CN102100302A - Preparation method of fermentation cake and maggot protein feed - Google Patents
Preparation method of fermentation cake and maggot protein feed Download PDFInfo
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- CN102100302A CN102100302A CN2010105900395A CN201010590039A CN102100302A CN 102100302 A CN102100302 A CN 102100302A CN 2010105900395 A CN2010105900395 A CN 2010105900395A CN 201010590039 A CN201010590039 A CN 201010590039A CN 102100302 A CN102100302 A CN 102100302A
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Abstract
The invention discloses a preparation method of a fermentation cake and maggot protein feed, and the method comprises the following steps: 1) smashing subsidiary agricultural product cakes serving as raw materials; 2) adding bran and bacillus subtilis into the smashed raw materials and uniformly mixing; 3) adding water in the product obtained in the step 2) and concocting to obtain a fermentation medium; 4) fermenting the fermentation medium and engineering fly eggs in a fermentation reactor; and 5) separating the product through fermentation in the step 4) and extracting the maggot larvae to obtain a ferment; and sequentially and respectively drying and smashing the maggot larvae and the ferment to obtain maggot protein and fermentation protein feed respectively. The maggot protein and fermentation protein feed prepared by the method have the characteristics of high protein content, nutritive equilibrium and the like.
Description
Technical field
The present invention relates to a kind of method of utilizing microorganism solid fermentation and fly maggot that inferior agricultural byproducts conversion technology is produced high-quality forage protein.
Background technology
Along with the development of China's feed industry, the shortage of protein feeds raw material has become first restriction factor of feed industrial development.
China is oil crops plantation big country in the world, the output of rape, peanut, cottonseed, sesame all occupies the No. 1 in the world, can produce a large amount of grouts in the process of annual production edible oil, but because the process technology degree is not high, the not high and most of grouts of the grouts quality of producing contain a large amount of ANFs, so that the grouts utilization rate is not high.
The gross protein value of dregs of beans is very high, is the base stock of preparation livestock and poultry feed, and is rich in several amino acids, and its protein biological transformation ratio is lower when still directly feeding livestock and poultry with dregs of beans, mainly is owing to contain many ANFs in the dregs of beans.
Rapeseed dregs is one of the abundantest plant-derived albumen of China, and its protein content is 28%～44%, the amino acid Compositional balance.But noxious materials such as the sulphur glucoside, tannin and the cellulose that exist in the rapeseed dregs and other ANFs have had a strong impact on dish dregs of rice feeding value.
Cotton cake dregs is rich in protein, is good plant protein fodder resource.But cotton cake dregs contains the noxious material gossypol and can produce, grow animal and aspect such as breeding produces tangible harmful effect; Be rich in ANFs such as material that cellulose, hemicellulose etc. are difficult to digest and phytic acid; Protein quality is poor, and cottonseed protein and rate of ultilization of amino acid are low, amino acid imbalance.Above-mentioned effects limit cotton cake dregs safety and effectively as animal protein feed, the serious waste of feed resource is caused in some areas even it is demoted make fertilizer.
Other grouts are same owing to process or the ANFs that exists own, and feeding value seriously is restricted.
Microorganism and fly maggot bio-transformation agricultural byproducts are produced high-quality protein feed and can be brought very big comprehensive benefit and have vast potential for future development.
Summary of the invention
The technical problem to be solved in the present invention provide a kind of easy and simple to handle, processing cost is low and product protein content height, the fermentation grouts of nutrient balance and the preparation method of fly-maggot protein fodder.
In order to solve the problems of the technologies described above, the invention provides a kind of preparation method of ferment grouts and fly-maggot protein fodder, may further comprise the steps:
1), be raw material with the agricultural byproducts grouts, with raw material pulverizing to can mistake 40～60 mesh sieves;
2), after the pulverizing of step 1) gained, add wheat bran in the raw material and mix, compound, wheat bran is 25%～75% with the weight ratio of pulverizing the back raw material; Add bacillus subtilis and mix in compound, the weight ratio of bacillus subtilis and compound is 1 ‰～5%;
3), in step 2) add water in the product of gained and modulate, fermentation medium; Moisture in the described fermentation medium is 45%～75%;
4), fermentation medium is packed in the fermentation reactor, then the engineering fly blow is spread in uniform in the fermented and cultured primary surface, even shakedown spills the fermentation medium that one deck can cover the engineering fly blow on the engineering fly blow again, ferment at last, total consumption of fermentation medium with the ratio of engineering fly blow is: the every kilogram of total consumption of fermentation medium/10000～50000 engineering fly blows; Fermentation temperature is 30 ℃～45 ℃, and fermentation time is 84～96 hours;
5), the product of step 4) fermentation back gained is separated, isolate the fly maggot larva after, material must ferment; Fly maggot larva and fermentation material branch are else carried out drying and pulverizing successively, get fly-maggot protein and fermentation protein feedstuff respectively.
Above-mentioned steps 5) isolated fly maggot larva is the worm that lives.
The drying of fly maggot larva was: in 60～140 ℃ of dryings 0.5～5 hour.The drying of fermentation material was: in 100～150 ℃ of dryings 0.5～3 hour.
Improvement as the preparation method of fermentation grouts of the present invention and fly-maggot protein fodder: the agricultural byproducts grouts are dregs of beans, the dish dregs of rice, cotton dregs, peanut meal or sesame seed meal.
Further improvement as the preparation method of fermentation grouts of the present invention and fly-maggot protein fodder: living bacteria count 100～50,000,000,000 of bacillus subtilis/gram.
Further improvement as the preparation method of fermentation grouts of the present invention and fly-maggot protein fodder: the engineering fly blow is the worm's ovum that engineering fly sexal maturity post-coitum is produced, and the feeding environment of engineering fly is: 25 ℃～35 ℃ of temperature, humidity (relative humidity) 50%～70%.Used engineering fly blow is a live body in the step 4).After the engineering fly blow output, generally about 12 hours, will hatch into larva naturally; Therefore, the engineering fly blow should promptly come into operation after the output, must come into operation before hatching into larva at the latest.
Further improvement as the preparation method of fermentation grouts of the present invention and fly-maggot protein fodder: in the step 4): the thickness of the fermentation medium that spills on engineering fly blow upper berth is 3～7mm, be generally (that is, consumption gets final product not allow the engineering fly blow be exposed in the air) about 5mm.
Further improvement as the preparation method of fermentation grouts of the present invention and fly-maggot protein fodder: in the sweat of step 4), when fermentation temperature 〉=35 ℃, carried out the one-time mechanical stirring every 10～14 hours.
In the present invention, step 1) is sieved raw material pulverizing, and purpose is in order to remove impurity such as wood chip in the grouts, aggregate chips, irony; Used water should select the cleaning water source that does not have contaminated mistake and do not contain foreign material in the step 3); Step 4) is carried out mechanical stirring during the fermentation in good time.
In the present invention, engineering fly and bacillus subtilis all can obtain by commercial form.
The present invention utilizes microorganism that the lower grouts of quality are fermented, and removes the ANFs in the grouts, thereby improves efficiency of feed utilization and feeding quality.Grouts in microbial fermentation, utilize engineering fly maggot as bioreactor to microorganism in the grouts can't utilize, decomposition but composition that engineering fly maggot can utilize, decompose carries out bio-transformation, thereby produce high-quality protein feed.
The quality of fermentation protein feedstuff of the present invention and fly-maggot protein is as shown in table 1 below:
Table 1,
| Fermentation protein feedstuff (%) | Fly-maggot protein (%) | |
| Dry | 91.2 | 90.0 |
| Crude protein | 25～55 | 60～65 |
| Crude fat | 2.7～6.5 | 10.3～12.5 |
| Coarse ash | 6.0～11.0 | 7.2～8.8 |
The method that microorganism of the present invention and fly maggot bio-transformation agricultural byproducts are produced high-quality protein feed is different from traditional solid state fermentation, traditional solid state fermentation just uses a microorganism of microbiological treatment solid material to decompose and synthetic simple procedure, production process has several factors inconvenience control, and product quality is extremely unstable.And in the present invention, grouts in microbial fermentation, utilize engineering fly maggot as bioreactor to microorganism in the grouts can't utilize, decomposition but composition that engineering fly maggot can utilize, decompose carries out bio-transformation, produce high-quality protein feed.In microbial fermentation and engineering maggot grow process, physics, the chemical environment that both produce can reduce single microorganism fermentation originally and the required external world of engineering fly maggot breeding helps, and has also reduced cost when raising the efficiency greatly.Production operation is simple, be easy to control, and product quality is guaranteed relatively.Product contains abundant antibacterial peptide class, significantly reduces traditional antibiotic use; Main nutrition nutrient meets or exceeds fish meal.
The fly maggot feeding habits are assorted, and the raising raw material sources are extensive, and agricultural byproducts leftover bits and pieces such as wheat bran, rice bran, vinasse, bean dregs can be used for fly maggot breeding.And the premunition of fly maggot is extremely strong, can grow in the environment of extreme difference.In the energy of the ecosystem transformed, assimilatory efficiency was mammiferous about half, but its production efficiency is mammiferous 15～40 times, was that to produce animal protein with additive method so far incomparable.Fly maggot is the animal protein feed of high-quality, the TEAA of former material of fly maggot and dry powder is respectively 44.09% and 43.83%, equal reference values 40% that proposes above Food and Agriculture Organization/World Health Organization (FAO/WHO), its TEAA/nonessential amino acid total amount value (E/N) is respectively 0.79,0.78, all the reference value 0.6 that proposes above FAO/WHO.
Method of the present invention has following beneficial effect:
(1) the present invention adopts microorganism solid fermentation technology, according to the characteristics of solid state fermentation and the characteristic of maggot grow microorganism solid fermentation and the raising of engineering fly maggot are combined, in the process of integrated treatment material, the two utilizes environmental physics, the electrochemical conditions of creating separately, the efficient of comparing the raising of originally single solid state fermentation and engineering fly maggot is higher;
The external environmental condition that need manually keep when (2) originally single solid state fermentation and engineering fly maggot being raised relies on mutual effect just can reach naturally, reduces operational sequence and difficulty greatly, reduces production costs.And can produce two kinds of high-quality protein feed-----fermentation protein feedstuffs and fly-maggot protein, therefore have very big comprehensive social benefit.
(3) production process is easy to control, and product quality is guaranteed relatively, and production efficiency is higher, and production cost reduces greatly.
Adopt the fermentation protein feedstuff of the inventive method gained, have following advantage:
1. improve crude protein.Because microbial fermentation " concentrating effect " and the generation of mycoprotein, grouts raw material protein content behind microbial fermentation improves greatly;
2. increase digestive utilization ratio.Through the decomposition of microorganism, the crude fibre of nondigestible decomposition in the grouts can be decomposed, reduce the ANFs in the material, improve the quality of grouts raw material greatly, improve the rate that digests and absorbs;
3. improve palatability.After material underwent microbial fermentation, material had the fragrance of fermentation.Thereby the increase palatability improves feed intake;
4. can reduce the quantity of the harmful bacterium of fowl poultry rear intestinal.Nonruminant can't utilize polysaccharide materials such as cellulose, and after these materials entered small intestine, its carbon source just acted on the culture medium of enteron aisle anaerobic bacteria, and harmful bacterium is bred in a large number, makes livestock and poultry produce the diarrhea phenomenon, influences livestock and poultry and grows normally.In the daily ration that contains the polysaccharide material, add the fermentation grouts, can make polysaccharide in the daily ration in the absorption that is decomposed of the leading portion of small intestine, thereby the nutrient source of the harmful bacterium of blocking-up intestines limit back segment, its quantity is subdued, thereby the water content that can reduce ight soil in addition again alleviates diarrhoea, the diarrhea of piglet, poult, produces and economic benefit thereby improve;
5. in feed, add the endogenous enzymes that the fermentation grouts can replenish nonruminant.Because grouts are through the speck fermentation, wherein contain a large amount of enzymes, add to and can make enzyme increase in the feed in the feed, can replenish the various enzymes of poult poultry secretory volume deficiency, not only can make and reach certain level in its body, and can also activate the secretion of original endogenous enzymes, be beneficial to poult poultry digesting and assimilating to feed.
6. in feed, add the fermentation grouts and can improve immunity and the metabolic hormone level of domestic animals and fowls.Can improve the immunity of poult poultry after a large amount of small-molecular peptides that cottonseed cake produces during the fermentation enters in the body, reduce the generation of disease.Various enzymes also can make metabolism hormones such as the more pancreas islet rope of fowl poultry secretion, thyroid-stimulating hormone, T3 simultaneously.
7. in feed, add the fermentation grouts and can reduce viscosity in the feed.Contain in the energy feed that more SNSP adds in preparation and to add a certain amount of fermentation grouts, owing to contain a large amount of enzyme preparations in the fermentation grouts, can make the polysaccharide in the feed be degraded to micromolecular polysaccharide fragment, thereby the chyme viscosity is reduced greatly, thereby improved the effect of endogenous enzymes, with the penetrating power of nutrient, digestibility and absorptivity are improved.
Adopt the inventive method gained fly-maggot protein, have the following advantages:
The nutrition of common house-fly grub is comprehensive, and the fly maggot larva contains 16 kinds of main amino acid, contain the required several amino acids of animal, and content mostly is higher than fish meal.Macroelement and micronutrient levels are also very abundant in the fly maggot, have detected more than 17 kinds.Not only macroelement potassium, sodium, calcium, phosphorus content are higher, and trace element zinc, magnesium, iron, copper, selenium, germanium, manganese content are also higher.The content of fat-soluble VA and VD is very abundant in the fly maggot worm powder, and especially the content of VD can be suitable with the content of VD in the fish liver.In the water soluble vitamin, B family vitamin content is abundanter.In addition, fly maggot also contains chitin, antibacterial peptide, antibacterial protein isoreactivity material.Contain abundant antibacterial peptide, can significantly reduce the use amount of antibiotics or not use antibiotic.
The fermentation protein feedstuff of gained of the present invention and the detailed directions of fly-maggot protein and consumption are as follows:
1), fermentation protein feedstuff:
After mixing according to certain proportioning with conventional feed, animal is fed according to the consumption and the usage of routine.That is, alternative original a fraction of conventional feed accounts for 5～25% of feed total amount to the alternative amount of conventional feed; The concrete amount that substitutes can be with reference to as follows:
Pig: sucking pig 10～25%, piggy 5～15%, in big pig 5～8%, farrowing sow 5～8%, milking sow 5～10%;
Fowl: egg fowl 10～20%, poultry 5-10;
Aquatic products: fresh-water fishes 5～15%, mandarin fish soft-shelled turtle seawater fish 10～20%, shrimp crab eel 10～25%.
Adding proportion can according to the different demands of raising object, raising stage and physiological status (lactation, anti-stress, growth promotion etc.), suitably adjust.
2), fly-maggot protein
After mixing according to certain proportioning with conventional feed, animal is fed according to the consumption and the usage of routine.That is, alternative original a fraction of conventional feed accounts for 1～20% of feed total amount to the alternative amount of conventional feed; The concrete amount that substitutes can be with reference to as follows:
Pig: newborn piggy 3～6%, in big pig 1～2%;
Fowl: egg fowl, young fowl 5～10%, poultry 3～5%;
Aquatic products: fresh-water fishes 5～20%.
Adding proportion can according to the different demands of raising object, raising stage and physiological status (lactation, anti-stress, growth promotion etc.), suitably adjust.
The specific embodiment
The used fermentation reactor of following examples is opened type.
The preparation method of embodiment 1, a kind of ferment grouts and fly-maggot protein fodder, carry out following steps successively:
1), be raw material with cotton dregs, utilize disintegrating apparatus to pulverize, cross 40 mesh sieves after the raw material pulverizing, purpose is to remove impurity such as wood chip in the cotton dregs, aggregate chips, irony; Must pulverize the back raw material;
2), after the pulverizing of step 1) gained, add in the raw material and account for the wheat bran of pulverizing back raw material weight 25% and mix, compound; Adding the bacillus subtilis that accounts for compound gross weight 5% in compound mixes; This bacillus subtilis is a pulvis, living bacteria count 10,000,000,000/gram;
3), in step 2) add the pure water modulation in the product of gained, moisture content be the fermentation medium of 45% (weight content); That is, amount of water is that to make the moisture in the fermentation medium be 45%; (general, as to record the moisture in the compound earlier, and then calculate the amount of water that needs, thereby guarantee that the moisture in the fermentation medium of final gained is 45%);
4), first preparation engineering fly blow, specific as follows:
(A) under 25 ℃～35 ℃, the environment of humidity 50%～70%, raise the engineering fly, treat to lay eggs after its sexal maturity;
A general engineering fly blow, the engineering fly blow of collection actual quantity needed gathered in 2 hours at interval when (B) sexal maturity of engineering fly is laid eggs.
The fermentation medium of step 3) gained is packed in the fermentation reactor, then the engineering fly blow is spread in uniform in the fermented and cultured primary surface, again on the engineering fly blow evenly shakedown spill the fermentation medium that one deck can cover the engineering fly blow (thickness be about 5mm, promptly, consumption gets final product not allow the engineering fly blow be exposed in the air), ferment at last; Total consumption of fermentation medium (i.e. the consumption sum of 2 place's fermentation mediums) with the ratio of engineering fly blow is: the every kilogram of total consumption of fermentation medium/10000 engineering fly blows; Fermentation temperature is 35 ℃～45 ℃, and fermentation time is 96 hours; During the fermentation, carried out the one-time mechanical stirring every 12 hours.
5), the product of step 4) fermentation back gained is separated, separation condition is specially: the negative phototaxis according to larva is separated with the method for illumination; After isolating the fly maggot larva, material must ferment; The fly maggot larva is carried out drying, and (baking temperature was dried 1～2 hour for about 100 ℃; ); Pulverize and cross 60 mesh sieves behind dry the end.The material that will ferment carries out drying, and temperature is 130 ℃, dry 1 hour; After finishing, drying pulverizes and crosses 60 mesh sieves.
The performance parameter of gained fly-maggot protein and fermentation protein feedstuff such as following table 2;
Table 2
| Fermentation protein feedstuff (%) | Fly-maggot protein (%) | |
| Dry | 91.2 | 90.0 |
| Crude protein | 45.7 | 63 |
| Crude fat | 3.7 | 11.8 |
| Coarse ash | 7.8 | 8.5 |
The preparation method of embodiment 2, a kind of ferment grouts and fly-maggot protein fodder, carry out following steps successively:
1), be raw material with the dish dregs of rice, utilize disintegrating apparatus to pulverize, cross 50 mesh sieves after the raw material pulverizing, purpose is to remove impurity such as wood chip in the dish dregs of rice, aggregate chips, irony; Must pulverize the back raw material;
2), after the pulverizing of step 1) gained, add in the raw material and account for the wheat bran of pulverizing back raw material weight 50% and mix, in compound, add the bacillus subtilis that accounts for compound gross weight 2.5% and mix; This bacillus subtilis is a pulvis, living bacteria count 30,000,000,000/gram;
3), in step 2) add pure water in the product of gained and modulate, moisture content be the fermentation medium of 60% (weight content);
4), the preparation method of engineering fly blow such as embodiment 1.
The fermentation medium of step 3) gained is packed in the fermentation reactor, then the engineering fly blow is spread in uniform in the fermented and cultured primary surface, again on the engineering fly blow evenly shakedown spill the fermentation medium that one deck can cover the engineering fly blow (thickness be about 5mm, promptly, consumption gets final product not allow the engineering fly blow be exposed in the air), ferment at last; Total consumption of fermentation medium (i.e. the consumption sum of 2 place's fermentation mediums) with the ratio of engineering fly blow is: the every kilogram of total consumption of fermentation medium/30000 engineering fly blows; Fermentation temperature is 35 ℃～45 ℃, and fermentation time is 96 hours; During the fermentation, carried out the one-time mechanical stirring every 12 hours.
5), the product of step 4) fermentation back gained is separated, separation condition is specially: the negative phototaxis according to larva is separated with the method for illumination; After isolating the fly maggot larva, material must ferment; The fly maggot larva is carried out drying (about 100 ℃ of baking temperatures, drying 1～2 hour), pulverize and cross 60 mesh sieves behind dry the end.The material that will ferment carries out drying, and temperature is 130 ℃, dry 1 hour; After finishing, drying pulverizes and crosses 60 mesh sieves.
The performance parameter of gained fly-maggot protein and fermentation protein feedstuff such as following table 3;
Table 3
| Fermentation protein feedstuff (%) | Fly-maggot protein (%) | |
| Dry | 91.2 | 90.0 |
| Crude protein | 43.6 | 60 |
| Crude fat | 3.4 | 10.3 |
| Coarse ash | 7.3 | 7.2 |
The preparation method of embodiment 3, a kind of ferment grouts and fly-maggot protein fodder, carry out following steps successively:
1), be raw material with the dregs of beans, utilize disintegrating apparatus to pulverize, cross 50 mesh sieves after the raw material pulverizing, purpose is to remove impurity such as wood chip in the dregs of beans, aggregate chips, irony; Must pulverize the back raw material;
2), after the pulverizing of step 1) gained, add in the raw material and account for the wheat bran of pulverizing back raw material weight 75% and mix, in compound, add the bacillus subtilis that accounts for compound gross weight 1 ‰ and mix; This bacillus subtilis is a pulvis, living bacteria count 50,000,000,000/gram;
3), in step 2) add pure water in the product of gained and modulate, moisture content be the fermentation medium of 75% (weight content);
4), the preparation method of engineering fly blow such as embodiment 1.
The fermentation medium of step 3) gained is packed in the fermentation reactor, then the engineering fly blow is spread in uniform in the fermented and cultured primary surface, again on the engineering fly blow evenly shakedown spill the fermentation medium that one deck can cover the engineering fly blow (thickness be about 5mm, promptly, consumption gets final product not allow the engineering fly blow be exposed in the air), ferment at last; Total consumption of fermentation medium (i.e. the consumption sum of 2 place's fermentation mediums) with the ratio of engineering fly blow is: the every kilogram of total consumption of fermentation medium/50000 engineering fly blows; Fermentation temperature is 35 ℃～45 ℃, and fermentation time is 96 hours; During the fermentation, carried out the one-time mechanical stirring every 12 hours.
5), the product of step 4) fermentation back gained is separated, separation condition is specially: the negative phototaxis according to larva is separated with the method for illumination; After isolating the fly maggot larva, material must ferment; The fly maggot larva is carried out drying successively and pulverizes and cross 60 mesh sieves.The material that will ferment carries out drying successively and pulverizes and cross 60 mesh sieves.
The performance parameter of gained fly-maggot protein and fermentation protein feedstuff such as following table 4;
Table 4
| Fermentation protein feedstuff (%) | Fly-maggot protein (%) | |
| Dry | 91.2 | 90.0 |
| Crude protein | 55 | 65 |
| Crude fat | 3.0 | 12.5 |
| Coarse ash | 7.0 | 8.8 |
In order to prove the purposes of gained fly-maggot protein of the present invention and fermentation protein feedstuff, the inventor has carried out following contrast experiment:
Test is divided into seven processed group, 4 repetitions of each processed group, and each repeats 12 pigs.Basal diet is according to NRC (1998) standard formulation, and deduction dregs of rice class raw material, and seven processed group are respectively:
First group: common cotton dregs group (the common cotton dregs of basal diet+10%; Promptly get) with alternative 10% basal diet of 10% common cotton dregs;
Second group: fermentation cotton dregs group (basal diet+10% the present invention cotton dregs that ferment; Promptly get) with alternative 10% basal diet of the fermentation protein feedstuff of 10% embodiment, 1 gained;
The 3rd group: common dish dregs of rice group (the common dish dregs of rice of basal diet+10%);
The 4th group: fermentation dish dregs of rice group (basal diet+10% the present invention dish dregs of rice that ferment, promptly substitute 10% basal diet and get) with the fermentation protein feedstuff of 10% embodiment, 2 gained;
The 5th group: common dregs of beans group (the common dregs of beans of basal diet+10%);
The 6th group: fermented bean dregs group (basal diet+10% a product fermented bean dregs promptly gets with alternative 10% basal diet of the fermentation protein feedstuff of 10% embodiment, 3 gained);
The 7th group: fly-maggot protein group (basal diet+10% a product fly-maggot protein promptly gets with alternative 10% basal diet of the fly-maggot protein of 10% embodiment, 1 gained).
Select the DLY tri-crossbreeding of (34 ± 1) age in days, raise 11d, the food consumption after finishing raised in record, calculates average weight gain, feedstuff-meat ratio and diarrhea rate in experimental period, result such as following table 5:
Table 5
Annotate: conventional protein groups is common dregs of beans group, that is, all feeds are formed by common dregs of beans.
As can be seen from Table 5, test group daily gain of the present invention all increases to some extent with respect to control group, and feedstuff-meat ratio all decreases than control group, and the diarrhea rate of test group of the present invention all take a favorable turn with respect to control group.The present invention compares with normal diet, has improved utilization rate, feed conversion rate, the enhancing immunity of organisms of product, has improved production performance.
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (6)
1. the preparation method of fermentation grouts and fly-maggot protein fodder is characterized in that may further comprise the steps:
1), be raw material with the agricultural byproducts grouts, with raw material pulverizing to can mistake 40～60 mesh sieves;
2), after the pulverizing of step 1) gained, add wheat bran in the raw material and mix, compound, described wheat bran is 25%～75% with the weight ratio of pulverizing the back raw material; Add bacillus subtilis and mix in compound, the weight ratio of described bacillus subtilis and compound is 1 ‰～5%;
3), in step 2) add water in the product of gained and modulate, fermentation medium; Moisture weight content in the described fermentation medium is 45%～75%;
4), described fermentation medium is packed in the fermentation reactor, then the engineering fly blow is spread in uniform in the fermented and cultured primary surface, even shakedown spills the fermentation medium that one deck can cover the engineering fly blow on the engineering fly blow again, ferment at last, total consumption of described fermentation medium with the ratio of engineering fly blow is: the every kilogram of total consumption of fermentation medium/10000～50000 engineering fly blows; Fermentation temperature is 30 ℃～45 ℃, and fermentation time is 84～96 hours;
5), the product of step 4) fermentation back gained is separated, isolate the fly maggot larva after, material must ferment; Fly maggot larva and fermentation material branch are else carried out drying and pulverizing successively, get fly-maggot protein and fermentation protein feedstuff respectively.
2. the preparation method of fermentation grouts according to claim 1 and fly-maggot protein fodder is characterized in that: described agricultural byproducts grouts are dregs of beans, the dish dregs of rice, cotton dregs, peanut meal or sesame seed meal.
3. the preparation method of fermentation grouts according to claim 2 and fly-maggot protein fodder is characterized in that: living bacteria count 100～50,000,000,000 of described bacillus subtilis/gram.
4. the preparation method of fermentation grouts according to claim 3 and fly-maggot protein fodder, it is characterized in that: described engineering fly blow is the worm's ovum that engineering fly sexal maturity post-coitum is produced, the feeding environment of described engineering fly is: 25 ℃～35 ℃ of temperature, humidity 50%～70%.
5. the preparation method of fermentation grouts according to claim 4 and fly-maggot protein fodder is characterized in that in the described step 4): the thickness of the fermentation medium that spills on engineering fly blow upper berth is 3～7mm.
6. the preparation method of fermentation grouts according to claim 5 and fly-maggot protein fodder is characterized in that: in the sweat of step 4), when fermentation temperature 〉=35 ℃, carried out the one-time mechanical stirring every 10～14 hours.
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| CN104222503A (en) * | 2014-09-04 | 2014-12-24 | 江门鑫肽生物蛋白有限公司 | Preparation method of novel insect fermented cottonseed meal protein |
| CN104886367A (en) * | 2015-05-15 | 2015-09-09 | 贵州梦润鹌鹑有限公司 | Immunity improving quail feed production method |
| CN106359850A (en) * | 2016-08-31 | 2017-02-01 | 郭舒洋 | Preparation method of high-protein fly maggot feed |
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| CN107484877A (en) * | 2017-07-27 | 2017-12-19 | 珠海天凯生物科技有限公司 | The preparation method of one Hylemyia Platura Meigen worm extract |
| CN107594090A (en) * | 2017-10-30 | 2018-01-19 | 广州昆虫蛋白生物科技有限公司 | A kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff |
| CN107691767A (en) * | 2017-10-26 | 2018-02-16 | 江门鑫肽生物蛋白有限公司 | A kind of scale fly maggot, probiotics symbiosis glycolysis technique |
| WO2018185474A1 (en) * | 2017-04-07 | 2018-10-11 | Entomics Biosystems Limited | Process for converting invertebrates into feedstock |
| CN108902468A (en) * | 2018-03-23 | 2018-11-30 | 新疆金胡杨牧业有限公司 | Maggot protein bioorganic feed and preparation method thereof |
| CN109874939A (en) * | 2019-04-23 | 2019-06-14 | 江门鑫肽生物蛋白有限公司 | A kind of antibacterial fly-maggot protein and preparation method thereof |
| CN111513183A (en) * | 2020-04-15 | 2020-08-11 | 广州无两生物科技有限公司 | Method for treating plant anti-nutritional factors by using insect larvae |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1242155A (en) * | 1998-07-17 | 2000-01-26 | 徐显和 | Feed contg. animal protein, and method for preparing same |
| CN101461443A (en) * | 2009-01-09 | 2009-06-24 | 邢志强 | Hydrolytic swelling feed and manufacturing method thereof |
-
2010
- 2010-12-07 CN CN201010590039A patent/CN102100302B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1242155A (en) * | 1998-07-17 | 2000-01-26 | 徐显和 | Feed contg. animal protein, and method for preparing same |
| CN101461443A (en) * | 2009-01-09 | 2009-06-24 | 邢志强 | Hydrolytic swelling feed and manufacturing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 《饲料博览》 20041231 李立 蛋白饲料专利技术概况 第35-37页 1-6 , 第10期 * |
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| CN103397068A (en) * | 2013-08-15 | 2013-11-20 | 河北大学 | Preparation method of fly maggot peptides |
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