CN105519823A - Special fly maggot immune feed for aquatic products and preparation method thereof - Google Patents
Special fly maggot immune feed for aquatic products and preparation method thereof Download PDFInfo
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- CN105519823A CN105519823A CN201610040247.5A CN201610040247A CN105519823A CN 105519823 A CN105519823 A CN 105519823A CN 201610040247 A CN201610040247 A CN 201610040247A CN 105519823 A CN105519823 A CN 105519823A
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Abstract
The invention discloses special fly maggot immune feed for aquatic products. The special fly maggot immune feed is prepared from the following ingredients in parts by weight: 50 to 60 parts of fly maggot bioactive peptide, 20 to 30 parts of Chinese herbal medicine, 0.8 to 1.6 parts of bifidobacterium and 0.6 to 2.8 parts of lactobacilli. The invention also discloses a preparation method of the special fly maggot immune feed for aquatic products. The method comprises the following steps of 1, fly maggot bioactive peptide preparation: grinding fly maggots to prepare grinding liquid; centrifugally collecting supernate; coarsely filtering the supernate by a filter membrane for use; 2, Chinese herbal medicine preparation: slicing radix astragali and liquorice, and crushing the sliced radix astragali and liquorice together with medicated leaven, cordyceps militaris and hawthorn; 3, fermentation for feed production: inoculating strain liquid of the lactobacilli, and firstly performing anaerobic fermentation for 48 hours at a fermentation temperature being 25 to 35 DEG C; then, inoculating the strain liquid of the bifidobacterium, and performing anaerobic fermentation for 48 to 72 hours; preparing fermentation products. The prepared feed is safe and has no pollution, and the antibacterial capability of aquatic animals can be greatly improved.
Description
Technical field
The present invention relates to the technical field of aquatic feeds, particularly relate to special fly maggot immunization feedstuff of a kind of aquatic products and preparation method thereof.
Background technology
Housefly is Musca animal, is complete metamorphosis, comprises ovum, larva (fly maggot), pupa, adult (fly) four rank its history of life.Fly maggot is omnipresent at occurring in nature, possesses complete antibacterial, reproduction and damage repair mechanism, has extremely strong adaptive capacity to environment.This environmental adaptation mechanism is that housefly obtains in long-term evolutionary process.Research shows, containing powerful defense mechanism in fly maggot body, as the EGF etc. of the peroxidase of antibacterial peptide, oxidation resistance and metallothionein etc., self-repairing capability.These functional components of fly maggot are that disease medical applications provides valuable drug resource.
In culture fishery, be applied to a large amount of feeds, grow fast for making aquatic livestock, at present, in commercially available aquatic feeds, be mostly unfavorable for the composition of health of people containing hormone etc., and the antibacterial ability of these feeds to aquatic livestock is limited, correspondingly, the aquatic livestock of causing a disease does not have the object of weightening finish on the contrary.
In view of this, the present inventor studies and devises special fly maggot immunization feedstuff of a kind of aquatic products and preparation method thereof, and this case produces thus.
Summary of the invention
The object of the present invention is to provide the special fly maggot immunization feedstuff of a kind of aquatic products, to provide a kind of safe, pollution-free and the feed of aquatic livestock antibacterial ability can be improved.
Another object of the present invention is to the preparation method that the special fly maggot immunization feedstuff of a kind of aquatic products is provided, by the batch fermentation of two kinds of probiotic bifidobacterias and Bacillus acidi lactici, with the obtained special fly maggot immunization feedstuff of above-mentioned aquatic products.
To achieve these goals, the present invention solves the technical scheme that its technical problem takes and is:
The special fly maggot immunization feedstuff of a kind of aquatic products, comprises the raw material of following parts by weight: fly maggot active peptide 50-60 part, Chinese herbal medicine 20-30 part, Bifidobacterium 0.8-1.6 part and Bacillus acidi lactici 0.6-2.8 part.
As the preferred embodiment of embodiment, described Chinese herbal medicine is made up of the raw material of following parts by weight: Radix Astragali 8-12 part, Radix Glycyrrhizae 10-16 part, Divine Comedy 6-12 part, Cordceps militaris 4-8 part and hawthorn 5-7 part.
As the preferred embodiment of embodiment, described fly maggot active peptide 55 parts, 25 parts, described Chinese herbal medicine, described Bifidobacterium 1.2 parts and described Bacillus acidi lactici 1.8 parts.
As the preferred embodiment of embodiment, the described Radix Astragali 10 parts, 13 parts, described Radix Glycyrrhizae, described Divine Comedy 9 parts, described Cordceps militaris 6 parts and described hawthorn 6 parts.
As the preferred embodiment of embodiment, also comprise B B-complex, described B B-complex is made up of by weight 5:1:3 vitamin A, vitamine D3 and vitamin E.
A preparation method for the special fly maggot immunization feedstuff of aquatic products, comprises the following steps:
Step one, prepare fly maggot active peptide:
Ground by fly maggot, make lapping liquid, regulate lapping liquid pH to 7.8-8.5, with the centrifugal 25-30min of the rotating speed of 13000rpm, centrifuging temperature is 4 DEG C, collects supernatant, the ultrafiltration through membranes dammed with 60,000 Dalton molecular weights, collects permeate; Filter membrane coarse filtration is for subsequent use;
Step 2, prepare Chinese herbal medicine:
After the Radix Astragali, Radix Glycyrrhizae section, together with Divine Comedy, Cordceps militaris and hawthorn, join in pulverizer and pulverize, obtained granularity is 200-300 object Chinese herbal medicine;
Step 3, fermentation production of fodder:
By fly maggot active peptide and Chinese herbal medicine 121 DEG C of sterilizing 20-30min, be cooled to room temperature, add water, stir, obtained solid fermentation culture medium, the water content of solid fermentation culture medium is 5-10%, the strain liquid of access Bacillus acidi lactici, first anaerobic fermentation 48h, fermentation temperature 25-35 DEG C; Access the strain liquid of Bifidobacterium again, then anaerobic fermentation 48-72h, fermentation temperature 25-45 DEG C; Obtained tunning; Tunning is dried, bake out temperature 55-75 DEG C, the obtained special fly maggot immunization feedstuff of described aquatic products.
As the preferred embodiment of embodiment, the preparation of the strain liquid of described Bacillus acidi lactici comprises the rejuvenation of Bacillus acidi lactici and expands cultivation, the culture medium that described rejuvenation adopts comprises casein peptone 10g/L, glucose 15g/L, dipotassium hydrogen phosphate 2g/L and magnesium sulfate 0.5g/L, and all the other components are water; Described rejuvenation culture medium pH6-7, rejuvenation temperature is 25-35 DEG C, rejuvenation time 24h.
As the preferred embodiment of embodiment, the preparation of the strain liquid of described Bifidobacterium comprises bacterial classification recovery and expands cultivates, the preparation method of the culture medium that described bacterial classification recovery adopts is: add in distilled water by 200-300g corn flour, stir evenly, slow fire boils rear timing 1h, filtered through gauze, after adding 15-20g agar, heating is dissolved, and supplies the water yield to 1000mL; Packing, 121 DEG C of sterilizing 20min.
The present invention with fly maggot, Chinese herbal medicine for raw material, obtained by the batch fermentation of two kinds of probiotic bifidobacterias and Bacillus acidi lactici, technique is simple, obtained aquatic products special fly maggot immunization feedstuff is safe and effective, nutritious, substantially increase the antibacterial ability of aquatic livestock, fully can meet the needs of aquaculture.
Detailed description of the invention
Embodiment 1
The special fly maggot immunization feedstuff of a kind of aquatic products, comprises the raw material of following parts by weight: fly maggot active peptide 50 parts, 20 parts, Chinese herbal medicine, Bifidobacterium 0.8 part and Bacillus acidi lactici 0.6 part.
As the preferred embodiment of embodiment 1, described Chinese herbal medicine is made up of the raw material of following parts by weight: the Radix Astragali 8 parts, 10 parts, Radix Glycyrrhizae, Divine Comedy 6 parts, Cordceps militaris 4 parts and hawthorn 5 parts.
As the preferred embodiment of embodiment 1, also comprise B B-complex, described B B-complex is made up of by weight 5:1:3 vitamin A, vitamine D3 and vitamin E.
Embodiment 2
The special fly maggot immunization feedstuff of a kind of aquatic products, comprises the raw material of following parts by weight:, described fly maggot active peptide 55 parts, 25 parts, described Chinese herbal medicine, described Bifidobacterium 1.2 parts and described Bacillus acidi lactici 1.8 parts.
As the preferred embodiment of embodiment 2, the described Radix Astragali 10 parts, 13 parts, described Radix Glycyrrhizae, described Divine Comedy 9 parts, described Cordceps militaris 6 parts and described hawthorn 6 parts.
As the preferred embodiment of embodiment 2, also comprise B B-complex, described B B-complex is made up of by weight 5:1:3 vitamin A, vitamine D3 and vitamin E.
Embodiment 3
The special fly maggot immunization feedstuff of a kind of aquatic products, comprises the raw material of following parts by weight: fly maggot active peptide 60 parts, 30 parts, Chinese herbal medicine, Bifidobacterium 1.6 parts and Bacillus acidi lactici 2.8 parts.
As the preferred embodiment of embodiment, described Chinese herbal medicine is made up of the raw material of following parts by weight: the Radix Astragali 12 parts, 16 parts, Radix Glycyrrhizae, Divine Comedy 12 parts, Cordceps militaris 8 parts and hawthorn 7 parts.
As the preferred embodiment of embodiment, also comprise B B-complex, described B B-complex is made up of by weight 5:1:3 vitamin A, vitamine D3 and vitamin E.
Embodiment 4
A preparation method for the special fly maggot immunization feedstuff of aquatic products, comprises the following steps:
Step one, prepare fly maggot active peptide:
Ground by fly maggot, make lapping liquid, regulate lapping liquid pH to 7.8-8.5, with the centrifugal 25-30min of the rotating speed of 13000rpm, centrifuging temperature is 4 DEG C, and collect supernatant, filter membrane coarse filtration is for subsequent use;
Step 2, prepare Chinese herbal medicine:
After the Radix Astragali, Radix Glycyrrhizae section, together with Divine Comedy, Cordceps militaris and hawthorn, join in pulverizer and pulverize, obtained granularity is 200-300 object Chinese herbal medicine;
Step 3, fermentation production of fodder:
By fly maggot active peptide and Chinese herbal medicine 121 DEG C of sterilizing 20-30min, be cooled to room temperature, add water, stir, obtained solid fermentation culture medium, the water content of solid fermentation culture medium is 5-10%, the strain liquid of access Bacillus acidi lactici, first anaerobic fermentation 48h, fermentation temperature 25-35 DEG C; Access the strain liquid of Bifidobacterium again, then anaerobic fermentation 48-72h, fermentation temperature 25-45 DEG C; Obtained tunning; Tunning is dried, bake out temperature 55-75 DEG C, the obtained special fly maggot immunization feedstuff of described aquatic products.
As the preferred embodiment of embodiment 4, the preparation of the strain liquid of described Bacillus acidi lactici comprises the rejuvenation of Bacillus acidi lactici and expands cultivation, the culture medium that described rejuvenation adopts comprises casein peptone 10g/L, glucose 15g/L, dipotassium hydrogen phosphate 2g/L and magnesium sulfate 0.5g/L, and all the other components are water; Described rejuvenation culture medium pH6-7, rejuvenation temperature is 25-35 DEG C, rejuvenation time 24h.
As the preferred embodiment of embodiment 4, the preparation of the strain liquid of described Bifidobacterium comprises bacterial classification recovery and expands cultivates, the preparation method of the culture medium that described bacterial classification recovery adopts is: add in distilled water by 200-300g corn flour, stir evenly, slow fire boils rear timing 1h, filtered through gauze, after adding 15-20g agar, heating is dissolved, and supplies the water yield to 1000mL; Packing, 121 DEG C of sterilizing 20min.
Penaeus Vannmei is cultivated, adopt feed of the present invention compared with commercially available conventional feed, the former is used to improve 20-30% than using the Penaeus Vannmei speed of growth of the latter, save feed 10-15%, the incidence of disease reduces 90%, and the culture-cycle shortens 2-3 month, product head is large, counterpoise improves more than 15%, and safety, pollution-free.
All distortion that those of ordinary skill in the art can directly derive from the disclosure of invention or associate, all should think protection scope of the present invention.
Claims (8)
1. the special fly maggot immunization feedstuff of aquatic products, is characterized in that: the raw material comprising following parts by weight: fly maggot active peptide 50-60 part, Chinese herbal medicine 20-30 part, Bifidobacterium 0.8-1.6 part and Bacillus acidi lactici 0.6-2.8 part.
2. the special fly maggot immunization feedstuff of a kind of aquatic products as claimed in claim 1, is characterized in that: described Chinese herbal medicine is made up of the raw material of following parts by weight: Radix Astragali 8-12 part, Radix Glycyrrhizae 10-16 part, Divine Comedy 6-12 part, Cordceps militaris 4-8 part and hawthorn 5-7 part.
3. the special fly maggot immunization feedstuff of a kind of aquatic products as claimed in claim 1, is characterized in that: described fly maggot active peptide 55 parts, 25 parts, described Chinese herbal medicine, described Bifidobacterium 1.2 parts and described Bacillus acidi lactici 1.8 parts.
4. the special fly maggot immunization feedstuff of a kind of aquatic products as claimed in claim 2, is characterized in that: the described Radix Astragali 10 parts, 13 parts, described Radix Glycyrrhizae, described Divine Comedy 9 parts, described Cordceps militaris 6 parts and described hawthorn 6 parts.
5. the special fly maggot immunization feedstuff of a kind of aquatic products as claimed in claim 1, is characterized in that: also comprise B B-complex, and described B B-complex is made up of by weight 5:1:3 vitamin A, vitamine D3 and vitamin E.
6. a preparation method for the special fly maggot immunization feedstuff of aquatic products, is characterized in that: comprise the following steps:
Step one, prepare fly maggot active peptide:
Ground by fly maggot, make lapping liquid, regulate lapping liquid pH to 7.8-8.5, with the centrifugal 25-30min of the rotating speed of 13000rpm, centrifuging temperature is 4 DEG C, and collect supernatant, filter membrane coarse filtration is for subsequent use;
Step 2, prepare Chinese herbal medicine:
After the Radix Astragali, Radix Glycyrrhizae section, together with Divine Comedy, Cordceps militaris and hawthorn, join in pulverizer and pulverize, obtained granularity is 200-300 object Chinese herbal medicine;
Step 3, fermentation production of fodder:
By fly maggot active peptide and Chinese herbal medicine 121 DEG C of sterilizing 20-30min, be cooled to room temperature, add water, stir, obtained solid fermentation culture medium, the water content of solid fermentation culture medium is 5-10%, the strain liquid of access Bacillus acidi lactici, first anaerobic fermentation 48h, fermentation temperature 25-35 DEG C; Access the strain liquid of Bifidobacterium again, then anaerobic fermentation 48-72h, fermentation temperature 25-45 DEG C; Obtained tunning; Tunning is dried, bake out temperature 55-75 DEG C, the obtained special fly maggot immunization feedstuff of described aquatic products.
7. the preparation method of the special fly maggot immunization feedstuff of a kind of aquatic products as claimed in claim 6, it is characterized in that: the preparation of the strain liquid of described Bacillus acidi lactici comprises the rejuvenation of Bacillus acidi lactici and expands cultivation, the culture medium that described rejuvenation adopts comprises casein peptone 10g/L, glucose 15g/L, dipotassium hydrogen phosphate 2g/L and magnesium sulfate 0.5g/L, and all the other components are water; Described rejuvenation culture medium pH6-7, rejuvenation temperature is 25-35 DEG C, rejuvenation time 24h.
8. the preparation method of the special fly maggot immunization feedstuff of a kind of aquatic products as claimed in claim 6, it is characterized in that: the preparation of the strain liquid of described Bifidobacterium comprises bacterial classification recovery and expands cultivates, the preparation method of the culture medium that described bacterial classification recovery adopts is: add in distilled water by 200-300g corn flour, stir evenly, slow fire boils rear timing 1h, filtered through gauze, after adding 15-20g agar, heating is dissolved, and supplies the water yield to 1000mL; Packing, 121 DEG C of sterilizing 20min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113317414A (en) * | 2021-05-08 | 2021-08-31 | 深圳市博裕家园生物科技有限公司 | Fly maggot slurry and preparation method thereof |
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