CN101700105A - Microecological feed additive for milk cows and preparation method thereof - Google Patents
Microecological feed additive for milk cows and preparation method thereof Download PDFInfo
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- CN101700105A CN101700105A CN200910172675A CN200910172675A CN101700105A CN 101700105 A CN101700105 A CN 101700105A CN 200910172675 A CN200910172675 A CN 200910172675A CN 200910172675 A CN200910172675 A CN 200910172675A CN 101700105 A CN101700105 A CN 101700105A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention belongs to the technical field of microecological feed additive application and relates to a microecological feed additive for milk cows and a preparation method thereof, in particular to a microecological feed additive capable of improving the metabolism of the environment in a milk cow organism and improving the nutritive values of milk products and a preparation method thereof. The preparation method is mainly characterized by comprising the following steps of: firstly carrying out anaerobic fermentation on raw materials such as lactic acid bacillus, corynebacterium glutamicum, bifidobacterium, lactobacillus plantarum, nutrient medium, sterile water, and the like; then respectively centrifuging fermentation liquid and mixing; uniformly mixing with a special nutrition carrier and then placing into a closed fermentation room for anaerobic fermentation; and finally drying, crushing and obtaining a finished product. The microecological feed additive contains a large amount of beneficial microorganisms, antioxidants such as coenzyme, SOD, vitamins, and the like, and physiological metabolites such as amino acid, propylene glycol, active peptide, and the like which can play roles in nourishing cells, restoring damaged cells and promoting cell metabolism after entering into the milk cow organism, constructs fine microecological environments in the organisms of the milk cows, promotes the absorption and the transformation of nutrient substances, improves the immunity of the organisms, increases the milk production of the milk cows, prevent common frequently-occurring diseases such as bovine mastitis and the like and improves the disease-resistant capacity and the quality of the milk products.
Description
1. technical field
The present invention relates to the microecological feed additive for milk cows technical field, specifically microecological feed additive for milk cows and preparation method.After the product that utilizes this method to make enters in the milk cow body; can play the effect of vegetative cell and reparation damaged cell, promotion cellular metabolism; make up the good micro-ecological environment in the milk cow body; promote the absorption of nutriment to transform, improve immunity of organisms; when improving milk production of cow; also some common multiple diseases such as milk cow ketosis and mastitis be can prevent, resistance against diseases and milk products quality improved.
2. background technology
A large amount of microorganism survival and reproductions is arranged in cow rumen and the alimentary canal, can be divided into profitable strain according to its secretion behavior, pernicious bacteria and marginal facultative flora, be mutual restriction between the microbiologic population in cud and the alimentary canal, when wherein a part of group takes up space advantage in enteron aisle, a part of in addition flora then is in a disadvantageous position, at this moment the population equilibrium of microorganism is just destroyed in the alimentary canal, if deflection pernicious bacteria, then can cause the disorder of milk cow digestive system function, bring out disease of digestive tract.Generally, the milk cow outward appearance be it seems very healthy, and the overwhelming majority is in sub-health state in fact, that is to say, when external condition changed, the milk cow resistivity reduced, and disease will occur.Therefore,,, utilize normal microflora member or its to promote the appearance of little ecological system that material is made, be historical certainty, and at home and abroad emerge rapidly in recent years in order to adjust microecological balance according to the microecology principle.
3. summary of the invention
The present invention mainly provides a kind of microecological feed additive for milk cows and preparation method; the product that utilizes this method to make can effectively improve milk cow body micro-ecological environment; improve the quality of the output of milk and dairy produce; and can prevent some common diseases in the milk cow production process, to reach the purpose that improves the animal husbandry economy benefit.Its key character is: some good probioticses such as the Bacillus acidi lactici of utilization screening, Bifidobacterium, corynebacterium glutamicum ferment, some enzymes that produce in its metabolic process can be transformed into the material that adds in the culture medium to the favourable material of milk bovine lactation, can improve the organismic internal environment of milk cow after some metabolites that produce in the fermentation such as amino acid, SOD, active peptides, propane diols etc. enter in the milk cow body simultaneously, and can prevent some common cow diseases such as milk cow ketosis, mastitis etc., can significantly strengthen the immunity of milk cow, promote its lactation.
In order to realize above purpose, a kind of microecological feed additive for milk cows provided by the invention and preparation method is characterized in that: prepared by following weight percentages earlier before the anaerobic fermentation: bacterial classification stoste 4～16%, culture medium A 11%, carrier 51.6%; Described bacterial classification stoste is Bacillus acidi lactici, corynebacterium glutamicum, Bifidobacterium, Lactobacillus plantarum composition, presses the bacterial classification configuration of following percentage by weight: Bacillus acidi lactici 22～43%, corynebacterium glutamicum 16.4%, Bifidobacterium 0.20%, Lactobacillus plantarum 21.45%.Described culture medium is the mixture of soya-bean cake powder, starch, dusty yeast, corn steep liquor, corn flour, molasses, sulfate of ammoniac, sodium hydrogen phosphate, magnesium sulfate, sterilized water formation, and its weight percent proportioning is: soya-bean cake powder 1.5%, starch 30%, corn flour 2.40%, starch 0.15%, dusty yeast 0.10%, sulfate of ammoniac 0.5%, corn steep liquor 0.5%, molasses 0.15%, sodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.3%, water 10.95%.Described nutrition carrier is the mixture of calcium carbonate, bran powder, albumen powder, mealy potato, and its weight percent proportioning is: calcium carbonate 10.309%, bran powder 60.859%, albumen powder 3.10%, mealy potato 21.50%.
Described bacterial classification is selected by the optimization of strictness; Adopt anaerobic fermentation and low temperature cultured method simultaneously, it comprises the following steps:
The first step: utilize chemical mutagen or physical methods such as ultraviolet ray, ionising radiation to carry out mutagenesis Bacillus acidi lactici, corynebacterium glutamicum, Bifidobacterium, Lactobacillus plantarum respectively, and therefrom select good type;
Second step: in the beef-protein medium of respectively above-mentioned acquisition bacterial classification being transferred, after cultivating 24 hours under 35～40 ℃ of temperature, measure its activity and tire, select the bacterial classification that growth is fast, the quantity in beef-protein medium reaches more than 4,000,000,000/ml to preserve;
The 3rd step: bacterial classification inoculation to being equipped with in the nutrient agar test tube, was cultivated 42～50 hours under 35～40 ℃ of temperature, put into 4 ℃ refrigerator then and preserve;
The 4th step: bacterial classification inoculation in through in the culture medium of steam sterilizing, is put into mixer and stirred, and speed of agitator is 140r/min～160r/min, and cultivation temperature is 35～40 ℃, throughput 1: 0.5, and fermentation time is 24 hours, obtains composite bacteria agent capable stoste;
The 5th step: composite bacteria agent capable stoste is added in culture medium and the nutrition carrier according to special ratios, mix, insert airtight container, put between ferment at constant temperature, cultivation temperature is 30～35 ℃, and fermentation time is 14～16 days;
The 6th step: the tunning that obtains is put into low temperature incubator, be 20～30 ℃ in temperature and continue to cultivate 65～75 hours down;
The 7th goes on foot: after the product that obtains is carried out pulverization process, promptly obtain pulverous microbial forage additive.
4. the specific embodiment
The bacterial classification that the preparation method of microecological feed additive for milk cows adopts all comes by screening and optimizing, adopts the low temperature cultured method simultaneously, and it comprises the following steps:
The first step: respectively Bacillus acidi lactici, corynebacterium glutamicum, Bifidobacterium, Lactobacillus plantarum are utilized chemical mutagen or physical methods such as ultraviolet ray, ionising radiation to carry out mutagenesis and carry out preferably;
Second step: in the beef-protein medium of respectively above-mentioned acquisition bacterial classification being transferred, after cultivating 24 hours under 35～40 ℃ of temperature, measure its activity and tire, select the bacterial classification that growth is fast, the quantity in beef-protein medium reaches more than 4,000,000,000/ml to preserve;
The 3rd step: bacterial classification inoculation to being equipped with in the nutrient agar test tube, was cultivated 42～50 hours under 35～40 ℃ of temperature, put into 4 ℃ refrigerator then and preserve;
The 4th step: bacterial classification inoculation in through in the culture medium of steam sterilizing, is put into mixer and stirred, and speed of agitator is 140r/min～160r/min, and cultivation temperature is 35～40 ℃, throughput 1: 0.5, and fermentation time is 24 hours, obtains composite bacteria agent capable stoste;
The 5th step: composite bacteria agent capable stoste is added in culture medium and the nutrition carrier according to special ratios, culture medium is the mixture of soya-bean cake powder, starch, dusty yeast, corn steep liquor, corn flour, molasses, sulfate of ammoniac, sodium hydrogen phosphate, magnesium sulfate, sterilized water formation, and its weight percent proportioning is: soya-bean cake powder 1.5%, starch 30%, corn flour 2.40%, starch 0.15%, dusty yeast 0.10%, sulfate of ammoniac 0.5%, corn steep liquor 0.5%, molasses 0.15%, sodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.3%, water 10.95%.Nutrition carrier is the mixture of calcium carbonate, bran powder, albumen powder, mealy potato, and its weight percent proportioning is: calcium carbonate 10.309%, bran powder 60.859%, albumen powder 3.10%, mealy potato 21.50%.Mix, insert airtight container, put between ferment at constant temperature, cultivation temperature is 30～35 ℃, and fermentation time is 14～16 days;
The 6th step: the tunning that obtains is put into low temperature incubator, be 20～30 ℃ in temperature and continue to cultivate 65～75 hours down.
The 7th goes on foot: after the product that obtains is carried out pulverization process, promptly obtain pulverous microbial forage additive finished product.
Claims (4)
1. microecological feed additive for milk cows of the present invention and preparation method, its key character is: prepared by following weight percentages earlier before the anaerobic fermentation: bacterial classification stoste 4-16%, culture medium A 11%, carrier 51.6%; Described bacterial classification stoste is Bacillus acidi lactici, corynebacterium glutamicum, Bifidobacterium, Lactobacillus plantarum composition, presses the bacterial classification configuration of following percentage by weight: Bacillus acidi lactici 22-43%, corynebacterium glutamicum 16.4%, Bifidobacterium 0.20%, Lactobacillus plantarum 21.45%.
2. microecological feed additive for milk cows according to claim 1 and preparation method, it is characterized in that: described culture medium is the mixture of soya-bean cake powder, starch, dusty yeast, corn steep liquor, corn flour, molasses, sulfate of ammoniac, sodium hydrogen phosphate, magnesium sulfate, sterilized water formation, and its weight percent proportioning is: soya-bean cake powder 1.5%, starch 30%, corn flour 2.40%, starch 0.15%, dusty yeast 0.10%, sulfate of ammoniac 0.5%, corn steep liquor 0.5%, molasses 0.15%, sodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.3%, water 10.95%.
3. microecological feed additive for milk cows according to claim 1 and preparation method, it is characterized in that: described nutrition carrier is the mixture of calcium carbonate, bran powder, albumen powder, mealy potato, and its weight percent proportioning is: calcium carbonate 10.309%, bran powder 60.859%, albumen powder 3.10%, mealy potato 21.50%.
4. microecological feed additive for milk cows according to claim 1 and preparation method is characterized in that: the bacterial classification that is adopted is selected by the optimization of strictness; Adopt anaerobic fermentation and low temperature cultured method simultaneously, it comprises the following steps:
The first step: utilize chemical mutagen or physical methods such as ultraviolet ray, ionising radiation to carry out mutagenesis Bacillus acidi lactici, corynebacterium glutamicum, Bifidobacterium, Lactobacillus plantarum respectively, and therefrom select good type;
Second step: in the beef-protein medium of respectively above-mentioned acquisition bacterial classification being transferred, after cultivating 24 hours under the 35-40 ℃ of temperature, measure its activity and tire, select the bacterial classification that growth is fast, the quantity in beef-protein medium reaches more than 4,000,000,000/ml to preserve;
The 3rd step: bacterial classification inoculation to being equipped with in the nutrient agar test tube, was cultivated 42-50 hour under 35-40 ℃ of temperature, put into 4 ℃ refrigerator then and preserve;
The 4th step: bacterial classification inoculation in through in the culture medium of steam sterilizing, is put into mixer and stirred, and speed of agitator is 140r/min-160r/min, and cultivation temperature is 35-40 ℃, throughput 1: 0.5, and fermentation time is 24 hours, obtains composite bacteria agent capable stoste;
The 5th step: composite bacteria agent capable stoste is added in culture medium and the nutrition carrier according to special ratios, mix, insert airtight container, put between ferment at constant temperature, cultivation temperature is 30-35 ℃, and fermentation time is 14-16 days;
The 6th step: the tunning that obtains is put into low temperature incubator, be 20-30 ℃ in temperature and continue to cultivate 65～75 hours down;
The 7th goes on foot: after the product that obtains is carried out pulverization process, promptly obtain pulverous microbial forage additive.
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| CN200910172675A CN101700105A (en) | 2009-11-24 | 2009-11-24 | Microecological feed additive for milk cows and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550857A (en) * | 2011-12-19 | 2012-07-11 | 北京科兴大地饲料有限公司 | Functional feed for preventing and treating mastitis in dairy cows and preparation method thereof |
| CN104498454A (en) * | 2014-11-28 | 2015-04-08 | 镇江拜因诺生物科技有限公司 | Compound microbial starter culture |
| CN105519823A (en) * | 2016-01-21 | 2016-04-27 | 厦门蓝鳍海洋生物科技有限公司 | Special fly maggot immune feed for aquatic products and preparation method thereof |
| CN105748534A (en) * | 2016-04-29 | 2016-07-13 | 内蒙古和美科盛生物技术有限公司 | Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem |
| CN106578450A (en) * | 2016-11-25 | 2017-04-26 | 南宁学院 | Micro-ecologic preparation for improving immunity of calves, and preparation method and application thereof |
| CN111387348A (en) * | 2020-05-07 | 2020-07-10 | 北京九州大地生物技术集团股份有限公司 | Feed for improving milk yield of dairy cows and preparation method and application thereof |
| CN111418709A (en) * | 2020-05-07 | 2020-07-17 | 北京九州大地生物技术集团股份有限公司 | Feed for preventing dairy cow mastitis and preparation method thereof |
-
2009
- 2009-11-24 CN CN200910172675A patent/CN101700105A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550857A (en) * | 2011-12-19 | 2012-07-11 | 北京科兴大地饲料有限公司 | Functional feed for preventing and treating mastitis in dairy cows and preparation method thereof |
| CN102550857B (en) * | 2011-12-19 | 2013-08-07 | 北京科兴大地饲料有限公司 | Functional feed for preventing and treating mastitis in dairy cows and preparation method thereof |
| CN104498454A (en) * | 2014-11-28 | 2015-04-08 | 镇江拜因诺生物科技有限公司 | Compound microbial starter culture |
| CN105519823A (en) * | 2016-01-21 | 2016-04-27 | 厦门蓝鳍海洋生物科技有限公司 | Special fly maggot immune feed for aquatic products and preparation method thereof |
| CN105748534A (en) * | 2016-04-29 | 2016-07-13 | 内蒙古和美科盛生物技术有限公司 | Composite lactobacillus nipple cleaning solution for improving dairy cow nipple micro-ecosystem |
| CN105748534B (en) * | 2016-04-29 | 2018-12-18 | 内蒙古和美科盛生物技术有限公司 | A kind of compound lactobacillus nipple cleaning solution improving mammilla of milk cattle microecosystem |
| CN106578450A (en) * | 2016-11-25 | 2017-04-26 | 南宁学院 | Micro-ecologic preparation for improving immunity of calves, and preparation method and application thereof |
| CN111387348A (en) * | 2020-05-07 | 2020-07-10 | 北京九州大地生物技术集团股份有限公司 | Feed for improving milk yield of dairy cows and preparation method and application thereof |
| CN111418709A (en) * | 2020-05-07 | 2020-07-17 | 北京九州大地生物技术集团股份有限公司 | Feed for preventing dairy cow mastitis and preparation method thereof |
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Application publication date: 20100505 |