CN104054903A - Production process of fermented cottonseed meal - Google Patents
Production process of fermented cottonseed meal Download PDFInfo
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- CN104054903A CN104054903A CN201410248620.7A CN201410248620A CN104054903A CN 104054903 A CN104054903 A CN 104054903A CN 201410248620 A CN201410248620 A CN 201410248620A CN 104054903 A CN104054903 A CN 104054903A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a production process of fermented cottonseed meal. The process comprises the following steps: breeding lactobacilli, bifidobacteria, bacillus subtilis and candida utilis by adopting a self-made culture medium at the same time, mixing obtained strain liquid, acid protease, cellulase, aspergillus oryzae and yeast enzyme with cottonseed meal, performing fermentation production, drying, grinding and packaging. According to the fermented cottonseed meal produced by adopting the process, free gossypol in the cottonseed meal can be effectively degraded, the palatability of the cottonseed meal can be improved, macromolecular proteins are decomposed into micromolecular polypeptides or amino acids convenient to absorb by animals, crude proteins and crude fibers are degraded, a large quantity of active probiotics are secreted and synthesized, and the nutritional value of the cottonseed meal as a feed is improved.
Description
Technical field
The present invention relates to technical field of biological fermentation, be specifically related to a kind of production technology of the cotton dregs that ferment.
Technical background
Cotton dregs are the face cakes after squeezing cottonseed, through extract technology, most of Residual oil of the inside is separated again, a kind of micro-red or yellow granular article obtaining, it is the primary raw material of Fodder making, and wherein containing crude fibre, thick protein, mineral matter is mainly phosphorus, but many platymisciums phosphorus, utilization rate is low, contain in addition the gossypol that harmfulness is larger, gossypol is mainly present in cotton benevolence pigment gland, is a kind of water insoluble and be dissolved in the poly-phenol pigment of yellowish-brown of organic solvent.In liquefaction process, fry owing to steaming, the heat effects such as squeezing, most of gossypol is combined and is become bound gossoypol with protein, amino acid, and bound gossoypol is not absorbed by animal in animal alimentary canal, and toxicity is very little; Another part gossypol is present in cake, the dregs of rice and oil product with free form, this part free gossypol is larger to animal toxicity, especially nonruminant excess ingestion or picked-up time longer, can cause growth retardation, reproductive performance and production performance to decline, even cause death.Brood is lower to the tolerance of gossypol.
Patent CN102178035A discloses a kind of method that gossypol in cotton dregs is decomposed in composite bacteria fermentation, reduce the content of cotton dregs Free Gossypols, patent CN102805208A discloses a kind of preparation method of high-lysine reduce toxicity cotton dregs, in the content that reduces cotton dregs Free Gossypol, improve lysine content, but how to improve to a greater extent the trap of animal to fermentation cotton dregs, improving to a greater extent its utilization rate as feed and nutritive value is still urgent problem.
Summary of the invention
The present invention seeks to the deficiency for solving the problems of the technologies described above, a kind of production technology of the cotton dregs that ferment is provided, the cotton dregs Free Gossypol of effectively degrading, improves its palatability, degraded crude protein and crude fibre, secretion and synthetic a large amount of active probiotics, improve the nutritive value of cotton dregs as feed.
The present invention for solving the problems of the technologies described above adopted technical scheme is: with making culture medium by oneself, lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili bacterium are expanded to cultivation, after mixing with raw materials such as cotton dregs with gained nutrient solution, acid protease, cellulase, aspergillus oryzae and yeast enzyme, carry out fermenting and producing, and then packaging after drying pulverizing, step is as follows:
(1) preparation of bacterial classification bacterium liquid:
By following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.0-3.0:1.5-1.8:2.0-3.0:0.5-1.0:0.3-0.4:0.015-0.020:0.5-0.8:180-220, be heated to boiling, regulate pH value to proceed in saccharifying tank to 4.5-5.5, in saccharifying tank, add 9 × 10
5u-10 × 10
5the carbohydrase of U, 55-65 DEG C of insulation 3-5 hour, obtains saccharified liquid, and gained saccharified liquid is proceeded in fluid heater and is warming up to 95-100 DEG C, is incubated 0.5 hour, after within 5-10 second, be cooled to 35-45 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, and each chemostat is inoculated a kind of bacterial classification, and bacterial classification inoculum concentration, by the percentage of bacterial classification volume and breeding culture volume, is respectively lactic acid bacteria 3-5%, Bifidobacterium 5-7%, bacillus subtilis 5-7%, candida utili bacterium 6-8%, carries out strain fermentation cultivation after inoculation, the cultivation temperature of four chemostats is 35-45 DEG C, and the time is 60-75 hour, wherein lactic acid bacteria, the chemostat of Bifidobacterium carries out sealing and fermenting cultivation, containing bacillus subtilis, the chemostat of candida utili bacterium carries out aerobic fermentation cultivation, oxygen-supply quantity is 1.7-1.9L/min, the viable bacteria content in chemostat breeding culture medium is detected, when viable bacteria content be 9-11 hundred million/gram time, stop the cultivation of bacterial classification in this chemostat, obtain respectively lactic acid bacterial liquid, Bifidobacterium bacterium liquid, bacillus subtilis bacterium liquid, candida utili bacterium bacterium liquid,
(2) fermenting and producing of cotton dregs:
Take the raw material of following mass percent: cotton dregs 65-70%, lactic acid bacterial liquid 0.5-1.5%, Bifidobacterium bacterium liquid 0.5-1.5%, bacillus subtilis bacterium liquid 0.5-1.5%, candida utili bacterium bacterium liquid 0.5-1.5%, acid protease 0.1-0.3%, cellulase 0.1-0.3%, aspergillus oryzae 0.1-0.3%, yeast enzyme 0.1-0.3%, water 25-30% and breeding culture medium 1-3%, mix, be placed in the airtight anaerobic fermentation of solid in installation for fermenting, fermentation temperature 35-45 DEG C, fermentation time 105-115 hour, forms fermentation cotton dregs;
(3) fermentation cotton dregs dry, pulverize, packaging: fermentation cotton dregs are entered in drying machine and are dried, pulverize with pulverizer, after conveying worm output, pack.
The production technology of preferred a kind of cotton dregs that ferment: by following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.4:1.6:2.4:0.8:0.36:0.019:0.6:200, be heated to boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 9.6 × 10
5the carbohydrase of U, 60 DEG C of insulations 4 hours, obtain saccharified liquid, and gained saccharified liquid is proceeded in fluid heater, within 5-10 second, are warming up to 95-100 DEG C, are incubated 0.5 hour, after in 8 seconds, be cooled to 40 DEG C, obtain breeding culture medium, and add in four chemostats.
The production technology of preferred a kind of cotton dregs that ferment: the inoculum concentration of bacterial classification is by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 4%, Bifidobacterium 6%, bacillus subtilis 6%, candida utili bacterium 7%, cultivation temperature is 40 DEG C, and when viable bacteria content be 1,000,000,000/gram time, stop the cultivation of bacterial classification in this chemostat.
The production technology of preferred a kind of cotton dregs that ferment: the part by weight of cotton dregs fermenting and producing Raw is: cotton dregs 68%, lactic acid bacterial liquid 1%, Bifidobacterium bacterium liquid 1%, bacillus subtilis bacterium liquid 1%, candida utili bacterium bacterium liquid 1%, acid protease 0.2%, cellulase 0.2%, aspergillus oryzae 0.2%, yeast enzyme 0.2%, water 25.2%, breeding culture medium 2%.
The production technology of preferred a kind of cotton dregs that ferment: in the fermenting and producing of cotton dregs, fermentation temperature is 40 DEG C, fermentation time 110 hours.
The further production technology of preferred a kind of cotton dregs that ferment: step is as follows:
(1) preparation of bacterial classification bacterium liquid:
By following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.4:1.6:2.4:0.8:0.36:0.019:0.6:200, be heated to boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 9.6 × 10
5the carbohydrase of U, 60 DEG C insulation 4 hours, obtain saccharified liquid, gained saccharified liquid is proceeded in fluid heater and is warming up to 95-100 DEG C, be incubated 0.5 hour, after in 8 seconds, be cooled to 40 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, each chemostat is inoculated a kind of bacterial classification, bacterial classification inoculum concentration is by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 4%, Bifidobacterium 6%, bacillus subtilis 6%, candida utili bacterium 7%, after inoculation, carry out strain fermentation cultivation, cultivation temperature is 40 DEG C, wherein lactic acid bacteria, the chemostat of Bifidobacterium carries out sealing and fermenting cultivation, containing bacillus subtilis, the chemostat of candida utili bacterium carries out aerobic fermentation cultivation, oxygen-supply quantity is 1.8L/min, viable bacteria content in chemostat breeding culture medium is detected, when viable bacteria content be 1,000,000,000/gram time, stop the cultivation of bacterial classification in this chemostat, obtain respectively lactic acid bacterial liquid, Bifidobacterium bacterium liquid, bacillus subtilis bacterium liquid, candida utili bacterium bacterium liquid,
(2) fermenting and producing of cotton dregs:
Take the raw material of following mass percent: cotton dregs 68%, lactic acid bacterial liquid 1%, Bifidobacterium bacterium liquid 1%, bacillus subtilis bacterium liquid 1%, candida utili bacterium bacterium liquid 1%, acid protease 0.2%, cellulase 0.2%, aspergillus oryzae 0.2%, yeast enzyme 0.2%, water 25.2% and breeding culture medium 2%, mix, be placed in the airtight anaerobic fermentation of solid in installation for fermenting, 40 DEG C of fermentation temperatures, fermentation time 110 hours, forms fermentation cotton dregs;
(3) fermentation cotton dregs dry, pulverize, packaging:
Fermentation cotton dregs are entered in drying machine and dried, pulverize with pulverizer, after conveying worm output, pack.
The present invention has the following advantages compared to existing technology: a kind of breeding culture medium prepared by the present invention can be cultivated four kinds of bacterial classifications simultaneously, be convenient to regulate the technological parameters such as the input, time, temperature of material, effectively ensure the viable bacteria content in bacterial classification bacterium liquid, the operation and the time that have reduced breeding, be easy to realize large-scale production; And breeding culture medium is carried out directly adding chemostat to carry out the fermented and cultured of bacterial classification after sterilization treatment, avoided the pollution of breeding culture medium, simplified fermentation flow process simultaneously; The nutrients mass-energy of adding breeding culture medium in the sweat of cotton dregs promotes bacterial classification further to breed, and has saved fermentation time.
In cotton dregs, contain a large amount of free gossypols, crude protein, crude fibre.Free gossypol is harmful to animal, cause directly nutrition purposes, especially for feeding animals of cotton dregs, and the free gossypol wherein containing and crude fibre can reduce crude protein utilization in animal body, in the present invention, the microorganism of lactic acid bacteria, Bifidobacterium, bacillus subtilis, candida utili bacterium is in fermentation, generation can destroy the enzyme of gossypol tissue, make the molecular chain rupture of gossypol, greatly reduce the content of free gossypol, and the beneficial flora that the cotton dregs after fermentation contain can improve the palatability of feed, and reduce the generation of Animal diseases;
Crude fibre in cotton dregs is resolved into oligosaccharides or monose by cellulase while fermentation, is convenient to animal and absorbs; And the protein after fermentation, except the crude protein that gossypol self contains, also adds the metabolite of mushroom to some extent, as mycoprotein and digestive ferment, so adding of microorganism mushroom improved the protein content of fermentation cotton dregs greatly; Adding of acid protease can make macromolecular protein resolve into micromolecule polypeptide or amino acid, is convenient to animal and absorbs, and improves the utilization rate of fermentation cotton dregs as animal feed;
Aspergillus oryzae can produce multiple biology enzyme in fermentation, mix use with other enzymes such as yeast enzymes, can be by difficult mass degradations absorbing such as the phytic acid in cotton dregs, and the heat that the noxious material that contains in cotton dregs or inhibiting factor is decomposed or be fermented generation by biology enzyme destroys, and improves the nutritive value of fermentation cotton dregs.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in further detail, but do not form any limitation of the invention.
The production technology that the invention provides a kind of cotton dregs that ferment, step is as follows:
(1) preparation of bacterial classification bacterium liquid:
By following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.0-3.0:1.5-1.8:2.0-3.0:0.5-1.0:0.3-0.4:0.015-0.020:0.5-0.8:180-220, be heated to boiling, regulate pH value to proceed in saccharifying tank to 4.5-5.5, in saccharifying tank, add 9 × 10
5u-10 × 10
5the carbohydrase of U, 55-65 DEG C of insulation 3-5 hour, obtains saccharified liquid, and gained saccharified liquid is proceeded in fluid heater and is warming up to 95-100 DEG C, is incubated 0.5 hour, after within 5-10 second, be cooled to 35-45 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, and each chemostat is inoculated a kind of bacterial classification, and bacterial classification inoculum concentration, by the percentage of bacterial classification volume and breeding culture volume, is respectively lactic acid bacteria 3-5%, Bifidobacterium 5-7%, bacillus subtilis 5-7%, candida utili bacterium 6-8%, carries out strain fermentation cultivation after inoculation, the cultivation temperature of four chemostats is 35-45 DEG C, and the time is 60-75 hour, wherein lactic acid bacteria, the chemostat of Bifidobacterium carries out sealing and fermenting cultivation, containing bacillus subtilis, the chemostat of candida utili bacterium carries out aerobic fermentation cultivation, oxygen-supply quantity is 1.7-1.9L/min, the viable bacteria content in chemostat breeding culture medium is detected, when viable bacteria content be 9-11 hundred million/gram time, stop the cultivation of bacterial classification in this chemostat, obtain respectively lactic acid bacterial liquid, Bifidobacterium bacterium liquid, bacillus subtilis bacterium liquid, candida utili bacterium bacterium liquid,
(2) fermenting and producing of cotton dregs:
Take the raw material of following mass percent: cotton dregs 65-70%, lactic acid bacterial liquid 0.5-1.5%, Bifidobacterium bacterium liquid 0.5-1.5%, bacillus subtilis bacterium liquid 0.5-1.5%, candida utili bacterium bacterium liquid 0.5-1.5%, acid protease 0.1-0.3%, cellulase 0.1-0.3%, aspergillus oryzae 0.1-0.3%, yeast enzyme 0.1-0.3%, water 25-30% and breeding culture medium 1-3%, mix, be placed in the airtight anaerobic fermentation of solid in installation for fermenting, fermentation temperature 35-45 DEG C, fermentation time 105-115 hour, forms fermentation cotton dregs;
(3) fermentation cotton dregs dry, pulverize, packaging: fermentation cotton dregs are entered in drying machine and are dried, pulverize with pulverizer, after conveying worm output, pack.
The production technology of preferred a kind of cotton dregs that ferment: by following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.4:1.6:2.4:0.8:0.36:0.019:0.6:200, be heated to boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 9.6 × 10
5the carbohydrase of U, 60 DEG C of insulations 4 hours, obtain saccharified liquid, and gained saccharified liquid is proceeded in fluid heater, within 5-10 second, are warming up to 95-100 DEG C, are incubated 0.5 hour, after in 8 seconds, be cooled to 40 DEG C, obtain breeding culture medium, and add in four chemostats.
The production technology of preferred a kind of cotton dregs that ferment: the inoculum concentration of bacterial classification is by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 4%, Bifidobacterium 6%, bacillus subtilis 6%, candida utili bacterium 7%, cultivation temperature is 40 DEG C, and when viable bacteria content be 1,000,000,000/gram time, stop the cultivation of bacterial classification in this chemostat.
The production technology of preferred a kind of cotton dregs that ferment: the part by weight of cotton dregs fermenting and producing Raw is: cotton dregs 68%, lactic acid bacterial liquid 1%, Bifidobacterium bacterium liquid 1%, bacillus subtilis bacterium liquid 1%, candida utili bacterium bacterium liquid 1%, acid protease 0.2%, cellulase 0.2%, aspergillus oryzae 0.2%, yeast enzyme 0.2%, water 25.2%, breeding culture medium 2%.
The production technology of preferred a kind of cotton dregs that ferment: in the fermenting and producing of cotton dregs, fermentation temperature is 40 DEG C, fermentation time 110 hours.
The further production technology of preferred a kind of cotton dregs that ferment: step is as follows:
(1) preparation of bacterial classification bacterium liquid:
By following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.4:1.6:2.4:0.8:0.36:0.019:0.6:200, be heated to boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 9.6 × 10
5the carbohydrase of U, 60 DEG C insulation 4 hours, obtain saccharified liquid, gained saccharified liquid is proceeded in fluid heater and is warming up to 95-100 DEG C, be incubated 0.5 hour, after in 8 seconds, be cooled to 40 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, each chemostat is inoculated a kind of bacterial classification, bacterial classification inoculum concentration is by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 4%, Bifidobacterium 6%, bacillus subtilis 6%, candida utili bacterium 7%, after inoculation, carry out strain fermentation cultivation, cultivation temperature is 40 DEG C, wherein lactic acid bacteria, the chemostat of Bifidobacterium carries out sealing and fermenting cultivation, containing bacillus subtilis, the chemostat of candida utili bacterium carries out aerobic fermentation cultivation, oxygen-supply quantity is 1.8L/min, viable bacteria content in chemostat breeding culture medium is detected, when viable bacteria content be 1,000,000,000/gram time, stop the cultivation of bacterial classification in this chemostat, obtain respectively lactic acid bacterial liquid, Bifidobacterium bacterium liquid, bacillus subtilis bacterium liquid, candida utili bacterium bacterium liquid,
(2) fermenting and producing of cotton dregs:
Take the raw material of following mass percent: cotton dregs 68%, lactic acid bacterial liquid 1%, Bifidobacterium bacterium liquid 1%, bacillus subtilis bacterium liquid 1%, candida utili bacterium bacterium liquid 1%, acid protease 0.2%, cellulase 0.2%, aspergillus oryzae 0.2%, yeast enzyme 0.2%, water 25.2% and breeding culture medium 2%, mix, be placed in the airtight anaerobic fermentation of solid in installation for fermenting, 40 DEG C of fermentation temperatures, fermentation time 110 hours, forms fermentation cotton dregs;
(3) fermentation cotton dregs dry, pulverize, packaging:
Fermentation cotton dregs are entered in drying machine and dried, pulverize with pulverizer, after conveying worm output, pack.
The present invention is the production technology of preferred a kind of cotton dregs that ferment further, specifically fermentation material proportion and technological parameter, the free gossypol content of cotton dregs of making to ferment is reduced to by fermentation front 8.5%, the content of protein improves 11.62%, coarse-fibred content reduces by 6.17%, and composite bacteria reaches 45,000,000,000/gram.
Embodiment 1
The present embodiment comprises the following steps:
(1) preparation of bacterial classification bacterium liquid:
Fructus hordei germinatus 2.5kg, peptone 1.7kg, agar 2.5kg, natrium citricum 0.8kg, potassium dihydrogen phosphate 0.3kg, magnesium sulfate 0.018kg, sodium acid carbonate 0.7kg and water 190kg are added in material-compound tank, be heated to boiling, after regulating pH value to 4.8, proceed in saccharifying tank, in saccharifying tank, add 10 × 10
5the carbohydrase of U, 65 DEG C insulation 3 hours, obtain saccharified liquid, gained saccharified liquid is proceeded in fluid heater and is warming up to 95-100 DEG C, be incubated 0.5 hour, after in 10 seconds, be cooled to 35 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, each chemostat is inoculated a kind of bacterial classification, the bacterial classification that connects by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 5%, Bifidobacterium 7%, bacillus subtilis 7%, candida utili bacterium 8%, after inoculation, carry out strain fermentation cultivation, wherein the chemostat of lactic acid bacteria and Bifidobacterium carries out sealing and fermenting cultivation, bacillus subtilis, candida utili bacterium carries out aerobic fermentation cultivation, cultivation temperature is 35 DEG C, time is 75 hours, obtaining lactic acid bacterial liquid: viable bacteria content is 9.5 hundred million/gram, Bifidobacterium bacterium liquid: viable bacteria content is 1,000,000,000/gram, bacillus subtilis bacterium liquid: viable bacteria content is 1,000,000,000/gram, candida utili bacterium bacterium liquid: viable bacteria content is 9.5 hundred million/gram,
(2) fermenting and producing of cotton dregs:
Take following raw material: cotton dregs 500kg, lactic acid bacterial liquid 5kg, Bifidobacterium bacterium liquid 5kg, bacillus subtilis bacterium liquid 5kg, candida utili bacterium bacterium liquid 5kg, acid protease 1kg, cellulase 1kg, aspergillus oryzae 1kg, yeast enzyme 1kg, water 200kg and breeding culture medium 10kg, mix, be placed in the airtight anaerobic fermentation of solid in installation for fermenting, 45 DEG C of fermentation temperatures, fermentation time 105 hours, forms fermentation cotton dregs;
(3) fermentation cotton dregs dry, pulverize, packaging: fermentation cotton dregs are entered in drying machine and are dried, pulverize with pulverizer, after conveying worm output, pack.
Embodiment 2
The present embodiment comprises the following steps:
(1) preparation of bacterial classification bacterium liquid:
Fructus hordei germinatus 2.4kg, peptone 1.6kg, agar 2.4kg, natrium citricum 0.8kg, potassium dihydrogen phosphate 0.36kg, magnesium sulfate 0.019kg, sodium acid carbonate 0.6kg and water 200kg are added in material-compound tank, be heated to boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 9.6 × 10
5the carbohydrase of U, 60 DEG C insulation 4 hours, obtain saccharified liquid, gained saccharified liquid is proceeded in fluid heater and is warming up to 95 DEG C, be incubated 0.5 hour, after in 8 seconds, be cooled to 40 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, each chemostat is inoculated a kind of bacterial classification, the bacterial classification that connects by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 4%, Bifidobacterium 6%, bacillus subtilis 6%, candida utili bacterium 7%, after inoculation, carry out strain fermentation cultivation, wherein the chemostat of lactic acid bacteria and Bifidobacterium carries out sealing and fermenting cultivation, bacillus subtilis, candida utili bacterium bacterium carries out aerobic fermentation cultivation, cultivation temperature is 40 DEG C, obtaining lactic acid bacterial liquid: viable bacteria content is 1,000,000,000/gram, Bifidobacterium bacterium liquid: viable bacteria content is 1,000,000,000/gram, bacillus subtilis bacterium liquid: viable bacteria content is 1,000,000,000/gram, candida utili bacterium liquid: viable bacteria content is 1,000,000,000/gram,
(2) fermenting and producing of cotton dregs:
Take following raw material: cotton dregs 680kg, lactic acid bacterial liquid 10kg, Bifidobacterium bacterium liquid 10kg, bacillus subtilis bacterium liquid 10kg, candida utili bacterium bacterium liquid 10kg, acid protease 2kg, cellulase 2kg, aspergillus oryzae 2kg, yeast enzyme 2kg, water 252kg and breeding culture medium 20kg, mix, be placed in the airtight anaerobic fermentation of solid in installation for fermenting, 40 DEG C of fermentation temperatures, fermentation time 110 hours, forms fermentation cotton dregs;
3) be dried, pulverize, pack: fermentation cotton dregs are entered in drying machine and dried, pulverize with pulverizer, after conveying worm output, pack.
Embodiment 3
The present embodiment comprises the following steps:
(1) preparation of bacterial classification bacterium liquid:
Fructus hordei germinatus 3.0kg, peptone 1.5kg, agar 2.0kg, natrium citricum 0.5kg, potassium dihydrogen phosphate 0.4kg, magnesium sulfate 0.020kg, sodium acid carbonate 0.5kg and water 210kg are added in material-compound tank, be heated to boiling, after regulating pH value to 4.5, proceed in saccharifying tank, in saccharifying tank, add 9.4 × 10
5the carbohydrase of U, 55 DEG C insulation 4 hours, obtain saccharified liquid, gained saccharified liquid is proceeded in fluid heater and is warming up to 98 DEG C, be incubated 0.5 hour, after in 7 seconds, be cooled to 45 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, each chemostat is inoculated a kind of bacterial classification, bacterial classification inoculum concentration is by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 3%, Bifidobacterium 5%, bacillus subtilis 5%, candida utili bacterium 6%, after inoculation, carry out strain fermentation cultivation, wherein lactic acid bacteria and Bifidobacterium carry out anaerobic fermentation cultivation, bacillus subtilis, candida utili bacterium carries out aerobic fermentation cultivation, cultivation temperature is 45 DEG C, time is 65 hours, obtaining lactic acid bacterial liquid: viable bacteria content is 10.5 hundred million/gram, Bifidobacterium bacterium liquid: viable bacteria content is 9.4 hundred million/gram, bacillus subtilis bacterium liquid: viable bacteria content is 9.2 hundred million/gram, candida utili bacterium bacterium liquid: viable bacteria content is 9.5 hundred million/gram,
(2) fermenting and producing of cotton dregs:
Take following raw material: cotton dregs 500kg, lactic acid bacterial liquid 8kg, Bifidobacterium bacterium liquid 8kg, bacillus subtilis bacterium liquid 8kg, candida utili bacterium bacterium liquid 8kg, acid protease 1.5kg, cellulase 1.5kg, aspergillus oryzae 1.5kg, yeast enzyme 1.5kg, water 200kg and breeding culture medium 15kg, mix, be placed in the airtight anaerobic fermentation of solid in installation for fermenting, 45 DEG C of fermentation temperatures, fermentation time 105 hours, forms fermentation cotton dregs;
(3) fermentation cotton dregs dry, pulverize, packaging: fermentation cotton dregs are entered in drying machine and are dried, pulverize with pulverizer, after conveying worm output, pack.
Experimental result
Wherein, control experiment is the cotton dregs raw material of not processing by fermentation.
Table 1, the impact of different embodiment on free gossypol, protein, crude fibre, compound bacteria content
| Test item | Embodiment 1 | Embodiment 2 | Embodiment 3 | Control experiment |
| Free gossypol (mg/kg) | 80 | 75 | 82 | 880 |
| Protein (%) | 51.26 | 52.12 | 51.12 | 40.50 |
| Crude fibre (%) | 7.15 | 6.95 | 7.28 | 13.12 |
| Compound bacteria (hundred million/g) | 430 | 450 | 425 | 0 |
Claims (6)
1. the ferment production technology of cotton dregs, is characterized in that: comprise following steps:
(1) preparation of bacterial classification bacterium liquid:
By following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.0-3.0:1.5-1.8:2.0-3.0:0.5-1.0:0.3-0.4:0.015-0.020:0.5-0.8:180-220, be heated to boiling, regulate pH value to proceed in saccharifying tank to 4.5-5.5, in saccharifying tank, add 9 × 10
5u-10 × 10
5the carbohydrase of U, 55-65 DEG C of insulation 3-5 hour, obtains saccharified liquid, and gained saccharified liquid is proceeded in fluid heater and is warming up to 95-100 DEG C, is incubated 0.5 hour, after within 5-10 second, be cooled to 35-45 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, and each chemostat is inoculated a kind of bacterial classification, and bacterial classification inoculum concentration, by the percentage of bacterial classification volume and breeding culture volume, is respectively lactic acid bacteria 3-5%, Bifidobacterium 5-7%, bacillus subtilis 5-7%, candida utili bacterium 6-8%, carries out strain fermentation cultivation after inoculation, the cultivation temperature of four chemostats is 35-45 DEG C, and the time is 60-75 hour, wherein lactic acid bacteria, the chemostat of Bifidobacterium carries out sealing and fermenting cultivation, bacillus subtilis, the chemostat of candida utili bacterium carries out aerobic fermentation cultivation, oxygen-supply quantity is 1.7-1.9L/min, the viable bacteria content in chemostat breeding culture medium is detected, when viable bacteria content be 9-11 hundred million/gram time, stop the cultivation of bacterial classification in this chemostat, obtain respectively lactic acid bacterial liquid, Bifidobacterium bacterium liquid, bacillus subtilis bacterium liquid, candida utili bacterium bacterium liquid,
(2) fermenting and producing of cotton dregs:
Take the raw material of following mass percent: cotton dregs 65-70%, lactic acid bacterial liquid 0.5-1.5%, Bifidobacterium bacterium liquid 0.5-1.5%, bacillus subtilis bacterium liquid 0.5-1.5%, candida utili bacterium bacterium liquid 0.5-1.5%, acid protease 0.1-0.3%, cellulase 0.1-0.3%, aspergillus oryzae 0.1-0.3%, yeast enzyme 0.1-0.3%, water 25-30% and breeding culture medium 1-3%, mix, be placed in and in installation for fermenting, carry out the airtight anaerobic fermentation of solid, fermentation temperature 35-45 DEG C, fermentation time 105-115 hour, forms fermentation cotton dregs;
(3) fermentation cotton dregs dry, pulverize, packaging: fermentation cotton dregs are entered in drying machine and are dried, pulverize with pulverizer, after conveying worm output, pack.
2. the production technology of a kind of cotton dregs that ferment according to claim 1, it is characterized in that: by following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.4:1.6:2.4:0.8:0.36:0.019:0.6:200, be heated to boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 9.6 × 10
5u carbohydrase, 60 DEG C insulation 4 hours, obtain saccharified liquid, gained saccharified liquid is proceeded in fluid heater and is warming up to 95 DEG C, be incubated 0.5 hour, after in 8 seconds, be cooled to 40 DEG C, obtain breeding culture medium, and add in four chemostats.
3. the production technology of a kind of cotton dregs that ferment according to claim 1, it is characterized in that: the inoculum concentration of bacterial classification is by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 4%, Bifidobacterium 6%, bacillus subtilis 6%, candida utili bacterium 7%, cultivation temperature is 40 DEG C, and when viable bacteria content be 1,000,000,000/gram time, stop the cultivation of bacterial classification in this chemostat.
4. the production technology of a kind of cotton dregs that ferment according to claim 1, is characterized in that: the part by weight of cotton dregs fermenting and producing Raw is: cotton dregs 68%, lactic acid bacterial liquid 1%, Bifidobacterium bacterium liquid 1%, bacillus subtilis bacterium liquid 1%, candida utili bacterium bacterium liquid 1%, acid protease 0.2%, cellulase 0.2%, aspergillus oryzae 0.2%, yeast enzyme 0.2%, water 25.2%, breeding culture medium 2%.
5. the production technology of a kind of cotton dregs that ferment according to claim 1, is characterized in that: in the fermenting and producing of cotton dregs, fermentation temperature is 40 DEG C, fermentation time 110 hours.
6. the production technology of a kind of cotton dregs that ferment according to claim 1, is characterized in that: step is as follows:
(1) preparation of bacterial classification bacterium liquid:
By following raw material: fructus hordei germinatus, peptone, agar, natrium citricum, potassium dihydrogen phosphate, magnesium sulfate, sodium acid carbonate and water add in material-compound tank, the mass ratio of described raw material is fructus hordei germinatus: peptone: agar: natrium citricum: potassium dihydrogen phosphate: magnesium sulfate: sodium acid carbonate: water=2.4:1.6:2.4:0.8:0.36:0.019:0.6:200, be heated to boiling, after regulating pH value to 5.0, proceed in saccharifying tank, in saccharifying tank, add 9.6 × 10
5the carbohydrase of U, 60 DEG C insulation 4 hours, obtain saccharified liquid, gained saccharified liquid is proceeded in fluid heater and is warming up to 95 DEG C, be incubated 0.5 hour, after in 8 seconds, be cooled to 40 DEG C, obtain breeding culture medium, and add in four chemostats;
By the good lactic acid bacteria of laboratory cultures, Bifidobacterium, bacillus subtilis and candida utili bacterium bacterial classification access respectively in four chemostats with arrow formula inoculation device, each chemostat is inoculated a kind of bacterial classification, bacterial classification inoculum concentration is by the percentage of bacterial classification volume and breeding culture volume, be respectively lactic acid bacteria 4%, Bifidobacterium 6%, bacillus subtilis 6%, candida utili bacterium 7%, after inoculation, carry out strain fermentation cultivation, cultivation temperature is 40 DEG C, wherein lactic acid bacteria, the chemostat of Bifidobacterium carries out sealing and fermenting cultivation, containing bacillus subtilis, the chemostat of candida utili bacterium carries out aerobic fermentation cultivation, oxygen-supply quantity is 1.8L/min, viable bacteria content in chemostat breeding culture medium is detected, when viable bacteria content be 1,000,000,000/gram time, stop the cultivation of bacterial classification in this chemostat, obtain respectively lactic acid bacterial liquid, Bifidobacterium bacterium liquid, bacillus subtilis bacterium liquid, candida utili bacterium bacterium liquid,
(2) fermenting and producing of cotton dregs:
Take the raw material of following mass percent: cotton dregs 68%, lactic acid bacterial liquid 1%, Bifidobacterium bacterium liquid 1%, bacillus subtilis bacterium liquid 1%, candida utili bacterium liquid 1%, acid protease 0.2%, cellulase 0.2%, aspergillus oryzae 0.2%, yeast enzyme 0.2%, water 25.2% and breeding culture medium 2%, mix, be placed in the airtight anaerobic fermentation of solid in installation for fermenting, 40 DEG C of fermentation temperatures, fermentation time 110 hours, forms fermentation cotton dregs;
(3) fermentation cotton dregs dry, pulverize, packaging:
Fermentation cotton dregs are entered in drying machine and dried, pulverize with pulverizer, after conveying worm output, pack.
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| CN104186957A (en) * | 2014-10-01 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Method for improving forage nutritional value of cottonseed meal through microbial fermentation |
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| CN116649473A (en) * | 2022-02-17 | 2023-08-29 | 新疆阿恰尔牧业有限公司 | Method for preparing cotton dreg bricks by fermenting cotton dreg with microorganisms |
| CN114680230A (en) * | 2022-04-01 | 2022-07-01 | 播恩集团股份有限公司 | Low-gossypol high-nutrition fermented cottonseed meal, preparation method and application |
| CN114680230B (en) * | 2022-04-01 | 2022-10-04 | 播恩集团股份有限公司 | Low-gossypol high-nutrition fermented cottonseed meal, preparation method and application |
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| CN115152892B (en) * | 2022-09-07 | 2022-11-25 | 北京挑战农业科技有限公司 | Method for degrading free gossypol in cottonseed meal, product and application thereof |
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