CN111110772A - Solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites - Google Patents
Solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/11—Pteridophyta or Filicophyta (ferns)
- A61K36/12—Filicopsida or Pteridopsida
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/13—Coniferophyta (gymnosperms)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/889—Arecaceae, Palmae or Palmaceae (Palm family), e.g. date or coconut palm or palmetto
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/10—Anthelmintics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/14—Ectoparasiticides, e.g. scabicides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention discloses a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites, which belongs to the technical field of fermentation traditional Chinese medicine additives and is prepared by the following preparation method: mixing radix Sophorae Flavescentis extract, herba Artemisiae Scopariae extract, herba et Gemma Agrimoniae extract, radix Dichroae extract, rhizoma Osmundae extract, Arecae semen extract, semen Torreyae extract, semen Cucurbitae extract 15 and Glycyrrhrizae radix extract to obtain mixed powder; uniformly mixing the mixed powder with a fermentation medium, inoculating probiotics, fermenting, and spray-drying to obtain fermented mixture powder; packaging and warehousing; the invention takes the traditional Chinese medicine extract as the fermentation raw material, and utilizes the optimized saccharomyces cerevisiae and bacillus licheniformis to carry out biotransformation on the intermediate substance to prepare the traditional Chinese medicine microecological preparation which has the advantages of no toxicity, no residue, greenness, easy absorption and obviously enhanced antibacterial activity, so that the drug effect is obviously improved.
Description
Technical Field
The invention relates to the technical field of fermented traditional Chinese medicine additives, in particular to a solid-state fermented traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites.
Background
The parasitic diseases of livestock and poultry are diseases caused by various pathogenic parasites parasitizing on animals and poultry, including ① the diseases caused by helminth parasitizing on livestock and poultry, which are helminthiasis, frequently occurring fasciolosis of livers of cattle and sheep, alimentary canal nematodosis, taeniasis, echinococcosis, cysticercosis (commonly known as micturition), pulmonary filariasis and the like ② ectoparasites, mainly including some insects, such as mosquitoes, sand flies, lice, fleas, ticks and the like, when the insects stab blood, the insects contact the body surface of a host and leave after sucking blood, ③ the diseases caused by the protozoa parasitizing on livestock and poultry, which are frequently occurring coking diseases, chicken coccidiosis and the like.
① causes animal death, most parasitic diseases are chronic, but some parasites are epidemic in some areas and cause acute morbidity and mortality of animals, such as chicken, rabbit coccidiosis, swine toxoplasmosis, etc. ② reduces animal productivity, parasitic diseases can obviously reduce animal productivity, for example, after piglets are infected with ascariasis, the weight gain is reduced by 30% compared with healthy pigs, and severe pigs can develop and stagnate to form "stiff pigs", even die, ③ influences animal growth, young animals are susceptible to the parasitic diseases to prevent growth and development, female animals are abnormally estrated due to malnutrition after being infected with parasites by breeding animals, the mating rate and conception rate are influenced, pregnant women are prone to abortion, premature birth or dead fetus ④ animal products are discarded, some parasitic meat and viscera are not utilized according to relevant regulations of veterinary health quarantine, and for example, the livestock are seriously infected with cysticercosis, echinococcosis, coccidiosis, liver and viscera of livestock are discarded, and the lung diseases are larger than the visceral diseases of the livestock.
Currently used antiparasitic agents include: anthelmintics (nematocides, cestodes, anthelmintics); anti-body surface parasitic agent (organic phosphorus, pyrethrin); antiprotozoal agents (anticoccidial, antitrypanosomal, antitrichomonal, or anticoccidial agents).
However, these antiparasitic drugs have high toxicity to livestock and poultry and much drug residue. In addition, the abuse problem in mass production causes the problems of increased drug resistance, poor treatment effect and the like. Therefore, the solid state fermentation traditional Chinese medicine preparation for preventing and controlling the parasites of the livestock and the poultry is provided.
Disclosure of Invention
The invention aims to provide a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites, and solves the problems of high toxicity, more drug residues, high drug resistance and poor treatment effect of the existing antiparasitic drugs on livestock and poultry.
In order to solve the technical problems, the invention provides the following technical scheme:
a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is prepared by the following preparation method:
s1: taking 30-40 parts of radix sophorae flavescentis extract, 25-35 parts of oriental wormwood extract, 25-35 parts of hairyvein agrimonia herb and bud extract, 25-35 parts of antifebrile dichroa extract, 20-30 parts of cyrtomium rhizome extract, 20-30 parts of betel nut extract, 20-30 parts of Chinese torreya seed extract, 15-25 parts of pumpkin seed extract and 15-25 parts of liquorice extract according to parts by weight to form mixed powder;
s2: uniformly mixing the mixed powder with a fermentation culture medium, inoculating probiotics, fermenting at 36-38 ℃ for 24-48 h to obtain a liquid traditional Chinese medicine fermentation mixture, and performing spray drying at 50-65 ℃ to obtain fermentation mixture powder;
s3: and subpackaging the solid fermentation mixture and warehousing.
Wherein, preferably, the formula of the fermentation medium in S2 is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5g of glucose, 1 g of soluble starch, 5g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water, mixing, adjusting the pH value to 6-6.5 by using 10% sodium hydroxide solution, and sterilizing at 121 ℃ for 15min under high pressure.
Wherein, preferably, the probiotics in the S2 are saccharomyces cerevisiae and bacillus licheniformis.
Preferably, the probiotic inoculated in S2 is a secondary seed solution of probiotic strains, and the preparation method of the secondary seed solution comprises the following steps:
(1) activating probiotic bacteria, inoculating into seed culture medium (10 times)9Probiotic bacteria/500 ml culture medium) is statically cultured for 12 h-24 h at 36 ℃ -38 ℃ to obtain first-stage seed liquid;
(2) inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2-10 vol%, and performing static culture at 36-38 ℃ for 12-24 h to obtain a secondary seed solution; wherein, preferably, the second-stage seed liquid in the S2 and the fermentation medium are inoculated according to the volume ratio of 3 vol% to 8 vol%.
Preferably, the formula of the seed culture medium for preparing the first-stage seed liquid and the second-stage seed liquid is as follows: 10 g of refined peptone, 5g of yeast extract, 20g of glucose, 5g of sodium acetate, 2 g of ammonium citrate, 0.1 g of tween 80, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
Wherein, the pressure intensity in the S2 fermentation is preferably 0.02 kPa-0.1 kPa, the stirring speed is 10 r/min-30 r/min, and the pH value is preferably 6.0-7.0.
Preferably, the adding proportion of the mixed powder and the fermentation medium in the S2 is (8-20) g: 1L of the compound.
The product is refined by adopting the technology of traditional Chinese medicine cell wall breaking and yeast complexing, improves the molecular activity of the traditional Chinese medicine extract, ensures that the extract is easier to absorb, has high bioavailability, ensures that bacillus licheniformis consumes a large amount of oxygen in intestinal tracts, causes the body of the insect to die by anoxia asphyxia, and greatly enhances the insect expelling effect. ① effectively expels various parasites in vivo and in vitro, such as ascaris suum, tapeworm, hookworm, filarial pulmonary worm, scabies, tick, louse and the like, ② effectively expels eperythrozoon, toxoplasma, coke worm, plasmodium and the like, ③ improves the production performance of animals, improves the feed reward, ④ resists anemia, stress and promotes growth.
The mechanism of antiparasitic action is as follows: as early as two thousand years ago, more than 30 kinds of insect repellents including dichroa, cyrtomium rhizome, omphalia, fructum furfurescence and the like are already introduced in the first pharmacopoeia of Shennong's herbal Jing in ancient China, and are the earliest recorded insect repellents (killers) in the world. Many Chinese herbs such as Juying, Bing Lang, nan Gua Zi, pomegranate root, Franze and wolf tooth are still used clinically.
Radix Sophorae Flavescentis (Sophora flavescens A i.t) is named as Maackia amurensis, ramulus Et folium Picrasmae, herba Ardisiae Japonicae, and Sophora japonica. Is a plant of the genus Sophora of the family Leguminosae, deciduous shrubs, is about 1-3 m high, and grows on hillsides, bushes and river bank sand lands. The root contains various alkaloids such as matrine, oxymatrine, hydroxymatrine, and allomatrine. The root of lightyellow sophora root is used as a medicine and has the effects of clearing away heat and toxic materials, eliminating dampness and killing insects. The Chinese herbal medicine matrine aqua is used as an animal external pesticide, can effectively kill ectoparasite pig scabies mites and pig blood lice on the body surface of animals, and has the characteristics of simple preparation, convenient use, high efficiency and no toxicity.
The Arecae semen is mature seed of Arecae semen of Palmae of Catechu L. Mainly produced in the areas of Hainan, Taiwan Guangxi, Yunnan, Fujian, etc.
Chemical components of betel nuts: contains 0.3-0.6% of alkaloid, mainly arecoline, and the rest is arecoline, demethylarecoline, arecoline, homoarecoline, isodemethylarecoline, etc., which are combined with tannic acid. The fat oil contains 14% of fatty acid such as lauric acid, myristic acid, palmitic acid, tetradecenoic acid, oleic acid, linoleic acid, stearic acid, etc. It also contains tannin and areca red pigment.
Areca catechu can cause delayed paralysis of tapeworm, and if touching, the worm will stretch out and be difficult to break, so that whole worm can be expelled; the arecoline has stronger paralysis effect on taenia solium, can paralyze all parts of the whole worm, and can only paralyze the head knot and immature segments of beef taenia; the Arecae semen has effects of treating and killing ascaris, pinworm, liver fluke, hookworm, and schistosome; has inhibitory effect on dermatophytes, influenza virus, and helicobacter pylori; arecoline has the effects of simulating choline, exciting choline receptors, promoting secretion of saliva and sweat glands, increasing intestinal peristalsis, slowing down heart rate and reducing blood pressure, and eye drops can shrink pupils.
Pumpkin seeds are seeds of Cucurbita moschata (Duch.) poiret, a plant belonging to cucurbitaceae. Mainly produced in Zhejiang, Jiangxi, Hunan, Hubei, Sichuan and other places. Chemical components: contains cucurbitine as effective component for expelling parasites. In addition, it contains fatty oil, protein, vitamins A, B1, B2, C, and carotene. The main components of the fatty oil are linseed oil acid, oleic acid, stearic acid and the like.
The pumpkin seeds have paralysis effect on the middle segment and the rear segment of the beef tapeworm or the pork tapeworm and have synergistic effect with the betel nut; it has effects of inhibiting and killing schistosome larva, and can make adult insect body atrophy, genital degeneration and intrauterine egg decrease, but not kill.
The rhizoma Osmundae is dried rhizome with petiole base of Dryopteris crassirhizophila Nakai of Dryopteridaceae. Mainly produced in mountain areas of Heilongjiang, Jilin and Liaoning provinces. The product mainly contains aspidin, tridentate fern, xanthocerate, aspic acid, volatile oil, aspidin, etc.
When the decoction of rhizome and petiole base of rhizoma Osmundae is diluted to 16%, it has different degrees of inhibiting and relaxing effect on head of ascaris suum in vitro. After 50% -70% of decoction acts on the whole ascaris suum for 2-6 hours, the activity of the ascaris suum is inhibited to different degrees.
Compared with the prior art, the invention has the beneficial effects that:
the traditional Chinese medicine fermentation preparation provided by the invention has the advantages that the preparation method is simple and reasonable, the traditional Chinese medicine is fermented, the advantages of the traditional Chinese medicine and the probiotics are combined, the production period is short, and the cost is low.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The embodiment provides a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites, which is prepared by the following preparation method:
s1: taking 35 parts of radix sophorae flavescentis extract, 30 parts of oriental wormwood extract, 30 parts of hairyvein agrimony extract, 30 parts of antifebrile dichroa extract, 25 parts of cyrtomium rhizome extract, 25 parts of betel nut extract, 25 parts of Chinese torreya seed extract, 20 parts of pumpkin seed extract and 20 parts of liquorice extract according to parts by weight to form mixed powder;
s2: uniformly mixing the mixed powder and a fermentation culture medium, inoculating probiotics, fermenting for 36 hours at the temperature of 36-38 ℃, the pressure of 0.05kPa, the stirring speed of 20r/min and the pH value of 6.5 to obtain a liquid traditional Chinese medicine fermentation mixture, and performing spray drying at the temperature of 60 ℃ to obtain fermentation mixture powder; wherein the adding proportion of the mixed powder and the fermentation medium is 15 g: 1L;
s3: and subpackaging the solid fermentation mixture and warehousing.
Wherein the formula of the fermentation medium in the S2 is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5g of glucose, 1 g of soluble starch, 5g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water, mixing, adjusting the pH value to 6-6.5 by using 10% sodium hydroxide solution, and sterilizing at 121 ℃ for 15min under high pressure.
Wherein the probiotics inoculated in the S2 is a secondary seed liquid of saccharomyces cerevisiae and bacillus licheniformis strains, the volume ratio of the secondary seed liquid to the fermentation culture medium is 5 vol%, and the preparation steps of the secondary seed liquid are as follows:
(1) activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 18 hours at 36-38 ℃ to obtain a primary seed solution; wherein the addition ratio of Saccharomyces cerevisiae in seed culture medium is 5 × 108cfu/ml, Bacillus licheniformis 5X 108cfu/ml；
(2) Inoculating the first-stage seed liquid into a seed culture medium according to the inoculation amount of 6 vol%, and performing static culture at 36-38 ℃ for 18h to obtain a second-stage seed liquid.
Wherein the formula of the seed culture medium for preparing the first-stage seed liquid and the second-stage seed liquid is as follows: 10 g of refined peptone, 5g of yeast extract, 20g of glucose, 5g of sodium acetate, 2 g of ammonium citrate, 0.1 g of tween 80, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
Example 2
The embodiment provides a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites, which is prepared by the following preparation method:
s1: taking 30 parts of radix sophorae flavescentis extract, 35 parts of oriental wormwood extract, 25 parts of hairyvein agrimony extract, 35 parts of antifebrile dichroa extract, 20 parts of cyrtomium rhizome extract, 30 parts of betel nut extract, 20 parts of Chinese torreya seed extract, 25 parts of pumpkin seed extract and 15 parts of liquorice extract according to parts by weight to form mixed powder;
s2: uniformly mixing the mixed powder and a fermentation culture medium, inoculating probiotics, fermenting for 48 hours at the temperature of 36-38 ℃, the pressure of 0.02kPa, the stirring speed of 30r/min and the pH value of 6.0 to obtain a liquid traditional Chinese medicine fermentation mixture, and performing spray drying at the temperature of 50 ℃ to obtain fermentation mixture powder; wherein the adding proportion of the mixed powder and the fermentation medium is 20 g: 1L;
s3: and subpackaging the solid fermentation mixture and warehousing.
Wherein the formula of the fermentation medium in the S2 is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5g of glucose, 1 g of soluble starch, 5g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water, mixing, adjusting the pH value to 6-6.5 by using 10% sodium hydroxide solution, and sterilizing at 121 ℃ for 15min under high pressure.
Wherein the probiotics inoculated in the S2 is a secondary seed liquid of saccharomyces cerevisiae and bacillus licheniformis strains, the volume ratio of the secondary seed liquid to the fermentation culture medium is 3 vol%, and the preparation steps of the secondary seed liquid are as follows:
(1) activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 12 hours at 36-38 ℃ to obtain a primary seed solution; wherein the addition ratio of Saccharomyces cerevisiae in seed culture medium is 5 × 108cfu/ml, Bacillus licheniformis 5X 108cfu/ml；
(2) Inoculating the first-stage seed liquid into a seed culture medium according to the inoculation amount of 2 vol%, and performing static culture at 36-38 ℃ for 24h to obtain a second-stage seed liquid.
Wherein the formula of the seed culture medium for preparing the first-stage seed liquid and the second-stage seed liquid is as follows: 10 g of refined peptone, 5g of yeast extract, 20g of glucose, 5g of sodium acetate, 2 g of ammonium citrate, 0.1 g of tween 80, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
Example 3
The embodiment provides a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites, which is prepared by the following preparation method:
s1: taking 40 parts of radix sophorae flavescentis extract, 25 parts of oriental wormwood extract, 35 parts of hairyvein agrimony extract, 25 parts of antifebrile dichroa extract, 30 parts of cyrtomium rhizome extract, 20 parts of betel nut extract, 30 parts of Chinese torreya seed extract, 15 parts of pumpkin seed extract and 25 parts of liquorice extract according to parts by weight to form mixed powder;
s2: uniformly mixing the mixed powder and a fermentation culture medium, inoculating probiotics, fermenting for 24 hours at the temperature of 36-38 ℃, the pressure of 0.1kPa, the stirring speed of 10r/min and the pH value of 7.0 to obtain a liquid traditional Chinese medicine fermentation mixture, and performing spray drying at the temperature of 65 ℃ to obtain fermentation mixture powder; wherein the adding proportion of the mixed powder and the fermentation medium is 8 g: 1L;
s3: and subpackaging the solid fermentation mixture and warehousing.
Wherein the formula of the fermentation medium in the S2 is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5g of glucose, 1 g of soluble starch, 5g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water, mixing, adjusting the pH value to 6-6.5 by using 10% sodium hydroxide solution, and sterilizing at 121 ℃ for 15min under high pressure.
Wherein the probiotics inoculated in the S2 is a secondary seed liquid of saccharomyces cerevisiae and bacillus licheniformis strains, the volume ratio of the secondary seed liquid to the fermentation culture medium is 8 vol%, and the preparation steps of the secondary seed liquid are as follows:
(1) activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 12-24 h at 36-38 ℃ to obtain a primary seed solution; wherein the addition ratio of Saccharomyces cerevisiae in seed culture medium is 5 × 108cfu/ml, Bacillus licheniformis 5X 108cfu/ml；
(2) Inoculating the first-stage seed liquid into a seed culture medium according to the inoculation amount of 10 vol%, and statically culturing for 12hh at 36-38 ℃ to obtain a second-stage seed liquid.
Wherein the formula of the seed culture medium for preparing the first-stage seed liquid and the second-stage seed liquid is as follows: 10 g of refined peptone, 5g of yeast extract, 20g of glucose, 5g of sodium acetate, 2 g of ammonium citrate, 0.1 g of tween 80, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
Example 4
The embodiment provides a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites, which is prepared by the following preparation method:
s1: taking 32 parts of radix sophorae flavescentis extract, 28 parts of oriental wormwood extract, 32 parts of hairyvein agrimony extract, 32 parts of antifebrile dichroa extract, 28 parts of cyrtomium rhizome extract, 22 parts of betel nut extract, 22 parts of Chinese torreya seed extract, 18 parts of pumpkin seed extract and 22 parts of liquorice extract according to parts by weight to form mixed powder;
s2: uniformly mixing the mixed powder and a fermentation culture medium, inoculating probiotics, fermenting for 30 hours at the temperature of 36-38 ℃, the pressure of 0.07kPa, the stirring speed of 25/min and the pH value of 6.5 to obtain a liquid traditional Chinese medicine fermentation mixture, and performing spray drying at the temperature of 60 ℃ to obtain fermentation mixture powder; wherein the adding proportion of the mixed powder and the fermentation medium is 20 g: 1L;
s3: and subpackaging the solid fermentation mixture and warehousing.
Wherein the formula of the fermentation medium in the S2 is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5g of glucose, 1 g of soluble starch, 5g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water, mixing, adjusting the pH value to 6-6.5 by using 10% sodium hydroxide solution, and sterilizing at 121 ℃ for 15min under high pressure.
Wherein the probiotics inoculated in the S2 is a secondary seed liquid of saccharomyces cerevisiae and bacillus licheniformis strains, the volume ratio of the secondary seed liquid to the fermentation culture medium is 6 vol%, and the preparation steps of the secondary seed liquid are as follows:
(1) activating probiotic strains, inoculating the activated probiotic strains in a seed culture medium, and statically culturing at 36-38 ℃ for 12-24 h to obtain a first-stage seed solution, wherein saccharomyces cerevisiae is added in the seed culture mediumThe addition ratio of the seed culture medium is 5 × 108cfu/ml, Bacillus licheniformis 5X 108cfu/ml；
(2) Inoculating the first-stage seed solution into a seed culture medium according to the inoculation amount of 7 vol%, and performing static culture at 36-38 ℃ for 16h to obtain a second-stage seed solution.
Wherein the formula of the seed culture medium for preparing the first-stage seed liquid and the second-stage seed liquid is as follows: 10 g of refined peptone, 5g of yeast extract, 20g of glucose, 5g of sodium acetate, 2 g of ammonium citrate, 0.1 g of tween 80, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
Application example 1 application test for controlling ascaris suum and acarid
30 sows are stored in the cages 1862664 × of the old poplar in forest town of Heilongjiang province.
A pig farm of young poplars is characterized in that 200 weaned piglets (60-day old) are stored in a pen for 9 months in 2019. Because of small lean-out, 60 piglets suffering from ascaris are examined by a microscope, and 90 piglets suffering from acariasis are examined from piglets with rough hair. The 90 heads are taken as basic groups, and a roundworm control test and a mite control test are respectively carried out:
① test for controlling ascaris suum by fermented preparation
The weaned piglets with roundworms detected were divided into 3 groups of 60. Wherein: in 20 groups of experiment 1, a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is added into drinking water, and the drinking water is continuously used for 7 days according to the proportion of 100 g of the solid state fermentation traditional Chinese medicine additive to 1000 jin of drinking water; in 20 groups of experiments 2, a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is added into drinking water, and the drinking water is continuously used for 7 days according to the proportion of 100 g of the solid state fermentation traditional Chinese medicine additive to 1000 jin of drinking water; in 20 control groups, ivermectin is added into drinking water, and the dosage is required according to the product instruction.
TABLE 1 basic data of the application test of controlling ascaris lumbricoides with fermented traditional Chinese medicine additive
Control roundworm application test Start (60 days old) data
At the beginning of the test, the number of each group was consistent, and the body weight was consistent (the difference was not significant).
At the end of the 30-day-old test period, 90-day-old mean weight data were obtained as follows:
control roundworm application test 90-day-old (end-of-test) data
At the end of the test, no ascaris suum was detected in the test 1 group, the test 2 group and the control group, which indicates that: roundworm is effectively controlled whether in the test group or the control group. However, the test group grew faster than the control group (the difference was significant); the activity rate of the test composition is higher than that of the control group (the difference is obvious).
According to the rough external surface coat, the excrement of the 90-day-old framed pig is subjected to microscopic examination, and 60 ascarid pigs are detected. Dividing into three groups, adding a solid fermentation Chinese medicinal additive for preventing and controlling livestock and poultry parasite into drinking water, and continuously using for 7 days according to the proportion of 100 g of the product and 1000 jin of drinking water; in 20 groups of experiments 2, a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is added into drinking water, and the drinking water is continuously used for 7 days according to the proportion of 100 g of the solid state fermentation traditional Chinese medicine additive to 1000 jin of drinking water; in 20 control groups, ivermectin is added into drinking water, and the dosage is required according to the product instruction.
Application test data of 90-day-old framed pig for controlling roundworm
When the test pigs reach the age of 110 days, the skin of each group of the test pigs is bright and the hair is glossy. The secondary fecal examination result shows that: no ascarid was detected in each group, and 5 ascarids were still detected in the control group.
This test shows that: compared with ivermectin, the fermented traditional Chinese medicine additive for controlling ascaris suum has the advantages that although the ivermectin can control ascaris suum, the ivermectin has certain toxic and side effects on pigs, and the long-term speed of the pigs is limited.
② test for controlling pig acarid by Chinese medicinal fermented preparation
The mites-detected weaned pigs 60 were divided into 3 groups. Wherein: in 20 groups of experiment 1, a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is added into drinking water, and the drinking water is continuously used for 7 days according to the proportion of 100 g of the solid state fermentation traditional Chinese medicine additive to 1000 jin of drinking water; in 20 groups of experiments 2, a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is added into drinking water, and the drinking water is continuously used for 7 days according to the proportion of 100 g of the solid state fermentation traditional Chinese medicine additive to 1000 jin of drinking water; in 20 control groups, ivermectin is added into drinking water, and the dosage is required according to the product instruction.
Control of mites application test Start (60 days old) data
At the beginning of the test, the number of each group was consistent, and the body weight was consistent (the difference was not significant).
At the end of 30 days (test period), 90-day-old average weight data were obtained as follows:
control of mites Using test 90 days old (end of test) data
At the end of the test, no mites were detected on the body surfaces of the test 1 group, the test 2 group and the control group, which indicates that: the mites were effectively controlled in both the test and control groups. However, the test group showed a higher growth rate than the control group (significant difference).
According to the appearance inspection, 60 pigs (scratchy mutually, have a large amount of crust on the epidermis and have obvious shedding of the hair) with mites and 90-day-old skeleton pigs are divided into 3 groups. Wherein: in 20 groups of experiment 1, a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is added into drinking water, and the drinking water is continuously used for 7 days according to the proportion of 100 g of the solid state fermentation traditional Chinese medicine additive to 1000 jin of drinking water; in 20 groups of experiments 2, a solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is added into drinking water, and the drinking water is continuously used for 7 days according to the proportion of 100 g of the solid state fermentation traditional Chinese medicine additive to 1000 jin of drinking water; in 20 control groups, ivermectin is added into drinking water, and the dosage is required according to the product instruction.
Application test data of 90-day-old framed pig for controlling mites
When the test pigs reach the age of 110 days, the skin of each group of the test pigs is bright red, the fallen furs are completely recovered to grow before the test, and the scab skin is completely fallen. And the fur has luster. The microscopic examination result shows that: no longer mites were detected in each group, and 3 mites were detected in the control group.
This test shows that: the fermented traditional Chinese medicine additive for controlling pig mites has advantages over ivermectin, and although the ivermectin can control pig ascaris, the use of the ivermectin has certain toxic and side effects on pigs, so that the long-term speed of the pigs is limited.
Application example 2 control of Pantograph disease test
130 sows on stock fence of Shi nan Zhou you county Tang 1310033 × (X D.C.
The stock pig farm dies more than 5% of the pigs due to bow diseases every year. In order to prevent and control the occurrence of pig arches, sulfonamides are used for preventing every month for 5 days. However, if the sulfonamides are used for a long time, the potential safety hazard of the food is caused.
In 7 months in 2019, 240 pigs (105-day-old) were placed in shelves and divided into 3 groups of 80 pigs each. Wherein: test 1 group 80, adding a solid fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites into drinking water, and continuously using for 7 days according to the proportion of 100 g of the product and 1000 jin of drinking water, No. 1-7 per month; experiment 2, 80 groups, wherein 100 g of the product and 1000 jin of drinking water are added into drinking water, and the drinking water is 1-7 in each month for 7 days; in 80 control groups, sulfonamides are added into drinking water, and the dosage is required according to the product specification.
Control of pig Bow application test Start (105 days old) data
At the beginning of the test, the number of each group was consistent, and the body weight was consistent (the difference was not significant).
At the end of 60 days (test period), 165-day-old average weight data were obtained as follows:
control Bow body application test 165 days old (end of test) data
At the end of the test, no pig bow-shaped body is found on the body surfaces of the test 1 group, the test 2 group and the control group, which shows that: the control group can effectively control the pig toxoplasma body no matter in the test group or the control group. However, the test group showed a higher growth rate than the control group (significant difference).
This test shows that: the use of the fermented traditional Chinese medicine additive for controlling the pig bow has advantages over sulfonamides, and although the use of the sulfonamides can control the pig bow, the use of the sulfonamides has certain toxic and side effects on pigs, so that the growth speed of the pigs is limited.
Description of other embodiments
In addition to the tests of the examples 1 and 2, the tests of controlling the cestodes, hookworms, filarial pulmonary, sarcoptidae, ticks, lice, eperythrozoon, toxoplasma, plasmodium and the like by the traditional Chinese medicine additive for preventing and controlling the solid state fermentation of the parasites of the livestock and poultry are respectively carried out, and the expected effect is achieved. Are too long for space and are not described in detail, and can be contacted privately if needed.
In summary, the following steps: the traditional Chinese medicine microecological preparation provided by the invention is reasonable in component proportion, takes traditional Chinese medicines as fermentation raw materials, utilizes the optimized saccharomyces cerevisiae and bacillus licheniformis to carry out biotransformation on intermediate substances, and is prepared into a traditional Chinese medicine microecological preparation which is non-toxic, residue-free, green, easy to absorb and obviously enhanced in antibacterial activity, so that the drug effect is obviously improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. The solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites is characterized by being prepared by the following preparation method:
s1: taking 30-40 parts of radix sophorae flavescentis extract, 25-35 parts of oriental wormwood extract, 25-35 parts of hairyvein agrimonia herb and bud extract, 25-35 parts of antifebrile dichroa extract, 20-30 parts of cyrtomium rhizome extract, 20-30 parts of betel nut extract, 20-30 parts of Chinese torreya seed extract, 15-25 parts of pumpkin seed extract and 15-25 parts of liquorice extract according to parts by weight to form mixed powder;
s2: uniformly mixing the mixed powder with a fermentation culture medium, inoculating probiotics, fermenting at 36-38 ℃ for 24-48 h to obtain a liquid traditional Chinese medicine fermentation mixture, and performing spray drying at 50-65 ℃ to obtain fermentation mixture powder;
s3: and subpackaging the solid fermentation mixture and warehousing.
2. The solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites according to claim 1, which is characterized in that: the formula of the fermentation medium in the S2 is as follows: 10 g of peptone, 10 g of beef powder, 3 g of yeast powder, 5g of glucose, 1 g of soluble starch, 5g of sodium chloride, 3 g of sodium acetate, 0.5 g of L-cysteine hydrochloride, 0.5 g of agar and 1000ml of purified water, mixing, adjusting the pH value to 6-6.5 by using 10% sodium hydroxide solution, and sterilizing at 121 ℃ for 15min under high pressure.
3. The solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites according to claim 1, which is characterized in that: and the probiotics in the S2 are saccharomyces cerevisiae and bacillus licheniformis.
4. The solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites according to claim 3, which is characterized in that: the probiotics inoculated in the S2 are secondary seed liquid of probiotic strains, and the preparation steps of the secondary seed liquid are as follows:
(1) activating probiotic strains, inoculating the activated probiotic strains into a seed culture medium, and statically culturing for 12-24 h at 36-38 ℃ to obtain a primary seed solution;
(2) inoculating the primary seed solution into a seed culture medium according to the inoculation amount of 2-10 vol%, and performing static culture at 36-38 ℃ for 12-24 h to obtain a secondary seed solution.
5. The solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites according to claim 4, which is characterized in that: and inoculating the second-stage seed liquid in the S2 with the fermentation culture medium according to the volume ratio of 3 vol% -8 vol%.
6. The solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites according to claim 4, which is characterized in that: the formula of the seed culture medium for preparing the first-stage seed liquid and the second-stage seed liquid is as follows: 10 g of refined peptone, 5g of yeast extract, 20g of glucose, 5g of sodium acetate, 2 g of ammonium citrate, 0.1 g of tween 80, 0.58 g of magnesium sulfate, 0.28 g of manganese sulfate and 1000ml of distilled water.
7. The solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites according to claim 1, which is characterized in that: the pressure intensity in the S2 fermentation is 0.02 kPa-0.1 kPa, the stirring speed is 10 r/min-30 r/min, and the pH value is 6.0-7.0.
8. The solid state fermentation traditional Chinese medicine additive for preventing and controlling livestock and poultry parasites according to claim 1, which is characterized in that: the addition ratio of the mixed powder to the fermentation medium in the S2 is (8-20) g: 1L of the compound.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112076274A (en) * | 2020-08-13 | 2020-12-15 | 广东施特威生物科技有限公司 | Environment-friendly pesticide prepared by traditional Chinese medicine fermentation technology |
| CN112618620A (en) * | 2020-07-20 | 2021-04-09 | 六安恒佳生物科技有限公司 | Solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls and preparation method thereof |
| CN112617046A (en) * | 2020-07-20 | 2021-04-09 | 六安恒佳生物科技有限公司 | Solid-state fermentation traditional Chinese medicine additive for laying fowls and preparation method thereof |
| CN115192642A (en) * | 2022-07-05 | 2022-10-18 | 江苏三仪生物工程有限公司 | Preparation method and application of composite biological preparation with parasite killing function and containing brevibacillus laterosporus and fermented traditional Chinese medicine |
-
2019
- 2019-12-25 CN CN201911371348.0A patent/CN111110772A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112618620A (en) * | 2020-07-20 | 2021-04-09 | 六安恒佳生物科技有限公司 | Solid-state fermentation traditional Chinese medicine additive for controlling respiratory diseases of laying fowls and preparation method thereof |
| CN112617046A (en) * | 2020-07-20 | 2021-04-09 | 六安恒佳生物科技有限公司 | Solid-state fermentation traditional Chinese medicine additive for laying fowls and preparation method thereof |
| CN112076274A (en) * | 2020-08-13 | 2020-12-15 | 广东施特威生物科技有限公司 | Environment-friendly pesticide prepared by traditional Chinese medicine fermentation technology |
| CN115192642A (en) * | 2022-07-05 | 2022-10-18 | 江苏三仪生物工程有限公司 | Preparation method and application of composite biological preparation with parasite killing function and containing brevibacillus laterosporus and fermented traditional Chinese medicine |
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