-feather chicks comprises the following steps: firstly, stirring and dissolving taro powder with water of 60 ℃, wherein the mass ratio of taro powder to water is 1:2, the purity of the taro powder is more than 98 percent; cooling to normal temperature, adding molasses, sodium glutamate and konjac glucomannan, and stirring; and finally, adding the composite probiotic powder into the dissolved solution, and stirring and mixing uniformly.
Example 2
Other process parameters of the embodiment are consistent with those of embodiment 1, except that: the composite probiotic composition for the Qingyuan spicy-feather chicks comprises the following raw materials in parts by weight: 20 parts of composite probiotic powder, 30 parts of taro powder, 3 parts of molasses, 1 part of sodium glutamate and 5 parts of konjac glucomannan.
Example 3
Other process parameters of the embodiment are consistent with those of embodiment 1, except that: the composite probiotic composition for the Qingyuan spicy-feather chicks comprises the following raw materials in parts by weight: 45 parts of composite probiotic powder, 50 parts of taro powder, 5 parts of molasses, 5 parts of sodium glutamate and 10 parts of konjac glucomannan.
Example 4
Other process parameters of the embodiment are consistent with those of the embodiment 1, and the difference is that: the composite probiotic powder consists of the following raw materials in parts by weight: 1 part of bacillus subtilis, 1 part of bacillus licheniformis, 2 parts of pediococcus acidilactici, 2 parts of lactobacillus, 1 part of bifidobacterium longum and 1 part of vibrio gulcomycosis, and the viable count of each probiotic in the composite probiotic powder is at least 2 multiplied by 10 9 cfu/g。
Example 5
Other process parameters of the embodiment are consistent with those of embodiment 1, except that: the composite probiotic powder consists of the following componentsThe raw materials in parts by weight are as follows: 10 parts of bacillus subtilis, 5 parts of bacillus licheniformis, 7 parts of pediococcus acidilactici, 6 parts of lactobacillus, 5 parts of bifidobacterium longum and 5 parts of vibrio gulcomycosis, and the viable count of each probiotic in the composite probiotic powder is at least 2 multiplied by 10 9 cfu/g。
Example 6
Other process parameters of the embodiment are consistent with those of the embodiment 1, and the difference is that: a preparation method of a composite probiotic composition for Qingyuan spicy-feather chicks comprises the following steps: firstly, stirring and dissolving the taro powder by using water with the temperature of 50 ℃, wherein the mass ratio of the taro powder to the water is 1:1, the purity of the taro powder is more than 98 percent; cooling to normal temperature, adding molasses, sodium glutamate and konjac glucomannan, and stirring; and finally, adding the composite probiotic powder into the dissolved solution, and stirring and mixing uniformly.
Example 7
Other process parameters of the embodiment are consistent with those of embodiment 1, except that: a preparation method of a composite probiotic composition for Qingyuan spicy-feather chicks comprises the following steps: firstly, stirring and dissolving taro powder in water of 50 ℃, wherein the mass ratio of taro powder to water is 1:3, the purity of the taro powder is more than 98 percent; cooling to normal temperature, adding molasses, sodium glutamate and konjac glucomannan, and stirring; and finally, adding the composite probiotic powder into the dissolved solution, and stirring and mixing uniformly.
The composite probiotic composition prepared in the embodiment 1 of the invention is added into a complete ration, and a feeding test is performed on 1-day-old Qingyuan partridge chickens, and the statistics of the test method and related data are as follows:
1.1 test animals
160 Qingyuan partridge chickens of 1 day old, a composite probiotic composition (containing bacillus subtilis and bacillus licheniformis, the viable count is more than or equal to 2 multiplied by 109 cfu/g), physiological saline, PBS, florfenicol, complete ration and the like are purchased from Nanning Mingheng veterinary service center.
1.2 design of the experiment
160 1-day-old Qingyuan partridge chickens are randomly divided into 4 groups, each group has 4 repetitions, each repetition is 10, and the groups are respectively a control group (basic daily ration), a composite probiotic composition group (basic daily ration + probiotics 750 mg/kg) and an antibiotic group (basic daily ration + florfenicol 200 mg/kg), and are fed freely and drunk freely.
1.3 Effect of each test group on growth performance of Qingyuan partridge chickens
The chickens in each test group were weighed in the whole group 21 and 42 days after feeding (fasting for 12h before weighing, free drinking water), and the Average Daily Gain (ADG), daily feed intake (ADFI) and feed-to-weight ratio (F/G) at different stages after feeding were recorded.
1.4 Effect of each test group on serum immunity index of Qingyuan partridge chickens
On day 42, 4 chickens were randomly drawn for each replicate, and serum was isolated and assayed for IL-4 and IFN- γ content using an enzyme-linked immunosorbent assay (ELISA).
1.5 Effect of each test group on intestinal flora of Qingyuan partridge chickens
On day 42, 4 chickens were randomly selected from each group, cecal contents were aseptically collected, added with 2mL of 1 × PBS solution, stored with dry ice, sent to the shanghai oshi biomedical science and technology limited for 16S rDNA sequencing, and Alpha diversity and cecal microbial composition analysis were performed on qingyuan pheasant intestinal flora.
1.6 data processing and statistical analysis
And (3) carrying out 16S rDNA high-throughput sequencing on the test chicken intestinal flora, then carrying out amplification and sequencing on the 1696 V3-V4 region, and analyzing the diversity and difference of the test chicken intestinal microorganisms based on the OTU and species annotation result. One-Way ANOVA program in SPSS 26.0 software was used for One-Way ANOVA, multiple comparisons were made by Duncan's method, data are expressed as "mean ± standard deviation", and P < 0.05 was used as the criterion for significance of difference.
Results and analysis
2.1 the effect of each test group on the growth performance of Qingyuan partridge chickens, the results are shown in Table 1.
TABLE 1 detection results of growth performance of Qingyuan partridge chickens
Note: the data in the same column has obvious difference in different shoulder mark letters (P is less than 0.05); shoulder marks same letter indicates that the difference is not significant (P > 0.05)
From table 1, it can be calculated that: at 21 days of age, the composite probiotic composition group is reduced by 1.66% compared with a control group, but the difference is not significant (P is more than 0.05); the ADG of the test chicken of the compound probiotic composition group and the antibiotic group is respectively improved by 2.11 percent and 6.47 percent compared with the control group, but the difference is not significant (P is more than 0.05); the F/G of the 2 groups of the test groups is respectively reduced by 4.02 percent and 3.45 percent compared with the control group, but the difference is not significant (P is more than 0.05). At 42 days of age, the ADFI of the test chicken in the composite probiotic composition group is reduced by 2.03 percent (P is more than 0.05) compared with that of the control group, and the ADFI of the antibiotic group is improved by 2.58 percent (P is more than 0.05) compared with that of the control group; the ADG of the test chicken in the compound probiotic composition group and the antibiotic group is respectively improved by 4.24 percent and 5.41 percent compared with the control group, but the difference is not significant (P is more than 0.05); F/G was reduced by 5.51% (P < 0.05) and 2.57% (P > 0.05) respectively, compared to the control group (Table 1). The result shows that the probiotics I can increase the daily gain of the Qingyuan partridge chickens, reduce the feed-weight ratio and improve the growth performance of the Qingyuan partridge chickens.
2.2 Effect of different additives on immune Performance of Qingyuan chickens
The results are shown in Table 2.
TABLE 2 detection results of immunity of Qingyuan pockmarked chicken
Table 2 results of immune performance of Qingyuan partridge chicken pg·mL -1
From table 2, it can be calculated that: the enzyme-linked immunosorbent assay (ELISA) is adopted to determine the content of IL-4 and IFN-gamma in the serum of the Qingyuan partridge chickens, and the content of IL-4 in the serum of the Qingyuan partridge chickens of the composite probiotic composition group is respectively increased by 31.88 percent and 18.93 percent (P is less than 0.05) compared with that of a control group and an antibiotic group; IFN-gamma of the composite probiotic composition group is reduced by 24.56 percent and 30.52 percent (P is less than 0.05) respectively compared with a control group and an antibiotic group; the IL-4 and IFN-gamma contents in the serum of the antibiotic group chicks are respectively improved by 4.28 percent and 8.58 percent (P is more than 0.05) compared with the control group (Table 2), which shows that the compound probiotics have the effects of improving the IL-4 in the serum of the remote-control partridges chicken and reducing the IFN-gamma content.
In conclusion, the addition of the composite probiotic composition can increase the daily gain of the Qingyuan pockmarked chicks, reduce the feed-weight ratio, improve the anti-inflammatory factor IL-4 in serum, and simultaneously reduce the level of the proinflammatory factor IFN-gamma, and the content of the IFN-gamma in the serum can be improved by feeding the compound probiotic composition with antibiotics. The addition of the composite probiotic composition can increase the abundance of cecal flora, is beneficial to the establishment of healthy intestinal flora of the Qingyuan partridge chickens, but the addition of antibiotics can reduce the microecological diversity of intestinal tracts, destroy the normal ratio of bacteroidetes and firmicutes and be not beneficial to the intestinal health of the chickens.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention.
Claims (8)
1. A composite probiotic composition for Qingyuan spicy-feather chicks is characterized in that: the composite probiotic composition is prepared from composite probiotic powder, taro powder, molasses, sodium glutamate and konjac glucomannan;
the composite probiotic powder is prepared from the following raw materials in parts by weight: 1-10 parts of bacillus subtilis, 1-5 parts of bacillus licheniformis, 2-7 parts of pediococcus acidilactici, 2-6 parts of lactobacillus, 1-5 parts of bifidobacterium longum and 1-5 parts of vibrio gulcomosus.
2. The complex probiotic composition for the Qingyuan pockmarked-feather chick according to claim 1, wherein: the number of viable bacteria of each probiotic in the composite probiotic powder is at least 2 x 10 9 cfu/g。
3. The complex probiotic composition for the Qingyuan pockmarked-feather chick according to claim 1, wherein: the composite probiotic powder is prepared from the following raw materials in parts by weight: 8 parts of bacillus subtilis, 4 parts of bacillus licheniformis, 3 parts of pediococcus acidilactici, 3 parts of lactobacillus, 2 parts of bifidobacterium longum and 3 parts of vibrio gulcomosus.
4. The complex probiotic composition for the Qingyuan pockmarked-feather chick according to claim 1, wherein: the composite probiotic composition comprises the following raw materials in parts by weight: 20-45 parts of composite probiotic powder, 30-50 parts of taro powder, 3-5 parts of molasses, 1-5 parts of sodium glutamate and 5-10 parts of konjac glucomannan.
5. The complex probiotic composition for the Qingyuan pockmarked-feather chick according to claim 4, wherein: the composite probiotic composition comprises the following raw materials in parts by weight: 35 parts of composite probiotic powder, 40 parts of taro powder, 4 parts of molasses, 3 parts of sodium glutamate and 8 parts of konjac glucomannan.
6. The method for preparing the complex probiotic composition for the Qingyuan pockmarked-feather chick according to any one of claims 1 to 5, wherein: the method comprises the following steps:
(1) Firstly, stirring and dissolving the taro powder with water at 50-80 ℃;
(2) Cooling to normal temperature, adding molasses, sodium glutamate and konjac glucomannan, and stirring;
(3) And finally, adding the composite probiotic powder into the dissolved solution, and stirring and mixing uniformly.
7. The method for preparing the composite probiotic composition for the Qingyuan pockmarked-feather chick according to claim 6, wherein the probiotic composition comprises the following components: the mass ratio of the taro powder to the water is 1:1-3.
8. The method for preparing the composite probiotic composition for the Qingyuan pockmarked-feather chick according to claim 6, wherein the probiotic composition comprises the following components: the purity of the taro powder is more than 98 percent.
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