CN104256174A - Compound immunity enhancing agent for trachidermus fasciatus heckel and preparation method of compound immunity enhancing agent - Google Patents
Compound immunity enhancing agent for trachidermus fasciatus heckel and preparation method of compound immunity enhancing agent Download PDFInfo
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- CN104256174A CN104256174A CN201410517678.7A CN201410517678A CN104256174A CN 104256174 A CN104256174 A CN 104256174A CN 201410517678 A CN201410517678 A CN 201410517678A CN 104256174 A CN104256174 A CN 104256174A
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Abstract
The invention discloses a compound immunity enhancing agent for trachidermus fasciatus heckle. The compound immunity enhancing agent is prepared from the following components in parts by weight: 20-30 parts of ephedra sinica stapf, 10-15 parts of humifuse euphorbia herb, 5-10 parts of acorus calamus, 10-16 parts of an agrimonia pilosa ledeb extract, 4-8 parts of a galla chinensis extract, 10-20 parts of mulberry leaves, 1-5 parts of verbena officinalis L, 4-6 parts of a pulsatilla chinensis regel extract, 4-10 parts of pericarpium citri reticulatae, 1-3 parts of gardenia jasminoides ellis and 1-3 parts of ferula fukanensis. As an additive, the compound immunity enhancing agent for the trachidermus fasciatus heckle cooperates with a regular feed, so as to replace antibiotics; the compound immunity enhancing agent can prevent and treat all the year and the morbidity is reduced; all the components are used cooperatively so that various diseases including saprolegniasis, gill rot diseases, fixedness infusorian diseases and the like can be effectively prevented and treated; the organism immunity of the trachidermus fasciatus heckle can be enhanced by daily feeding and the resistance to diseases and insect pests is improved; the formula has no toxic or side effect or untoward effect and has no adverse effect on the palatability of the feed; and pericarpium citri reticulatae, gardenia jasminoides ellis and ferula fukanensis are compounded so that the feed calling effect and the effect of improving the palatability of the feed can be realized.
Description
Technical field
The invention belongs to field of feed additive technology, be specifically related to a kind of Sungkiang weever composite immune reinforcing agent and preparation method thereof.
background technology
Sungkiang weever is named again four cheek perches, flower-drum fish etc., and become fish length 5-17cm, health is long spindle, and front is put down flat, the nearly cylindrical shape in rear portion, gradually tiny and sharp backward, and head is large, wide and flat, the full covering skin of women's head-ornaments.Sungkiang weever distributes very wide in the fresh water and shallow sea of China, all distribution is had in the Bohai Sea, the East China Sea and the Yellow Sea bank and rivers and lakes, maritime rivers and creeks, but be main distributed area with Yangtze River Delta, have the habit of breeding migration, annual autumn and winter season cherishes ovum, start from late November to enter sea from freshwater, terminate to early Febuary in next year, last 2 wheat harvesting periods, then lay eggs at neritic province domain March, ovum tool stickiness, can become block to be attached on bivalves ghost or lithocarpus glaber.Sungkiang weever and the Yellow River carp, Song Hua River mandarin fish, Xinkaihu Lake whitefish be called the four your name fishes of China, the tenderly white fertilizer of meat is tender, and boil old for a long time, stingless in meat, taste is extremely delicious, is described as the treasure delicacies of fingerling from ancient times, welcomed by the people.
In recent years, due to the development of water conservancy construction, rivers and lakes are caused to completely cut off, Sungkiang weever is made to lose growth, the place of growing, the juvenile fish resource of recruitment stock is caused constantly to reduce, and agricultural chemicals, the impact environmental pollution that reason causes such as widely using of chemical fertilizer, the natural resources of Sungkiang weever has been made to occur serious exhausted, supply falls short of demand in market, propagating artificially of Sungkiang weever is impelled to develop rapidly, at present, Sungkiang weever often can be subject to saprolegniasis in breeding process, bacterial gill, trichodinasis, sedentarius ciliate is sick, white skin disease, the invasion of the multiple disease and pests such as gastroenteritis is sick, affect the speed of growth of Sungkiang weever, and survival rate declines, have a strong impact on the economic benefit of cultivation, for disease and pest, raiser adopts chemical agent to kill mostly, chemical reagent is killed, although certain effect can be played, exist residual, even harmful components can enter in fish body, and then be detrimental to health.How to prevent before disease and pest occurs, improve the invasion of fish body opposing disease and pest, reduce the incidence of disease, become crucial.In the breeding process of fish, throwing something and feeding of feed is a step of most critical, the raising of fish body immunity, also closely related with throwing something and feeding of feed, therefore, in feed, add composite immune reinforcing agent, being regulated body health by feed of throwing something and feeding, to strengthen immunity of organisms, restraining and sterilizing bacteria pest-resistant, to Sungkiang weever propagate artificially extremely important.
Summary of the invention
For problems of the prior art, the object of the invention is to provide a kind of Sungkiang weever composite immune reinforcing agent and preparation method thereof.
The present invention is realized by the following technical programs:
Described Sungkiang weever composite immune reinforcing agent, is characterized in that this composite immune reinforcing agent is made up of the component of following parts by weight: Chinese ephedra 20-30 part, wolf's milk 10-15 part, calamus 5-10 part, Herba Agrimoniae extract 10-16 part, nutgall extractive 4-8 part, mulberry leaves 10-20 part, Verbena officinalis 1-5 part, Radix Pulsatillae extract 4-6 part, dried orange peel 4-10 part, cape jasmine 1-3 part, asafoetide 1-3 part.
Described Sungkiang weever composite immune reinforcing agent, is characterized in that being made up of the component of following parts by weight: 25 parts, Chinese ephedra, wolf's milk 13 parts, calamus 7 parts, Herba Agrimoniae extract 13 parts, nutgall extractive 6 parts, mulberry leaves 15 parts, Verbena officinalis 3 parts, Radix Pulsatillae extract 5 parts, dried orange peel 7 parts, cape jasmine 2 parts, asafoetide 2 parts.
Described Sungkiang weever composite immune reinforcing agent, is characterized in that this composite immune reinforcing agent adds in Sungkiang weever mixed feed, and the usage ratio of composite immune reinforcing agent and Sungkiang weever mixed feed is 1:70-100.
Described Sungkiang weever composite immune reinforcing agent, is characterized in that described composite immune reinforcing agent adds in Sungkiang weever mixed feed, and the usage ratio of composite immune reinforcing agent and Sungkiang weever mixed feed is 1:85.
The preparation method of described Sungkiang weever composite immune reinforcing agent, is characterized in that following these steps to carry out:
1) take the mulberry leaves of formula ratio, Verbena officinalis, dried orange peel, cape jasmine, asafoetide respectively, dry after cleaning up in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 mesh sieves, obtains each composition dry powder, for subsequent use;
2) by Chinese ephedra stalk disintegrating, add the soak by water of ten times amount, continue to boil 2-3 minute after boiling, filter, filtrate is concentrated into 5% of original volume, obtains Chinese gall Aqueous extracts, for subsequent use;
3), after wolf's milk, calamus being cleaned, squeeze the juice, obtain wolf's milk juice, calamus juice respectively, for subsequent use;
4), after getting the obtained each composition dry powder blend of formula ratio Chinese gall Aqueous extracts, wolf's milk juice, calamus juice and step 1) respectively, Herba Agrimoniae extract, nutgall extractive, the Radix Pulsatillae extract of formula ratio is added, mixing and stirring.
The preparation method of described Sungkiang weever composite immune reinforcing agent, it is characterized in that the preparation method of described Herba Agrimoniae extract is: add in weight 10-20 95% ethanolic solution doubly after being pulverized by hairyvein agrimony herb, refluxing extraction, each extraction 1-2 hour, repeat to extract 2-3 time, extract is concentrated into medicinal extract, and medicinal extract spraying dry under the temperature conditions of 165 DEG C, obtains Herba Agrimoniae extract.
The preparation method of described Sungkiang weever composite immune reinforcing agent, it is characterized in that the preparation method of described nutgall extractive is: after Chinese gall is pulverized, add 3-5 doubly to Chinese gall weight 90% alcohol steep 6-8 hour, leaching liquor concentration, to medicinal extract, obtains nutgall extractive.
The preparation method of described Sungkiang weever composite immune reinforcing agent, is characterized in that described Radix Pulsatillae extract is pulverized, and adds 5-6 water doubly by weight, boils 1 hour, filter, be concentrated into medicinal extract, obtain Radix Pulsatillae extract.
Compared with existing preparation, the present invention has the following advantages:
1) as additive and Sungkiang weever conventional feed with the use of, substitute antibiotics, annual control, reduces the incidence of disease, uses safer;
2) Chinese ephedra has the effect of water prevention mildew; Wolf's milk and calamus are with the use of effectively preventing and treating fin rot; Herba Agrimoniae extract has the effect of preventing and treating gastroenteritis disease;
3) nutgall extractive and mulberry leaves with the use of, can white skin disease be prevented and treated, play the effect of antibacterial sterilizing; Verbena officinalis and Radix Pulsatillae extract are with the use of playing to trichodinasis and sedentarius ciliate disease the effect killed;
4) cooperation of each composition, can strengthen Sungkiang weever immunity of organisms, improves the resistance to disease and pest;
5) to have no side effect and bad reaction, the palatability for feed has no adverse effects, and dried orange peel, cape jasmine and asafoetide are composite, can play food calling and improve the effect of palatability of feed.
Detailed description of the invention
By following specific embodiment, the invention will be further described.
Embodiment 1
Sungkiang weever composite immune reinforcing agent, consists of: 25 parts, Chinese ephedra, wolf's milk 13 parts, calamus 7 parts, Herba Agrimoniae extract 13 parts, nutgall extractive 6 parts, mulberry leaves 15 parts, Verbena officinalis 3 parts, Radix Pulsatillae extract 5 parts, dried orange peel 7 parts, cape jasmine 2 parts, asafoetide 2 parts.Its preparation method is: take the mulberry leaves of formula ratio, Verbena officinalis, dried orange peel, cape jasmine, asafoetide respectively, dry after cleaning up in drying baker, and after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 mesh sieves, obtains each composition dry powder, for subsequent use; By Chinese ephedra stalk disintegrating, add the soak by water of ten times amount, continue to boil 2-3 minute after boiling, filter, filtrate is concentrated into 5% of original volume, obtains Chinese gall Aqueous extracts, for subsequent use; After wolf's milk, calamus are cleaned, squeeze the juice, obtain wolf's milk juice, calamus juice respectively, for subsequent use; After getting the obtained each composition dry powder blend of formula ratio Chinese gall Aqueous extracts, wolf's milk juice, calamus juice and step 1) respectively, add Herba Agrimoniae extract, nutgall extractive, the Radix Pulsatillae extract of formula ratio, namely mixing and stirring obtains Sungkiang weever composite immune reinforcing agent.Get the composite immune reinforcing agent 1 part prepared, mix with 85 parts of Sungkiang weever conventional feeds, Sungkiang weever is fed.
Wherein, the preparation method of Herba Agrimoniae extract is: add in weight 10-20 95% ethanolic solution doubly after being pulverized by hairyvein agrimony herb, refluxing extraction, each extraction 1-2 hour, repeat to extract 2-3 time, extract is concentrated into medicinal extract, and medicinal extract spraying dry under the temperature conditions of 165 DEG C, obtains Herba Agrimoniae extract.
The preparation method of nutgall extractive is: after Chinese gall is pulverized, add 3-5 doubly to Chinese gall weight 90% alcohol steep 6-8 hour, leaching liquor concentration, to medicinal extract, obtains nutgall extractive.
The preparation method of Radix Pulsatillae extract, pulverizes the Chinese bulbul, adds 5-6 water doubly by weight, boils 1 hour, filter, be concentrated into medicinal extract, obtain Radix Pulsatillae extract.
Embodiment 2
Sungkiang weever composite immune reinforcing agent, consists of: 30 parts, Chinese ephedra, wolf's milk 10 parts, calamus 10 parts, Herba Agrimoniae extract 10 parts, nutgall extractive 8 parts, mulberry leaves 10 parts, Verbena officinalis 5 parts, Radix Pulsatillae extract 4 parts, dried orange peel 10 parts, cape jasmine 1 part, asafoetide 3 parts.Its preparation method is: take the mulberry leaves of formula ratio, Verbena officinalis, dried orange peel, cape jasmine, asafoetide respectively, dry after cleaning up in drying baker, and after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 mesh sieves, obtains each composition dry powder, for subsequent use; By Chinese ephedra stalk disintegrating, add the soak by water of ten times amount, continue to boil 2-3 minute after boiling, filter, filtrate is concentrated into 5% of original volume, obtains Chinese gall Aqueous extracts, for subsequent use; After wolf's milk, calamus are cleaned, squeeze the juice, obtain wolf's milk juice, calamus juice respectively, for subsequent use; After getting the obtained each composition dry powder blend of formula ratio Chinese gall Aqueous extracts, wolf's milk juice, calamus juice and step 1) respectively, add Herba Agrimoniae extract, nutgall extractive, the Radix Pulsatillae extract of formula ratio, namely mixing and stirring obtains Sungkiang weever composite immune reinforcing agent.Get the composite immune reinforcing agent 1 part prepared, mix with 70 parts of Sungkiang weever conventional feeds, Sungkiang weever is fed.
The extracting method of Herba Agrimoniae extract, nutgall extractive, Radix Pulsatillae extract is with embodiment 1.
Embodiment 3
Sungkiang weever composite immune reinforcing agent, consists of: 20 parts, Chinese ephedra, wolf's milk 15 parts, calamus 5 parts, Herba Agrimoniae extract 16 parts, nutgall extractive 4 parts, mulberry leaves 20 parts, Verbena officinalis 1 part, Radix Pulsatillae extract 6 parts, dried orange peel 4 parts, cape jasmine 3 parts, asafoetide 1 part.Its preparation method is: take the mulberry leaves of formula ratio, Verbena officinalis, dried orange peel, cape jasmine, asafoetide respectively, dry after cleaning up in drying baker, and after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 mesh sieves, obtains each composition dry powder, for subsequent use; By Chinese ephedra stalk disintegrating, add the soak by water of ten times amount, continue to boil 2-3 minute after boiling, filter, filtrate is concentrated into 5% of original volume, obtains Chinese gall Aqueous extracts, for subsequent use; After wolf's milk, calamus are cleaned, squeeze the juice, obtain wolf's milk juice, calamus juice respectively, for subsequent use; After getting the obtained each composition dry powder blend of formula ratio Chinese gall Aqueous extracts, wolf's milk juice, calamus juice and step 1) respectively, add Herba Agrimoniae extract, nutgall extractive, the Radix Pulsatillae extract of formula ratio, namely mixing and stirring obtains Sungkiang weever composite immune reinforcing agent.Get the composite immune reinforcing agent 1 part prepared, mix with 100 parts of Sungkiang weever conventional feeds, Sungkiang weever is fed.
The extracting method of Herba Agrimoniae extract, nutgall extractive, Radix Pulsatillae extract is with embodiment 1.
Control group 1
Sungkiang weever composite immune reinforcing agent, consists of: 15 parts, Chinese ephedra, wolf's milk 20 parts, calamus 1 part, Herba Agrimoniae extract 17 parts, nutgall extractive 3 parts, mulberry leaves 2 parts, Verbena officinalis 6 parts, Radix Pulsatillae extract 2 parts, dried orange peel 14 parts, cape jasmine 4 parts, asafoetide 0.5 part.Preparation method is with embodiment 1.Get Sungkiang weever composite immune reinforcing agent 1 part, mix with the Sungkiang weever conventional feed of 70 parts, Sungkiang weever is thrown something and fed.
Control group 2
Adopt and throw something and feed with the conventional feed that embodiment 1 is identical, do not add composite immune reinforcing agent.
Test example
Pond is separated into five regions of the same area, be numbered 1,2,3,4,5, at the beginning of 5 months, throw in long loach seedling 200 tail for about 2cm of body respectively to each marker space, wherein, 1 throws something and feeds according to the formulation content of embodiment 1, and 2 throw something and feed according to the formulation content of embodiment 2, and 3 throw something and feed according to the formulation content of embodiment 3,4 throw something and feed according to the formulation content of control group, 5 throw something and feed according to control group 2 formulation content, and feed 7 months, feeding effect is in table 1.
Table 1 feeding effect
Experimental result shows: when external condition is identical, and Sungkiang weever composite immune reinforcing agent of the present invention can significantly improve growth rate and the body weight of Sungkiang weever, reduces the incidence of disease of Sungkiang weever, improves survival rate, reduces feed coefficient.As seen from Table 1; the Sungkiang weever of No. 1 isolated area adopting embodiment 1 content formula to throw something and feed; indices is all better than other each group; the Sungkiang weever of No. 4 isolated areas adopting control group 1 content formula to throw something and feed; because the content of each composition of control group 1 is not all in the protection domain of present patent application, its indices of feeding is all lower than the feeding effect of embodiment 1-3.
Claims (8)
1. Sungkiang weever composite immune reinforcing agent, is characterized in that this composite immune reinforcing agent is made up of the component of following parts by weight: Chinese ephedra 20-30 part, wolf's milk 10-15 part, calamus 5-10 part, Herba Agrimoniae extract 10-16 part, nutgall extractive 4-8 part, mulberry leaves 10-20 part, Verbena officinalis 1-5 part, Radix Pulsatillae extract 4-6 part, dried orange peel 4-10 part, cape jasmine 1-3 part, asafoetide 1-3 part.
2. Sungkiang weever composite immune reinforcing agent according to claim 1, is characterized in that being made up of the component of following parts by weight: 25 parts, Chinese ephedra, wolf's milk 13 parts, calamus 7 parts, Herba Agrimoniae extract 13 parts, nutgall extractive 6 parts, mulberry leaves 15 parts, Verbena officinalis 3 parts, Radix Pulsatillae extract 5 parts, dried orange peel 7 parts, cape jasmine 2 parts, asafoetide 2 parts.
3. Sungkiang weever composite immune reinforcing agent according to claim 1 and 2, is characterized in that this composite immune reinforcing agent adds in Sungkiang weever mixed feed, and the usage ratio of composite immune reinforcing agent and Sungkiang weever mixed feed is 1:70-100.
4. Sungkiang weever composite immune reinforcing agent according to claim 4, is characterized in that described composite immune reinforcing agent adds in Sungkiang weever mixed feed, and the usage ratio of composite immune reinforcing agent and Sungkiang weever mixed feed is 1:85.
5. the preparation method of Sungkiang weever composite immune reinforcing agent, is characterized in that following these steps to carry out:
1) take the mulberry leaves of formula ratio, Verbena officinalis, dried orange peel, cape jasmine, asafoetide respectively, dry after cleaning up in drying baker, after drying, each composition is pulverized respectively, wears into fine powder, crosses 40 mesh sieves, obtains each composition dry powder, for subsequent use;
2) by Chinese ephedra stalk disintegrating, add the soak by water of ten times amount, continue to boil 2-3 minute after boiling, filter, filtrate is concentrated into 5% of original volume, obtains Chinese gall Aqueous extracts, for subsequent use;
3), after wolf's milk, calamus being cleaned, squeeze the juice, obtain wolf's milk juice, calamus juice respectively, for subsequent use;
4), after getting the obtained each composition dry powder blend of formula ratio Chinese gall Aqueous extracts, wolf's milk juice, calamus juice and step 1) respectively, Herba Agrimoniae extract, nutgall extractive, the Radix Pulsatillae extract of formula ratio is added, mixing and stirring.
6. the preparation method of Sungkiang weever composite immune reinforcing agent according to claim 5, it is characterized in that the preparation method of described Herba Agrimoniae extract is: add in weight 10-20 95% ethanolic solution doubly after being pulverized by hairyvein agrimony herb, refluxing extraction, each extraction 1-2 hour, repeat to extract 2-3 time, extract is concentrated into medicinal extract, and medicinal extract spraying dry under the temperature conditions of 165 DEG C, obtains Herba Agrimoniae extract.
7. the preparation method of Sungkiang weever composite immune reinforcing agent according to claim 5, it is characterized in that the preparation method of described nutgall extractive is: after Chinese gall is pulverized, add 3-5 doubly to Chinese gall weight 90% alcohol steep 6-8 hour, leaching liquor concentration, to medicinal extract, obtains nutgall extractive.
8. the preparation method of Sungkiang weever composite immune reinforcing agent according to claim 5, is characterized in that described Radix Pulsatillae extract is pulverized, and adds 5-6 water doubly by weight, boils 1 hour, filter, be concentrated into medicinal extract, obtain Radix Pulsatillae extract.
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| KR20190142564A (en) * | 2018-06-18 | 2019-12-27 | 대한민국(농촌진흥청장) | Feed composition comprising melberry leaf for preventing or improving infection of Allomyrina dichotoma Nudivirus |
| CN112385752A (en) * | 2020-11-17 | 2021-02-23 | 广东顺德鼎一生物科技有限公司 | Expanded feed for improving ingestion and growth performance of micropterus salmoides and preparation method thereof |
| CN112493376A (en) * | 2020-11-27 | 2021-03-16 | 黔东南聚龙潭生态渔业有限公司 | Fish feed and method for improving survival rate of fish in long-distance transportation |
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| CN112385752A (en) * | 2020-11-17 | 2021-02-23 | 广东顺德鼎一生物科技有限公司 | Expanded feed for improving ingestion and growth performance of micropterus salmoides and preparation method thereof |
| CN112493376A (en) * | 2020-11-27 | 2021-03-16 | 黔东南聚龙潭生态渔业有限公司 | Fish feed and method for improving survival rate of fish in long-distance transportation |
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Application publication date: 20150107 |
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