CN105961829A - Chinese herbal medicinal micro-ecological premixed feed and preparation method thereof - Google Patents
Chinese herbal medicinal micro-ecological premixed feed and preparation method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
- A23V2400/413—Acidilactici
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a Chinese herbal medicinal micro-ecological premixed feed. The Chinese herbal medicinal micro-ecological premixed feed comprises the Chinese herbal medicine raw materials and the microbial agents. The Chinese herbal medicine raw materials comprise honeysuckle flower, dandelion, radix astragali, bighead atractylodes rhizome, poria cocos, hyacinth bean, Chinese yam, hawthorn, cortex acanthopanacis, rhizoma atractylodis, oriental waterplantain rhizome, prepared fleece flower root, and gynostemma pentaphylla; and the microbial agents comprise streptococcus faecalis, bacillus subtilis, bacillus coagulans, pediococcus acidilactici, lactobacillus plantarum, microzyme, ferrous glycine chelate, and tryptophan. The Chinese herbal medicine raw materials and the microbial agents are mixed according to the proportion of 45-55% of the Chinese herbal medicine raw materials and 45-55% of the microbial agents. The invention also provides the preparation method of the Chinese herbal medicinal micro-ecological premixed feed. During the preparation processes of the Chinese herbal medicinal micro-ecological premixed feed, physiologically active substances having special effect for increasing livestock and poultry immunity are generated, sub-health level of the livestock and poultry in the large-scale culture fields can be improved, and the resistance capability of the livestock and poultry on invasion by harmful microbes can be increased.
Description
Technical field
The present invention relates to premixed feed, particularly relate to a kind of Chinese herbal medicine micro-ecological premixed feed and preparation method thereof, it is adaptable to poultry scale livestock farming.
Background technology
In recent years, along with improving constantly of people's living standard, the growth momentum of the livestock and poultry cultivation industry of China is gratifying, and continuous more than 20 years of main animal products yield is with double-digit speed increase.The main animal products yield of China all leaps into the front ranks of the world at present, and China has become as genuine livestock and poultry cultivation big country.All these all with livestock and poultry cultivation intensive, scale is inseparable.Although the intensive and large-scale development of livestock and poultry breeding industry meets people for meat, egg, the requirement of milk, and is made that bigger contribution for improving the living standard of urban and rural residents of China.But meanwhile the large-scale development of livestock and poultry cultivation also brings many problems.These problems include: 1, in livestock and poultry farms in scale; poultry are by high density, intensively raise; the quantity of motion of poultry was greatly reduced compared to former stocking model, and this makes poultry be in a kind of sub-health state all the time, mainly shows as dyspepsia, diarrhoea and constipation；2, poultry concentrated in a large number livestock and poultry cultivation house feed; air in poultry house is extremely stagnant; it is easy to grow harmful microorganism in poultry circle cage; in addition the poultry resistance that poultry are caused because being chronically at sub-health state significantly declines, and often breaks out multiple groupment livestock and poultry pestilence in livestock and poultry farms in scale.Once break out epidemic disease, the most then cultivation level significantly glides, heavy then poultry are the most dead, causes loss without redemption.
Livestock and poultry farms in scale solution to the problems described above the most directly uses veterinary drug treatment at present.The shortcoming of this method is that labor intensity is big, cost is high, and often can only alleviate the symptom of ill poultry to ill poultry are oral and drug administration by injection is treated to use veterinary drug treatment to refer to, it is impossible to the real immunity improving poultry.It addition, the veterinary drug used mostly is antibiotic, can not optionally eliminate harmful bacteria during treatment, often the most dominant probiotics in poultry body be killed together, reduce further the immunity of poultry.Additionally, use the method that harmful pathogenic microorganism can be caused to produce drug resistance, this can cause the dosage of poultry increasing, easily causes the antibiotic content in livestock meat to exceed standard, causes food-safety problem.
Summary of the invention
For solving one or more problems that existing livestock and poultry cultivation industry exists, it is an object of the invention to provide Chinese herbal medicine fermentable premixed feed of a kind of subhealth state level that can improve poultry in scale livestock farming and preparation method thereof.
For achieving the above object, the present invention provides a kind of Chinese herbal medicine micro-ecological premixed feed, including herbal raw material and microbial bacterial agent, herbal raw material includes Flos Lonicerae 5-15 part, Herba Taraxaci 5-15 part, Radix Astragali 5-15 part, Rhizoma Atractylodis Macrocephalae 3-7 part, Poria 5-15 part, Seem Lablab Album 3-7 part, Rhizoma Dioscoreae 3-7 part, Fructus Crataegi 5-10 part, Cortex Acanthopancis 5-10 part, Rhizoma Atractylodis 5-10 part, rushes down pool 10-15 part, Radix Polygoni Multiflori Preparata 4-8 part, Herb Gynostemmae Pentaphylli 3-7 part；Microbial bacterial agent includes streptococcus faecalis 10-20 part, bacillus subtilis 10-20 part, Bacillus coagulans 10-20 part, pediococcus acidilactici 5-15 part, Lactobacillus plantarum 10-20 part, yeast 10-20 part, ferrum glycinate 2-4 part, tryptophan 1-3 part；Described herbal raw material is mixed in following ratio with described microbial bacterial agent: herbal raw material 45%-55%, microbial bacterial agent 45%-55%.
Preferably, the formula of described herbal raw material is Flos Lonicerae 10 parts, Herba Taraxaci 10 parts, the Radix Astragali 10 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, 10 parts of Poria, 5 parts of Seem Lablab Album, Rhizoma Dioscoreae 5 parts, Fructus Crataegi 8 parts, Cortex Acanthopancis 7 parts, Rhizoma Atractylodis 7 parts, rushes down 12 parts of pool, Radix Polygoni Multiflori Preparata 6 parts, Herb Gynostemmae Pentaphylli 5 parts, and the formula of described microbial bacterial agent is streptococcus faecalis 15 parts, bacillus subtilis 15 parts, Bacillus coagulans 25 parts, pediococcus acidilactici 10 parts, Lactobacillus plantarum 15 parts, 15 parts of yeast, ferrum glycinate 3 parts, tryptophan 2 parts.
Further, also including that the mixture quality of maltose and Semen Maydis powder, herbal raw material and microbial bacterial agent is A, the quality of maltose is B, the quality of Semen Maydis powder is C, in mixture A: B: C=100: 0.5-1.5: 1-3)
Preferably, A: B: C=100: 1: 1.5.
The present invention also provides for the preparation method of a kind of said herbal medicine Tiny ecosystem premixed feed, comprises the following steps:
A. described herbal raw material is mixed with described microecological microbial agent；
B. by the mixture of said herbal medicine raw material and microecological microbial agent with maltose, Semen Maydis powder with 100: the ratio of 0.5-1.5: 1-3 mixes, and carries out preculture, and preculture temperature is 36-38 DEG C, and pre-incubation time is 60-75h；
C. fermenting, ferment at a temperature of 32-36 DEG C 75-96h.
Further, after step c, also include that tunning is spray-dried by step d., pulverize and discharge and be coated granulation.
Further, between step c and d, include the step e. detection microecological microbial agent viable count of tunning and content of physiologically active substance, detect qualified after terminate fermentation, if detecting defective, then continue fermentation.
The concrete examination criteria of above-mentioned tunning is: microbial bacterial agent viable count >=1.0 × 109CFU/g, in biological active substances, flavone compound >=2%, saponins compound >=4%, terpenoid >=3%, steroid compound >=2% in mass ratio.
The method have the advantages that (1) passes through pre-culture step, the quantity of microecological microbial agent is expanded (amplification times is 2-4 times), beneficially the microecological microbial agent bioconversion to herbal raw material；(2) by the microecological microbial agent after expanding propagation, part herbal raw material substrate (this part Chinese herbal medicine accounts for about the 65% of Chinese herbal medicine total amount) being carried out solid fermentation bioconversion, make to create in mixture has the biological active substances of certain effects (being respectively greater than or equal to 2%, 4%, 3% and 2% according to mass ratio calculating flavonoid, saponins, terpenoid, the content of steroid compound) for improving immunity of livestock；(3) Chinese herbal medicine micro-ecology fermentation mixture is being spray-dried, when pulverizing and slow release are coated granulation, it is not engaged in the herbal raw material (this part Chinese herbal medicine accounts for about the 35% of Chinese herbal medicine total amount) as substrate carries out fermenting before and participates in the course of processing as the coated carrier of slow release, this product thus can be made will not to be absorbed by poultry immediately after being eaten by poultry, but slowly release in the intestinal of poultry, on the one hand effective ingredient (the microecological microbial agent in product is made, herbal raw material and the produced specific biological active substances of fermentation) it can be avoided that destroyed by the gastric juice of poultry, the effective ingredient that can arrive action site is greatly increased；On the other hand effective ingredient in product is enable to be uniformly distributed in each position of animal and bird intestines along with the wriggling of animal and bird intestines, so each position microecological microbial agent field planting at animal and bird intestines becomes superior microorganism, herbal raw material and specific biological active substances and is fully absorbed the immunity for activating and strengthen poultry.
Detailed description of the invention
Below by specific embodiment, the present invention is further illustrated.
Embodiment one:
A) carry out pulverizing and being dried according to following ratio (mass ratio) by 450 kilograms of preferred Chinese herbal medicine: Flos Lonicerae 10 parts, Herba Taraxaci 10 parts, the Radix Astragali 10 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, 10 parts of Poria, 5 parts of Seem Lablab Album, Rhizoma Dioscoreae 5 parts, Fructus Crataegi 8 parts, Cortex Acanthopancis 7 parts, Rhizoma Atractylodis 7 parts, rush down pool 12 parts, Radix Polygoni Multiflori Preparata 6 parts, Herb Gynostemmae Pentaphylli 5 parts.
B) by 550 kilograms of microbial ecological agent according to following ratio (mass ratio) mix homogeneously: streptococcus faecalis 15 parts, bacillus subtilis 15 parts, Bacillus coagulans 25 parts, pediococcus acidilactici 10 parts, Lactobacillus plantarum 15 parts, 15 parts of yeast, ferrum glycinate 3 parts, tryptophan 2 parts.
C) herbal raw material prepared is mixed homogeneously according to the ratio of 45: 55 with microecological microbial agent.
D) complete herbal raw material mixed above and micro-ecological bacterial agent composition being positioned in the environment that room temperature is 37 degrees Celsius and carry out preculture, pre-incubated initial ph value is controlled about 6 by regulation.Incubation time is 60 hours.Through pre-incubated microecological microbial agent viable count more than or equal to 5 × 108CUF/g。
E) after preculture process, transferring them to ferment in solid fermentation bioreactor, fermentation temperature is 32 degrees Celsius.Fermentation time is 75 hours.
F), after fermentation, colony counting method and high performance liquid chromatography is used to detect microecological microbial agent viable count and the content of physiologically active substance of tunning respectively.
G) testing result: microecological microbial agent viable count is more than or equal to 2 × 109CUF/g, flavonoid, saponins, terpenoid, the content of steroid compound are respectively 1.8%, 2%, 2.5% and 1,5%, are removed by Chinese herbal medicine micro-ecological fermenting mixture from solid fermentation bioreactor.
H) the Chinese herbal medicine micro-ecological fermenting mixture being moved out of, through being spray-dried, is pulverized and is qualified finished product after being coated granulation with slow release.
I) by finished product according to approrpiate wts (case=20 bag × 1 kilogram/bag) subpackage, post label and can be used as commodity selling.
Embodiment two:
A) carry out pulverizing and being dried according to following ratio (mass ratio) by 500 kilograms of preferred Chinese herbal medicine: Flos Lonicerae 10 parts, Herba Taraxaci 10 parts, the Radix Astragali 10 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, 10 parts of Poria, 5 parts of Seem Lablab Album, Rhizoma Dioscoreae 5 parts, Fructus Crataegi 8 parts, Cortex Acanthopancis 7 parts, Rhizoma Atractylodis 7 parts, rush down pool 12 parts, Radix Polygoni Multiflori Preparata 6 parts, Herb Gynostemmae Pentaphylli 5 parts.
B) by 500 kilograms of microbial ecological agent according to following ratio (mass ratio) mix homogeneously: streptococcus faecalis 15 parts, bacillus subtilis 15 parts, Bacillus coagulans 25 parts, pediococcus acidilactici 10 parts, Lactobacillus plantarum 15 parts, 15 parts of yeast, ferrum glycinate 3 parts, tryptophan 2 parts.
C) herbal raw material prepared is mixed homogeneously according to the ratio of 50: 50 with microecological microbial agent.
D) complete herbal raw material mixed above and micro-ecological bacterial agent composition being positioned in the environment that room temperature is 37 degrees Celsius and carry out preculture, pre-incubated initial ph value is controlled about 5 by regulation.Incubation time is 65 hours.Through pre-incubated microecological microbial agent viable count more than or equal to 1.2 × 109CUF/g。
E) after preculture process, transferring them to ferment in solid fermentation bioreactor, fermentation temperature is 32 degrees Celsius.Fermentation time is 83 hours.
F), after fermentation, colony counting method and high performance liquid chromatography is used to detect microecological microbial agent viable count and the content of physiologically active substance of tunning respectively.
G) testing result: microecological microbial agent viable count is more than or equal to 4 × 109CUF/g, flavonoid, saponins, terpenoid, the content of steroid compound are respectively 2%, 3.5%, 2.5% and 1,5%, are removed by Chinese herbal medicine micro-ecological fermenting mixture from solid fermentation bioreactor.
H) the Chinese herbal medicine micro-ecological fermenting mixture being moved out of, through being spray-dried, is pulverized and is qualified finished product after being coated granulation with slow release.
I) by finished product according to approrpiate wts (case=20 bag × 1 kilogram/bag) subpackage, post label and can be used as commodity selling.
Embodiment three
A) carry out pulverizing and being dried according to following ratio (mass ratio) by 550 kilograms of preferred Chinese herbal medicine: Flos Lonicerae 10 parts, Herba Taraxaci 10 parts, the Radix Astragali 10 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, 10 parts of Poria, 5 parts of Seem Lablab Album, Rhizoma Dioscoreae 5 parts, Fructus Crataegi 8 parts, Cortex Acanthopancis 7 parts, Rhizoma Atractylodis 7 parts, rush down pool 12 parts, Radix Polygoni Multiflori Preparata 6 parts, Herb Gynostemmae Pentaphylli 5 parts.
B) by 450 kilograms of microbial ecological agent according to following ratio (mass ratio) mix homogeneously: streptococcus faecalis 15 parts, bacillus subtilis 15 parts, Bacillus coagulans 25 parts, pediococcus acidilactici 10 parts, Lactobacillus plantarum 15 parts, 15 parts of yeast, ferrum glycinate 3 parts, tryptophan 2 parts.
C) herbal raw material prepared is mixed homogeneously according to the ratio of 55: 45 with microecological microbial agent.
D) complete herbal raw material mixed above and micro-ecological bacterial agent composition being positioned in the environment that room temperature is 37 degrees Celsius and carry out preculture, pre-incubated initial ph value is controlled about 4.5 by regulation.Incubation time is 72 hours.Through pre-incubated microecological microbial agent viable count more than or equal to 2.7 × 109CUF/g。
E) after preculture process, transferring them to ferment in solid fermentation bioreactor, fermentation temperature is 32 degrees Celsius.Fermentation time is 96 hours.
F), after fermentation, colony counting method and high performance liquid chromatography is used to detect microecological microbial agent viable count and the content of physiologically active substance of tunning respectively.
G) testing result: microecological microbial agent viable count is more than or equal to 5.5 × 109CUF/g, flavonoid, saponins, terpenoid, the content of steroid compound are respectively 2%, 4%, 3% and 2%, are removed by Chinese herbal medicine micro-ecological fermenting mixture from solid fermentation bioreactor.
H) the Chinese herbal medicine micro-ecological fermenting mixture being moved out of, through being spray-dried, is pulverized and is qualified finished product after being coated granulation with slow release.
I) by finished product according to approrpiate wts (case=20 bag × 1 kilogram/bag) subpackage, post label and can be used as commodity selling.
Principle and the embodiment of the present invention are set forth by specific embodiment used herein, and the explanation of above example is only intended to help to understand method and the core concept thereof of the present invention.The above is only the preferred embodiment of the present invention, should be understood that, finiteness due to literal expression, and objectively there is unlimited concrete structure, for those skilled in the art, under the premise without departing from the principles of the invention, it is also possible to make some improvement, retouch or change, it is also possible to above-mentioned technical characteristic is combined by rights；These improve retouching, change or combine, or the most improved design by invention and technical scheme directly apply to other occasion, are regarded as protection scope of the present invention.
Claims (7)
1. a Chinese herbal medicine micro-ecological premixed feed, it is characterized in that, including herbal raw material and microbial bacterial agent, herbal raw material includes Flos Lonicerae 5-15 part, Herba Taraxaci 5-15 part, Radix Astragali 5-15 part, Rhizoma Atractylodis Macrocephalae 3-7 part, Poria 5-15 part, Seem Lablab Album 3-7 part, Rhizoma Dioscoreae 3-7 part, Fructus Crataegi 5-10 part, Cortex Acanthopancis 5-10 part, Rhizoma Atractylodis 5-10 part, rushes down pool 10-15 part, Radix Polygoni Multiflori Preparata 4-8 part, Herb Gynostemmae Pentaphylli 3-7 part；Microbial bacterial agent includes streptococcus faecalis 10-20 part, bacillus subtilis 10-20 part, Bacillus coagulans 10-20 part, pediococcus acidilactici 5-15 part, Lactobacillus plantarum 10-20 part, yeast 10-20 part, ferrum glycinate 2-4 part, tryptophan 1-3 part；Described herbal raw material is mixed in following ratio with described microbial bacterial agent: herbal raw material 45%-55%, microbial bacterial agent 45%-55%.
A kind of Chinese herbal medicine micro-ecological premixed feed the most according to claim 1, it is characterized in that, the formula of described herbal raw material is Flos Lonicerae 10 parts, Herba Taraxaci 10 parts, the Radix Astragali 10 parts, the Rhizoma Atractylodis Macrocephalae 5 parts, 10 parts of Poria, 5 parts of Seem Lablab Album, Rhizoma Dioscoreae 5 parts, Fructus Crataegi 8 parts, Cortex Acanthopancis 7 parts, Rhizoma Atractylodis 7 parts, rush down 12 parts of pool, Radix Polygoni Multiflori Preparata 6 parts, Herb Gynostemmae Pentaphylli 5 parts, the formula of described microbial bacterial agent is streptococcus faecalis 15 parts, bacillus subtilis 15 parts, Bacillus coagulans 25 parts, pediococcus acidilactici 10 parts, Lactobacillus plantarum 15 parts, 15 parts of yeast, ferrum glycinate 3 parts, tryptophan 2 parts.
Chinese herbal medicine micro-ecological premixed feed the most according to claim 1 and 2, it is characterized in that, also include maltose and Semen Maydis powder, the mixture quality of herbal raw material and microbial bacterial agent is A, the quality of maltose is B, the quality of Semen Maydis powder is C, in mixture A: B: C=100: 0.5-1.5: 1-3).
Chinese herbal medicine micro-ecological premixed feed the most according to claim 3, it is characterised in that A: B: C=100: 1: 1.5.
5. the preparation method of the Chinese herbal medicine micro-ecological premixed feed as according to any one of Claims 1-4, it is characterised in that comprise the following steps:
A. described herbal raw material is mixed with described microecological microbial agent；
B. by the mixture of said herbal medicine raw material and microecological microbial agent with maltose, Semen Maydis powder with 100: the ratio of 0.5-1.5: 1-3 mixes, and carries out preculture, and preculture temperature is 36-38 DEG C, and pre-incubation time is 60-75h；
C. fermenting, ferment at a temperature of 32-36 DEG C 75-96h.
The preparation method of Chinese herbal medicine micro-ecological premixed feed the most according to claim 5, it is characterised in that also include after step c that tunning is spray-dried by step d., pulverizes and discharges and be coated granulation.
The preparation method of Chinese herbal medicine micro-ecological premixed feed the most according to claim 6, it is characterized in that, include the step e. detection microecological microbial agent viable count of tunning and content of physiologically active substance between step c and d, detect qualified after be further continued for subsequent operation.
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| CN201511031933.8A CN105961829A (en) | 2015-12-28 | 2015-12-28 | Chinese herbal medicinal micro-ecological premixed feed and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106615857A (en) * | 2017-02-09 | 2017-05-10 | 安徽菲扬农业科技有限公司 | Black pig feed used in summer and preparation method thereof |
| CN107047945A (en) * | 2016-12-28 | 2017-08-18 | 新昌县柏克动物饲料技术开发有限公司 | A kind of Chinese herbal feed additive and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401921A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof |
| CN103549141A (en) * | 2013-04-25 | 2014-02-05 | 北京生泰尔生物科技有限公司 | Chinese herbal medicine composite probiotic feed additive for pigs and preparation method of additive |
| CN104397348A (en) * | 2014-12-26 | 2015-03-11 | 郭庆 | Preparation method of new castle disease traditional Chinese medicine feed additive |
| CN104585493A (en) * | 2015-01-30 | 2015-05-06 | 北京康华远景科技股份有限公司 | Plant microecological preparation for enhancing immunity of animal and preparation method of plant microecological preparation |
-
2015
- 2015-12-28 CN CN201511031933.8A patent/CN105961829A/en active Pending
- 2015-12-28 CN CN202211247947.3A patent/CN115606686A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401921A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof |
| CN103549141A (en) * | 2013-04-25 | 2014-02-05 | 北京生泰尔生物科技有限公司 | Chinese herbal medicine composite probiotic feed additive for pigs and preparation method of additive |
| CN104397348A (en) * | 2014-12-26 | 2015-03-11 | 郭庆 | Preparation method of new castle disease traditional Chinese medicine feed additive |
| CN104585493A (en) * | 2015-01-30 | 2015-05-06 | 北京康华远景科技股份有限公司 | Plant microecological preparation for enhancing immunity of animal and preparation method of plant microecological preparation |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047945A (en) * | 2016-12-28 | 2017-08-18 | 新昌县柏克动物饲料技术开发有限公司 | A kind of Chinese herbal feed additive and preparation method thereof |
| CN106615857A (en) * | 2017-02-09 | 2017-05-10 | 安徽菲扬农业科技有限公司 | Black pig feed used in summer and preparation method thereof |
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Effective date of registration: 20190227 Address after: 410000 Liudou'an Village, Xiaduopu Town, Ningxiang County, Changsha City, Hunan Province, 3 Groups Applicant after: Hunan Yisai Biotechnology Co.,Ltd. Address before: 410000 Wujialing Commercial Center, No. 151, Furong North Road, Kaifu District, Changsha City, Hunan Province, 5 buildings 919 Applicant before: HUNAN DARK HORSE ECOLOGICAL TECHNOLOGY CO.,LTD. |
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