CN108653422B

CN108653422B - Chinese herbal medicine preparation for treating piglet diarrhea and preparation method thereof

Info

Publication number: CN108653422B
Application number: CN201810425463.0A
Authority: CN
Inventors: 颜健华; 刘升军; 赵德丰; 熊毅
Original assignee: Xing'an Animal Disease Prevention And Control Center; Guangxi University
Current assignee: Guangxi Nanning Shenjiu Biological Products Co ltd
Priority date: 2018-05-07
Filing date: 2018-05-07
Publication date: 2021-03-23
Anticipated expiration: 2038-05-07
Also published as: CN108653422A

Abstract

The invention discloses a Chinese herbal medicine preparation for treating piglet diarrhea and a preparation method thereof, belonging to the field of animal medicine. The Chinese herbal medicine preparation consists of 25-40 parts of sanguisorba root, 20-35 parts of abacus leaf, 0.5-2.0 parts of shikimic acid powder and 15-30 parts of edible sugar. The preparation method comprises decocting radix Sangusorbae and fructus Glochidionis Purpureae twice, mixing decoctions, adding shikimic acid powder and edible sugar, mixing, and dissolving completely. The invention has reasonable formula, simple preparation method, safety, no toxic or side effect, no drug residue, no drug resistance and high bioavailability, and has obvious curative effect on viral and bacterial piglet diarrhea.

Description

Chinese herbal medicine preparation for treating piglet diarrhea and preparation method thereof

Technical Field

The invention relates to the field of animal medicine, in particular to a Chinese herbal medicine preparation for treating piglet diarrhea and a preparation method thereof.

Background

The diarrhea of piglets becomes an important factor influencing the survival rate of piglets, and particularly, once the piglets which are just born suffer from diarrhea, the death rate is up to 60-90 percent, thus seriously influencing the economic benefit of the pig industry in China. The diarrhea of piglets mainly comprises viral diarrhea and bacterial diarrhea, and the viral diarrhea is mainly caused by that viruses invade intestinal villus cells to cause cell death to influence the absorption function of the intestines. At present, viruses that have been shown to cause diarrhea in piglets are: transmissible gastroenteritis virus, rotavirus, porcine epidemic diarrhea virus, porcine circovirus, porcine enterovirus, kubambusvirus, bocavirus, and the like. The transmissible gastroenteritis virus and the epidemic diarrhea virus are susceptible to pigs of various ages and breeds, wherein the fatality rate of piglets below 2 weeks can reach 100%; rotavirus is susceptible to infection of piglets of 10-60 days old, and is mainly characterized by anorexia, diarrhea, emesis, watery or pasty feces, and serious patients are dehydrated and even die; the bocavirus is a virus newly found in 2009, and is researched to cause host diarrhea and has correlation with the diarrhea epidemic situation of 2010 pigs. The bacterial diarrhea is mainly caused by the pollution of pathogenic bacteria and the change of environment and climate, and has serious harm to escherichia coli, salmonella, clostridium welchii, shigella and the like, particularly, yellow and white scour of piglets caused by the escherichia coli has great influence on pig farms, and the mortality rate is high. The single virus, bacteria or mixed infection can indirectly or directly cause diarrhea of piglets, once the piglets have diarrhea, the piglets can stop growing, even grow backwards, and the serious piglets die, thus causing great economic loss to the pig industry.

Viral diarrhea occurs almost in all countries of the world where pigs are raised. At present, no medicine for effectively treating the viral diarrhea of piglets exists, and effective methods for preventing the disease are to strengthen the feeding management and effective vaccination. However, no specific and ideal vaccine control measures for the viral diarrhea of piglets exist at present. The inactivated vaccine has low antigenicity and needs to be added with an adjuvant additionally. Various antiviral drugs such as ribavirin and amantadine have been prohibited by Ministry of agriculture. The treatment of bacterial diarrhea has insignificant treatment effect and difficult control of yellow and white diarrhea in a pig farm because pathogenic bacteria easily generate drug resistance to antibiotics.

The Chinese herbal medicine is the most traditional and naturally existing medicinal material in China, has extremely rich resource sources and relatively low price, contains various natural chemical components and nutrient components, such as tannin, saponin, alkaloid, organic acid, vitamins, mineral substances, trace elements and the like, has the effects of resisting bacteria, viruses, inflammation and convergence, can improve the immune function, improve the production performance, supplement nutrition and the like, and does not have the defect that western medicine antibiotics generate drug resistance, so the Chinese herbal medicine has wide application value in the prevention and treatment of livestock and poultry diseases. Under the condition that most of current swine plagues are mixed and infected frequently, the traditional Chinese medicine without adverse reaction is used for treating and controlling invasion and spread of pathogens, so that the traditional Chinese medicine has certain advantages, and the Chinese herbal medicine preparation is rarely reported to be used for treating or preventing diarrhea in swine herds.

Disclosure of Invention

The invention aims to solve the problems in the prior art and provides a Chinese herbal medicine preparation which has the advantages of reasonable formula, obvious curative effect, simple preparation method, adoption and operation of each basic unit, no drug resistance and no drug residue and can effectively treat piglet diarrhea.

The raw materials of the invention are all from Chinese herbal medicines, and the Chinese herbal medicine preparation for treating piglet diarrhea is scientifically prepared, has the effects of resisting bacteria, resisting virus, resisting inflammation, astringing, strengthening the spleen, warming the stomach, improving the immunity and the like, is suitable for piglet diarrhea caused by viral and bacterial factors, has no toxic or side effect, has no medicine residue, and can improve the immunity of the organism.

In order to realize the purpose of the invention, the adopted technical scheme is as follows:

a Chinese herbal medicine preparation for treating piglet diarrhea consists of the following components in parts by weight: 25-40 parts of sanguisorba root, 20-35 parts of abacus leaf, 0.5-2.0 parts of shikimic acid powder and 15-30 parts of edible sugar.

Preferably, the edible sugar is one or two of brown sugar and brown sugar.

Preferably, the composition consists of the following components in parts by weight: 25-35 parts of sanguisorba root, 25-30 parts of abacus leaf, 1.0-2.0 parts of shikimic acid powder and 20-25 parts of brown sugar.

Preferably, the composition consists of the following components in parts by weight: 30-40 parts of sanguisorba root, 20-25 parts of abacus leaf, 1.0-1.5 parts of shikimic acid powder and 15-25 parts of brown sugar.

Preferably, the composition consists of the following components in parts by weight: 30 parts of sanguisorba root, 25 parts of abacus leaf, 1 part of shikimic acid powder and 20 parts of yellow sugar.

Preferably, the composition consists of the following components in parts by weight: 25 parts of sanguisorba root, 30 parts of abacus leaf, 1.5 parts of shikimic acid powder and 25 parts of brown sugar.

Preferably, the Chinese herbal medicine preparation is a water agent.

The invention also provides a preparation method of the Chinese herbal medicine preparation for treating piglet diarrhea, which comprises the following steps:

(1) weighing the components according to the weight parts of the raw materials;

(2) putting radix Sangusorbae and folium Rubi into casserole, adding drinking water, soaking for 20-30 min, adding water to submerge the medicinal materials, decocting the casserole on furnace fire, boiling for 20-25 min, and pouring out the medicinal liquid to obtain first medicinal liquid;

(3) adding warm water into the casserole, decocting for the second time, boiling for 15-20 minutes, pouring out the liquid medicine, mixing with the first liquid medicine, naturally cooling, adding shikimic acid powder and brown sugar, and fully dissolving to obtain the Chinese herbal medicine preparation for treating piglet diarrhea.

The invention also provides a use method of the Chinese herbal medicine preparation for treating piglet diarrhea, and the Chinese herbal medicine preparation is taken for 3 days, wherein the Chinese herbal medicine preparation is 40 mL each time and 2 times a day.

The invention also provides application of the Chinese herbal medicine preparation for treating piglet diarrhea in preparation of medicines for treating or preventing piglet diarrhea.

(1) Root of sanguisorba officinalis. The garden burnet is also named as cucumber incense, sweet potato, pig ginseng and blood rocket grass; is dried root and rhizome of sanguisorba officinalis of Rosaceae. The plants are grown in mountainous slopes, forest edges, grasslands, meadows, bushes, field sides and the like, and are distributed in north China, northeast China, east China, southwest China, northwest China, Henan China, Hubei China, Hunan China, Guangxi China and the like. The main components are as follows: contains tannin, sanguisorbin and vitamin A. Nature and taste: bitter, sour and slightly cold. It enters liver, stomach and large intestine meridians. The pharmacological action is as follows: (i) convergence. Can stop bleeding and stop diarrhea in gastrointestinal tract, and relieve exudation when used for treating burn; ② antibiosis. Mainly has antibacterial effect on Escherichia coli, dysentery bacillus, Bacillus proteus, typhoid bacillus, paratyphoid bacillus, Pseudomonas aeruginosa, Vibrio cholerae, tubercle bacillus, meningococcus, etc.; ③ replenishing qi and nourishing blood, invigorating spleen and tonifying kidney. Can improve immunity of organism, can be used for treating leucopenia caused by various reasons such as virus and cell infection and some unknown reasons, and can also be used for treating thrombocytopenia, immunologic hypofunction, and aplastic anemia; fourthly, anti-inflammatory action. The water extract is injected into abdominal cavity of rat, can obviously inhibit inflammatory exudation caused by prostaglandin E1, and has certain anti-inflammatory effect. Has the effects of resisting bacteriophage, inactivating bacteriophage, and inhibiting bacteriophage reproduction in thallus, but not preventing bacteriophage from adsorbing bacteria.

(2) A glochidion pubescens (Thunb.) Ramsl. The Glochidion puberum Thunb is a plant of Euphorbiaceae. Erect multi-branch shrubs with the height of l-3 m. Branches are grayish brown, densely rusted or brownish yellow short and soft. Leaf intergrowth; the petiole is 1-3mm long and is molted; the leaf supporting triangle is 1-2mm long and is in a narrow triangle shape, and the quilt is molted; the leaves are round to long oval or needle-shaped, thin oval or inverted oval, 3-9cm long, 1.2-3.5cm wide, blunt to sharp tip at tip, thin and nearly round, and often have small tip, wedge-shaped base to blunt, upper part is only middle pulse is sparse short and soft or almost no hair, lower part is pink green, dense short and soft, 5-8 pairs of side pulse, and lower part is obvious. The flower is single, same or different, the flower is small, 2-5 flowers cluster in the leaf axil; no petal;

sepals

6, 2 rounds; the male flower has thin peduncle, length of l-8mm, is usually molted, the sepals are thick, are long round to long and narrow round or long round inverted egg-shaped, and are thinned and molted outside; 3 stamens are combined to form a columnar shape without degenerated ovaries; the stems of the female flowers are l-3mm long, thick, soft and hairy, the calyx is similar to that of the male flowers, but is slightly short and thick, and both surfaces of the calyx are hairy; the ovary is closely covered with villi, the chamber is 8-10, the flower column is combined to form a ring shape, the length and the width are equal to the length and the width of the ovary, the tip is not expanded, and the part connected with the ovary is contracted. Capsule is oblate, has diameter of 8-15mm, has 8-10 obvious longitudinal grooves, has annular slightly-elongated Huhua column at tip, is dense, short, soft, red at maturity, and has kidney-like shape, trigone, length of about 4mm, and reddish brown color. The flowering period is 6-10 months, and the fruit period is 8-12 months. Living in the hillside irrigation clusters. The distribution is Fujian, Guangdong, Guangxi, Guizhou, Sichuan, Hubei, Jiangxi, Zhejiang, Jiangsu, Anhui, Shaanxi, etc. The root and leaf are used as the medicine. Slightly bitter, astringent and cool; it enters kidney meridian because of its slight toxicity. Collecting root all year round, slicing and drying in the sun; collected in summer and autumn, and dried in the sun. The root, stem, leaf and fruit can be used as the medicine for relieving swelling, removing toxicity, treating dysentery and checking diarrhea. Has pharmacological action. The antibacterial effect is as follows: the leaf decoction has inhibiting effect on 9 kinds of intestinal bacteria such as shigella dysenteriae, paratyphoid A and salmonella typhi, wherein the intestinal bacteria is most sensitive to shigella dysenteriae, and the titer in a test tube is 1: 1280; the root decoction has no antibacterial effect.

(3) (ii) shikimic acid. Shikimic acid is a monomer compound extracted from Chinese medicine star anise, is present in dried mature fruit of magnolia star anise, and is white fine powder and easily soluble in water. Shikimic acid has antiinflammatory and analgesic effects, and can be used as intermediate of antiviral and anticancer drugs. Is mainly used for preventing and treating viral diseases such as avian influenza, infectious bronchitis, infectious laryngotracheitis, newcastle disease, infectious bursal disease and the like. The star anise belongs to a variety with homology of medicine and food specified by the Ministry of health, is a food and a medicine, and has no toxic or side effect.

(4) Brown sugar. The brown sugar is generally sugarcane finished sugar with honey, and generally refers to the honey sugar formed by juicing sugarcane, simply processing and concentrating. It retains more nutrient components of sugarcane, is easier to be digested and absorbed by human body, can quickly supplement physical strength and increase vitality, so that it is also called "oriental chocolate", in which it not only contains carbohydrate capable of providing heat energy, but also contains riboflavin, carotene, nicotinic acid and various elements of trace elements of manganese, zinc and chromium, etc. In addition, the calcium content of the brown sugar is 10 times of that of the white sugar, and the iron content of the brown sugar is 3.6 times of that of the white sugar. Brown sugar has effects of invigorating spleen, replenishing qi, invigorating spleen and stomach, and warming stomach.

(5) Yellow sugar. The brown sugar is prepared from sugar canes containing about 95% of cane sugar, and is prepared by cutting and rolling harvested sugar canes, removing impurities such as soil, bacteria and fibers from pressed juice, then decocting the juice for 5-6 hours with slow fire, continuously stirring the juice to evaporate water slowly, so that the concentration of sugar is gradually increased, and high-concentration syrup can be solidified into solid massive coarse sugar after being cooled, namely a brown sugar brick. The brown sugar has high sweetness, contains rich substances, and has the effects of replenishing blood, nourishing liver, relieving pain, invigorating spleen, warming stomach, dispelling cold and removing blood stasis.

The invention has the beneficial effects that:

1. the raw materials of the invention are Chinese herbal medicines which are 'medicinal and edible', and the invention has the advantages of safety, no toxic or side effect, no pollution, no drug residue, no drug resistance, high bioavailability and obvious curative effect. Overcomes the defects that the Chinese herbal medicine for treating piglet diarrhea in the current market has overlarge grain diameter, slow medicine release, low bioavailability, poor clinical effect and the like due to unreasonable formula or simple crushing.

2. The formula is reasonable, the preparation method is simple, the product quality is stable, the antiviral and antibacterial effects are strong, the functions of diminishing inflammation, astringing and improving the immunity of the organism are achieved, and the effect of treating the piglet diarrhea is obvious. Meanwhile, the palatability is good, and the operation is simple when the mask is used.

3. Clinical application proves that the traditional Chinese medicine composition has obvious curative effect on treating piglet diarrhea, can greatly reduce the toxic amount of viruses and bacteria, has no medicine residue, ensures that pork products meet the pollution-free requirement, and effectively ensures the health of people.

Drawings

FIG. 1 shows the PCR detection results of the epidemic diarrhea virus in the pig farm in example 6; wherein 1: positive control, 2-8 are different pig farm pathological materials, 9: negative control, M: DNA Standard DL 2000.

FIG. 2 shows the results of PCR detection of transmissible gastroenteritis in swine materials of example 6; wherein 1: positive control, 2-4 are respectively pig farm pathological material, 5: negative control, M: and (5) Maker.

Detailed Description

The invention will now be described in further detail with reference to the following examples, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The experimental methods used in the following examples are all conventional methods unless otherwise specified, and materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Example 1 preparation of a Chinese herbal medicinal preparation for treating diarrhea in piglets

A Chinese herbal medicine preparation for treating piglet diarrhea is prepared by the following steps:

(1) weighing the following components in parts by weight: 300 g of sanguisorba root, 250 g of abacus leaf, 10 g of shikimic acid powder and 200 g of yellow sugar;

(2) putting 300 g of sanguisorba roots and 250 g of abacus leaves into a marmite, adding 500 mL of cool drinking water, soaking for 20-30 minutes, adding water to submerge the medicine, putting the medicine marmite on a furnace fire, decocting with medium fire, boiling for 20 minutes, pouring out 300 mL of liquid medicine, then adding a proper amount of warm water, decocting for a second time, decocting for 15 minutes, pouring out the liquid medicine, mixing with the first time, cooling, adding 10 g of shikimic acid powder and 200 g of yellow sugar, and fully dissolving to obtain the medicine for filling.

Example 2

(1) weighing the following components in parts by weight: 250 g of sanguisorba root, 300 g of abacus leaf, 15 g of shikimic acid powder and 250 g of brown sugar;

(2) putting 250 g of sanguisorba roots and 300 g of abacus leaves into a marmite, adding 500 mL of cool drinking water, soaking for 20-30 minutes, adding water to submerge the medicine, putting the medicine marmite on a furnace fire, decocting with medium fire, boiling for 20 minutes, pouring out 300 mL of liquid medicine, then adding a proper amount of warm water, decocting for a second time, decocting for 15 minutes, pouring out the liquid medicine, mixing with the first time, cooling, adding 15 g of shikimic acid powder and 250 g of brown sugar, and fully dissolving to obtain the medicine for filling.

Example 3

(1) weighing the following components in parts by weight: 350 g of sanguisorba root, 300 g of abacus leaf, 20 g of shikimic acid powder and 150 g of brown sugar;

(2) putting 350 g of sanguisorba roots and 300 g of abacus leaves into a marmite, adding 600 mL of cool drinking water, soaking for 20-30 minutes, adding water to submerge the medicine, putting the medicine marmite on a furnace fire, decocting with medium fire, boiling for 20 minutes, pouring out 300 mL of liquid medicine, then adding a proper amount of warm water, decocting for a second time, decocting for 15 minutes, pouring out the liquid medicine, mixing with the first time, cooling, adding 20 g of shikimic acid powder and 100 g of brown sugar, and fully dissolving to obtain the medicine for filling.

Example 4

(1) weighing the following components in parts by weight: 300 g of sanguisorba root, 200 g of abacus leaf, 15 g of shikimic acid powder and 250 g of brown sugar;

(2) putting 300 g of sanguisorba roots and 200 g of abacus leaves into a marmite, adding 500 mL of cool drinking water, soaking for 20-30 minutes, adding water to submerge the medicine, putting the medicine marmite on a furnace fire, decocting with medium fire, boiling for 20 minutes, pouring out 300 mL of liquid medicine, then adding a proper amount of warm water, decocting for a second time, decocting for 15 minutes, pouring out the liquid medicine, mixing with the first time, cooling, adding 15 g of shikimic acid powder and 250 g of brown sugar, and fully dissolving to obtain the medicine for filling.

Examples of the applications

Example 5

The Chinese herbal medicine preparation prepared in the example 1 is applied to diarrhea piglets, and the using steps and the effects are as follows:

1. case selection criteria

(1) Piglets of 0-60 days old

(2) The symptoms are manifested by mental depression, anorexia, recumbency, emesis, yellow or pasty feces discharge, dehydration, etc.

(3) Piglet diarrhea caused by bacterial factors was confirmed by bacterial isolation in a veterinary laboratory. Aseptically taking mesenteric lymph nodes of sick pigs, inoculating the mesenteric lymph nodes to a nutrient agar plate, taking bacterial colonies to inoculate a Macconk agar plate, picking out red bacterial colonies with medium size, smooth bulges and roundness from the Macconk agar plate, and performing a serum factor test, wherein the Escherichia coli 0 antigen reaction is positive and is identified as Escherichia coli. The medium-sized, smooth, convex and round colorless bacterial colony is picked out from the Macconkey agar plate, and the Salmonella is identified as the positive result of the antigen reaction of the Salmonella 0 in the serum factor test. Meanwhile, the mesenteric lymph node sample is subjected to PCR detection of virus, and the virus is negative.

2. Criteria for therapeutic effect

And (3) curing: the food has no symptoms of emesis, diarrhea, dehydration, etc., and has normal intestinal digestion and good mental state.

The method has the following advantages: the frequency of vomit and diarrhea is reduced, dehydration symptom is relieved, and the mental state is improved.

And (4) invalidation: the clinical symptoms worsen and even die.

3. Treatment regimens

The number of 502 pigs was determined from 8 different pig farms (farmers) meeting the above case criteria, of which 6 were responsible for E.coli and 2 were responsible for Salmonella. The application method comprises drenching, and taking 40 mL/head each time, 2 times daily, and continuously taking for 3 days.

4. Therapeutic results

After 3 days of administration, the affected piglets were subjected to clinical examination, and the results are shown in Table 1.

TABLE 1 clinical treatment results for bacterial diarrhea

Group of	Recovery method	Invalidation
			Salmonella choleraesuis	91.8（56/61）	8.2%(5/61)
Colibacillosis	95.9%(423/441)	4.1%(18/441)

As can be seen from the table 1, when the Chinese herbal medicine preparation disclosed in the embodiment 1 of the invention is used for treating the diarrhea piglets, the cure rate of the sick piglets is high, and the effect is obvious, so that the Chinese herbal medicine preparation disclosed by the invention can effectively control the morbidity of the diarrhea of the piglets aiming at the bacterial diarrhea of the piglets, the piglets are good after being cured, and no side effect is caused.

Example 6

1. case selection criteria

(1) Piglets of 0-60 days old

(3) The diarrhea of piglets caused by viral factors is confirmed by RT-PCR method in veterinary laboratories (figure 1, figure 2). Diagnosis quoted Guangxi local standards: the RT-PCR method for detecting Porcine Epidemic Diarrhea Virus (PEDV) (DB 45/T1012-2014) and the RT-PCR method for detecting porcine transmissible gastroenteritis virus (DB 45/T1007-2014).

2. Criteria for therapeutic effect

And (4) invalidation: the clinical symptoms worsen and even die.

3. Treatment regimens

986 pigs are collected from 14 different pig farms (farmers) meeting the above case standard, wherein 12 pigs (households) and 2 pigs (households) with infectious gastrointestinal property are caused by the epidemic diarrhea virus. The application method comprises drenching, and taking 40 mL/head each time, 2 times daily, and continuously taking for 4 days.

4. Therapeutic results

After taking the medicine for 4 days, clinical examination and virus detection are carried out on the sick piglets. The results are shown in Table 2.

TABLE 2 clinical treatment results for viral diarrhea

Group of	Recovery method	Invalidation	PCR detection result of feces
				Transmissible gastroenteritis	91.1%（112/123）	8.9%(11/123)	Negative of
Epidemic diarrhea virus disease	88.9%(767/863)	11.1%(96/863)	Negative of

As can be seen from the table 2, when the Chinese herbal medicine preparation disclosed in the embodiment 1 of the invention is used for treating the diarrhea piglets, the cure rate of the sick piglets is high, and the effect is obvious, so that the Chinese herbal medicine preparation disclosed by the invention can effectively control the morbidity of the diarrhea of the piglets aiming at the viral diarrhea of the piglets, the piglets are good after being cured, and no side effect is caused.

Example 7 safety test:

50 healthy piglets of 20 days old are selected and divided into 5 groups, each group has 10 piglets, the Chinese herbal medicine preparation prepared by the embodiment 1 of the invention is used for being irrigated for 7 days, and a group of blank control groups (healthy piglets which are not administrated) is arranged. During the dosing period, the condition of 5 groups of piglets was observed. The results show that the piglets of the drug group and the control group have no adverse or abnormal performance, the food intake and the water intake are normal, and the weight gain is not obviously different from that of the blank control group. The traditional Chinese medicine composition is safe, has no toxic or side effect, and has no adverse effect on the growth of piglets.

The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the inventive work should be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.

Claims

1. The Chinese herbal medicine preparation for treating piglet diarrhea is characterized by being prepared from the following components in parts by weight: 30 parts of sanguisorba root, 25 parts of abacus leaf, 1 part of shikimic acid powder and 20 parts of yellow sugar; the preparation method of the Chinese herbal medicine preparation comprises the following steps:

(1) weighing the components according to the weight parts of the raw materials;

(3) adding warm water into the casserole, decocting for the second time, boiling for 15-20 minutes, pouring out the liquid medicine, mixing with the first liquid medicine, naturally cooling, adding shikimic acid powder and yellow sugar, and fully dissolving to obtain the Chinese herbal medicine preparation for treating piglet diarrhea.

2. The Chinese herbal preparation for treating piglet diarrhea according to claim 1, wherein the Chinese herbal preparation is aqua.

3. The use of a herbal formulation according to claim 1 for the treatment of diarrhea in piglets in the manufacture of a medicament for the treatment or prevention of diarrhea in piglets.