CN110178989A - A kind of Soybean Peptide feed making method of high efficiency, low cost - Google Patents
A kind of Soybean Peptide feed making method of high efficiency, low cost Download PDFInfo
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- CN110178989A CN110178989A CN201910419081.1A CN201910419081A CN110178989A CN 110178989 A CN110178989 A CN 110178989A CN 201910419081 A CN201910419081 A CN 201910419081A CN 110178989 A CN110178989 A CN 110178989A
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- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 50
- 244000068988 Glycine max Species 0.000 title claims abstract description 48
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 100
- 230000004151 fermentation Effects 0.000 claims abstract description 92
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 239000000203 mixture Substances 0.000 claims abstract description 41
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 32
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 239000004365 Protease Substances 0.000 claims abstract description 25
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 20
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 19
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 18
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 18
- 102000004190 Enzymes Human genes 0.000 claims abstract description 17
- 229940088598 enzyme Drugs 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 108091005508 Acid proteases Proteins 0.000 claims abstract description 14
- 108090000526 Papain Proteins 0.000 claims abstract description 11
- 235000019834 papain Nutrition 0.000 claims abstract description 11
- 229940055729 papain Drugs 0.000 claims abstract description 11
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 10
- 235000000346 sugar Nutrition 0.000 claims abstract description 10
- 108010004032 Bromelains Proteins 0.000 claims abstract description 8
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 8
- 235000019835 bromelain Nutrition 0.000 claims abstract description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract 3
- 238000010564 aerobic fermentation Methods 0.000 claims description 26
- 239000001963 growth medium Substances 0.000 claims description 26
- 239000002054 inoculum Substances 0.000 claims description 23
- 239000002609 medium Substances 0.000 claims description 20
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- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 19
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 19
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
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- 230000000694 effects Effects 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 9
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 9
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 4
- 244000099147 Ananas comosus Species 0.000 claims description 2
- 235000007119 Ananas comosus Nutrition 0.000 claims description 2
- 101800000263 Acidic protein Proteins 0.000 claims 1
- 241000186605 Lactobacillus paracasei Species 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 8
- 235000019629 palatability Nutrition 0.000 abstract description 2
- 230000001131 transforming effect Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 6
- 241000235342 Saccharomycetes Species 0.000 description 6
- 239000000047 product Substances 0.000 description 6
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- 239000002994 raw material Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
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- 235000019710 soybean protein Nutrition 0.000 description 5
- 244000199866 Lactobacillus casei Species 0.000 description 4
- 235000013958 Lactobacillus casei Nutrition 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
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- 244000300264 Spinacia oleracea Species 0.000 description 3
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- 238000007747 plating Methods 0.000 description 3
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- 238000012545 processing Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 238000011218 seed culture Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 235000015099 wheat brans Nutrition 0.000 description 3
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 2
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 2
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- 244000189799 Asimina triloba Species 0.000 description 1
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- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000006053 animal diet Substances 0.000 description 1
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- 235000006708 antioxidants Nutrition 0.000 description 1
- 241001615895 bacterium IS6 Species 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
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- 239000003205 fragrance Substances 0.000 description 1
- 230000000774 hypoallergenic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
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- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
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- 229910052700 potassium Inorganic materials 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention belongs to biological feedstuff fields, are related to a kind of Soybean Peptide feed making method of high efficiency, low cost；Step are as follows: activated strains respectively obtain bacillus licheniformis, candida utili and lactobacillus-fermented seed liquor；Papain, bromelain, brown sugar, potassium dihydrogen phosphate and epsom salt are added in bean dregs, it is mixed to get a phase fermentation medium, it is inoculated with the lichen bacillus ferments seed liquor and candida utili fermentation seed liquid carries out a phase bacterium enzyme cooperative fermentation, obtain a phase fermenting mixture；Lactobacillus-fermented seed liquor is inoculated, acid protease is added, is mixed to get the second stage of fermenting mixture；The second stage of anaerobic fermentation is carried out under air-proof condition, obtains soybean peptide feed after anaerobic fermentation；The present invention is using the mode of bacterium enzyme cooperative fermentation and aerobic anaerobism step fermentation, and thoroughly, soybean peptide transforming degree is high, high financial profit for fermentation；Palatability is good, and animal is fond of, and verifies through Animals fed experimental, can significantly improve the weight of animal.
Description
Technical field
The invention belongs to biological feedstuff fields, and in particular to a kind of Soybean Peptide feed processing side of high efficiency, low cost
Method.
Background technique
Soybean Peptide is soy proteinaceous hydrolysate, is to be converted soybean protein by microbial fermentation technology
At the mixture of polypeptide, oligopeptides, small peptide and small percentage of amino acids.Its relative molecular weight is generally 5000Da or less.With soybean egg
White to compare, Soybean Peptide is more easy to digest and absorbs, and exempts from anti-oxidant, hypoallergenic, the absorption of promotion mineral element, enhancing
The functions such as epidemic disease function.Soybean protein rich in bean dregs, and it is cheap, it is a kind of fabulous feeding to be developed utilized
Expect raw material.Produced using microbial fermentation technology fermented soybean dregs high quality soybean peptide feed be it is a kind of have huge economic value with
The effective ways of Practical significance.
It is mostly produced greatly with raw materials such as soybean, dregs of beans, soy meal, soybean protein isolates using fermentation method or enzymatic isolation method at present
Beans peptide product, seldom using this cheap waste of bean dregs as raw material.As 1,500,881 101988080 A of A, CN of patent CN,
1827772 A of CN is respectively using soybean, soybean protein isolate, dregs of beans as raw material, and patent CN 1500881 A, CN
101988080 A are in processed soybeans, soybean protein isolate, it is also necessary to processing, the higher cost such as are heat-treated, be centrifuged.
For another example patent CN 1461597 A, CN 102178028 A, CN 102048022 A also need to be concentrated in vacuo it is dry or spraying dry
Dry equal processing, the high requirements on the equipment and is not easy large-scale production.In addition to this, also have using microwave evaporation technology and produce soybean
Peptide product；If 107873949 A of patent CN produces soybean peptide product using microwave evaporation technical treatment soybean and soya-bean milk,
For the soybean peptide that the method obtains without bitter taste, raw material availability is high, but its is cumbersome, time-consuming and laborious.For at present, existing report
The research method in road the problems such as there is complex production process, higher cost.
Summary of the invention
In view of the problems of the existing technology, present invention seek to address that one of described problem, provides a kind of high efficiency, low cost
Soybean Peptide feed making method.This method is easy to operate, and significant effect, cost is extremely low, remarkable in economical benefits, acquisition
Soybean peptide feed quality is good, is rich in probiotics, and have sour taste, can partially make an addition in animal diets.
In order to achieve the goal above, the technical solution adopted by the present invention is specific as follows:
(1) bacillus licheniformis, candida utili and lactic acid bacteria are inoculated into fluid nutrient medium respectively and carry out activation training
It supports, obtains the lichen bacillus ferments seed liquor, candida utili fermentation seed liquid and lactobacillus-fermented seed liquor；
(2) it configures a phase fermentation medium: adding appropriate papain, bromelain, brown sugar, phosphoric acid in bean dregs
Potassium dihydrogen (KH2PO4) and epsom salt (MgSO4·7H2O it) and is uniformly mixed, obtains a phase fermentation medium；
(3) inoculation step (1) obtains the lichen bacillus ferments seed liquor and candida utili fermentation seed liquid in
In the phase fermentation medium that step (2) obtains, water content is adjusted, fermentation temperature carries out a phase bacterium enzyme cooperative fermentation, fermentation one
A phase fermenting mixture is obtained after fixing time；
(4) the lactobacillus-fermented seed liquor that inoculation step (1) obtains is in the phase fermenting mixture that step (3) obtains,
Appropriate acid protease is added, is uniformly mixed, obtains the second stage of fermenting mixture；The second stage of anaerobism hair is carried out under air-proof condition
Ferment obtains soybean peptide feed after anaerobic fermentation.
Preferably, each component weight percentage of a phase aerobic fermentation culture medium described in step (2) is respectively as follows: bean dregs
97%-99.7%, brown sugar 0.1%-0.7%, KH2PO40.05%-0.4%, MgSO4·7H2O 0.01%-0.5%；Pawpaw egg
The total addition level of white enzyme and bromelain is 0.05%-1.8%.
Preferably, the enzyme activity of papain described in step (2) is 200,000 u/g, and the enzyme activity of bromelain is
300000 u/g.
Preferably, the lichen bacillus ferments seed liquor described in step (3) and candida utili fermentation seed liquid
Inoculum concentration is the 0.1%-8% of a phase fermentation medium weight；The viable bacteria of the bacillus licheniformis and candida utili
Sum is 1.5 × 109-5×109cfu/mL。
Preferably, adjusting water content described in step (3) is 35%-75%.
Preferably, the temperature of fermentation described in step (3) is 20-37 DEG C, time 6-12h.
Preferably, lactobacillus-fermented seed liquor described in step (4) includes lactobacillus acidophilus fermentation seed liquid, rhamnose cream
Bacillus fermentation seed liquor, Lactobacillus casei fermentation seed liquid and lactobacillus plantarum fermentation seed liquid；The lactobacillus-fermented seed
The total viable count of liquid is 2 × 109-9×109Cfu/mL, additive amount are the 0.2%-12% of fermenting mixture weight.
Preferably, lactobacillus-fermented seed liquor described in step (4) is lactobacillus plantarum fermentation seed liquid and acidophilus cream bar
When bacterium fermentation seed liquid, the inoculum concentration ratio of two kinds of bacterium is 1:1；The lactobacillus-fermented seed liquor is Lactobacillus rhamnosus fermentation
When seed liquor and lactobacillus plantarum fermentation seed liquid, the inoculum concentration ratio of two kinds of bacterium is 1:4.
Preferably, the additive amount of acid protease described in step (4) is the 0.01%- of a phase fermenting mixture weight
0.5%；The enzyme activity of the acid protease is 60,000 u/g.
Preferably, the water content of second phase fermenting mixture described in step (4) is 40%-80%.
Preferably, the time of the fermentation of second phase described in step (4) is 3-9 days, and temperature is 20-37 DEG C.
Wherein, acid protease is purchased from Cangzhou Xia Sheng biotechnology of enzymes Co., Ltd, papain and bromelain
It is purchased from the Nanning road Dong Henghua biotechnology Co., Ltd, is common commercial enzyme preparation, is commercially available in the market.
Strain source: lactobacillus plantarum (Lactobacillus plantarum) CGMCC 1.557, lactobacillus acidophilus
(Lactobacillus acidophilus) CGMCC 1.2467, Lactobacillus rhamnosus (Lactobacillus rhamnosus)
CGMCC 1.577, Lactobacillus casei (Lactobacillus casei) CGMCC 1.8727, bacillus licheniformis (Bacillus
Licheniformis)) CGMCC1.813, candida utili (Candida utilis) CGMCC2.1180, above-mentioned strain are equal
It is purchased from China General Microbiological culture presevation administrative center.
The beneficial effects of the present invention are:
(1) the method for the present invention uses this cheap bean product of bean dregs for fermentation raw material, at low cost, and does not need large size and set
Standby investment, method are simple and practical.
(2) present invention is using the mode of bacterium enzyme cooperative fermentation and aerobic anaerobism step fermentation, and fermentation is thorough, soybean peptide
Transforming degree is high, and soybean peptide content is high, significant effect.
(3) present invention uses a variety of probiotics, and fermented product is rich in a variety of probiotics viable bacterias, has to the intestinal health of animal
There is Beneficial Effect.
(4) the product acid fragrance that the method for the present invention obtains assails the nostrils, and no bitter taste, palatability is good, and animal is fond of；Through animal feeding
Experimental verification can significantly improve the weight of animal.
Detailed description of the invention
Fig. 1 is the general flowchart of zymotechnique of the present invention.
Specific embodiment:
The invention will be further described combined with specific embodiments below.
Embodiment 1:
(1) activation culture of bacillus licheniformis, candida utili and lactic acid bacteria:
A, the culture medium of bacillus licheniformis, candida utili and lactic acid bacteria is configured first；
Bacillus inclined-plane and plating medium: yeast extract 5g, sodium chloride 10g, peptone 10g, agar 15g, glucose
5g, distilled water 1L, pH are naturally, 121 DEG C of sterilizing 30min；The same slant medium of ingredient of bacillus liquid seed culture medium, area
It is not that agar is not added；
Saccharomycete inclined-plane and plating medium: malt extract powder 130g, chloramphenicol 0.1g, agar 15g, 1000mL distilled water,
PH6.0 ± 0.2,115 DEG C of sterilizing 15min.The same slant medium of the ingredient of saccharomycete liquid seed culture medium, difference are that fine jade is not added
Rouge；
Lactic acid bacteria inclined-plane and plating medium: yeast extract 5g, sodium chloride 10g, peptone 10g, agar 20g, glucose
15g, distilled water 1L, pH are natural.110 DEG C of sterilizing 30min.The same slant medium of the ingredient of lactic acid bacteria liquid seed culture medium, area
It is not that agar is not added；
B, the activation culture method of bacillus licheniformis, candida utili and lactic acid bacteria:
Bacillus liquid Spawn incubation: the bacillus that 4 DEG C save is seeded to inclined-plane culture respectively under aseptic condition
Base, 37 DEG C are cultivated recovery strain for 24 hours, then bacterium colony is drawn on plate, and picking seed is inoculated into respectively equipped with 100mL fluid nutrient medium
Shaking flask (250mL) in, 170r/min, 37 DEG C of culture 20h obtain fermentation of bacillus seed liquor, according to actual needs again with 1%
Inoculum concentration be inoculated into a large amount of fluid nutrient mediums expand culture；The bacillus is bacillus licheniformis CGMCC1.813；
Saccharomycete strain cultivation: being seeded to slant medium for the saccharomycete that 4 DEG C save respectively under aseptic condition, and 28
DEG C culture 30h recovery strain, then on plate draw bacterium colony, the strain after picking culture be inoculated into respectively equipped with 120mL liquid train
In the shaking flask (250mL) for supporting base, 160r/min, 28 DEG C of culture 20h obtain saccharomycetes to make fermentation seed liquor, and according to actual needs again 3%
Inoculum concentration be inoculated into a large amount of fluid nutrient mediums expand culture；The saccharomycete is candida utili CGMCC2.1180；
Lactic acid bacteria cultural method: the lactobacillus inoculum for respectively saving 4 DEG C under aseptic condition to slant medium, 30 DEG C of trainings
30h recovery strain is supported, then draws bacterium colony on plate, picking seed is inoculated into the shaking flask equipped with 100mL fluid nutrient medium respectively
In (250mL), 150r/min, 30 DEG C of cultures for 24 hours, obtain lactobacillus-fermented seed liquor, according to actual needs again with 1% inoculum concentration
It is inoculated into a large amount of fluid nutrient mediums and expands culture；The lactic acid bacteria includes lactobacillus acidophilus CGMCC 1.2467, rhamnose cream
Bacillus CGMCC 1.577, Lactobacillus casei CGMCC 1.8727 and lactobacillus plantarum CGMCC 1.557.
(2) a phase solid fermentation basal medium is configured, each component weight percentage is respectively as follows: bean dregs 99.27%；Wood
Melon proteinase-10 .05%；Bromelain 0.3%；Brown sugar 0.25%；KH2PO40.05%；MgSO4·7H2O 0.08%, mixing
Uniformly, a phase fermentation medium is obtained；
(3) by the lichen bacillus ferments seed liquor and candida utili fermentation seed after step (1) activation culture
Liquid is inoculated into a phase aerobic fermentation culture medium, and inoculum concentration is the 5% of a phase aerobic fermentation culture medium weight, bacillus licheniformis
Total viable count with candida utili is 5 × 109Cfu/mL, the water content for adjusting culture medium is 70%, and fermentation temperature is
33 DEG C, aerobic fermentation 6h, a phase fermenting mixture is obtained after carrying out a phase aerobic fermentation；
(4) the phase fermenting mixture that inoculated plant lactobacillus ferment seed liquor is obtained in step (3), inoculum concentration are a phase
The 6% of fermenting mixture weight, the total viable count of lactobacillus plantarum fermentation seed liquid are 9 × 109Cfu/mL, and add acid egg
White enzyme, the additive amount of acid protease are the 0.01% of a phase fermenting mixture weight；It is uniformly mixed, adjustment water content is
80%, obtain the second stage of fermenting mixture；It is placed in unidirectional venthole fermentation bag and carries out the second stage of anaerobic fermentation, hair under air-proof condition
Ferment temperature is 30 DEG C, obtains soybean peptide feed after fermentation 7 days.
Embodiment 2:
(1) activation culture of bacillus licheniformis, candida utili, lactobacillus plantarum and lactobacillus acidophilus is specific to grasp
Make with embodiment 1；
(2) a phase solid fermentation basal medium is configured, each component weight percentage is respectively as follows: bean dregs 98.13%；Spinach
The additive amount of trailing plants protease and papain is 0.6%；Brown sugar 0.5%；KH2PO40.08%；MgSO4·7H2O
0.09%, it is uniformly mixed, obtains a phase aerobic fermentation culture medium；
(3) by the lichen bacillus ferments seed liquor and candida utili fermentation seed after step (1) activation culture
Liquid is inoculated into a phase aerobic fermentation culture medium, and inoculum concentration is the 8% of a phase aerobic fermentation culture medium weight, bacillus licheniformis
Total viable count with candida utili is 1.5 × 109Cfu/mL, the water content for adjusting culture medium is 70%, fermentation temperature
It is 30 DEG C, aerobic fermentation 12h, obtains a phase fermenting mixture after carrying out a phase aerobic fermentation；
(4) phase that inoculated plant lactobacillus ferment seed liquor and lactobacillus acidophilus fermentation seed liquid are obtained in step (3)
Fermenting mixture, total inoculum concentrations of two kinds of bacterium are the 10% of a phase fermenting mixture weight, the total viable count of two kinds of bacterium is 2 ×
109The inoculum concentration ratio of cfu/mL, lactobacillus plantarum fermentation seed liquid and lactobacillus acidophilus fermentation seed liquid is 1:1, and is added
Acid protease, the additive amount of acid protease are the 0.5% of a phase fermenting mixture weight；It is uniformly mixed, adjusts water content
It is 80%, obtains the second stage of fermenting mixture；It is placed in unidirectional venthole fermentation bag and carries out the second stage of anaerobic fermentation under air-proof condition,
Fermentation temperature is 33 DEG C, obtains soybean peptide feed after fermentation 5 days.
Embodiment 3:
(1) activation culture of bacillus licheniformis, candida utili, Lactobacillus rhamnosus and lactobacillus plantarum, specifically
Operation is the same as embodiment 1；
(2) a phase solid fermentation basal medium is configured, each component weight percentage is respectively as follows: bean dregs 98.94%；Spinach
The additive amount of trailing plants protease and papain is 0.15%；Brown sugar 0.7%；KH2PO40.05%；MgSO4·7H2O
0.01%, it is uniformly mixed, obtains a phase aerobic fermentation culture medium；
(3) by the lichen bacillus ferments seed liquor and candida utili fermentation seed after step (1) activation culture
Liquid is inoculated into a phase aerobic fermentation culture medium, and inoculum concentration is the 3% of a phase aerobic fermentation culture medium weight, bacillus licheniformis
Total viable count with candida utili is 1.5 × 109Cfu/mL, the water content for adjusting culture medium is 70%, fermentation temperature
It is 30 DEG C, aerobic fermentation 10h, obtains a phase fermenting mixture after carrying out a phase aerobic fermentation；
(4) it is inoculated with Lactobacillus rhamnosus fermentation seed liquid and lactobacillus plantarum fermentation seed liquid are obtained in step (3) one
Phase fermenting mixture, total inoculum concentrations of two kinds of bacterium are the 12% of a phase fermenting mixture weight, the viable count of two kinds of bacterium is 6 ×
109The inoculum concentration ratio of cfu/mL, Lactobacillus rhamnosus fermentation seed liquid and lactobacillus plantarum fermentation seed liquid is 1:4, and is added
Enter acid protease, the additive amount of acid protease is the 0.08% of a phase fermenting mixture weight；It is uniformly mixed, adjusts aqueous
Amount is 80%, obtains the second stage of fermenting mixture；It is placed in unidirectional venthole fermentation bag and carries out the second stage of anaerobism hair under air-proof condition
Ferment, fermentation temperature are 33 DEG C, obtain soybean peptide feed after fermentation 4 days.
Embodiment 4:
(1) activation culture of bacillus licheniformis, candida utili and lactobacillus plantarum, concrete operations are the same as embodiment 1；
(2) a phase solid fermentation basal medium is configured, each component weight percentage is respectively as follows: bean dregs 97%；Pineapple
The additive amount of protease and papain is 0.9%；Brown sugar 0.3%；KH2PO40.4%；MgSO4·7H2O 0.5% is mixed
It closes uniformly, obtains a phase aerobic fermentation culture medium；
(3) by the lichen bacillus ferments seed liquor and candida utili fermentation seed after step (1) activation culture
Liquid is inoculated into a phase aerobic fermentation culture medium, and inoculum concentration is the 0.1% of a phase aerobic fermentation culture medium weight, lichens gemma bar
The total viable count of bacterium and candida utili is 5 × 109Cfu/mL, the water content for adjusting culture medium is 35%, fermentation temperature
It is 37 DEG C, aerobic fermentation 6h, obtains a phase fermenting mixture after carrying out a phase aerobic fermentation；
(4) the phase fermenting mixture that inoculated plant lactobacillus ferment seed liquor is obtained in step (3), total inoculum concentration are one
The 0.2% of phase fermenting mixture weight, the total viable count of lactobacillus plantarum fermentation seed liquid are 9 × 109Cfu/mL, and acid is added
Property protease, the additive amount of acid protease is the 0.01% of a phase fermenting mixture weight；It is uniformly mixed, adjustment water content is
40%, obtain the second stage of fermenting mixture；It is placed in unidirectional venthole fermentation bag and carries out the second stage of anaerobic fermentation, hair under air-proof condition
Ferment temperature is 37 DEG C, obtains soybean peptide feed after fermentation 3 days.
Embodiment 5:
(1) activation culture of bacillus licheniformis, candida utili and lactobacillus plantarum, concrete operations are the same as embodiment 1；
(2) a phase solid fermentation basal medium is configured, each component weight percentage is respectively as follows: bean dregs 99.7%；Spinach
The additive amount of trailing plants protease and papain is 0.025%；Brown sugar 0.1%；KH2PO40.05%；MgSO4·7H2O
0.1%, it is uniformly mixed, obtains a phase aerobic fermentation culture medium；
(3) by the lichen bacillus ferments seed liquor and candida utili fermentation seed after step (1) activation culture
Liquid is inoculated into a phase aerobic fermentation culture medium, and inoculum concentration is the 8% of a phase aerobic fermentation culture medium weight, bacillus licheniformis
Total viable count with candida utili is 3.5 × 109Cfu/mL, the water content for adjusting culture medium is 75%, fermentation temperature
It is 20 DEG C, aerobic fermentation 12h, obtains a phase fermenting mixture after carrying out a phase aerobic fermentation；
(4) the phase fermenting mixture that inoculated plant lactobacillus ferment seed liquor is obtained in step (3), inoculum concentration are a phase
The 12% of fermenting mixture weight, the total viable count of lactobacillus plantarum fermentation seed liquid are 2 × 109Cfu/mL, and the acid being added
Property protease, the additive amount of acid protease is the 0.5% of a phase fermenting mixture weight；It is uniformly mixed, adjustment water content is
80%, obtain the second stage of fermenting mixture；It is placed in unidirectional venthole fermentation bag and carries out the second stage of anaerobic fermentation, hair under air-proof condition
Ferment temperature is 20 DEG C, obtains soybean peptide feed after fermentation 9 days.
Table 1 is the soybean peptide content of soybean peptide finished feed in embodiment
| Detection project | Testing result |
| Soybean peptide content (example 1) | 60.12% |
| Soybean peptide content (example 2) | 69.80% |
| Soybean peptide content (example 3) | 62.30% |
| Soybean peptide content (example 4) | 58.10% |
| Soybean peptide content (example 5) | 64.03% |
Embodiment 6: administering transgenic
Testing site is the Zhejiang Jiaxing South Lake town Yu Xin star's animal husbandry pig farm, and microorganism used therefor feed is prepared by embodiment 2
Soybean peptide feed.20 child care pigs (Landrace) are taken, 20 kilograms or so, random combine at two groups, every group 20, match by test daily ration
Fang Rubiao 2 is raised 90 days, test result such as table 3.
Feed cost valence of the invention is 2000 yuan/ton, and price is 2500 yuan per ton, so with the 20% micro- life of the present invention
Object feed replaces 20% wheat bran, and wheat bran presses 1500 yuan of calculations per ton, 37 yuan more than the test group feed cost in diet feed cost.
But drop to 2.32 from 2.70 using the feedstuff-meat ratio of feed of the present invention, generally speaking, full period gross weight increases relative to control group
13.9Kg, pig price is calculated with 18 yuan/Kg increases by 250 yuan, and feed cost has only increased 37 yuan, therefore every pig directly increases income about
213 yuan, do not include that medication is reduced, and sick reduction is with good meat quality, and pig price is slightly higher etc. increases income factor, generally speaking, the present invention is used to raise
Material, feeding and economic effect is clearly.
Table 2 is test pig Diet Formula (%)
| Daily ration composition | Test group | Control group |
| Corn | 65 | 65 |
| Dregs of beans | 21 | 21 |
| Premix | 4 | 4 |
| Wheat bran | 0 | 20 |
| Microbiological feed | 20 | 0 |
| It is total | 100 | 100 |
Table 3 is microbiological feed test result
Illustrate: above embodiments are only to illustrate the present invention and not limit the technical scheme described by the invention；Therefore,
Although this specification is referring to above-mentioned each embodiment, the present invention has been described in detail, the common skill of this field
Art personnel should be appreciated that and still can modify to the present invention or equivalent replacement；And all do not depart from spirit of the invention and
The technical solution and its improvement of range, should all cover in scope of the presently claimed invention.
Claims (10)
1. a kind of Soybean Peptide feed making method of high efficiency, low cost, which is characterized in that carry out as steps described below:
(1) bacillus licheniformis, candida utili and lactic acid bacteria are inoculated into fluid nutrient medium respectively and carry out activation culture, is obtained
To the lichen bacillus ferments seed liquor, candida utili fermentation seed liquid and lactobacillus-fermented seed liquor；
(2) configure a phase fermentation medium: in bean dregs add papain, bromelain, brown sugar, potassium dihydrogen phosphate and
Epsom salt is uniformly mixed, obtains a phase fermentation medium；
(3) the lichen bacillus ferments seed liquor and candida utili fermentation seed liquid that inoculation step (1) obtains are in step
(2) in the phase fermentation medium obtained, water content is adjusted, fermentation temperature carries out a phase bacterium enzyme cooperative fermentation, one timing of fermentation
Between after obtain a phase fermenting mixture；
(4) the lactobacillus-fermented seed liquor that inoculation step (1) obtains is in the phase fermenting mixture that step (3) obtains, then plus
Enter acid protease, is uniformly mixed, obtains the second stage of fermenting mixture；The second stage of anaerobic fermentation, anaerobism hair are carried out under air-proof condition
Soybean peptide feed is obtained after ferment.
2. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1, which is characterized in that step
Suddenly each component weight percentage of a phase aerobic fermentation culture medium described in (2) is respectively as follows: bean dregs 97%-99.7%, brown sugar
0.1%-0.7%, potassium dihydrogen phosphate 0.05%-0.4%, epsom salt 0.01%-0.5%, papain and pineapple egg
The total addition level of white enzyme is 0.05%-1.8%.
3. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1 or 2, feature exist
In the enzyme activity of the papain is 200,000 u/g, and the enzyme activity of bromelain is 300,000 u/g.
4. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1, which is characterized in that step
Suddenly the lichen bacillus ferments seed liquor described in (3) and the inoculum concentration of candida utili fermentation seed liquid are phase fermentation
The 0.1%-8% of culture medium weight；The total viable count of the bacillus licheniformis and candida utili is 1.5 × 109-5
×109cfu/mL。
5. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1, which is characterized in that step
Suddenly adjusting water content described in (3) is 35%-75%；The temperature of the fermentation is 20-37 DEG C, time 6-12h.
6. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1, which is characterized in that step
Suddenly lactobacillus-fermented seed liquor described in (4) includes lactobacillus acidophilus fermentation seed liquid, Lactobacillus rhamnosus fermentation seed liquid, does
Lactobacillus paracasei fermentation seed liquid and lactobacillus plantarum fermentation seed liquid；The total viable count of the lactobacillus-fermented seed liquor be 2 ×
109-9×109Cfu/mL, inoculum concentration are the 0.2%-12% of fermenting mixture weight.
7. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1 or 6, feature exist
In, when the lactobacillus-fermented seed liquor is lactobacillus plantarum fermentation seed liquid and lactobacillus acidophilus fermentation seed liquid, two kinds of bacterium
Inoculum concentration ratio be 1:1；The lactobacillus-fermented seed liquor is that Lactobacillus rhamnosus fermentation seed liquid and lactobacillus plantarum are sent out
When ferment seed liquor, the inoculum concentration ratio of two kinds of bacterium is 1:4.
8. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1, which is characterized in that step
Suddenly the additive amount of acid protease described in (4) is the 0.01%-0.5% of a phase fermenting mixture weight；The acidic protein
The enzyme activity of enzyme is 60,000 u/g.
9. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1, which is characterized in that step
Suddenly the water content of second phase fermenting mixture described in (4) is 40%-80%.
10. a kind of Soybean Peptide feed making method of high efficiency, low cost according to claim 1, which is characterized in that
The time of the fermentation of second phase described in step (4) is 3-9 days, and temperature is 20-37 DEG C.
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| CN111631266A (en) * | 2020-06-04 | 2020-09-08 | 龙王致新(北京)科技有限公司 | Preparation method of peptide-containing high-protein soybean milk powder |
| CN113317413A (en) * | 2021-06-04 | 2021-08-31 | 江苏大学 | Processing method and application of hermetia illucens-based aquatic fermented feed |
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| CN102232477A (en) * | 2010-04-28 | 2011-11-09 | 鹤壁市立世蛋白质饲料有限公司 | Biological enzyme protein feed additives and production method thereof |
| CN109511810A (en) * | 2018-12-28 | 2019-03-26 | 浙江康星生物科技有限公司 | A kind of preparation method and applications of the microbiological feed for milking sow |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232477A (en) * | 2010-04-28 | 2011-11-09 | 鹤壁市立世蛋白质饲料有限公司 | Biological enzyme protein feed additives and production method thereof |
| CN109511810A (en) * | 2018-12-28 | 2019-03-26 | 浙江康星生物科技有限公司 | A kind of preparation method and applications of the microbiological feed for milking sow |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111631266A (en) * | 2020-06-04 | 2020-09-08 | 龙王致新(北京)科技有限公司 | Preparation method of peptide-containing high-protein soybean milk powder |
| CN113317413A (en) * | 2021-06-04 | 2021-08-31 | 江苏大学 | Processing method and application of hermetia illucens-based aquatic fermented feed |
| CN113317413B (en) * | 2021-06-04 | 2024-04-09 | 江苏大学 | Processing method and application of fermented feed for aquatic products based on hermetia illucens |
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