CN106306359A - Method for preparing feed for grass feed animal through straw fermentation - Google Patents
Method for preparing feed for grass feed animal through straw fermentation Download PDFInfo
- Publication number
- CN106306359A CN106306359A CN201610688627.XA CN201610688627A CN106306359A CN 106306359 A CN106306359 A CN 106306359A CN 201610688627 A CN201610688627 A CN 201610688627A CN 106306359 A CN106306359 A CN 106306359A
- Authority
- CN
- China
- Prior art keywords
- straw
- fermentation
- feed
- grass
- stalk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for preparing feed for grass feed animals through green straw fermentation. The method is technically characterized in that after rice straws, wheat straws, corn straws and the like are subjected to steam explosion and ammonification treatment, bovine rumen compound bacteria are used in the anaerobic fermentation, and a proper amount of azolla filliculoidas powder is added in a fermentation product to serve as a nutrient supplement. The added azolla filliculoidas is wide in adaptability, large in increment and high in the crude protein content of stems and leaves, and is very suitable for solving the problem of insufficiency of the crude protein of supplementing straw fermentation feed. A final product of the project is high in crude protein content and is rich in multiple essential amino acids and multiple microelements of calcium, iron, zinc, selenium and the like; the fragrant green straw fermentation feed for the grass feed animal is used for feeding animals with high feed conversion and delicious meat, such as a cow, a sheep and a sika deer. Therefore, the method provided by the technical invention is a new power for promoting efficient cyclic utilization of the straw and promoting the prosperity and the development of beef in the southern part of China, and has a bright prospect of industrialized application.
Description
Technical field
The present invention relates to field of feed processing, particularly relate to agricultural crop straw and high quality forage as raw material, with bovine rumen
Microorganism species is that animal feed preparation method raised by a kind of stalk fermentation grass of anaerobic fermentation bacterial.
Background technology
China is large agricultural country, can produce various straw more than 700,000,000 ton every year, account for whole world straw total amount 20%～
30%, wherein, the cereal crops straw about 5.8 hundred million tons such as Oryza sativa L., Semen Tritici aestivi, corn and soybean, potato class, account for the 89% of straw total amount；Along with
The comprehensive level of production of Chinese agriculture constantly improve, China's straw total output is generally in the gesture of constantly growth.Therefore, China's straw
The approach of stalk cycling and reutilization and method are the big technical problems of received much concern for many years.In recent years, along with crop production water
The raising of gentle multiple crop index, using chemical fertilizer in a large number, the superfluous packing phenomenon of straw is more prominent, in busy farming season, and agriculture
Field crop straw burning is serious, causes serious environmental pollution, even has influence on aviation safety.Therefore, the efficient utilization of straw is carried out
Can be that positive contribution is made in the sustainable development realizing national economy.
The rice growing area of China accounts for the second in the world, and yield accounts for the first in the world.According to statistics, domestic product water in 2008
Rice about 1.9 hundred million tons, is calculated by ratio of grain to straw 1:1, can produce the Caulis et Folium Oryzae of about 1.9 hundred million tons simultaneously, accounts for whole straw quantity and surpasses
Cross 30 %, become the straw feed resource that agriculture district is maximum.China's the third-largest beef cattle band south China beef cattle band is in Oryza sativa L. main product
District, fully develops straw resource, can alleviate the restriction that this district's cultivated area is few and natural meadow is not enough, promote plant-eating animal
The large-scale development produced, reduces the problem of environmental pollution that crop straw burning causes simultaneously.
China's wheat stalk annual production reaches 15.6 hundred million tons, accounts for about the 15% of agricultural crop straw total amount.Wheat stalk is also potential
One of feedstuff, it contains the materials such as substantial amounts of carbohydrate, protein, cellulose, hemicellulose.But, Semen Tritici aestivi
Straw processes and equally exists a lot of problem.Therefore, the problem that wheat straw is also our overriding concern at present is made full use of.
China's corn stalk annual production reaches 2.5 hundred million tons, accounts for about the 25% of agricultural crop straw total amount.Corn straw contains rich
Rich nutrition and available chemical composition, can be used as the raw material of livestock feed.For a long time, corn straw is exactly domestic animal
One of raw material of main roughage.Relevant result of laboratory test shows, corn straw contains carbohydrate, 2%～4% of more than 30%
Protein and the fat of 0.5% 1%, both can ensiling, it is possible to Direct-fed.For herbivore, the corn straw of 2 kg
Net energy for gain is equivalent to the corn kernel of 1kg, particularly after ensiling, yellow storage, ammonification and saccharifying etc. process, can improve utilization
Rate, benefit will be more considerable.Analyzing according to the study, digestible energy contained in corn straw is 2 235.8kJ/kg, and nutritious, always
Energy is suitable with herbage.Corn straw is carried out retrofit process, is fabricated to high nutrition animal feeding-stuff, not only contribute to development
Animal husbandry, and cross abdomen also field by straw, have more good ecological benefits and economic benefit.
The blade of Caulis et Folium Oryzae and the surface of stem stalk are with substantial amounts of silicified cell and waxiness.It is unfavorable for that it is disappeared by grass raising property animal
Changing, nutritive value is relatively low.Using steam explosion is conventional Caulis et Folium Oryzae preprocess method, can significantly destroy the ultra micro knot of Caulis et Folium Oryzae
Structure, increases the speed of fermentable.
In order to solve the CN of Caulis et Folium Oryzae than higher short slab, using ammoniated treatment is well to select, the straw after ammoniated treatment
Stalk, its lignocellulose is destructurized, and cellulose crystallite district is destroyed further, beneficially microbial degradation cellulose；Simultaneously
The CN ratio of Caulis et Folium Oryzae is remarkably decreased, then processes through microbe fermentation method, can be effectively improved the crude protein content of straw feed, reduces thick
Fiber content.
Straw, corn stalk organizational structure the most sufficiently complex, it is also desirable to have the processing method being similar to Caulis et Folium Oryzae.Middle promulgated by the State Council
The bright patent application method (CN201310120218.6) of albumen feedstuff " the Neuraspora crassa solid fermentation Caulis et Folium Oryzae prepare " provides
Caulis et Folium Oryzae is mixed by one with fluid medium, prepares solid-state fermentation culture medium；Access thick again in solid-state fermentation culture medium
Rough neurospora carries out the method for solid fermentation, and the method not only fluid medium cost is high, and uses Neuraspora crassa solid-state
Whether Rice Straw Fermented produces that grass is raised the disadvantageous by-product of animal health is the most unclear.Therefore there is new approach.Research confirms
The cud of the ruminant such as cattle, sheep contains abundant ligocellulose degradation's microorganism, therefore micro-to ruminant tumor gastric
Biological research is increasingly becoming the new direction exploring the effective degradation pathway of cellulose.
The adaptability of food leaf grass is very wide, and the crude protein content of its stem and leaf is up to 34%, additionally contains a large amount of vitamin, ammonia
Multiple beneficial trace element and the mineral such as base acid, SOD, potassium, selenium, chlorophyll, rheum emodin, be that kind of novel high protein is herded
Grass.Food leaf grass is used for developing grain-saving type albumen feedstuff, improves meat, fowl, egg, milk quality, can ensure animal husbandry healthy aquaculture.In
State's patent of invention " preparation method of a kind of novel cattle and sheep feedstuff " proposes eats leaf grass and Eupatorium cannabinum L. (Eupatorium coelestinum L.) mixed fodder, but in a large number
Using food leaf grass not only cost height but also waste nutrition, the supplementary as straw feed is then well suited with one's place.
Summary of the invention:
It is an object of the invention to solve the problems referred to above, it is provided that animal feed preparation method raised by a kind of stalk fermentation grass.
Present invention process step is as follows:
Step 1, straw steam explosion: choose clean, do not have the straw gone mouldy, and cuts into length 1-10 centimetre interior
Particle, puts in steam explosion equipment, is passed through high-temperature steam, reaction condition control reaction pressure 2.0 MPa ~ 3.5MPa,
At reaction temperature 110 DEG C ~ 130 DEG C after response time 20 min ~ 40 min, moment pressure release so that the cellulose in straw, half fibre
Dimension element comes out.Described straw is Caulis et Folium Oryzae, straw, corn stalk；
Step 2, straw ammoniation:
Taking step 1 straw steam explosion stalk powder 100 parts to load in plastic barrel, spraying into mass fraction wherein is 22.5%
Strong aqua ammonia 15 parts, tightens bag mouth, static 48 ~ 70 hours, standby；
Step 3, bovine rumen microorganism species fermentation culture；
In the seed tank that volume is 15L, load anaerobe liquid culture medium 10L, put in microorganism fermentation tank, go out
Bacterium, be cooled to after room temperature inoculate bovine rumen microorganism species Anaerobic culturel original seed 1L, fermentation temperature control 20 DEG C～50 DEG C,
Make microorganism Fast-propagation to viable count 1x10 under the anaerobic environment of pH6.5 ~ 7.010CFU/mL.According to production scale, can be by
According to the ratio of inoculum concentration 10% ~ 15%, bigger fermentation tank expands the cultivation of bovine rumen microorganism species further.
Described bovine rumen microorganism species system inventor butchers to separate rear rumen content from healthy and strong beef cattle and obtains
, wherein major microorganisms kind include Ruminococcus albus (Ruminococcusalbus), Butyrivibrio fibrisolvens
(Butyrivibriofibrisolvens), yeast (Saccharomyces), Trichoderma spp. (Trichoderma spp), four kinds are micro-
Bion ratio is about 4:4:1:1；
Described anaerobe liquid culture medium method of completing the square is as follows: seven water disodium hydrogen phosphate 64g, potassium dihydrogen phosphate 15g, chlorine
Changing sodium 2.5g, ammonium chloride 5.0g, microcrystalline Cellulose 10g, the reed powder 20g of beef extract 5g, excessively 200 mesh sieves, constant volume is 1L.
Through 121 DEG C of sterilizings, standby；
Step 4, the fermentation culture opening steps 3 Plastic Drum spiral cover of ammoniated straw, quickly connect in step 3 ammoniated straw finished product
Planting bovine rumen microorganism species anaerobic cultures, inoculum concentration is equivalent to the 10% ~ 15% of stalk powder weight；Add simultaneously and be equivalent to
The Sal of powder of straw weight 0.45% ~ 0.55%, spins lid, rolls Plastic Drum and promotes content mix homogeneously, lucifuge fermentation 15
My god ~ 30 days；
Prepared by step 5, food leaf grass meal: gather in fresh food leaf grass blade leaf, in 120 DEG C of 5min ~ 10min that complete, 60 DEG C ~ 80 DEG C heat
Wind is dried to moisture content 8% ~ 12%(butt), it is crushed to 40 mesh ~ 60 mesh, standby；
Animal feed allotment raised by step 6, a kind of stalk fermentation grass: take the powder of straw 70 of step 4 fermenting-ripening under clean environment
Part ~ 90 parts；Step 5 eats leaf grass meal 10 parts ~ 30 parts, is sufficiently mixed uniformly, loads in big plastic bag and tighten bag mouth so that it is continue slow
Slowly fermenting 5 days ~ 10 days, obtains green stalk fermentation type grass the raising property animal feed of fragrant.
After the invention have the advantages that Caulis et Folium Oryzae, straw, corn straw etc. are used steam explosion, ammoniated treatment by the technology of the present invention, adopt
Carrying out primary fermentation with bovine rumen composite flora, tunning adds appropriate food leaf grass meal again as nutrition enhancer.
The present invention is with Caulis et Folium Oryzae as primary raw material, after Caulis et Folium Oryzae uses steam explosion, ammoniated treatment, uses bovine rumen compound bacteria
Group carries out anaerobic fermentation, substantially increases Caulis et Folium Oryzae and grass is raised the nutritive value of animal, and the increment utilization for Caulis et Folium Oryzae have found newly to go out
Road；Food leaf grass strong adaptability, the increment added in the present invention are big, protein content is up to 34%, are highly suitable for supplementing Caulis et Folium Oryzae
The problem that the protein component of feedstuff is not enough；The final products of this project are that crude protein content is high, rich in multiple essential amino acids
And the various trace elements such as calcium, ferrum, zinc, selenium, animal fermented feedstuff raised by the green grass of fragrant.It is for raising cattle and sheep
The price of deed is high, delicious meat, therefore can become the power promoting south China beef cattle band prosperity and development, therefore has the product of light
Industry application prospect.
Specific embodiment
For making those skilled in the art understand production technology and the technique effect of the present invention in detail, below with concrete production
Example is further described application and the technique effect of the present invention.
Embodiment one: green rice straw fermented cattle feed
Step 1, Caulis et Folium Oryzae steam explosion: choose clean, do not have the Caulis et Folium Oryzae gone mouldy, and cuts into interior broken of length 10 centimetres
Material, puts in steam explosion equipment, is passed through high-temperature steam, reaction condition is controlled in reaction pressure 3.5MPa, reaction temperature 130
At DEG C after response time 40 min, moment pressure release so that cellulose, hemicellulose in Caulis et Folium Oryzae come out；
Step 2, rice straw powder ammonification:
100 parts of the steam explosion Caulis et Folium Oryzae powder taking step 1 loads in plastic barrel, and it is 22.5% dense for spraying into mass fraction wherein
Ammonia 15 parts, tightens bag mouth, static 70 hours, standby.
Step 3, bovine rumen microorganism species fermentation culture；
In the seed tank that volume is 15L, load anaerobe liquid culture medium 10L, put in microorganism fermentation tank, go out
Bacterium, be cooled to after room temperature inoculate bovine rumen microorganism species Anaerobic culturel original seed 1L, fermentation temperature control 20 DEG C～50 DEG C,
Make microorganism Fast-propagation to viable count 1x10 under the anaerobic environment of pH6.5 ~ 7.010CFU/mL.According to production scale, can be by
According to the ratio of inoculum concentration 10%, bigger fermentation tank expands the cultivation of bovine rumen microorganism species further；
Described bovine rumen microorganism species system inventor butchers to separate rear rumen content from healthy and strong beef cattle and obtains, the most micro-
Biological species include Ruminococcus albus (Ruminococcusalbus), Butyrivibrio fibrisolvens (Butyrivibriofibrisolvens),
Yeast (Saccharomyces), Trichoderma spp. (Trichoderma spp), four kinds of microorganism individuality ratios are about 4:4:1:1；
Described anaerobe liquid culture medium method of completing the square is as follows: seven water disodium hydrogen phosphate 64g, potassium dihydrogen phosphate 15g, chlorine
Changing sodium 2.5g, ammonium chloride 5.0g, microcrystalline Cellulose 10g, the rice straw powder 20g of beef extract 5g, excessively 200 mesh sieves, constant volume is 1L.
Through 121 DEG C of sterilizings, standby；
Step 4, the fermentation opening steps 3 Plastic Drum spiral cover of Ammoniated rice straw powder, quickly connect in step 3 Ammoniated rice straw powder finished product
Planting bovine rumen microorganism species anaerobic cultures, inoculum concentration is equivalent to the 15% of Ammoniated rice straw grain weight amount；Add simultaneously and be equivalent to straw
The Sal of stalk grain weight amount 0.55%, spins lid, rolls Plastic Drum and promotes content mix homogeneously, and lucifuge is fermented 30 days；
Prepared by step 5, food leaf grass meal: gathering in fresh food leaf grass blade leaf, at 120 DEG C of 10min that complete, 80 DEG C of hot air dryings are to containing
Water rate 12%(butt), it is crushed to 60 mesh, standby；
Step 6, rice straw fermented type cattle feed are allocated: take the rice straw powder 90 parts of step 4 fermenting-ripening under clean environment；Step
5 food leaf grass meals 30 parts, are sufficiently mixed uniformly, load in big plastics and tighten bag mouth so that it is continue slowly fermentation 10 days, obtain abnormal smells from the patient
Green rice straw fermented type grass the raising property animal feed of fragrance.
Embodiment two: green straw fermented type sheep feedstuff
Step 1, straw steam explosion: choose clean, do not have the straw gone mouldy, and cuts into 5 centimetres of interior particles of length,
Put in steam explosion equipment, be passed through high-temperature steam, reaction condition is controlled reaction pressure 3.0MPa, reaction temperature 120 DEG C
After lower response time 30 min, moment pressure release so that cellulose, hemicellulose in straw come out；
Step 2, straw powder ammonification:
Taking 100 parts of step 1 steam explosion straw powder to load in plastic barrel, spraying into mass fraction wherein is 22.5% dense ammonia
15 parts of water, tightens bag mouth, static 60 hours, standby；
Step 3, bovine rumen microorganism species fermentation culture；
In the seed tank that volume is 15L, load anaerobe liquid culture medium 10L, put in microorganism fermentation tank, go out
Bacterium, be cooled to after room temperature inoculate bovine rumen microorganism species Anaerobic culturel original seed 1L, fermentation temperature control 20 DEG C～50 DEG C,
Make microorganism Fast-propagation to viable count 1x10 under the anaerobic environment of pH6.5 ~ 7.010CFU/mL.According to production scale, can be by
According to the ratio of inoculum concentration 10%, bigger fermentation tank expands the cultivation of bovine rumen microorganism species further；
Described bovine rumen microorganism species system inventor butchers to separate rear rumen content from healthy and strong beef cattle and obtains, Qi Zhongzhu
Want microbe species include Ruminococcus albus (Ruminococcusalbus), Butyrivibrio fibrisolvens
(Butyrivibriofibrisolvens), yeast (Saccharomyces), Trichoderma spp. (Trichoderma spp), four kinds are micro-
Bion ratio is about 4:4:1:1；
Described anaerobe liquid culture medium method of completing the square is as follows: seven water disodium hydrogen phosphate 64g, potassium dihydrogen phosphate 15g, chlorine
Changing sodium 2.5g, ammonium chloride 5.0g, microcrystalline Cellulose 10g, the straw powder 20g of beef extract 5g, excessively 200 mesh sieves, constant volume is 1L.
Through 121 DEG C of sterilizings, standby；
Step 4, the fermentation opening steps 3 Plastic Drum spiral cover of ammonification straw powder, quickly in the ammonification straw powder finished product of step 3
Inoculation bovine rumen microorganism species anaerobic cultures, inoculum concentration is equivalent to the 12% of ammonification straw grain weight amount；Add simultaneously and be equivalent to
The Sal of powder of straw weight 0.5%, spins lid, rolls Plastic Drum and promotes content mix homogeneously, and lucifuge is fermented 20 days；
Prepared by step 5, food leaf grass meal: gathering in fresh food leaf grass blade leaf, at 120 DEG C of 7min that complete, 65 DEG C of hot air dryings are to containing
Water rate 10%(butt), it is crushed to 50 mesh, standby；
Step 6, rice straw fermented type cattle feed are allocated: take 80 parts of the straw powder of step 4 fermenting-ripening under clean environment；Step
Food leaf grass meal 20 parts prepared by 5, is sufficiently mixed uniformly, loads in big plastics and tighten bag mouth so that it is continue slowly fermentation 7 days,
Obtain green straw fermented type grass the raising property animal feed of fragrant.
Embodiment three: green corn stalk fermented type spotted deer feed
Step 1, corn stalk steam explosion: choose clean, do not have the corn stalk gone mouldy, and cuts into length 1 centimetre interior
Particle, puts in steam explosion equipment, is passed through high-temperature steam, and reaction condition is controlled in reaction pressure 2.0MPa, reaction temperature
At 110 DEG C after response time 20 min, moment pressure release so that cellulose, hemicellulose in corn stalk come out；
Step 2, straw powder ammonification:
Taking 100 parts of step 1 steam explosion corn stalk powder to load in plastic barrel, it is 22.5% dense for spraying into mass fraction wherein
Ammonia 14 parts, tightens bag mouth, static 48 hours, standby；
Step 3, bovine rumen microorganism species fermentation culture；
In the seed tank that volume is 15L, load anaerobe liquid culture medium 10L, put in microorganism fermentation tank, go out
Bacterium, be cooled to after room temperature inoculate bovine rumen microorganism species Anaerobic culturel original seed 1L, fermentation temperature control 20 DEG C～50 DEG C,
Make microorganism Fast-propagation to viable count 1x10 under the anaerobic environment of pH6.5 ~ 7.010CFU/mL.According to production scale, can be by
According to the ratio of inoculum concentration 10%, bigger fermentation tank expands the cultivation of bovine rumen microorganism species further；
Described bovine rumen microorganism species system inventor butchers to separate rear rumen content from healthy and strong beef cattle and obtains, Qi Zhongzhu
Want microbe species include Ruminococcus albus (Ruminococcusalbus), Butyrivibrio fibrisolvens
(Butyrivibriofibrisolvens), yeast (Saccharomyces), Trichoderma spp. (Trichoderma spp), four kinds are micro-
Bion ratio is about 4:4:1:1；
Described anaerobe liquid culture medium method of completing the square is as follows: seven water disodium hydrogen phosphate 64g, potassium dihydrogen phosphate 15g, chlorine
Changing sodium 2.5g, ammonium chloride 5.0g, microcrystalline Cellulose 10g, the corn stalk powder 20g of beef extract 5g, excessively 200 mesh sieves, constant volume is
1L.Through 121 DEG C of sterilizings, standby；
Step 4, the fermentation opening steps 3 Plastic Drum spiral cover of ammonification corn stalk powder, quickly become to the ammonification corn stalk powder of step 3
Inoculating bovine rumen microorganism species anaerobic cultures in product, inoculum concentration is equivalent to the 10% of ammonification corn stalk grain weight amount；Add simultaneously
Be equivalent to the Sal of powder of straw weight 0.45%, spin lid, roll Plastic Drum and promote content mix homogeneously, lucifuge fermentation 15
My god；
Prepared by step 5, food leaf grass meal: gathering in fresh food leaf grass blade leaf, at 120 DEG C of 5min that complete, 60 DEG C of hot air dryings are to containing
Water rate 8.0%(butt), it is crushed to 40 mesh, standby；
Step 6, green corn stalk fermented type spotted deer feed are allocated: take the corn stalk of step 4 fermenting-ripening under clean environment
70 parts of powder；Food leaf grass meal 10 parts prepared by step 5, is sufficiently mixed uniformly, loads in big plastics and tighten bag mouth so that it is continue slow
Slowly fermenting 5 days, obtains green corn stalk fermented type grass the raising property animal feed of fragrant.
Claims (1)
1. animal feed preparation method raised by a stalk fermentation grass, it is characterised in that include following steps:
Step 1, straw steam explosion:
Choose clean, there is no the straw gone mouldy, cut into the particle that length 1-10 centimetre is interior, put into steam explosion equipment
In, it is passed through high-temperature steam, reaction condition is controlled at reaction pressure 2.0 MPa ~ 3.5MPa, reaction temperature 110 DEG C ~ 130 DEG C
After response time 20 min ~ 40 min, moment pressure release so that cellulose, hemicellulose in straw come out, described straw
Stalk is Caulis et Folium Oryzae, straw, corn stalk；
Step 2, straw ammoniation:
Taking step 1 straw steam explosion stalk powder 100 parts to load in plastic barrel, spraying into mass fraction wherein is 22.5%
Strong aqua ammonia 10 parts ~ 15 parts, tightens bag mouth, static 24-70 hour, standby；
Step 3, bovine rumen microorganism species fermentation culture；
In the seed tank that volume is 15L, load anaerobe liquid culture medium 10L, put in microorganism fermentation tank, go out
Bacterium, be cooled to after room temperature inoculate bovine rumen microorganism species Anaerobic culturel original seed 1L, fermentation temperature control 20 DEG C～50 DEG C,
Make microorganism Fast-propagation to viable count 1x10 under the anaerobic environment of pH6.5 ~ 7.010CFU/mL, according to production scale, can be by
According to the ratio of inoculum concentration 10%, bigger fermentation tank expands the cultivation of bovine rumen microorganism species further；
Described bovine rumen microorganism species obtains for butchering to separate rear rumen content from healthy and strong beef cattle, wherein major microorganisms kind
Class include Ruminococcus albus (Ruminococcusalbus), Butyrivibrio fibrisolvens (Butyrivibriofibrisolvens),
Yeast (Saccharomyces), Trichoderma spp. (Trichoderma spp), four kinds of microorganism individuality ratios are about 4:4:1:1；
Described anaerobe liquid culture medium method of completing the square is as follows: seven water disodium hydrogen phosphate 64g, potassium dihydrogen phosphate 15g, chlorine
Change sodium 2.5g, ammonium chloride 5.0g, microcrystalline Cellulose 10g, beef extract 5g, cross 200 mesh sieves and rice straw powder 20g, constant volume is
1L, through 121 DEG C of sterilizings, standby；
Step 4, the fermentation culture opening steps 3 Plastic Drum spiral cover of ammoniated straw, quickly connect in step 3 ammoniated straw finished product
Planting bovine rumen microorganism species anaerobic cultures, inoculum concentration is equivalent to the 10% ~ 15% of stalk powder weight；Add simultaneously and be equivalent to
The Sal of powder of straw weight 0.5%, spins lid, rolls Plastic Drum and promotes content mix homogeneously, and lucifuge is fermented 15 days ~ 30 days
；
Prepared by step 5, food leaf grass meal: gather in fresh food leaf grass blade leaf, in 120 DEG C of 5min ~ 10min that complete, 60 DEG C ~ 80 DEG C heat
Wind is dried to moisture content 8%～12%(butt), it is crushed to 40～60 mesh, standby；
Animal feed allotment raised by step 6, a kind of stalk fermentation grass: take the powder of straw 70 of step 4 fermenting-ripening under clean environment
Part-90 parts；Food leaf grass meal 10 parts-20 parts prepared by step 5, is sufficiently mixed uniformly, loads in big plastics and tighten bag mouth so that it is
Continuing slowly fermentation 5 days ~ 10 days, animal feed raised by the green stalk fermentation type grass obtaining fragrant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610688627.XA CN106306359A (en) | 2016-08-19 | 2016-08-19 | Method for preparing feed for grass feed animal through straw fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610688627.XA CN106306359A (en) | 2016-08-19 | 2016-08-19 | Method for preparing feed for grass feed animal through straw fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106306359A true CN106306359A (en) | 2017-01-11 |
Family
ID=57743024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610688627.XA Pending CN106306359A (en) | 2016-08-19 | 2016-08-19 | Method for preparing feed for grass feed animal through straw fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106306359A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107198019A (en) * | 2017-05-17 | 2017-09-26 | 安徽康桥生物科技有限公司 | The method that the full-time grain of plant-eating animal is made using agricultural crop straw |
| CN107319148A (en) * | 2017-08-19 | 2017-11-07 | 青岛嘉瑞生物技术有限公司 | A kind of growth promotion ecological immunity type sika deer special feed and preparation method thereof |
| CN107912613A (en) * | 2017-11-29 | 2018-04-17 | 柏绿山 | With the feed and its production method of food leaf grass production |
| CN108850552A (en) * | 2018-07-09 | 2018-11-23 | 康桥投资控股有限公司 | The preparation method of corn straw feedstuff fermentation |
| CN110558438A (en) * | 2019-10-24 | 2019-12-13 | 甘肃省农业科学院畜草与绿色农业研究所(甘肃省农业科学院农业质量标准与检测技术研究所) | Method for preparing silage mixed by ryegrass and whole corn for cattle and sheep |
| CN115176868A (en) * | 2022-07-29 | 2022-10-14 | 安徽粮牧农业科技有限公司 | Straw mixed feed and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816370A (en) * | 2010-03-10 | 2010-09-01 | 南京农业大学 | Gas eruption-based method for preparing feed with high nutritive value by crop straws |
| CN101940248A (en) * | 2009-07-06 | 2011-01-12 | 中国科学院过程工程研究所 | Method for preparing feed by mixing straw and butanol or alcoholic fermentation waste water |
| CN102246913A (en) * | 2011-07-25 | 2011-11-23 | 上海牧农生物科技有限公司 | Biologically fermented straw feed prepared through anaerobic or facultative fermentation |
| CN102586110A (en) * | 2012-02-22 | 2012-07-18 | 无锡丰陆环保科技有限公司 | Facultative anaerobe microbial preparation, its preparation method and application in straw degrading |
-
2016
- 2016-08-19 CN CN201610688627.XA patent/CN106306359A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940248A (en) * | 2009-07-06 | 2011-01-12 | 中国科学院过程工程研究所 | Method for preparing feed by mixing straw and butanol or alcoholic fermentation waste water |
| CN101816370A (en) * | 2010-03-10 | 2010-09-01 | 南京农业大学 | Gas eruption-based method for preparing feed with high nutritive value by crop straws |
| CN102246913A (en) * | 2011-07-25 | 2011-11-23 | 上海牧农生物科技有限公司 | Biologically fermented straw feed prepared through anaerobic or facultative fermentation |
| CN102586110A (en) * | 2012-02-22 | 2012-07-18 | 无锡丰陆环保科技有限公司 | Facultative anaerobe microbial preparation, its preparation method and application in straw degrading |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107198019A (en) * | 2017-05-17 | 2017-09-26 | 安徽康桥生物科技有限公司 | The method that the full-time grain of plant-eating animal is made using agricultural crop straw |
| CN107319148A (en) * | 2017-08-19 | 2017-11-07 | 青岛嘉瑞生物技术有限公司 | A kind of growth promotion ecological immunity type sika deer special feed and preparation method thereof |
| CN107912613A (en) * | 2017-11-29 | 2018-04-17 | 柏绿山 | With the feed and its production method of food leaf grass production |
| CN108850552A (en) * | 2018-07-09 | 2018-11-23 | 康桥投资控股有限公司 | The preparation method of corn straw feedstuff fermentation |
| CN110558438A (en) * | 2019-10-24 | 2019-12-13 | 甘肃省农业科学院畜草与绿色农业研究所(甘肃省农业科学院农业质量标准与检测技术研究所) | Method for preparing silage mixed by ryegrass and whole corn for cattle and sheep |
| CN115176868A (en) * | 2022-07-29 | 2022-10-14 | 安徽粮牧农业科技有限公司 | Straw mixed feed and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106173363B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using edible fungus bran | |
| CN102113623B (en) | Method for producing biological particle grain-saving straw feed | |
| CN106173362A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with Broussonetia papyrifera branch and leaf | |
| CN106306359A (en) | Method for preparing feed for grass feed animal through straw fermentation | |
| CN103859155B (en) | Containing the process for purification of profitable probliotics cassava feed | |
| CN106260541A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with Ramulus Mori | |
| CN104543393A (en) | Processing method of comprehensive utilization of edible-medicinal fungi mushroom bran | |
| CN103283954A (en) | Method for preparing biological feed from sugarcane caudate lobe as main raw material | |
| CN103283955A (en) | Method for preparing biological feed from rice straw | |
| CN106212916B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using sugarcane tail | |
| CN103283956A (en) | Method for preparing biological feed from banana straw, stems and leaves | |
| CN106173365A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with Flos Jasmini Sambac slag | |
| CN103535512A (en) | Preparation method for microbial straw micro-storage feeds | |
| CN106173361A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with herbage | |
| CN106234755B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using bagasse | |
| CN106173364A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with corn straw | |
| CN104987156A (en) | Lyophyllum fumosurn culture medium using fermented bran and method for cultivating lyophyllum fumosurn | |
| CN106212879A (en) | The method producing cattle and sheep complete feed for raw material ferment in second time with Maninot esculenta crantz. stem and leaf | |
| CN104304666A (en) | Method for producing feed by fermenting straw by compound microorganisms | |
| CN101298593B (en) | Straw microorganism composite transformation agent, preparation and use in protein feed | |
| CN104322879A (en) | Preparation method of activated ferment straw biscuit feed | |
| CN102138637A (en) | Formula and preparation process of transforming agent of organic feed of grain-free biomass | |
| CN109105271A (en) | A kind of padding and preparation method thereof of vaccary cowshed | |
| CN102960538B (en) | Unicellular protein feed prepared from fermented dragon fruit peel and production method of unicellular protein feed | |
| CN104855725A (en) | Millet straw leavening technology for mixed granulated feed for ruminants, and mixed granulated feed for ruminants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |