CN101531985B - High-efficiency ferment for fermenting bean pulp and bean pulp fermentation technology using the ferment - Google Patents

High-efficiency ferment for fermenting bean pulp and bean pulp fermentation technology using the ferment Download PDF

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CN101531985B
CN101531985B CN200910111504XA CN200910111504A CN101531985B CN 101531985 B CN101531985 B CN 101531985B CN 200910111504X A CN200910111504X A CN 200910111504XA CN 200910111504 A CN200910111504 A CN 200910111504A CN 101531985 B CN101531985 B CN 101531985B
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fermentation
bean pulp
beans
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ferment
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CN101531985A (en
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卢英华
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Fujian Longyan Minxiong Biotechnology Co ltd
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XIAMEN LIUWEI BIO-TECHNOLOGY Co Ltd
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    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention relates to a high-efficiency ferment for fermenting bean pulp, belonging to the biologic enzymolysis fermentation technical field. The high-efficiency ferment is formed by mixing bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, lactobacillus casei, saccharomyces cerevisiae and candida utilis. The fermentation technology comprises the following steps: crushing the bean pulp and corn; activating the strains; uniformly mixing the bean pulp and the fermentation liquor using a mixer; packing the mixed bean pulp in fermentation bag; fermenting the packaged bean pulp and processing fermented bean pulp: drying-crushing-detecting-packaging-finished product. The technology is simple and the product quality is stable and the content of the anti-nutrient factor in the bean pulp is eliminated and obviously reduced, therefore the bean pulp is a safe plant protein feeder for infant animal with balanced nutrient, good quality and low price and help for health growth of the infant animal, in replace of animal protein feeder of bone powder, fish powder or the like.

Description

The high-efficiency ferment that a kind of bean pulp fermentation is used and use the technology that this starter carries out bean pulp fermentation
Technical field
The present invention relates to the biological enzymolysis fermentation technical field, particularly a kind of efficient soybean meal leaven and the technology of carrying out the fermentation of beans cypress with this efficient soybean meal leaven.
Background technology
Dregs of beans is the most important vegetable protein of livestock and poultry source, its crude protein content height, and by forming than equilibrated amino acid, but owing to have multiple antinutritional factor in the dregs of beans, mainly contain multiple antinutritional factor (Huisman, Jansman.1990 such as trypsin inhibitor, phytic acid, soybean hemoglutinin, urase, oligose, lipoxidase, soybean antigen albumen (sensitizing factor) and goitrin; Jin Zhengyu builds Tianjin, and 1995; Open the courage peak, Wang Liming, 1999; Wang Chunlin, Chen Xibin, 2000; Zheng Aijuan, Zheng Shuqing, 2002).Because their existence plays restraining effect or becomes stodgy one-tenth to grade with the nutritive substance complexing some digestive ferment in the animal body on the one hand, make the digestibility of dregs of beans and the specific absorption of animal descend; On the other hand intravital some organ of animal is played destruction (Li Sufen, Yang Lijie, 1999), physiology, growth, the health of animal is caused bad influence.Especially in recent years Animal nutrition studies show that, the antigen protein in the dregs of beans has limited growing animal to proteinic effective absorption, has influenced the application of dregs of beans in fodder industry.
The elimination of antinutritional factor and proteinic effectively absorbing are the focuses that people pay close attention to always in the dregs of beans.The dregs of beans deep processing method more comes into one's own in recent years, eliminates the influence of the antinutritional factor in the dregs of beans, promotes the more effectively research of degraded and absorbed of soybean protein, has obtained certain progress.The method of microbial fermentation is handled the focus that dregs of beans becomes present research, show that after deliberation dregs of beans has by fermentation reduced the content of antinutritional factor in the dregs of beans largely, effectively eliminate the antigenicity of soybean protein, help growing of animal and absorbing of enteron aisle.
The characteristics that microbe fermentation method is handled dregs of beans: (1) economy, cost are low, do not need heating or add chemical reagent, and no chemical residual is used safer; (2) to the influence of feed nutrient with destroy lessly, can not cause nutritive value to reduce, and can make that nutritive substance is easier to be absorbed by animal; (3) compare with adding single zymin, can act on simultaneously and remove multiple antinutritional factor; (4) dregs of beans cell walls destruction is more thorough, and the easier release of content helps animal and absorbs nutritive substance more apace; (5) increase the thalline that microorganism lives, helped the balance of microbial population of animal intestinal tract; (6) decomposing soya-bean protein produces multiple little peptide (smallpeptides) in the fermenting process.
Reported among the patent application CN 200610020037.2 that activatory prozyme and withered grass bud pole bacterium, milk-acid bacteria, saccharomycetic individual plant bacterial classification or its combination strengthen the technology of bean pulp fermentation.(the microbial fermentation dregs of beans nutritive property research of forage science institute of Zhejiang University; China's grain and oil journal, 2008,1, Vol.23, p.121-124) research also adopt withered grass bud pole bacterium, S. cervisiae, milk-acid bacteria that dregs of beans is fermented, dregs of beans by fermentation after, removed antinutritional factor in the dregs of beans, the protein content of dregs of beans also is improved, and crude fat, phosphorus, amino acid whose content also all have increase.In the fermenting process, microorganism breeds in a large number, and its result has not only improved the protein level of fermented soybean albumen base-material, and the fermentation of part soybean protein the time is converted into the nutritional quality that tropina itself has also changed soybean shape white matter.Dregs of beans utilization ratio and proteinic specific absorption have been improved.
Qiu Jianhong (the application of microbial fermentation dregs of beans in aquaculture; " feed review, technology version ", 2008 the 10th phases, p.8-11) reported that the yeast with high protein enzyme aspergillus, takadiastase aspergillus, phytase generating aspergillus and 5 strain nutrition complements is that the optimum combination bacterial classification is used for bean pulp fermentation, be that effect is good through facts have proved.
But existing microorganism fermentation process long flow path, link are many, easily pollution microbes, fermentation efficiency are low, and the starter consumption is big, the shortcoming that quality be cannot say for sure.
Summary of the invention
For addressing the above problem, the present invention is by the combined fermentation of many bacterial classifications, provide efficiently a kind of, remove the antinutritional factor in the dregs of beans, produce Semen sojae atricolor small peptide, natural lactic acid, enzyme isoreactivity material, can improve many thalline soybean meal leaven of the utilization ratio and the quality product of dregs of beans, the more important thing is that its technical process is simple, fermentation time is short, reduce living contaminants, save time, cost is low.
The present invention is according to Microbiological Principle, provide a kind of by subtilis, Bacillus licheniformis, Lactobacterium acidophilum, lactobacillus lactis, yeast saccharomyces cerevisiae and Candida utilis totally six kinds of microorganisms form unique many thalline soybean meal leaven, by percentage to the quality wherein:
Lactobacillus acidophilus 8%~15%,
Lactobacillus lactis 5%~11%,
Subtilis 58%~64%,
Yeast saccharomyces cerevisiae 10%~16%,
Candida utilis 1%~13%.
More preferably:
Lactobacillus acidophilus 10%~13%,
Lactobacillus lactis 7%~9%,
Subtilis 60%~62%,
Yeast saccharomyces cerevisiae 12%~14%,
Candida utilis 3%~11%.
Most preferably lactobacillus acidophilus accounts for 12%, and lactobacillus lactis accounts for 8%, and subtilis accounts for 60%, and yeast saccharomyces cerevisiae accounts for 12%, and Candida utilis accounts for 8%.
The present invention also provides with the technology of above-mentioned high-efficiency ferment to bean pulp fermentation, it is characterized in that being made up of the following step:
1, preparation of fermentation liquid
Select airtight container, the activated water of packing into, wherein activate water and use underground water as far as possible and non-tap water, then efficient soybean meal leaven, brown sugar and water are mixed, wherein the ratio of starter, brown sugar, activated water is 1: 4~12: 140~200, leave standstill more than 60 minutes, starter is fully activated, standby.Fermented liquid left standstill at 25~40 ℃ following re-use after cultivating 8~12 hours, the fermenting speed of dregs of beans can be faster.
2, the pulverizing of dregs of beans
The dregs of beans raw material sources are in grain, oil food processing enterprise, high quality raw material will meet the requirement of GB/T 19541-2004, also can select for use general dregs of beans to make raw material, but to the dregs of beans raw material of different manufacturers, different batches, all to carry out strict check, so that select suitable enzymatic hydrolysis and fermentation processing parameter.
For dregs of beans is contacted fully with bacterial classification, make fermentation more thorough, also need before the fermentation dregs of beans is carried out pulverization process, suitably add corn during pulverizing, can strengthen the effect of fermentation, the addition of corn is 4~9kg/ ton dregs of beans.The fineness of pulverizing is controlled at about 40 orders is advisable.The consumption of efficient soybean meal leaven is the efficient soybean meal leaven of 1~1.5Kg/ton dregs of beans, the efficient soybean meal leaven of preferred 1~1.3Kg/ton dregs of beans.
3, mix
The dregs of beans and the fermented liquid that crush are mixed with mixing machine, and correct operating methods is in the mixing process: fermented liquid evenly is sprayed onto in the dregs of beans lentamente, and spray on the limit, the limit mixes.
4. fermentation
With the bean pulp fermentation pack that mixes, ferment.Temperature indoor when the length of fermentation time and fermentation is closely related, and the optimum temperuture of fermentation is 28~40 ℃, and is very fast at 40 ℃ of left and right sides fermenting speeds, in the environment more than 28 ℃, only needs 2~3 days time just can finish fermentation.
Generally after pack 14 hours, the fermentation bag inner bag begins flatulence, and give out the fragrant smell of acid, along with thickening of sour fragrance, the process that a flatulence is cooled down again will take place in the vacant position of fermentation bag, and the flatulence process is very fast, and the process of cooling down is relatively slow, treat the state when sack returns to pack substantially, fermentation is finished.Flatulence is relevant with process of cooling down and temperature, and process was short more obvious more when temperature was high more, otherwise then long more not obvious more
5, fermentation post-treatment
Described fermentation post-treatment is meant the technology of fermentation aftertreatment: oven dry-pulverizing-check-packing-finished product.
Moist fermented bean dregs is dried processing, and drying mode has cylinder oven dry, fluidized-bed oven dry, three kinds of modes of very low temperature spraying drying basically.Drying requirement is to be 1~2 hour the time, and moisture is below 1 0%.Pulverize (being crushed to 80 orders) after the oven dry again by 40 orders.The specific targets that detect and pack meet company standard.
The present invention also provides another with the technology of above-mentioned high-efficiency ferment to bean pulp fermentation, it is characterized in that:
With high-efficiency ferment provided by the present invention and water by after 1: 9 the dilution proportion, then with fermented liquid 30 ℃ following leave standstill cultivate 8 hours after, directly mix with the dregs of beans dry powder that crushes.This process does not need the brown sugar activation.Fermentation remains between 20~28 ℃, and the time that fermentation is finished was at 3~4 days.The consumption of efficient soybean meal leaven is the efficient soybean meal leaven of 1~1.5Kg/ton dregs of beans, the efficient soybean meal leaven of preferred 1~1.3Kg/ton dregs of beans.Described water is from underground water or the water that dechlorinated.
The present invention has the following advantages:
(1) every kind of microorganism is optimized screening and domestication, makes it to have acidproof, salt tolerant, heatproof and anti-biliary good characteristic.
(2) high-efficiency ferment fermentation efficiency height of the present invention, its consumption 1~1.5Kg/ ton dregs of beans, more existing starter has significantly reduced consumption, has reduced cost.
(2) fermentation pattern and process innovation have reduced fermentation step, shorten fermentation period, reduce facility investment, have cost advantage.
(3) application innovation can directly be used the not fermented bean dregs of oven dry in plant, reduces cost, strengthens product function.
Embodiment
High-efficiency ferment provided by the invention is carried out being described in further detail of technology of bean pulp fermentation below in conjunction with specific embodiment:
Embodiment 1
When lactobacillus acidophilus accounts for 12%, lactobacillus lactis accounts for 8%, and subtilis accounts for 60%, and yeast saccharomyces cerevisiae accounts for 12%, and Candida utilis accounted for 8% o'clock, made efficient soybean meal leaven.Select airtight container, the underground water about 90kg of packing into mixes efficient soybean meal leaven 500g, brown sugar 3kg and water then, with fermented liquid 40 ℃ following leave standstill cultivate 8 hours after, standby.410kg dregs of beans and 3kg corn are pulverized together, and the fineness of pulverizing is controlled at about 40 orders and is advisable.Fermented liquid evenly is sprayed onto in the dregs of beans that crushes lentamente, and spray on the limit, the limit mixes with mixing machine.With the bean pulp fermentation pack that mixes, 40 ℃ of leavening temperatures packed after 14 hours, and the fermentation bag inner bag begins flatulence, and give out the fragrant smell of acid, along with thickening of sour fragrance, the process that a flatulence is cooled down again will take place in the vacant position of fermentation bag, and the flatulence process is very fast, the process of cooling down is relatively slow, after 48 hours, the state when sack returns to pack substantially, fermentation is finished.The very low temperature spraying drying is crushed to 80 orders by 40 orders then, check, packing.
Embodiment 2
When lactobacillus acidophilus accounts for 11%, lactobacillus lactis accounts for 9%, and subtilis accounts for 60%, and yeast saccharomyces cerevisiae accounts for 14%, and Candida utilis accounted for 6% o'clock, made efficient soybean meal leaven.Select airtight container, the underground water about 100kg of packing into mixes efficient soybean meal leaven 500g, brown sugar 3kg and water then, with fermented liquid 37 ℃ following leave standstill cultivate 9 hours after, standby.400kg dregs of beans and 3.5kg corn are pulverized together, and the fineness of pulverizing is controlled at about 40 orders and is advisable.Fermented liquid evenly is sprayed onto in the dregs of beans that crushes lentamente, and spray on the limit, the limit mixes with mixing machine.With the bean pulp fermentation pack that mixes, 37 ℃ of leavening temperatures packed after 14 hours, and the fermentation bag inner bag begins flatulence, and give out the fragrant smell of acid, along with thickening of sour fragrance, the process that a flatulence is cooled down again will take place in the vacant position of fermentation bag, and the flatulence process is very fast, the process of cooling down is relatively slow, after 56 hours, the state when sack returns to pack substantially, fermentation is finished.The cylinder bake drying is crushed to 80 orders by 40 orders then, check, packing.
Embodiment 3
When lactobacillus acidophilus accounts for 13%, lactobacillus lactis accounts for 8%, and subtilis accounts for 60%, and yeast saccharomyces cerevisiae accounts for 12%, and Candida utilis accounted for 7% o'clock, made efficient soybean meal leaven.Select airtight container, the underground water about 70kg of packing into mixes efficient soybean meal leaven 500g, brown sugar 2kg and water then, with fermented liquid 25 ℃ following leave standstill cultivate 12 hours after, standby.500kg dregs of beans and 3.5kg corn are pulverized together, and the fineness of pulverizing is controlled at about 40 orders and is advisable.Fermented liquid evenly is sprayed onto in the dregs of beans that crushes lentamente, and spray on the limit, the limit mixes with mixing machine.With the bean pulp fermentation pack that mixes, 28 ℃ of leavening temperatures packed after 17 hours, and the fermentation bag inner bag begins flatulence, and give out the fragrant smell of acid, along with thickening of sour fragrance, the process that a flatulence is cooled down again will take place in the vacant position of fermentation bag, and the flatulence process is very fast, the process of cooling down is relatively slow, after 72 hours, the state when sack returns to pack substantially, fermentation is finished.The fluidized-bed bake drying is crushed to 80 orders by 40 orders then, check, packing.
Embodiment 4
When lactobacillus acidophilus accounts for 10%, lactobacillus lactis accounts for 7%, and subtilis accounts for 60%, and yeast saccharomyces cerevisiae accounts for 12%, and Candida utilis accounted for 11% o'clock, made efficient soybean meal leaven.Select airtight container, the underground water about 80kg of packing into mixes efficient soybean meal leaven 500g, brown sugar 2kg and water then, with fermented liquid 30 ℃ following leave standstill cultivate 8~10 hours after, standby.420kg dregs of beans and 2.5kg corn are pulverized together, and the fineness of pulverizing is controlled at about 40 orders and is advisable.Fermented liquid evenly is sprayed onto in the dregs of beans that crushes lentamente, and spray on the limit, the limit mixes with mixing machine.With the bean pulp fermentation pack that mixes, 35 ℃ of leavening temperatures packed after 16 hours, and the fermentation bag inner bag begins flatulence, and give out the fragrant smell of acid, along with thickening of sour fragrance, the process that a flatulence is cooled down again will take place in the vacant position of fermentation bag, and the flatulence process is very fast, the process of cooling down is relatively slow, after 48 hours, the state when sack returns to pack substantially, fermentation is finished.The very low temperature spraying drying is crushed to 80 orders by 40 orders then, check, packing.
Embodiment 5
When lactobacillus acidophilus accounts for 13%, lactobacillus lactis accounts for 9%, and subtilis accounts for 62%, and yeast saccharomyces cerevisiae accounts for 13%, and Candida utilis accounted for 3% o'clock, made efficient soybean meal leaven.Select airtight container, the underground water about 90kg of packing into mixes efficient soybean meal leaven 500g, brown sugar 3kg and water then, with fermented liquid 35 ℃ following leave standstill cultivate 8~10 hours after, standby.410kg dregs of beans and 2.5kg corn are pulverized together, and the fineness of pulverizing is controlled at about 40 orders and is advisable.Fermented liquid evenly is sprayed onto in the dregs of beans that crushes lentamente, and spray on the limit, the limit mixes with mixing machine.With the bean pulp fermentation pack that mixes, 35 ℃ of leavening temperatures packed after 18 hours, and the fermentation bag inner bag begins flatulence, and give out the fragrant smell of acid, along with thickening of sour fragrance, the process that a flatulence is cooled down again will take place in the vacant position of fermentation bag, and the flatulence process is very fast, the process of cooling down is relatively slow, after 48 hours, the state when sack returns to pack substantially, fermentation is finished.The very low temperature spraying drying is crushed to 80 orders by 40 orders then, check, packing.
Embodiment 6
When lactobacillus acidophilus accounts for 12%, lactobacillus lactis accounts for 8%, and subtilis accounts for 60%, and yeast saccharomyces cerevisiae accounts for 12%, and Candida utilis accounted for 8% o'clock, made efficient soybean meal leaven.Select a container with soybean meal leaven 0.5kg, the water 4.5kg that has dechlorinated mixes.Then with fermented liquid 30 ℃ following leave standstill cultivate 8 hours after.The 410kg dregs of beans is pulverized, and the fineness of pulverizing is controlled at about 40 orders and is advisable.Fermented liquid evenly is sprayed onto in the dregs of beans that crushes lentamente, and spray on the limit, the limit mixes with mixing machine.With the bean pulp fermentation pack that mixes, 28 ℃ of leavening temperatures packed after 16 hours, and the fermentation bag inner bag begins flatulence, and give out the fragrant smell of acid, along with thickening of sour fragrance, the process that a flatulence is cooled down again will take place in the vacant position of fermentation bag, and the flatulence process is very fast, the process of cooling down is relatively slow, after 72 hours, the state when sack returns to pack substantially, fermentation is finished.The very low temperature spraying drying is crushed to 80 orders by 40 orders then, check, packing.
Embodiment 7
When lactobacillus acidophilus accounts for 10%, lactobacillus lactis accounts for 9%, and subtilis accounts for 60%, and yeast saccharomyces cerevisiae accounts for 13%, and Candida utilis accounted for 8% o'clock, made efficient soybean meal leaven.Select airtight container, the underground water about 4.5kg of packing into mixes efficient soybean meal leaven 500g then.The 410kg dregs of beans is pulverized, and the fineness of pulverizing is controlled at about 40 orders and is advisable.Fermented liquid evenly is sprayed onto in the dregs of beans that crushes lentamente, and spray on the limit, the limit mixes with mixing machine.With the bean pulp fermentation pack that mixes, 37 ℃ of leavening temperatures packed after 14 hours, and the fermentation bag inner bag begins flatulence, and give out the fragrant smell of acid, along with thickening of sour fragrance, the process that a flatulence is cooled down again will take place in the vacant position of fermentation bag, and the flatulence process is very fast, the process of cooling down is relatively slow, after 60 hours, the state when sack returns to pack substantially, fermentation is finished.The cylinder bake drying is crushed to 80 orders by 40 orders then, check, packing.

Claims (8)

1. the high-efficiency ferment that bean pulp fermentation is used is characterized in that described high-efficiency ferment is mixed by subtilis, Lactobacterium acidophilum, lactobacillus lactis, yeast saccharomyces cerevisiae and Candida utilis, and each component is respectively with the mass percent meter:
Lactobacillus acidophilus 10%~13%,
Lactobacillus lactis 7%~9%,
Subtilis 60%~62%,
Yeast saccharomyces cerevisiae 12%~14%,
Candida utilis 3%~11%.
2. the high-efficiency ferment used of the described bean pulp fermentation of claim 1, it is characterized in that described high-efficiency ferment is mixed by subtilis, Lactobacterium acidophilum, lactobacillus lactis, yeast saccharomyces cerevisiae and Candida utilis, each component is respectively with the mass percent meter:
Lactobacillus acidophilus accounts for 12%, and lactobacillus lactis accounts for 8%, and subtilis accounts for 60%, and yeast saccharomyces cerevisiae accounts for 12%, and Candida utilis accounts for 8%.
3. one kind is carried out the technology of bean pulp fermentation with the described high-efficiency ferment of claim 1, and it comprises the following steps:
1) pulverizing of dregs of beans: dregs of beans and corn are pulverized, and the addition of corn is 4~9kg/ ton dregs of beans, and the fineness of pulverizing is controlled at about 40 orders;
2) actication of culture: select airtight container, the activated water of packing into, then the described efficient soybean meal leaven of claim 1, brown sugar and water are mixed, wherein the ratio of starter, brown sugar, activated water is 1: 4~12: 140~200, fermented liquid is left standstill cultivation 8~12 hours under 25~40 ℃, the consumption of efficient soybean meal leaven is 1~1.5Kg/ ton dregs of beans;
3) mix: the dregs of beans and the fermented liquid that crush are mixed with mixing machine;
4) fermentation: with the bean pulp fermentation pack that mixes, ferment, temperature indoor when the length of fermentation time and fermentation is closely related, and the optimum temperuture of fermentation is 28~40 ℃, only needs 2~3 days time to finish fermentation;
5) fermentation post-treatment: oven dry-pulverizing-check-packing-finished product.
4. claim 3 is described carries out the technology of bean pulp fermentation with high-efficiency ferment, it is characterized in that the preferred activation temperature of microbial strains is 30~37 ℃, preferred soak time 8~9 hours.
5. claim 3 is described carries out the technology of bean pulp fermentation with high-efficiency ferment, and the consumption that it is characterized in that efficient soybean meal leaven is 1~1.3Kg/ ton dregs of beans.
6. claim 3 is described carries out the technology of bean pulp fermentation with high-efficiency ferment, it is characterized in that preferred leavening temperature is 40 ℃, the fermentation time of preferred whole zymotechnique 48~60 hours.
7. one kind is carried out the technology of bean pulp fermentation with the described high-efficiency ferment of claim 1, and it comprises the following steps:
With the described high-efficiency ferment of claim 1 and water by after 1: 9 the dilution proportion, then fermented liquid was left standstill cultivation after 8 hours under 30 ℃, directly mix with the dregs of beans dry powder that crushes, leavening temperature remains between 20~28 ℃, the time that fermentation is finished was at 3~4 days, the consumption of efficient soybean meal leaven is 1~1.5Kg/ ton dregs of beans, and described water is from underground water or the water that dechlorinated.
8. the described high-efficiency ferment of claim 7 carries out the technology of bean pulp fermentation, it is characterized in that: the consumption of efficient soybean meal leaven is 1~1.3Kg/ ton dregs of beans.
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CN107683972B (en) * 2017-10-09 2018-07-31 浙江大学 Improve the fermented feed and its preparation method and application of milking sow production performance
FR3077468B1 (en) * 2018-02-02 2021-12-17 Pascal Philibert LEVAIN AND ITS PACKAGING PROCESS
CN108477407A (en) * 2018-03-08 2018-09-04 山东大学 A kind of compound micro-ecological preparation and its application process in pig-breeding
CN109043142A (en) * 2018-07-19 2018-12-21 中国农业科学院麻类研究所 The fermented bean dregs and its preparation method and application of low pectin content
CN109452450A (en) * 2018-10-18 2019-03-12 桂林精成生物科技有限公司 A kind of industrialized production fermented bean dregs and preparation method thereof
CN109730191A (en) * 2019-01-26 2019-05-10 中国水产科学研究院南海水产研究所 Comprehensive multi-index weighted analysis method optimizes solid state fermentation dregs of beans method
CN112841401A (en) * 2021-02-05 2021-05-28 甘小峰 Production process of compound microbial fermented livestock and poultry green feed
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