CN111887350B - Fermented traditional Chinese medicine feed additive capable of promoting growth and improving resistance and preparation method thereof - Google Patents
Fermented traditional Chinese medicine feed additive capable of promoting growth and improving resistance and preparation method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
Abstract
The invention discloses a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance, which is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight: 8-11 parts of astragalus, 5-8 parts of angelica, 11-13 parts of dangshen, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb. The invention also discloses a preparation method of the additive. The feed additive prepared by the invention is added into livestock and poultry feed, can obviously promote the growth of livestock and poultry, improve daily gain, and improve the immune function and meat quality of livestock and poultry.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a fermented traditional Chinese medicine feed additive capable of promoting growth and improving resistance and a preparation method thereof.
Background
With the large-scale development of livestock and poultry farming, antibiotics are used as feed additives for promoting animal growth and improving animal organism immunity in a large amount. The drug resistance of pathogenic bacteria is enhanced after long-term use, and the harm such as poisoning can be caused after people eat livestock products with antibiotic residues exceeding standards. Therefore, the search for new green, pollution-free feed additives has become a necessary trend.
The fermented traditional Chinese medicine has the advantages of no drug resistance, no drug residue, green and pollution, various functions and the like, and becomes a research hot spot in recent years. The traditional Chinese medicine contains a plurality of active ingredients, has wide influence on the production performance and immunity of animals, and is a good substitute for antibiotics. The probiotics are nontoxic and have unique physiological effects, and can regulate the balance of intestinal flora, provide abundant nutrients such as small peptides, vitamins and the like for the organism, and improve the oxidation resistance and immunity of the organism. The Chinese medicine fermentation organically combines the two, utilizes beneficial bacteria to ferment the Chinese medicine, increases the release amount of active ingredients of the Chinese medicine, has a large amount of active enzymes in fermentation liquor, can regulate immune function and strengthen resistance after being absorbed by organisms, enables the drug effect of the Chinese medicine to be exerted, can decompose toxic ingredients of the Chinese medicine at the same time, and reduces the damage of the medicine to animal organisms.
The problem of feed safety is widely concerned with the rapid development of the feed industry, and the fermented traditional Chinese medicine with complete functions is a research hotspot, and is pollution-free, purely natural and free of antibiotic residues. The probiotics fermented traditional Chinese medicine has important effects on enhancing curative effect, promoting animal production performance, reducing feed cost, enhancing organism immunity and the like, and has wide application prospect.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide the fermented traditional Chinese medicine feed additive for promoting growth and improving resistance and the preparation method thereof, and the raw materials of the additive are Chinese herbal medicine extracts, so that the additive has the advantages of no toxicity, no harm, no drug resistance and the like, and can efficiently release the active ingredients of the drugs, improve the using effect of the drugs, obviously improve the daily gain of livestock and poultry, reduce the feed conversion ratio, reduce the morbidity and play the roles of promoting growth and improving resistance through the optimized preparation process.
The invention is realized by the following technical scheme:
a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
8-11 parts of astragalus, 5-8 parts of angelica, 11-13 parts of dangshen, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb.
Preferably, the fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus, 6 parts of angelica, 12 parts of radix codonopsis, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb.
Preferably, the microbial inoculum adopted by the fermentation is a composite microbial inoculum, the composite microbial inoculum comprises trichoderma, black fungus and lactobacillus, wherein the concentration of the trichoderma is 0.5-1.0x10 9 CFU/mL, concentration of Aspergillus niger is 0.5-1.0X10 9 CFU/mL, trichoderma concentration of 0.5-1.0X10 9 CFU/mL。
The preparation method of the fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) Weighing the traditional Chinese medicine raw materials according to the weight, chopping, uniformly mixing, and putting into a steam explosion tank for steam explosion treatment to obtain a pretreatment mixture;
(2) Crushing and sieving the pretreatment mixture to obtain pretreatment mixture powder;
(3) Uniformly mixing the composite bacterial liquid, the fermentation medium and the pretreatment mixture powder, and performing closed anaerobic fermentation at 34-36 ℃ for 3-5 days to obtain a fermentation product;
(4) Mixing the fermentation product with proper amount of acetic acid and lipase, reacting at 38-45deg.C for 2-4 hr, and drying.
Preferably, in the step (1), the pressure of the steam explosion treatment is 1-1.5MPa, and the time is 3-5min.
Preferably, in the step (2), the particle size of the pretreated mixture powder is 80-100 mesh.
Preferably, in the step (3), the weight ratio of the composite bacterial liquid, the fermentation medium and the pretreated mixture powder is (0.05-0.07): (20-30): (10-15).
Preferably, the preparation method of the fermentation medium comprises the following steps: weighing 2.5-3.5g glucose, 8-12g peptone, 4-6g yeast extract, 1.5-2.5 and 2.5g K 2 HPO 4 、0.15-0.25g MgSO 4 ·H 2 O, adding a proper amount of water for dissolution, adjusting the pH to 6.8-7.2, and then fixing the volume to 1000 mL.
Preferably, in the step (4), the ratio of the fermentation product, acetic acid and lipase is (30-50) g: (1-2) mL: (0.01-0.05 g), wherein the lipase is at least one of Aspergillus niger lipase, mucor lipase, penicillium lipase and yeast lipase, and the enzyme activity is 5000-15000U/g.
Preferably, in the step (4), the drying temperature is 50-60 ℃.
The invention has the advantages that:
(1) The invention adopts the reasonable formula of the traditional Chinese medicine raw materials such as astragalus, angelica, dangshen, bighead atractylodes rhizome, chinese yam, hawthorn, malt, rhizoma atractylodis and the like. Wherein, the astragalus can directly influence the metabolism in cells, induce the cells of the organism to produce related factors, enhance the specific and nonspecific immunity of the organism, exert the immunoregulation function in multiple layers, raise the specific volume or the hemoglobin content of the red blood cells and increase the number of the red blood cells; the codonopsis pilosula has the effects of tonifying healthy qi, strengthening the blood and hematopoietic systems, enhancing immunity and disease resistance, and remarkably enhancing nonspecific immunity, humoral immunity, cellular immunity and interleukin; fructus crataegi and fructus Hordei Germinatus have effects of regulating qi-flowing, resolving food stagnation, invigorating spleen and stomach. The palatability of the feed can be improved, the appetite of animals can be enhanced, the conversion rate of the feed can be improved, and the quality of animal products can be improved; by reasonably combining the traditional Chinese medicine raw materials, the complementary effect of different action mechanisms of each traditional Chinese medicine can be exerted, so that the daily gain can be improved, the feed cost can be reduced, the organism resistance can be improved, the integral regulation function of the organism can be enhanced, and the growth and the resistance of livestock can be effectively promoted.
(2) In the process for preparing the additive, the compound bacteria consisting of trichoderma, black fungus and lactobacillus are adopted for fermentation of the traditional Chinese medicine raw materials, and various enzymes and various secondary metabolites can be generated in the fermentation process by the compound bacteria, so that the structure of the traditional Chinese medicine can be subjected to hydroxylation or dehydrogenation reaction, the medicine property is changed, the medicine effect is improved, a new treatment effect is generated, meanwhile, the traditional Chinese medicine and microorganisms are converted into components which are convenient for absorption and utilization by the organism, the microecological environment of the organism is improved, the health-care effect and the curative effect are improved, and the growth promoting capability and the disease-resistant effect of the additive are further improved.
(3) The invention creatively introduces steam explosion pretreatment and lipase esterification process based on the traditional fermentation process, and forms a combined preparation process of steam explosion pretreatment, composite bacteria fermentation and lipase esterification, wherein the steam explosion pretreatment is carried out firstly, so that the internal structure of the traditional Chinese medicine raw material is more loose, the specific surface area is greatly increased, the number of active groups and reactive sites are increased, the contact opportunity of reactants and catalysts is increased, the composite bacteria fermentation can enable the surface of the traditional Chinese medicine raw material to generate a large number of active hydroxyl groups, and release a large number of active substances such as polysaccharide and the like in the raw material, so that the active substances are easier to be absorbed and utilized by organisms, the antioxidation and growth promotion activities of fermentation products are improved, and then acetic acid and lipase are added to carry out biocatalytic esterification on the fermentation products, so that the activity of enzyme catalytic esterification reaction is improved on the basis of the first two steps, more active hydroxyl groups on the surface of the raw material and the released polysaccharide active substances are involved in esterification reaction, partial ester groups are generated on the surface of the raw material, the polysaccharide is partially esterified with acetic acid, the antioxidation activity and the immunological activity of the polysaccharide active substances are improved, partial hydroxyl groups are converted into ester groups, the gastrointestinal tract is reduced, and the stimulation to the gastrointestinal tract is better improved, and the immunity of the gastrointestinal tract is better improved.
In conclusion, the feed additive prepared by the method is added into livestock and poultry feed through the selection of the formula and the optimization of the preparation process, so that the growth of livestock and poultry can be obviously promoted, the daily gain is improved, and the immune function and the meat quality of the livestock and poultry are improved.
Detailed Description
In the following examples, the Lipase used was Aspergillus niger Lipase (Amano Lipase A), the enzyme activity was 12000U/g, CAS NO:9001-62-1, available from Aldrich.
Example 1
A fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
8-11 parts of astragalus, 5-8 parts of angelica, 11-13 parts of dangshen, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb.
Example 2
A fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
8-11 parts of astragalus, 5-8 parts of angelica, 11-13 parts of dangshen, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb.
The microbial inoculum adopted by the fermentation is a composite microbial inoculum, wherein the composite microbial inoculum comprises trichoderma, black fungus and lactobacillus, and the concentration of the trichoderma is 0.5-1.0x10 9 CFU/mL, concentration of Aspergillus niger is 0.5-1.0X10 9 CFU/mL, trichoderma concentration of 0.5-1.0X10 9 CFU/mL。
Example 3
A fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
9 parts of astragalus, 6 parts of angelica, 12 parts of radix codonopsis, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb.
The microbial inoculum adopted by the fermentation is a composite microbial inoculum, the composite microbial inoculum comprises trichoderma, black fungus and lactobacillus, wherein the concentration of the trichoderma is 0.8x10 9 CFU/mL, concentration of Blackstone mold was 0.6X10 9 CFU/mL, trichoderma concentration of 0.6X10 9 CFU/mL。
Example 4
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) Weighing the following traditional Chinese medicine raw materials in parts by weight: 8 parts of astragalus, 5 parts of angelica, 11 parts of codonopsis pilosula, 8 parts of bighead atractylodes rhizome, 5 parts of Chinese yam, 6 parts of hawthorn, 10 parts of malt, 7 parts of rhizoma atractylodis, 9 parts of liquorice and 8 parts of cockscomb, and after being cut up, uniformly mixing, putting into a steam explosion tank, and performing steam explosion for 5min under the condition of 1MPa to obtain a pretreatment mixture;
(2) Crushing and sieving the pretreatment mixture to obtain pretreatment mixture powder with the granularity of 80 meshes;
(3) The composite bacterial liquid, the fermentation medium and the pretreated mixture powder are mixed according to the weight ratio of 0.05:20:10 are evenly mixed and are subjected to closed anaerobic fermentation for 5 days at 34 ℃ to obtain a fermentation product, wherein the composite bacterial liquid contains 0.5 multiplied by 10 9 Trichoderma reesei in CFU/mL concentration of 0.5X10 9 CFU/mL of Blackmould and concentration of 0.5X10 9 CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 2.5g glucose, 8g peptone, 4g yeast extract, 1.5. 1.5g K 2 HPO 4 、0.15g MgSO 4 ·H 2 O, adding a proper amount of water for dissolution, adjusting the pH value to 6.8, and then fixing the volume to 1000mL to obtain a fermentation medium;
(4) Mixing the fermentation product with acetic acid and lipase according to the proportion of 30g:1mL: mixing 0.01g uniformly, reacting at 38 ℃ for 4 hours, and then drying at 50 ℃ to obtain the catalyst.
Example 5
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) Weighing the following traditional Chinese medicine raw materials in parts by weight: 11 parts of astragalus, 8 parts of angelica, 13 parts of codonopsis pilosula, 9 parts of bighead atractylodes rhizome, 10 parts of Chinese yam, 10 parts of hawthorn, 12 parts of malt, 8 parts of rhizoma atractylodis, 13 parts of liquorice and 10 parts of cockscomb, cutting, uniformly mixing, putting into a steam explosion tank, and performing steam explosion for 3min under the condition of 1.5MPa to obtain a pretreatment mixture;
(2) Crushing and sieving the pretreatment mixture to obtain pretreatment mixture powder with the granularity of 100 meshes;
(3) The composite bacterial liquid, the fermentation medium and the pretreated mixture powder are mixed according to the weight ratio of 0.07:30:15, and performing closed anaerobic fermentation at 36 ℃ for 3 days to obtain a fermentation product, wherein the composite bacterial liquid contains 1.0X10-concentration 9 Trichoderma reesei in CFU/mL concentration of 1.0X10 9 CFU/mL of Blackmould and concentration of 1.0X10 9 CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3.5g glucose, 12g peptone, 6g yeast extract, 2.5. 2.5g K 2 HPO 4 、0.25g MgSO 4 ·H 2 O, adding a proper amount of water for dissolution, adjusting the pH value to 7.2, and then fixing the volume to 1000mL to obtain a fermentation medium;
(4) Mixing the fermentation product with acetic acid and lipase according to the proportion of 50g:2mL: mixing 0.05g uniformly, reacting at 45 ℃ for 2 hours, and then drying at 60 ℃ to obtain the catalyst.
Example 6
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) Weighing the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus, 6 parts of angelica, 12 parts of radix codonopsis, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb, cutting, uniformly mixing, putting into a steam explosion tank, and performing steam explosion for 4min under the condition of 1.4MPa to obtain a pretreatment mixture;
(2) Crushing and sieving the pretreatment mixture to obtain pretreatment mixture powder with the granularity of 90 meshes;
(3) The composite bacterial liquid, the fermentation medium and the pretreated mixture powder are mixed according to the weight ratio of 0.06:25:12 are evenly mixed and are subjected to closed anaerobic fermentation for 4 days at 35 ℃ to obtain a fermentation product, wherein the compound bacteria liquid contains the concentration of 0.8x10 9 Trichoderma reesei in CFU/mL concentration of 0.6X10 9 CFU/mL of Blackmould and concentration of 0.6X10 9 CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3g glucose, 10g peptone, 5g yeast extract, 2g K 2 HPO 4 、0.2g MgSO 4 ·H 2 O, adding a proper amount of water for dissolution, adjusting the pH value to 7, and then fixing the volume to 1000mL to obtain a fermentation medium;
(4) Mixing the fermentation product with acetic acid and lipase according to the proportion of 40g:1.5mL: mixing 0.03g uniformly, reacting at 42 ℃ for 3 hours, and then drying at 55 ℃ to obtain the catalyst.
Example 7
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) Weighing the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus, 6 parts of angelica, 12 parts of radix codonopsis, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb, and after uniform mixing, crushing and sieving to obtain mixed powder with the granularity of 90 meshes;
(2) The composite bacterial liquid, the fermentation medium and the mixed powder are mixed according to the weight ratio of 0.06:25:12 are evenly mixed and are subjected to closed anaerobic fermentation for 4 days at 35 ℃ to obtain a fermentation product, wherein the compound bacteria liquid contains the concentration of 0.8x10 9 Trichoderma reesei in CFU/mL concentration of 0.6X10 9 CFU/mL of Blackmould and concentration of 0.6X10 9 CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3g glucose, 10g peptone, 5g yeast extract, 2g K 2 HPO 4 、0.2g MgSO 4 ·H 2 O, adding a proper amount of water for dissolution, adjusting the pH value to 7, and then fixing the volume to 1000mL to obtain a fermentation medium;
(3) Mixing the fermentation product with acetic acid and lipase according to the proportion of 40g:1.5mL: mixing 0.03g uniformly, reacting at 42 ℃ for 3 hours, and then drying at 55 ℃ to obtain the catalyst.
Example 8
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) Weighing the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus, 6 parts of angelica, 12 parts of radix codonopsis, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb, cutting, uniformly mixing, putting into a steam explosion tank, and performing steam explosion for 4min under the condition of 1.4MPa to obtain a pretreatment mixture;
(2) Crushing and sieving the pretreatment mixture to obtain pretreatment mixture powder with the granularity of 90 meshes;
(3) The composite bacterial liquid, the fermentation medium and the pretreated mixture powder are mixed according to the weight ratio of 0.06:25:12, sealing and anaerobic fermenting at 35deg.C for 4 days, and drying at 55deg.C to obtain the final product, wherein the compound bacteria liquid contains 0.8X10 g 9 Trichoderma reesei in CFU/mL concentration of 0.6X10 9 CFU/mL of Blackmould and concentration of 0.6X10 9 CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3g glucose, 10g peptone, 5g yeast extract, 2g K 2 HPO 4 、0.2g MgSO 4 ·H 2 O, adding a proper amount of water for dissolution, adjusting the pH value to 7, and then fixing the volume to 1000mL to obtain the fermentation medium.
Example 9
A preparation method of a fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) Weighing the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus, 6 parts of angelica, 12 parts of radix codonopsis, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb, and after uniform mixing, crushing and sieving to obtain mixed powder with the granularity of 90 meshes;
(2) The composite bacterial liquid, the fermentation medium and the mixed powder are mixed according to the weight ratio of 0.06:25:12, and performing sealed anaerobic fermentation at 35deg.C for 4 daysThe fermentation product is then dried at 55 ℃ to obtain the composite bacterial liquid with the concentration of 0.8x10 9 Trichoderma reesei in CFU/mL concentration of 0.6X10 9 CFU/mL of Blackmould and concentration of 0.6X10 9 CFU/mL of Trichoderma; the preparation method of the fermentation medium comprises the following steps: weighing 3g glucose, 10g peptone, 5g yeast extract, 2g K 2 HPO 4 、0.2g MgSO 4 ·H 2 O, adding a proper amount of water for dissolution, adjusting the pH value to 7, and then fixing the volume to 1000mL to obtain the fermentation medium.
Test examples
140 fattening pigs are randomly selected in a pig farm, the 140 fattening pigs are randomly divided into 7 groups, each group comprises 20 fattening pigs, the additives prepared in the examples 4-9 are added into basic feed according to 1% of the weight of the feed, after being uniformly mixed, feeding tests are respectively carried out, the basic feed is fed by a blank control group, and the test period is 55 days. The initial weight and the final weight of the fattening pigs before and after each group of test are recorded respectively, and the immune indexes in the fattening pig serum of each group after the test are measured, and the results are shown in tables 1 and 2.
TABLE 1 weight gain results before and after feeding test
TABLE 2 immune index after feeding test
| IgA(g/L) | IgG(g/L) | SOD(u/ml) | MDA(nmol/ml) | |
| Control group | 0.96 | 18.54 | 42 | 4.40 |
| Example 4 | 1.47 | 22.35 | 55 | 3.34 |
| Example 5 | 1.41 | 22.87 | 57 | 3.31 |
| Example 6 | 1.59 | 23.34 | 62 | 3.22 |
| Example 7 | 1.26 | 21.08 | 51 | 3.47 |
| Example 8 | 1.18 | 19.67 | 49 | 3.52 |
| Example 9 | 1.05 | 20.22 | 46 | 4.12 |
As can be seen from the test results of the comparison of the examples 4-9 with the control group, the daily gain of pigs is higher than that of the control group when the additive of the invention is added into the feed; the ratio of IgA, igG and the contrast group is increased, which shows that the immunity of the organism can be improved; the content of SOD is increased, and the content of MDA is reduced, which shows that the protection capability of the antioxidant in the organism can be improved. Therefore, the feed additive can obviously improve the weight of pigs, promote the growth and development of the pigs and improve the immunity of organisms, thereby increasing the economic benefit of breeders.
As can be seen from the test results of comparison of examples 6 to 9, the additive prepared by the steam explosion pretreatment-complex enzyme fermentation-lipase esterification process in example 6 has more remarkable growth promotion and antioxidation effects compared with the additive prepared by the steam explosion pretreatment-complex enzyme fermentation process in example 7, the steam explosion pretreatment-complex enzyme fermentation process in example 8 and the conventional complex enzyme fermentation process in example 9, and has synergistic effects among the working procedures of the additive preparation process, so that the effects of promoting growth and improving immunity of the additive are better exerted.
Claims (6)
1. The fermented traditional Chinese medicine feed additive for promoting growth and improving resistance is characterized by being prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight:
8-11 parts of astragalus membranaceus, 5-8 parts of angelica sinensis, 11-13 parts of codonopsis pilosula, 8-9 parts of bighead atractylodes rhizome, 5-10 parts of Chinese yam, 6-10 parts of hawthorn, 10-12 parts of malt, 7-8 parts of rhizoma atractylodis, 9-13 parts of liquorice and 8-10 parts of cockscomb;
the preparation method of the fermented traditional Chinese medicine feed additive for promoting growth and improving resistance comprises the following steps:
(1) Weighing the traditional Chinese medicine raw materials according to the weight, chopping, uniformly mixing, and putting into a steam explosion tank for steam explosion treatment to obtain a pretreatment mixture;
(2) Crushing and sieving the pretreatment mixture to obtain pretreatment mixture powder;
(3) Uniformly mixing the composite bacterial liquid, the fermentation medium and the pretreatment mixture powder, and performing closed anaerobic fermentation at 34-36 ℃ for 3-5 days to obtain a fermentation product; the compound bacteria liquid contains trichoderma, black mould and lactobacillus, wherein the concentration of the trichoderma is 0.5-1.0x10 9 CFU/mL, concentration of Aspergillus niger is 0.5-1.0X10 9 CFU/mL, trichoderma concentration of 0.5-1.0X10 9 CFU/mL; the weight ratio of the composite bacterial liquid, the fermentation culture medium and the pretreatment mixture powder is (0.05-0.07): (20-30): (10-15);
(4) Mixing the fermentation product with proper amount of acetic acid and lipase, reacting at 38-45deg.C for 2-4 hr, and drying to obtain the final product; the ratio of the fermentation product, acetic acid and lipase is (30-50) g: (1-2) mL: (0.01-0.05 g), wherein the lipase is at least one of Aspergillus niger lipase, mucor lipase, penicillium lipase and yeast lipase, and the enzyme activity is 5000-15000U/g.
2. The fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 1, wherein the additive is prepared by fermenting the following traditional Chinese medicine raw materials in parts by weight: 9 parts of astragalus, 6 parts of angelica, 12 parts of radix codonopsis, 8 parts of bighead atractylodes rhizome, 7 parts of Chinese yam, 8 parts of hawthorn, 11 parts of malt, 7 parts of rhizoma atractylodis, 11 parts of liquorice and 9 parts of cockscomb.
3. The method for preparing the fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 1, wherein in the step (1), the pressure of steam explosion treatment is 1-1.5MPa, and the time is 3-5min.
4. The method for preparing a fermented Chinese medicinal feed additive for promoting growth and improving resistance according to claim 1, wherein in the step (2), the particle size of the pretreated mixture powder is 80-100 meshes.
5. The method for preparing the fermented traditional Chinese medicine feed additive for promoting growth and improving resistance according to claim 1, wherein the preparation method of the fermentation medium is as follows: weighing 2.5-3.5g glucose, 8-12g peptone, 4-6g yeast extract, 1.5-2.5 and 2.5g K 2 HPO 4 、0.15-0.25g MgSO 4 ·H 2 O, adding a proper amount of water for dissolution, adjusting the pH to 6.8-7.2, and then fixing the volume to 1000 mL.
6. The method for preparing a fermented Chinese medicinal feed additive for promoting growth and improving resistance according to claim 1, wherein in the step (4), the drying temperature is 50-60 ℃.
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| CN111388565A (en) * | 2020-05-25 | 2020-07-10 | 开封嘉骏生物科技有限公司 | Traditional Chinese medicine formula for improving hematopoiesis function of female livestock and young livestock and preparation method thereof |
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