CN115444876B - Fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus and preparation method thereof - Google Patents
Fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 49
- 241001135549 Porcine epidemic diarrhea virus Species 0.000 title claims abstract description 42
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- 230000004151 fermentation Effects 0.000 claims abstract description 48
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A61K36/284—Atractylodes
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract
The invention discloses a fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus and a preparation method thereof, wherein the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus comprises Chinese herbal medicines and probiotics; the Chinese herbal medicine comprises the following components: astragalus root, pilose asiabell root, red paeony root, white paeony root and largehead atractylodes rhizome; the probiotics include: bacillus subtilis, saccharomyces cerevisiae, enterococcus faecalis. The invention adopts the traditional Chinese medicine preparation to antagonize porcine epidemic diarrhea virus, and eliminates the bitter taste of the traditional Chinese medicine and improves the palatability through microorganism fermentation.
Description
Technical Field
The invention relates to the technical field of livestock breeding, in particular to a fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus and a preparation method thereof.
Background
Porcine Epidemic Diarrhea Virus (PEDV) belongs to the genus coronavirus, causing watery diarrhea, vomiting and dehydration in live pigs. The symptoms of piglets are heavier than those of adult pigs, and the mortality rate is usually 50%. The adult pigs have no symptoms, are even or show depression, anorexia and vomiting, and can be ill by the infection of the digestive tract such as the feces of infected pigs, the ill of the piglets of all ages is the heaviest, the ill rate can reach 100 percent, and the ill rate of the sows is 15 to 90 percent. At present, no effective prevention and control medicine exists, and the antibiotic treatment can only slow down secondary infection. The existing researches show that compared with vaccine strains, the current PEDV new epidemic strains have larger variation, so that the immune protection effect of the existing commercial vaccines is not ideal, and the new epidemic strains are difficult to separate, domesticate and apply. The Chinese herbal medicine has the advantages of no residue, small side effect, difficult repetition and the like, so that the Chinese herbal medicine becomes one of important means for preventing and treating the virus. The Chinese herbal medicine affects the feeding of the live pigs due to the bitter taste. Therefore, the development of the fermented traditional Chinese medicine with good palatability and antagonism to porcine epidemic diarrhea virus has wide market prospect.
Disclosure of Invention
The fermented traditional Chinese medicine preparation for resisting the porcine epidemic diarrhea virus has good palatability and can antagonize the porcine epidemic diarrhea virus.
The technical scheme of the invention is as follows:
a fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus, which comprises Chinese herbal medicines and probiotics;
the Chinese herbal medicine comprises the following components: astragalus root, pilose asiabell root, red paeony root, white paeony root and largehead atractylodes rhizome;
the probiotics include: bacillus subtilis, saccharomyces cerevisiae, enterococcus faecalis.
Preferably, the Chinese herbal medicine comprises the following components in parts by weight: 20-30 parts of astragalus, 10-20 parts of radix codonopsis, 10-15 parts of red paeony root, 20-25 parts of white paeony root and 10-25 parts of largehead atractylodes rhizome; the probiotics comprise the following components in parts by weight: 0.1-0.2 part of bacillus subtilis, 0.1-0.2 part of saccharomyces cerevisiae and 0.1-0.2 part of enterococcus faecalis.
The preparation method of the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus comprises the following steps:
step (1): mixing the Chinese herbal medicines, crushing to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, and curing the mixed solution to obtain a Chinese herbal medicine mixture;
step (2): adding the probiotics into the traditional Chinese medicine mixture, and fermenting raw materials to obtain raw material fermentation liquid;
step (3): and (3) drying the raw material fermentation broth to obtain the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus.
Preferably, in the step (1), when the mixture A is mixed with water, the volume ratio of the mixture A to the water is 10:3.
Preferably, in the step (1), 10-30 parts by weight of any one of corn flour, bran or rice flour is added to the prepared mixture A and then mixed with water.
Preferably, in step (1), the curing treatment method includes: the mixture was steamed and heated to 80℃for 20min.
Preferably, in the step (2), the probiotics are added into the traditional Chinese medicine mixture at the temperature of 30-35 ℃, the weight ratio of the probiotics to the traditional Chinese medicine mixture is (3-4): 13, and raw material fermentation is carried out.
Preferably, in the step (2), the temperature of raw material fermentation is 35-37 ℃, and the time of raw material fermentation is 36 hours, so as to obtain raw material fermentation liquid.
Preferably, the preparation method of the probiotics comprises the following steps:
inoculating the enterococcus faecalis into a lactobacillus culture medium according to the inoculation amount of 0.5%, and performing heat preservation and activation for 2 hours at 30+/-5 ℃ to obtain an activation solution of the enterococcus faecalis;
inoculating the bacillus subtilis into a bacillus subtilis culture medium according to an inoculum size of 1%, and performing heat preservation and activation for 2 hours at 35+/-2 ℃ to obtain an activating solution of the bacillus subtilis;
inoculating the saccharomyces cerevisiae into a fungus culture medium according to an inoculum size of 0.2%, and performing heat preservation and activation for 2 hours at 28+/-2 ℃ to obtain an activating solution of the saccharomyces cerevisiae;
the probiotics are prepared by mixing 0.1-0.2 part of the prepared enterococcus faecalis activating solution, 0.1-0.2 part of bacillus subtilis activating solution and 0.1-0.2 part of saccharomyces cerevisiae activating solution in parts by weight.
Preferably, in step (3), drying the raw material fermentation broth includes: drying the raw material fermentation broth at 240-260 ℃ for 10s.
The invention has the beneficial effects that: the probiotics in the raw materials take Chinese herbal medicines as fermentation substrates, aromatic substances such as acetic acid, ethanol and the like are generated in the fermentation process, and the aromatic substances such as ethanol and the like can reduce and eliminate bitter and astringent smell of the Chinese herbal medicines, so that the feed is convenient for the pigs to eat. Under the fermentation action of probiotics, the cell wall of the traditional Chinese medicine plant can be promoted to be broken. The composition of radix astragali, radix Codonopsis, radix Paeoniae Rubra, radix Paeoniae alba and Atractylodis rhizoma can antagonize porcine epidemic diarrhea virus and treat porcine epidemic diarrhea.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in further detail below.
Example 1
A fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus, which comprises Chinese herbal medicines and probiotics;
the Chinese herbal medicine comprises the following components: astragalus root, pilose asiabell root, red paeony root, white paeony root and largehead atractylodes rhizome;
the probiotics include: bacillus subtilis, saccharomyces cerevisiae, enterococcus faecalis.
The Chinese herbal medicine comprises the following components in parts by weight: 20 parts of astragalus, 17 parts of codonopsis pilosula, 11 parts of red paeony root, 25 parts of white paeony root and 25 parts of largehead atractylodes rhizome; the probiotics comprise the following components in parts by weight: 0.2 part of bacillus subtilis, 0.11 part of saccharomyces cerevisiae and 0.16 part of enterococcus faecalis.
The preparation method of the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus comprises the following steps:
step (1): mixing the Chinese herbal medicines, crushing to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, and curing the mixed solution to obtain a Chinese herbal medicine mixture;
step (2): adding the probiotics into the traditional Chinese medicine mixture, and fermenting raw materials to obtain raw material fermentation liquid;
step (3): and (3) drying the raw material fermentation broth to obtain the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus.
In the step (1), when the mixture A is mixed with water, the volume ratio of the mixture A to the water is 10:3.
In the step (1), 24 parts by weight of corn flour is added to the prepared mixture A and then mixed with water.
In step (1), the curing treatment method comprises the following steps: the mixture was steamed and heated to 80℃for 20min.
In the step (2), the probiotics are added into the traditional Chinese medicine mixture at the temperature of 32 ℃, the weight ratio of the probiotics to the traditional Chinese medicine mixture is 3.5:13, and raw material fermentation is carried out.
In the step (2), the temperature of raw material fermentation is 36 ℃, the time of raw material fermentation is 36 hours, and raw material fermentation liquid is obtained.
The preparation method of the probiotics comprises the following steps:
inoculating the enterococcus faecalis into a lactobacillus culture medium according to the inoculation amount of 0.5%, and performing heat preservation and activation for 2 hours at 25 ℃ to obtain an activation solution of the enterococcus faecalis;
inoculating the bacillus subtilis into a bacillus subtilis culture medium according to an inoculum size of 1%, and performing heat preservation and activation for 2 hours at 35 ℃ to obtain an activating solution of the bacillus subtilis;
inoculating the saccharomyces cerevisiae into a fungus culture medium according to an inoculum size of 0.2%, and performing heat preservation and activation for 2 hours at the temperature of 28 ℃ to obtain an activating solution of the saccharomyces cerevisiae;
the probiotics are prepared by mixing 0.2 part of the prepared enterococcus faecalis activating solution, 0.1 part of bacillus subtilis activating solution and 0.18 part of saccharomyces cerevisiae activating solution in parts by weight.
In step (3), drying the raw material fermentation broth comprises: the raw material fermentation broth was dried at 260 ℃ for 10s.
Example 2
A fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus, which comprises Chinese herbal medicines and probiotics;
the Chinese herbal medicine comprises the following components: astragalus root, pilose asiabell root, red paeony root, white paeony root and largehead atractylodes rhizome;
the probiotics include: bacillus subtilis, saccharomyces cerevisiae, enterococcus faecalis.
The Chinese herbal medicine comprises the following components in parts by weight: 30 parts of astragalus, 10 parts of codonopsis pilosula, 15 parts of red paeony root, 20 parts of white paeony root and 17 parts of largehead atractylodes rhizome; the probiotics comprise the following components in parts by weight: 0.17 part of bacillus subtilis, 0.1 part of saccharomyces cerevisiae and 0.1 part of enterococcus faecalis.
The preparation method of the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus comprises the following steps:
step (1): mixing the Chinese herbal medicines, crushing to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, and curing the mixed solution to obtain a Chinese herbal medicine mixture;
step (2): adding the probiotics into the traditional Chinese medicine mixture, and fermenting raw materials to obtain raw material fermentation liquid;
step (3): and (3) drying the raw material fermentation broth to obtain the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus.
In the step (1), when the mixture A is mixed with water, the volume ratio of the mixture A to the water is 10:3.
In the step (1), 30 parts by weight of bran is added to the mixture A prepared and then mixed with water.
In step (1), the curing treatment method comprises the following steps: the mixture was steamed and heated to 80℃for 20min.
In the step (2), the probiotics are added into the traditional Chinese medicine mixture at the temperature of 30 ℃, the weight ratio of the probiotics to the traditional Chinese medicine mixture is 4:13, and raw material fermentation is carried out.
In the step (2), the temperature of raw material fermentation is 35 ℃, the time of raw material fermentation is 36 hours, and raw material fermentation liquid is obtained.
The preparation method of the probiotics comprises the following steps:
inoculating the enterococcus faecalis into a lactobacillus culture medium according to the inoculation amount of 0.5%, and performing heat preservation and activation for 2 hours at 30 ℃ to obtain an activation solution of the enterococcus faecalis;
inoculating the bacillus subtilis into a bacillus subtilis culture medium according to an inoculum size of 1%, and performing heat preservation and activation for 2 hours at 37 ℃ to obtain an activating solution of the bacillus subtilis;
inoculating the saccharomyces cerevisiae into a fungus culture medium according to an inoculum size of 0.2%, and performing heat preservation and activation for 2 hours at 30 ℃ to obtain an activating solution of the saccharomyces cerevisiae;
the probiotics are prepared by mixing 0.1 part of the prepared enterococcus faecalis activating solution, 0.2 part of bacillus subtilis activating solution and 0.2 part of saccharomyces cerevisiae activating solution according to parts by weight.
In step (3), drying the raw material fermentation broth comprises: the raw material fermentation broth was dried at 240 ℃ for 10s.
Example 3
A fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus, which comprises Chinese herbal medicines and probiotics;
the Chinese herbal medicine comprises the following components: astragalus root, pilose asiabell root, red paeony root, white paeony root and largehead atractylodes rhizome;
the probiotics include: bacillus subtilis, saccharomyces cerevisiae, enterococcus faecalis.
The Chinese herbal medicine comprises the following components in parts by weight: 23 parts of astragalus, 20 parts of codonopsis pilosula, 10 parts of red paeony root, 24 parts of white paeony root and 10 parts of largehead atractylodes rhizome; the probiotics comprise the following components in parts by weight: 0.1 part of bacillus subtilis, 0.2 part of saccharomyces cerevisiae and 0.2 part of enterococcus faecalis.
The preparation method of the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus comprises the following steps:
step (1): mixing the Chinese herbal medicines, crushing to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, and curing the mixed solution to obtain a Chinese herbal medicine mixture;
step (2): adding the probiotics into the traditional Chinese medicine mixture, and fermenting raw materials to obtain raw material fermentation liquid;
step (3): and (3) drying the raw material fermentation broth to obtain the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus.
In the step (1), when the mixture A is mixed with water, the volume ratio of the mixture A to the water is 10:3.
In the step (1), 10 parts by weight of rice flour is added to the mixture A prepared and then mixed with water.
In step (1), the curing treatment method comprises the following steps: the mixture was steamed and heated to 80℃for 20min.
In the step (2), the probiotics are added into the traditional Chinese medicine mixture at 35 ℃, the weight ratio of the probiotics to the traditional Chinese medicine mixture is 3:13, and raw material fermentation is carried out.
In the step (2), the temperature of raw material fermentation is 37 ℃, the time of raw material fermentation is 36 hours, and raw material fermentation liquid is obtained.
The preparation method of the probiotics comprises the following steps:
inoculating the enterococcus faecalis into a lactobacillus culture medium according to the inoculation amount of 0.5%, and performing heat preservation and activation for 2 hours at 35 ℃ to obtain an activation solution of the enterococcus faecalis;
inoculating the bacillus subtilis into a bacillus subtilis culture medium according to an inoculum size of 1%, and performing heat preservation and activation for 2 hours at 33 ℃ to obtain an activating solution of the bacillus subtilis;
inoculating the saccharomyces cerevisiae into a fungus culture medium according to an inoculum size of 0.2%, and performing heat preservation and activation for 2 hours at 26 to obtain an activating solution of the saccharomyces cerevisiae;
the probiotics are prepared by mixing 0.14 part of the prepared enterococcus faecalis activating solution, 0.12 part of bacillus subtilis activating solution and 0.1 part of saccharomyces cerevisiae activating solution in parts by weight.
In step (3), drying the raw material fermentation broth comprises: the raw material fermentation broth was dried at 244 ℃ for 10s.
The fermented traditional Chinese medicine preparation for resisting the porcine epidemic diarrhea virus prepared in the examples 1-3 is respectively subjected to a treatment test of the porcine epidemic diarrhea, and the specific operation is as follows:
the piglet in a certain scale farm has severe diarrhea, and the pathogen is confirmed to be porcine epidemic diarrhea virus according to clinical symptoms, pathological changes and laboratory diagnosis of the sick piglet. The 40 piglets with confirmed diagnosis are selected and randomly divided into 4 groups. Each group had 10 heads, which were blank, test 1 (example 1), test 2 (example 2), and test 3 (example 3). Each group was treated as follows:
blank group: feeding artificial milk;
test 1 group: feeding artificial milk +4kg/T the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus prepared in the example 1;
test 2 group: feeding artificial milk +4kg/T the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus prepared in the example 2;
test 3 group: feeding artificial milk +4kg/T the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus prepared in the example 3;
test results show that compared with the blank group, the cure rate of the test 1 group, the test 2 group and the test 3 group is improved, the death rate is reduced, the cure rate is between 60% and 80%, and the death rate is between 20% and 40%. Weight loss before and after the blank group piglet test was 0.41kg, and the test group piglet weight was increased by 0.07kg, 0.31kg and 0.07kg, respectively.
TABLE 1 influence of fermented Chinese medicinal preparation on piglet epidemic diarrhea
TABLE 2 variation of piglet growth performance
| Group of | Initial weight (kg/head) | Last weight (kg/head) |
| Blank group | 1.75±0.03 | 1.34±0.12 |
| Test 1 group | 1.78±0.04 | 1.85±0.11 |
| Test 2 groups | 1.75±0.02 | 2.06±0.21 |
| Test 3 groups | 1.76±0.02 | 1.83±0.16 |
The embodiment of the invention provides a traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus fermentation, probiotics in raw materials take Chinese herbal medicines as fermentation substrates, aromatic substances such as acetic acid, ethanol and the like are generated in the fermentation process, and the aromatic substances such as the ethanol and the like can reduce and eliminate bitter and astringent smell of the traditional Chinese medicine, so that the traditional Chinese medicine preparation is convenient for a live pig to eat. Under the fermentation action of probiotics, the cell wall of the traditional Chinese medicine plant can be promoted to be broken. The composition of radix astragali, radix Codonopsis, radix Paeoniae Rubra, radix Paeoniae alba and Atractylodis rhizoma can antagonize porcine epidemic diarrhea virus and treat porcine epidemic diarrhea.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (8)
1. The fermented traditional Chinese medicine preparation for resisting the porcine epidemic diarrhea virus is characterized by comprising Chinese herbal medicines and probiotics;
the Chinese herbal medicine consists of astragalus, dangshen, red paeony root, white paeony root and largehead atractylodes rhizome;
the probiotics consist of bacillus subtilis, saccharomyces cerevisiae and enterococcus faecalis;
the Chinese herbal medicine comprises the following components in parts by weight: 20-30 parts of astragalus, 10-20 parts of radix codonopsis, 10-15 parts of red paeony root, 20-25 parts of white paeony root and 10-25 parts of largehead atractylodes rhizome;
the probiotics comprise the following components in parts by weight: 0.1-0.2 part of bacillus subtilis, 0.1-0.2 part of saccharomyces cerevisiae and 0.1-0.2 part of enterococcus faecalis;
the method for preparing the fermented traditional Chinese medicine preparation for resisting the porcine epidemic diarrhea virus comprises the following steps:
step (1): mixing the Chinese herbal medicines, crushing to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, and curing the mixed solution to obtain a Chinese herbal medicine mixture;
step (2): adding the probiotics into the traditional Chinese medicine mixture, and fermenting raw materials to obtain raw material fermentation liquid;
step (3): and (3) drying the raw material fermentation broth to obtain the fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus.
2. The fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus according to claim 1, wherein in the step (1), when the mixture A is mixed with water, the volume ratio of the mixture A to the water is 10:3.
3. The fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus according to claim 1, wherein in the step (1), 10-30 parts by weight of any one of corn flour, bran or rice flour is added into the prepared mixture A, and then the mixture A is mixed with water.
4. The fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus according to claim 1, wherein in the step (1), the curing treatment method comprises the following steps: the mixture was steamed and heated to 80℃for 20min.
5. The fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus according to claim 1, wherein in the step (2), the probiotics is added into the traditional Chinese medicine mixture at 30-35 ℃, and the weight ratio of the probiotics to the traditional Chinese medicine mixture is (3-4): 13, and raw material fermentation is carried out.
6. The fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus according to claim 1, wherein in the step (2), the temperature of raw material fermentation is 35-37 ℃, and the time of raw material fermentation is 36 hours, so as to obtain raw material fermentation liquid.
7. The fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus according to claim 1, wherein the preparation method of the probiotics comprises the following steps:
inoculating the enterococcus faecalis into a lactobacillus culture medium according to the inoculation amount of 0.5%, and performing heat preservation and activation for 2 hours at 30+/-5 ℃ to obtain an activation solution of the enterococcus faecalis;
inoculating the bacillus subtilis into a bacillus subtilis culture medium according to an inoculum size of 1%, and performing heat preservation and activation for 2 hours at 30+/-5 ℃ to obtain an activating solution of the bacillus subtilis;
inoculating the saccharomyces cerevisiae into a fungus culture medium according to an inoculum size of 0.2%, and performing heat preservation and activation for 2 hours at 28+/-2 ℃ to obtain an activating solution of the saccharomyces cerevisiae;
the probiotics are prepared by mixing 0.1-0.2 part of the prepared enterococcus faecalis activating solution, 0.1-0.2 part of bacillus subtilis activating solution and 0.1-0.2 part of saccharomyces cerevisiae activating solution in parts by weight.
8. The fermented traditional Chinese medicine preparation for resisting porcine epidemic diarrhea virus according to claim 1, wherein in the step (3), the drying of the raw material fermentation broth comprises: drying the raw material fermentation broth at 240-260 ℃ for 10s.
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| CN106615808A (en) * | 2016-12-28 | 2017-05-10 | 梅州双胞胎饲料有限公司 | Pig feed additive capable of preventing and treating porcine respiratory syndromes and epidemic diarrhea |
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| CN114984114A (en) * | 2022-06-06 | 2022-09-02 | 湖北武当动物药业有限责任公司 | Liquid fermentation traditional Chinese medicine microecological preparation and application thereof in aspect of resisting porcine epidemic diarrhea virus |
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