The laying hen preparation method of microbial fermentation antibiotic-free feed
Technical field
The present invention relates to a kind of feed, particularly a kind of laying hen belongs to the fermentative feedstuff of microbe field with the preparation method of microbial fermentation antibiotic-free feed.
Background technology
More and more be subject to people's attention in the nowadays raising of quality of life, high-quality, environmental protection and green and natural grocery trade more are subject to people's favor.Egg is a main farm produce of China, also is the modal non-staple foodstuff kind of China resident, and along with improving constantly of living standard, people are when improving the egg consumption figure, and are also more and more higher to the quality requirements of egg.Now China's cultivation still relies on antibiotic present situation and can't satisfy people to the demand of healthy food, and along with antibiotic abuse, cause that antibody-resistant bacterium constantly increases, the antibiotic serious threat such as residual is to the problem of human health, caused everybody concern.
By in animal feed, adding useful living microorganism, have effect additional, that regulate, keep microbial balance in the enteron aisle, utilize the principle with bacterium bacterium processed, reach minimizing or need not antibiotic purpose.
Chinese patent (application number: 200810123515.5, denomination of invention: lactobacillus feedstuff for eggs) disclose a kind of lactobacillus feedstuff for eggs, include the chicken feed, add the lactic acid bacteria of its weight 0.1%~1% and shrubby sophora extract, hoary hair's extract, Herb Gynostemmae Pentaphylli extract in the chicken feed, it can be regulated the stomach normal flora, keep microecological balance, thereby improves function of intestinal canal; Improve digestibility and the biological value of food; Reduce serum cholesterol, the control endotoxin; Suppress corrupt bacteria growing in the enteron aisle; Improve immunity of organisms.Have nontoxic, without the resistance to the action of a drug, noresidue, the characteristics such as have no side effect.But because it adds single kind of bacterium, action effect is unstable.
Summary of the invention
The present invention adopts the biotechnology principle, utilize beneficial microbe can decompose, utilize carbon source, nitrogenous source in the daily ration to be converted into the characteristic of microbial bacteria body protein, and the feed after the fermentation is fragrant, the advantage of good palatability, can stimulate chicken appetite, improve efficiency of feed utilization, and improve micro-ecological environment in the chicken, suppress the growth of pathogenic bacteria in the body, improve physique and resistance against diseases, reach and completely abolish the purpose that the antibiotics medicine adds, the preparation method of a kind of laying hen with microbial fermentation antibiotic-free feed is provided.The method may further comprise the steps:
1. recovery: the microbial fermentation microbial inoculum is pressed laying hens essential ration percentage by weight 0.3~0.8% drop in the recovery container, add 25~30 ℃ warm water, amount of water is 2~4 times of described microbial fermentation microbial inoculum weight, and 8~12h is left standstill in sealing, forms recovery bacterium liquid;
Described recovery container is the closed container of adding a cover.
2. fermentation: the good bacterium liquid of above-mentioned steps 1 recovery is diluted to the bacterium liquid dilution of laying hens essential ration gross weight 30%, drops in the Zymolysis Equipment with laying hens essential ration, fully stir sealing fermentation 3~24h; Fermentation time is decided with room temperature, and the time in summer is slightly short, proper extension time in winter, best results when little sour fragrance appears in fermentation materials; For fully fermenting, need during the fermentation timing agitation, be spaced apart and per hour stir 10 minutes;
3. filter: after little sour fragrance appearred in fermentation materials, by screen pack separation and fermentation liquid and fermentation residue, fermentation residue is feeding directly.
Described microbial fermentation microbial inoculum is the feed fermenting composite bacteria agent capable that Guangzhou Nongguan Biotech Co., Ltd. develops, and its proterties is pulvis, total viable count 〉=1.0 * 10
9Cfu/g contains the useful fungal components such as lactic acid bacteria, saccharomycete, fatty bacterium, bacillus, peptide bacillus.
Guangzhou Nongguan Biotech Co., Ltd. is global transnational enterprise, is to introduce the advanced biotechnology in Taiwan, is devoted to the biologic product research and development, produces and sells the new high-tech enterprise that is integrated.Company's main management brand has " farming hat ", " farming is transported ", " garlands " three kinds, principal products of business have microorganism formulation raw material, microorganism purification of water quality, bio-feritlizer function bacterium, feed to add biologic product, organic fertilizer fermentation agent etc., and it is qualified that product quality detects through national authority mechanism.The specifying information of company can be understood by network address http://baike.baidu.com/view/5049834.htm.
The composite bacteria agent capable of the said firm's research and development is the demands according to China's feedstuff industry and livestock breeding industry, the probiotics of latest generation on the market of employing international advanced technology and device fabrication, it has the advantages such as breeding is quick, vitality is strong, safety non-toxic, fast decoupled organic matter, and can regulate animal intestinal thalline balance, improve intestine microenvironment, effectively keep the animal body health status, improving its immunity, resistance against diseases, anti-stress ability, is a kind of efficient animal feed fermenting agent.
Described laying hens essential ration does not add human body and harmful medicine and the chemical addition agent of animal, the parts by weight of its key component are: 580~700 parts of corn flour, 120~200 parts of bean cake powders, 120~200 parts in rapeseed dregs powder, 30~50 parts of mulberry leaf powders, 2~5 parts of salt, 3~6 parts of edible oils, 0.2~1 part of egg amino, 5~15 parts of calcium monohydrogen phosphates, 2~8 parts of stone flours, raw material all can buy in market.Described stone flour is calcium carbonate stone powder.
Mulberry leaf nutrient is abundant, be described as " natural nutrition storehouse ", its crude protein content reaches about 20%, also be rich in trace element and vitamin E, C, B1, B2, A and the letones such as potassium, calcium, copper, zinc, boron, manganese, in laying hen basis solar eclipse, add mulberry leaf powder, can improve laying hen egg yield, egg size and yolk color, significantly reduce the eggshell crack rate, increase total amino acid content and vitamin content.Guangxi is positioned at Southwestern China section, has the development silkworm and mulberry and produces advantageous condition, adds the support of national policy, now has been that the largest silkworm and mulberry sparetime university of China economizes, for the invention process provides abundant raw material resources.
Compared with prior art, the invention has the beneficial effects as follows: manufacture craft of the present invention is simple, and is low for equipment requirements, very easily promotes; Feed is without hormone, medicament residue, safety and environmental protection, and good palatability, nutrition absorbs easily, can reduce the feed consumption, reduces aquaculture cost; Prolong laying period, improve egg production, reduce the cholesterol level of egg ovum; Improve breeding environment, reduce the sick rate of sending out.
The specific embodiment
Specify below in conjunction with specific embodiment.
One, the laying hen preparation method of microbial fermentation antibiotic-free feed
Embodiment 1
1. recovery: take by weighing the feed fermenting composite bacteria agent capable 5kg that Guangzhou Nongguan Biotech Co., Ltd. develops, drop in the recovery container, add 25~30 ℃ of warm water, amount of water 15Kg fully stirs rear enclosed and leaves standstill 10h, forms recovery bacterium liquid;
2. fermentation: the bacterium liquid dilution that the good bacterium liquid of above-mentioned steps 1 recovery is diluted to 300kg, drop in the Zymolysis Equipment with the 1000Kg laying hens essential ration, fully stir the rear enclosed fermentation, in the sweat every 1h timing agitation 10min, so that the material fermentation fully, until little sour fragrance appears in fermentation materials, stop fermentation; Fermentation duration apparent temperature changes and adjusts, and summer is slightly short, and winter is slightly long, and fermentation time 3~24h does not wait, take feed fermentation to little sour fragrance as good.
3. filter: after little sour fragrance appearred in fermentation materials, by screen pack separation and fermentation liquid and fermentation residue, fermentation residue is feeding directly.
Wherein, laying hens essential ration does not add human body and harmful medicine and the chemical addition agent of animal, the parts by weight of its key component are: 618 parts of corn flour, 160 parts of bean cake powders, 160 parts in rapeseed dregs powder, 40 parts of mulberry leaf powders, 3 parts of salt, 5 parts of edible oils, amino 0.5 part of egg, 10 parts of calcium monohydrogen phosphates, 3.5 parts of stone flours, raw material is all bought from market.Described stone flour is calcium carbonate stone powder.
Two, application test
The feed that the below uses above-described embodiment 1 to make carries out the layer breeding contrast test with the antibiotic feed of interpolation.The selective body heavy phase is near, 100 of the laying hens of 300 ages in days, be divided at random experimental group and control group, the experimental group feed that embodiment 1 makes of feeding, the control group former basal diet (containing antibiotic) of only feeding carries out 30 days contrast tests such as laying rate, feedstuff-egg ratio and rate of broken eggs.Experiment periods adopts raises in cages, and selects every day in the morning 6 and orders feeding afternoon 3, guarantees the chicken free choice feeding.Microbial inoculum recovery and the fermenting raw materials arrangement of time of its experimental group are as follows:
1, evening, the 1:00 microbial inoculum of ordering was recovered, the fermentation of 11:00 point spice, and afternoon, the 15:00 point was fed.
2, the morning, the 11:00 microbial inoculum of ordering was recovered, the fermentation of 21:00 point spice, and the next morning, the 6:00 point was fed.
In the process of the test, every day, 17:00 carried out the data statistics of egg number, inlet amount, broken egg number.Through by a definite date 30 days test, the result adds up such as following table:
Index |
Control group |
Experimental group |
Chicken number (30d) |
1500 |
1500 |
Egg number (piece) |
1255 |
1371 |
Average egg production (%) |
83.67 |
92.73 |
Average egg kind (g/ piece) |
64.12 |
66.75 |
Total egg size (Kg) |
80.47 |
91.51 |
Total feed intake (Kg) |
173.89 |
179.07 |
Feedstuff-egg ratio |
2.16 |
1.96 |
Broken egg number (piece) |
9 |
6 |
Rate of broken eggs (%) |
0.72 |
0.44 |
Result of the test shows: the laying rate contrast group of experimental group improves 9.77%, and average egg weight improves 3.94%, and feedstuff-egg ratio reduces by 9.26, and rate of broken eggs reduces by 38.89%, remarkable in economical benefits; In the test feeding process, the environment of experimental group is better than control group, and laying period obtains prolonging, and the cholesterol of egg is compared with normal diet and to be had clear superiority.
Above-mentioned case study on implementation only illustrates specific embodiments of the present invention, limits absolutely not its actual range, and protection scope of the present invention is as the criterion with the claim protection domain.