CN106578450A - Micro-ecologic preparation for improving immunity of calves, and preparation method and application thereof - Google Patents
Micro-ecologic preparation for improving immunity of calves, and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of micro-ecologic, and especially provides a micro-ecologic preparation for improving immunity of calves, and a preparation method thereof. The preparation method includes the steps of: preparing a mixture bacterial liquid from a bacillus subtilis natto fermentation liquid, a bacillus licheniformis fermentation liquid, a lactobacillus plantarum fermentation liquid and a saccharomyces cerevisae Hansen fermentation liquid according to the volume ratio of 0.8-1.5:1-1.5:0.6-1.4:0.6-1.3, and adsorbing the mixture bacterial liquid by mean of a carrier, wherein weight ratio of the carrier to the mixture bacterial liquid is 1:1.5. The micro-ecologic preparation is added to calf feed for feeding the calves, so that immunity of the calves is improved and disease rate of the calves is reduced. In addition, the calves grow fast and feed conversion ratio is reduced.
Description
Technical field
The present invention relates to microbial ecological agent technical field, especially a kind of microbial ecological agent and system for improving calf immunity
Preparation Method and application.
Background technology
Calf body weight is little, and body constitution is poor, and immune system needs to be developed, lower for the resistance, and many small unfavorable factors all can
Cause morbidity.Currently, in terms of calf disease resistance, antibiotic is used mostly and is noted as feed additive or directly
Penetrate, to reach calf immunity is strengthened, reduce the sickness rate of calf.But, with the continuous variation of pathogenic microorganism, antibiotic
The limitation of presence and the harm for being brought gradually are exposed to be come, such as：The use of substantial amounts of antibiotic, not only causes calf to exist
Drug resistance in growth course strengthens, and resistance is gradually low, so that the residual quantity in beef and beef product
It is higher, threat is caused to the health of the mankind.It is reported that, have begun in human body and with the mankind eat in most of Europe country
In the animal body that thing chain is directly associated, antibiotic, such as Britain are have disabled.
With progressively projecting for antibiotic defect, have become for a kind of product for being capable of substitute antibiotics effect is studied
It is badly in need of the technical problem for solving.Find that the great activity of metabolite of effective microorganism preparation can adjust animal body according to the study
Function, promote animal body produce antibody, improve animal immunizing power, at the same time, many beneficial microbes inherently have suppression
Bacterium act on, in into animal body after, the growth to pathogenic microorganism is inhibited.
Occur in that substantial amounts of employing microorganism formulation is added in animal feed as additive in prior art, so as to reality
Now strengthen the disease resistance of animal body, the purpose of enhance immunity, such as number of patent application are a kind of 201310465699.4 treatment
The Chinese medicine microecological preparation of bovine subclinical mastitis, it adopts probiotic bacteria and Chinese medicine composition etc. to carry out preparing Tiny ecosystem system
Agent so that it can improve milk cow immunity, realizes the treatment to cow recessive mastitis；For another example number of patent application is
201610343971.5 animal microbial ecological agent and its application, its active component is with fermentation medium culture composite bacteria agent capable
The culture for obtaining；The active component of the composite bacteria agent capable is made up of Bacillus subtillis and aspergillus niger, is fattened with reaching promotion
The growth performance of pig, significantly improves the total augment weight and daily gain of pig, significantly reduces feedstuff-meat ratio, saves feed cost；In addition,
Also chicken growth performance, chicken antioxygenic property and chicken immune performance can be improved.
Even so, in prior art for microbial ecological agent in animal feed as additive, or in animal body
Application in terms of immunity improvement, there is substantial amounts of research, but, for the continuous exploration of microbial ecological agent, it is still ability
The direction that field technique personnel are continually striving to, and in prior art, for the research of the enhanced microbial ecological agent of calf immunity
It is also relatively fewer.
The content of the invention
To realize object above, the technical scheme is that and a kind of microbial ecological agent for improving calf immunity is provided,
It is made up of Bafillus natt liquid, Bacillus licheniformis liquid, Lactobacillus plantarum liquid, beer yeast bacterium solution, its bacterium solution volume ratio is：
0.8-1.5：1-1.5：0.6-1.4：0.6-1.3.
It is preferred that, the microbial ecological agent contains carrier, and the carrier is one or more in mealy potato, Semen sojae atricolor powder.
It is preferred that, in the mixed bacteria liquid, Bacillus nattoSawamura bacterium solution, Bacillus licheniformis liquid, Lactobacillus plantarum bacterium solution,
The volume ratio of cereuisiae fermentum bacterium solution is 1：1.2：1：0.8.
It is preferred that, the mixed bacteria liquid is 1 with the envelope-bulk to weight ratio of carrier：1.5.
A kind of preparation method of the microbial ecological agent for improving calf immunity, does not have pathogenic using itself, can produce many
Plant antibiotics and enzyme, the Bafillus natt with broad spectrum antibiotic activity and extremely strong anti-adversity ability, Bacillus licheniformis, plant
Lactobacilluss, cereuisiae fermentum carry out preferred amplification culture, and production technology adopts separation and Culture, addition to be suitable to thalli growth preservation
Carrier, its preparation method is：
The first step, breeding bacterial strain screening culture：
It is bacterium source to take food in the cud of nursing period ox rotten, and using MRS lactic acid bacteria culturing mediums lactic acid bacteria separation is carried out, and is screened
Go out Lactobacillus plantarum and Bacillus licheniformis；Cereuisiae fermentum is separated in beer fermentation liquid with yeast Selective agar medium；Big
Separating bacillus natto using nutrient agar in bean fermentation carries out detached strain streak culture, selects that growth is fast, bacterium
The obvious Bafillus natt of body characteristicses, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum；
Second step, breeding bacterial strain amplification culture：
Fast Bafillus natt will be grown in step 1 and will access the 100ml conical flasks equipped with 40ml seed culture mediums, 33
DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add in the 100ml conical flasks equipped with 40ml fermentation medium,
38 DEG C, 185r/min cultivate 24 hours, described fermentation medium components are glucose 1%, maltose 1%, soy peptone
8%, tryptone 4%, K2HPO40.1%, KH2PO40.6%, CaCl20.02%, MgSO40.05%, pH modulation 7；Culture
Zymocyte liquid dilution afterwards, bacterial concentration is more than 109Cell/ml, is placed in 4 DEG C of preservations.
By well-grown Bacillus licheniformis strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
By well-grown Lactobacillus plantarum strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
The preparation of cereuisiae fermentum bacterium solution：By the beer yeast strain that growth selection in step 1 is excellent, it is inoculated with into being equipped with
The 100ml conical flasks of 40mlYPD fluid mediums, 38 DEG C, culture 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml
In the 100ml conical flasks of ferment culture medium, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than
109Cell/ml, is placed in 4 DEG C of preservations.
3rd step, by the bacillus natto diluent of above-mentioned preparation, Bacillus licheniformis diluent, Lactobacillus plantarum dilution
Liquid, cereuisiae fermentum diluent press volume 0.8-1.5：1-1.5：0.6-1.4：0.6-1.3 makes mixed bacteria liquid.
4th step, is 1 by bacterium solution and carrier bulk and weight ratio：1.5 (v/w), the mixed bacteria liquid that step 3 is produced is added
Mix in ripening carrier.
The microbial ecological agent addition of made raising calf immunity is account for calf feed quality 0.1～3%.
The digestive system of calf itself is simultaneously unsound, and by nutrient substance enough in digestion feedstuff is decomposed environment is resisted
Unfavorable factor is difficult, and by adding bacillus natto, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum make for the invention
Calf it is edible after, calf intestinal micro-system can be improved, promote calf alimentation, improve calf resistance, promote calf
Intestinal digests, and strengthens calf immunity, reduces calf sickness rate, improves the calf speed of growth, reduces feedstuff-meat ratio.
Compared with prior art, the invention has following more excellent technique effect：
1st, adaptable breeding flora is taken using local environment, it is ensured that the high-speed rapid growth of strain and efficiently dividing for material
Solution, reduces the inadaptability to calf.
2nd, the regulation of Bafillus natt, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum to calf intestinal flora
Calf can be caused to adapt to more feedstuffs, expand the source of nutrient substance.
3rd, the immunity and environment resistance for improving calf is adopted this method, drug residue is not produced, is beef Gao Pin
The preferably guarantee of matter.
4th, the strain carrier cost for adopting is cheap.
Specific embodiment
Technical scheme is further limited with reference to specific embodiment, but claimed
Scope is not only limited to description.
In the examples below, for the embodiment of microbial ecological agent product is：
The microbial ecological agent of calf immunity is improved, by Bafillus natt liquid, Bacillus licheniformis liquid, Lactobacillus plantarum
Liquid, beer yeast bacterium solution are made, and its bacterium solution volume ratio is：0.8-1.5：1-1.5：0.6-1.4：0.6-1.3.
In certain embodiments, the microbial ecological agent contains carrier, and the carrier is the one kind in mealy potato, Semen sojae atricolor powder
Or it is several.
In certain embodiments, in the mixed bacteria liquid, Bacillus nattoSawamura bacterium solution, Bacillus licheniformis liquid, plant
Lactobacilluss bacterium solution, the volume ratio of cereuisiae fermentum bacterium solution are 1：1.2：1：0.8.
In certain embodiments, the mixed bacteria liquid and the envelope-bulk to weight ratio of carrier are 1：1.5.
Specific product can be prepared according to the preparation method for implementing to operate in detail below：
Embodiment 1
A kind of preparation method of the microorganism formulation for improving calf immunity, its step is as follows
The first step, breeding bacterial strain screening culture：
It is bacterium source to take food in the cud of nursing period ox rotten, and using MRS lactic acid bacteria culturing mediums lactic acid bacteria separation is carried out, and is screened
Go out Lactobacillus plantarum and Bacillus licheniformis；Cereuisiae fermentum is separated in beer fermentation liquid with yeast Selective agar medium；Big
Separating bacillus natto using nutrient agar in bean fermentation carries out detached strain streak culture, selects that growth is fast, bacterium
The obvious Bafillus natt of body characteristicses, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum；
Second step, breeding bacterial strain amplification culture：
Fast Bafillus natt will be grown in step 1 and will access the 100ml conical flasks equipped with 40ml seed culture mediums, 33
DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add in the 100ml conical flasks equipped with 40ml fermentation medium,
38 DEG C, 185r/min cultivate 24 hours, described fermentation medium components are glucose 1%, maltose 1%, soy peptone
8%, tryptone 4%, K2HPO40.1%, KH2PO40.6%, CaCl20.02%, MgSO40.05%, pH modulation 7；Culture
Zymocyte liquid dilution afterwards, bacterial concentration is more than 109Cell/ml, is placed in 4 DEG C of preservations.
By well-grown Bacillus licheniformis strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
By well-grown Lactobacillus plantarum strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
The preparation of cereuisiae fermentum bacterium solution：By the beer yeast strain that growth selection in step 1 is excellent, it is inoculated with into being equipped with
The 100ml conical flasks of 40mlYPD fluid mediums, 38 DEG C, culture 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml
In the 100ml conical flasks of ferment culture medium, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than
109Cell/ml, is placed in 4 DEG C of preservations.
3rd step, the Bafillus natt diluent of above-mentioned preparation, Bacillus licheniformis diluent, Lactobacillus plantarum is dilute
It is by volume 1 to release liquid, cereuisiae fermentum diluent：1.2：1：0.8 makes mixed bacteria liquid.
4th step, carrier makes, 100 DEG C of high-temperature maturing 40min of Semen sojae atricolor powder, is 1 by bacterium solution and carrier bulk and weight ratio：
1.5 (v/w), the mixed bacteria liquid that step 3 is produced are added in cure soypowder and are mixed.
Experimental example 2
A kind of preparation method of the microorganism formulation for improving calf immunity, its step is as follows
The first step, breeding bacterial strain screening culture：
It is bacterium source to take food in the cud of nursing period ox rotten, and using MRS lactic acid bacteria culturing mediums lactic acid bacteria separation is carried out, and is screened
Go out Lactobacillus plantarum and Bacillus licheniformis；Cereuisiae fermentum is separated in beer fermentation liquid with yeast Selective agar medium；Big
Separating bacillus natto using nutrient agar in bean fermentation carries out detached strain streak culture, selects that growth is fast, bacterium
The obvious Bafillus natt of body characteristicses, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum；
Second step, breeding bacterial strain amplification culture：
Fast Bafillus natt will be grown in step 1 and will access the 100ml conical flasks equipped with 40ml seed culture mediums, 33
DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add in the 100ml conical flasks equipped with 40ml fermentation medium,
38 DEG C, 185r/min cultivate 24 hours, described fermentation medium components are glucose 1%, maltose 1%, soy peptone
8%, tryptone 4%, K2HPO40.1%, KH2PO40.6%, CaCl20.02%, MgSO40.05%, pH modulation 7；Culture
Zymocyte liquid dilution afterwards, bacterial concentration is more than 109Cell/ml, is placed in 4 DEG C of preservations.
By well-grown Bacillus licheniformis strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
By well-grown Lactobacillus plantarum strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
The preparation of cereuisiae fermentum bacterium solution：By the beer yeast strain that growth selection in step 1 is excellent, it is inoculated with into being equipped with
The 100ml conical flasks of 40mlYPD fluid mediums, 38 DEG C, culture 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml
In the 100ml conical flasks of ferment culture medium, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than
109Cell/ml, is placed in 4 DEG C of preservations.
3rd step, the Bafillus natt diluent of above-mentioned preparation, Bacillus licheniformis diluent, Lactobacillus plantarum is dilute
It is by volume 1 to release liquid, cereuisiae fermentum diluent：1.2：1：0.8 makes mixed bacteria liquid.
4th step, carrier makes, 100 DEG C of ripening 40min of mealy potato, is 1 by bacterium solution and mealy potato volume and weight ratio：
1.5 (v/w), the mixed bacteria liquid that step 3 is produced are added in ripening mealy potato and are mixed.
Experimental example 3
A kind of preparation method of the microorganism formulation for improving calf immunity, its step is as follows
The first step, breeding bacterial strain screening culture：
It is bacterium source to take food in the cud of nursing period ox rotten, and using MRS lactic acid bacteria culturing mediums lactic acid bacteria separation is carried out, and is screened
Go out Lactobacillus plantarum and Bacillus licheniformis；Cereuisiae fermentum is separated in beer fermentation liquid with yeast Selective agar medium；Big
Separating bacillus natto using nutrient agar in bean fermentation carries out detached strain streak culture, selects that growth is fast, bacterium
The obvious Bafillus natt of body characteristicses, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum；
Second step, breeding bacterial strain amplification culture：
Fast Bafillus natt will be grown in step 1 and will access the 100ml conical flasks equipped with 40ml seed culture mediums, 33
DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add in the 100ml conical flasks equipped with 40ml fermentation medium,
38 DEG C, 185r/min cultivate 24 hours, described fermentation medium components are glucose 1%, maltose 1%, soy peptone
8%, tryptone 4%, K2HPO40.1%, KH2PO40.6%, CaCl20.02%, MgSO40.05%, pH modulation 7；Culture
Zymocyte liquid dilution afterwards, bacterial concentration is more than 109Cell/ml, is placed in 4 DEG C of preservations.
By well-grown Bacillus licheniformis strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
By well-grown Lactobacillus plantarum strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
The preparation of cereuisiae fermentum bacterium solution：By the beer yeast strain that growth selection in step 1 is excellent, it is inoculated with into being equipped with
The 100ml conical flasks of 40mlYPD fluid mediums, 38 DEG C, culture 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml
In the 100ml conical flasks of ferment culture medium, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than
109Cell/ml, is placed in 4 DEG C of preservations.
3rd step, the Bafillus natt diluent of above-mentioned preparation, Bacillus licheniformis diluent, Lactobacillus plantarum is dilute
It is by volume 1 to release liquid, cereuisiae fermentum diluent：1.2：1：0.8 makes mixed bacteria liquid.
4th step, carrier makes, and is by weight 1 by Semen sojae atricolor powder and mealy potato：1 mixing, 100 DEG C of ripening 40min, by bacterium
Liquid is 1 with carrier bulk and weight ratio：1.5 (v/w), by the mixed bacteria liquid that step 3 is produced cure soypowder and Rhizoma Solani tuber osi are added to
Mix in powder.
Experimental example 4
A kind of preparation method of the microorganism formulation for improving calf immunity, its step is as follows
The first step, breeding bacterial strain screening culture：
It is bacterium source to take food in the cud of nursing period ox rotten, and using MRS lactic acid bacteria culturing mediums lactic acid bacteria separation is carried out, and is screened
Go out Lactobacillus plantarum and Bacillus licheniformis；Cereuisiae fermentum is separated in beer fermentation liquid with yeast Selective agar medium；Big
Separating bacillus natto using nutrient agar in bean fermentation carries out detached strain streak culture, selects that growth is fast, bacterium
The obvious Bafillus natt of body characteristicses, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum；
Second step, breeding bacterial strain amplification culture：
Fast Bafillus natt will be grown in step 1 and will access the 100ml conical flasks equipped with 40ml seed culture mediums, 33
DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add in the 100ml conical flasks equipped with 40ml fermentation medium,
38 DEG C, 185r/min cultivate 24 hours, described fermentation medium components are glucose 1%, maltose 1%, soy peptone
8%, tryptone 4%, K2HPO40.1%, KH2PO40.6%, CaCl20.02%, MgSO40.05%, pH modulation 7；Culture
Zymocyte liquid dilution afterwards, bacterial concentration is more than 109Cell/ml, is placed in 4 DEG C of preservations.
By well-grown Bacillus licheniformis strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
By well-grown Lactobacillus plantarum strain is taken in step 1, it is inoculated with into equipped with 40mlMRS fluid mediums
100ml conical flasks, 38 DEG C, 185r/min cultivate 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml fermentation medium
In 100ml conical flasks, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than 108Cell/ml, is placed in 4
DEG C preserve.
The preparation of cereuisiae fermentum bacterium solution：By the beer yeast strain that growth selection in step 1 is excellent, it is inoculated with into being equipped with
The 100ml conical flasks of 40mlYPD fluid mediums, 38 DEG C, culture 24 hours；Take 2% (v/v) seed liquor to add equipped with 40ml
In the 100ml conical flasks of ferment culture medium, 38 DEG C, culture 24 hours, be obtained zymocyte liquid, dilution bacterial concentration be more than
109Cell/ml, is placed in 4 DEG C of preservations.
3rd step, the Bafillus natt diluent of above-mentioned preparation, Bacillus licheniformis diluent, Lactobacillus plantarum is dilute
It is by volume 1 to release liquid, cereuisiae fermentum diluent：1.2：1：0.8 makes mixed bacteria liquid.
4th step, carrier makes, 100 DEG C of ripening 40min of Semen sojae atricolor powder, by Semen sojae atricolor powder and Fructus Ligustri Lucidi, Herba Epimedii by weight
For 3:1:1 is mixed and made into carrier, is 1 by bacterium solution and carrier bulk and weight ratio：1.5 (v/w), the mixing that step 3 is produced
Bacterium solution is added in carrier powder and mixes.
For the invention, in research process, following test has been done：
Test example 1：
Test calf provides the calf 290 of close 60 kilograms of body weight for Guangxi plant, and every calf injects 0.5ml
Culture fluid containing influenza virus and diarrhea viruses, routine feeding two weeks has 280 calf morbidities.7 groups are randomly divided into, experiment
60 days, per group routinely feedstuff fed, its process is as follows：
A groups are using traditional Western medicine injection treatment；
B groups adopt Bacillus nattoSawamura fermentation liquid, the lichen bacillus ferments liquid, Lactobacillus plantarum fermentation liquid, medicated beer ferment
Female fermented liquid proportioning is 1：1.2：1：0.8 microbial ecological agent of the present invention, allocates in feedstuff for 0.1% according to quality accounting and feeds
Cowboying calf；
C groups adopt Bacillus nattoSawamura fermentation liquid, the lichen bacillus ferments liquid, Lactobacillus plantarum fermentation liquid, medicated beer ferment
Female fermented liquid proportioning is 0.9：1：0.8：0.6 microbial ecological agent of the present invention, allocates in feedstuff for 2% according to quality accounting and feeds
Cowboying calf；
D groups adopt Bacillus nattoSawamura fermentation liquid, the lichen bacillus ferments liquid, Lactobacillus plantarum fermentation liquid, medicated beer ferment
Female fermented liquid proportioning is 1.2：1.3：0.1：1 microbial ecological agent of the present invention, allocates in feedstuff for 1% according to quality accounting and feeds
Cowboying calf；
E groups are added in calf feed with microbial ecological agent using the animal of Application No. 201610343971.5 and feed cattle
Calf.
Each group strange land, while test, it is to avoid affect each other.
Observation each group test, observes calf Cure, counts body weight, feedstuff scale of feeding, the material of each group calf
, to cure number/sum, feedstuff-meat ratio is feedstuff scale of feeding/body weight evolution for meat ratio, cure rate, effective percentage, wherein medicine effective percentage,
Result of the test result is as shown in the table：
Result of the test shows that microbial ecological agent of the present invention effectively increases calf feed intake, improves feed digestibility, drop
Low feedstuff-meat ratio, hence it is evident that improve calf resistance, the effective percentage of healing is substantially better than micro- in market medicament and contrast patent
Ecological reagent.
Test example 2
Test calf is the calf 60 at the monthly age of Guangxi plant one, is randomly divided into tri- groups of a, b, c.Measure respectively initial
Body is high, body is long, and telophasic body Gao Tichang, 60 days experimental periods are measured after off-test.
A groups：Feeding microbial ecological agent of the present invention.Wherein, Bacillus nattoSawamura bacterium solution, Bacillus licheniformis liquid, plant
Lactobacilluss bacterium solution, cereuisiae fermentum bacterium solution proportioning are 1.2：1.3：0.1：1, carrier is that Semen sojae atricolor powder presses weight with Fructus Ligustri Lucidi, Herba Epimedii
Amount is than being 3:1:1 is mixed.Microbial ecological agent is by 2% addition feedstuff of feeding convention amount.
B groups：Feeding microbial ecological agent of the present invention.Wherein, Bacillus nattoSawamura bacterium solution, Bacillus licheniformis liquid, plant
Lactobacilluss bacterium solution, cereuisiae fermentum bacterium solution proportioning are 1.2：1.3：0.1：1, its carrier is Semen sojae atricolor powder.Microbial ecological agent is by feeding
In 2% addition feedstuff of convention amount.
C groups：Feeding conventional feed.
Experimental result
Result of the test shows, microbial ecological agent of the present invention, for the build bone growth of cattle all improves a lot.For
There is very big promotional value for the fattening of calf.
Here it is important to point out that, above example be only limitted to be further elaborated technical scheme and
Illustrate, be not the further restriction to technical scheme.
Claims (7)
1. it is a kind of improve calf immunity microbial ecological agent, it is characterised in that by Bacillus nattoSawamura fermentation liquid, lichens spore
Bacillus fermentation liquid, Lactobacillus plantarum fermentation liquid, beer yeast fermented liquid are by volume 0.8-1.5：1-1.5：0.6-1.4：
0.6-1.3 makes mixed bacteria liquid.
2. it is according to claim 1 improve calf immunity microbial ecological agent, it is characterised in that the microbial ecological agent
Containing carrier, the carrier is one or more in mealy potato, Semen sojae atricolor powder.
3. it is according to claim 1 improve calf immunity microbial ecological agent, it is characterised in that the mixed bacteria liquid
In, Bacillus nattoSawamura bacterium solution, Bacillus licheniformis liquid, Lactobacillus plantarum bacterium solution, the volume ratio of cereuisiae fermentum bacterium solution are
1：1.2：1：0.8.
4. it is according to claim 2 improve calf immunity microbial ecological agent, it is characterised in that the mixed bacteria liquid with
The envelope-bulk to weight ratio of carrier is 1：1.5.
5. according to power any one of 1-4 described in raising calf immunity microbial ecological agent preparation method, it is characterised in that system
Standby step is as follows：
The first step, breeding bacterial strain screening culture：Take food in the cud of nursing period ox it is rotten for bacterium source, filter out Lactobacillus plantarum and
Bacillus licheniformis；Cereuisiae fermentum is separated in beer fermentation liquid with yeast Selective agar medium；Isolate in fermented soybean
Bacillus natto, detached strain is carried out it is streak culture, pick out growth fast, the obvious Bafillus natt of thalline feature,
Clothing bacillus cereuss, Lactobacillus plantarum, cereuisiae fermentum；
Second step, breeding bacterial strain amplification culture：
The excellent Bafillus natt of character, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum will be picked out in step 1,
It is respectively configured seed culture medium and is enlarged culture；After the completion of culture, Bafillus natt bacterium solution be diluted to concentration >=
109The liquid of cell/ml, Bacillus licheniformis liquid concentration >=10 are diluted to8The liquid of cell/ml, Lactobacillus plantarum bacterium
Liquid is diluted to concentration >=109The liquid of cell/ml, cereuisiae fermentum bacterium solution concentration >=10 are diluted to9The liquid of cell/ml；
3rd step, by the bacillus natto of above-mentioned preparation, Bacillus licheniformis, Lactobacillus plantarum, cereuisiae fermentum diluent body is pressed
Product is than being 0.8-1.5：1-1.5：0.6-1.4：0.6-1.3 makes mixed bacteria liquid；
4th step, carrier makes, and takes 100 DEG C of ripening carrier 40min of carrier mass high temperature, by bacterium solution and carrier bulk and weight ratio
For 1：1.5 (v/w), the mixed bacteria liquid that step 3 is produced are added in ripening carrier and are mixed.
6. the microbial ecological agent for improving calf immunity or as claimed in claim 5 as described in any one of claim 1-4
Microbial ecological agent prepared by the preparation method of the microbial ecological agent of raising calf immunity is in calf feed as additive.
7. microbial ecological agent as claimed in claim 6 in calf feed as additive, it is characterised in that addition is to account for
The 0.1～3% of calf feed quality.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101700105A (en) * | 2009-11-24 | 2010-05-05 | 黄建壮 | Microecological feed additive for milk cows and preparation method thereof |
| CN102077904A (en) * | 2010-03-11 | 2011-06-01 | 哈尔滨龙猪科技开发有限公司 | Method for preparing biological feed additive of lactobacillus |
| CN103385360A (en) * | 2013-08-14 | 2013-11-13 | 双胞胎（集团）股份有限公司 | Simple and efficient fermented soybean meal production technology and application thereof |
| CN105483050A (en) * | 2015-12-31 | 2016-04-13 | 北京英惠尔生物技术有限公司 | Lactobacillus plantarum strain and application thereof in fermented feed |
-
2016
- 2016-11-25 CN CN201611055798.5A patent/CN106578450A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101700105A (en) * | 2009-11-24 | 2010-05-05 | 黄建壮 | Microecological feed additive for milk cows and preparation method thereof |
| CN102077904A (en) * | 2010-03-11 | 2011-06-01 | 哈尔滨龙猪科技开发有限公司 | Method for preparing biological feed additive of lactobacillus |
| CN103385360A (en) * | 2013-08-14 | 2013-11-13 | 双胞胎（集团）股份有限公司 | Simple and efficient fermented soybean meal production technology and application thereof |
| CN105483050A (en) * | 2015-12-31 | 2016-04-13 | 北京英惠尔生物技术有限公司 | Lactobacillus plantarum strain and application thereof in fermented feed |
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