CN111513181A

CN111513181A - Production method of enzyme-bacterium combined fermented feed

Info

Publication number: CN111513181A
Application number: CN202010275792.9A
Authority: CN
Inventors: 陈龙; 吴鹏
Original assignee: Ningxia Yizheng Biological Engineering Co ltd
Current assignee: Ningxia Yizheng Biological Engineering Co ltd
Priority date: 2020-04-09
Filing date: 2020-04-09
Publication date: 2020-08-11

Abstract

The invention discloses a production method of enzyme-bacterium combined fermented feed, which comprises a fermentation substrate, composite zymophyte, composite biological enzyme and composite Chinese herbal medicine, wherein the fermentation substrate and the Chinese herbal medicine are respectively and independently crushed, the crushed Chinese herbal medicine is firstly and independently subjected to enzymolysis, the fermentation substrate is mixed with the composite zymophyte and the composite biological enzyme according to the mass fraction, then the mixture is mixed with the Chinese herbal medicine subjected to enzymolysis, and the mixture is subjected to anaerobic bagging fermentation at a certain temperature to obtain an enzyme-bacterium combined fermented feed finished product. The fermented feed can improve the feed intake and weight gain of pigs, regulate and control the internal environment of intestinal tracts, prevent diseases, improve the survival rate, reduce the medicine consumption, has low cost, can effectively reduce the breeding cost of fattening the live pigs, and improves the economic benefit of live pig breeding to the maximum extent.

Description

Production method of enzyme-bacterium combined fermented feed

Technical Field

The invention belongs to the field of feed industry, and particularly relates to a production method of enzyme-bacterium combined fermented feed.

Technical Field

The fermented feed is feed or raw material which is beneficial to animal feeding and utilization and is rich in high-activity probiotics and metabolites thereof, and macromolecular substances and anti-nutritional factors in the feed are decomposed or converted through metabolism and thallus reproduction of microorganisms. The application of the fermented feed in the aspect of animals is more and more common at present, and the fermented feed is used for producing pigs, cattle, sheep and chickens, can reduce the feed cost, adjust the microbial balance in animal herds, improve the sub-health of the herds, produce antibiotic-free high-quality meat and the like. The feed is vital to the growth of animals, and meanwhile, the cost of the feed accounts for more than 70% of the total cost, so that the feed with high quality and low cost is required to improve the breeding benefit and reduce the breeding cost, meanwhile, the breeding quantity of pigs, cattle, sheep and chickens in China is continuously increased, people consider more and more about the nutritional value and safety factors of pork, most of the pig feeds for improving the disease resistance of the pigs in the prior art contain pharmaceutical products, and the pig feeds have great food safety hidden dangers and even harm the health of human bodies after long-term eating of the pork, so that the fermented pig feed which is beneficial to the healthy growth of the pigs and low in cost is urgently needed to be provided.

Disclosure of Invention

Based on the above, there is a need for a method for producing an enzyme-bacterium-combined fermented feed.

A production method of enzyme-bacterium combined fermented feed comprises the following steps:

1. a production method of enzyme-bacterium combined fermented feed is characterized by comprising the following steps:

the enzyme-bacterium combined fermented feed is composed of a fermented substrate, composite zymophytes, composite biological enzymes and composite Chinese herbal medicines, the fermented substrate and the Chinese herbal medicines are respectively and independently crushed, the crushed Chinese herbal medicines are firstly and independently subjected to enzymolysis, the fermented substrate is mixed with the composite zymophytes and the composite biological enzymes according to a certain proportion, then the fermented substrate is mixed with the Chinese herbal medicines subjected to enzymolysis, and the mixture is bagged for anaerobic fermentation at a certain temperature to obtain an enzyme-bacterium combined fermented feed finished product, wherein:

the fermentation substrate variety comprises the following components in percentage by mass: 20-40% of de-seeded pumpkin, 20-40% of rice bran, 5-15% of turmeric leaves, 5-15% of wheat bran, 5-15% of bagasse and 5-15% of bean dregs;

the varieties of the composite zymophyte and the total number of bacterial colonies are as follows: 20 hundred million cfu/g of lactobacillus acidophilus, 10 hundred million cfu/g of enterococcus faecalis, 50 hundred million cfu/g of saccharomyces cerevisiae, 100 hundred million cfu/g of bacillus subtilis, 20 hundred million cfu/g of acetic acid bacteria and 10 hundred million cfu/g of bacillus coagulans;

the compound biological enzyme variety and the enzyme activity are as follows: 2 ten thousand u/g of neutral protease, 1 ten thousand u/g of acid protease, 3 ten thousand u/g of xylanase, 4 ten thousand u/g of glucosidase and 1 ten thousand u/g of low-temperature amylase;

the composite Chinese herbal medicine comprises the following components in percentage by mass: 30% of astragalus root, 20% of folium artemisiae argyi, 10% of scutellaria baicalensis, 20% of honeysuckle and 20% of liquorice;

the method comprises the following specific steps:

(1) firstly, crushing Chinese herbal medicines, mixing the Chinese herbal medicines in proportion to obtain composite Chinese herbal medicine powder, mixing the composite biological enzyme and the composite Chinese herbal medicine powder according to mass percent, adding water accounting for 50 percent of the total mass percent of the mixture to ensure that the water content of the mixture reaches 60 percent, bagging the mixture, and hydrolyzing the mixture for a certain time at constant temperature;

(2) mixing the seed-removed pumpkin and the turmeric leaves according to a certain proportion, and stirring the mixture into paste by a high-speed crusher;

(3) uniformly mixing the product obtained in the step (2) and other fermentation matrixes in a high-speed mixer according to a certain proportion to obtain a fermentation matrix mixture;

(4) adding a certain mass percentage of compound biological enzyme and compound strain into the product in the step (1) and the product in the step (3), and uniformly mixing by using a high-speed mixer to obtain a mixed fermented feed raw material;

(5) putting the mixed fermented feed raw material obtained in the step (4) into a packaging bag containing an inner bag, fastening the opening of the bag by using a rope, stacking and placing in a constant-temperature fermentation chamber for anaerobic fermentation;

(6) and detecting the pH value, and finishing fermentation when the pH value is less than 4.5 to obtain the finished product fermented feed.

Preferably, in the step (1), the addition amount of the compound biological enzyme is 5 per mill of the mass percent of the compound Chinese herbal medicine, the temperature of bagging and constant-temperature enzymolysis is 50 ℃, and the enzymolysis time is 10 hours.

Preferably, in the step (2), the seed-removed pumpkin and the turmeric leaves are mixed according to the mass ratio of 3: 1, mixing, wherein the water content of the seedremoved pumpkin is 82%, and the water content of the turmeric leaves is 75%; the rotating speed of the high-speed crusher is 4000 r/min, and the water content after crushing and mixing is 80%.

Preferably, in the step (3), the fermentation substrates comprise 40% of the product in the step (2), 30% of rice bran, 10% of wheat bran, 10% of bagasse and 10% of bean dregs in percentage by mass; the speed of the high-speed mixer was 1000 rpm.

Preferably, in the step (4), the addition amount of the compound biological enzyme is 1 per mill of the mass percentage of the mixed fermentation substrate.

Preferably, in the step (4), the addition amount of the composite strain is 3 per mill of the mass percentage of the mixed fermentation substrate.

Preferably, in the step (4), the addition amount of the product of the step (1) is 10% of the mass percentage of the mixed fermentation substrate.

Preferably, in the step (4), the water content of the mixed fermented feed raw material is 35-45%.

Preferably, in the step (5), the constant-temperature fermentation temperature is 30-40 ℃, and the stacking height is 2 meters.

The production method of the enzyme-bacterium combined fermented feed comprises the following steps of hydrolyzing the Chinese herbal medicines by using the compound biological enzyme, wherein the hydrolysis purpose is two: one is to release the effective components of the Chinese herbal medicine by biological enzyme, the other is to degrade the effective components containing glycosidic bond such as saponin in the Chinese herbal medicine into aglycon substances by biological enzyme, and hydrolyze the polysaccharide in the Chinese herbal medicine into oligosaccharide, polysaccharide and other substances, and we surprisingly find that the efficacy of the Chinese herbal medicine is improved by 5-10 times by the compound enzymolysis, the main principle is that the saponin substances and the polysaccharide substances can be absorbed and utilized by animals after being hydrolyzed by various enzymes secreted by the animals in the bodies of the animals, but the quantity of various active enzymes secreted by the animals is limited, so the hydrolysis is not thorough, the absorption efficiency of the Chinese herbal medicine in the animals is limited, the effective components of the Chinese herbal medicine are directly hydrolyzed into micromolecule substances by the composite biological enzyme in vitro, and can be directly digested and absorbed by the animals, so the utilization rate of the Chinese herbal medicine in the animals is greatly improved, greatly reduces the animal health care cost.

Detailed Description

The present invention will be described in detail with reference to the following embodiments in order to make the aforementioned objects, features and advantages of the invention more comprehensible. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.

The method for producing the enzyme-bacterium combined fermented feed comprises the following steps:

the Chinese herbal medicine is firstly crushed at a natural pH value with 60 percent of water for enzymolysis, the enzymolysis process is a process of releasing the effective components of the Chinese herbal medicine, the crushing aims to combine the effective components in the Chinese herbal medicine with compound biological enzyme more easily, the contact area between cells is increased, the hydrolysis speed is increased, the main purpose of the water content of 60 percent is that all enzymes are hydrolytic enzyme, the enzymolysis can be better carried out only by the participation of water, but the water content is not too high, and the water is not suitable to be uniformly stirred with a subsequent fermentation substrate and compound bacteria. The adding amount of the compound biological enzyme is 5 per mill of the mass percent of the compound Chinese herbal medicine, aiming at reducing the production cost, a large number of orthogonal experiments prove that 5 per mill of the mass percent of the added Chinese herbal medicine is the optimal adding amount, the temperature of constant-temperature hydrolysis of the bagged Chinese herbal medicine is 50 ℃, and the enzymolysis time is 10 hours. The purpose of thermal insulation hydrolysis is to accelerate the hydrolysis speed, and a large number of experiments prove that the addition amount of the enzyme is 5 per mill, the temperature is 50 ℃, the enzymolysis is carried out for 10 hours, the conversion rate of the saponin converted into the aglycone is more than or equal to 80 percent, the hydrolysis rate of the polysaccharide is more than or equal to 70 percent, and the hydrolysis is relatively thorough.

the pumpkin with seeds removed is planted by a farmer specially for obtaining pumpkin seeds, and when the harvest season comes, the farmer cuts apart the ripe pumpkin and removes the seeds, then the pumpkin meat and the pumpkin pulp are discarded, so that the environment is polluted, the pumpkin meat cannot be fully utilized, and the pumpkin contains a large amount of polysaccharide, much oligosaccharide and nutrient substances of good energy feed and probiotics.

The turmeric leaves are aerial parts of the fresh turmeric, and rhizomes and fruits of the turmeric can be directly used as medicines, so that the turmeric has the effects of clearing damp, clearing heat and detoxifying; researches show that 120 components can be extracted from turmeric and expensive steroid hormone-sapogenin which cannot be synthesized in large quantity by industry; in addition, the rhizome contains 50% of starch, the turmeric leaves also contain saponin compounds, and as the rhizome fruits are only needed for planting the turmeric by farmers, the turmeric leaves and the turmeric stalks are mainly used as agricultural wastes to be directly discarded, so that the environment is polluted, effective substances cannot be utilized, the turmeric leaf can be used as a fermentation feed substrate, the saponin in the turmeric leaves can be hydrolyzed into sapogenin by utilizing the actions of biological enzyme and microbial bacteria, and the starch in the turmeric leaves can be used as a carbon source of the fermentation feed to be utilized by the microorganisms.

the invention makes diversified use of agricultural wastes, the rice bran, the wheat bran and the bagasse all contain a large amount of xylan, and if the materials are directly fed to animals, the digestion and absorption of the animals are not facilitated.

because the types and the contents of enzymes generated by microorganisms in the fermentation process are very low, and the anti-nutritional factors in the matrix cannot be completely degraded, the invention effectively degrades xylan, macromolecular protein, starch and other substances by utilizing the combined action of the biological enzymes and the microorganisms, so that the digestibility of animals is improved.

The invention mainly uses lactic acid bacteria as main materials, lactic acid is anaerobic bacteria, aerobic bacteria bacillus subtilis and bacillus licheniformis are utilized to exhaust oxygen in a packaging bag to provide a better growth environment for the lactic acid bacteria, yeast is facultative anaerobic bacteria and is used for self-reproduction under the aerobic condition, alcohol is produced under the anaerobic condition, the alcohol produced by the yeast can generate acetic acid under the action of acetic acid bacteria, the lactic acid bacteria and bacillus coagulans can produce metabolic product lactic acid, and both the acetic acid and the lactic acid can reduce the pH value of materials, so that a finished product can be stably stored for a long time.

On the other hand, the use amount of acidifier in a farm can be reduced or replaced by the fermented feed, so that the culture cost is reduced; because the Chinese herbal medicine for clearing away heat and toxic materials is added, the drug effect can be greatly improved through enzymolysis, and the addition of the animal protection product can be replaced; because a large amount of metabolites such as enzyme preparations, vitamins, microorganism live bacteria and other substances are generated in the fermentation process, the product of the invention can be utilized by a farm to greatly replace the use amount of the additives.

The moisture content of the mixed raw materials is kept between 35 and 45 percent, and the moisture content is favorable for the rapid increment of the zymophyte.

the purpose of tying the bag opening by using the rope is to leave a certain gap, so that gas generated by aerobic fermentation can be discharged from the packaging bag in a stacking mode, and the bag expansion phenomenon cannot occur after the fermentation is finished.

The pH value is greatly reduced, which indicates that a large amount of acidic substances such as lactic acid, acetic acid and the like are produced in the fermentation process, and the acidic substances can improve the food calling performance of animals and the shelf life of products.

The invention will be further illustrated with reference to the following specific examples.

Example 1

(1) firstly, crushing the Chinese herbal medicines, and mixing 30% of astragalus, 20% of folium artemisiae argyi, 10% of scutellaria, 20% of honeysuckle and 20% of liquorice according to mass percentage to obtain the composite Chinese herbal medicine powder.

Mixing 5 per mill of composite biological enzyme of composite Chinese herbal medicine by mass percent with the composite Chinese herbal medicine powder. The complex enzyme is compatible according to the enzyme activity indexes: 2 ten thousand u/g of neutral protease, 1 ten thousand u/g of acid protease, 3 ten thousand u/g of xylanase, 4 ten thousand u/g of glucosidase and 1 ten thousand u/g of low-temperature amylase.

And adding 50% of water by mass to ensure that the water content of the mixture reaches 60%, bagging, performing constant-temperature enzymolysis at 50 ℃ for 10 hours.

(2) Removing seeds of pumpkin with the water content of 82 percent and turmeric leaves with the water content of 75 percent according to the mass ratio of 3: 1, mixing, and stirring into paste by using a high-speed crusher at the rotating speed of 4000 revolutions per minute; the water content after mixing was 80%.

(3) Mixing the product obtained in the step (2) with other fermentation matrixes according to the mass percentage of 30% of rice bran, 10% of wheat bran, 10% of bagasse and 10% of bean dregs; the speed of the high-speed mixer was 1000 rpm. Forming a fermentation substrate mixture;

(4) and (3) uniformly mixing the product in the step (1) and the product in the step (3) with a compound biological enzyme and a compound strain by a high-speed mixer at 1000 rpm according to a certain mass percentage to obtain a mixed fermented feed raw material with the water content of 35%, wherein the addition amount of the compound biological enzyme is 1 per mill of the mass percentage of the mixed fermented substrate, the addition amount of the compound strain is 3 per mill of the mass percentage of the mixed fermented substrate, and the addition amount of the product in the step (1) is 10 percent of the mass percentage of the mixed fermented substrate.

(5) And (4) putting the mixed fermented feed raw materials obtained in the step (4) into a packaging bag containing an inner bag, fastening the opening of the bag by using a rope, stacking for 2 m, and placing in a fermentation chamber at the temperature of 40 ℃ for anaerobic fermentation.

Example 2

(2) Removing seeds of pumpkin with the water content of 82 percent and turmeric leaves with the water content of 75 percent according to the mass ratio of 8: 1, mixing, and stirring into paste by using a high-speed crusher at the rotating speed of 4000 revolutions per minute; the water content after mixing was 80%.

(3) Mixing 45% of the product obtained in the step (2) with 40% of rice bran, 5% of wheat bran, 5% of bagasse and 5% of beans according to mass percentage of other fermentation matrixes; the speed of the high-speed mixer was 1000 rpm. Forming a fermentation substrate mixture;

(4) and (3) adding a certain mass percent of compound biological enzyme and compound strain into the product obtained in the step (1) and the product obtained in the step (3), and uniformly mixing the mixture by using a high-speed mixer at 1000 r/min to obtain a mixed fermented feed raw material with the water content of 40%, wherein the addition amount of the compound biological enzyme is 1 per mill of the mass percent of the mixed fermented substrate, the addition amount of the compound strain is 3 per mill of the mass percent of the mixed fermented substrate, and the addition amount of the product obtained in the step (1) is 10 percent of the mass percent of the mixed fermented substrate.

(5) And (4) putting the mixed fermented feed raw materials obtained in the step (4) into a packaging bag containing an inner bag, fastening the opening of the bag by using a rope, stacking for 2 m, and placing in a fermentation chamber at the temperature of 35 ℃ for anaerobic fermentation.

Example 3

(2) Removing seeds of pumpkin with the water content of 82 percent and turmeric leaves with the water content of 75 percent according to the mass ratio of 2: 1, mixing, and stirring into paste by using a high-speed crusher at the rotating speed of 4000 revolutions per minute; the water content after mixing was 80%.

(3) Mixing the product obtained in the step (2) with 30% of other fermentation matrixes by mass percent, wherein the mixture of the product is composed of 30% of rice bran by mass percent, 15% of wheat bran by mass percent, 15% of bagasse by mass percent and 10% of bean dregs by mass percent; the speed of the high-speed mixer was 1000 rpm. Forming a fermentation substrate mixture;

(4) and (3) adding a certain mass percent of compound biological enzyme and compound strain into the product obtained in the step (1) and the product obtained in the step (3), and uniformly mixing the mixture by using a high-speed mixer at 1000 r/min to obtain a mixed fermented feed raw material with the water content of 45%, wherein the addition amount of the compound biological enzyme is 1 per mill of the mass percent of the mixed fermented substrate, the addition amount of the compound strain is 3 per mill of the mass percent of the mixed fermented substrate, and the addition amount of the product obtained in the step (1) is 10 percent of the mass percent of the mixed fermented substrate.

(5) And (4) putting the mixed fermented feed raw materials obtained in the step (4) into a packaging bag containing an inner bag, fastening the opening of the bag by using a rope, stacking for 2 m, and placing in a fermentation chamber at the temperature of 30 ℃ for anaerobic fermentation.

Example 4

(3) Mixing 45% of the product obtained in the step (2) with 20% of rice bran, 10% of wheat bran, 10% of bagasse and 15% of bean dregs in other fermentation matrixes in percentage by mass; the speed of the high-speed mixer was 1000 rpm. Forming a fermentation substrate mixture;

Comparative example 1

A production method of compound bacterium fermented feed comprises the following steps:

(1) firstly, crushing Chinese herbal medicines, mixing 30% of astragalus, 20% of folium artemisiae argyi, 10% of scutellaria baicalensis, 20% of honeysuckle and 20% of liquorice according to mass percentage to form composite Chinese herbal medicine powder, and then adding 50% of water according to the total mass percentage to ensure that the water content of the composite Chinese herbal medicine powder reaches 60%.

(4) and (3) adding a certain mass percent of composite strains into the product obtained in the step (1) and the product obtained in the step (3), and uniformly mixing the mixture by using a high-speed mixer at 1000 r/min to obtain a mixed fermented feed raw material with the water content of 35%, wherein the adding amount of the composite strains is 3 per mill of the mass percent of the mixed fermented substrate, and the adding amount of the product obtained in the step (1) is 10 percent of the mass percent of the mixed fermented substrate.

After the compound Chinese herbal medicine is subjected to enzymolysis, chemical components are greatly changed, saponin is hydrolyzed into sapogenin under the conversion of the compound enzyme preparation, the digestion and absorption of animals are facilitated, and detection results are shown in table 1.

TABLE 1 conversion of sapogenin after enzymolysis of composite Chinese herbal medicine

Group of	The content of saponin%	Sapogenin content%	Percentage of hydrolysis%
				Comparative example 1 (non-enzymatic)	1.25	0	0
Example 1	0.23	1.02	81.6
				Example 2	0.20	1.05	84.0
Example 3	0.16	1.09	87.2
				Example 4	0.24	1.01	80.8

As can be seen from Table 1, the conversion rate of the saponins is more than or equal to 80% after the Chinese herbal medicines are hydrolyzed by the enzyme, which indicates that the conversion rate of the saponins is greatly improved after the Chinese herbal medicines are hydrolyzed by the enzyme, and the digestion and absorption of animals to the saponins are facilitated.

Because the fermentation substrates rice bran, wheat bran and bagasse contain a large amount of xylan, the xylan is difficult to degrade by directly adding the composite bacteria for fermentation, and because the degraded xylan mainly depends on the degradation of the biological enzyme, but the amount of the biological enzyme generated in the process of only adding the composite bacteria for fermentation is very limited, and the xylan in the substrates cannot be hydrolyzed completely, the composite biological enzyme and the composite bacteria are added for combined action, so that the degradation of the anti-nutritional factor xylan is more complete, and the detection results are shown in table 2.

TABLE 2 xylan content after fermentation

Group of	Content of xylan%	The degradation rate%
			Comparative example 1 (before fermentation)	3.07	0
Comparative example 1 (after fermentation)	2.89	5.8
			Example 1	0.78	74.5
Example 2	0.69	77.5
			Example 3	0.81	73.6
Example 4	0.72	76.5

As can be seen from Table 2, the xylan content changes greatly before and after the enzyme-bacterium combined fermentation and the separate bacterium-adding fermentation, the xylan is hardly degraded before and after the fermentation by the separate bacterium-adding fermentation, the xylan degradation efficiency is higher by the enzyme-bacterium combined fermentation, and the xylan content degradation rate is more than or equal to 70 percent in the examples.

The amount of lactobacillus in the fermented feed obtained in each example was measured according to the method in GB4789.35-2010 national food safety standard test for microbiological examination of lactic acid bacteria. The results are shown in Table 3.

TABLE 3 fermented feed test results

Group of	Number of lactic acid bacteria (hundred million/g)	Lactic acid content (%)	pH of the final product
				Example 1	3.9	2.27	4.25
Example 2	5.5	3.15	4.11
				Example 3	5.2	3.29	4.32
Example 4	4.7	3.03	4.18
				Comparative example 1	1.3	1.13	4.50

As can be seen from the detection results in Table 3, the fermented feed produced by the method of the invention has the viable count of lactobacillus of more than 3 hundred million/g, the content of lactic acid of more than 2 percent, and the pH value of the finished fermented feed is below 4.5, thereby being very beneficial to the preservation of the fermented feed and avoiding the propagation of mixed bacteria.

The main reason why the ratio of lactic acid bacteria to lactic acid is higher in the method of the present invention is that in the early stage of fermentation, the added biological enzyme hydrolyzes macromolecular xylan into xylo-oligosaccharide, which is a nutrient substance of various beneficial bacteria (such as lactic acid bacteria, yeast, etc.), so compared with the comparative example 1 without adding the compound biological enzyme, the propagation speed of the bacteria is fast, the aging speed is slow, and the acid production capability is strong, so that the corresponding lactic acid content is higher than that of the comparative example 1 by more than 50%, and a significant effect is achieved.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

the method comprises the following specific steps:

2. The production method of the enzyme-bacterium combined fermented feed as claimed in claim 1, wherein in the step (1), the addition amount of the compound bio-enzyme is 5 per mill of the mass percent of the compound Chinese herbal medicine, the temperature of bagging and constant temperature enzymolysis is 50 ℃, and the enzymolysis time is 10 hours.

3. The production method of the enzyme-bacterium combined fermented feed according to claim 1, wherein in the step (2), the seed-removed pumpkin and the turmeric leaves are mixed according to a mass ratio of 3: 1, mixing, wherein the water content of the seedremoved pumpkin is 82%, and the water content of the turmeric leaves is 75%; the rotating speed of the high-speed crusher is 4000 r/min, and the water content after crushing and mixing is 80%.

4. The production method of the enzyme-bacterium combined fermented feed as claimed in claim 1, wherein in the step (3), the mass percentages of the fermentation substrates are 40% of the product obtained in the step (2), 30% of rice bran, 10% of wheat bran, 10% of bagasse and 10% of bean dregs; the speed of the high-speed mixer was 1000 rpm.

5. The method for producing the enzyme-bacterium combined fermented feed according to claim 1, wherein in the step (4), the addition amount of the compound bio-enzyme is 1% per mill of the mass percentage of the mixed fermentation substrate.

6. The method for producing the enzyme-bacterium combined fermented feed according to claim 1, wherein in the step (4), the addition amount of the composite strain is 3% per mill of the mass percentage of the mixed fermentation substrate.

7. The method for producing enzyme-microorganism-combined fermented feed according to claim 1, wherein in the step (4), the amount of the product obtained in the step (1) is 10% by mass of the mixed fermentation substrate.

8. The method for producing enzyme-microorganism-combined fermented feed according to claim 1, wherein the water content of the raw material for mixed fermented feed in the step (4) is 35% to 45%.

9. The method for producing enzyme-bacterium combined fermented feed according to claim 1, wherein in the step (5), the constant-temperature fermentation temperature is 30 ℃ to 40 ℃ and the stacking height is 2 m.