CN109939211B - Traditional Chinese veterinary medicine preparation for treating chicken colibacillosis and preparation method thereof - Google Patents

Traditional Chinese veterinary medicine preparation for treating chicken colibacillosis and preparation method thereof Download PDF

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CN109939211B
CN109939211B CN201910336817.9A CN201910336817A CN109939211B CN 109939211 B CN109939211 B CN 109939211B CN 201910336817 A CN201910336817 A CN 201910336817A CN 109939211 B CN109939211 B CN 109939211B
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magnolia officinalis
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CN109939211A (en
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张启迪
梁晓
赵永达
刘宝涛
邹明
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Qingdao Agricultural University
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Abstract

The invention relates to a traditional Chinese veterinary medicine preparation for treating chicken colibacillosis, which comprises the following traditional Chinese medicinal materials in parts by weight: 10-20 parts of clove, 20-35 parts of magnolia officinalis, 10-20 parts of linden jacquard, 25-50 parts of duckweed and 10-15 parts of dried ginger. The prescription of the invention has mild property, the spleen invigorating, dampness eliminating, yang activating and diuresis inducing, and good antibacterial effect. The invention also provides a preparation method of the veterinary Chinese medicine preparation, which effectively improves the magnolol content and prevents the loss of the volatile oil. The effective components in the medicine are extracted most by three times of decoction under the condition of keeping the best efficacy of the prescription.

Description

Traditional Chinese veterinary medicine preparation for treating chicken colibacillosis and preparation method thereof
Technical Field
The invention relates to the field of traditional Chinese veterinary medicines for poultry, in particular to a traditional Chinese veterinary medicine preparation for treating chicken colibacillosis and a preparation method thereof.
Background
Colibacillosis in chickens is an acute and chronic bacterial infectious disease of various chickens caused by pathogenic escherichia coli, and can occur in chickens of various varieties and ages. In recent years, with the continuous large-scale development of the breeding industry, a plurality of farmers increase the breeding amount blindly, so that the breeding environment is worsened, colibacillosis is in a trend of increasing prevalence, and huge economic loss is caused to breeding production. Colibacillosis in chickens, which is an important bacterial disease harming the development of the chicken industry, generally has the following obstacles in prevention and treatment: firstly, antibiotic resistance; secondly, the medicine residue harms the food safety; and thirdly, the serotype is multiple, and the vaccine is difficult to prevent and treat. Therefore, the search for a safe, effective, drug-resistant and convenient-to-use drug for resisting chicken colibacillosis is of great importance in the chicken industry.
For the causes of chicken colibacillosis, Chinese veterinarians think that the chicken colibacillosis is pathogenic by damp pathogen, heat pathogen hurts liver, and heat pathogen hurts heart. The compendium of materia medica, forty-eight, birds and chicken says that the feather can not fly because of generation of chicken eggs, although it is yang essence, it belongs to Fengmu, and it is yin in yang. The physiological characteristics of chicken are yin in yang, preferring dryness and aversion to dampness, often deficiency of wei-defensive yang and weakness of lung qi. The general body temperature in the aspect of chicken's metabolism is higher, and the rhythm of the heart is fast, breathes soon, and present plant belongs to closed environment mostly, and the temperature is high humidity big, so the chicken is in the majority with damp and hot syndrome.
Clinical analysis shows that the phenomenon of urate deposition and edema of organs such as heart and liver are mostly seen. Long-term edema, blood stasis and dampness causing spleen and stomach disharmony, spleen qi failing to rise and stomach qi failing to fall, and spleen and stomach strengthening and edema elimination are needed during treatment. Therefore, invigorating spleen to eliminate dampness, activating yang to promote diuresis, and promoting qi circulation to remove water are the key points of the medicine for treating chicken colibacillosis.
The traditional Chinese veterinary medicine prescription commonly used in clinic emphasizes antibacterial property, mostly adopts medicines with bitter and cold properties, has large dosage and more prescription components, not only influences the health of animal organisms and reduces the medicine effect, but also delays the optimal treatment period. According to in vitro antibacterial tests, heat-clearing Chinese medicines (such as herba Andrographitis and Coptidis rhizoma) with bitter and cold properties are screened for treatment, which are easy to injure spleen and stomach, cause gastric cavity swelling and constipation, and seriously affect the treatment effect.
Disclosure of Invention
In order to solve the problem that serious side effects are caused by the fact that the traditional Chinese veterinary medicine prescription adopts bitter and cold heat-clearing traditional Chinese medicines, the invention aims to provide the traditional Chinese veterinary medicine preparation for treating chicken colibacillosis and the preparation method thereof, wherein the traditional Chinese veterinary medicine preparation has mild medicine properties, is capable of invigorating spleen to eliminate dampness, and is capable of activating yang to promote diuresis, and has a good antibacterial effect.
The technical scheme adopted by the invention for solving the technical problems is as follows: a traditional Chinese veterinary medicine preparation for treating chicken colibacillosis comprises the following traditional Chinese medicinal materials in parts by weight: 10-20 parts of clove, 20-35 parts of magnolia officinalis, 10-20 parts of linden jacquard, 25-50 parts of duckweed and 10-15 parts of dried ginger.
Further, the traditional Chinese veterinary medicine preparation comprises the following traditional Chinese medicinal materials in parts by weight: 10-15 parts of clove, 25-30 parts of mangnolia officinalis, 10-15 parts of linden jacquard, 30-40 parts of duckweed and 10-12 parts of dried ginger.
Preferably, the traditional Chinese veterinary medicine preparation comprises the following traditional Chinese medicinal materials in parts by weight: 15 parts of clove, 20 parts of magnolia officinalis, 10 parts of linden jacquard, 45 parts of duckweed and 10 parts of dried ginger.
Furthermore, the traditional Chinese veterinary medicine preparation also comprises 5-10 parts of dried orange peel by weight, and under the condition that the parts by weight of clove, linden jacquard, duckweed and dried ginger are not changed, the parts by weight of magnolia officinalis are correspondingly reduced by the parts by weight of the added dried orange peel.
Preferably, the traditional Chinese veterinary medicine preparation comprises the following traditional Chinese medicinal materials in parts by weight: 15 parts of clove, 15 parts of magnolia officinalis, 10 parts of linden jacquard, 40 parts of duckweed, 10 parts of dried ginger and 10 parts of dried orange peel.
Preferably, the traditional Chinese veterinary medicine preparation is oral liquid.
The preparation method of the traditional Chinese veterinary medicine preparation for treating chicken colibacillosis without dried orange peel comprises the following steps:
firstly, 1/10 parts of magnolia officinalis by weight are taken as dried ginger, then the dried ginger is added with water and decocted, and all magnolia officinalis is put into the ginger decoction and heated by fire until the ginger decoction is completely absorbed by the magnolia officinalis for standby;
secondly, adding all the clove, the linden jacquard, the duckweed and the rest of the dried ginger into water which is 9-11 times of the weight of all the medicinal materials to soak for 1 hour, then adding the magnolia officinalis in the first step, decocting for boiling, and then decocting for 1 hour with slow fire;
thirdly, filtering the traditional Chinese medicines in the second step, adding filter residues into water accounting for 70-80% of the weight of the water added in the second step, and decocting for 1 hour;
fourthly, filtering the traditional Chinese medicine in the third step, and adding water accounting for 60-70% of the weight of the water added in the third step for decocting for 1 hour;
and step five, combining the liquid medicines decocted for three times, standing overnight at room temperature, centrifuging to obtain supernatant, concentrating the liquid medicine to 1g/mL, sterilizing, and storing at 4 ℃ for later use.
The preparation method of the traditional Chinese veterinary medicine preparation containing dried orange peel for treating chicken colibacillosis comprises the following steps:
firstly, 1/10 parts of magnolia officinalis by weight are taken as dried ginger, then the dried ginger is added with water and decocted, and all magnolia officinalis is put into the ginger decoction and heated by fire until the ginger decoction is completely absorbed by the magnolia officinalis for standby;
secondly, adding all the clove, the linden jacquard, the duckweed and the rest of the dried ginger into water which is 9-11 times of the weight of all the medicinal materials to soak for 1 hour, then adding the magnolia officinalis in the first step, decocting for boiling, and then decocting for 1 hour with slow fire;
thirdly, filtering the traditional Chinese medicines in the second step, adding filter residues into water accounting for 70-80% of the weight of the water added in the second step, and decocting for 1 hour;
fourthly, filtering the traditional Chinese medicines in the third step, adding all the dried orange peels into filter residues, and adding water accounting for 60-70% of the weight of the water added in the third step for decocting for 1 hour;
and step five, combining the liquid medicines decocted for three times, standing overnight at room temperature, centrifuging to obtain supernatant, concentrating the liquid medicine to 1g/mL, sterilizing, and storing at 4 ℃ for later use.
The traditional Chinese medicine components of the invention have the following medicinal properties and pharmacology:
clove, pungent in flavor and warm in nature, has the functions of warming the middle-jiao and lowering the adverse flow of qi, tonifying the kidney and supporting yang. It enters spleen, stomach, lung and kidney meridians. Can be used for treating deficiency-cold of spleen and stomach. It is a good stomach-warming medicine, can relieve abdominal flatulence and enhance digestion, contains oleum Caryophylli, and has inhibitory effect on pathogenic fungi, staphylococcus, dysentery, Escherichia coli, etc.
The magnolia officinalis is warm in nature, enters spleen, stomach, lung and large intestine channels, can invigorate spleen and regulate qi, can tonify without stagnation, and can effectively relieve cold. Magnolol and honokiol in cortex Magnolia officinalis have strong antibacterial effect on gram-negative bacilli such as dysentery bacillus and Escherichia coli.
Tangerine peel, pericarpium Citri Reticulatae regulates qi to invigorate the spleen, eliminates dampness and phlegm, and controls qi downward to relieve asthma.
Bodhi flower, also known as King flower, promotes digestion, induces sweating and diuresis, relieves fever symptoms caused by diseases, and improves organism immunity.
The duckweed can induce diuresis, induce sweating, induce diuresis, clear heat and remove toxicity. The record of the materia medica shiyi includes: ' No-powder-application facial anhydride; when pounded, the decoction is mainly used for edema and diuresis; for people who are poisoned, the dried powder of the duckweed is taken with wine and the formula is an ancient type of spoon; but also as ointment for growing hair. "meridian tropism: the herbal Zhenzhen: entering liver and spleen; lei Gong processing pharmacology explanation: "enter the lung and small intestine meridians".
Gan Jiang can treat diarrhea due to middle cold and spleen-tonifying to resolve dampness. The book of Ben Jing records: "pungent and warm". Meridian tropism: enter spleen, stomach and lung meridians.
The formula adopts the dried ginger as an adjuvant drug of the duckweed, and can complement each other to help the lung ventilating, the urination inducing and the swelling dispersing. The bodhi jacquard is adopted as a ministerial drug to assist, and the bodhi jacquard is warm in nature, promotes digestion, sweating and diuresis, improves the immunity of the organism, so the bodhi jacquard, the duckweed and the dried ginger can strengthen the spleen and the stomach, promote diuresis and sweat, effectively relieve clinical symptoms such as edema and fever, and achieve the effects of strengthening the spleen, resolving dampness, activating yang, promoting diuresis, resolving qi and promoting diuresis.
Both clove and magnolia officinalis have the effects of tonifying spleen and regulating qi, the clove oil in clove and magnolol and honokiol in magnolia officinalis have strong inhibition effects on escherichia coli, and the clove and the magnolia officinalis are compatible to assist in treating escherichia coli diseases, can generate a synergistic enhancement effect, improve the antibacterial effect of the clove and the magnolia officinalis, and can improve the immunity of an organism; the magnolia bark and the dried orange peel are matched, so that the problems of spleen and stomach disharmony and stomach qi failing to descend caused by diseases can be effectively relieved, the appetite is increased, the treatment effect is improved, and both the symptoms and root causes are treated.
The prescription has the advantages that the taking amount of each medicinal material is precise and the dosage is proper, the functions of each component are fully exerted, and the compatibility of the medicinal materials can furthest exert the drug effect. The problems of excessive medicinal material types, reduced drug effect and great side effect do not exist.
The medicinal materials are based on the basic principles of treatment based on syndrome differentiation and overall conditioning, and the administration is based on the following, so that the aims of treating symptoms and root causes, improving the immunity and relieving various clinical symptoms of the colibacillosis are fulfilled, the aim of effective treatment is fulfilled, and almost no side effect is generated. In addition, the formula can improve the laying rate of the laying hens by regulating the stomach and the intestinal tracts of the laying hens, optimizing the intestinal flora and promoting the digestion and absorption functions.
The preparation method of the invention has the following beneficial effects:
the magnolia bark and the ginger are processed according to the weight ratio of 10:1, so that the problems of strong medicinal potency, spicy taste and irritation to esophagus of the magnolia bark are solved, the irritation can be eliminated after the magnolia bark and the ginger are processed, the effects of relieving epigastric distention and harmonizing stomach can be enhanced, the magnolol content is effectively improved, and the loss of volatile oil is prevented. The effective components in the medicine are extracted most by three times of decoction under the condition of keeping the best efficacy of the prescription. The tangerine peel is added in the last decoction for preventing the loss of the volatile oil in the tangerine peel from being too much and influencing the drug effect.
Detailed Description
Specific examples of the present invention are described below in conjunction with experimental and clinical results.
The first embodiment is as follows:
30g of clove, 50g of mangnolia officinalis, 20g of linden jacquard, 80g of duckweed and 20g of dried ginger are taken to prepare liquid medicine according to the following steps:
firstly, adding 5g of dried ginger into water to decoct, putting 50g of magnolia officinalis into the ginger decoction, heating with Chinese fire until the ginger decoction is completely absorbed by the magnolia officinalis for later use. The magnolia officinalis is processed by the ginger, so that the problems of strong medicinal potency, spicy taste and irritation to throat are solved, the irritation can be eliminated after the magnolia officinalis is processed by the ginger, the effects of relieving the epigastric distention and harmonizing the stomach can be enhanced, the magnolol content is effectively improved, and the loss of volatile oil is prevented.
And secondly, adding 30g of clove, 20g of linden jacquard, 80g of duckweed and 15g of dried ginger into 2000ml of water for soaking for 1h, then adding the magnolia officinalis obtained in the first step, decocting the mixture for boiling, and then decocting the mixture for 1h with slow fire.
And step three, filtering the traditional Chinese medicines in the step two, and adding 1500ml of water into filter residues to decoct for 1 hour.
And fourthly, filtering the traditional Chinese medicine in the third step, and adding 1000ml of water into filter residues to decoct for 1 hour.
And step five, combining the liquid medicines decocted for three times, standing overnight at room temperature, centrifuging to obtain supernatant, concentrating the liquid medicine to 1g/mL, sterilizing, and storing at 4 ℃ for later use.
The veterinary drug oral liquid has the test effects on the artificial induction of chicken colibacillosis:
1 preparation of bacterial liquid
The E.coli under storage was recovered and propagated in LB broth. Ten times of dilution method is adopted, and the bacteria liquid is prepared by mixing evenly. And (3) putting 1mL of the bacterial liquid into a plate, injecting about 15mL of nutrient agar, uniformly mixing, solidifying and then culturing at 37 ℃ for 18 h.
2 establishment of chicken colibacillus model and determination of toxic counteracting dose
The pectoralis muscles of 25-day-old Holland brown chickens were injected with 0.2mL, 0.3mL, 0.4mL, 0.5mL of 4.8X 108cfu/mL of bacterial suspension, and 10 were inoculated per dose. And (5) recording the morbidity and mortality of the test chicken, and determining the toxic counteracting dose of the formal test. After 6h of toxin counteracting, the tested chickens show decreased appetite, depression, drainage-like feces, death of the tested chickens begins to occur after 18h, and the morbidity and mortality are counted after 4 days, wherein the morbidity of a pectoralis injection group with 0.3mL is 90%, the mortality is 70%, and 0.3mL of bacterial liquid is selected as the toxin counteracting dose of a clinical efficacy test.
3 test of drug efficacy
3.1 animal grouping
The chickens are raised in cages conventionally and fed with free drinking water. 120 healthy chicks of 25d age are selected, weighed and randomly divided into 4 groups, namely a Chinese veterinary drug group, a drug control group, a toxicity attacking control group and a blank control group, and after 0.3mL of bacteria are inoculated to each group of chicks for outbreak, the group is respectively administered according to the table 1.1, 1 time in the morning and afternoon of each day and 5d is continuously used. The clinical symptoms of the chicken flocks are observed during the medication period, and the feed intake, death, cure, obvious effect and effective conditions of all groups of chickens are recorded. The groups of test chickens and the dosing schedule are shown in table 1.1.
TABLE 1.1 groups of test chickens and dosing regimens
3.2 test index and statistical analysis method
And (3) curing: during the test period, clinical symptoms basically disappeared after the administration, and the spirit and ingestion were recovered to normal.
The effect is shown: during the test period, the clinical symptoms after the administration are obviously relieved, and the spirit and the ingestion are basically recovered.
The method has the following advantages: during the trial, clinical symptoms were reduced after administration, but mental and feeding status was not substantially restored.
And (4) invalidation: death occurred during the trial, and no reduction in clinical symptoms or death of the diseased chicken was seen.
The total effective rate is as follows: after the test is finished, the sum of the cure number, the effect number and the effective number of the chicken flocks accounts for the percentage of the treatment number of the chicken flocks.
Statistical analysis method
X for the total efficiency2(chi fang) inspection.
4 results of curative effect of each group of clinical drug effect on artificially infected chicken colibacillosis
The specific results of the clinical efficacy of each group of the artificially infected chicken colibacillosis are shown in Table 1.2. As can be seen from table 1.2, the cure numbers of the traditional Chinese veterinary medicine group and the drug control group are higher than those of the toxicity attacking control group, and the difference between the traditional Chinese veterinary medicine group and the drug control group is not large; the total effective rate of the control group for counteracting toxic substance is significantly different from that of the Chinese veterinary medicine group and the medicine control group (P <0.05), and the difference of the treatment effect of the Chinese veterinary medicine group and the medicine control group is not significant (P > 0.05). Therefore, the veterinary drug developed by the invention can effectively reduce the mortality of escherichia coli, and the effect is equivalent to that of a drug control group.
TABLE 1.2 results of therapeutic effect of each group of clinical drug effect on colibacillosis of artificially infected chickens
Note: the same lower case letters in the same column data indicate no significant difference (P >0.05), and the different lower case letters indicate significant difference (P < 0.05). The cure number in the challenge control group and the blank control group was the survival number. The same applies below.
Example two:
30g of clove, 40g of mangnolia officinalis, 20g of linden jacquard, 90g of duckweed and 20g of dried ginger are taken, and the liquid medicine is prepared according to the following steps:
firstly, 4g of dried ginger is taken and added with water to be decocted, 40g of magnolia officinalis is put into the ginger decoction and heated with Chinese fire until the ginger decoction is completely absorbed by the magnolia officinalis for later use.
And secondly, adding 30g of clove, 20g of linden jacquard, 90g of duckweed and 16g of dried ginger into 2000ml of water, soaking for 1h, then adding the magnolia officinalis obtained in the first step, decocting for boiling, and then decocting for 1h with slow fire.
And step three, filtering the traditional Chinese medicines in the step two, and adding 1500ml of water into filter residues to decoct for 1 hour.
And fourthly, filtering the traditional Chinese medicine in the third step, and adding 1000ml of water into filter residues to decoct for 1 hour.
And step five, combining the liquid medicines decocted for three times, standing overnight at room temperature, centrifuging to obtain supernatant, concentrating the liquid medicine to 1g/mL, sterilizing, and storing at 4 ℃ for later use.
The veterinary drug oral liquid has the test effects on the artificial induction of chicken colibacillosis:
1 bacterial liquid preparation, establishment of a chicken colibacillus model and determination of the toxicity attacking dose are the same as in the first embodiment.
2 test of drug efficacy
2.1 animal groups
The chickens are raised in cages conventionally and fed with free drinking water. 120 healthy chicks of 25d age are selected, weighed and randomly divided into 4 groups, namely a Chinese veterinary drug group, a drug control group, a toxicity attacking control group and a blank control group, and after 0.3mL of bacteria are inoculated to each group of chicks for outbreak, the group is respectively administered according to the table 2.1, 1 time in the morning and afternoon of each day and 5d is continuously used. The clinical symptoms of the chicken flocks are observed during the medication period, and the feed intake, death, cure, obvious effect and effective conditions of all groups of chickens are recorded. The groups of test chickens and the dosing schedule are shown in table 2.1.
TABLE 2.1 groups of test chickens and dosing regimens
2.2 test index and statistical analysis method
And (3) curing: during the test period, clinical symptoms basically disappeared after the administration, and the spirit and ingestion were recovered to normal.
The effect is shown: during the test period, the clinical symptoms after the administration are obviously relieved, and the spirit and the ingestion are basically recovered.
The method has the following advantages: during the trial, clinical symptoms were reduced after administration, but mental and feeding status was not substantially restored.
And (4) invalidation: death occurred during the trial, and no reduction in clinical symptoms or death of the diseased chicken was seen.
The total effective rate is as follows: after the test is finished, the sum of the cure number, the effect number and the effective number of the chicken flocks accounts for the percentage of the treatment number of the chicken flocks.
Statistical analysis method
X for the total efficiency2(chi fang) inspection.
3 results of curative effect of each group of clinical drug effect on artificially infected chicken colibacillosis
The specific results of the clinical efficacy of each group of the artificially infected chicken colibacillosis are shown in Table 2.2. As can be seen from table 2.2, the cure numbers of the traditional Chinese veterinary medicine group and the drug control group are higher than those of the toxicity attacking control group, and the traditional Chinese veterinary medicine group and the drug control group have little difference, but the effect is inferior to that of the traditional Chinese veterinary medicine in the first embodiment; the total effective rate of the control group for counteracting toxic substance is significantly different from that of the Chinese veterinary medicine group and the medicine control group (P <0.05), and the difference of the treatment effect of the Chinese veterinary medicine group and the medicine control group is not significant (P > 0.05). Therefore, the veterinary drug developed by the invention can effectively reduce the mortality rate of escherichia coli.
TABLE 2.2 results of therapeutic effect of each group of clinical drug effect on colibacillosis of artificially infected chickens
Example three:
30g of clove, 30g of mangnolia officinalis, 20g of linden jacquard, 80g of duckweed, 20g of dried ginger and 20g of dried orange peel are taken, and the liquid medicine is prepared according to the following steps:
firstly, 3g of dried ginger is taken and added with water to be decocted, 30g of magnolia officinalis is put into the ginger decoction and heated by slow fire until the ginger decoction is completely absorbed by the magnolia officinalis for standby.
And secondly, adding 30g of clove, 20g of linden jacquard, 80g of duckweed and 17g of dried ginger into 2000ml of water, soaking for 1h, then adding the magnolia officinalis obtained in the first step, decocting for boiling, and then decocting for 1h with slow fire.
And step three, filtering the traditional Chinese medicines in the step two, and adding 1500ml of water into filter residues to decoct for 1 hour.
And fourthly, filtering the traditional Chinese medicines in the third step, adding 20g of dried orange peel into filter residues, and adding 1000ml of water for decocting for 1 hour. The tangerine peel is added in the step to prevent the loss of the volatile oil in the tangerine peel from being too much and influence the drug effect.
And step five, combining the liquid medicines decocted for three times, standing overnight at room temperature, centrifuging to obtain supernatant, concentrating the liquid medicine to 1g/mL, sterilizing, and storing at 4 ℃ for later use.
The veterinary drug oral liquid has the test effects on the artificial induction of chicken colibacillosis:
1 bacterial liquid preparation, establishment of a chicken colibacillus model and determination of the toxicity attacking dose are the same as in the first embodiment.
2 test of drug efficacy
2.1 animal groups
The chickens are raised in cages conventionally and fed with free drinking water. 120 healthy chicks of 25d age are selected, weighed and randomly divided into 4 groups, namely a Chinese veterinary drug group, a drug control group, a toxicity attacking control group and a blank control group, and after 0.3mL of bacteria are inoculated to each group of chicks for outbreak, the group is respectively administered according to the table 3.1, 1 time in the morning and afternoon of each day and 5d is continuously used. The clinical symptoms of the chicken flocks are observed during the medication period, and the feed intake, death, cure, obvious effect and effective conditions of all groups of chickens are recorded. The groups of test chickens and the dosing schedule are shown in table 3.1.
TABLE 3.1 groups of test chickens and dosing regimens
2.2 test index and statistical analysis method
And (3) curing: during the test period, clinical symptoms basically disappeared after the administration, and the spirit and ingestion were recovered to normal.
The effect is shown: during the test period, the clinical symptoms after the administration are obviously relieved, and the spirit and the ingestion are basically recovered.
The method has the following advantages: during the trial, clinical symptoms were reduced after administration, but mental and feeding status was not substantially restored.
And (4) invalidation: death occurred during the trial, and no reduction in clinical symptoms or death of the diseased chicken was seen.
The total effective rate is as follows: after the test is finished, the sum of the cure number, the effect number and the effective number of the chicken flocks accounts for the percentage of the treatment number of the chicken flocks.
Statistical analysis method
X for the total efficiency2(chi fang) inspection.
3 results of curative effect of each group of clinical drug effect on artificially infected chicken colibacillosis
The specific results of the clinical efficacy of the artificial infected chicken colibacillosis are shown in Table 3.2. As can be seen from table 3.2, the cure numbers of the traditional Chinese veterinary medicine group and the drug control group are higher than those of the toxicity attacking control group, and the difference between the traditional Chinese veterinary medicine group and the drug control group is not large; the total effective rate of the control group for counteracting toxic substance is significantly different from that of the Chinese veterinary medicine group and the medicine control group (P <0.05), and the difference of the treatment effect of the Chinese veterinary medicine group and the medicine control group is not significant (P > 0.05). Therefore, the traditional Chinese veterinary medicine developed by the invention can effectively reduce the mortality of escherichia coli, has the effect equivalent to that of a medicine control group, and achieves the treatment effect equivalent to that of florfenicol.
TABLE 3.2 results of therapeutic effect of each group of clinical drug effect on colibacillosis of artificially infected chickens
Tests of the three examples show that the prescription and the drug control group in the first example and the second example can effectively reduce the mortality rate of escherichia coli, and the difference is not obvious, but the prescription of 15 parts of clove, 15 parts of magnolia officinalis, 10 parts of linden jacquard, 40 parts of duckweed, 10 parts of dried ginger and 10 parts of dried orange peel in the example 3 has the best treatment effect group on chicken escherichia coli, and the total effective rate is higher than that of the first example and the second example and is basically close to the treatment effect of florfenicol.
Example of clinical test
In one farmer in the cold pavilion area of Weifang city in Shandong province, 8000 white feather broilers were bred, 20-day-old chickens appeared as listlessness, decreased or no intake, and the cesarean chickens showed pale conjunctiva, thickened peritoneum, enlarged liver, surface with cellulose exudation, thickened pericardium, yellow-white cellulose, and symptoms of air sacculitis, pericarditis, peritonitis, etc. The chicken liver smear is taken, gram staining is carried out, gram negative bacilli are obtained through microscopic examination, the bacteria grow well in a common culture medium, the surface of a bacterial colony is smooth, opaque and glossy, the bacteria grow well in Mackanka, the surface of the bacterial colony is smooth and moist, the edge is neat, and the chicken colibacillosis is judged. There were 578 cases and 56 deaths. The remaining 522 sick chickens drink water to the chickens according to 6mL/kg, the traditional Chinese veterinary medicine preparation disclosed by the third embodiment of the invention is added, the effect of 58 chickens is poor after the continuous feeding for 5 days, 36 chickens die after five days, 428 has a better treatment effect, the sick chickens basically recover to be normal, and the total effective rate reaches 82%.

Claims (7)

1. The traditional Chinese veterinary medicine preparation for treating chicken colibacillosis is characterized by being prepared from the following traditional Chinese medicinal materials in parts by weight: 10-20 parts of clove, 20-35 parts of magnolia officinalis, 10-20 parts of linden jacquard, 25-50 parts of duckweed and 10-15 parts of dried ginger.
2. The traditional Chinese veterinary medicine preparation for treating chicken colibacillosis according to claim 1, which is prepared from the following traditional Chinese medicinal materials in parts by weight: 10-15 parts of clove, 25-30 parts of mangnolia officinalis, 10-15 parts of linden jacquard, 30-40 parts of duckweed and 10-12 parts of dried ginger.
3. The traditional Chinese veterinary medicine preparation for treating chicken colibacillosis according to claim 1, which is prepared from the following traditional Chinese medicinal materials in parts by weight: 15 parts of clove, 20 parts of magnolia officinalis, 10 parts of linden jacquard, 45 parts of duckweed and 10 parts of dried ginger.
4. The traditional Chinese veterinary medicine preparation for treating chicken colibacillosis is characterized by being prepared from the following traditional Chinese medicinal materials in parts by weight: 15 parts of clove, 15 parts of magnolia officinalis, 10 parts of linden jacquard, 40 parts of duckweed, 10 parts of dried ginger and 10 parts of dried orange peel.
5. The veterinary Chinese medicine preparation for treating chicken colibacillosis according to claim 1, wherein the veterinary Chinese medicine preparation is an oral liquid.
6. The veterinary drug formulation for treating chicken colibacillosis as claimed in claim 1 or 2 or 3 or 5, which is prepared by the following steps:
firstly, 1/10 parts of magnolia officinalis by weight are taken as dried ginger, then the dried ginger is added with water and decocted, and all magnolia officinalis is put into the ginger decoction and heated by fire until the ginger decoction is completely absorbed by the magnolia officinalis for standby;
secondly, adding all the clove, the linden jacquard, the duckweed and the rest of the dried ginger into water which is 9-11 times of the weight of all the medicinal materials to soak for 1 hour, then adding the magnolia officinalis in the first step, decocting for boiling, and then decocting for 1 hour with slow fire;
thirdly, filtering the traditional Chinese medicines in the second step, adding filter residues into water accounting for 70-80% of the weight of the water added in the second step, and decocting for 1 hour;
fourthly, filtering the traditional Chinese medicine in the third step, and adding water accounting for 60-70% of the weight of the water added in the third step for decocting for 1 hour;
and step five, combining the liquid medicines decocted for three times, standing overnight at room temperature, centrifuging to obtain supernatant, concentrating the liquid medicine to 1g/mL, sterilizing, and storing at 4 ℃ for later use.
7. The veterinary drug formulation for treating chicken colibacillosis as claimed in claim 4, which is prepared by the following steps:
firstly, 1/10 parts of magnolia officinalis by weight are taken as dried ginger, then the dried ginger is added with water and decocted, and all magnolia officinalis is put into the ginger decoction and heated by fire until the ginger decoction is completely absorbed by the magnolia officinalis for standby;
secondly, adding all the clove, the linden jacquard, the duckweed and the rest of the dried ginger into water which is 9-11 times of the weight of all the medicinal materials to soak for 1 hour, then adding the magnolia officinalis in the first step, decocting for boiling, and then decocting for 1 hour with slow fire;
thirdly, filtering the traditional Chinese medicines in the second step, adding filter residues into water accounting for 70-80% of the weight of the water added in the second step, and decocting for 1 hour;
fourthly, filtering the traditional Chinese medicines in the third step, adding all the dried orange peels into filter residues, and adding water accounting for 60-70% of the weight of the water added in the third step for decocting for 1 hour;
and step five, combining the liquid medicines decocted for three times, standing overnight at room temperature, centrifuging to obtain supernatant, concentrating the liquid medicine to 1g/mL, sterilizing, and storing at 4 ℃ for later use.
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