CN111513183A - Method for treating plant anti-nutritional factors by using insect larvae - Google Patents
Method for treating plant anti-nutritional factors by using insect larvae Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a method for treating plant anti-nutritional factors by utilizing insect larvae, which comprises the steps of adding black soldier flies or larva eggs of yellow mealworms into feed, controlling the temperature of materials to be 28-35 ℃ in the breeding process of the black soldier flies or the yellow mealworms larvae, drying the black soldier flies and the materials at 80 ℃ after breeding time is 8-15 days, and detecting the content of each anti-nutritional factor. The invention can overcome the defects of removing the anti-nutritional factors in the prior art, such as consumption of original nutrient substances, chemical residue, high production cost, low removal rate of the anti-nutritional factors and the like. The invention combines three biological methods of enzyme preparation, compound strain and insect protein conversion, converts anti-nutritional factors and substances which are not beneficial to digestion and absorption of animals in vegetable protein raw materials into high-quality insect protein which is rich in probiotics and antibacterial substances and has reasonable amino acid composition.
Description
Technical Field
The invention belongs to the field of feed treatment, and particularly relates to a method for treating plant anti-nutritional factors by using insect larvae.
Background
Anti-nutritional factors are substances produced by plant metabolism that can destroy or prevent the digestive utilization of nutrients in the organism and adversely affect animal health and growth performance. It is a self-protective substance formed in the course of plant evolution, can protect plant strain and its seed from the invasion of mould, bacteria, virus, pest and birds, and can balance the nutrient substances in plant. Excessive consumption of these substances can adversely affect the absorption of animal nutrients and even cause poisoning. The passivation method of the anti-nutritional factors mainly comprises a physical method, a chemical method and a biological method.
The physical heating method is only suitable for heat-unstable antinutritional factors, and has poor effects on heat-stable antinutritional factors such as phytic acid, saponin, cyanogen compounds, oligosaccharides and the like. In the heating process, the anti-nutritional factors cannot be completely eliminated due to insufficient heating, and arginine, lysine and some sulfur-containing amino acids in the product are damaged due to excessive heating, so that the product is not desirable in production. Water infusion methods certain anti-nutritional factors are readily soluble in water and can be removed by this property. But the water is dried after soaking, the cost is higher, and the production is inconvenient.
The chemical method includes an acid-base treatment method, an ammonia treatment method, a treatment method of adding a specific substance, and the like. These methods can remove some of the anti-nutritional factors in the feed. Although the chemical method can save equipment and energy, the method has the defects of chemical substance residue, influence on feed palatability and environmental pollution.
The common vegetable protein raw materials in the feed at present comprise soybean meal, full-fat soybean, rapeseed meal, cottonseed meal, peanut meal and the like, and the raw materials are treated by multiple methods of puffing and chemical methods such as acid-base treatment, microbial fermentation and the like to eliminate anti-nutritional factors in the vegetable raw materials and other substances which are not beneficial to digestion and absorption of animals. However, the puffing process and the fermentation process both need to consume a large amount of energy and energy to achieve the aim, have the problems of high requirements on production equipment, complex process and the like, can also generate a large amount of waste water, waste gas and waste in the production process, and do not accord with the energy-saving and environment-friendly concept advocated by the state at present. The insect is utilized to treat the vegetable protein raw material, so that the purposes of eliminating anti-nutritional factors and substances which are not beneficial to digestion and absorption of animals can be achieved, another high-quality feeding protein is obtained after insect conversion, the special fragrant substances of the feeding protein can stimulate the feeding of animals, and various antibacterial substances contained in the feeding protein can improve the production performance of the animals and the flavor of meat products.
Disclosure of Invention
The present invention aims to overcome the above-mentioned disadvantages of the prior art and to provide a method for treating plant anti-nutritional factors using insect larvae. The invention combines three biological methods of enzyme preparation, compound strain and insect protein conversion, converts anti-nutritional factors and substances which are not beneficial to digestion and absorption of animals in vegetable protein raw materials into insect protein.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for processing plant anti-nutritional factors by using insect larvae comprises the steps of adding black soldier or larva eggs of yellow mealworms into feed, controlling the temperature of materials to be 28-35 ℃ in the breeding process of the black soldier fly or the yellow mealworm larvae, drying the black soldier fly larvae and the materials at 80 ℃ after breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
Further, the feed is processed by using the complex enzyme and the black water or the larva of the yellow meal worm in a combined way, and the method specifically comprises the following steps: treating the feed by using the complex enzyme, then adding larvae of the black soldier fly or the yellow mealworm, controlling the temperature of the material to be 28-35 ℃ in the breeding process of the black soldier fly or the yellow mealworm larvae, drying the black soldier fly larvae and the material at 80 ℃ after the breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
Further, the compound enzyme is phytase and cellulase, and the content of the compound enzyme is 100IU/g phytase and 200IU/g cellulase.
Further, the feed is processed by using the compound strains and the larvae of the black water or the yellow meal worms in a combined way, and specifically comprises the following steps: firstly, fermenting the composite strain to process feed, then adding larva eggs of the black soldier fly or the yellow mealworm, controlling the temperature of the material to be 28-35 ℃ in the breeding process of the black soldier fly or the yellow mealworm larvae, drying the black soldier fly larvae and the material at 80 ℃ after the breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
Further, the composite strain is saccharomyces boulardii, bacillus subtilis, lactobacillus plantarum, bacillus natto, beer yeast and lactobacillus casei; the mass ratio of each component is respectively 0.125 percent of Saccharomyces boulardii, 0.03 percent of Bacillus subtilis, 0.1 percent of Lactobacillus plantarum, 0.03 percent of Bacillus natto, 0.25 percent of beer yeast and 0.05 percent of Lactobacillus casei.
Further, the feed is jointly processed by using the complex enzyme, the complex strain and the black water or the larva of the yellow meal worm, and the method specifically comprises the following steps: firstly, pre-fermenting soybean meal by using a complex enzyme and a complex strain, then adding larvae eggs of black soldier or tenebrio molitor, controlling the material temperature at 28-35 ℃ in the breeding process of black soldier fly or tenebrio molitor larvae, drying the black soldier fly larvae and the material at 80 ℃ after the breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
Further, the feed is jointly processed by using the complex enzyme, the complex strain and the black water or the larva of the yellow meal worm, and the method specifically comprises the following steps: and (3) simultaneously adding the complex enzyme, the composite strain and the larvae eggs of the hermetia illucens or the tenebrio molitor into the feed, controlling the temperature of the materials to be 28-35 ℃ in the breeding process of the hermetia illucens or the tenebrio molitor larvae, drying the hermetia illucens and the materials at 80 ℃ after the breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
Further, the anti-nutritional factors include trypsin inhibitor, beta-conglycinin, urease and phytic acid.
Further, the feed comprises full-fat soybean, rapeseed meal, cottonseed meal and peanut meal.
The invention also provides a vegetable protein raw material prepared by the method.
The invention has the beneficial effects that: the invention can overcome the defects of removing the anti-nutritional factors in the prior art, such as consumption of original nutrient substances, chemical residue, high production cost, low removal rate of the anti-nutritional factors and the like. The invention combines three biological methods of enzyme preparation, compound strain and insect protein conversion, converts anti-nutritional factors and substances which are not beneficial to digestion and absorption of animals in vegetable protein raw materials into high-quality insect protein which is rich in probiotics and antibacterial substances and has reasonable amino acid composition.
Detailed Description
In order to more concisely and clearly demonstrate technical solutions, objects and advantages of the present invention, the present invention will be further described in detail with reference to specific embodiments.
Example 1
In the embodiment, the composite enzyme and the composite strain are adopted to pre-ferment the soybean meal, and then the black soldier fly larvae are added to treat the plant anti-nutritional factors, specifically as follows:
the compound enzyme comprises the following components: phytase is 100IU/g, cellulase is 200 IU/g; the composite strain comprises the following components: 0.125% of saccharomyces boulardii, 0.03% of bacillus subtilis, 0.1% of lactobacillus plantarum, 0.03% of bacillus natto, 0.25% of beer yeast and 0.05% of lactobacillus casei.
100kg of soybean meal, and adjusting the water content to 60%; and (3) adding the compound enzyme according to the proportion, simultaneously adding the compound strain into the soybean meal according to the inoculation amount of 0.59%, fermenting at 37 ℃ for 24h, fermenting at 30 ℃ for 24h, adding 10g of hermetia illucens eggs, controlling the material temperature at 28-35 ℃ in the breeding process of the hermetia illucens larvae, and drying the hermetia illucens larvae and the material at 80 ℃ after 10 days of breeding.
Example 2
In the embodiment, the anti-nutritional factors in the bean pulp are simultaneously processed by adopting the complex enzyme, the complex strain and the hermetia illucens larvae, and the method specifically comprises the following steps:
the compound enzyme comprises the following components: phytase is 100IU/g, cellulase is 200 IU/g; the composite strain comprises the following components: 0.125% of saccharomyces boulardii, 0.03% of bacillus subtilis, 0.1% of lactobacillus plantarum, 0.03% of bacillus natto, 0.25% of beer yeast and 0.05% of lactobacillus casei.
100kg of soybean meal, and adjusting the water content to 60%; simultaneously adding the complex enzyme, the compound strain with the inoculation amount of 0.59% and 10g of hermetia illucens eggs according to the proportion, and starting breeding hermetia illucens larvae: and keeping the temperature at 37 ℃ for the first 6h, controlling the temperature at 28-35 ℃ later, and drying the hermetia illucens larvae and the materials at 80 ℃ after 10 days of culture.
Example 3
In this example, only hermetia illucens larvae are used to treat anti-nutritional factors in soybean meal, which are as follows: 10g of hermetia illucens eggs are added into 100kg of soybean meal with the water content of 60%, the temperature of materials is controlled to be 28-35 ℃ in the breeding process of hermetia illucens larvae, and after the breeding time is 10 days, the hermetia illucens larvae and the materials are dried at 80 ℃.
Example 4
In this embodiment, the method of pretreating soybean meal with complex enzyme and then adding black soldier fly larvae is used for treating the plant anti-nutritional factors, and the method specifically comprises the following steps:
the compound enzyme comprises the following components: phytase is 100IU/g, cellulase is 200 IU/g;
the inoculation amount of the enzyme preparation: 0.59 percent;
100kg of soybean meal, and adjusting the water content to 60%; adding the complex enzyme into the soybean meal according to the proportion, carrying out enzyme treatment at 37 ℃ for 24 hours, and then carrying out enzyme treatment at 30 ℃ for 24 hours; then 10g of hermetia illucens eggs are added, the temperature of the materials is controlled to be 28-35 ℃ in the breeding process of the hermetia illucens larvae, and the hermetia illucens larvae and the materials are dried at 80 ℃ after 10 days of breeding.
Example 5
In this embodiment, the composite strains are used for pre-fermenting the soybean meal, and then the black soldier fly larvae are added to treat the plant anti-nutritional factors, specifically as follows:
the composite bacteria comprises the following components: 0.125% of saccharomyces boulardii, 0.03% of bacillus subtilis, 0.1% of lactobacillus plantarum, 0.03% of bacillus natto, 0.25% of beer yeast and 0.05% of lactobacillus casei;
100kg of soybean meal, and adjusting the water content to 60%; adding the composite strain into the soybean meal in an inoculation amount of 0.59%, fermenting at 37 ℃ for 24h, fermenting at 30 ℃ for 24h, adding 10g of hermetia illucens eggs, controlling the material temperature at 28-35 ℃ in the breeding process of the hermetia illucens larvae, breeding for 10 days, and drying the hermetia illucens larvae and the material at 80 ℃.
Example 6
The screened hermetia illucens dried in examples 1 to 5 were examined for trypsin inhibitor activity, β -conglycinin activity, urease activity, total pentosan content, soluble pentosan content, phytic acid content, and acid soluble protein content, and the results are shown in table 1. The trypsin inhibitor kit, the glycinin kit and the beta-conglycinin kit are purchased from Beijing Longke ark bioengineering technology, Inc.
1. Detection of Trypsin inhibitor Activity and beta-conglycinin Activity
TI and antigen protein in a test sample of trypsin inhibitory factor and antigen protein are quantitatively detected by adopting an ELISA method, and the kit adopts an indirect competition method. Because the kit is greatly influenced by the room temperature, the temperature of each test needs to be raised to 25 ℃ and the test reaction process is carried out in a biochemical incubator at constant temperature (37 ℃). Weighing a certain amount of sample (TI is 0.1g, antigenic protein is 0.3g, and the precision is 0.1mg) in a 50mL centrifuge tube, adding 30mL of extracting solution, extracting for 16h under oscillation at 25 ℃, standing for 2min, centrifuging at 4000r/min for 5min, taking supernatant, and taking supernatant and diluting by 70 times with 1 time of sample dilution working solution. Taking out the enzyme label strip, numbering the micropores corresponding to the standard sample of the sample box in sequence, making 2 holes for each sample and the standard sample in parallel, adding sample, washing the plate, adding enzyme label reagent, developing, adding stop solution, and reading OD value under 450/630nm double waves. According to the formula: absorbance was calculated as percent absorbance (%) — B/B0 × 100%, where B is the average absorbance value of the standard or sample and B0 is the average absorbance value of the standard (blank).
And (3) drawing a calibration curve: and drawing a standard curve by taking the percent absorbance of the standard as a vertical coordinate and the logarithm of the concentration of the standard as a horizontal coordinate, bringing the percent absorbance of the sample into the standard curve, reading out the corresponding concentration from the standard curve, and multiplying the corresponding concentration by a dilution coefficient to obtain the actual concentration of the TI and the antigen protein in the sample.
2. Detection of urease Activity
Refer to "determination of urease activity in soybean products for feeds" (GB/T8622-2006).
3. Detecting the content of total pentosan and soluble pentosan
Refer to spectrophotometry for measuring pentosan content in grains (NY/T2335-2013).
4. Detecting phytic acid content
Reference is made to spectrophotometry for determining the activity of a phytase for feed (G B/T18634-2009)
5. Detecting the content of acid soluble protein
The method for measuring the nitrogen content of the acid soluble protein comprises the following steps: weighing 2.00g of sample dry sample, adding 10ml of 15% trichloroacetic acid (TCA) solution, mixing uniformly, and standing for 5 min. Quantitatively transferring the solution, centrifuging for 10min at 4000r/min, taking 5ml of supernatant, placing into a Kjeldahl flask, and determining the nitrogen content by a Kjeldahl method, wherein the specific operation and calculation refer to GB/T6432-1994.
Table 1 comparison of results of examples of different treatment methods
As can be seen from table 1, the processing methods of examples 1, 2, 3, 4 and 5 are prominent compared to the blank soybean meal. Most preferably, the degradation rate of trypsin inhibitor is 79.46%, the degradation rate of beta-conglycinin is 91.81%, the urease activity is reduced to 0.024, the content of total pentosan is increased by 29.63%, the content of soluble pentosan is increased by 286.67%, the content of phytic acid is reduced by 30.07%, and the content of acid soluble protein is increased by 567.66%.
Example 7
Selecting 60 healthy Du growing ternary weaned piglets, wherein each 10 piglets form a group, and each group is repeated three times. The experimental groups were: the feeding insect protein obtained in example 1 was added to the feed for piglets at an addition of 10%, and the control group was: the ordinary feed containing the feeding insect protein obtained in example 1 was not added, and the other conditions were the same. The results show that the piglet feed intake is improved by 5.7 percent compared with the control group, the daily gain is improved by 8.5 percent, and the feed conversion ratio is reduced by 2.6 percent. As shown in table 2 below.
TABLE 2 influence of different treatment groups on growth Performance index of weaned piglets
Example 8
Yellow-feathered broiler farms with the same breeding level and internal and external environments are selected and divided into 3 groups, and each group has 1000 feathers. The experimental group is that the feeding insect protein obtained in the embodiment 1 is added into the broiler feed according to the addition amount of 8%; the control group was a normal diet without the protein concentrate obtained in example 1, and the other feeding conditions and the management level were the same. The results show that the feed intake of the experimental group is improved by 13.7 percent compared with the control group, the average feed conversion ratio is reduced by 5.3 percent, and the marketing rate is improved by 4.8 percent.
TABLE 3 influence of different treatment groups on the growth Performance index of yellow-feathered broilers
Example 9
The method comprises the steps of directly treating a mixture of 90g of cottonseed meal and 10g of corn meal by using hermetia illucens (namely, the treatment method of the embodiment 3), adjusting the water content to be 70%, controlling the material temperature to be 30-35 ℃, and reducing the total gossypol, free gossypol and combined gossypol contents (calculated by dry matters) of a treated group by 40.87%, 90.78% and 36.13% respectively compared with an untreated group after treating for 10 days. The protein content of the treated group is increased by 5.2 percent, the content of crude fat and crude fiber is obviously reduced, the content of peptide substances in the substrate is increased, the content of acid-soluble protein is respectively increased by 7.72 percent, 1.01 percent and 1.64 percent relative to the molecular mass of 18000 mu with the molecular mass of less than 600, 600-1000 and 1000-18000 mu, and the content of essential amino acids threonine, valine, isoleucine, leucine, phenylalanine and lysine is respectively increased by 11.51 percent, 9.25 percent, 27.58 percent, 6.43 percent, 15.50 percent and 12.62 percent.
TABLE 4 Total, free and bound gossypol contents (air-dried basis)
Table 5 conventional nutrient content (dry matter basis,%)
TABLE 6 percentage of peptides in different molecular weight ranges on feed stocks (% dry matter basis)
Table 7 amino acid content changes (dry matter basis,%)
Example 10
Respectively pre-fermenting the full-fat soybean, the rapeseed meal, the cottonseed meal and the peanut meal by using the enzyme-added composite strain, then throwing insect larvae into the mixture, and screening the insects or drying the insects and the insect residues together after the treatment is finished to obtain the protein raw material for feeding. Specifically, as shown in table 2. After the treatment, the contents of trypsin inhibitor, antigenic protein activity (including beta-conglycinin activity), urease activity, total pentosan and soluble pentosan, phytic acid, acid soluble protein, isothiocyanate, oxazolidinethione, free gossypol, tannin and saponin before and after the plant protein raw material is treated are measured.
Table 8: method setting for treating plant protein raw material by using hermetia illucens, tenebrio molitor and enzyme-added fermentation strain
TABLE 9 Total Soy anti-nutritional factor and content Change before and after treatment of nutritional component
TABLE 10 change in the content of rapeseed meal before and after anti-nutritional factor treatment
TABLE 11 content Change of cottonseed meal before and after anti-nutritional factor treatment
TABLE 12 content variation of peanut meal before and after anti-nutritional factor treatment
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A method for processing plant anti-nutritional factors by using insect larvae is characterized in that larval eggs of black soldier or yellow mealworms are added into feed, the temperature of materials is controlled to be 28-35 ℃ in the breeding process of the black soldier fly or yellow mealworm larvae, after the breeding time is 8-15 days, the black soldier fly larvae and the materials are dried at the temperature of 80 ℃, and the content of each anti-nutritional factor is detected.
2. The method of claim 1, wherein the feed is treated by using complex enzyme in combination with black water or tenebrio molitor larvae, and comprises the following steps: treating the feed by using the complex enzyme, then adding larvae of the black soldier fly or the yellow mealworm, controlling the temperature of the material to be 28-35 ℃ in the breeding process of the black soldier fly or the yellow mealworm larvae, drying the black soldier fly larvae and the material at 80 ℃ after the breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
3. The method for treating a plant anti-nutritional factor using insect larvae according to claim 2, wherein the complex enzyme comprises phytase and cellulase in an amount of 100IU/g phytase and 200IU/g cellulase.
4. A method of using insect larvae for the treatment of plant anti-nutritional factors according to claim 1, wherein the feed is treated with a combination of a composite bacterial species with larvae of black water or yellow meal worm, in particular: firstly, fermenting the composite strain to process feed, then adding larva eggs of the black soldier fly or the yellow mealworm, controlling the temperature of the material to be 28-35 ℃ in the breeding process of the black soldier fly or the yellow mealworm larvae, drying the black soldier fly larvae and the material at 80 ℃ after the breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
5. The method of claim 4, wherein the complex bacterial species are Saccharomyces boulardii, Bacillus subtilis, Lactobacillus plantarum, Bacillus natto, Saccharomyces cerevisiae, Lactobacillus casei; the mass ratio of each component is respectively 0.125 percent of Saccharomyces boulardii, 0.03 percent of Bacillus subtilis, 0.1 percent of Lactobacillus plantarum, 0.03 percent of Bacillus natto, 0.25 percent of beer yeast and 0.05 percent of Lactobacillus casei.
6. The method of claim 1, wherein the feed is treated by using the complex enzyme, the complex strain and the larvae of the black water or the tenebrio molitor in combination, specifically: firstly, pre-fermenting soybean meal by using a complex enzyme and a complex strain, then adding larvae eggs of black soldier or tenebrio molitor, controlling the material temperature at 28-35 ℃ in the breeding process of black soldier fly or tenebrio molitor larvae, drying the black soldier fly larvae and the material at 80 ℃ after the breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
7. The method of claim 1, wherein the feed is treated by using the complex enzyme, the complex strain and the larvae of the black water or the tenebrio molitor in combination, specifically: and (3) simultaneously adding the complex enzyme, the composite strain and the larvae eggs of the hermetia illucens or the tenebrio molitor into the feed, controlling the temperature of the materials to be 28-35 ℃ in the breeding process of the hermetia illucens or the tenebrio molitor larvae, drying the hermetia illucens and the materials at 80 ℃ after the breeding time is 8-15 days, and detecting the content of each anti-nutritional factor.
8. A method as claimed in any one of claims 1 to 7, wherein the anti-nutritional factors include trypsin inhibitor, β -conglycinin, urease, phytic acid.
9. The method for treating a plant antinutritional factor with insect larvae according to any one of claims 1 to 7, wherein the feed comprises whole soybean, rapeseed meal, cottonseed meal, peanut meal, and corn meal.
10. A vegetable protein material produced by the method of claim 6 or 7 using insect larvae for the treatment of plant anti-nutritional factors.
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