CN105287692A - Dalbergia benthami prain extractive, and preparation method and application thereof - Google Patents
Dalbergia benthami prain extractive, and preparation method and application thereof Download PDFInfo
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- CN105287692A CN105287692A CN201510833439.7A CN201510833439A CN105287692A CN 105287692 A CN105287692 A CN 105287692A CN 201510833439 A CN201510833439 A CN 201510833439A CN 105287692 A CN105287692 A CN 105287692A
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Abstract
The invention discloses a dalbergia benthami prain extractive, which is obtained from the stem of dalbergia benthami prain through reflux extraction with ethyl acetate. The invention discloses a preparation method of the dalbergia benthami prain extractive. The preparation method comprises: pulverizing the stem of dalbergia benthami prain to obtain power; performing reflux extraction of the powder with ethyl acetate at 80 DEG C for 2-4 times, wherein each reflux extraction lasts for 2-3h; combining the obtained filtrate from reflux extraction to obtain an extracting solution; removing ethyl acetate from the extracting solution; and performing drying to obtain the dalbergia benthami prain extractive. The invention discloses an application of the dalbergia benthami prain extractive to preparation of anti-inflammatory and antibacterial medicines. The dalbergia benthami prain extractive has an obvious effect on anti-inflammation and antibiosis, and can replace other medicines, liable to sensitize, to be applied to anti-inflammation and antibiosis, thereby providing a novel approach.
Description
Technical field
The present invention relates to a kind of Lignum Dalbergiae benthamii extract and its preparation method and application.
Background technology
Inflammation (inflammation) refers to the biological tissue with vascular system, to the pathological process based on defense reaction that various pro-inflammatory cytokine causes damage to occur.Basic pathology be changed to rotten, ooze out and hypertrophy.
Many Chinese herbal medicine, crude drug have suppression killing action to antibacterial, and its antiinflammatory action comes into one's own day by day.Strong medicine Lignum Dalbergiae benthamii DalbergiabenthamiPrain, have another name called: any of several broadleaf plants rattan fiber crops, two Guangdong wingceltises, liana, in being born in sparse woods and bushes, be distributed in Guangxi, Guangdong, the ground such as Hainan, medicinal part is stem, have another name called rattan spring CaulisDalbergiabenthami, can be used for restoring menstrual flow and invigorating blood circulation, cure mainly traumatic injury, the symptoms such as bones and muscles pain and caused by energy stagnation and blood stasis menoxenia, rheumatism and external use medicament for traumatic injury is mainly used among the people, but still scientific basis is lacked to the exploitation of its pharmacologically active, greatly have impact on exploitation and the application clinically of Lignum Dalbergiae benthamii, not yet have at present about Lignum Dalbergiae benthamii extract antiinflammatory and antibacterial on application note.
Summary of the invention
An object of the present invention is to solve at least the problems referred to above, and the advantage will illustrated at least is below provided.
A further object of the invention is to provide a kind of Lignum Dalbergiae benthamii extract, and dry acquisition after extracting its stem by ethyl acetate backflow, Lignum Dalbergiae benthamii extract of the present invention has obvious antiinflammatory and antibacterial action.
In order to realize, according to these objects of the present invention and other advantage, providing a kind of Lignum Dalbergiae benthamii extract, it is the extract that the stem adopting ethyl acetate backflow to extract Lignum Dalbergiae benthamii obtains.
A kind of preparation method of Lignum Dalbergiae benthamii extract, Lignum Dalbergiae benthamii is got its stem and pulverize to obtain powder, by described powder ethyl acetate reflux, extract, 2-4 time at 80 DEG C, each 2-3h, the filtrate merging reflux, extract, obtains extracting solution, remove the ethyl acetate in described extracting solution, after drying, namely obtain Lignum Dalbergiae benthamii extract.
Preferably, the preparation method of described Lignum Dalbergiae benthamii extract, extracts 3 times by described powder ethyl acetate backflow, each 2.5h.
Preferably, the preparation method of described Lignum Dalbergiae benthamii extract, described drying is vacuum drying, and baking temperature is lower than 5 DEG C.
Preferably, the preparation method of described Lignum Dalbergiae benthamii extract, during each reflux, extract, the consumption of ethyl acetate is 5-10 times of thing weight to be extracted.
An application for Lignum Dalbergiae benthamii extract, described Lignum Dalbergiae benthamii extract is preparing the application in antiinflammatory and antibacterial pharmaceutical preparation.
Preferably, the application of described Lignum Dalbergiae benthamii extract, described pharmaceutical preparation includes Lignum Dalbergiae benthamii extract and the pharmaceutically acceptable adjuvant composition of effective amount.
Preferably, the application of described Lignum Dalbergiae benthamii extract, described pharmaceutical preparation is oil preparation, ointment, ointment, paste or plaster.
The present invention at least comprises following beneficial effect: Lignum Dalbergiae benthamii method for preparing extractive is simple, obtain easily, for antiinflammatory with antibacterial there is remarkable effect, antiinflammatory is strengthened and antibacterial effect has wide market sight to being applied in other products, the application on medicine of penicillin and berberine can be replaced, be applicable to the user to penicillin or berberine allergy, thus make up consumer and antiinflammatory and antibacterial some drugs cannot be used to provide a new way because of irritated.
Part is embodied by explanation below by other advantage of the present invention, target and feature, part also will by research and practice of the present invention by those skilled in the art is understood.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, can implement according to this with reference to description word to make those skilled in the art.
It should be noted that experimental technique described in following embodiment if no special instructions, is conventional method, described reagent and material, if no special instructions, all can obtain from commercial channels.
Raw material sources: Lignum Dalbergiae benthamii, through being accredited as pulse family Dalbergia plant (DalbergiabenthamiPrain).
A kind of Lignum Dalbergiae benthamii extract is the extract that the stem adopting ethyl acetate backflow to extract Lignum Dalbergiae benthamii obtains.
Embodiment 1:
A kind of preparation method of Lignum Dalbergiae benthamii extract, Lignum Dalbergiae benthamii is got its stem, naturally dry, pulverize to obtain powder, by described powder ethyl acetate reflux, extract, 2 times at 80 DEG C, each 2h, the filtrate merging reflux, extract, obtains extracting solution, removes the ethyl acetate in described extracting solution, namely obtains Lignum Dalbergiae benthamii extract after drying, wherein, each consumption extracting the ethyl acetate used is 5 times of thing weight to be extracted.
Embodiment 2:
A kind of preparation method of Lignum Dalbergiae benthamii extract, Lignum Dalbergiae benthamii is got its stem, naturally dry, pulverize to obtain powder, by described powder ethyl acetate reflux, extract, 3 times at 80 DEG C, each 2.5h, the filtrate merging reflux, extract, obtains extracting solution, remove the ethyl acetate in described extracting solution, after drying, namely obtain Lignum Dalbergiae benthamii extract.Wherein, each consumption extracting the ethyl acetate used is 8 times of thing weight to be extracted.
Embodiment 3:
A kind of preparation method of Lignum Dalbergiae benthamii extract, Lignum Dalbergiae benthamii is got its stem, naturally dry, pulverize to obtain powder, by described powder ethyl acetate reflux, extract, 4 times at 80 DEG C, each 3h, the filtrate merging reflux, extract, obtains extracting solution, remove the ethyl acetate in described extracting solution, after drying, namely obtain Lignum Dalbergiae benthamii extract.Wherein, each consumption extracting the ethyl acetate used is 10 times of thing weight to be extracted.
Embodiment 4:
Lignum Dalbergiae benthamii stem is dried in the shade, 50g is taken after pulverizing, reflux 3 times with at 400mL ethyl acetate 80 DEG C, each 3h, merges three filtrates, filtrate is put distilling under reduced pressure in Rotary Evaporators, recycling design, residue low-temperature vacuum drying, to dry, obtains Lignum Dalbergiae benthamii stem position ethyl acetate extract (EAE), i.e. Lignum Dalbergiae benthamii extract of the present invention, yield is generally 2.0%-2.8%.
Embodiment 5:
The Lignum Dalbergiae benthamii extract of embodiment 4 was pulverized 200 mesh sieves, get appropriate oil phase substrate, consistency modifiers and emulsifying agent and prepare oil phase at about 75 DEG C heating and meltings, get the mixing of appropriate wetting agent, stabilizing agent and water to be placed in container 70-75 DEG C of heating, aqueous phase is obtained after mixing, after oil phase is mixed with water, ultrasonic mixing, adds Lignum Dalbergiae benthamii extract at 50-60 DEG C, while stir, cool and obtain the preparation of the different dosage forms such as oil preparation, ointment, ointment, paste or plaster.Concrete dosage form is based on the product needed be applicable to, wherein in general every milliliters of liquid preparation or every gram of mastic preparation Lignum Dalbergiae benthamii extractive content about 20-200mg, the content of Lignum Dalbergiae benthamii extract is controlled according to the type difference of appropriate products, such as face uses, then should follow a small amount of principle repeatedly.
Wherein, oil phase substrate includes but not limited to stearic acid, paraffin, Cera Flava, higher alcohol etc., and consistency modifiers includes but not limited to vaseline, liquid paraffin, plant wet goods, and emulsifying agent includes but not limited to the derivant of soap class emulsifying agent, polyoxyethylene ether; Wetting agent includes but not limited to glycerol, sorbitol, propylene glycol etc.; Stabilizing agent can preferentially use but be not limited to Calcium Disodium Versenate.
In order to further illustrate Lignum Dalbergiae benthamii extract of the present invention antiinflammatory and antibacterial on effect, the present invention by setting up the acute inflammation model of mice auricular concha swelling from the external anti-inflammatory activity evaluating Lignum Dalbergiae benthamii extract first, and adopts disc diffusion method and test tube method (MIC) antibacterial activity to Lignum Dalbergiae benthamii extract to be studied.
1. effective extract extracorporeal anti-inflammatory Effect study
(1) mice caused by dimethylbenzene xylene ear swelling test
Standard body weight kunming mice 40 is divided into four groups (blank groups, dosage in heavy dose of, the Lignum Dalbergiae benthamii of Lignum Dalbergiae benthamii, Lignum Dalbergiae benthamii low dose of) at random.On the left of mice, before and after auricular concha, uniform application caused by dimethylbenzene xylene in two sides is scorching, and 0.04ml/ only.After modeling success, be coated with respectively at the left ear inflammation part of mice, mouse right ear is left intact.After 4 hours, animal is put to death in dislocation, ears are cut immediately along auricle baseline, same area auricle is got with diameter 8mm card punch, ten thousand/analytical balance is weighed, subtracting left auricle weight by auris dextra sheet weight is swelling, scorching side auricle weight will be caused deduct contrast side auricle weight and be swelling, observe swelling change before and after medication.
Ear weight after ear swelling degree=coating-coating vestibule weight
(2) date processing: SPSS13.0 homogeneity test of variance.
Table 1, for the Lignum Dalbergiae benthamii extract recorded after 4h after coating is on the impact of mice ear, can be found out, along with Lignum Dalbergiae benthamii extract doses increases, press down swollen rate significantly to increase, illustrate that the antiphlogistic effects of Lignum Dalbergiae benthamii extract of the present invention is remarkable, adhere to use 3 times on the 1st, antiphlogistic effects is better.
Table 1 Lignum Dalbergiae benthamii extract is on the impact of mice ear
2. Lignum Dalbergiae benthamii extract antibacterial activity in vitro research
(1) bacterium solution preparation: by staphylococcus aureus, escherichia coli, bacillus pyocyaneus, bacillus subtilis, Bacillus typhi five kinds of strains respectively at sectional streak on MH agar plate, cultivates 24h for 37 DEG C; The single colony inoculation of each strain of inoculating loop difference picking, in MH meat soup, cultivates 24h in 37 DEG C; Adjust the concentration of five kinds of bacterium by Maxwell turbidimetry, ultimate density is 1.5 × 106CFU/mol.
(2) disc diffusion method: the aseptic spreading rod of bacteria suspension getting 0.1ml is spread evenly across plating medium (about 15mL), take out after the filter paper dick of diameter 9mm after sterilizing is soaked 20min in medicinal liquid (concentration 250 μ g/mL), equidistantly be affixed on flat board, every ware puts 4, and every strain makees 3 plates.Take sterilized water as blank, penicillin is the positive control of gram-positive bacterium, and berberine is as the positive control of gram negative bacteria.Lignum Dalbergiae benthamii extract to the fungistatic effect of five kinds of bacterium in table 2.
Table 2 Lignum Dalbergiae benthamii extract is to the fungistatic effect of five kinds of bacterium
Note: "----" represent undetermined.
As shown in Table 2, Lignum Dalbergiae benthamii extract has obvious inhibition to bacillus subtilis, and inhibition zone size is 12mm, has obvious inhibition to staphylococcus aureus, and inhibition zone size is 13mm.
(3) test tube method (MIC): as table 3, measure and adopt test tube doubling dilution to carry out minimal inhibitory concentration mensuration, get sterile test tube 9,1st pipe adds pastille meat soup 1mL as negative control, drug level is 400mg/mL, add each 1mL of meat soup containing medicinal liquid in 2-8 pipe, its drug concentration makes two times of gradient dilutions successively, the 9th pipe add not containing the meat soup of medicinal liquid as positive control; Then 1-8 pipe respectively add 50ul cultivate 8h for examination bacterium liquid, after fully shaking up, cultivate 24h in 37 DEG C, observed result.Here confession examination bacterium liquid is the bacterium liquid of staphylococcus aureus and bacillus subtilis, and wherein, the concentration of these two kinds of bacterium is 1.5 × 106CFU/mol.
Table 3 minimal inhibitory concentration determination test
For above-mentioned experiment, applicant of the present invention show that the minimal inhibitory concentration of Lignum Dalbergiae benthamii extract to bacillus subtilis, staphylococcus aureus is respectively 100 and 50mg/mL.
In order to further illustrate the curative effect of Lignum Dalbergiae benthamii extract of the present invention, enumerate some cases:
Model case:
1) Mr. Zhang, 50 years old, rheumatic arthritis, redness and swelling of joints, pain on foot, smear and adopt the medicinal external emulsifiable paste prepared of Lignum Dalbergiae benthamii extract of the present invention (wherein, in every 1g medicinal external emulsifiable paste, the content of Lignum Dalbergiae benthamii extract is 50mg), 3 times on the 1st, use 7 days, red and swollen symptom weakens, adhere to use again 7 days, pain relief on foot, access after 2 months, pain obviously alleviates, adhere to using medicinal external emulsifiable paste of the present invention after 8 months, pay a return visit again, walk substantially without difference, be as good as with ordinary person, adhere to that use is after 12 months, pain disappears, adhere to that use is paid a return visit after 18 months again, the state of an illness there is no repeatedly.
2) simple Ms, 30 years old, use certain cosmetics to cause facial anaphylaxis, general red, inflammation, play macule, the oil preparation using Lignum Dalbergiae benthamii extract of the present invention to prepare is smeared (wherein, in every 1mL oil preparation, the content of Lignum Dalbergiae benthamii extract is 20mg), every day 3 times, use allergic symptom after 3 days to relax, obviously alleviate, continue use after 4 days, macule disappears, and inflammation is decorporated.
Although embodiment of the present invention are open as above, but it is not restricted to listed in description and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the general concept that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment described.
Claims (8)
1. a Lignum Dalbergiae benthamii extract, is characterized in that, is the extract that the stem adopting ethyl acetate backflow to extract Lignum Dalbergiae benthamii obtains.
2. the preparation method of a Lignum Dalbergiae benthamii extract as claimed in claim 1, it is characterized in that, Lignum Dalbergiae benthamii is got its stem and pulverize to obtain powder, by described powder ethyl acetate reflux, extract, 2-4 time at 80 DEG C, each 2-3h, the filtrate merging reflux, extract, obtains extracting solution, removes the ethyl acetate in described extracting solution, namely obtains Lignum Dalbergiae benthamii extract after drying.
3. the preparation method of Lignum Dalbergiae benthamii extract as claimed in claim 2, is characterized in that, described powder ethyl acetate backflow is extracted 3 times, each 2.5h.
4. the preparation method of Lignum Dalbergiae benthamii extract as claimed in claim 2, it is characterized in that, described drying is vacuum drying, and baking temperature is lower than 5 DEG C.
5. the preparation method of Lignum Dalbergiae benthamii extract as claimed in claim 2, is characterized in that, during each reflux, extract, the consumption of ethyl acetate is 5-10 times of thing weight to be extracted.
6. Lignum Dalbergiae benthamii extract as claimed in claim 1 and the application as the Lignum Dalbergiae benthamii extract in claim 2-5 prepared by any one, is characterized in that, described Lignum Dalbergiae benthamii extract is preparing the application in antiinflammatory and antibacterial pharmaceutical preparation.
7. the application of Lignum Dalbergiae benthamii extract as claimed in claim 6, is characterized in that, described pharmaceutical preparation includes Lignum Dalbergiae benthamii extract and the pharmaceutically acceptable adjuvant composition of effective amount.
8. the application of Lignum Dalbergiae benthamii extract as claimed in claim 7, it is characterized in that, described pharmaceutical preparation is oil preparation, ointment, ointment, paste or plaster.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107033115A (en) * | 2017-03-14 | 2017-08-11 | 广西中医药大学 | Lignum Dalbergiae benthamii ketone and its extracting method |
| CN107439426A (en) * | 2017-08-23 | 2017-12-08 | 何亮 | A kind of cultural method of loach |
| CN107466920A (en) * | 2017-08-23 | 2017-12-15 | 何亮 | A kind of cultural method of ecological loach |
| KR101847479B1 (en) * | 2017-06-14 | 2018-04-12 | 한국한의학연구원 | Composition for prevention or treatment of trychophytia comprising Dalbergia odorifera extract |
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| CN104341430A (en) * | 2014-09-30 | 2015-02-11 | 广西中医药大学 | 3-phenylcoumarin robustic acid as well as extraction method and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107033115A (en) * | 2017-03-14 | 2017-08-11 | 广西中医药大学 | Lignum Dalbergiae benthamii ketone and its extracting method |
| CN107033115B (en) * | 2017-03-14 | 2019-02-15 | 广西中医药大学 | Lignum Dalbergiae benthamii ketone and its extracting method |
| KR101847479B1 (en) * | 2017-06-14 | 2018-04-12 | 한국한의학연구원 | Composition for prevention or treatment of trychophytia comprising Dalbergia odorifera extract |
| CN107439426A (en) * | 2017-08-23 | 2017-12-08 | 何亮 | A kind of cultural method of loach |
| CN107466920A (en) * | 2017-08-23 | 2017-12-15 | 何亮 | A kind of cultural method of ecological loach |
| CN107466920B (en) * | 2017-08-23 | 2021-01-15 | 何亮 | Ecological loach breeding method |
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