CN107594090A - A kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff - Google Patents
A kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff Download PDFInfo
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- CN107594090A CN107594090A CN201711042156.6A CN201711042156A CN107594090A CN 107594090 A CN107594090 A CN 107594090A CN 201711042156 A CN201711042156 A CN 201711042156A CN 107594090 A CN107594090 A CN 107594090A
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- fly maggot
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention relates to a kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff, belong to protein feed preparing technical field, it comprises the following steps：(1) fly maggot worm is cultivated；(2) double fermentation；(3) prepared by protein feed.Insect microbial cell broken wall fermentation protein feedstuff provided by the present invention, in fly maggot worm after the feather meal fermented through feed fermentation agent is added into hatching, so that microorganism and fly maggot worm symbiosis in feather meal, thoroughly decomposed by the synergy feather meal of microorganism and fly maggot worm, so that the protein in feather meal is fully used, and it is prepared into the composite protein feed containing Multiple components such as fly-maggot protein, plant degradation protein concentrate, mycoprotein, polypeptide, amino acid, chitin and probiotics.
Description
Technical field
The invention belongs to insect protein feed preparing technical field, is broken in particular to a kind of insect microbial cell
The preparation method of wall fermentation protein feedstuff.
Background technology
Existing livestock and poultry cultivation feed is formed using former material material assemblies such as corn and soybean, wheat bran and rape cakes more, a side
The content of face, its albumen and fat is limited, in order to reach ideal culture efficiency, it is necessary to put into higher feed cost；
On the other hand, livestock and poultry are often subject to the puzzlement of germ in breeding process, gently then influence the normal development and growth of indivedual livestock and poultry,
It is heavy then cause pestilence to cause large batch of death, huge economic loss is brought to raiser；Many raisers add in feed
Add relative medicine to improve the immunity of livestock and poultry, the economic loss died of illness and brought is reduced, however, so can be residual inside livestock and poultry
Certain drug ingedient is stayed, directly affects animal meat quality, endangers the health of human body of consumer.
Meanwhile feather meal resource is extremely abundant, crude protein content is also very high, is the hat of animal protein feed, and exploitation is sharp
With the keratoprotein that absorption is not readily dissolved in feather meal, have become an important research and development to alleviate China's protein feeds deficiency
Approach.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of feeding of insect microbial cell broken wall fermentation protein
The preparation method of material, so that the protein in feather meal is fully used, and it is prepared into dense containing fly-maggot protein, plant degradation
The composite protein feed of the Multiple components such as pix protein, mycoprotein, polypeptide, amino acid, chitin and probiotics.
The technical solution adopted in the present invention is：
A kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff, it comprises the following steps：
(1) fly maggot worm is cultivated：First, it is uniformly mixed, prepares with probiotics bacterial liquid after wheat bran and peanut cake being crushed
Into fly-maggot feed；Then, fly maggot ovum is added in fly-maggot feed and carries out hatching culture, and added after fly maggot egg hatching adult flies maggot
Enter the feather meal fermented through feed fermentation agent to continue to cultivate；Wherein, caused protease juice in fly maggot worm growth course
Keratoprotein in feather meal is thoroughly decomposed, the feather meal after decomposition can preferably digest and assimilate it during animal feeding
Middle protein；
(2) double fermentation：After fly maggot worm in the step (1) is developed to the fly maggot worm of maturation, become using fly maggot worm
The principle of dark lucifuge separates the fly maggot worm of maturation with analyte；Wherein, the ripe fly maggot worm being partially separated out is successively through disappearing
Crushed after poison, rinsing processing and prepare adult slurry；The ripe fly maggot worm being partially separated out is pupated emergence, and the pupa after emergence is passed through
Crushed after sterilization, rinsing processing and prepare pupa slurry；Strain is inoculated with respectively in the analyte that worm slurry, pupa are starched and isolated to be sent out
Ferment, the strain include bacillus subtilis, lactobacillus acidophilus and saccharomyces cerevisiae；
(3) worm slurry, pupa slurry and analyte one after being fermented in the step (2) are dried under conditions of arising from 50-60 DEG C,
It is prepared into the composite protein feed containing Multiple components.
Preferably, in the step (1), the temperature of fly maggot ovum culture is 27-30 DEG C, and the relative humidity of fly maggot ovum culture is
65%-70%.
Preferably, in the step (1), the temperature of fly maggot ovum culture is 30 DEG C, and the relative humidity of fly maggot ovum culture is
70%.
Preferably, in the step (1), add after hatching culture 24 hours in fly maggot ovum addition fly-maggot feed and sent out through feed
The feather meal that ferment agent ferments；In the step (2), the fly maggot ovum in step (1) adds hatching culture 4 days in fly-maggot feed
Fly maggot worm is separated with analyte afterwards.
Preferably, the step (1), wheat bran, peanut cake, the weight ratio of probiotics bacterial liquid are 1：1：2.
Preferably, in the step (1), the weight ratio of fly-maggot feed and fly maggot ovum is 100：1.
Preferably, in the step (1), the feather meal and the weight ratio of fly maggot ovum that are fermented through feed fermentation agent are 25：
1。
Preferably, in the step (3), by the worm slurry in the step (2) and analyte respectively in 50-60 DEG C of condition
It is lower fermentation 72 hours after, one arise from 50-60 DEG C under conditions of dry.
Preferably, in the step (2), the inoculation of bacillus subtilis, lactobacillus acidophilus and saccharomyces cerevisiae in strain
The ratio of amount is 1：1：1.
Preferably, in the step (1), wheat bran and groundnut meal is broken to after 100 mesh and are stirred with probiotics bacterial liquid
Uniformly.
Beneficial effects of the present invention：
Insect microbial cell broken wall fermentation protein feedstuff provided by the present invention, the plumage that will be fermented through feed fermentation agent
Hair powder is added in the fly maggot worm after hatching so that microorganism and fly maggot worm symbiosis in feather meal, passes through microorganism and fly maggot worm
Synergy feather meal is thoroughly decomposed so that the protein in feather meal is fully used, and be prepared into containing
The Multiple components such as fly-maggot protein, plant degradation protein concentrate, mycoprotein, polypeptide, amino acid, chitin and probiotics it is compound
Protein feed.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical scheme will be carried out below
Detailed description.Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are resulting on the premise of creative work is not made to be owned
Other embodiment, belong to the scope that the present invention is protected.
Embodiment 1
A kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff is present embodiments provided, it includes following
Specific steps：
(1) fly maggot worm is cultivated：First, it is uniformly mixed, prepares with probiotics bacterial liquid after wheat bran and peanut cake being crushed
Into fly-maggot feed；Then, fly maggot ovum is added in fly-maggot feed and carries out hatching culture, and added after fly maggot egg hatching adult flies maggot
Enter the feather meal fermented through feed fermentation agent to continue to cultivate；Wherein, caused protease juice in fly maggot worm growth course
Keratoprotein in feather meal is thoroughly decomposed, the feather meal after decomposition can preferably digest and assimilate it during animal feeding
Middle protein；
(2) double fermentation：After fly maggot worm in the step (1) is developed to the fly maggot worm of maturation, become using fly maggot worm
The principle of dark lucifuge separates the fly maggot worm of maturation with analyte；Wherein, wherein, the ripe fly maggot worm being partially separated out is successively
Crushed after sterilized, rinsing processing and prepare adult slurry；The ripe fly maggot worm being partially separated out pupates emergence, and by after emergence
Pupa is sterilized, preparation pupa slurry is crushed after rinsing processing；Strain is inoculated with respectively in the analyte that worm slurry, pupa are starched and isolated to enter
Row fermentation, the strain include bacillus subtilis, lactobacillus acidophilus and saccharomyces cerevisiae；
(3) prepared by protein feed：Worm slurry, pupa slurry and analyte one after being fermented in the step (2) arise from 50-60 DEG C
Under conditions of dry, be prepared into containing fly-maggot protein, plant degradation protein concentrate, mycoprotein, polypeptide, antibacterial peptide, amino acid,
The composite protein feed of the Multiple components such as chitin and probiotics.
In the present embodiment, the weight ratio of wheat bran, peanut cake and probiotics bacterial liquid in fly-maggot feed is preferably 1：1：2, from
And be advantageous to the feed growth of the fly maggot worm after hatching；Also, the weight ratio preferably 100 of fly-maggot feed and fly maggot ovum：1, from
And can avoid influenceing the healthy growth of fly maggot worm because fly-maggot feed deficiency or stocking rate are excessive, be advantageous to improve fly maggot worm
Quality.Meanwhile the weight ratio preferably 25 of feather meal and fly maggot ovum：1：.
In the present embodiment, in the step (1), the temperature of fly maggot worm culture is preferably 27-30 DEG C, and more excellent is 30 DEG C；Fly
The relative humidity of maggot culture is preferably 65%-70%, and more excellent is 70%；Taken so as to which fly maggot egg hatching can be shortened
Between, it can ensure that fly maggot ovum has highest incubation rate again, and suitable growing environment is improved for the fly maggot worm of 1-2 ages,
Be advantageous to its decomposition to feather meal.
Wherein, when environment temperature is 30 DEG C, the brooding time of fly maggot ovum is 12 hours；When envionmental humidity is 70%,
It can think that the fly maggot worm of 1-2 ages improves suitable growing environment, the ripe fly maggot worm for being advantageous to for 3 ages again develops and changed
Pupa.
In the present embodiment, in step (2), the inoculation of bacillus subtilis, lactobacillus acidophilus and saccharomyces cerevisiae in strain
Amount is preferably 1：1：1.
Embodiment 2
A kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff is present embodiments provided, it includes following
Specific steps：
(1) fly maggot worm is cultivated：First, 100 jin of wheat bran and 100 jin of groundnut meals are broken to after 100 mesh and probiotics bacterial liquid
It is uniformly mixed, is prepared into fly-maggot feed；Then, it is 30 DEG C 4 jin of fly maggot ovum to be added in fly-maggot feeds with temperature, relatively wet
Spend to carry out hatching culture under conditions of 70%；100 jin are added after fly maggot egg hatching culture 1 day to ferment through feed fermentation agent
Feather meal continue to cultivate；Wherein, in fly maggot worm growth course caused protease juice by the keratoprotein in feather meal
Thoroughly decompose, the feather meal after decomposition can preferably digest and assimilate wherein protein during animal feeding；
(2) double fermentation：After hatching is cultivated 4 days in fly maggot ovum addition fly-maggot feed in the step (1), utilize
Fly maggot worm become dark lucifuge principle by maturation fly maggot worm separated with analyte；Wherein, the ripe fly maggot worm being partially separated out
Crushed after sterilized successively, rinsing processing and prepare adult slurry, make its breaking-wall cell；The ripe fly maggot worm being partially separated out pupates
Sprout wings, and by the pupa after emergence is sterilized, is crushed after rinsing processing and prepares pupa slurry；In worm slurry, pupa slurry and the decomposition isolated
Strain is inoculated with thing respectively to be fermented, the strain includes bacillus subtilis, lactobacillus acidophilus and saccharomyces cerevisiae, and withered grass
The ratio of the inoculum concentration of bacillus, lactobacillus acidophilus and saccharomyces cerevisiae is 1：1：1；
(3) prepared by protein feed：By the worm slurry in the step (2), pupa slurry and analyte respectively in 50-60 DEG C of bar
After being fermented 72 hours under part, one arise from 50-60 DEG C under conditions of dry, be prepared into containing fly-maggot protein, plant degradation concentrate egg
In vain, the composite protein feed of the Multiple components such as mycoprotein, polypeptide, antibacterial peptide, amino acid, chitin and probiotics.
The preparation method for the insect microbial cell broken wall fermentation protein feedstuff that above-described embodiment is provided, will send out through feed
The feather meal that ferment agent ferments is added in the fly maggot worm after hatching so that microorganism and fly maggot worm symbiosis in feather meal, is passed through
The synergy of microorganism and fly maggot worm causes feather meal thoroughly to decompose, so that the protein in feather meal obtains abundant profit
With, and be prepared into containing fly-maggot protein, plant degradation protein concentrate, mycoprotein, polypeptide, amino acid, chitin and probiotics
Deng the composite protein feed of Multiple components.
The composite protein feed prepared by above-mentioned preparation method not only its can substitute the high-quality animal egg such as fish meal completely
White protein sources, be a kind of high-quality, high safety, cost it is low, it is renewable, can industrialization animal-based protein feed；Moreover,
Also there is the advantages of nutritious, balanced, protein content is high, and amino acid is comprehensive, balanced, and absorption rate is high.
Wherein, the antibacterial peptide being rich in composite protein feed has innate immunity effect to animal, and there is broad-spectrum sterilization to live
Property, there is efficient lethal effect to Gram-positive and gramnegative bacterium, reported at least more than 130 kinds both at home and abroad
Different bacterium can be killed by antibacterial peptide；Meanwhile antibacterial peptide has obvious lethal effect to some fungi, protozoon and virus.
A large amount of amino acid, mycoprotein, peptide material and the probiotics contained in composite protein feed can significantly increase
Animal immunizing power, premunition, improve aquatic environment, reduce disease, improve production performance.
The chitin contained in composite protein feed can improve the immunity function of body, strengthen the increasing of animal immune cell
Grow, therefore have the effect of reinforced immunological power.
It should be noted that probiotics bacterial liquid and feed fermentation agent respectively by existing EM strains it is fermented after prepare and
Into it can be commercially available on the market.
In above-described embodiment, probiotics bacterial liquid and feed fermentation agent are purchased from Microbiology Research Inst., Guangzhou City；Wherein, it is prebiotic
Effective total viable count >=1.0 × 10 of bacterium bacterium solution9CFU/mL, pH 3.5-5.5.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff, it is characterised in that comprise the following steps：
(1) fly maggot worm is cultivated：First, it is uniformly mixed after wheat bran and peanut cake being crushed with probiotics bacterial liquid, prepares adult flies
Maggot feed；Then, fly maggot ovum is added in fly-maggot feed and carries out hatching culture, and warp is added after fly maggot egg hatching adult flies maggot
The feather meal that feed fermentation agent ferments continues to cultivate；Wherein, in fly maggot worm growth course caused protease juice by plumage
Mao Fenzhong keratoprotein thoroughly decomposes, and the feather meal after decomposition can preferably be digested and assimilated therein during animal feeding
Protein；
(2) double fermentation：After fly maggot worm in the step (1) is developed to the fly maggot worm of maturation, is become using fly maggot worm and secretly kept away
The principle of light separates the fly maggot worm of maturation with analyte；Wherein, the ripe fly maggot worm that is partially separated out is sterilized successively, drift
Preparation adult slurry is crushed after washing processing；The ripe fly maggot worm being partially separated out pupates emergence, and by the pupa after emergence it is sterilized,
Crushed after rinsing processing and prepare pupa slurry；Strain is inoculated with respectively in the analyte that worm slurry, pupa are starched and isolated to be fermented, institute
Stating strain includes bacillus subtilis, lactobacillus acidophilus and saccharomyces cerevisiae；
(3) prepared by protein feed：Worm slurry, pupa slurry and analyte one after being fermented in the step (2) arise from 50-60 DEG C of bar
Dried under part, be prepared into the composite protein feed containing Multiple components.
2. the preparation method of insect microbial cell broken wall fermentation protein feedstuff according to claim 1, it is characterised in that
In the step (1), the temperature of fly maggot ovum culture is 27-30 DEG C, and the relative humidity of fly maggot ovum culture is 65%-70%.
3. the preparation method of insect microbial cell broken wall fermentation protein feedstuff according to claim 2, it is characterised in that
In the step (1), the temperature of fly maggot ovum culture is 30 DEG C, and the relative humidity of fly maggot ovum culture is 70%.
4. the preparation method of insect microbial cell broken wall fermentation protein feedstuff according to claim 3, it is characterised in that
In the step (1), fly maggot ovum adds hatching culture in fly-maggot feed and adds the plumage fermented through feed fermentation agent after 12 hours
Mao Fen；In the step (2), by fly maggot worm with dividing after hatching is cultivated 4 days in the fly maggot ovum addition fly-maggot feed in step (1)
Solve thing separation.
5. the preparation method of the insect microbial cell broken wall fermentation protein feedstuff according to claim any one of 1-4, its
It is characterised by, the step (1), wheat bran, peanut cake, the weight ratio of probiotics bacterial liquid are 1：1：2.
6. the preparation method of insect microbial cell broken wall fermentation protein feedstuff according to claim 5, it is characterised in that
In the step (1), the weight ratio of fly-maggot feed and fly maggot ovum is 100：1.
7. the preparation method of insect microbial cell broken wall fermentation protein feedstuff according to claim 6, it is characterised in that
In the step (1), the feather meal and the weight ratio of fly maggot ovum that are fermented through feed fermentation agent are 25：1.
8. the preparation method of insect microbial cell broken wall fermentation protein feedstuff according to claim 7, it is characterised in that
In the step (3), the worm slurry in the step (2) is fermented 72 under conditions of 50-60 DEG C respectively with pupa slurry and analyte
After individual hour, one arise from 50-60 DEG C under conditions of dry.
9. the preparation method of insect microbial cell broken wall fermentation protein feedstuff according to claim 8, it is characterised in that
In the step (2), the ratio of the inoculum concentration of bacillus subtilis, lactobacillus acidophilus and saccharomyces cerevisiae in strain is 1：1：1.
10. the preparation method of insect microbial cell broken wall fermentation protein feedstuff according to claim 9, its feature exist
In in the steps (1), wheat bran and groundnut meal being broken to after 100 mesh and are uniformly mixed with probiotics bacterial liquid.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371238A (en) * | 2018-02-10 | 2018-08-07 | 汪逸凡 | A kind of feed takes off mould dose of preparation method |
| CN113317414A (en) * | 2021-05-08 | 2021-08-31 | 深圳市博裕家园生物科技有限公司 | Fly maggot slurry and preparation method thereof |
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Patent Citations (6)
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| WO2005036981A1 (en) * | 2003-10-21 | 2005-04-28 | University Of Guelph | Method for enriching nervonic acid in expressed milk of ruminants |
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| Publication number | Priority date | Publication date | Assignee | Title |
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Application publication date: 20180119 |