CN116509982A - Fermented traditional Chinese medicine preparation for treating porcine diarrhea and application thereof - Google Patents
Fermented traditional Chinese medicine preparation for treating porcine diarrhea and application thereof Download PDFInfo
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Classifications
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- A61K36/344—Codonopsis
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- A—HUMAN NECESSITIES
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- A61K31/19—Carboxylic acids, e.g. valproic acid
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Abstract
The invention discloses a fermented traditional Chinese medicine preparation for treating porcine diarrhea and application thereof, and belongs to the technical field of livestock and veterinary medicine. The fermented traditional Chinese medicine preparation is obtained by fermenting dangshen, bighead atractylodes rhizome, poria cocos, dried ginger, medicated leaven, pericarpium granati and compound electrolyte by using probiotics mother liquor; the probiotic mother liquor contains bacillus subtilis, saccharomyces cerevisiae, lactobacillus and enterococcus faecalis. The preparation method of the fermented traditional Chinese medicine preparation comprises the following steps: (1) Mixing the Chinese herbal medicines, crushing to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, and curing the mixed solution to obtain a Chinese herbal medicine mixture; (2) And adding the probiotic mother liquor and the compound electrolyte into the traditional Chinese medicine mixture, and fermenting to obtain the traditional Chinese medicine fermentation liquor. The Chinese medicinal preparation can resist diarrhea and electrolyte disturbance caused by intestinal bacteria and virus infection, has remarkable control effect on diarrhea caused by intestinal coronavirus infection, is convenient to use, is beneficial to industrial production, and has high economic benefit.
Description
Technical Field
The invention belongs to the technical field of livestock and veterinary medicine, and particularly relates to a fermented traditional Chinese medicine preparation for treating porcine diarrhea and application thereof.
Background
Porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus, porcine delta coronavirus, porcine acute diarrhea syndrome coronavirus, and porcine rotavirus are common enteroviruses. The pathogenic infection causes gastrointestinal inflammation, septicemia, diarrhea, intestinal hemorrhage, etc. Salmonella enteritidis and escherichia coli are common intestinal tract conditional pathogenic bacteria. Often causes secondary infection, can cause huge influence in the breeding industry, and antibiotics can not control the diseases, so that a need exists for searching new medicines.
The Chinese traditional medicine has the advantages of abundant resources, various types, large yield, long drug effect time, administration according to syndrome differentiation, broad-spectrum pathogen resistance and the like; traditional processing of traditional Chinese medicine comprises means of decocting, boiling, stir-frying, steaming, soaking and the like, the processing depth is not enough, and effective substances are difficult to fully absorb and utilize. Therefore, in order to improve the utilization rate and the extraction rate, enhance the drug effect, the fermentation of probiotics is important to the exertion of the traditional Chinese medicine components; meanwhile, the probiotics and the traditional Chinese medicine cooperatively play pharmacological roles, so that not only can the traditional Chinese medicine resources be saved, but also the medicine effect can be improved.
Probiotics (Probiotics) are a class of active microorganisms that are beneficial to the host to maintain the balance of intestinal flora. Probiotics are classified into three major categories, (1) lactobacillus species, (2) bifidobacterium species. (3) Gram positive cocci such as Streptococcus faecalis, lactococcus, streptococcus intermedius, etc. In addition, some yeasts and enzymes may also fall into the category of probiotics. The effects of probiotics include: 1. helping digestion and absorption of nutrients, and generating digestive enzymes in the gastrointestinal tract of the probiotic bacterial strain to help absorb food nutrients. Probiotics competitively inhibit harmful microorganisms from absorbing nutrients and entering the blood circulation system. 2. The probiotics can produce various vitamins, short-chain fatty acids, antioxidants, amino acids and the like. 3. The immunity is improved against bacterial and viral infections, and the probiotics produce chemicals that kill harmful bacteria and compete with the harmful bacteria for space and resources to inhibit their growth. Inhibit the production of toxins by harmful bacteria. And (3) removing toxins produced by harmful bacteria. Preventing and treating intestinal syndrome, ulcer, etc.
The probiotic fermented traditional Chinese medicine has the effects of improving the content of active ingredients, expanding indications, enhancing the efficacy, regulating intestinal flora and reducing the toxicity of the traditional Chinese medicine; the modern fermentation technology of traditional Chinese medicine is developed by combining traditional fermentation technology with microbiology, and probiotics are used for fermenting traditional Chinese medicine, so that multi-stage combined fermentation of various bacteria is utilized, reciprocal and reciprocal among strains is realized, and the production efficiency and the content of active ingredients can be improved.
Disclosure of Invention
The invention aims at providing a fermented traditional Chinese medicine preparation for treating porcine diarrhea, which is obtained by fermenting Chinese herbal medicines and compound electrolytes by using probiotics mother liquor; the Chinese herbal medicines comprise radix Codonopsis, atractylodis rhizoma, poria, zingiberis rhizoma, massa Medicata Fermentata, and pericarpium Granati; the probiotic mother liquor contains bacillus subtilis, saccharomyces cerevisiae, lactobacillus and enterococcus faecalis.
Preferably, the compound electrolyte consists of sodium chloride, sodium phosphate and potassium citrate, wherein each liter of water contains 3.5g of sodium chloride, 2.5g of sodium phosphate and 1.5g of potassium citrate.
More preferably, the Chinese herbal medicine comprises the following components in parts by weight: 2-3 parts of codonopsis pilosula, 2-3 parts of bighead atractylodes rhizome, 1-2 parts of poria cocos, 1-2 parts of dried ginger, 1-3 parts of pericarpium granati and 1-3 parts of medicated leaven.
More preferably, the 4 bacteria in the probiotic mother liquor are 2-4 parts of an activating solution of enterococcus faecium, 2-4 parts of an activating solution of lactobacillus, 2-4 parts of an activating solution of saccharomyces cerevisiae and 0.5-1 part of an activating solution of bacillus subtilis in parts by weight.
The second object of the present invention is to provide a method for preparing the fermented traditional Chinese medicine preparation, which comprises the following steps:
(1) Mixing the Chinese herbal medicines, crushing to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, and curing the mixed solution to obtain a Chinese herbal medicine mixture;
(2) And adding the probiotic mother liquor and the compound electrolyte into the traditional Chinese medicine mixture, and fermenting to obtain traditional Chinese medicine fermentation liquor.
Preferably, in said step (1), said mixture a is subjected to a reaction according to 5:1 weight of any one of molasses, corn flour or milk is added and then mixed with water.
More preferably, the curing treatment in the step (1) comprises the following steps: the mixture was steamed and heated to 80℃for 20min.
More preferably, in the step (2), probiotics and compound electrolyte are added into the traditional Chinese medicine mixture at the temperature of 35-40 ℃, and the weight ratio of the probiotics mother liquor, the electrolyte and the traditional Chinese medicine mixture is 0.5-2:0.1:10.
the invention also aims to provide an application of the fermented traditional Chinese medicine preparation in preparing a product for treating porcine diarrhea.
Compared with the prior art, the invention has the following beneficial effects:
the traditional Chinese medicine preparation provided by the invention can effectively improve the survival rate of piglets, reduce the morbidity, effectively improve the immune function of the piglets after long-term feeding, ensure the intestinal digestion level and the like, can be used as a high-quality medicine or feed additive for breeding live pigs, especially for breeding piglets, and has a good treatment effect on enterocoronavirus infectious diarrhea.
Detailed Description
Example 1
The preparation method of the four activated bacteria comprises the following steps:
(1) Will 10 8 Inoculating enterococcus faecium/uL into a lactobacillus culture medium according to an inoculum size of 0.5%, and culturing at 25 ℃ for 2 hours to obtain mother liquor of the enterococcus faecium;
(2) Will 10 8 Inoculating uL bacillus subtilis into a bacillus subtilis culture medium according to an inoculum size of 0.2%, and culturing for 2 hours at 35 ℃ to obtain a bacillus subtilis mother liquor;
(3) Will 10 8 Inoculating uL Saccharomyces cerevisiae into a fungus culture medium according to an inoculum size of 0.5%, and culturing at 28 ℃ for 2 hours to obtain mother liquor of the Saccharomyces cerevisiae;
(4) Will 10 8 Inoculating uL lactobacillus into lactobacillus agar culture medium according to 0.5% inoculum size, and culturing at 28deg.C for 2 hr to obtain lactobacillus mother liquor;
the compound electrolyte formula comprises 3.5g of sodium chloride, 2.5g of sodium phosphate and 1.5g of potassium citrate in each liter of water.
Example 2
The embodiment provides a fermented traditional Chinese medicine preparation for treating porcine diarrhea, which specifically comprises the following steps:
step (1): the traditional Chinese medicines are mixed and crushed, and the weight ratio is 10:1 adding molasses to obtain a mixture A, adding water according to the volume ratio of the mixture A to the water of 10:3, mixing to obtain a mixed solution, introducing steam into the mixed solution, heating to 80 ℃, continuing curing treatment for 20min, and cooling to 35-40 ℃ to obtain a cured traditional Chinese medicine mixture;
step (2): adding the probiotic mother liquor and the compound electrolyte into the cured traditional Chinese medicine mixture, wherein the probiotic mother liquor is prepared by the following steps: an electrolyte: the weight ratio of the traditional Chinese medicine mixture is 0.5:0.1:10, keeping the temperature of 37 ℃ for shake fermentation for 48 hours to obtain the fermented traditional Chinese medicine;
the Chinese herbal medicine comprises the following components in parts by weight: 2 parts of codonopsis pilosula, 3 parts of bighead atractylodes rhizome, 1 part of poria cocos, 1 part of dried ginger, 3 parts of pericarpium granati and 3 parts of medicated leaven;
the probiotic mother liquor is prepared by mixing 4 parts of the enterococcus faecalis mother liquor, 4 parts of the lactobacillus mother liquor, 2 parts of the brewing yeast liquor and 1 part of the bacillus subtilis mother liquor prepared in the embodiment 1 in parts by weight.
Example 3
The embodiment provides a fermented traditional Chinese medicine preparation for treating porcine diarrhea, which specifically comprises the following steps:
step (1): the Chinese herbal medicines are mixed and crushed, and the weight ratio is 10:1 adding milk to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, introducing steam into the mixed solution, heating to 80 ℃, curing for 20min, and standing at 35-40 ℃ to obtain a cured traditional Chinese medicine mixture;
step (2): adding the probiotic mother liquor and the compound electrolyte into the traditional Chinese medicine mixture, wherein the probiotic mother liquor is as follows: an electrolyte: the weight ratio of the traditional Chinese medicine mixture is 1:0.1:10, fermenting the raw materials to obtain a raw material fermentation liquid;
in the step (1), when the mixture A is mixed with water, the volume ratio of the mixture A to the water is 10:4;
in the step (2), the temperature of raw material fermentation is 37 ℃, and the shaking table shake fermentation time is 36 hours, so as to obtain fermented traditional Chinese medicine liquid;
the Chinese herbal medicine comprises the following components in parts by weight: 3 parts of codonopsis pilosula, 2 parts of bighead atractylodes rhizome, 2 parts of poria cocos, 2 parts of dried ginger, 1 part of pericarpium granati and 1 part of medicated leaven;
the probiotic mother liquor is prepared by mixing 3 parts of the enterococcus faecalis mother liquor, 3 parts of the lactobacillus mother liquor, 2 parts of the brewing yeast liquor and 1 part of the bacillus subtilis mother liquor prepared in the embodiment 1 in parts by weight.
Example 4
The piglet in a certain scale farm has severe diarrhea, and the pathogen is confirmed to be the pig delta coronavirus infection according to the clinical symptoms, pathological changes and laboratory diagnosis of the sick piglet. The 80 piglets with confirmed diagnosis were selected and randomly divided into 4 groups, 20 for each group, which are blank group, test 1 group (example 1), test 2 group (example 2). The experimental period was 12 days, and each group was treated as follows:
blank group: feeding milk;
test 1 group: feeding milk +4g/head/day the fermented Chinese medicinal preparation prepared in example 2, 2 times daily for 7 consecutive days
Test 2 group: the fermented Chinese herbal preparation prepared in example 3 was fed with milk +4 g/head/day, 2 times daily for 7 consecutive days.
TABLE 1 Effect of fermented Chinese medicinal preparation on piglet delta coronavirus infection
TABLE 2 Effect of fermented Chinese medicinal preparation on piglet growth Properties
| Group of | Initial body weight (kg) | Experimental powder body weight (kg) | Growth rate |
| Blank group | 2.21±0.05 | 2.05±0.25 | -7％ |
| Test 1 group | 2.16±0.08 | 2.41±0.33 | 12％ |
| Test 2 groups | 2.01±0.14 | 2.38±0.67 | 18％ |
Test results show that compared with the blank group, the cure rate of the test 1 group and the test 2 group is improved, the death rate is reduced, the cure rate is between 70% and 75%, and the death rate is between 25% and 30%. The weight of the piglets in the blank group is reduced by 7% before and after the experiment, and the weight of the piglets in the experiment group is respectively increased by 12% and 18%. The medicine has the anti-diarrhea effect, and shortens the course of disease.
The embodiment of the invention provides a fermented traditional Chinese medicine preparation for resisting infectious diarrhea, probiotics in raw materials take Chinese herbal medicines as fermentation substrates, and various vitamins, short-chain fatty acids, antioxidants, amino acids and aromatic substances are generated in the fermentation process, so that the bitter and astringent smell of the traditional Chinese medicine can be reduced and eliminated, and the palatability is improved. Under the fermentation action of probiotics, the cell wall of the traditional Chinese medicine plant can be promoted to be ruptured to release active ingredients. The fermented compound traditional Chinese medicine composition can antagonize pig intestinal infectious pathogen and treat pig diarrhea.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (9)
1. The fermented traditional Chinese medicine preparation for treating the diarrhea of pigs is characterized by being obtained by fermenting Chinese herbal medicines and compound electrolytes by using probiotics mother liquor; the Chinese herbal medicines comprise radix Codonopsis, atractylodis rhizoma, poria, zingiberis rhizoma, massa Medicata Fermentata, and pericarpium Granati; the probiotic mother liquor contains bacillus subtilis, saccharomyces cerevisiae, lactobacillus and enterococcus faecalis.
2. The fermented traditional Chinese medicine preparation according to claim 1, wherein the compound electrolyte consists of sodium chloride, sodium phosphate and potassium citrate, and the prescription components comprise 3.5g of sodium chloride, 2.5g of sodium phosphate and 1.5g of potassium citrate.
3. The fermented traditional Chinese medicine preparation according to claim 2, wherein the specific parts of the components in the Chinese herbal medicine are as follows: 2-3 parts of codonopsis pilosula, 2-3 parts of bighead atractylodes rhizome, 1-2 parts of poria cocos, 1-2 parts of dried ginger, 1-3 parts of pericarpium granati and 1-3 parts of medicated leaven.
4. The fermented traditional Chinese medicine preparation according to claim 3, wherein the 4 bacteria in the probiotic mother liquor are 2-4 parts by weight of an activating solution of enterococcus faecium, 2-4 parts by weight of an activating solution of lactic acid bacteria, 2-4 parts by weight of an activating solution of saccharomyces cerevisiae and 0.5-1 part by weight of an activating solution of bacillus subtilis.
5. A method of preparing the fermented traditional Chinese medicine formulation of any one of claims 1-4, characterized in that the method comprises the steps of:
(1) Mixing the Chinese herbal medicines, crushing to obtain a mixture A, mixing the mixture A with water to obtain a mixed solution, and curing the mixed solution to obtain a Chinese herbal medicine mixture;
(2) And adding the probiotic mother liquor and the compound electrolyte into the traditional Chinese medicine mixture, and fermenting to obtain traditional Chinese medicine fermentation liquor.
6. The method of claim 5, wherein in step (1), the mixture a is mixed according to 5:1 weight of any one of molasses, corn flour or milk is added and then mixed with water.
7. The method according to claim 6, wherein the curing treatment in the step (1) is performed by: the mixture was steamed and heated to 80℃for 20min.
8. The method according to claim 6 or 7, wherein in the step (2), probiotics and compound electrolyte are added into the traditional Chinese medicine mixture at the temperature of 35-40 ℃, and the weight ratio of the probiotics mother liquor, the electrolyte and the traditional Chinese medicine mixture is 0.5-2:0.1:10.
9. use of the fermented traditional Chinese medicine preparation according to any one of claims 1-4 for preparing a product for treating porcine diarrhea.
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