CN111887350A - Fermented traditional Chinese medicine feed additive for promoting growth and improving resistance and preparation method thereof - Google Patents
Fermented traditional Chinese medicine feed additive for promoting growth and improving resistance and preparation method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- Animal Husbandry (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
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- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance, which is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight: 8-11 parts of astragalus membranaceus, 5-8 parts of angelica sinensis, 11-13 parts of codonopsis pilosula, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb. The invention also discloses a preparation method of the additive. The feed additive prepared by the invention is added into livestock and poultry feed, can obviously promote the growth of livestock and poultry, improve the daily gain, and improve the immunologic function and meat quality of the livestock and poultry.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance and a preparation method thereof.
Background
With the large-scale and intensive development of livestock and poultry breeding industry, antibiotics serving as feed additives promote the growth of animals and improve the body immunity of the animals are used in large quantities. The long-term use of the feed causes the drug resistance of pathogenic bacteria to be enhanced, and the human beings also can cause poisoning and other hazards after eating animal products with overproof antibiotic residues. Therefore, the search for new green, pollution-free feed additives has become a necessary trend.
The fermented traditional Chinese medicine has the advantages of no drug resistance, no drug residue, no pollution, various functions and the like, and becomes a hot point of research in recent years. The traditional Chinese medicine contains a plurality of active ingredients, has wide influence on the production performance and immunity of animals, and is a good substitute of antibiotics. The probiotics are nontoxic and have unique physiological effect, and can adjust the balance of intestinal flora, provide abundant small peptides, vitamins and other nutrients for organisms, and improve the oxidation resistance and immunity of the organisms. The fermentation of the traditional Chinese medicine organically combines the two, utilizes beneficial flora to ferment the traditional Chinese medicine, increases the release amount of active ingredients of the traditional Chinese medicine, has a large amount of active enzyme in fermentation liquor, can adjust immune function after being absorbed by organisms, enhances resistance, exerts the drug effect of the traditional Chinese medicine, and simultaneously can decompose toxic ingredients of the traditional Chinese medicine and reduce the damage of the medicine to animal organisms.
The problem of feed safety is widely concerned with the rapid development of the feed industry, and fermented traditional Chinese medicines with no public nuisance, pure nature, no antibiotic residue and comprehensive functions become a hotspot of research. The probiotic fermented traditional Chinese medicine has important influence on enhancing the curative effect, promoting the production performance of animals, reducing the feed cost, enhancing the immune function of organisms and the like, and has wide application prospect.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance and a preparation method thereof.
The invention is realized by the following technical scheme:
a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
8-11 parts of astragalus membranaceus, 5-8 parts of angelica sinensis, 11-13 parts of codonopsis pilosula, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb.
Preferably, the fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus, 6 parts of angelica, 12 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb.
Preferably, the microbial inoculum used for fermentation is a compound bacteria liquid, the compound bacteria liquid contains trichoderma, aspergillus niger and lactic acid bacteria, wherein the concentration of the trichoderma is 0.5-1.0 × 109CFU/mL, the concentration of black mold is 0.5-1.0 × 109CFU/mL, trichoderma concentration of 0.5-1.0 × 109CFU/mL。
The preparation method of the fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) weighing the traditional Chinese medicine raw materials according to the weight, chopping, uniformly mixing, and putting into a steam explosion tank for steam explosion treatment to obtain a pretreated mixture;
(2) crushing and sieving the pretreatment mixture to obtain pretreatment mixture powder;
(3) uniformly mixing the composite bacteria liquid, the fermentation culture medium and the pretreated mixture powder, and performing closed anaerobic fermentation at the temperature of 34-36 ℃ for 3-5 days to obtain a fermentation product;
(4) and (3) uniformly mixing the fermentation product with a proper amount of acetic acid and lipase, reacting for 2-4h at 38-45 ℃, and then drying to obtain the product.
Preferably, in the step (1), the pressure of the steam explosion treatment is 1-1.5MPa, and the time is 3-5 min.
Preferably, in the step (2), the particle size of the pre-treatment mixture powder is 80-100 meshes.
Preferably, in the step (3), the weight ratio of the composite bacteria liquid, the fermentation medium and the pretreated mixture powder is (0.05-0.07): (20-30): (10-15).
Preferably, the preparation method of the fermentation medium comprises the following steps: weighing 2.5-3.5g glucose, 8-12g peptone, 4-6g yeast extract, 1.5-2.5g K2HPO4、0.15-0.25g MgSO4·H2And O, adding a proper amount of water to dissolve, adjusting the pH value to 6.8-7.2, and then fixing the volume to 1000 mL.
Preferably, in the step (4), the ratio of the fermentation product, acetic acid and lipase is (30-50) g: (1-2) mL: (0.01-0.05) g, the lipase is at least one of Aspergillus niger lipase, Mucor lipase, Penicillium lipase and yeast lipase, and the enzyme activity is 5000-15000U/g.
Preferably, in the step (4), the drying temperature is 50-60 ℃.
The invention has the advantages that:
(1) the invention adopts a reasonable formula of traditional Chinese medicine raw materials such as astragalus, angelica, codonopsis pilosula, bighead atractylodes rhizome, Chinese yam, hawthorn, malt, rhizoma atractylodis and the like. Wherein, the astragalus can directly influence the metabolism in cells, induce the cells of the organism to generate related factors, enhance the specific and nonspecific immunity of the organism, play the role of immunoregulation in a multi-level way, increase the specific volume or the hemoglobin content of red blood cells and increase the number of the red blood cells; the codonopsis pilosula has the functions of tonifying vital qi, strengthening the immunity and disease resistance, and obviously strengthening the nonspecific immunity function, the humoral immunity function, the cellular immunity function and the interleukin; the fructus crataegi and fructus Hordei Germinatus have the effects of regulating qi-flowing, resolving food stagnation, invigorating spleen, and invigorating stomach. The palatability of the feed can be improved, the appetite of animals is improved, the conversion rate of the feed is improved, and the quality of animal products is improved; through reasonable compatibility of the traditional Chinese medicine raw materials, complementary effects of different action mechanisms of the traditional Chinese medicines can be exerted, daily gain can be improved, feed cost can be reduced, body resistance can be improved, the integral regulation function of the body can be enhanced, and accordingly growth of livestock and poultry can be effectively promoted and the body resistance can be enhanced.
(2) In the process for preparing the additive, the compound bacteria consisting of the reasonable compatibility of the trichoderma, the melanomyces and the lactic acid bacteria are adopted to ferment the traditional Chinese medicine raw materials, the compound bacteria can generate a plurality of enzymes and a plurality of secondary metabolites in the fermentation process, the products can enable the structure of the traditional Chinese medicine to generate hydroxylation or dehydrogenation reaction, so that the medicine property is changed, the medicine effect is improved, and a new treatment effect is generated, meanwhile, the traditional Chinese medicine and the microorganism effect are converted into components which are convenient to absorb and utilize by an organism, the micro-ecological environment of the organism is improved, the health care effect and the curative effect are improved, and the growth promoting capability and the disease resistance effect of the additive are.
(3) The invention creatively introduces the steam explosion pretreatment and the lipase esterification process on the basis of the traditional fermentation process to form a combined preparation process of steam explosion pretreatment-composite bacteria fermentation-lipase esterification, wherein the steam explosion pretreatment is firstly carried out, so that the internal structure of the traditional Chinese medicine raw materials is looser, the specific surface area is greatly improved, the number of active groups and reaction active sites are increased, the contact chance of reactants and a catalyst is increased, the composite bacteria fermentation can generate a large amount of active hydroxyl groups on the surfaces of the traditional Chinese medicine raw materials and release a large amount of active substances such as polysaccharide and the like in the raw materials, so that the active substances are easier to be absorbed and utilized by organisms, the antioxidation and growth promotion activity of the fermentation products is improved, the acetic acid and the lipase are added to carry out the biocatalytic esterification on the fermentation products, and the activity of the enzymatic esterification reaction is improved on the basis of the previous two steps, more medicinal material surface active hydroxyl and released polysaccharide active substances participate in esterification reaction, partial ester group is generated on the surface of the medicinal material, and partial acetic acid of the polysaccharide is esterified, so that the antioxidant activity and the immunocompetence of the polysaccharide active substances can be improved, partial hydroxyl can be converted into ester group, the stimulation of the medicinal material to the gastrointestinal tract is reduced, the absorption of the gastrointestinal tract is promoted, and the capabilities of promoting the growth and improving the immunity of the additive are better exerted.
In conclusion, the formula is selected and the preparation process is optimized, so that the prepared feed additive is added into the livestock feed, the growth of the livestock can be remarkably promoted, the daily gain is increased, and the immune function and the meat quality of the livestock are improved.
Detailed Description
In the following examples, the Lipase used was Aspergillus niger Lipase (Amano Lipase A), the enzyme activity was 12000U/g, CAS NO: 9001-62-1, available from Aldrich.
Example 1
A fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
8-11 parts of astragalus membranaceus, 5-8 parts of angelica sinensis, 11-13 parts of codonopsis pilosula, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb.
Example 2
A fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
8-11 parts of astragalus membranaceus, 5-8 parts of angelica sinensis, 11-13 parts of codonopsis pilosula, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb.
The microbial inoculum used for fermentation is a compound bacteria liquid, which contains trichoderma, black mold and lactic acid bacteria, wherein the concentration of the trichoderma is 0.5-1.0 × 109CFU/mL, the concentration of black mold is 0.5-1.0 × 109CFU/mL, trichoderma concentration of 0.5-1.0 × 109CFU/mL。
Example 3
A fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
9 parts of astragalus, 6 parts of angelica, 12 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb.
The microbial inoculum for fermentation is a compound bacteria liquid, which contains Trichoderma, Aspergillus niger and lactobacillus, wherein the concentration of Trichoderma is 0.8 × 109CFU/mL, the concentration of the black mold is 0.6X 109CFU/mL, Trichoderma concentration of 0.6X 109CFU/mL。
Example 4
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) weighing the following traditional Chinese medicine raw materials in parts by weight: 8 parts of astragalus membranaceus, 5 parts of angelica sinensis, 11 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 5 parts of Chinese yam, 6 parts of hawthorn, 10 parts of malt, 7 parts of rhizoma atractylodis, 9 parts of liquorice and 8 parts of cockscomb, chopping, uniformly mixing, putting into a steam explosion tank, and performing steam explosion for 5min under the condition that the pressure is 1MPa to obtain a pretreatment mixture;
(2) crushing and sieving the pre-treatment mixture to obtain pre-treatment mixture powder with the granularity of 80 meshes;
(3) mixing the composite bacteria liquid, the fermentation medium and the pretreated mixture powder according to the weight ratio of 0.05: 20: 10, mixing uniformly, and performing closed anaerobic fermentation at 34 ℃ for 5 days to obtain a fermentation product, wherein the concentration of the composite bacterial liquid is 0.5 multiplied by 109Trichoderma reesei of CFU/mL, concentration 0.5X 109CFU/mL of Aspergillus niger and concentration of 0.5X 109CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 2.5g glucose, 8g peptone, 4g yeast extract, 1.5g 1.5g K2HPO4、0.15g MgSO4·H2Adding a proper amount of water to dissolve O, adjusting the pH value to 6.8, and then fixing the volume to 1000mL to obtain a fermentation medium;
(4) mixing the fermentation product with acetic acid and lipase in a ratio of 30 g: 1mL of: 0.01g of the raw materials are uniformly mixed, reacted for 4 hours at the temperature of 38 ℃, and then dried at the temperature of 50 ℃ to obtain the catalyst.
Example 5
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) weighing the following traditional Chinese medicine raw materials in parts by weight: 11 parts of astragalus membranaceus, 8 parts of angelica sinensis, 13 parts of codonopsis pilosula, 9 parts of bighead atractylodes rhizome, 10 parts of Chinese yam, 10 parts of hawthorn, 12 parts of malt, 8 parts of rhizoma atractylodis, 13 parts of liquorice and 10 parts of cockscomb, chopping, uniformly mixing, putting into a steam explosion tank, and performing steam explosion for 3min under the condition that the pressure is 1.5MPa to obtain a pretreatment mixture;
(2) crushing and sieving the pre-treatment mixture to obtain pre-treatment mixture powder with the granularity of 100 meshes;
(3) mixing the composite bacteria liquid, the fermentation medium and the pretreated mixture powder according to a weight ratio of 0.07: 30: 15, mixing uniformly, and performing closed anaerobic fermentation at 36 ℃ for 3 days to obtain a fermentation product, wherein the concentration of the composite bacterial liquid is 1.0 multiplied by 109Trichoderma reesei (CFU/mL) at a concentration of 1.0X 109CFU/mL of Aspergillus niger and concentration of 1.0X 109CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing3.5g glucose, 12g peptone, 6g yeast extract, 2.5g K2HPO4、0.25g MgSO4·H2Adding a proper amount of water to dissolve O, adjusting the pH value to 7.2, and then fixing the volume to 1000mL to obtain a fermentation medium;
(4) mixing the fermentation product with acetic acid and lipase according to the proportion of 50 g: 2mL of: 0.05g of the raw materials are uniformly mixed, reacted for 2 hours at the temperature of 45 ℃, and then dried at the temperature of 60 ℃ to obtain the catalyst.
Example 6
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) weighing the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus membranaceus, 6 parts of angelica sinensis, 12 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb, chopping, uniformly mixing, putting into a steam explosion tank, and performing steam explosion for 4min under the condition that the pressure is 1.4MPa to obtain a pretreatment mixture;
(2) crushing and sieving the pre-treatment mixture to obtain pre-treatment mixture powder with the granularity of 90 meshes;
(3) mixing the composite bacteria liquid, the fermentation medium and the pretreated mixture powder according to a weight ratio of 0.06: 25: 12 mixing, performing closed anaerobic fermentation at 35 deg.C for 4 days to obtain fermented product, wherein the composite bacteria liquid contains bacteria with concentration of 0.8 × 109Trichoderma reesei of CFU/mL, concentration 0.6X 109CFU/mL of Aspergillus niger and concentration of 0.6X 109CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3g of glucose, 10g of peptone, 5g of yeast extract and 2g K2HPO4、0.2g MgSO4·H2Adding a proper amount of water to dissolve O, adjusting the pH value to 7, and then fixing the volume to 1000mL to obtain a fermentation medium;
(4) mixing the fermentation product with acetic acid and lipase according to the proportion of 40 g: 1.5 mL: 0.03g of the raw materials are uniformly mixed, reacted for 3 hours at 42 ℃, and then dried at 55 ℃ to obtain the catalyst.
Example 7
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) weighing the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus membranaceus, 6 parts of angelica sinensis, 12 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb, and after being uniformly mixed, the mixture is crushed and sieved to obtain mixture powder with the granularity of 90 meshes;
(2) mixing the composite bacteria liquid, the fermentation medium and the mixture powder according to the weight ratio of 0.06: 25: 12 mixing, performing closed anaerobic fermentation at 35 deg.C for 4 days to obtain fermented product, wherein the composite bacteria liquid contains bacteria with concentration of 0.8 × 109Trichoderma reesei of CFU/mL, concentration 0.6X 109CFU/mL of Aspergillus niger and concentration of 0.6X 109CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3g of glucose, 10g of peptone, 5g of yeast extract and 2g K2HPO4、0.2g MgSO4·H2Adding a proper amount of water to dissolve O, adjusting the pH value to 7, and then fixing the volume to 1000mL to obtain a fermentation medium;
(3) mixing the fermentation product with acetic acid and lipase according to the proportion of 40 g: 1.5 mL: 0.03g of the raw materials are uniformly mixed, reacted for 3 hours at 42 ℃, and then dried at 55 ℃ to obtain the catalyst.
Example 8
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) weighing the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus membranaceus, 6 parts of angelica sinensis, 12 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb, chopping, uniformly mixing, putting into a steam explosion tank, and performing steam explosion for 4min under the condition that the pressure is 1.4MPa to obtain a pretreatment mixture;
(2) crushing and sieving the pre-treatment mixture to obtain pre-treatment mixture powder with the granularity of 90 meshes;
(3) mixing the composite bacteria liquid, the fermentation medium and the pretreated mixture powder according to a weight ratio of 0.06: 25: 12 mixing, performing closed anaerobic fermentation at 35 deg.C for 4 days, and drying the obtained fermentation product at 55 deg.C to obtain the final product, wherein the composite bacteria liquid contains bacteria with concentration of 0.8 × 109Trichoderma reesei of CFU/mL, concentration 0.6X 109CFU/mL of Aspergillus niger and concentration of 0.6X 109CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3g of glucose, 10g of peptone, 5g of yeast extract and 2g of K2HPO4、0.2g MgSO4·H2And O, adding a proper amount of water to dissolve, adjusting the pH value to 7, and then metering the volume to 1000mL to obtain the fermentation medium.
Example 9
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) weighing the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus membranaceus, 6 parts of angelica sinensis, 12 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb, and after being uniformly mixed, the mixture is crushed and sieved to obtain mixture powder with the granularity of 90 meshes;
(2) mixing the composite bacteria liquid, the fermentation medium and the mixture powder according to the weight ratio of 0.06: 25: 12 mixing, performing closed anaerobic fermentation at 35 deg.C for 4 days, and drying the obtained fermentation product at 55 deg.C to obtain the final product, wherein the composite bacteria liquid contains bacteria with concentration of 0.8 × 109Trichoderma reesei of CFU/mL, concentration 0.6X 109CFU/mL of Aspergillus niger and concentration of 0.6X 109CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3g of glucose, 10g of peptone, 5g of yeast extract and 2g K2HPO4、0.2gMgSO4·H2And O, adding a proper amount of water to dissolve, adjusting the pH value to 7, and then metering the volume to 1000mL to obtain the fermentation medium.
Test examples
140 fattening pigs are randomly selected in a pig farm, the 140 fattening pigs are randomly divided into 7 groups, each group has 20 fattening pigs, the additive prepared in the embodiment 4-9 is added into basic feed according to 1% of the weight of the feed, after the additive is uniformly mixed, feeding tests are respectively carried out, a blank control group is fed with the basic feed, and the test period is 55 days. The initial weight and the end weight of the fattening pigs before and after each group of experiments are respectively recorded, and the immune indexes in the blood serum of the fattening pigs of each group after the experiments are measured, and the results are shown in tables 1 and 2.
TABLE 1 weight gain results before and after the feeding test
TABLE 2 immunization index after feeding test
| IgA(g/L) | IgG(g/L) | SOD(u/ml) | MDA(nmol/ml) | |
| Control group | 0.96 | 18.54 | 42 | 4.40 |
| Example 4 | 1.47 | 22.35 | 55 | 3.34 |
| Example 5 | 1.41 | 22.87 | 57 | 3.31 |
| Example 6 | 1.59 | 23.34 | 62 | 3.22 |
| Example 7 | 1.26 | 21.08 | 51 | 3.47 |
| Example 8 | 1.18 | 19.67 | 49 | 3.52 |
| Example 9 | 1.05 | 20.22 | 46 | 4.12 |
As can be seen from the results of the comparison of examples 4-9 with the control group, the daily weight gain of the pigs is higher than that of the control group when the additive of the invention is added into the feed; the IgA and IgG contents are increased compared with the control group, which shows that the anti-immunity capability of the organism can be improved; the content of SOD is increased, and the content of MDA is reduced, which shows that the antioxidant protection capability in the organism can be improved. Therefore, the feed additive can obviously improve the weight of the pigs, promote the growth and development of the pigs and improve the immunity of organisms, thereby increasing the economic benefit of breeders.
Through the comparative test results of the examples 6 to 9, it can be seen that the additive prepared by adopting the steam explosion pretreatment-complex enzyme fermentation-lipase esterification process in the example 6 can play more remarkable growth promoting and oxidation resisting roles compared with the additive prepared by adopting the complex enzyme fermentation-lipase esterification process only in the example 7, the steam explosion pretreatment-complex enzyme fermentation process only in the example 8 and the traditional complex enzyme fermentation process only in the example 9, and the synergistic effect among the processes of the additive preparation process is shown, so that the effects of promoting the growth and improving the immunity of the additive are better played.
Claims (10)
1. A fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is characterized by being prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
8-11 parts of astragalus membranaceus, 5-8 parts of angelica sinensis, 11-13 parts of codonopsis pilosula, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb.
2. The fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 1, wherein the additive is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus, 6 parts of angelica, 12 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb.
3. The fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 1 or 2, wherein the microbial inoculum for fermentation is a compound bacteria liquid, the compound bacteria liquid contains trichoderma, melanomyces and lactic acid bacteria, wherein the concentration of the trichoderma is 0.5-1.0 x 109CFU/mL, the concentration of black mold is 0.5-1.0 × 109CFU/mL, trichoderma concentration of 0.5-1.0 × 109CFU/mL。
4. A method for preparing a growth-promoting and resistance-enhancing fermented traditional Chinese medicine feed additive according to any one of claims 1 to 3, comprising the steps of:
(1) weighing the traditional Chinese medicine raw materials according to the weight, chopping, uniformly mixing, and putting into a steam explosion tank for steam explosion treatment to obtain a pretreated mixture;
(2) crushing and sieving the pretreatment mixture to obtain pretreatment mixture powder;
(3) uniformly mixing the composite bacteria liquid, the fermentation culture medium and the pretreated mixture powder, and performing closed anaerobic fermentation at the temperature of 34-36 ℃ for 3-5 days to obtain a fermentation product;
(4) and (3) uniformly mixing the fermentation product with a proper amount of acetic acid and lipase, reacting for 2-4h at 38-45 ℃, and then drying to obtain the product.
5. The method for preparing a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 4, wherein in the step (1), the pressure of the steam explosion treatment is 1-1.5MPa, and the time is 3-5 min.
6. The method for preparing a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 4, wherein in the step (2), the grain size of the pre-treatment mixture powder is 80-100 meshes.
7. The preparation method of the growth-promoting and resistance-improving fermented traditional Chinese medicine feed additive according to claim 4, wherein in the step (3), the weight ratio of the composite bacteria liquid, the fermentation medium and the pretreatment mixture powder is (0.05-0.07): (20-30): (10-15).
8. The method for preparing a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 4, wherein the method for preparing the fermentation culture medium comprises the following steps: weighing 2.5-3.5g glucose, 8-12g peptone, 4-6g yeast extract, 1.5-2.5g K2HPO4、0.15-0.25g MgSO4·H2And O, adding a proper amount of water to dissolve, adjusting the pH value to 6.8-7.2, and then fixing the volume to 1000 mL.
9. The method for preparing a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 4, wherein in the step (4), the ratio of the fermentation product, acetic acid and lipase is (30-50) g: (1-2) mL: (0.01-0.05) g, the lipase is at least one of Aspergillus niger lipase, Mucor lipase, Penicillium lipase and yeast lipase, and the enzyme activity is 5000-15000U/g.
10. The method for preparing a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 4, wherein in the step (4), the drying temperature is 50-60 ℃.
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| CN111388565A (en) * | 2020-05-25 | 2020-07-10 | 开封嘉骏生物科技有限公司 | Traditional Chinese medicine formula for improving hematopoiesis function of female livestock and young livestock and preparation method thereof |
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| CN108617853A (en) * | 2018-04-18 | 2018-10-09 | 佛山市椒图科技有限公司 | The feed addictive and preparation method for promoting chick growth, improving laying rate of laying hen |
| CN111388565A (en) * | 2020-05-25 | 2020-07-10 | 开封嘉骏生物科技有限公司 | Traditional Chinese medicine formula for improving hematopoiesis function of female livestock and young livestock and preparation method thereof |
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| CN113317393A (en) * | 2021-06-03 | 2021-08-31 | 焦作市佰役安生物工程有限公司 | Method for preparing fermentation type feed additive by using gas explosion |
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