CN107158211B - Traditional Chinese medicine composition for killing ticks and preparation method thereof - Google Patents
Traditional Chinese medicine composition for killing ticks and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/8965—Asparagus, e.g. garden asparagus or asparagus fern
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/534—Mentha (mint)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/44—Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an insecticidal traditional Chinese medicine composition, wherein the active pharmaceutical raw materials of the composition comprise asparagus officinalis, adonis adoxoides and crab shells or comprise mint, adonis adoxoides and crab shells, turpentine is taken as a transdermal agent, the total mortality of the composition in 24 hours for killing ticks in a laboratory is 95.0-96.0%, and the insect-repelling rates of sheep and cattle on the body surface in 3 days in a field test field are respectively 92.3-94.3% and 96.6-96.9%, so that the composition can be used as a traditional Chinese medicine composition for replacing chemical tick-repelling.
Description
Technical Field
The invention belongs to the field of veterinary medicines, and particularly relates to a traditional Chinese medicine composition for killing parasites, in particular to a traditional Chinese medicine composition for killing ectoparasite ticks and a preparation method thereof.
Background
Animal ectoparasitosis is a medical insect which causes parasitic insects to absorb animal blood on the body surface of an animal to transmit various animal and human diseases. The incidence of diseases in the Qinghai and Tibetan areas of animals raised in a grazing mode is high, and after the animals suffer from the diseases, the ingestion is reduced, the weight is reduced, the production performance and the breeding cost are increased, the animals die individually, and the economic benefits of animal husbandry production and breeders are influenced.
Ticks absorb animal blood on the body surface of animals to transmit various medical insects with animal and human diseases. The incidence of diseases in the south Tibetan region of the Qinghai and the western firewood wood basin for raising animals in a grazing mode is high, after the animals suffer from various epidemic diseases transmitted by ticks, the ingestion is reduced, the weight is reduced, the production performance and the raising cost are increased, the animals can die due to individual epidemic diseases, and the economic benefits of animal husbandry production and breeders are seriously influenced.
Currently, the drugs for expelling ticks are mainly chemical synthetic drugs such as ivermectin, abamectin, deltamethrin, chlorfenapyr and the like, and when the animals are treated with the epidemic diseases, chemical residues in the animal bodies and animal excreta containing the chemical drugs pollute the environment, and accompanying public health, animal-derived food safety and the like have many problems.
The natural plant Adonis coerulea maxim (Adonis coerulea maxim) (Tibetan: Doudor) produced in the Qinghai-Tibet plateau area is slightly toxic, and the whole dry grass is used as the medicine; root tuber of crab caraway (rhizome of Phlomis Younghshand Mukerigee) (Tibetan: Romor) and aerial whole herb of short leaf asparagus (Tibetan: nice) produced in the Tibet plateau, herba Menthae (Mentha haplocalyx Briq), and oleum Terebinthinae (transdermal agent). The natural traditional Chinese medicine and the natural Tibetan medicine have little influence on meat food safety and environmental pollution, and are medicinal materials for creating safe meat food products, green and organic foods and causing no chemical pollution to the environment in the future.
The application of the medicinal materials in killing ticks is not reported, and the application of the medicinal materials in killing ticks in combination is not seen.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides an insecticidal traditional Chinese medicine composition, wherein the active medicine raw materials of the traditional Chinese medicine composition comprise a first active medicine, a second active medicine and a third active medicine, wherein:
the first active drug is asparagus or mint, the second active drug is adonis sinica, and the third active drug is crab shell.
In some embodiments, the first active agent is whole grass.
In some embodiments, the second active agent is whole grass.
In some embodiments, the third active agent is administered using tuberous roots.
In some embodiments, the method of preparing the traditional Chinese medicine composition comprises:
chopping the first active drug and the second active drug, mashing the third active drug, mixing, and soaking in water to form a liquid medicine;
filtering the liquid medicine, taking the filtrate and adding turpentine oil to form the traditional Chinese medicine composition.
In some embodiments, the method of preparing the traditional Chinese medicine composition comprises:
chopping 20-100 parts by weight of the first active medicament and 20-100 parts by weight of the second active medicament, mashing 20-100 parts by weight of the third active medicament, mixing, and soaking with 200-1000 parts by weight of water to form a liquid medicament;
filtering the liquid medicine, taking the filtrate, and adding 1-50 parts of turpentine oil into the filtrate to form the traditional Chinese medicine composition.
In some embodiments, the method of preparing the traditional Chinese medicine composition comprises:
chopping 70 parts by weight of the first active drug and 70 parts by weight of the second active drug, mashing 70 parts by weight of the third active drug, mixing, and soaking with 600 parts by weight of water for 24 hours to form a liquid medicine;
filtering the liquid medicine, taking the filtrate, and adding 3 parts of turpentine oil into the filtrate to form the traditional Chinese medicine composition.
And are not limited to the specific values recited above, as such may be modified in accordance with the principles and practices of pharmaceutical formulation. Transdermal agents other than turpentine may be used instead of or in addition to transdermal agents. Other dosage forms and processing modes can be adopted based on the three traditional Chinese medicinal materials, such as decoction in water, preparation of tablets, pills and the like. The parts of the medicinal materials can be adjusted reasonably, and the technical characteristics can be adjusted and/or combined within a reasonable range.
The invention also provides application of any one of the traditional Chinese medicine compositions in preparing an insecticide.
The invention also provides application of any one of the traditional Chinese medicine compositions in preparing a tick-killing agent.
The invention also provides application of any one of the traditional Chinese medicine compositions in prevention and treatment of animal epidemic diseases.
In some embodiments of the composition, the total mortality rate of killing ticks in a laboratory for 24 hours is 95.0-96.0%, and the insect-repellent rates of sheep and cattle on the field test site for 3 days reach 92.3-94.3% and 96.6-96.9% respectively, so that the composition can be used as a traditional Chinese medicine composition for replacing chemical tick-killing. Therefore, the traditional Chinese medicine composition has application prospect.
Detailed Description
In order to better explain the technical solution of the present invention, the following detailed description is given of the embodiments of the present invention. The following examples are intended to further illustrate the invention but should not be construed as being limitations or restrictive thereon. Unless otherwise specified, technical features used in the embodiments may be replaced with other technical features known in the art having equivalent or similar functions or effects without departing from the inventive concept.
1 materials and methods
1.1 test drugs and materials
The Adonis blue flower, Eriocheir sinensis, and Asparagus parviflora are collected from Yushu city, mountain slope around Guermu city, ditch and grass cluster, herba Menthae, and oleum Terebinthinae are purchased from Chinese medicinal shop, and the water is tap water or spring water.
1.2 test animals tick
The test area is Yushu city of Tibet plateau in south of Qinghai and 2 Guermu city of West foot of Chadamu basin in west of Qinghai. Collecting ticks on the lower side of the neck, under the armpit, under the abdomen, etc. of the cattle and sheep; the cayenne tick is collected from the surface of sheep and cattle, and the gelmu tick is collected from the surface of sheep and cattle.
1.3 preparation of Single-ingredient and composition liquid medicine
1.3.1 short-leaf Asparagus extract
Weighing dry asparagus 70g without roots, cutting, soaking in 200g water for 24h, filtering the liquid medicine with fine gauze, taking the filtrate, and adding 1mL of turpentine to form the asparagus extract.
1.3.2 Adonis Lanceolata leach liquor
Weighing 70g of dry Adonis blue flower whole herb, mashing, soaking in 200g of water for 24h, filtering the liquid medicine with fine gauze, and adding 1mL of turpentine into the filtrate to form Adonis blue flower leachate.
1.3.3 crab shell leachate
Weighing 70g of dry crab shell root tuber, mashing, soaking in 200g of water for 24h, filtering the liquid medicine with fine gauze, and adding 1mL of turpentine oil into the filtrate to form crab shell leachate.
1.3.4 peppermint extract
Weighing 70g of dried and root-removed mint, cutting up, soaking in 200g of water for 24h, filtering the liquid medicine with fine gauze, and adding 1mL of turpentine into the filtrate to form mint leachate.
1.3.5 composition 1
Weighing 140g of chopped total asparagus officinalis, 140g of ground crab root tuber and 140g of adonis sinica, chopping 140g of adonis sinica, soaking in 1200mL of water for 24h, filtering with fine gauze, and adding 6mL of turpentine into the filtrate to obtain the composition.
1.3.6 composition 2
Weighing 140g of dried and root-removed mint, mincing 140g of crab shell root tuber, mincing 140g of blue flower adonis, soaking in 1200mL of water for 24h, filtering the liquid medicine with fine gauze, and adding 6mL of turpentine oil into the filtrate to form the composition.
2 laboratory tick killing experiment of single medicine, composition and control
Tick 50 from cows and sheep in the cajuput and gelmu sheep areas was used as an experimental unit and placed in a jar for future use.
Respectively sucking 50 ticks with 5ml dose of non-fat cotton by using short asparagus officinalis leachate, mint leachate, adonis bluebeard leachate, crab shell leachate, composition 1, composition 2 and normal water (control), putting the ticks into 3 jars for observation at intervals of 8h for 24h, recording the death condition of the ticks at each time period, and determining the ticks to die when the ticks are immobile or stir non-response by using a pick-up needle. The results of the experiments are shown in tables 1-5, where table 5 combines the data for mortality of ticks from different sources and is a summary of the data in tables 1-4.
TABLE 1 statistics of individual drugs and combinations of cajuput and sheep for killing ticks in laboratory
TABLE 2 Lab statistics of tick-killing of single-component medicines and compositions of German sheep
TABLE 3 statistics of pesticide for single drug and composite of cajaput and cattle in laboratory
TABLE 4 statistics of tick-killing of single-component and composite gelmu cattle in laboratory
TABLE 5 statistical summary of sheep and cattle single-herb and composite tick-killing statistics table in laboratory
3 field test of single medicine and composition for killing tick
Taking cattle and sheep infected with ticks in Yushu and gelmu areas, manually counting the tick load number on the surface of the sheep and cattle, spraying each group of liquid medicine to the surface of the animal body by using a sprayer for 1 time/day in the morning and evening, continuously spraying for 3 days, spraying 8mL of liquid medicine per square decimeter of skin, spraying normal water to a control group, and observing; and (4) tying and raising the ticks during spraying for 3 days, feeding forage, not grazing, and counting the number of the ticks killed in 3 days in total. The results of the experiments are summarized in tables 6-9, table 10 is a summary of the data in tables 6-7, and table 11 is a summary of the data in tables 8-9.
TABLE 6 cumulative field tick repelling and killing experiment for Jade tree sheep in vitro for 3 days with single and combined medicines
TABLE 7 cumulative field tick repelling and killing experiment for in vitro 3 days for single-medicine and combined-medicine Gelru sheep
TABLE 8 cumulative field tick-expelling and killing experiment for Jade tree cattle in vitro for 3 days with single and combined medicines
TABLE 9 cumulative field tick repelling and killing experiment for 3 days in vitro for single and combined medicine gelmu cattle
TABLE 10 cumulative field tick repelling and killing experiment for sheep with single and combined medicines for 3 days in vitro
TABLE 11 Total Table of cumulative field tick-expelling and killing experiments for 3 days for single and combined cattle
Discussion 4
For the composition 1, the mortality rate of the cajuput and gelmu 2 in the laboratory for 24 hours is 95.0 percent, and the insect-repellent rate of the body surfaces of sheep and cattle in the field test field for 3 days respectively reaches 92.3 percent and 96.9 percent.
For the composition 2, the mortality rate of the cajuput and gelmu 2 in the laboratory for 24 hours is 96.0 percent, and the insect-repellent rate of the body surfaces of sheep and cattle in the field test field for 3 days respectively reaches 94.3 percent and 96.6 percent.
The mortality of each group is very low under the control of normal water, which indicates that the ratio of natural death of the ticks is not high, the insecticidal effect of the two compositions is obviously higher than that of the single use of each component, and the three active ingredients of the medicines have mutual synergy.
Therefore, the two compositions have obvious overall effect, can be used as a novel harmless traditional Chinese medicine insect repellent for repelling and killing ticks of animals, have certain popularization value, and have certain economic significance for reducing the residue of chemical medicine insect repellent meat products, improving the physical health of people, improving the plateau environment and supporting the development of plateau pollution-free meat food.
The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (9)
1. A traditional Chinese medicine composition for killing ticks is characterized in that raw material medicines of the traditional Chinese medicine composition consist of a first active medicine, a second active medicine and a third active medicine, wherein:
the first active drug is asparagus or mint, the second active drug is adonis sinica, and the third active drug is crab shell;
the weight ratio range of the first active medicament, the second active medicament and the third active medicament is as follows: 1:(0.2-5):(0.2-5).
2. The herbal composition of claim 1, wherein said first active agent is whole grass.
3. The herbal composition of claim 1, wherein said second active agent is whole herb.
4. The traditional Chinese medicine composition for killing ticks according to claim 1, wherein the third active agent is root tubers.
5. The traditional Chinese medicine composition for killing ticks according to any one of claims 1-4, wherein the preparation method of the traditional Chinese medicine composition comprises the following steps:
chopping the first active drug and the second active drug, mashing the third active drug, mixing, and soaking in water to form a liquid medicine;
filtering the liquid medicine, taking the filtrate and adding turpentine oil to form the traditional Chinese medicine composition.
6. The traditional Chinese medicine composition for killing ticks according to claim 5, wherein the preparation method of the traditional Chinese medicine composition comprises the following steps:
chopping 20-100 parts by weight of the first active medicament and 20-100 parts by weight of the second active medicament, mashing 20-100 parts by weight of the third active medicament, mixing, and soaking with 200-1000 parts by weight of water to form a liquid medicament;
filtering the liquid medicine, taking the filtrate, and adding 1-50 parts of turpentine oil into the filtrate to form the traditional Chinese medicine composition.
7. The traditional Chinese medicine composition for killing ticks according to claim 6, wherein the preparation method of the traditional Chinese medicine composition comprises the following steps:
chopping 70 parts by weight of the first active drug and 70 parts by weight of the second active drug, mashing 70 parts by weight of the third active drug, mixing, and soaking with 600 parts by weight of water for 24 hours to form a liquid medicine;
filtering the liquid medicine, taking the filtrate, and adding 3 parts of turpentine oil into the filtrate to form the traditional Chinese medicine composition.
8. Use of a traditional Chinese medicine composition for killing ticks according to any one of claims 1-7 in the preparation of a tick-killing agent.
9. Use of the Chinese medicinal composition for killing ticks according to any one of claims 1-7 in the preparation of a medicament for controlling animal tick diseases.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102657712A (en) * | 2012-05-30 | 2012-09-12 | 中国农业科学院兰州畜牧与兽药研究所 | Preparation method for adonis coerulea maxim extract and application thereof |
| CN103385927A (en) * | 2013-07-16 | 2013-11-13 | 中国农业科学院兰州畜牧与兽药研究所 | Traditional Chinese medicine composition for the treatment of animal acariasis |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102657712A (en) * | 2012-05-30 | 2012-09-12 | 中国农业科学院兰州畜牧与兽药研究所 | Preparation method for adonis coerulea maxim extract and application thereof |
| CN103385927A (en) * | 2013-07-16 | 2013-11-13 | 中国农业科学院兰州畜牧与兽药研究所 | Traditional Chinese medicine composition for the treatment of animal acariasis |
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| Title |
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| 藏兽药蓝花侧金盏对兔螨的抑杀作用研究;尚小飞等;《中兽医医药杂志》;20121210(第06期);24-26 * |
| 藏药螃蟹甲的化学成分、药理活性及质量控制方法研究进展;栾飞等;《甘肃中医药大学学报》;20161025;第33卷(第05期);67-71 * |
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Inventor after: Wang Xiaorun Inventor after: Cai Dai Yang Inventor after: Shen Yanli Inventor after: Zhao Quanbang Inventor after: Hu Guangwei Inventor after: Li Jing Inventor after: Kan Wei Inventor after: Wang Rouchun Inventor after: Zeng Zhanhao Inventor after: Zahi Da Va Inventor after: Wang Haixiong Inventor after: Atami Inventor after: It's only time Inventor after: Zhou Yuhai Inventor after: Zhang Weizhong Inventor after: Yang Yiqing Inventor after: Zhang Zhiping Inventor after: Cai Jinshan Inventor after: Sang Ba Inventor after: Xiao Licheng Inventor after: Wang Yi Inventor before: Atami Inventor before: Cai Dai Yang Inventor before: Shen Yanli Inventor before: Zhao Quanbang Inventor before: Hu Guangwei Inventor before: Li Jing Inventor before: Kan Wei Inventor before: Wang Rouchun Inventor before: Zeng Zhanhao Inventor before: Zahi Da Va Inventor before: Wang Haixiong Inventor before: Zhang Weizhong Inventor before: It's only time Inventor before: Zhou Yuhai Inventor before: Wang Xiaorun Inventor before: Yang Yiqing Inventor before: Cai Jinshan Inventor before: Zhang Zhiping Inventor before: Sang Ba Inventor before: Xiao Licheng Inventor before: Wang Yi |
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