CN106615808A - Pig feed additive capable of preventing and treating porcine respiratory syndromes and epidemic diarrhea - Google Patents
Pig feed additive capable of preventing and treating porcine respiratory syndromes and epidemic diarrhea Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/41—Crassulaceae (Stonecrop family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/487—Psoralea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/535—Perilla (beefsteak plant)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/69—Polygalaceae (Milkwort family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
The invention provides a pig feed additive capable of preventing and treating porcine respiratory syndromes and epidemic diarrhea. The pig feed additive capable of preventing and treating the porcine respiratory syndromes and epidemic diarrhea is composed of the following ingredients: compound plant extracts, fermented Chinese herbal medicine powder, a clostridium butyricum preparation, polygala root powder, and radix polygonati officinalis powder. The pig feed additive is capable of effectively preventing and treating the respiratory syndromes caused by bacteria and viruses, as well as effectively preventing and treating epidemic diarrhea of the pigs; moreover, the pig feed additive is safe, non-toxic, relatively small in addition amount, stable in quality, and capable of effectively improving breeding efficiency.
Description
Technical field
The present invention relates to a kind of feed addictive, more particularly to it is a kind of with preventing and treating porcine respiratory disease and epidemic diarrhea
Pig feed additive, belong to feed additive field.
Background technology
Porcine respiratory disease syndrome is a kind of general name by the multifactor breathing problem for causing, and is that growth bred pigs are universal
The disease of presence.The harm of this disease is that feed conversion rate is reduced, and poor growth, medical expense increases, and postpones listing, the quality of pig
Decline, several cases can cause the serious financial consequences such as death.
Diarrhoea is one of main clinic symptoms of chitling tract disease, caused by being intestinal absorption obstacle, with increased frequency of discharging feces,
The syndrome that excrement is thin, bloody stool is basic Clinical symptoms.Diarrhoea is not an independent disease, but is caused by many reasons
Or numerous disease with a kind of common multiple syndrome of clinic.Recent years, the incidence of disease of Porcine Epidemic Diarrhea is special
Height, especially the piglet at 1-3 monthly ages is relatively conventional, and the incidence of disease of piglet epidemic diarrhea is also up to 30%-40% after wean, dead
Rate is up to 30%-50%, and particularly sucking pig M & M is higher, up to 100%.The speed of growth is slow after pig morbidity, and feed turns
Rate is low, and piglet once infects this disease, and physique declines, immunocyte decline, for the resistance of disease weakens, easily secondary sense
Dye animal infectious diease, treatment not in time, will cause large quantities of death.
It is current most common method by medicine and antibiotic therapy breathing problem and diarrhoea, the method is in certain journey
There is certain effect on degree, but use in a large number for a long time, impact can be produced on the liver kidney of pig, cause immune decline, while medicine
Cause meat quality to decline with the residual of antibiotic, cause potential food-safety problem, environmental pollution is serious.And it is logical at present
Cross medicine to be mainly based on treatment, preventive effect is not good, and drug therapy effect is more single, such as respiratory syndrome is generally adopted
With aureomycin or terramycin or fortimicin treatment, epidemic diarrhea is frequently with antibiotic therapies such as tetracycline, gentamicins.Cause
This, developing one kind safely and efficiently can effectively prevent and treat porcine respiratory syndrome and can prevent and treat pig epidemic diarrhea additive again,
It is significant for raising pig-breeding economic benefit and social benefit.
The content of the invention
The purpose of the present invention occurs and existing medicine and antibiotic therapy for pig farm breathing problem and epidemic diarrhea
Disadvantage, there is provided one kind has preventing and treating porcine respiratory syndrome and epidemic diarrhea pig feed additive, for preventing and treating respiratory tract
The generation of disease and epidemic diarrhea, improves culture benefit.
The purpose of the present invention is realized by following technical scheme:
A kind of pig feed additive with preventing and treating porcine respiratory syndrome and epidemic diarrhea, it is characterised in that by following original
Material is constituted by weight:Composite vegetables extractive 10-16 parts, fermented Chinese herbal medicine powder 12-18 part, clostridium butyricum microbial inoculum 8-12 parts,
Polygala root powder 6-10 parts, radix polygonati officinalis powder 10-14 parts;
The composite vegetables extractive preparation method is as follows:Semen Lablab Album 5-7 parts are weighed, root of herbaceous peony 6-10 parts, Psoralea corylifolia 8-12 parts are red
Chinese herbaceous peony 4-6 parts, freeze 1-2h under the conditions of -20 DEG C, crush respectively, cross 60 mesh sieves, are well mixed, and 12-16 times of mixture of addition is weighed
The water of amount, maintains temperature 85-95 DEG C to extract 2-3h, filters to get filtrate, and the tannic acid of filtrate weight 0.1-0.2% is added in filtrate,
After standing 30min, filter residue is filtered to obtain, composite vegetables extractive is obtained after vacuum drying;
The fermentation of Chinese herbal medicine powder, preparation method thereof is as follows:Weigh setose thistle 8-10 parts respectively, root of kudzu vine 6-8 parts, rhodiola root 12-16 parts,
Perilla leaf 4-8 parts, pomace 10-16 parts, dregs of beans 8-12 parts crushed respectively 40 mesh sieves, were well mixed, and added in mixture
Distilled water, makes the water content of mixture up to 25-30%, adds the composite fermentation microbial inoculum of mixture weight 0.5%, sealing and fermenting 36-
48h, carries out low-temperature air-drying after fermentation ends, obtain fermented Chinese herbal medicine powder;
The composite fermentation microbial inoculum is by bacteroides ruminicola bacterium powder, lactobacillus acidophilus bacterium powder, Lactobacillus plantarum bacterium powder, the short bud of side spore
Spore bacillus bacterium powder compares 1 according to weight:0.5-1:1-2:0.8-1.2 is constituted;
The clostridium butyricum bacterial preparation process is as follows:
From inclined-plane switching culture clostridium butyricum, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, controls temperature for 30-35
DEG C, aerlbic culture 48-60 hours, 90 revs/min of mixing speed, ventilation(V/V)1:0.5, tank pressure 0.06MPa, fermentation is finished,
Spray drying makes clostridium butyricum microbial inoculum;
The slant medium percentage by weight is consisted of:Potato juice 20%, glucose 2%, agar 2.0%, rhodiola root powder
0.8%, bark of eucommia powder 1.2%, remaining is pure water;
The first order seed used that spreads cultivation step by step, secondary seed, three-level seed, first class seed pot, secondary seed tank and fermentation life
Produce used medium percentage by weight composition to be:Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, rhodiola root powder 0.8%, bark of eucommia powder 1.2%, Semen Lablab Album powder 1.8%, radix paeoniae rubrathe powder 1.2%, herbaceous peony powder 1.8%,
Remaining is pure water, pH 6.5;
Product preparation method of the present invention is as follows:
Composite vegetables extractive, fermented Chinese herbal medicine powder, clostridium butyricum microbial inoculum, polygala root powder, radix polygonati officinalis powder mix bacterium agent are obtained final product;
Addition of the present invention in swine rationses is 600-1200g/ tons.
Beneficial effect:
1. the composite vegetables extractive of the present invention contains the bioactivators such as various triterpene compounds, glucoside, flavones, polysaccharide,
With antibacterial, antiviral, enhancing immunity of organisms, promote effect of pig growth.Pass through freezing processing in its preparation method simultaneously
And add tannic acid to greatly improve the recovery rate of effective active composition in Chinese herbal medicine in extract, act on less with addition,
Composite vegetables extractive, fermentation of Chinese herbal medicine, clostridium butyricum and polygala root, radix polygonati officinalis etc. are realized into organic assembling, particularly be with the addition of
Traditional Chinese medicine ingredients and bacillus subtilis formulation, effectively reduce usage amount of the antibiotics in letting animals feed, butyric acid shuttle
Bacterium effective absorption and accumulation rhodiola root, the bark of eucommia, Semen Lablab Album, the radix paeoniae rubrathe, active component of the root of herbaceous peony in incubation, improve this
The biological effect of bright product.It is an advantage of the invention that being added in feed using Chinese herbal medicine extract, Chinese herbal medicine has both been improve
The property of medicine, can not only effectively prevent and treat various by the bacillary and viral respiratory syndrome for causing, moreover it is possible to which effectively preventing and treating pig is popular
Property diarrhoea effect, and safety non-toxic, addition is less, steady quality.
2nd, Chinese herbal medicine by fermentation after, active component is greatly improved, and macromolecular complex is changed into small-molecule substance, it is easier to pig
Absorption, while fermenting agent absorbs during the fermentation the drug effect of Chinese herbal medicine, while substantial amounts of active factors can be generated,
Immunity of organism is effectively improved, improves palatability, promote the growth of live pig.
3. clostridium butyricum has following function:A. promote propagation and the development of intestinal beneficial flora, suppress harmful bacteria and corruption
The growth of bacterium is lost, the generation of enterotoxin is reduced;B. metabolite contains B family vitamins, amylase etc., with health role;c.
Better tolerance, it is heat-resisting acidproof, do not affected by hydrochloric acid in gastric juice, bile acid etc., by alimentary canal non-inactivation;D. animal immunizing power is improved.
4. during invention, inventors be surprised to learn that, additive of the present invention has significant to the delicate flavour material of pork
Raising is acted on, and the content that can make pork Glutamic Acid and aspartic acid improves more than 30%.
Embodiment:
Embodiment 1
A kind of pig feed additive with preventing and treating porcine respiratory syndrome and epidemic diarrhea, it is characterised in that by following original
Material is constituted by weight:10 parts of composite vegetables extractive, 12 parts of fermented Chinese herbal medicine powder, 8 parts of clostridium butyricum microbial inoculum, 6 parts of polygala root powder,
10 parts of radix polygonati officinalis powder;
The composite vegetables extractive preparation method is as follows:Weigh 5 parts of Semen Lablab Album, 6 parts of the root of herbaceous peony, 8 parts of Psoralea corylifolia, 4 parts of the radix paeoniae rubrathe,
1h is freezed under the conditions of -20 DEG C, is crushed respectively, cross 60 mesh sieves, be well mixed, add the water of 12 times of weight of mixture, maintain temperature
85 DEG C of extraction 2h of degree, filter to get filtrate, and the tannic acid of filtrate weight 0.1% is added in filtrate, and after standing 30min, filtering to filter
Slag, obtains composite vegetables extractive after vacuum drying;
The fermentation of Chinese herbal medicine powder, preparation method thereof is as follows:8 parts of setose thistle, 6 parts of the root of kudzu vine, 12 parts of rhodiola root, perilla leaf 4 are weighed respectively
Part, 10 parts of pomace, 8 parts of dregs of beans was crushed respectively 40 mesh sieves, was well mixed, and in mixture distilled water is added, and made mixture
Water content up to 25%, add the composite fermentation microbial inoculum of mixture weight 0.5%, sealing and fermenting 36h that low temperature is carried out after fermentation ends
Air-dry, obtain fermented Chinese herbal medicine powder;
The composite fermentation microbial inoculum is by bacteroides ruminicola bacterium powder, lactobacillus acidophilus bacterium powder, Lactobacillus plantarum bacterium powder, the short bud of side spore
Spore bacillus bacterium powder compares 1 according to weight:0.5:1:0.8 composition;
The clostridium butyricum bacterial preparation process is as follows:
From inclined-plane switching culture clostridium butyricum, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, controls temperature for 30 DEG C, is led to
Wind culture 48 hours, 90 revs/min of mixing speed, ventilation(V/V)1:0.5, tank pressure 0.06MPa, fermentation is finished, and is spray-dried
Make clostridium butyricum microbial inoculum;
The slant medium percentage by weight is consisted of:Potato juice 20%, glucose 2%, agar 2.0%, rhodiola root powder
0.8%, bark of eucommia powder 1.2%, remaining is pure water;
The first order seed used that spreads cultivation step by step, secondary seed, three-level seed, first class seed pot, secondary seed tank and fermentation life
Produce used medium percentage by weight composition to be:Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, rhodiola root powder 0.8%, bark of eucommia powder 1.2%, Semen Lablab Album powder 1.8%, radix paeoniae rubrathe powder 1.2%, herbaceous peony powder 1.8%,
Remaining is pure water, pH 6.5;
Product preparation method of the present invention is as follows:
Composite vegetables extractive, fermented Chinese herbal medicine powder, clostridium butyricum microbial inoculum, polygala root powder, radix polygonati officinalis powder mix bacterium agent are obtained final product;
Addition of the present invention in swine rationses is 1200g/ tons.
Embodiment 2
A kind of pig feed additive with preventing and treating porcine respiratory syndrome and epidemic diarrhea, it is characterised in that by following original
Material is constituted by weight:13 parts of composite vegetables extractive, 15 parts of fermented Chinese herbal medicine powder, 10 parts of clostridium butyricum microbial inoculum, polygala root powder 8
Part, 12 parts of radix polygonati officinalis powder;
The composite vegetables extractive preparation method is as follows:Weigh 6 parts of Semen Lablab Album, 8 parts of the root of herbaceous peony, 10 parts of Psoralea corylifolia, 5 parts of the radix paeoniae rubrathe,
1.5h is freezed under the conditions of -20 DEG C, is crushed respectively, cross 60 mesh sieves, be well mixed, add the water of 14 times of weight of mixture, maintained
90 DEG C of extraction 2.5h of temperature, filter to get filtrate, and the tannic acid of filtrate weight 0.15% is added in filtrate, after standing 30min, filter
Filter residue is obtained, composite vegetables extractive is obtained after vacuum drying;
The fermentation of Chinese herbal medicine powder, preparation method thereof is as follows:9 parts of setose thistle, 7 parts of the root of kudzu vine, 14 parts of rhodiola root, perilla leaf 6 are weighed respectively
Part, 13 parts of pomace, 10 parts of dregs of beans was crushed respectively 40 mesh sieves, was well mixed, and in mixture distilled water is added, and made mixing
The water content of thing adds the composite fermentation microbial inoculum of mixture weight 0.5%, sealing and fermenting 42h to carry out after fermentation ends low up to 28%
Warm air is done, and obtains fermented Chinese herbal medicine powder;
The composite fermentation microbial inoculum is by bacteroides ruminicola bacterium powder, lactobacillus acidophilus bacterium powder, Lactobacillus plantarum bacterium powder, the short bud of side spore
Spore bacillus bacterium powder compares 1 according to weight:0.8:1.5:1 composition;
The clostridium butyricum bacterial preparation process is as follows:
From inclined-plane switching culture clostridium butyricum, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, controls temperature for 33 DEG C, is led to
Wind culture 54 hours, 90 revs/min of mixing speed, ventilation(V/V)1:0.5, tank pressure 0.06MPa, fermentation is finished, and is spray-dried
Make clostridium butyricum microbial inoculum;
The slant medium percentage by weight is consisted of:Potato juice 20%, glucose 2%, agar 2.0%, rhodiola root powder
0.8%, bark of eucommia powder 1.2%, remaining is pure water;
The first order seed used that spreads cultivation step by step, secondary seed, three-level seed, first class seed pot, secondary seed tank and fermentation life
Produce used medium percentage by weight composition to be:Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, rhodiola root powder 0.8%, bark of eucommia powder 1.2%, Semen Lablab Album powder 1.8%, radix paeoniae rubrathe powder 1.2%, herbaceous peony powder 1.8%,
Remaining is pure water, pH 6.5;
Product preparation method of the present invention is as follows:
Composite vegetables extractive, fermented Chinese herbal medicine powder, clostridium butyricum microbial inoculum, polygala root powder, radix polygonati officinalis powder mix bacterium agent are obtained final product;
Addition of the present invention in swine rationses is 900g/ tons.
Embodiment 3
A kind of pig feed additive with preventing and treating porcine respiratory syndrome and epidemic diarrhea, it is characterised in that by following original
Material is constituted by weight:16 parts of composite vegetables extractive, 18 parts of fermented Chinese herbal medicine powder, 12 parts of clostridium butyricum microbial inoculum, polygala root powder 10
Part, 14 parts of radix polygonati officinalis powder;
The composite vegetables extractive preparation method is as follows:Weigh 7 parts of Semen Lablab Album, 10 parts of the root of herbaceous peony, 12 parts of Psoralea corylifolia, 6 parts of the radix paeoniae rubrathe,
2h is freezed under the conditions of -20 DEG C, is crushed respectively, cross 60 mesh sieves, be well mixed, add the water of 16 times of weight of mixture, maintain temperature
95 DEG C of extraction 3h of degree, filter to get filtrate, and the tannic acid of filtrate weight 0.2% is added in filtrate, and after standing 30min, filtering to filter
Slag, obtains composite vegetables extractive after vacuum drying;
The fermentation of Chinese herbal medicine powder, preparation method thereof is as follows:10 parts of setose thistle, 8 parts of the root of kudzu vine, 16 parts of rhodiola root, perilla leaf 8 are weighed respectively
Part, 16 parts of pomace, 12 parts of dregs of beans was crushed respectively 40 mesh sieves, was well mixed, and in mixture distilled water is added, and made mixing
The water content of thing adds the composite fermentation microbial inoculum of mixture weight 0.5%, sealing and fermenting 48h to carry out after fermentation ends low up to 30%
Warm air is done, and obtains fermented Chinese herbal medicine powder;
The composite fermentation microbial inoculum is by bacteroides ruminicola bacterium powder, lactobacillus acidophilus bacterium powder, Lactobacillus plantarum bacterium powder, the short bud of side spore
Spore bacillus bacterium powder compares 1 according to weight: 1: 2:1.2 compositions;
The clostridium butyricum bacterial preparation process is as follows:
From inclined-plane switching culture clostridium butyricum, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, controls temperature for 35 DEG C, is led to
Wind culture 60 hours, 90 revs/min of mixing speed, ventilation(V/V)1:0.5, tank pressure 0.06MPa, fermentation is finished, and is spray-dried
Make clostridium butyricum microbial inoculum;
The slant medium percentage by weight is consisted of:Potato juice 20%, glucose 2%, agar 2.0%, rhodiola root powder
0.8%, bark of eucommia powder 1.2%, remaining is pure water;
The first order seed used that spreads cultivation step by step, secondary seed, three-level seed, first class seed pot, secondary seed tank and fermentation life
Produce used medium percentage by weight composition to be:Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, rhodiola root powder 0.8%, bark of eucommia powder 1.2%, Semen Lablab Album powder 1.8%, radix paeoniae rubrathe powder 1.2%, herbaceous peony powder 1.8%,
Remaining is pure water, pH 6.5;
Product preparation method of the present invention is as follows:
Composite vegetables extractive, fermented Chinese herbal medicine powder, clostridium butyricum microbial inoculum, polygala root powder, radix polygonati officinalis powder mix bacterium agent are obtained final product;
Addition of the present invention in swine rationses is 600g/ tons.
Embodiment 4
A kind of pig feed additive with preventing and treating porcine respiratory syndrome and epidemic diarrhea, it is characterised in that by following original
Material is constituted by weight:86 parts of composite vegetables extractive, 20 parts of fermented Chinese herbal medicine powder, 24 parts of clostridium butyricum microbial inoculum, polygala root powder 2
Part, 8 parts of radix polygonati officinalis powder;
Remaining is with embodiment 2.
Embodiment 5
A kind of pig feed additive with preventing and treating porcine respiratory syndrome and epidemic diarrhea, it is characterised in that by following original
Material is constituted by weight:13 parts of composite vegetables extractive, 15 parts of fermented Chinese herbal medicine powder, 10 parts of clostridium butyricum microbial inoculum, polygala root powder 8
Part, 12 parts of radix polygonati officinalis powder;
The composite vegetables extractive preparation method is as follows:Weigh 4 parts of Semen Lablab Album, 12 parts of the root of herbaceous peony, 15 parts of Psoralea corylifolia, 2 parts of the radix paeoniae rubrathe,
0.5h is freezed under the conditions of -20 DEG C, is crushed respectively, cross 60 mesh sieves, be well mixed, add the water of 10 times of weight of mixture, maintained
80 DEG C of extraction 4h of temperature, filter to get filtrate, and the tannic acid of filtrate weight 0.05% is added in filtrate, after standing 30min, filter
Filter residue, obtains composite vegetables extractive after vacuum drying;
The fermentation of Chinese herbal medicine powder, preparation method thereof is as follows:12 parts of setose thistle, 14 parts of the root of kudzu vine, 18 parts of rhodiola root, perilla leaf 2 are weighed respectively
Part, 18 parts of pomace, 6 parts of dregs of beans was crushed respectively 40 mesh sieves, was well mixed, and in mixture distilled water is added, and made mixture
Water content up to 35%, add the composite fermentation microbial inoculum of mixture weight 0.5%, sealing and fermenting 50h that low temperature is carried out after fermentation ends
Air-dry, obtain fermented Chinese herbal medicine powder;
The composite fermentation microbial inoculum is by bacteroides ruminicola bacterium powder, lactobacillus acidophilus bacterium powder, Lactobacillus plantarum bacterium powder, the short bud of side spore
Spore bacillus bacterium powder compares 1 according to weight:2:0.5:0.5 composition;
The clostridium butyricum bacterial preparation process is as follows:
From inclined-plane switching culture clostridium butyricum, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, controls temperature for 28 DEG C, is led to
Wind culture 40 hours, 90 revs/min of mixing speed, ventilation(V/V)1:0.5, tank pressure 0.06MPa, fermentation is finished, and is spray-dried
Make clostridium butyricum microbial inoculum;
The slant medium percentage by weight is consisted of:Potato juice 20%, glucose 2%, agar 2.0%, rhodiola root powder
0.8%, bark of eucommia powder 1.2%, remaining is pure water;
The first order seed used that spreads cultivation step by step, secondary seed, three-level seed, first class seed pot, secondary seed tank and fermentation life
Produce used medium percentage by weight composition to be:Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, rhodiola root powder 0.8%, bark of eucommia powder 1.2%, Semen Lablab Album powder 1.8%, radix paeoniae rubrathe powder 1.2%, herbaceous peony powder 1.8%,
Remaining is pure water, pH 6.5;
Product preparation method of the present invention is as follows:
Composite vegetables extractive, fermented Chinese herbal medicine powder, clostridium butyricum microbial inoculum, polygala root powder, radix polygonati officinalis powder mix bacterium agent are obtained final product;
Addition of the present invention in swine rationses is 1400g/ tons.
Embodiment 6
It is a kind of according to disclosed in the Chinese patent of Application No. 201310106335.7 effectively to prevent and treat in pig epidemic diarrhea
Herb extracts feed addictive, is made up of the raw material of following parts by weight:15 parts of accumulated snow grass meal;10 parts of curcuma powder;Cassia seed
15 parts of powder;20 parts of great burdock achene powder;15 parts of macrostem onion powder;35 parts of Chinese herbal and crude drugs preparations;10 parts of Turmeric P.E;35 parts of hickory chick microbial inoculum;
10 parts of bacillus subtilis microbial agent.Addition in swine rationses is 0.8g/kg.
Embodiment 7
A kind of effectively preventing and treating pig synthetic respiratory disease according to disclosed in the Chinese patent of Application No. 201310135953.4
Chinese herb feed additive, be made up of the raw material of following parts by weight:Plant polyose powder 15-25 parts, Herba Cirsii extract 8-15 parts,
Rhizoma cyperi crushed material 5-10 parts, lucid asparagus crushed material 6-10 parts, Radix Platycodonis extract 10-30 parts, mycelium powder of sinensis 10-25 parts,
Licorice powder 2-5 parts.Addition in swine rationses is 0.8g/kg.
Test example:
Experimental animal selects the sodium selenite that Meizhou subordinate plant of Twins Feed Co., Ltd. average weight is 7kg or so
400, statistical analysis weight differences are not notable.It is each by male and female by 400 sodium selenites using single-factor Randomized Designs
Half, it is divided into 8 groups, respectively 1 control group and 8 test groups, test group adds respectively feed addition obtained in embodiment 1-7
Agent, control group is without arbitrary additive obtained in embodiment 1-7.Per group of 5 repetitions, each repeats 10 pigs.Continuous feeding
It is 15 days, as follows to grice diarrhoea rate and respiratory disorder impact effect using invention feed addictive compared with control group:
Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 |
The breathing problem incidence of disease(%) | 31.4 | 4.5 | 4.0 | 4.6 | 27.6 | 28.4 | 28.4 | 6.2 |
Diarrhea rate(%) | 28.4 | 3.2 | 2.8 | 3.4 | 26.4 | 27.4 | 5.5 | 26.4 |
In live pig feeding experiment, embodiment 1-3(Each technical parameter is in the range of the present invention program)Corresponding test group
Compared with control group, breathing problem incidence and diarrhea rate are remarkably decreased, also below embodiment 7 and embodiment 8.Embodiment 6
Test porcine respiratory disease incidence is very high, with control group difference less, illustrates the respiratory tract disease of the additive to pig of embodiment 6
Sick preventive and therapeutic effect very little, embodiment 7 is very high to test pig diarrhea rate, with control group difference less, illustrates the additive of embodiment 7
To diarrhea of pigs preventive and therapeutic effect very little.The corresponding test group of embodiment 2 is to testing porcine respiratory disease and diarrhoea prevention effect most
It is good.
Claims (6)
1. it is a kind of with the pig feed additive for preventing and treating porcine respiratory syndrome and epidemic diarrhea, it is characterised in that by following
Raw material is constituted by weight:Composite vegetables extractive 10-16 parts, fermented Chinese herbal medicine powder 12-18 part, clostridium butyricum microbial inoculum 8-12
Part, polygala root powder 6-10 parts, radix polygonati officinalis powder 10-14 parts.
2. a kind of addition of the pig's feed with preventing and treating porcine respiratory syndrome and epidemic diarrhea according to claim 1
Agent, it is characterised in that:
The composite vegetables extractive preparation method is as follows:Semen Lablab Album 5-7 parts are weighed, root of herbaceous peony 6-10 parts, Psoralea corylifolia 8-12 parts are red
Chinese herbaceous peony 4-6 parts, freeze 1-2h under the conditions of -20 DEG C, crush respectively, cross 60 mesh sieves, are well mixed, and 12-16 times of mixture of addition is weighed
The water of amount, maintains temperature 85-95 DEG C to extract 2-3h, filters to get filtrate, and the tannic acid of filtrate weight 0.1-0.2% is added in filtrate,
After standing 30min, filter residue is filtered to obtain, composite vegetables extractive is obtained after vacuum drying;
The fermentation of Chinese herbal medicine powder, preparation method thereof is as follows:Weigh setose thistle 8-10 parts respectively, root of kudzu vine 6-8 parts, rhodiola root 12-16 parts,
Perilla leaf 4-8 parts, pomace 10-16 parts, dregs of beans 8-12 parts crushed respectively 40 mesh sieves, were well mixed, and added in mixture
Distilled water, makes the water content of mixture up to 25-30%, adds the composite fermentation microbial inoculum of mixture weight 0.5%, sealing and fermenting 36-
48h, carries out low-temperature air-drying after fermentation ends, obtain fermented Chinese herbal medicine powder;
The composite fermentation microbial inoculum is by bacteroides ruminicola bacterium powder, lactobacillus acidophilus bacterium powder, Lactobacillus plantarum bacterium powder, the short bud of side spore
Spore bacillus bacterium powder compares 1 according to weight:0.5-1:1-2:0.8-1.2 is constituted;
The clostridium butyricum bacterial preparation process is as follows:
From inclined-plane switching culture clostridium butyricum, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, controls temperature for 30-35
DEG C, aerlbic culture 48-60 hours, 90 revs/min of mixing speed, ventilation(V/V)1:0.5, tank pressure 0.06MPa, fermentation is finished,
Spray drying makes clostridium butyricum microbial inoculum;
The slant medium percentage by weight is consisted of:Potato juice 20%, glucose 2%, agar 2.0%, rhodiola root powder
0.8%, bark of eucommia powder 1.2%, remaining is pure water;
The first order seed used that spreads cultivation step by step, secondary seed, three-level seed, first class seed pot, secondary seed tank and fermentation life
Produce used medium percentage by weight composition to be:Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, rhodiola root powder 0.8%, bark of eucommia powder 1.2%, Semen Lablab Album powder 1.8%, radix paeoniae rubrathe powder 1.2%, herbaceous peony powder 1.8%,
Remaining is pure water, pH 6.5;
Product preparation method of the present invention is as follows:
Composite vegetables extractive, fermented Chinese herbal medicine powder, clostridium butyricum microbial inoculum, polygala root powder, radix polygonati officinalis powder mix bacterium agent are obtained final product.
3. a kind of addition of the pig's feed with preventing and treating porcine respiratory syndrome and epidemic diarrhea according to claim 1
Agent, it is characterised in that be made up of by weight following raw material:13 parts of composite vegetables extractive, 15 parts of fermented Chinese herbal medicine powder, butyric acid
10 parts of clostridium microbial inoculum, 8 parts of polygala root powder, 12 parts of radix polygonati officinalis powder.
4. a kind of addition of the pig's feed with preventing and treating porcine respiratory syndrome and epidemic diarrhea according to claim 1
Agent, it is characterised in that:The composite vegetables extractive preparation method is as follows:Weigh 6 parts of Semen Lablab Album, 8 parts of the root of herbaceous peony, Psoralea corylifolia 10
Part, 5 parts of the radix paeoniae rubrathe freezes 1.5h under the conditions of -20 DEG C, crushes respectively, crosses 60 mesh sieves, is well mixed, and adds 14 times of weights of mixture
The water of amount, maintains 90 DEG C of extraction 2.5h of temperature, filters to get filtrate, and the tannic acid of filtrate weight 0.15% is added in filtrate, stands
After 30min, filter residue is filtered to obtain, composite vegetables extractive is obtained after vacuum drying;
The fermentation of Chinese herbal medicine powder, preparation method thereof is as follows:9 parts of setose thistle, 7 parts of the root of kudzu vine, 14 parts of rhodiola root, perilla leaf 6 are weighed respectively
Part, 13 parts of pomace, 10 parts of dregs of beans was crushed respectively 40 mesh sieves, was well mixed, and in mixture distilled water is added, and made mixing
The water content of thing adds the composite fermentation microbial inoculum of mixture weight 0.5%, sealing and fermenting 42h to carry out after fermentation ends low up to 28%
Warm air is done, and obtains fermented Chinese herbal medicine powder;
The composite fermentation microbial inoculum is by bacteroides ruminicola bacterium powder, lactobacillus acidophilus bacterium powder, Lactobacillus plantarum bacterium powder, the short bud of side spore
Spore bacillus bacterium powder compares 1 according to weight:0.8:1.5:1 composition;
The clostridium butyricum bacterial preparation process is as follows:
From inclined-plane switching culture clostridium butyricum, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, controls temperature for 33 DEG C, is led to
Wind culture 54 hours, 90 revs/min of mixing speed, ventilation(V/V)1:0.5, tank pressure 0.06MPa, fermentation is finished, and is spray-dried
Make clostridium butyricum microbial inoculum;
The slant medium percentage by weight is consisted of:Potato juice 20%, glucose 2%, agar 2.0%, rhodiola root powder
0.8%, bark of eucommia powder 1.2%, remaining is pure water;
The first order seed used that spreads cultivation step by step, secondary seed, three-level seed, first class seed pot, secondary seed tank and fermentation life
Produce used medium percentage by weight composition to be:Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, rhodiola root powder 0.8%, bark of eucommia powder 1.2%, Semen Lablab Album powder 1.8%, radix paeoniae rubrathe powder 1.2%, herbaceous peony powder 1.8%,
Remaining is pure water, pH 6.5;
Product preparation method of the present invention is as follows:
Composite vegetables extractive, fermented Chinese herbal medicine powder, clostridium butyricum microbial inoculum, polygala root powder, radix polygonati officinalis powder mix bacterium agent are obtained final product.
5. a kind of addition of the pig's feed with preventing and treating porcine respiratory syndrome and epidemic diarrhea according to claim 1
Agent, it is characterised in that:Addition of the present invention in swine rationses is 600-1200g/ tons.
6. a kind of addition of the pig's feed with preventing and treating porcine respiratory syndrome and epidemic diarrhea according to claim 5
Agent, it is characterised in that:Addition of the present invention in swine rationses is 900g/ tons.
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CN108272788A (en) * | 2017-12-29 | 2018-07-13 | 武汉轻工大学 | Puerarin is in the drug and pig feed additive for preparing the purposes in preventing the drug that swine disease poison infects, prevention swine disease poison infection |
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CN111011627A (en) * | 2019-12-18 | 2020-04-17 | 成都大帝汉克生物科技有限公司 | Feed additive capable of preventing diarrhea and preparation method thereof |
CN111838460A (en) * | 2019-04-24 | 2020-10-30 | 山东瑞力生药业股份有限公司 | Chinese herbal medicine feed additive used for piglet stage and preparation method thereof |
CN115444876A (en) * | 2022-09-07 | 2022-12-09 | 华中农业大学 | Fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus and preparation method thereof |
CN115633735A (en) * | 2022-09-27 | 2023-01-24 | 翁源县新南都饲料科技有限公司 | Plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity |
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CN108272788B (en) * | 2017-12-29 | 2019-02-22 | 武汉轻工大学 | Puerarin is in the drug and pig feed additive for preparing the purposes in the drug for preventing and treating pig virus infection, preventing and treating pig virus infection |
CN108403753A (en) * | 2018-05-17 | 2018-08-17 | 四川长重宝生物工程有限责任公司 | The fermented tcm and preparation method thereof of prevention blue otopathy and circovirus |
CN111838460A (en) * | 2019-04-24 | 2020-10-30 | 山东瑞力生药业股份有限公司 | Chinese herbal medicine feed additive used for piglet stage and preparation method thereof |
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CN115444876A (en) * | 2022-09-07 | 2022-12-09 | 华中农业大学 | Fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus and preparation method thereof |
CN115444876B (en) * | 2022-09-07 | 2024-02-02 | 华中农业大学 | Fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus and preparation method thereof |
CN115633735A (en) * | 2022-09-27 | 2023-01-24 | 翁源县新南都饲料科技有限公司 | Plant extract feed additive for preventing and treating porcine respiratory diseases and enhancing immunity |
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