CN102972654B - Preparation method of microbial fermented feed without antibiotic for laying hen - Google Patents
Preparation method of microbial fermented feed without antibiotic for laying hen Download PDFInfo
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Abstract
The invention provides a preparation method of a microbial fermented feed without antibiotic for a laying hen, and belongs to the field of the microbial fermented feed. The technical scheme adopted by the preparation method comprises the steps of adding revived fermentation liquid into basic ration of the laying hen to carry out sealed fermentation, wherein the basic ration of the laying hen contains primary ingredients such as corn powder, soya bean meal, rapeseed meal, mulberry leaf powder, salt, edible oil, egg amino, calcium hydrophosphate and mountain flour; drug or chemical additive which is harmful to a human body and animals is not added; fermentation residues and the fermentation liquid are filtered and separated when the material to be fermented emits slightly sour flavor, and the fermentation residues can be directly fed to the animals. The preparation method is simple in preparation process, low in facility request, and easy to promote; the feed does not contain hormone or drug residue, and is safe and environment-friendly, and good in palatability; the nutrition is easily absorbed; the dosage of the feed can be reduced; the cultivation cost is reduced; the egg producing period is prolonged; the egg yield is improved; the cholesterol content of the egg is reduced; the cultivation environment is improved, and the incidence rate is reduced.
Description
Technical field
The present invention relates to a kind of feed, particularly a kind of preparation method of laying hen microbial fermentation antibiotic-free feed, belongs to fermentative feedstuff of microbe field.
Background technology
More and more be subject to people's attention in the raising of nowadays quality of life, high-quality, environmental protection and green and natural grocery trade are more subject to the favor of people.Egg is a main farm produce of China, the modal non-staple foodstuff kind of Ye Shi China resident, and along with improving constantly of living standard, people are while raising egg consumption figure, also more and more higher to the quality requirements of egg.Now China's cultivation still relies on antibiotic present situation and cannot meet the demand of people to healthy food, and along with antibiotic abuse, cause antibody-resistant bacterium constantly to increase, the antibiotic serious threat such as residual to the problem of human health, caused everybody concern.
By adding useful living microorganism in animal feed, there is effect that is supplementary, that regulate, maintain microbial balance in enteron aisle, utilize with the principle of bacterium bacterium, reach minimizing or need not antibiotic object.
Chinese patent (application number: 200810123515.5, denomination of invention: lactobacillus feedstuff for eggs) disclose a kind of lactobacillus feedstuff for eggs, include chicken feed, lactic acid bacteria and shrubby sophora extract, hoary hair's extract, the Herb Gynostemmae Pentaphylli extract of its weight 0.1% ~ 1% is added in chicken feed, it can regulate stomach normal flora, maintain microecological balance, thus improves function of intestinal canal; Improve digestibility and the biological value of food; Reduce serum cholesterol, control endotoxin; Suppress corrupt bacteria growing in enteron aisle; Improve immunity of organisms.Have nontoxic, without the resistance to the action of a drug, noresidue, the feature such as have no side effect.But because it adds single kind of bacterium, action effect is unstable.
Summary of the invention
The present invention adopts biotechnology principle, utilize beneficial microbe to decompose, characteristic that the carbon source utilized in daily ration, nitrogenous source are converted into microbial bacteria body protein, and the advantage of fragrant, the good palatability of feed after fermentation, chicken appetite can be stimulated, improve efficiency of feed utilization, and improve micro-ecological environment in chicken, suppress the growth of pathogenic bacteria in body, improve physique and resistance against diseases, reach the object completely abolished antibiotics medicine and add, a kind of preparation method of laying hen microbial fermentation antibiotic-free feed is provided.The method comprises the following steps:
1. recover: microbial fermentation inoculum is pressed laying hens essential ration percentage by weight 0.3 ~ 0.8% and drop in recovery container, add the warm water of 25 ~ 30 DEG C, amount of water is 2 ~ 4 times of described microbial fermentation inoculum weight, closes and leaves standstill 8 ~ 12h, form recovery bacterium liquid;
Described recovery container is the closed container of adding a cover.
2. ferment: the bacterium liquid of above-mentioned steps 1 having been recovered is diluted to the bacterium liquid dilution of laying hens essential ration gross weight 30%, drop in Zymolysis Equipment with laying hens essential ration, fully stir, close fermentation 3 ~ 24h; Fermentation time is fixed with room temperature, and the time in summer is slightly short, the proper extension time in winter, best results during fermentation materials appearance micro-sour fragrance; For can fully ferment, need timing agitation during the fermentation, be spaced apart stirring per hour 10 minutes;
3. filter: occur after micro-sour fragrance until fermentation materials, by screen pack separation and fermentation liquid and fermentation residue, fermentation residue can direct feeding.
Described microbial fermentation inoculum is the feed fermenting composite bacteria agent capable that Guangzhou Nongguan Biotech Co., Ltd. develops, and its proterties is pulvis, total viable count>=1.0 × 10
9cfu/g, containing useful fungal components such as lactic acid bacteria, saccharomycete, fatty bacterium, bacillus, peptide bacillus.
Guangzhou Nongguan Biotech Co., Ltd. is global transnational enterprise, is to introduce the advanced biotechnology in Taiwan, is devoted to biologic product research and development, produces and sells the new high-tech enterprise be integrated.Company's main management brand has " agriculture hat ", " agriculture is transported ", " garlands " three kinds, principal products of business have microorganism formulation raw material, microorganism purification of water quality, bio-feritlizer function bacterium, feed to add biologic product, organic fertilizer fermentation agent etc., and it is qualified that product quality detects through national authority mechanism.The specifying information of company is understood by network address http://baike.baidu.com/view/5049834.htm.
The composite bacteria agent capable of the said firm's research and development is the demands according to China's feedstuff industry and livestock breeding industry, the probiotics of latest generation on the market of employing international advanced technology and device fabrication, it has the advantages such as breeding is quick, vitality is strong, safety non-toxic, fast decoupled are organic, and animal intestinal thalline can be regulated to balance, improve intestine microenvironment, effective maintenance animal body health status, improving its immunity, resistance against diseases, anti-stress ability, is a kind of animal feed fermenting agent efficiently.
Described laying hens essential ration does not add medicine to human body and animal pest and chemical addition agent, the parts by weight of its key component are: corn flour 580 ~ 700 parts, bean cake powder 120 ~ 200 parts, 120 ~ 200 parts, rapeseed dregs powder, mulberry leaf powder 30 ~ 50 parts, salt 2 ~ 5 parts, edible oil 3 ~ 6 parts, egg amino 0.2 ~ 1 part, calcium monohydrogen phosphate 5 ~ 15 parts, stone flour 2 ~ 8 parts, raw material all can buy in market.Described stone flour is calcium carbonate stone powder.
Mulberry leaf nutrient enriches, be described as in " natural nutrition storehouse ", its crude protein content reaches about 20%, also be rich in trace element and vitamin E and vitamin C, B1, B2, A and the letones such as potassium, calcium, copper, zinc, boron, manganese, mulberry leaf powder is added in laying hen basis solar eclipse, laying hen egg yield, egg size and yolk color can be improved, significantly reduce eggshell crack rate, increase total amino acid content and vitamin content.Guangxi is positioned at Southwestern China portion, has the advantageous condition of development Sericultural production, adds the support of national policy, the province of silkworm and mulberry sparetime university that Xian Yishi China is largest, for the invention process provides abundant raw material resources.
Compared with prior art, the invention has the beneficial effects as follows: manufacture craft of the present invention is simple, low for equipment requirements, very easily promotes; Feed without hormone, medicament residue, safety and environmental protection, good palatability, nutrition easily absorbs, and can reduce feed consumption, reduce aquaculture cost; Extend laying period, improve egg production, reduce the cholesterol level of egg ovum; Improve breeding environment, reduce a sick rate.
Detailed description of the invention
Illustrate below in conjunction with specific embodiment.
one, the preparation method of laying hen microbial fermentation antibiotic-free feed
embodiment 1
1. recover: take the feed fermenting composite bacteria agent capable 5kg that Guangzhou Nongguan Biotech Co., Ltd. develops, drop in recovery container, add 25 ~ 30 DEG C of warm water, amount of water 15Kg, fully stir rear enclosed and leave standstill 10h, form recovery bacterium liquid;
2. ferment: the bacterium liquid of above-mentioned steps 1 having been recovered is diluted to the bacterium liquid dilution of 300kg, drop in Zymolysis Equipment with 1000Kg laying hens essential ration, abundant stirring rear enclosed fermentation, every 1h timing agitation 10min in sweat, so that material fermentation is abundant, until micro-sour fragrance appears in fermentation materials, stop fermentation; Fermentation duration apparent temperature change and adjusting, summer is slightly short, and winter is slightly long, and fermentation time 3 ~ 24h not etc., is not good with feed fermentation to micro-sour fragrance.
3. filter: occur after micro-sour fragrance until fermentation materials, by screen pack separation and fermentation liquid and fermentation residue, fermentation residue can direct feeding.
Wherein, laying hens essential ration does not add medicine to human body and animal pest and chemical addition agent, the parts by weight of its key component are: amino 0.5 part of corn flour 618 parts, bean cake powder 160 parts, 160 parts, rapeseed dregs powder, mulberry leaf powder 40 parts, salt 3 parts, edible oil 5 parts, egg, calcium monohydrogen phosphate 10 parts, stone flour 3.5 parts, raw material is all bought from market.Described stone flour is calcium carbonate stone powder.
two, application test
The feed using above-described embodiment 1 to make below carries out layer breeding contrast test with the antibiotic feed of interpolation.Selective body heavy phase is near, the laying hen 100 of 300 ages in days, be divided into experimental group and control group at random, experimental group is fed the feed that embodiment 1 makes, and control group is only fed former basal diet (containing antibiotic), carries out the contrast tests such as the laying rate of 30 days, feedstuff-egg ratio and rate of broken eggs.Experiment periods adopts and raises in cages, and selects every day in the morning 6 and order feeding in the afternoon 3, ensures chicken free choice feeding.Its experimental group microbial inoculum recovery and fermenting raw materials arrangement of time as follows:
1,1:00 point microbial inoculum recovery in evening, 11:00 point spice ferments, and afternoon, 15:00 point was fed.
2,11:00 point microbial inoculum recovery in the morning, 21:00 point spice ferments, and the next morning, 6:00 point was fed.
In process of the test, every day, 17:00 carried out the data statistics of egg number, inlet amount, broken egg number.Through the test of 30 days by a definite date, result statistics was as following table:
| Index | Control group | Experimental group |
| Chicken number (30d) | 1500 | 1500 |
| Egg number (piece) | 1255 | 1371 |
| Average egg production (%) | 83.67 | 92.73 |
| Average egg kind (g/ piece) | 64.12 | 66.75 |
| Total egg size (Kg) | 80.47 | 91.51 |
| Total feed intake (Kg) | 173.89 | 179.07 |
| Feedstuff-egg ratio | 2.16 | 1.96 |
| Broken egg number (piece) | 9 | 6 |
| Rate of broken eggs (%) | 0.72 | 0.44 |
Result of the test shows: the laying rate of experimental group improves 9.77% than control group, and average egg weight improves 3.94%, and feedstuff-egg ratio reduces by 9.26, and rate of broken eggs reduces by 38.89%, remarkable in economical benefits; In test feeding process, the environment of experimental group is better than control group, and laying period is extended, and the cholesterol level of egg reduces, and has clear superiority compared with normal diet.
Above-mentioned case study on implementation only illustrates specific embodiments of the present invention, and limit absolutely not its actual range, protection scope of the present invention is as the criterion with claims.
Claims (2)
1. the preparation method of laying hen microbial fermentation antibiotic-free feed, is characterized in that, it comprises the following steps:
(1) recover: microbial fermentation inoculum is pressed laying hens essential ration percentage by weight 0.3 ~ 0.8% and drop in recovery container, add the warm water of 25 ~ 30 DEG C, amount of water is 2 ~ 4 times of described microbial fermentation inoculum weight, closes and leaves standstill 8 ~ 12h, forms recovery bacterium liquid;
(2) ferment: the bacterium liquid dilution bacterium liquid that above-mentioned steps (1) has been recovered being diluted to laying hens essential ration gross weight 30%, drop in Zymolysis Equipment with laying hens essential ration, fully stir, close fermentation 3 ~ 24h;
(3) filter: occur after micro-sour fragrance until fermentation materials, by screen pack separation and fermentation liquid and fermentation residue, fermentation residue can direct feeding;
Described microbial fermentation inoculum is the composite bacteria agent capable that Guangzhou Nongguan Biotech Co., Ltd. develops;
Described laying hens essential ration does not add medicine to human body and animal pest and chemical addition agent, and the parts by weight of its key component are: corn flour 580 ~ 700 parts, bean cake powder 120 ~ 200 parts, 120 ~ 200 parts, rapeseed dregs powder, mulberry leaf powder 30 ~ 50 parts, salt 2 ~ 5 parts, edible oil 3 ~ 6 parts, methionine 0.2 ~ 1 part, calcium monohydrogen phosphate 5 ~ 15 parts, stone flour 2 ~ 8 parts;
Described stone flour is calcium carbonate stone powder.
2. the preparation method of laying hen microbial fermentation antibiotic-free feed according to claim 1, is characterized in that needing timing agitation in closed sweat.
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| CN103798544A (en) * | 2014-02-19 | 2014-05-21 | 丹阳市云阳镇田园圣树专业合作社 | Herbal laying hen feed containing mulberry branches and leaves |
| CN103931929B (en) * | 2014-03-25 | 2016-03-30 | 江苏长顺农牧有限公司 | A kind of feed preventing the global-worm illness of chicken |
| CN104489249A (en) * | 2014-05-09 | 2015-04-08 | 重庆达军食品有限责任公司 | Low-cholesterol ecological egg production method |
| CN104366143A (en) * | 2014-12-10 | 2015-02-25 | 成都市农林科学院 | Layer mulberry feed and production method thereof |
| CN105104855A (en) * | 2015-08-28 | 2015-12-02 | 马鞍山市五谷禽业专业合作社 | Anti-inflammatory bactericidal Chinese fan palm leaf fermented material for chicken and preparation method of Chinese fan palm leaf fermented material |
| CN105104854A (en) * | 2015-08-28 | 2015-12-02 | 马鞍山市五谷禽业专业合作社 | Chinese fan-palm leaf fermented feed for accelerating growth of chicken at initial and middle stages and preparation method thereof |
| CN105104729A (en) * | 2015-08-28 | 2015-12-02 | 马鞍山市五谷禽业专业合作社 | Chinese fan palm leaf fermented material capable of improving Chicken texture and preparation method of Chinese fan palm leaf fermented material |
| CN105124235A (en) * | 2015-08-28 | 2015-12-09 | 马鞍山市五谷禽业专业合作社 | Chicken egg production increasing livistona chinensis leaf fermentation feed for chicken and preparation method thereof |
| CN105285472A (en) * | 2015-11-20 | 2016-02-03 | 刘惠强 | Nutritious layer feed and preparation method thereof |
| CN105724820A (en) * | 2016-03-03 | 2016-07-06 | 刘自忠 | Chlorella broiler feed and feeding method thereof |
| CN106719321A (en) * | 2016-11-18 | 2017-05-31 | 扶绥县科学技术情报研究所 | The method that laying hen is raised using microorganism formulation |
| CN106721477A (en) * | 2016-12-23 | 2017-05-31 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of egg feedstuff special suitable for fermentation bed |
| CN107136312A (en) * | 2017-05-26 | 2017-09-08 | 山东金码头生物科技有限公司 | A kind of egg feedstuff |
| CN109362987B (en) * | 2018-11-07 | 2022-04-22 | 湖南桑叶加农业科技有限公司 | Poultry antibiotic-free mulberry leaf fermentation concentrated material and preparation method thereof |
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Inventor after: Wang Sulong Inventor before: Lai Guangye Inventor before: Huang Nangai Inventor before: Mo Mingxin Inventor before: Nong Xiangsheng |
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Effective date of registration: 20160418 Address after: 044000 snow road, Yuncheng city Shanxi Airport Development Zone Bureau of South Building Patentee after: Shanxi Jinlong group Yuncheng Feed Co. Ltd. Address before: 530300 Hengxian Productivity Promotion Center, Hengxian Town, Nanning, the Guangxi Zhuang Autonomous Region Patentee before: Lai Guangye |
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