CN102657712A - Preparation method for adonis coerulea maxim extract and application thereof - Google Patents
Preparation method for adonis coerulea maxim extract and application thereof Download PDFInfo
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- CN102657712A CN102657712A CN2012101767774A CN201210176777A CN102657712A CN 102657712 A CN102657712 A CN 102657712A CN 2012101767774 A CN2012101767774 A CN 2012101767774A CN 201210176777 A CN201210176777 A CN 201210176777A CN 102657712 A CN102657712 A CN 102657712A
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- small cup
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Abstract
The invention discloses a preparation method for adonis coerulea maxim extract and application thereof. The preparation method for the adonis coerulea maxim extract includes: crushing crude dried adonis coerulea maxim, soaking with solvent fully to obtain extract, and concentrating the extract to obtain finished extract. The used extraction solvent is any of water, methanol, ethanol, isopropanol, ether, trichloromethane, ethyl acetate, petroleum ether or acetone, or mixed solutions mixed by some of the solvents in optional proportions. The adonis coerulea maxim extract can be used for preparing medicaments for killing mites or preparing medicaments for killing mites outside animal bodies.
Description
Technical field
The present invention relates to the purposes of a kind of natural plants extractum method for preparing and this extractum.The present invention relates to blue flower side gold small cup extract making method exactly, and the purposes of blue flower side gold small cup extractum.
Background technology
Blue flower side gold small cup (Adonis coerulea Maxim.) belongs to Ranunculaceae Adonis herbaceos perennial, high 3～15cm; Mainly be distributed in alpine meadow or the shrubbery of China Tibet, Sichuan, Qinghai and Gansu height above sea level 3350～5200m, for being applied to treat skin infection and psoriasic medical material in the Tibetan medicine.Fitochemical studies shows that it mainly contains compositions such as orientin, side gold small cup alcohol, Lutonaretin, luteolin, apigenin, daucosterol.But the rarely seen report of biological activity about indigo plant flower side gold small cup.
The animal acaricide is acarid zooparasite body surface and the chronic epizootic disease of a kind of animal that causes; Its cause of disease is acaricide, itch mite; Mainly betide autumn end, winter and the early spring,, extensively betide animals such as cattle, sheep, horse, dog, rabbit, chicken through infected animal and the direct or indirect contact infection of healthy animal; Also can infect to the people, in the pastoral area, farming and pastoral area and large-scale cultivation field infection rate and sickness rate be all higher.Appetite depression or useless takes place should disease mainly to show as and cut off, becomes thin, mao come off etc. in animal, influences fertility performance and the economic worth of animal, seriously restricts animal husbandry sound development and economic benefit.At present; The medicine that is used to treat the animal acaricide is mainly chemosynthesis and antibiotics, and is clean etc. like ivermectin, demodicid mite, and this type of medicine is in effective control animal acaricide; Because drug residue has caused problems such as public health, food safety and ecological environment again.
Summary of the invention
The present invention provides the blue flower of natural plants side gold small cup extract making method, and a kind of purposes of blue flower side gold small cup extractum is provided simultaneously.
Blue flower side gold small cup extractum method for preparing of the present invention is; With the blue flower of drying side gold small cup crude drug; Pulverize, obtain extracting solution after fully soaking with solvent, obtain total extractum through concentrating again; Used extractant is any in water or methanol or ethanol or isopropyl alcohol or ether or chloroform or ethyl acetate or petroleum ether or the acetone, or appoints several kinds of mixed solutions that constitute with arbitrary proportion in the aforementioned solvents.
The preferred blue flower side gold small cup extractum method for preparing of the present invention is to extract with ethanol or methanol, when obtaining more effective ingredient, has guaranteed the curative effect of medicine.
Indigo plant flower side gold small cup extractum with the inventive method preparation can be used for preparing the medicine of killing acarid, perhaps is used to prepare the medicine of killing the outer acarid of animal body.
The invention has the advantages that:
1. blue flower side gold small cup be a natural plants, and its extract has safety, effective, low, the free of contamination characteristics of toxicity aspect treatment animal acaricide.
2. blue flower side gold its plantation of small cup and processing are very little to environmental effect, and medicine is noresidue in use, has guaranteed the safety of animal derived food.
3. the used indigo plant of the present invention is spent side gold small cup abundant raw material, and extraction process is simple, and cost is low, can conveniently be prepared into various preparations.
The specific embodiment
One, blue flower side gold small cup extractum extracts embodiment
Take by weighing dry blue flower side gold small cup, pulverize, spend the night with 75% soak with ethanol, hot bath refluxed 1～5 hour, extract 1～4 time, and merge extractive liquid,, sucking filtration, the Rotary Evaporators evaporation and concentration, freezing lyophilizing, ultraviolet radiation sterilization obtains total extractum, and yield is 22.5%.
Or with after the pulverizing of indigo plant flower side gold small cup; With in methanol, ethanol, isopropyl alcohol, ether, chloroform, ethyl acetate, petroleum ether, the acetone any, or appoint several kinds of mixed solutions that constitute with arbitrary proportion in the water, methanol, ethanol, isopropyl alcohol, ether, chloroform, ethyl acetate, petroleum ether, acetone.Extract with aforementioned solution or mixed solution, again extract is concentrated, preparation extractum.
Two, blue flower side gold small cup extractum is killed the embodiment of rabbit acarid
1. experimental animal and itch mite
Psoroptes cuniculi (Psoroptes cuniculi) is collected in the rabbit of artificial challenge's psoroptic acariasis, the rabbit of natural infection itch mite, and body weight 1.8～2.2kg, Lanzhou Institute of Biological Products's Experimental Animal Center provides.Collect crust from the external auditory meatus of rabbit severe infections, place glass dish, then plate is placed 0.5h at 35 ℃, go out itch mite in the microscopically isolation identification, to choose the energetic itch mite of worm pin collection subsequent use with softish.
2. test drug
Blue flower side gold small cup extractum is the previous embodiment preparation, before use, uses the dilution of 10% glycerol to be 500mg/ml, 250mg/ml, 125mg/ml and 62.5mg/ml. extractum
3. test method
The 100 μ l indigo plant of four kinds of dosage is spent in adding 24 orifice plates of side gold small cup extract, added filter paper and draw unnecessary liquid.With choosing the worm pin 12 itch mites being chosen in each hole successively, is blank with the mixture of 10% glycerol, and each concentration is established 5 repetitions.Then 24 orifice plates are put into test environment (25 ℃, 75% relative humidity) and cultivate, every separated 3h with microscopic examination once observed 24 hours, write down the death condition of each period.When the polypide podomere long-time motionless or be judged to be death when reactionless with needle stimulus.
4. result
The result shows that blue flower side gold small cup extract has stronger external insecticidal action.High dose (500mg/ml) all kills itch mite at In vitro culture in 18 hours, and the dosage of 250mg/ml killed whole itch mites in 24 hours.See table 1 for details.
The external psoroptes cuniculi that kills of the blue flower of table 1 side gold small cup extract is active
Three, blue flower side gold small cup extractum is used to kill the zoopery of the outer acarid of animal body
1. experimental animal
The rabbit of natural infection acarid, body weight 1.8～2.2kg, Lanzhou Institute of Biological Products's Experimental Animal Center provides.
2. test drug
Blue flower side gold small cup extractum is that previous embodiment is extracted preparation.Before the use, extractum water and glycerol dilution are 250mg/ml.
3. test method
20 rabbits that infect acarid are divided into two groups at random, and first group whenever sprayed medicine 2ml at a distance from three days to rabbit external auditory meatus crust place, sprays altogether three times.Second group as the blank group, whenever at a distance from three days to rabbit external auditory meatus crust place's spray water and glycerol 2ml, spray three times.Observed altogether 15 days for above-mentioned two groups.
4. curative effect determinate standard
Recovery from illness: rabbit external auditory meatus is not seen crust, scrapes the skin that takes a morsel and examines under a microscope and do not see polypide.
Take a turn for the better: rabbit external auditory meatus crust obviously reduces, and scrapes to get crust and examine under a microscope polypide quantity and reduce.
Invalid: rabbit external auditory meatus crust is not seen minimizing, examines under a microscope polypide quantity and does not see minimizing.
5 results
Blue flower side gold small cup extract sprays treatment 10 examples, 5 examples of fully recovering, and 4 examples that take a turn for the better, invalid 1 example, cure rate is 50%, total effective rate is 90%.The blank group is not seen recovery from illness and is taken a turn for the better.Can find out that from the result blue flower side gold small cup extract for treating rabbit acaricide has good effect.See table 2 for details.
The effect of the blue flower of table 2 side gold small cup extract for treating rabbit acaricide
Claims (4)
1. blue flower side gold small cup extractum method for preparing; It is characterized in that exsiccant blue flower side gold small cup crude drug; Pulverize, obtain extracting solution after fully soaking with solvent, obtain total extractum through concentrating again; In used extractant available water or methanol or ethanol or isopropyl alcohol or ether or chloroform or ethyl acetate or petroleum ether or the acetone any, or several kinds of mixed solutions that constitute with arbitrary proportion in the aforementioned solvents.
2. the described blue flower side gold small cup extractum method for preparing of claim 1 is characterized in that used solvent is ethanol or methanol.
3. the indigo plant flower side gold small cup extractum of claim 1 or 2 methods preparation is used to prepare the medicine of killing acarid.
4. the indigo plant flower side gold small cup extractum of claim 1 or 2 methods preparation is used to prepare the medicine of killing the outer acarid of animal body.
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| CN2012101767774A CN102657712A (en) | 2012-05-30 | 2012-05-30 | Preparation method for adonis coerulea maxim extract and application thereof |
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| CN2012101767774A CN102657712A (en) | 2012-05-30 | 2012-05-30 | Preparation method for adonis coerulea maxim extract and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103385927A (en) * | 2013-07-16 | 2013-11-13 | 中国农业科学院兰州畜牧与兽药研究所 | Traditional Chinese medicine composition for the treatment of animal acariasis |
| CN106344668A (en) * | 2016-08-30 | 2017-01-25 | 青海民族大学 | Effective parts of skyblue adonis and preparation method and application thereof |
| CN107158211A (en) * | 2017-06-29 | 2017-09-15 | 阿保地 | A kind of desinsection Chinese medicine composition and preparation method thereof |
| WO2021017407A1 (en) * | 2019-07-30 | 2021-02-04 | 刘纯久 | Polysaccharide and use thereof |
| CN114907920A (en) * | 2022-06-27 | 2022-08-16 | 两面针（江苏）实业有限公司 | Mite-removing laundry detergent rich in various plant extracts |
| CN114907920B (en) * | 2022-06-27 | 2024-05-28 | 两面针（江苏）实业有限公司 | Mite-removing laundry detergent rich in various plant extracts |
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| CN101069702A (en) * | 2007-06-08 | 2007-11-14 | 西北农林科技大学 | Efficient, low-toxic and environment-protective plant insect-killing extract |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103385927A (en) * | 2013-07-16 | 2013-11-13 | 中国农业科学院兰州畜牧与兽药研究所 | Traditional Chinese medicine composition for the treatment of animal acariasis |
| CN106344668A (en) * | 2016-08-30 | 2017-01-25 | 青海民族大学 | Effective parts of skyblue adonis and preparation method and application thereof |
| CN107158211A (en) * | 2017-06-29 | 2017-09-15 | 阿保地 | A kind of desinsection Chinese medicine composition and preparation method thereof |
| CN107158211B (en) * | 2017-06-29 | 2020-08-11 | 阿保地 | Traditional Chinese medicine composition for killing ticks and preparation method thereof |
| WO2021017407A1 (en) * | 2019-07-30 | 2021-02-04 | 刘纯久 | Polysaccharide and use thereof |
| CN114907920A (en) * | 2022-06-27 | 2022-08-16 | 两面针（江苏）实业有限公司 | Mite-removing laundry detergent rich in various plant extracts |
| CN114907920B (en) * | 2022-06-27 | 2024-05-28 | 两面针（江苏）实业有限公司 | Mite-removing laundry detergent rich in various plant extracts |
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Application publication date: 20120912 |