CN108077125A - Improve the cultural method of Tilapia mossambica oxidation resistance - Google Patents
Improve the cultural method of Tilapia mossambica oxidation resistance Download PDFInfo
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- CN108077125A CN108077125A CN201711436924.6A CN201711436924A CN108077125A CN 108077125 A CN108077125 A CN 108077125A CN 201711436924 A CN201711436924 A CN 201711436924A CN 108077125 A CN108077125 A CN 108077125A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to the cultural method of technical field of aquaculture, more particularly to raising Tilapia mossambica oxidation resistance, including following preparation process：Conventional Tilapia mossambica special feed is taken to be crushed to 200 300 mesh, then is uniformly mixed with traditional Chinese medicine powder, is granulated, conventionally carries out feeding Tilapia mossambica；The amount of the traditional Chinese medicine powder is the 0.04 0.12% of conventional Tilapia mossambica special feed weight；The traditional Chinese medicine powder is made of the raw material of following parts by weight：10 20 parts of Ramulus Taxilli, 15 30 parts of eclipta, 7 17 parts of Common Achyranthes Herb, 28 parts of rough melic herb.The present invention is improved in the cultural method of Tilapia mossambica oxidation resistance, and Tilapia mossambica special feed with traditional Chinese medicine powder is mixed and feeds Tilapia mossambica, can significantly improve meat quality of java tilapia oxidation resistance, promotes the economic benefit of Tilapia mossambica.
Description
Technical field
The invention belongs to the cultural methods of technical field of aquaculture, more particularly to raising Tilapia mossambica oxidation resistance.
Background technology
Tilapia mossambica is commonly called as：African crucian, non-crucian carp, Vietnam fish, Nan Yang crucian carp etc..Original refers to using Mozambique as type locality
Mouth incubates non-crucian carp category fish species：Non- crucian carp (scientific name is incubated in Mozambique mouthful：Oreochromis mossambicus), it is now Family Cichlidae
Non- crucian carp category and mouth incubate non-crucian carp category etc. and belong to being commonly called as jointly for several fish；Tilapia is referred to as in English.It is world's aquatic products now
One of the cultured freshwater fish of the emphasis scientific research culture of industry, and be known as the main source of following animal protein.It is usually raw
It is living also to be moved in the salt water of different salt contents in fresh water, lake can also be survived in, river, in the shallow water in pond.It has
Very strong adaptability can also breed in the narrow and small waters of area or even can be grown in rice terrace, and less to dissolved oxygen
Water have extremely strong adaptability.Most Tilapia mossambicas are omnivorousness, often eat aquatic plant and mince.
The meat flavour of Tilapia mossambica is delicious, fine and tender taste, either braises in soy sauce or cooks clearly, taste is all good.Bolti is through surveying
It is fixed, containing 20.5 grams of protein, 6.93 grams of fat, 148 kilocalories of heat, 70 milligrams of calcium, 50 milligrams of sodium, 37 milli of phosphorus in every 100 grams of meat
Gram, 1 milligram of iron, 0.1 milligram of vitamin B1,0.12 milligram of vitamin B2.Tilapia mossambica, which has become international, mainly cultivates fish
Class.
For how to improve the cultural method of Tilapia mossambica oxidation resistance, by retrieval, correlative study is found no.
The content of the invention
The technical problems to be solved by the invention are to provide the cultural method for improving Tilapia mossambica oxidation resistance.
To achieve the above object, the present invention adopts the following technical scheme that：
The present invention provides the cultural method for improving Tilapia mossambica oxidation resistance, including following preparation process：Take conventional Rofe
Fish special feed is crushed to 200-300 mesh, then is uniformly mixed with traditional Chinese medicine powder, is granulated, conventionally carries out feeding Rofe
Fish.
The amount of traditional Chinese medicine powder of the present invention is the 0.04-0.12% of conventional Tilapia mossambica special feed weight.
Traditional Chinese medicine powder of the present invention is made of the raw material of following parts by weight：10-20 parts of Ramulus Taxilli, eclipta 15-30
Part, 7-17 parts of Common Achyranthes Herb, 2-8 parts of rough melic herb.
Preferably, the amount of traditional Chinese medicine powder of the present invention is the 0.08% of conventional Tilapia mossambica special feed weight.
Preferably, traditional Chinese medicine powder of the present invention is made of the raw material of following parts by weight：15 parts of Ramulus Taxilli, eclipta 22
Part, 12 parts of Common Achyranthes Herb, 5 parts of rough melic herb.
Preferably, Chinese medicine powder, preparation method thereof of the present invention is：Whole raw materials are weighed by weight, are uniformly mixed, powder
150-250 mesh is broken to, adds in the ethanol solution of whole 15-30 times of raw material weights, refluxing extraction three times, extracts 40- every time
60min merges extracting solution, concentrates, spray drying.
Preferably, in Chinese medicine powder, preparation method thereof of the present invention, volumes of aqueous ethanol concentration is 55-75%.
Preferably, in Chinese medicine powder, preparation method thereof of the present invention, the temperature of each refluxing extraction is 55-70 DEG C.
Preferably, in Chinese medicine powder, preparation method thereof of the present invention, relative density is concentrated into as 1.12-1.2.
In Chinese medicine powder raw material of the present invention：
Ramulus Taxilli, the stem and branch with leaf of Loranthaceae plant Ramulus Taxilli Taxillus chinensis (DC.) Danser..Taste
It is bitter, sweet, it is mild-natured.Return liver warp, kidney channel.Containing flavone compound, predominantly auicularin, Quercetin and quercitin；Containing gold in leaf
Silk peach glycosides.The ingredients such as Ling Han avicularins, dextrorotation catechol, Quercetin, quercitin.Filling liver kidney, strengthening the bones and muscles, wind-damp dispelling, tocolysis member.
Belong to the wind-damp dispelling strengthening the bones and muscles medicine of medicine for rheumatism subordinate classification.There is decompression, increase coronary blood flow, improve myocardial contractive power effect,
Also diuresis, resisting pathogenic microbes effect, can still inhibit hepatitis type B virus.Ramulus Taxilli water-leach liquor, one water-leach liquor of ethyl alcohol
There is antihypertensive effect to anesthetized animal with 30% alcohol leaching liquid；Parenteral solution has normal and vibration guinea pig heart coronary vasodilator
Diastole acts on.In addition, also having diuresis and an antivirus action.
Eclipta, the dry aerial parts of feverfew Eclipta prostrata Eclipta prostrata L..It is cold in nature, it is sweet in flavor, sour.Return
Kidney channel, Liver Channel.Herb contains saponin 1.32%, nicotine about 0.08%, tannin, vitamin A, ecliptine, a variety of thiophene compounds, leaf
Containing in 2- (diacetylene base) -5- (ethylene acetylene base) thiophene, wedelolactone, demethylwedelolactone, demethyl wedelia chinensis
Ester -7- glycosides, aerial part petroleum ether extraction part contain stigmasterol, phytosterol A and β-botany bar gum；In ethanol extract
Still-the 7-O- containing cyanidenon glycosides, phytosterol A glycosides and a kind of triterpenic acid glycoside.It is nourishing liver and kidney, cool blood
Hemostasis.Belong to the blood-cooling hemostatic of hemostatic subordinate classification.It is grown with inhibiting bacteria；There is apparent protection to make to liver function
With；Enhance body's immunity；There is apparent antimutagenesis；There is hemostasis, improve systema cariovasculare functional；To food
109 cell of pipe cancer has moderate lethal effect；In addition, there is apparent analgesic activity to mouse, carbon tetrachloride can obviously reduce
The increase of the hexobarbital sleeping time of induction and the increase of Zoxazolamine paralysis time.
Common Achyranthes Herb, the herb of amaranthaceous plant achyranthes aspera.It is cold in nature, it is sweet in flavor, light.Distributed in lung channel, bladder warp, Liver Channel.Root, seed contain
Saponin(e, predominantly Common Achyranthes Herb saponin(e；Common Achyranthes Herb saponin(e；.Herb hydroxyl ecdysone；Again containing glycine betaine, back-off potash.Solution
Table heat-clearing, promoting diuresis for stranguria, promoting blood circulation to remove blood stasis.Belong to the stranguria-relieving diuretic of damp-clearing drug subordinate classification, there is inhibition to make to corynebacterium diphtheriae
With, and play the role of neutralizing corynebacterium diphtheriae toxin；Saponin(e contained by seed can substantially strengthen isolated frog heart, the rabbit heart, the cavy heart and
Rabbit contractile force in place；Back-off potash plays the role of dog decompression, reducing heart rate, expansion blood vessel, increase respiratory rate and amplitude.
Rough melic herb, the herb of grass rough melic herb Pogonatherum crinitum (Thunb.) Kunth.It is cool in nature,
It is sweet in flavor.Channel tropism without.Heat-clearing, relieving summer-heat, diuresis.Belong to the edema-alleviating diuretic of damp-clearing drug subordinate classification.
Routine Tilapia mossambica special feed of the present invention can be the production of Tianjin autumn waters -- limid eyes Yu Yue cultural spreadings Co., Ltd
28 albumen tilapia feeds.In conventional method, daily feeding 3-4 times, juvenile fish daily ration of feeding is the 3-5% of weight, and adult fish day is thrown
Bait amount is in the 1-2% of weight.
Compared with prior art, the beneficial effects of the present invention are：
The present invention is improved in the cultural method of Tilapia mossambica oxidation resistance, and Tilapia mossambica special feed is mixed throwing with traditional Chinese medicine powder
Tilapia mossambica is fed, due to Ramulus Taxilli in traditional Chinese medicine powder, eclipta, Common Achyranthes Herb, rough melic herb synergistic effect can significantly improve Tilapia
Matter oxidation resistance promotes the economic benefit of Tilapia mossambica.
Specific embodiment
The invention will be further described with reference to embodiments, but the invention is not limited in these embodiments.
Embodiment 1
The cultural method of Tilapia mossambica oxidation resistance is improved, including following preparation process：Take conventional Tilapia mossambica special feed
200 mesh are crushed to, then are uniformly mixed with 0.12% traditional Chinese medicine powder of conventional Tilapia mossambica special feed weight, are granulated, according to routine
Method carry out feeding Tilapia mossambica.
The traditional Chinese medicine powder is made of the raw material of following parts by weight：10 parts of Ramulus Taxilli, 30 parts of eclipta, 7 parts of Common Achyranthes Herb,
8 parts of rough melic herb.
The Chinese medicine powder, preparation method thereof is：Whole raw materials are weighed by weight, is uniformly mixed, is crushed to 150 mesh, are added in
Whole 30 times of volumetric concentrations of raw material weight are 55% ethanol solution, and three times, the temperature of each refluxing extraction is 70 to refluxing extraction
DEG C, 40min is extracted every time, merges extracting solution, and it is 1.2 to be concentrated into relative density, spray drying.
The antioxidation for the Tilapia mossambica that the cultivation of 1 embodiment 1 of table obtains
Note：The test example part that assay method is seen below.
Embodiment 2
The cultural method of Tilapia mossambica oxidation resistance is improved, including following preparation process：Take conventional Tilapia mossambica special feed
250 mesh are crushed to, then are uniformly mixed with 0.08% traditional Chinese medicine powder of conventional Tilapia mossambica special feed weight, are granulated, according to routine
Method carry out feeding Tilapia mossambica.
The traditional Chinese medicine powder is made of the raw material of following parts by weight：15 parts of Ramulus Taxilli, 22 parts of eclipta, 12 parts of Common Achyranthes Herb,
5 parts of rough melic herb.
The Chinese medicine powder, preparation method thereof is：Whole raw materials are weighed by weight, is uniformly mixed, is crushed to 200 mesh, are added in
Whole 23 times of volumetric concentrations of raw material weight are 65% ethanol solution, and three times, the temperature of each refluxing extraction is 60 to refluxing extraction
DEG C, 50min is extracted every time, merges extracting solution, and it is 1.16 to be concentrated into relative density, spray drying.
The antioxidation for the Tilapia mossambica that the cultivation of 2 embodiment 2 of table obtains
Note：The test example part that assay method is seen below.
Embodiment 3
The cultural method of Tilapia mossambica oxidation resistance is improved, including following preparation process：Take conventional Tilapia mossambica special feed
300 mesh are crushed to, then are uniformly mixed with 0.04% traditional Chinese medicine powder of conventional Tilapia mossambica special feed weight, are granulated, according to routine
Method carry out feeding Tilapia mossambica.
The traditional Chinese medicine powder is made of the raw material of following parts by weight：20 parts of Ramulus Taxilli, 15 parts of eclipta, 17 parts of Common Achyranthes Herb,
2 parts of rough melic herb.
The Chinese medicine powder, preparation method thereof is：Whole raw materials are weighed by weight, is uniformly mixed, is crushed to 250 mesh, are added in
Whole 15 times of volumetric concentrations of raw material weight are 75% ethanol solution, and three times, the temperature of each refluxing extraction is 55 to refluxing extraction
DEG C, 60min is extracted every time, merges extracting solution, and it is 1.12 to be concentrated into relative density, spray drying.
The antioxidation for the Tilapia mossambica that the cultivation of 3 embodiment 3 of table obtains
Note：The test example part that assay method is seen below.
Comparative example 1
The cultural method of Tilapia mossambica oxidation resistance is improved, including following preparation process：Take conventional Tilapia mossambica special feed
250 mesh are crushed to, then are uniformly mixed with 0.08% traditional Chinese medicine powder of conventional Tilapia mossambica special feed weight, are granulated, according to routine
Method carry out feeding Tilapia mossambica.
The traditional Chinese medicine powder is made of the raw material of following parts by weight：22 parts of eclipta, 12 parts of Common Achyranthes Herb, 5 parts of rough melic herb.
The Chinese medicine powder, preparation method thereof is：Whole raw materials are weighed by weight, is uniformly mixed, is crushed to 200 mesh, are added in
Whole 23 times of volumetric concentrations of raw material weight are 65% ethanol solution, and three times, the temperature of each refluxing extraction is 60 to refluxing extraction
DEG C, 50min is extracted every time, merges extracting solution, and it is 1.16 to be concentrated into relative density, spray drying.
The antioxidation for the Tilapia mossambica that the cultivation of 4 comparative example 1 of table obtains
Note：The test example part that assay method is seen below.
Comparative example 2
The cultural method of Tilapia mossambica oxidation resistance is improved, including following preparation process：Take conventional Tilapia mossambica special feed
250 mesh are crushed to, then are uniformly mixed with 0.08% traditional Chinese medicine powder of conventional Tilapia mossambica special feed weight, are granulated, according to routine
Method carry out feeding Tilapia mossambica.
The traditional Chinese medicine powder is made of the raw material of following parts by weight：15 parts of Ramulus Taxilli, 12 parts of Common Achyranthes Herb, 5 parts of rough melic herb.
The Chinese medicine powder, preparation method thereof is：Whole raw materials are weighed by weight, is uniformly mixed, is crushed to 200 mesh, are added in
Whole 23 times of volumetric concentrations of raw material weight are 65% ethanol solution, and three times, the temperature of each refluxing extraction is 60 to refluxing extraction
DEG C, 50min is extracted every time, merges extracting solution, and it is 1.16 to be concentrated into relative density, spray drying.
The antioxidation for the Tilapia mossambica that the cultivation of 5 comparative example 2 of table obtains
Note：The test example part that assay method is seen below.
Comparative example 3
The cultural method of Tilapia mossambica oxidation resistance is improved, including following preparation process：Take conventional Tilapia mossambica special feed
250 mesh are crushed to, then are uniformly mixed with 0.08% traditional Chinese medicine powder of conventional Tilapia mossambica special feed weight, are granulated, according to routine
Method carry out feeding Tilapia mossambica.
The traditional Chinese medicine powder is made of the raw material of following parts by weight：15 parts of Ramulus Taxilli, 22 parts of eclipta, 5 parts of rough melic herb.
The Chinese medicine powder, preparation method thereof is：Whole raw materials are weighed by weight, is uniformly mixed, is crushed to 200 mesh, are added in
Whole 23 times of volumetric concentrations of raw material weight are 65% ethanol solution, and three times, the temperature of each refluxing extraction is 60 to refluxing extraction
DEG C, 50min is extracted every time, merges extracting solution, and it is 1.16 to be concentrated into relative density, spray drying.
The antioxidation for the Tilapia mossambica that the cultivation of 6 comparative example 3 of table obtains
Note：The test example part that assay method is seen below.
Comparative example 4
The cultural method of Tilapia mossambica oxidation resistance is improved, including following preparation process：Take conventional Tilapia mossambica special feed
250 mesh are crushed to, then are uniformly mixed with 0.08% traditional Chinese medicine powder of conventional Tilapia mossambica special feed weight, are granulated, according to routine
Method carry out feeding Tilapia mossambica.
The traditional Chinese medicine powder is made of the raw material of following parts by weight：15 parts of Ramulus Taxilli, 22 parts of eclipta, 12 parts of Common Achyranthes Herb.
The Chinese medicine powder, preparation method thereof is：Whole raw materials are weighed by weight, is uniformly mixed, is crushed to 200 mesh, are added in
Whole 23 times of volumetric concentrations of raw material weight are 65% ethanol solution, and three times, the temperature of each refluxing extraction is 60 to refluxing extraction
DEG C, 50min is extracted every time, merges extracting solution, and it is 1.16 to be concentrated into relative density, spray drying.
The antioxidation for the Tilapia mossambica that the cultivation of 7 comparative example 4 of table obtains
Note：The test example part that assay method is seen below.
Test example：The antioxidation for the Tilapia mossambica that cultural method of the present invention cultivates
1st, material：The Tilapia mossambica that cultural method of the present invention cultivates.
2nd, test method：
(1) Tilapia mossambica runic object prepares extracting method：The Tilapia mossambica 10g that cultivation obtains is taken, is beaten respectively, by solid-liquid ratio 1：
25 extracting in waters, extraction three times, merge extracting solution, are concentrated into certain volume, add 3 times 95% of ethanol precipitation, take supernatant dense
Contracting, drying, it is spare to obtain Tilapia mossambica crude extract.
(2) measure of reducing power：Sample is by the potassium ferricyanide (K3Fe(CN)6) it is reduced into potassium ferrocyanide (K4Fe(CN)6),
Potassium ferrocyanide again with Fe3+Effect, generation ferric ferrocyanide (Prussian blue) are Prussian blue to be detected at 700nm wavelength
Absorbance represents the size of reducing power, and absorbance is higher, and the reducing power of sample is stronger, and inoxidizability is stronger.In 700nm wavelength
Place, measured concentration is the light absorption value of the Tilapia mossambica crude extract of 5mg/ml respectively.
(3) superoxide anion experiment is removed：Generating ultra-oxygen anion free radical (O2-) reaction system in, respectively plus
Enter the Tilapia mossambica crude extract that concentration is 5mg/ml, measure their clearance rates.
(4) experiment of hydroxyl radical free radical is removed：FeSO4With H2O2Reaction generates OH, and salicylic acid is added in system, can
Capture hydroxyl radical free radical simultaneously generate coloring matter, which has absorption maximum at 510nm, when in reaction system there are OH from
During by base scavenger, this oxidation process is suppressed, and absorbance then reduces.In FeSO4With H2O2In reaction system, add respectively
Enter the Tilapia mossambica crude extract that concentration is 30mg/ml, measure clearance rate.
(5) NO under the conditions of simulate the gastric juice pH2-Cleaning reaction acts on：It is effectively to prevent N- nitrosylations to remove nitrite
Close one of carcinogenic approach of object.The Tilapia mossambica crude extract for being separately added into that concentration is 14mg/ml is added in simulated system, is measured clear
Except rate.
(6) DPPH methods measure mushroom antioxidation activity：DPPH is a kind of free radical of stabilization in organic solvent, extensively
For in anti-oxidant appraisement system.In system, the Tilapia mossambica crude extract that concentration is 30mg/ml is separately added into, measures clearance rate.
Claims (7)
1. improve the cultural method of Tilapia mossambica oxidation resistance, which is characterized in that including following preparation process：Take conventional Tilapia mossambica
Special feed is crushed to 200-300 mesh, then is uniformly mixed with traditional Chinese medicine powder, is granulated, conventionally carries out feeding Tilapia mossambica
；
The amount of the traditional Chinese medicine powder is the 0.04-0.12% of conventional Tilapia mossambica special feed weight；
The traditional Chinese medicine powder is made of the raw material of following parts by weight：10-20 parts of Ramulus Taxilli, 15-30 parts of eclipta, Common Achyranthes Herb 7-
17 parts, 2-8 parts of rough melic herb.
2. the cultural method according to claim 1 for improving Tilapia mossambica oxidation resistance, it is characterised in that：The Chinese medicine
The amount of powder is the 0.08% of conventional Tilapia mossambica special feed weight.
3. the cultural method according to claim 1 for improving Tilapia mossambica oxidation resistance, it is characterised in that：The Chinese medicine
Powder is made of the raw material of following parts by weight：15 parts of Ramulus Taxilli, 22 parts of eclipta, 12 parts of Common Achyranthes Herb, 5 parts of rough melic herb.
4. the cultural method of the raising Tilapia mossambica oxidation resistance according to claim 1-3 any one, it is characterised in that：
The Chinese medicine powder, preparation method thereof is：Whole raw materials are weighed by weight, are uniformly mixed, are crushed to 150-250 mesh, are added in all
The ethanol solution of 15-30 times of raw material weight, refluxing extraction three times, extract 40-60min, merge extracting solution, concentrate every time, spraying
It is dry.
5. the cultural method according to claim 4 for improving Tilapia mossambica oxidation resistance, it is characterised in that：The Chinese medicine
In powder, preparation method thereof, volumes of aqueous ethanol concentration is 55-75%.
6. the cultural method according to claim 4 for improving Tilapia mossambica oxidation resistance, it is characterised in that：The Chinese medicine
In powder, preparation method thereof, the temperature of each refluxing extraction is 55-70 DEG C.
7. the cultural method according to claim 4 for improving Tilapia mossambica oxidation resistance, it is characterised in that：The Chinese medicine
In powder, preparation method thereof, relative density is concentrated into as 1.12-1.2.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054973A (en) * | 2014-07-08 | 2014-09-24 | 明光市永言水产食品有限公司 | Feed used for improving immune function of carp |
| CN105076713A (en) * | 2015-09-30 | 2015-11-25 | 通威股份有限公司 | Composite feed capable for improving intestinal health of tilapia and preparation method of composite feed |
| CN105124181A (en) * | 2015-07-28 | 2015-12-09 | 中国水产科学研究院长江水产研究所 | Composite feed additive capable of improving low temperature resistance of tilapia and preparation method thereof |
| CN106551185A (en) * | 2016-11-22 | 2017-04-05 | 防城港市海洋局 | A kind of Chinese medicine feed additive for tilapia and preparation method thereof |
| CN107259085A (en) * | 2017-06-27 | 2017-10-20 | 宁国市仙之居家庭农场 | It is a kind of to improve the Chinese medicine fodder and preparation method of mandarin fish immunity |
-
2017
- 2017-12-26 CN CN201711436924.6A patent/CN108077125A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104054973A (en) * | 2014-07-08 | 2014-09-24 | 明光市永言水产食品有限公司 | Feed used for improving immune function of carp |
| CN105124181A (en) * | 2015-07-28 | 2015-12-09 | 中国水产科学研究院长江水产研究所 | Composite feed additive capable of improving low temperature resistance of tilapia and preparation method thereof |
| CN105076713A (en) * | 2015-09-30 | 2015-11-25 | 通威股份有限公司 | Composite feed capable for improving intestinal health of tilapia and preparation method of composite feed |
| CN106551185A (en) * | 2016-11-22 | 2017-04-05 | 防城港市海洋局 | A kind of Chinese medicine feed additive for tilapia and preparation method thereof |
| CN107259085A (en) * | 2017-06-27 | 2017-10-20 | 宁国市仙之居家庭农场 | It is a kind of to improve the Chinese medicine fodder and preparation method of mandarin fish immunity |
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Application publication date: 20180529 |