CN107173587B - Compound feed containing selenium-rich dendrobium officinale probiotics and preparation method thereof - Google Patents

Compound feed containing selenium-rich dendrobium officinale probiotics and preparation method thereof Download PDF

Info

Publication number
CN107173587B
CN107173587B CN201710544990.9A CN201710544990A CN107173587B CN 107173587 B CN107173587 B CN 107173587B CN 201710544990 A CN201710544990 A CN 201710544990A CN 107173587 B CN107173587 B CN 107173587B
Authority
CN
China
Prior art keywords
dendrobium officinale
probiotic powder
selenium
ganoderma lucidum
hericium erinaceus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710544990.9A
Other languages
Chinese (zh)
Other versions
CN107173587A (en
Inventor
李海龙
何小平
戴承恩
张孟
郑芬芬
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ZHEJIANG YALIN BIOTECHNOLOGY CO Ltd
Original Assignee
ZHEJIANG YALIN BIOTECHNOLOGY CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ZHEJIANG YALIN BIOTECHNOLOGY CO Ltd filed Critical ZHEJIANG YALIN BIOTECHNOLOGY CO Ltd
Priority to CN201710544990.9A priority Critical patent/CN107173587B/en
Publication of CN107173587A publication Critical patent/CN107173587A/en
Application granted granted Critical
Publication of CN107173587B publication Critical patent/CN107173587B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention relates to the technical field of animal feed, and discloses a probiotic compound feed containing selenium-rich dendrobium officinale and a preparation method thereof. The compound feed comprises the following components in parts by weight: 50-70 parts of selenium-rich dendrobium officinale probiotic powder, 10-30 parts of ganoderma lucidum probiotic powder, 10-20 parts of hericium erinaceus probiotic powder and 5-10 parts of degreased pine pollen. Respectively sieving the selenium-rich dendrobium officinale probiotic powder, the ganoderma lucidum probiotic powder, the hericium erinaceus probiotic powder and the degreased pine pollen, and mixing in proportion to obtain the compound feed. The compound feed of the invention can replace antibiotics, can promote the growth of livestock and poultry, improve the digestion, absorption utilization rate and conversion rate of the feed, and increase the meat yield and the egg yield.

Description

Compound feed containing selenium-rich dendrobium officinale probiotics and preparation method thereof
Technical Field
The invention relates to the technical field of animal feed, and particularly relates to compound feed containing selenium-rich dendrobium officinale probiotics and a preparation method thereof.
Background
Probiotics is a kind of microorganism which is beneficial to a host by improving the microecological balance of the intestinal tract of the host, is important physiological bacteria of the intestinal tract of a human body, can promote the ecological balance of microbial flora in the intestinal tract, has important physiological effects of improving the structure of the intestinal tract flora, promoting the proliferation of beneficial bacteria in the intestinal tract, inhibiting the growth of harmful bacteria, enhancing the immunity of the organism, reducing cholesterol, eliminating carcinogenic factors, relieving lactose intolerance, preventing aging, prolonging life and the like, and has important significance for preventing and treating hypertension, hyperlipidemia, heart disease, diabetes and cancer.
Currently, many countries have begun to ban the use of antibiotics as animal growth promoters, however stopping the use of antibiotics in animal feed presents significant challenges to livestock and poultry farms. Antibiotic growth promoters have been reported to prevent necrotic enteritis in birds, and in some areas of disuse, increased incidence of necrotic enteritis in birds. Therefore, it has become necessary to reduce the use of a large amount of antibiotics, develop a microecological preparation which is both environmentally friendly and economically beneficial, and study a viable antibiotic substitute which enhances the innate immunity of animals.
In agriculture, probiotic preparations are mainly used to maintain the balance of the micro-ecosystem in animals, i.e. to reduce the loss of animal production by using probiotics in the case of young livestock not having established a good intestinal microflora; the microecological preparation can partially replace antibacterial drugs, achieves the aim of treating animal diseases by helping to restore the balance of the microecological system in the animal body, and has the advantages of safe use, no residue and no drug resistance.
Disclosure of Invention
The invention provides a natural and rich-nutrition compound feed capable of replacing antibiotics, and simultaneously solves the problem of recycling waste of residues left after extracts of Chinese herbal medicines such as dendrobium officinale residue, ganoderma lucidum residue, hericium erinaceus residue and the like.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a compound feed containing selenium-rich dendrobium officinale probiotics, which comprises the following components in parts by weight: 50-70 parts of selenium-rich dendrobium officinale probiotic powder, 10-30 parts of ganoderma lucidum probiotic powder, 10-20 parts of hericium erinaceus probiotic powder and 5-10 parts of degreased pine pollen;
the selenium-rich dendrobium officinale probiotic powder is a product obtained by fermenting bacillus coagulans and candida utilis with selenium-rich dendrobium officinale as raw materials, the ganoderma lucidum probiotic powder is a product obtained by fermenting cellulomonas and bezoar ruminococcus with ganoderma lucidum as raw materials, and the hericium erinaceus probiotic powder is a product obtained by fermenting pediococcus acidilactici with hericium erinaceus as raw materials.
Preferably, the selenium-rich dendrobium officinale probiotic powder is prepared by the following steps: adding bran extract accounting for 0.5-1% of the total weight of the dendrobium officinale and sodium selenite accounting for 0.005-0.01% of the total weight of the dendrobium officinale into the dendrobium officinale, then inoculating bacillus coagulans seed liquid accounting for 5-10% of the total weight of the dendrobium officinale and candida utilis seed liquid accounting for 2-5% of the total weight of the dendrobium officinale, controlling the fermentation temperature to be 38-40 ℃, and fermenting for 50-70 hours.
Preferably, the content of organic selenium in the selenium-rich dendrobium officinale probiotic powder is more than 4 mg/kg.
Preferably, the bran extract is oat bran extract.
Preferably, the ganoderma lucidum probiotic powder is prepared by the following steps: inoculating cellulomonas seed liquid accounting for 5-8% of the total weight of the ganoderma lucidum and rumen coccus bovis seed liquid accounting for 2-4% of the total weight of the ganoderma lucidum into the ganoderma lucidum, controlling the fermentation temperature to be 28-30 ℃, and fermenting for 60-80 h.
Preferably, the hericium erinaceus probiotic powder is prepared by the following steps: adding fructo-oligosaccharide 0.5% of the total weight of the hericium erinaceus into the hericium erinaceus, then inoculating pediococcus acidilactici seed liquid 8% of the total weight of the hericium erinaceus, controlling the fermentation temperature at 37 ℃, and fermenting for 12-36 hours.
Preferably, the defatted pine pollen is prepared by using supercritical CO2The technology comprises the steps of extracting for 0.5-3 h at an extraction pressure of 25-45 MPa and an extraction temperature of 30-50 ℃ and CO2Extracting fat-soluble components from the pine pollen under the condition of the flow rate of 20-40L/h.
The invention also comprises a preparation method of the selenium-rich dendrobium officinale probiotic compound feed, wherein the selenium-rich dendrobium officinale probiotic powder, the ganoderma lucidum probiotic powder, the hericium erinaceus probiotic powder and the defatted pine pollen are respectively sieved and mixed in proportion to obtain the compound feed.
Preferably, the sieving size of the selenium-rich dendrobium officinale probiotic powder and the ganoderma lucidum probiotic powder is 20-50 meshes.
Compared with the prior art, the invention has the following advantages:
the compound feed provided by the invention can further utilize waste dendrobium officinale residue, ganoderma lucidum residue and hericium erinaceus residue, and also provides a natural, non-toxic and side-effect, safe, reliable, multifunctional, residue-free and environment-friendly feed for animals. The compound feed of the invention can replace antibiotics, not only overcomes the adverse effect of the antibiotics on other microbial communities in the digestive tract, but also avoids the toxic and side effects and drug resistance generated by the long-term use of the antibiotics, can promote the growth of livestock and poultry, improves the digestion, absorption and utilization rate and the conversion rate of the feed, and increases the meat yield and the egg yield.
The probiotic strains involved in the invention are all safe strains approved by the national health commission and applicable to food and feed, have a long history in safety application, are considered to be symbiotic microorganisms without pathogenic possibility, and are mostly separated from intestinal tracts of healthy animals. The functions of the probiotic compound feed are mainly embodied in the following aspects: (1) the polysaccharides such as dendrobium officinale, hericium erinaceus and ganoderma lucidum can improve the macrophage activity and immunoglobulin level of animals and reduce the occurrence of animal diseases and plagues; (2) amylase, protease and lipase are secreted to promote digestion and absorption of nutrient substances; (3) complex carbohydrates which cannot be digested by a host in the feed, such as pectin, glucan, cellulose, xylose and the like, are degraded, and the feed utilization rate is improved; (4) can be metabolized to generate a plurality of vitamins in physical ability, such as B vitamins like Bl, B2, B6 and the like and vitamin C, and nicotinic acid, folic acid and the like provide vitamin nutrition for animals, thereby promoting the improvement of the production performance of the animals; (5) lactic acid is generated to promote animals to absorb mineral salts such as iron, phosphorus, calcium and the like and fat-soluble nutrient substances such as vitamin D and the like; (6) can inhibit putrefying bacteria for producing amine, reduce pH value in intestinal tract, produce amino oxidase and enzymes for decomposing hydrogen sulfide, and oxidize indoles into nontoxic and harmless substances, thereby reducing the concentration and odor of ammonia gas and hydrogen sulfide in livestock and poultry houses, and reducing environmental pollution.
Detailed Description
The invention provides a compound feed containing selenium-rich dendrobium officinale probiotics, which comprises the following components in parts by weight: 50-70 parts of selenium-rich dendrobium officinale probiotic powder, 10-30 parts of ganoderma lucidum probiotic powder, 10-20 parts of hericium erinaceus probiotic powder and 5-10 parts of degreased pine pollen;
the selenium-rich dendrobium officinale probiotic powder is a product obtained by fermenting bacillus coagulans and candida utilis with selenium-rich dendrobium officinale as raw materials, the ganoderma lucidum probiotic powder is a product obtained by fermenting cellulomonas and bezoar ruminococcus with ganoderma lucidum as raw materials, and the hericium erinaceus probiotic powder is a product obtained by fermenting pediococcus acidilactici with hericium erinaceus as raw materials.
In the compound feed, the addition amount of the selenium-rich dendrobium officinale probiotic powder is 50-70 parts by weight, preferably 53-67 parts by weight, and more preferably 56-63 parts by weight. The probiotics in the selenium-enriched dendrobium officinale probiotic powder are bacillus coagulans and candida utilis, the dendrobium officinale is fermented by the probiotics, inorganic selenium sodium selenite is added in the fermentation process, the inorganic selenium is converted into organic selenium by the probiotics, and the content of the organic selenium in the fermentation product is increased. The invention has no special limitation on the source of the dendrobium officinale, and adopts the dendrobium officinale medicinal materials well known by the technical personnel in the field or uses the extracted dendrobium officinale slag. In the specific embodiment of the invention, the dendrobium officinale slag is preferably used, and the dendrobium officinale slag is waste obtained by extracting dendrobium officinale with water, oscillating and filtering. Further preferably, the dendrobium officinale slag is waste of the dendrobium officinale subjected to water extraction twice.
Specifically, the selenium-rich dendrobium officinale probiotic powder disclosed by the invention is preferably prepared by the following steps: adding bran extract and sodium selenite into herba Dendrobii, inoculating Bacillus coagulans and Candida utilis, fermenting, drying and pulverizing the fermented product.
In the invention, the bran extract is oat bran extract, preferably, the addition amount of the bran extract is 0.5-1%, more preferably 0.7-0.9% of the total weight of the dendrobium officinale, the bran extract is an excellent organic nitrogen source, contains more than 20% of amino acid, and also contains thiamine, riboflavin, nicotinic acid and other growth factors necessary for the growth of microorganisms, and experiments prove that after 5% of the bran extract is added into the dendrobium officinale, the spore concentration of bacillus coagulans after fermentation culture reaches 1.2 × 1010CFU/g, 3.5 times higher than the control group without bran extract. The bran extract provided by the invention can be beneficial to the bacillus coagulans to produce spores, and the spore production rate of the bacillus coagulans is more than 90%.
According to the invention, sodium selenite is added into the dendrobium officinale, and inorganic selenium and amino acid are combined through biotransformation to form nontoxic organic selenium which is easy to absorb and utilize. In the invention, the addition amount of the sodium selenite is 0.005-0.01% of the weight of the dendrobium officinale residue, and more preferably 0.006-0.008%. The content of organic selenium in the selenium-rich dendrobium officinale probiotic powder obtained after fermentation is more than 4 mg/kg.
The probiotics added into the dendrobium officinale slag are bacillus coagulans and candida utilis, wherein the bacillus coagulans mainly has two functions of 1 secreting enzyme substances such as cellulase and amylase and further decomposing cellulose in the dendrobium officinale into substances such as utilizable polysaccharide and 2 secreting antibacterial peptides and other substances to inhibit harmful bacteria such as enterococcus, salmonella and escherichia coli in animals and simultaneously producing lactic acid to reduce the pH value of intestinal tracts, and the candida utilis can ferment to produce substances such as cell polysaccharide and extracellular polysaccharide to improve the immunity of the animals and simultaneously convert the cellulose into substances such as protein and amino acid7CFU/ml, more preferably (1 to 3) × 107CFU/ml. In the invention, the inoculation amount of the seed solution of the bacillus coagulans is 5-10% of the total weight of the dendrobium officinale, and preferably 6-8%. The inoculation amount of the candida utilis seed liquid is 2-5% of the total weight of the dendrobium officinale, and more preferably 3-4%.
When the dendrobium officinale is fermented, the fermentation temperature is controlled to be 38-40 ℃, and the preferable temperature is 39 ℃. The fermentation process is anaerobic fermentation without introducing air. Fermenting for 50-70 h, and further, finishing the fermentation after 56-64 h.
Drying and crushing the fermented product to obtain the selenium-rich dendrobium officinale probiotic powder. The drying and pulverizing method of the present invention is not particularly limited, and a drying and pulverizing method known to those skilled in the art is used. In a particular embodiment of the invention, vacuum drying is preferably employed. The degree of vacuum for vacuum drying is preferably 4 to 8Kpa, and more preferably 5 to 7 Kpa. The temperature of vacuum drying is controlled to be 50-55 ℃, and more preferably 52-54 ℃. During vacuum drying, the thickness of the material is preferably 2-3 cm, and the drying time is preferably 3-5 h. And (3) crushing the vacuum-dried material, preferably controlling the crushing granularity to be 30 meshes, and obtaining the selenium-rich dendrobium officinale probiotic powder, wherein the content of organic selenium is more than 4 mg/kg.
The composite feed contains the ganoderma lucidum probiotic powder, and the addition amount of the ganoderma lucidum probiotic powder is 10-30 parts by weight, preferably 17-26 parts by weight, and more preferably 19-24 parts by weight. The probiotics added in the ganoderma lucidum probiotic powder are cellulomonas and bezoar ruminococcus, and the ganoderma lucidum is fermented by the probiotics to obtain the ganoderma lucidum probiotic powder. The source of the ganoderma lucidum is not particularly limited, and the ganoderma lucidum medicinal materials known by the technical personnel in the field or the extracted ganoderma lucidum fruiting body residues are adopted. In the embodiment of the invention, the ganoderma lucidum dregs are preferably used, the ganoderma lucidum dregs are waste obtained by extracting, oscillating and filtering ganoderma lucidum blocks with ethanol, the ethanol is preferably 70% ethanol, and the extraction frequency of the ganoderma lucidum dregs is preferably 2 times.
The ganoderma lucidum probiotic powder is prepared by preferably inoculating cellulomonas sobria and ruminococcus bovis into ganoderma lucidum, fermenting, and drying and crushing a fermentation product to obtain the cellulolytic enzyme, wherein the cellulolytic enzyme can be generated by both the cellulolytic enzyme and the ruminococcus bovis, and can decompose cellulose in the ganoderma lucidum into substances which are digested and absorbed by digestion, and meanwhile, the utilization rate of feed is promoted in an animal body7CFU/ml, more preferably (1 to 3) × 107CFU/ml. In the invention, the inoculation amount of the seed liquid of the cellulomonas is 5-8% of the total weight of the ganoderma lucidum, and further 6-7%. The inoculation amount of the rumen cow-bezoar rumen coccus seed solution is 2-4% of the total weight of the ganoderma lucidum, and is more preferably 3%.
When the ganoderma lucidum is fermented, the fermentation temperature is controlled to be 28-30 ℃, and the preferred fermentation temperature is 29 ℃. The fermentation process is anaerobic fermentation. Fermenting for 60-80 h, and further finishing fermentation after 64-72 h.
Drying and crushing the fermented product to obtain the ganoderma lucidum probiotic powder. The drying and pulverizing method of the present invention is not particularly limited, and a drying and pulverizing method known to those skilled in the art is used. In a particular embodiment of the invention, vacuum drying is preferably employed. The degree of vacuum for vacuum drying is preferably 4 to 8Kpa, and more preferably 5 to 7 Kpa. The temperature of vacuum drying is controlled to be 50-55 ℃, and more preferably 52-54 ℃. During vacuum drying, the thickness of the material is preferably 2-3 cm, and the drying time is preferably 3-5 h. And (3) crushing the vacuum-dried material, preferably controlling the crushing granularity to be 30 meshes, and obtaining the ganoderma lucidum probiotic powder.
The compound feed also contains hericium erinaceus probiotic powder, and the addition amount of the hericium erinaceus probiotic powder is 10-20 parts, preferably 13-18 parts, and more preferably 15-17 parts. The probiotic added into the hericium erinaceus probiotic powder is pediococcus acidilactici, and beneficial substances contained in the hericium erinaceus are utilized to ferment the pediococcus acidilactici to obtain the hericium erinaceus probiotic powder which is rich in protein, bacteriocin and the like and has the function of improving immunity. The source of the hericium erinaceus is not particularly limited, and hericium erinaceus medicinal materials well known by persons skilled in the art or extracted hericium erinaceus residues are adopted. In the specific embodiment of the invention, the hericium erinaceus residues are preferably used, and are wastes obtained by water extraction, oscillation and filtration of hericium erinaceus. The water extraction frequency of the Hericium erinaceus residue is preferably 2 times.
The hericium erinaceus probiotic powder is prepared by the following steps of adding fructo-oligosaccharide into hericium erinaceus, inoculating pediococcus acidilactici, fermenting, drying and crushing a fermentation product, wherein the pediococcus acidilactici can generate antibacterial substances such as organic acid and bacteriocin in the fermentation process, can maintain the microecological balance of animal intestinal tracts, and can promote absorption and utilization of substances such as protein and monosaccharide, the inoculated probiotics are preferred to be seed liquid of the probiotics, and the concentration of the pediococcus acidilactici in the seed liquid is (1-5) × 107CFU/ml, more preferably (1 to 3) × 107CFU/ml. In the invention, the inoculation amount of the seed liquid of pediococcus acidilactici is 8-10% of the total weight of the hericium erinaceus, and further 9%. To be beneficial to the occurrence of pediococcus acidilacticiIn the fermentation process, proliferation and bacteriocin generation are carried out, and fructo-oligosaccharide is added into hericium erinaceus for fermentation. The addition amount of the fructo-oligosaccharide is preferably 0.5-1% of the total weight of the hericium erinaceus, and more preferably 0.6-0.8%.
When the hericium erinaceus is fermented, the fermentation temperature is controlled to be 35-37 ℃, and preferably 36 ℃. The fermentation process is anaerobic fermentation. Fermenting for 12-36 h, and further finishing the fermentation after 24 h.
And drying and crushing the fermented product to obtain the hericium erinaceus probiotic powder. The drying and pulverizing method of the present invention is not particularly limited, and a drying and pulverizing method known to those skilled in the art is used. In a particular embodiment of the invention, vacuum drying is preferably employed. The degree of vacuum for vacuum drying is preferably 4 to 8Kpa, and more preferably 5 to 7 Kpa. The temperature of vacuum drying is controlled to be 50-55 ℃, and more preferably 52-54 ℃. During vacuum drying, the thickness of the material is preferably 2-3 cm, and the drying time is preferably 3-5 h. And (3) crushing the vacuum-dried material, preferably controlling the crushing granularity to be 30 meshes, and obtaining the hericium erinaceus probiotic powder.
The composite feed also contains 5-10 parts by weight of defatted pine pollen, preferably 6-9 parts by weight, and more preferably 7-8 parts by weight. The defatted pine pollen is obtained by extracting fat-soluble components in the pine pollen, can be obtained by adopting the commercially available defatted pine pollen or can be obtained by automatically defatting the pine pollen. In the present invention, supercritical CO is preferably used2The fat-soluble components in the pine pollen are extracted by the technology. Wherein, supercritical CO2The technical extraction conditions are as follows: the extraction pressure is 25-45 MPa, preferably 35-40 MPa; the extraction temperature is 30-50 ℃, and preferably 35-45 ℃; the extraction time is 0.5-3 h, preferably 1-2 h. CO extraction2The flow rate of (A) is 20 to 40L/h, more preferably 25 to 35L/h. The pine pollen is rich in nutrient components, contains more than 20 kinds of amino acids, 14 kinds of vitamins, more than 30 kinds of mineral elements, about 10% of fat, nearly one hundred kinds of enzymes and coenzymes, nucleic acid, flavone, organic acid, glycoside and the like, and is called as 'complete nutrient powder'. The defatted pollen Pini is rich in protein and amino acidsThe feed additive can provide more comprehensive nutrition for animals, improve the immunity of the animals and promote the generation and development of various stages.
The invention also comprises a preparation method of the compound feed containing the selenium-rich dendrobium officinale probiotics. Sieving the selenium-rich dendrobium officinale probiotic powder, the ganoderma lucidum probiotic powder, the hericium erinaceus probiotic powder and the defatted pine pollen prepared by the method respectively, and mixing the sieved powder and the powder in proportion to obtain the compound feed. Preferably, the materials are sieved by a sieve with 20-50 meshes, and more preferably a sieve with 30 meshes. The mixing device and the mixing mode are not particularly limited in the invention, and the materials can be mixed by adopting the mixing device and the mixing mode which are well known to the technical personnel in the field. In the embodiment of the invention, the compound feed is obtained by uniformly mixing the components in a three-dimensional mixer.
The compound feed and the basic ration are mixed to feed animals, preferably, the mixing ratio of the compound feed and the basic ration is 20-80 g/kg, and more preferably 30-60 g/kg. The adding proportion can be properly adjusted according to the types of different animals so as to achieve the effects of promoting the growth of livestock and poultry, improving the digestion, absorption and utilization rate and conversion rate of the feed and increasing the meat yield and the egg yield.
The present invention will be described in detail with reference to examples for better understanding the objects, technical solutions and advantages of the present invention, but they should not be construed as limiting the scope of the present invention.
Example 1
The invention relates to a preparation method of a selenium-rich dendrobium officinale probiotic compound feed, which comprises the following components in percentage by mass: 70% of selenium-rich dendrobium officinale probiotic powder, 10% of ganoderma lucidum probiotic powder, 10% of hericium erinaceus probiotic powder and 10% of defatted pine pollen.
The selenium-rich dendrobium officinale probiotic powder is prepared by adding bran extract accounting for 0.5 percent of the total mass of a solid culture medium and sodium selenite accounting for 0.005 percent of the total mass of dendrobium officinale dregs into the dendrobium officinale dregs, and then inoculating bacillus coagulans seed solution accounting for 5 percent of the total mass of the solid culture medium and candida utilis seed solution accounting for 5 percent of the total mass of the solid culture medium, wherein the concentration of the seed solution of the strain is 1 × 107CFU/ml. Control hairThe fermentation temperature is 38 ℃, the fermentation is finished for 75h, and then the product is obtained by vacuum drying and crushing, the selenium content is detected to be 4.58mg/kg, and the spore yield of the bacillus coagulans is 92.4%.
The ganoderma lucidum probiotic powder is prepared by inoculating cellomonas seed liquid accounting for 8% of the total mass of a solid culture medium and bezoar ruminococcus seed liquid accounting for 4% of the total mass of the solid culture medium into ganoderma lucidum residues, wherein the concentration of the seed liquid is 1 × 107CFU/ml. Controlling the fermentation temperature to be 28 ℃, finishing the fermentation for 72 hours, and then drying and crushing in vacuum to obtain the product.
The hericium erinaceus probiotic powder is prepared by adding fructo-oligosaccharide 0.5% of the total mass of a solid culture medium into hericium erinaceus residues, and then inoculating pediococcus acidilactici seed liquid 8% of the total mass of the solid culture medium, wherein the concentration of the seed liquid is 1 × 107CFU/ml. Controlling the fermentation temperature at 37 ℃, finishing the fermentation for 30h, and then drying and crushing in vacuum to obtain the product.
The defatted pollen Pini is prepared by supercritical CO2The technology comprises the steps of extracting for 1h at an extraction pressure of 35MPa and an extraction temperature of 40 ℃ and CO2Extracting liposoluble components from pollen Pini at flow rate of 30L/h.
Respectively sieving selenium-rich dendrobium officinale probiotic powder, lucid ganoderma probiotic powder, hericium erinaceus probiotic and defatted pine pollen with a 30-mesh sieve, respectively weighing the processed raw materials according to the formula proportion, and putting the raw materials into a three-dimensional mixer for uniformly mixing; and (4) subpackaging the uniformly mixed materials by a granule packaging machine, and obtaining the product after the product is qualified through product inspection.
Example 2:
the invention relates to a preparation method of a selenium-rich dendrobium officinale probiotic compound feed, which comprises the following components in percentage by mass: 60% of selenium-rich dendrobium officinale probiotic powder, 20% of ganoderma lucidum probiotic powder, 15% of hericium erinaceus probiotic powder and 5% of defatted pine pollen.
The selenium-rich dendrobium officinale probiotic powder is prepared by the following steps: adding bran extract 0.8% of total mass of solid culture medium and sodium selenite 0.01% into the Dendrobium officinale residue, inoculating Bacillus coagulans seed solution 8% of total mass of solid culture medium and Candida utilis seed solution 2%, and collecting the seed solutionIs 2 × 107CFU/ml. Controlling the fermentation temperature to be 39 ℃, finishing the fermentation for 54h, and then drying and crushing in vacuum to obtain the selenium-enriched bacillus coagulans product, wherein the detected selenium content is 5.19mg/kg, and the spore production rate of the bacillus coagulans is 94.5%;
the ganoderma lucidum probiotic powder is prepared by inoculating 5% of Cellulomonas cellulosa seed liquid and 2% of ruminococcus bovis seed liquid in the total mass of a solid culture medium into ganoderma lucidum residue, wherein the concentration of the seed liquid is 2.5 × 107CFU/ml. Controlling the fermentation temperature to be 30 ℃, finishing the fermentation for 69h, and then drying and crushing in vacuum to obtain the product;
the hericium erinaceus probiotic powder is prepared by adding fructo-oligosaccharide accounting for 1% of the total mass of a solid culture medium into hericium erinaceus residues, and then inoculating pediococcus acidilactici seed liquid accounting for 8% of the total mass of the solid culture medium, wherein the concentration of the seed liquid is 1.5 × 107CFU/ml. Controlling the fermentation temperature to 35 ℃, finishing the fermentation for 20h, and then drying and crushing in vacuum to obtain the product;
the defatted pollen Pini is prepared by supercritical CO2The technology comprises the steps of extracting for 1h at the extraction pressure of 32MPa and the extraction temperature of 38 ℃ and CO2Extracting liposoluble components from pollen Pini at flow rate of 25L/h.
Respectively sieving selenium-rich dendrobium officinale probiotic powder, lucid ganoderma probiotic powder, hericium erinaceus probiotic and defatted pine pollen with a 40-mesh sieve, respectively weighing the processed raw materials according to the formula proportion, and putting the raw materials into a three-dimensional mixer for uniformly mixing; and (4) subpackaging the uniformly mixed materials by a granule packaging machine, and obtaining the product after the product is qualified through product inspection.
Example 3:
the invention relates to a preparation method of a selenium-rich dendrobium officinale probiotic compound feed, which comprises the following components in percentage by mass: 50% of selenium-rich dendrobium officinale probiotic powder, 30% of ganoderma lucidum probiotic powder, 12% of hericium erinaceus probiotic powder and 8% of defatted pine pollen.
The selenium-rich dendrobium officinale probiotic powder is prepared by the following steps: adding bran extract 1% of the total mass of solid culture medium and sodium selenite 0.008% into the Dendrobium officinale residue, and inoculating Bacillus coagulans seed solution 6% of the total mass of solid culture medium and Candida utilis 3%The concentration of the yeast seed liquid is 3 × 107CFU/ml. Controlling the fermentation temperature to be 40 ℃, finishing the fermentation for 60h, and then drying and crushing in vacuum to obtain the selenium-enriched bacillus coagulans product, wherein the selenium content is 4.32mg/kg and the spore production rate of the bacillus coagulans is 92.7 percent.
The ganoderma lucidum probiotic powder is prepared by inoculating 6 percent of cellomonas seed liquid and 3 percent of bezoar ruminococcus seed liquid by the total mass of a solid culture medium into ganoderma lucidum slag, wherein the concentration of the seed liquid is 2.7 × 107CFU/ml. Controlling the fermentation temperature to be 29 ℃, finishing the fermentation for 76h, and then drying and crushing in vacuum to obtain the product.
The hericium erinaceus probiotic powder is prepared by adding fructo-oligosaccharide 0.7% of the total mass of a solid culture medium into hericium erinaceus residues, and then inoculating pediococcus acidilactici seed liquid 6% of the total mass of the solid culture medium, wherein the concentration of the seed liquid is 3.7 × 107CFU/ml. Controlling the fermentation temperature to be 36 ℃, finishing the fermentation for 24h, and then drying and crushing in vacuum to obtain the product.
The defatted pollen Pini is prepared by supercritical CO2The technology comprises the steps of extracting for 1h at the extraction pressure of 34MPa and the extraction temperature of 39 ℃ and CO2Extracting liposoluble components from pollen Pini at flow rate of 28L/h.
Respectively sieving selenium-rich dendrobium officinale probiotic powder, lucid ganoderma probiotic powder, hericium erinaceus probiotic and defatted pine pollen with a 30-mesh sieve, respectively weighing the processed raw materials according to the formula proportion, and putting the raw materials into a three-dimensional mixer for uniformly mixing; and (4) subpackaging the uniformly mixed materials by a granule packaging machine, and obtaining the product after the product is qualified through product inspection.
Example 4:
the piglet is fed with the probiotic compound feed of the invention:
the average weight of the piglets is about 5 kg. 50g/kg of the probiotic compound feed is added into the basal ration of the piglet, and after the piglet is fed for one month, the average weight of the piglet fed with the probiotic compound feed is 27.5kg, and the average weight of the piglet fed with the basal ration is 18.5 kg. Compared with piglets fed with basic ration only, the feed conversion ratio of the piglets fed with the probiotic feed provided by the invention is reduced by 15.3%, the diarrhea rate is reduced by 85.5%, the average daily gain is 32.7%, and the death rate is reduced by 7.68%.
Example 5:
the laying hens feed the probiotic compound feed of the invention:
30g/kg of the probiotic compound feed is added into basic daily ration of the laying hens, the laying rate is increased by 31.1%, the egg breakage rate is reduced by 6.18%, the death rate is reduced by 5.69%, and the average feed-egg ratio is increased by 12.5%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (5)

1. The compound feed containing the selenium-rich dendrobium officinale probiotics is characterized by comprising the following components in parts by weight: 50-70 parts of selenium-rich dendrobium officinale probiotic powder, 10-30 parts of ganoderma lucidum probiotic powder, 10-20 parts of hericium erinaceus probiotic powder and 5-10 parts of degreased pine pollen;
the selenium-rich dendrobium officinale probiotic powder is a product obtained by fermenting bacillus coagulans and candida utilis with selenium-rich dendrobium officinale as raw materials, the ganoderma lucidum probiotic powder is a product obtained by fermenting cellulomonas and bezoar ruminococcus with ganoderma lucidum as raw materials, and the hericium erinaceus probiotic powder is a product obtained by fermenting pediococcus acidilactici with hericium erinaceus as raw materials;
the selenium-rich dendrobium officinale probiotic powder is prepared by the following steps: adding a bran extract accounting for 0.5-1% of the total weight of the dendrobium officinale and 0.005-0.01% of sodium selenite into the dendrobium officinale, then inoculating a bacillus coagulans seed solution accounting for 5-10% of the total weight of the dendrobium officinale and a candida utilis seed solution accounting for 2-5% of the total weight of the dendrobium officinale, controlling the fermentation temperature to be 38-40 ℃, and fermenting for 50-70 hours;
the ganoderma lucidum probiotic powder is prepared by the following steps: inoculating cellulomonas seed liquid accounting for 5-8% of the total weight of the ganoderma lucidum and rumen coccus bovis seed liquid accounting for 2-4% of the total weight of the ganoderma lucidum into the ganoderma lucidum, controlling the fermentation temperature to be 28-30 ℃, and fermenting for 60-80 h;
the hericium erinaceus probiotic powder is prepared by the following steps: adding fructo-oligosaccharide 0.5% of the total weight of the hericium erinaceus into the hericium erinaceus, then inoculating pediococcus acidilactici seed liquid 8% of the total weight of the hericium erinaceus, controlling the fermentation temperature at 37 ℃, and fermenting for 12-36 hours;
the defatted pollen Pini is prepared by supercritical CO2The technology comprises the steps of extracting for 0.5-3 h at an extraction pressure of 25-45 MPa and an extraction temperature of 30-50 ℃ and CO2Extracting fat-soluble components from the pine pollen under the condition of the flow rate of 20-40L/h.
2. The compound feed as claimed in claim 1, wherein the selenium-rich dendrobium officinale probiotic powder contains organic selenium of more than 4 mg/kg.
3. The compound feed according to claim 1, characterized in that the bran extract is oat bran extract.
4. A method for preparing the compound feed as claimed in any one of claims 1 to 3, comprising the steps of: sieving the selenium-rich dendrobium officinale probiotic powder, the ganoderma lucidum probiotic powder, the hericium erinaceus probiotic powder and the degreased pine pollen respectively, and mixing in proportion to obtain the compound feed.
5. The method according to claim 4, wherein the size of the sieve is 20 to 50 meshes.
CN201710544990.9A 2017-07-06 2017-07-06 Compound feed containing selenium-rich dendrobium officinale probiotics and preparation method thereof Active CN107173587B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710544990.9A CN107173587B (en) 2017-07-06 2017-07-06 Compound feed containing selenium-rich dendrobium officinale probiotics and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710544990.9A CN107173587B (en) 2017-07-06 2017-07-06 Compound feed containing selenium-rich dendrobium officinale probiotics and preparation method thereof

Publications (2)

Publication Number Publication Date
CN107173587A CN107173587A (en) 2017-09-19
CN107173587B true CN107173587B (en) 2020-08-28

Family

ID=59844641

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710544990.9A Active CN107173587B (en) 2017-07-06 2017-07-06 Compound feed containing selenium-rich dendrobium officinale probiotics and preparation method thereof

Country Status (1)

Country Link
CN (1) CN107173587B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ743958A (en) 2016-01-07 2023-07-28 Native Microbials Inc Methods for improving milk production by administration of microbial consortia
AU2018260547A1 (en) 2017-04-28 2019-10-10 Native Microbials, Inc. Methods for supporting grain intensive and/or energy intensive diets in ruminants with a synthetic bioensemble of microbes
CN111202753A (en) * 2020-02-19 2020-05-29 湖南加农正和生物技术有限公司 Medicine composition for preventing and treating piglet infection and improving immunity and swine fever antibody and using method
US20230139325A1 (en) * 2020-03-31 2023-05-04 Native Microbials, Inc. Improving milk production and compositional characteristics with novel ruminococcus bovis
CN115997933A (en) * 2022-06-23 2023-04-25 苏州聚和堂生物科技有限公司 Yolk globulin probiotic powder and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1307092A (en) * 2000-01-25 2001-08-08 云南省林业科学院 CO2 supercritical extraction of fat compounds from pollen pini
CN1709133A (en) * 2005-07-12 2005-12-21 南京农业大学 Selenium-rich probiotics foder additive production method and its product
CN104293716A (en) * 2014-10-08 2015-01-21 湖南民康生物技术研究所 Method for preparing efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm)

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1307092A (en) * 2000-01-25 2001-08-08 云南省林业科学院 CO2 supercritical extraction of fat compounds from pollen pini
CN1709133A (en) * 2005-07-12 2005-12-21 南京农业大学 Selenium-rich probiotics foder additive production method and its product
CN104293716A (en) * 2014-10-08 2015-01-21 湖南民康生物技术研究所 Method for preparing efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
乳酸菌素在饲料中应用研究;金银卡编辑部;《广东饲料》;20161231;第25卷(第12期);第32-34页 *

Also Published As

Publication number Publication date
CN107173587A (en) 2017-09-19

Similar Documents

Publication Publication Date Title
CN107173587B (en) Compound feed containing selenium-rich dendrobium officinale probiotics and preparation method thereof
CN102805209B (en) Synbiotics feed additive for beasts and birds and application thereof
CN105724772A (en) Chinese herbal medicine micro-ecological compound feed additive and preparation and use method thereof
EP2859797A1 (en) Additive composition for shrimp feed and a method for producing the same
CN111011643A (en) Bacterium enzyme fermented traditional Chinese medicine for improving growth performance of broiler chicken and preparation method thereof
CN106260504A (en) A kind of method utilizing beer yeast slurry to produce fermentable moist forage
CN101999555B (en) Animal nutrient special for sheep and method for preparing the same
CN112136965B (en) Immunity-enhancing and growth-promoting fermented Chinese herbal medicine feed additive and preparation method thereof
CN103609858A (en) Feed additive and preparation method thereof
CN110892939A (en) Method for producing baicalein by fermenting bacillus subtilis, feed additive prepared by fermentation and preparation method
CN110946907A (en) Traditional Chinese medicine composition for young poultry, liquid fermented traditional Chinese medicine preparation and preparation method thereof
CN111011602A (en) Functional feed additive for relieving oxidative stress of livestock and poultry and improving intestinal health and preparation method thereof
CN105053566A (en) Cornus walteri seed proteinase feed additive and preparation method thereof
CN112806498A (en) Laying hen tea residue bacterium enzyme synergistic fermentation feed and preparation method thereof
CN101999556B (en) Special animal nutrient for beef cattle and preparation method thereof
CN111296723A (en) Probiotic agent for multi-strain combined solid state fermentation of rapeseed meal and fermentation method thereof
CN106234799A (en) A kind of piglet fermentation of Chinese herbal medicine feed additive and preparation technology thereof
CN111887350B (en) Fermented traditional Chinese medicine feed additive capable of promoting growth and improving resistance and preparation method thereof
CN103495136A (en) Fermented Chinese herbal medicine for treating livestock gastrointestinal tract parasitic disease and preparation method of fermented Chinese herbal medicine
CN112568335A (en) Compound feed for enhancing goose physique
CN110870511A (en) Dry-wet mixed concentrated feed and preparation method thereof
CN110178989A (en) A kind of Soybean Peptide feed making method of high efficiency, low cost
KR101034467B1 (en) Microorganism cultivating medium containing enzymatic hydrolysate of wheat bran and konjak
CN104982673A (en) Preparation method of probiotic fermentation chicken feed additive
CN112715777A (en) Feed for broiler chickens and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of invention: A compound feed containing selenium enriched Dendrobium candidum probiotics and its preparation method

Effective date of registration: 20221122

Granted publication date: 20200828

Pledgee: Zhejiang Tailong Commercial Bank Co.,Ltd. Hangzhou Fuyang sub branch

Pledgor: ZHEJIANG YALIN BIOTECHNOLOGY Co.,Ltd.

Registration number: Y2022980022630