CN109874939A - A kind of antibacterial fly-maggot protein and preparation method thereof - Google Patents
A kind of antibacterial fly-maggot protein and preparation method thereof Download PDFInfo
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- CN109874939A CN109874939A CN201910329019.3A CN201910329019A CN109874939A CN 109874939 A CN109874939 A CN 109874939A CN 201910329019 A CN201910329019 A CN 201910329019A CN 109874939 A CN109874939 A CN 109874939A
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The present invention discloses a kind of antibacterial fly-maggot protein and preparation method thereof, comprising the following steps: mixed bacteria liquid is mixed to obtain mixed material with culture bottom material；Immature engineering fly maggot is launched in mixed material, carries out one grade fermemtation, second order fermentation, three grade fermemtation.This technique can make full use of the powerful degradation capability of fly maggot external digestion, while also provide enhanced the collaboration synbiosis of fly maggot He its concomitance bacterium, and double fermentors is allowed to become more considerable to the enzymolysis of material.The antibacterial fly-maggot protein can be used as a kind of routine protein feedstuff and use in pig complete feed, other than it can provide proteinaceous nutrient, can also effectively control grice diarrhoea rate, the zinc oxide being substituted in pannage.
Description
Technical field
The present invention relates to breeding feed field, a kind of antibacterial fly-maggot protein and preparation method thereof is related generally to.
Background technique
Since 2015, the 200000000 tons of high pointes of breakthrough in the Feed Manufacturing total amount in China continuous 4 years, wherein protein raw material was made
For one of raw material main in feed, demand is huge, needs import about 60,000,000 tons of soybean and fish meal every year, it is contemplated that 2020
Year, China's protein raw material still has 50,000,000 tons of notches, therefore, develops and uses new good protein resource as feed industry
Extremely urgent new task.
Animalsderived feedstuffs (fish meal, plasma protein powder, dried porcine saluble, meat meal tankage) are with animal itself or animal slaughtering
Waste afterwards is the feedstuff of raw material production, due to deriving from animal itself, no matter from protein level and amino
It is all excellent protein resource from the point of view of acid composition.But since bacterial reproduction is very fast in process for it, and there are itself
The pathogen of carrying and virus can be transmitted to the huge hidden danger of healthy animal by food chain, therefore country is at present to animal derived
The use limitation of feed is increasingly stringenter, and part kind has forbidden using in feed, this is to albumen inherently in short supply
It is even more to make the matter worse for the feed industry of matter raw material, so it is imperative to open up new protein resource.
Insect is the animal of most species on the earth, more than a total of about 120 ten thousand kinds, China can look into see there are about more than 20 ten thousand kinds,
Wherein many kinds and its product utilize (such as silk, honey) by the mankind very early.Fly maggot is screwworm, polypide battalion
It supports that abundant, the speed of growth is fast, nutrition high conversion rate, and based on external digestion, both passes through the various enzymes in metabolite, association
Bacterium degradation foreign macromolecules nutriment, then internal utilization is absorbed by skin, therefore lead in protein raw material development of resources
In domain, the utilization of fly maggot and its metabolin is the direction for having very future.
For a long time, antibiotic prophylaxis Animal diseases are added in animal feed and promote growth, are industry wisdoms, but by
In the pathogenic bacteria resistance to drugs problem that antibiotic generates, restrictive policy has been put into effect in the use that China has been directed to antibiotic, this
In the case of, animal husbandry is badly in need of finding efficiency of new, safe without toxic side effect the substitute to ensure animal-breeding.
A kind of method that zinc oxide is effectively control piglet nutrition diarrhea is added in piglet preweaning feed and child care pig feed,
But enters soil with excrement since the use of high dose zinc oxide will lead to a large amount of Zn-ef ficiencies, and then influence achilles tendon tear and food
Safety, therefore the even implementation without zinc scheme of low zinc will become inevitable in piglet feedstuff formula, it is necessary to searching can effectively substitute oxygen
Change the feedstuff of zinc effect.
Therefore, the existing technology needs to be improved and developed.
Summary of the invention
In view of above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a kind of antibacterial fly-maggot protein and its preparation sides
Method, the antibacterial fly-maggot protein can be used as a kind of routine protein feedstuff and use in pig complete feed, in addition to that can mention
Outside for proteinaceous nutrient, grice diarrhoea rate, the zinc oxide being substituted in pannage, it is intended to solve can also be effectively controlled
Zinc oxide in existing pannage using high dose causes a large amount of Zn-ef ficiencies to enter soil with excrement, asks what soil polluted
Topic.
Technical scheme is as follows:
A kind of preparation method of antibacterial fly-maggot protein, wherein the following steps are included:
(1) take bacillus subtilis, bacillus licheniformis, bacillus coagulans, saccharomyces cerevisiae, Bacillus acidi lactici, Bifidobacterium and
Photosynthetic bacteria, culture transferring enters in fluid nutrient medium and cultivates respectively；Aforementioned several bacterium solutions are mixed to get mixed bacteria liquid again；
(2) mixed bacteria liquid is mixed into obtain mixed material with culture bottom material, mixed material water tariff collection is between 40 ~ 50%；
(3) immature engineering fly maggot is launched in mixed material, temperature is maintained between 28 ~ 32 DEG C, and relative humidity is maintained at 50 ~
60%, it is passed through oxygen, carries out one grade fermemtation；
(4) 37 ~ 42 DEG C are warming up to, relative humidity is maintained between 40 ~ 50%, is passed through oxygen, carries out second order fermentation；
(5) 35 ~ 38 DEG C are warming up to, relative humidity is maintained between 60 ~ 70%, carries out three grade fermemtation, this is in the process sealed shape
State.
The preparation method of the antibacterial fly-maggot protein, wherein in step (1), bacillus subtilis bacteria concentration >=109CFU/
ML, bacillus licheniformis >=108CFU/mL, bacillus coagulans >=108CFU/mL, saccharomyces cerevisiae concentration >=107CFU/mL, cream
Acidfast bacilli concentration >=107CFU/mL, Bifidobacterium >=107CFU/mL, photosynthetic bacteria >=105CFU/mL。
The preparation method of the antibacterial fly-maggot protein, wherein in step (2), mixed bacteria liquid accounts for culture bottom material weight
10~20%。
The preparation method of the antibacterial fly-maggot protein, wherein the culture bottom material is including following according to weight fraction meter
Raw material: 85 ~ 95 parts of dregs of beans, 3 ~ 8 parts of wheat brans, 1 ~ 3 portion of milk powder, 1 ~ 3 portion of brown sugar, 0.5 ~ 2 part of yeast extract；
Dregs of beans and wheat bran are cured and sterilized through high steam；Milk powder, brown sugar, yeast extract melt through superheated water, then with
Dregs of beans and wheat bran mixing after curing sterilizing.
The preparation method of the antibacterial fly-maggot protein, wherein in step (3), launch immature engineering fly maggot and mixture
The mass ratio of material is immature engineering fly maggot: mixed material=3 ~ 5:1.
The preparation method of the antibacterial fly-maggot protein, wherein be passed through in step (3), during this compressed air 0.8 ~
1.5m3/ h continues 10 ~ 16 hours, is stirred 0.5 ~ 1.5 hour every 0.5 ~ 1.5 hour to mixed material, mixing speed is
3~10rpm
The preparation method of the antibacterial fly-maggot protein, wherein be passed through in step (4), during this compressed air 1.5 ~
2.5m3/ h continues 32 ~ 40 hours, is kept stirring, and mixing speed is 10 ~ 20rpm；
The preparation method of the antibacterial fly-maggot protein, wherein in step (5), three grade fermemtation process continues 20 ~ 30 hours.
The preparation method of the antibacterial fly-maggot protein, wherein the preparation method is further comprising the steps of:
(6) mixed material is dried, it is cooling, it crushes；
In drying course, drying temperature is maintained between 90 ~ 95 DEG C, makes material moisture between 10 ~ 12%.
A kind of antibacterial fly-maggot protein, wherein the antibacterial fly-maggot protein includes fly maggot, careless bacillus, lichens gemma bar
Bacterium, bacillus coagulans, saccharomyces cerevisiae, Bacillus acidi lactici, Bifidobacterium, photosynthetic bacteria, culture bottom material and the fermentation for cultivating bottom material
Product.
The utility model has the advantages that a kind of antibacterial fly-maggot protein and preparation method thereof of the invention, in the preparation method, use is a variety of
Probiotic combinations simulate fly maggot metabolism environment, and cooperate immature engineering fly maggot, and paving is sprinkled upon the culture after high temperature high pressure process together
In bottom material, by three stage double fermentations, material is sent into dryer and carries out low temperature drying, last crushing packing.This technique
The powerful degradation capability of fly maggot external digestion can be made full use of, while the collaboration symbiosis for also providing enhanced fly maggot and its concomitance bacterium is made
With allowing double fermentors to become more considerable to the enzymolysis of material.The antibacterial fly-maggot protein can be used as a kind of conventional egg
White feedstuff uses in pig complete feed, other than it can provide proteinaceous nutrient, can also effectively control grice diarrhoea
Rate, the zinc oxide being substituted in pannage.
Specific embodiment
The present invention provides a kind of antibacterial fly-maggot protein and preparation method thereof, to make the purpose of the present invention, technical solution and effect
Fruit is clearer, clear, and the present invention is described in more detail below.It should be appreciated that specific embodiment described herein is only
Only to explain the present invention, it is not intended to limit the present invention.
A kind of preparation method of antibacterial fly-maggot protein provided by the present invention simulates fly maggot generation using a variety of probiotic combinations
It thanks to environment, and cooperates immature engineering fly maggot, spread in the culture bottom material after being sprinkled upon high temperature high pressure process together, it is dual by three stages
Material is sent into dryer and carries out low temperature drying, last crushing packing by fermentation.This technique can make full use of fly maggot to disappear in vitro
The powerful degradation capability changed, while the collaboration synbiosis of fly maggot He its concomitance bacterium is also provided enhanced, allow double fermentors to material
Enzymolysis becomes more considerable.
Specifically, the preparation method of the antibacterial fly-maggot protein, comprising the following steps:
(1) take bacillus subtilis, bacillus licheniformis, bacillus coagulans, saccharomyces cerevisiae, Bacillus acidi lactici, Bifidobacterium and
Photosynthetic bacteria, culture transferring enters in fluid nutrient medium and cultivates respectively；Aforementioned several bacterium solutions are mixed to get mixed bacteria liquid again；Wherein withered grass bud
Spore bacillus concentration >=109CFU/mL, bacillus licheniformis >=108CFU/mL, bacillus coagulans >=108CFU/mL, saccharomyces cerevisiae
Concentration >=107CFU/mL, acidi lactici concentration >=107CFU/mL, Bifidobacterium >=107CFU/mL, photosynthetic bacteria >=105CFU/mL。
In this step, mainly before formal production by each rejuvenation of spawn, spread cultivation, be fabricated to bacterium solution.Bacillus subtilis,
Bacillus licheniformis, bacillus coagulans, saccharomyces cerevisiae, Bacillus acidi lactici, Bifidobacterium and photosynthetic bacteria, which can use, on the market may be used
The strain bought, simulates the probiotic combinations of fly maggot metabolite, and the strain bought on the market also may be implemented collaboration symbiosis and make
With.It is further preferable that directly isolated and purified to obtain from fly maggot metabolite bacillus subtilis, bacillus licheniformis,
Bacillus coagulans, saccharomyces cerevisiae, Bacillus acidi lactici, Bifidobacterium and photosynthetic bacteria, this probiotic combinations better effect.
(2) mixed bacteria liquid is mixed into obtain mixed material with culture bottom material, mixed material water tariff collection is between 40 ~ 50%.
In this step, mixed bacteria liquid accounts for the 10 ~ 20% of culture bottom material weight.The culture bottom material is according to weight fraction meter, packet
Include following raw material: 85 ~ 95 parts of dregs of beans, 3 ~ 8 parts of wheat brans, 1 ~ 3 portion of milk powder, 1 ~ 3 portion of brown sugar, 0.5 ~ 2 part of yeast extract.The above raw material
It does not need to crush in advance, laminates too tight and cause ventilation poor first is that material carefully will cause very much, influence fly maggot and aerobe
Growth and breeding；Second is that precomminution process requirement purchases pulverizer, increase energy consumption and production cost.The above raw material need to be by going out
Bacterium processing, the mode of sterilization treatment can be heat sterilization.Dregs of beans and wheat bran are cured and sterilized through high steam；Milk
Powder, brown sugar, yeast extract melt through superheated water, then with curing sterilizing after dregs of beans and wheat bran mix.Using hot water melt milk powder,
Brown sugar, yeast extract, not only act as sterilization functions, and it is more uniform to be also convenient for culture bottom material each component mixing.
(3) immature engineering fly maggot is launched in mixed material, the mass ratio for launching immature engineering fly maggot and mixed material is
Immature engineering fly maggot: mixed material=3 ~ 5:1 carry out one grade fermemtation.
During this one grade fermemtation, immature fly maggot and yeast quasi-microorganism are mainly cultivated, saccharomycete is aerobic and more difficult culture,
Therefore the stage needs to be passed through oxygen and promotes its growth and breeding, but oxygen cannot be too many, otherwise bacillus understands mass propagation, ferment
Female bacterium can play enzymolysis in material, while generate fragrant and sweet smell；Immature fly maggot is also required to be grown up as early as possible to improve it
Vigor.During this, temperature is maintained between 28 ~ 32 DEG C, and relative humidity is maintained at 50 ~ 60%, be passed through compressed air 0.8 ~
1.5m3/ h continues 10 ~ 16 hours, is stirred 0.5 ~ 1.5 hour every 0.5 ~ 1.5 hour to mixed material, mixing speed is
3~10rpm。
(4) 37 ~ 42 DEG C are warming up to, relative humidity is maintained between 40 ~ 50%, carries out second order fermentation, this is passed through pressure in the process
1.5 ~ 2.5m of contracting air3/ h continues 32 ~ 40 hours, is kept stirring, and mixing speed is 10 ~ 20rpm.
During second order fermentation, it is main cultivate in big fly maggot and aerobic spore-bearing bacilli quasi-microorganism, while during the stage is
The growth phase of big fly maggot.Saccharomycete has been bred in the stage, therefore increasing ventilation keeps bacillus quasi-microorganism fast
Speed increases, to make full use of efficient enzymolysis of the bacillus to material.
(5) 35 ~ 38 DEG C are warming up to, relative humidity is maintained between 60 ~ 70%, carries out three grade fermemtation, this is in the process closed
State continues 20 ~ 30 hours without stirring.
It is main to cultivate lactic acid producing quasi-microorganism during three grade fermemtation, before aerobe sufficiently breed, the stage
Into anaerobic fermentation stage, stuffiness, without stirring for the microorganism of galactopoiesis acids mainly accumulates organic acid, accumulates bioactivity
Substance improves material palatability.
(6) mixed material is dried, it is cooling, it crushes.
In drying course, drying temperature is maintained between 90 ~ 95 DEG C, makes material moisture between 10 ~ 12%.After drying
Material is crossed 30 meshes and is crushed after being cooled to room temperature.
A kind of antibacterial fly-maggot protein is also provided in the present invention, the antibacterial fly-maggot protein uses preparation method as described above
It is prepared.The antibacterial fly-maggot protein includes fly maggot, careless bacillus, bacillus licheniformis, bacillus coagulans, wine brewing ferment
Mother, Bacillus acidi lactici, Bifidobacterium, photosynthetic bacteria, culture bottom material and the tunning for cultivating bottom material.The antibacterial fly-maggot protein can
It, can be with other than it can provide proteinaceous nutrient to be used in pig complete feed as a kind of routine protein feedstuff
Effectively control grice diarrhoea rate, the zinc oxide being substituted in pannage.
Microorganism is used only as fermentor in general fermentation system currently on the market, and biological glycolysis effect is limited；And this
Fly maggot and probiotics has then been used to carry out glycolysis to material as double fermentors in scheme of the invention, wherein fly maggot is by breeding
The engineering fly maggot of domestication, fertility is strong, growth is rapid, and probiotics is that basis is isolated and purified from fly maggot metabolin concomitance bacterium
Specific effective microorganisms, choose a variety of strain combinations, simulate fly maggot metabolism environment, cooperateing with for these types of strain and fly maggot is total
Raw ability is strong, therefore double fermentation system glycolysis are deep, and anti-nutritional factors removal rate is higher, the tunning accumulated in product
More.Through detecting, fly-maggot protein is seen below in detail compared with the data of common fermentation dregs of beans (the fermented bean dregs product that market is bought)
Table 1.
Table 1
Fermentation system in feed industry at present generally uses the solid fermentation method of relatively simple fermentation vat or fermentation tank, right
The state modulator of material is very simple and crude, and external environment variation influences very greatly product final quality.To fermentation in the present invention program
Step optimizes, and whole process is made to can be used full-automatic fermentation method, material from sterilizing, inoculation fermentation device, solid fermentation to
Drying crushes, and whole process can realize that automated production, especially solid fermentation stage are divided into three sections of zymotechniques, for every
The physilogical characteristics of a stage fly maggot and different microorganisms carry out specific accurate parameter fermentation, and external environment changes to the final product of product
Matter influences less, therefore fermentation pattern is more advanced, and ferment effect is outstanding.
General antibacterial fly-maggot protein is pure fly maggot polypide product, and at high cost, product composition and trophic component are more single
One.And using the antibacterial fly-maggot protein of the present invention program production is a kind of compound protein, cost is relatively low, and the program is sufficiently sharp
With the powerful external digestion ability of fly maggot, the high molecular weight protein in plant compost is degraded to the peptide egg that animal is easy to absorb
It is white, the potentiality of plant material are sufficiently excavated, while by the detection to product, discovery can also accumulate such as antibacterial in the product
The bioactive substances such as peptide, agglutinin, chitosan, glutathione, free amino acid, therefore nutrients in the antibacterial fly-maggot protein
Matter is easy to be absorbed by animal, but also has the function of certain disease-resistant and maintenance intestinal health.
A kind of antibacterial fly-maggot protein is also provided in the present invention, the antibacterial fly-maggot protein uses preparation method as described above
It is prepared.The antibacterial fly-maggot protein belongs to functional protein raw material, can effectively control pig trophism diarrhea, can be used for
Zinc oxide is substituted, the additive as feed is particularly suitable for the additive of pannage.And antibacterial fly maggot provided by the present invention
Albumen mainly have plant material, animal protein raw material and probiotic group at, it is environmentally friendly, even if large dosage using will not
Adverse effect is caused to environment, food.
Below by way of specific embodiment, the invention will be further described.
Embodiment 1
The production process of antibacterial fly-maggot protein is as follows:
Take bacillus subtilis, bacillus licheniformis, bacillus coagulans, saccharomyces cerevisiae, Bacillus acidi lactici, Bifidobacterium and photosynthetic
Bacterium, culture transferring enters respectively cultivates for 24 hours in fluid nutrient medium, and it is spare after mixing to be finally delivered to storage bacterium tank, wherein withered grass gemma
Bacillus concentration >=109CFU/mL, bacillus licheniformis >=108CFU/mL, bacillus coagulans >=108CFU/mL, saccharomyces cerevisiae are dense
Degree >=107CFU/mL, acidi lactici concentration >=107CFU/mL, Bifidobacterium >=107CFU/mL, photosynthetic bacteria >=105CFU/mL。
First 4 hours of formal production, it is spare to collect the immature engineering fly maggot just hatched from fly maggot breeding workshop.
Culture bottom material is made of 90% dregs of beans, 5% wheat bran, 2% milk powder, 2% brown sugar, 1% yeast extract, does not need precomminution.
Wherein dregs of beans and wheat bran are first delivered to sterilization tank, are passed through 115 DEG C of high steam 8min and are cured and sterilized.Remaining ingredient
Ingredient is prepared after being melted with hot water in (i.e. milk powder, brown sugar, yeast extract), send spare to fluid reservoir.
Material after curing is delivered to cooling tank, cooling processing is carried out to it by condensed water and compressed air, to material
Temperature is down to 40 DEG C or so, is transported to breeding tank, sprays proportion liquid by pipeline, supplies culture bottom material composition, while
Bacterium solution is sprayed onto material by pipeline, mixed bacteria liquid accounts for the 15% of culture bottom material weight.It cultivates bottom material and bacterium solution is abundant in tank
It is uniformly mixed, it is ensured that material moisture is between 40 ~ 50%.
The material stirred evenly is delivered to fermentor and carries out one grade fermemtation, which mainly cultivates immature fly maggot and yeast
Quasi-microorganism.Immature engineering fly maggot is launched according to fly maggot/wet stock 3:1 ratio, and is passed through compressed air 1.0m3/ h continues
12 hours, temperature was maintained between 28 ~ 32 DEG C in fermentor, and relative humidity is maintained between 50 ~ 60%, was started and is stirred every 1 hour
It mixes machine to be stirred material 1 hour, 5 rpm.
Second order fermentation is carried out after one grade fermemtation, the stage mainly cultivate in big fly maggot and aerobic spore-bearing bacilli class it is micro-
Biology.Temperature remains between 37 ~ 42 DEG C in fermentor, and relative humidity is maintained at 40 ~ 50%, is passed through 2.0 m of compressed air3/ h,
Continue 36 hours, starts blender and material is stirred, 15 rpm.
Three grade fermemtation is carried out after second order fermentation, which mainly cultivates lactic acid producing quasi-microorganism.Temperature in fermentor
It remains between 35 ~ 38 DEG C, relative humidity is maintained at 60 ~ 70%, and any gas is not passed through into tank, continues 24 hours, closed hair
Fermentation tank is not turned on blender.
Three grade fermemtation terminates material and is transported to low temp dryer by auger to be dried, and dries room temperature and is maintained at 90 ~ 95
Between DEG C, material moisture is between 10 ~ 12% after drying.
Material after drying is delivered to cooler to cool down, feeding pulverizer crosses 30 mesh powder after being cooled to room temperature
It is broken, obtain the antibacterial fly-maggot protein.
The ingredient of antibacterial fly-maggot protein is detected, testing result is as follows:
Moisture < 10%；Crude protein >=55%；Lysine >=3.1%；Organic acid >=3.0%；Small peptide >=12%；Antibacterial peptide >=0.15 ‰；
Glutathione >=40mg/kg；Total amount of probiotics >=1.0 × 106CFU/g。
Zoopery situation is as follows:
Tested on Guangdong pig farm, healthy miscellaneous child care pig 120 of the ternary of 9kg or so selected to be randomly divided into 2 groups --- control
Group and test group, every group of 6 repetitions, each repetition 10.
The common child care swine rations that control group fed is prepared based on Corn-soybean-fish meal-dried porcine saluble, and day
0.2 ‰ olaquindoxes, 0.12 ‰ virginiamycins, 2.8 ‰ zinc oxide are added in grain, olaquindox and virginiamycin are anti-
Raw element.
Test group daily ration then replaces 4% whole fish meal, whole on the basis of control group daily ration using 7% antibacterial fly-maggot protein
Dried porcine saluble and part whey powder do not add olaquindox, virginiamycin, zinc oxide in test group.Pair of two kinds of daily rations
Than as shown in table 2 below.
Table 2
Two kinds of diets of different nutrition level are suitable, and test period is 30, manage and carry out according to daily pig farm.
Test result show test group average daily gain 16 grams more than control group/head/day (P < 0.05), improve
5.1%；Feed intake 24 grams more than control group/head/day, improve 4.3%；Whole feedstuff-meat ratio reduces 0.02 compared to the control group；It is right
Diarrhea rate according to group and test group is respectively 4.0% and 3.7%, is not significantly different (P > 0.05)；Control group and test group it is dead
Naughty rate is respectively 3.0% and 2.8%, is not significantly different (P > 0.05).Illustrate to use 7% antibacterial fly maggot egg in child care swine rations
White, the body aggregate velocity of child care pig is accelerated, and abilities of digestive and absorption is stronger, and can substitute antibiotics and zinc oxide completely
Effect, therefore the antibacterial fly-maggot protein is a kind of functional protein raw material.
It should be understood that the application of the present invention is not limited to the above for those of ordinary skills can
With improvement or transformation based on the above description, all these modifications and variations all should belong to the guarantor of appended claims of the present invention
Protect range.
Claims (10)
1. a kind of preparation method of antibacterial fly-maggot protein, which comprises the following steps:
(1) take bacillus subtilis, bacillus licheniformis, bacillus coagulans, saccharomyces cerevisiae, Bacillus acidi lactici, Bifidobacterium and
Photosynthetic bacteria, culture transferring enters in fluid nutrient medium and cultivates respectively；Aforementioned several bacterium solutions are mixed to get mixed bacteria liquid again；
(2) mixed bacteria liquid is mixed into obtain mixed material with culture bottom material, mixed material water tariff collection is between 40 ~ 50%；
(3) immature engineering fly maggot is launched in mixed material, temperature is maintained between 28 ~ 32 DEG C, and relative humidity is maintained at 50 ~
60%, it is passed through oxygen, carries out one grade fermemtation；
(4) 37 ~ 42 DEG C are warming up to, relative humidity is maintained between 40 ~ 50%, is passed through oxygen, carries out second order fermentation；
(5) 35 ~ 38 DEG C are warming up to, relative humidity is maintained between 60 ~ 70%, carries out three grade fermemtation, stuffiness during this.
2. the preparation method of antibacterial fly-maggot protein according to claim 1, which is characterized in that in step (1), withered grass gemma
Bacillus concentration >=109CFU/mL, bacillus licheniformis >=108CFU/mL, bacillus coagulans >=108CFU/mL, saccharomyces cerevisiae are dense
Degree >=107CFU/mL, acidi lactici concentration >=107CFU/mL, Bifidobacterium >=107CFU/mL, photosynthetic bacteria >=105CFU/mL。
3. the preparation method of antibacterial fly-maggot protein according to claim 1, which is characterized in that in step (2), mixed bacteria liquid
Account for the 10 ~ 20% of culture bottom material weight.
4. the preparation method of antibacterial fly-maggot protein according to claim 2, which is characterized in that the culture bottom material is according to weight
Measure score meter, including following raw material: 85 ~ 95 parts of dregs of beans, 3 ~ 8 parts of wheat brans, 1 ~ 3 portion of milk powder, 1 ~ 3 portion of brown sugar, 0.5 ~ 2 part of yeast leaching
Cream；
Dregs of beans and wheat bran are cured and sterilized through high steam；Milk powder, brown sugar, yeast extract melt through superheated water, then with
Dregs of beans and wheat bran mixing after curing sterilizing.
5. the preparation method of antibacterial fly-maggot protein according to claim 1, which is characterized in that in step (3), launch immature
The mass ratio of engineering fly maggot and mixed material is immature engineering fly maggot: mixed material=3 ~ 5:1.
6. the preparation method of antibacterial fly-maggot protein according to claim 1, which is characterized in that in step (3), during this
It is passed through 0.8 ~ 1.5m of compressed air3/ h continues 10 ~ 16 hours, 0.5 is stirred to mixed material every 0.5 ~ 1.5 hour ~
1.5 hours, mixing speed was 3 ~ 10rpm.
7. the preparation method of antibacterial fly-maggot protein according to claim 1, which is characterized in that in step (4), during this
It is passed through 1.5 ~ 2.5m of compressed air3/ h continues 32 ~ 40 hours, is kept stirring, and mixing speed is 10 ~ 20rpm.
8. the preparation method of antibacterial fly-maggot protein according to claim 1, which is characterized in that in step (5), three grade fermemtation
Process continues 20 ~ 30 hours.
9. the preparation method of antibacterial fly-maggot protein according to claim 1, which is characterized in that the preparation method further includes
Following steps:
(6) mixed material is dried, it is cooling, it crushes；
In drying course, drying temperature is maintained between 90 ~ 95 DEG C, makes material moisture between 10 ~ 12%.
10. a kind of antibacterial fly-maggot protein, which is characterized in that the antibacterial fly-maggot protein includes fly maggot, careless bacillus, lichens bud
Spore bacillus, saccharomyces cerevisiae, Bacillus acidi lactici, Bifidobacterium, photosynthetic bacteria, cultivates bottom material and cultivates bottom material bacillus coagulans
Tunning.
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