CN110150495B

CN110150495B - Fully fermented livestock and poultry complete feed and preparation method and application thereof

Info

Publication number: CN110150495B
Application number: CN201910567565.0A
Authority: CN
Inventors: 杜明明; 全文韬; 崔佳; 全秀振
Original assignee: Huangshi Jiaxing Biotechnology Co ltd
Current assignee: Huangshi Jiaxing Biotechnology Co ltd
Priority date: 2019-06-27
Filing date: 2019-06-27
Publication date: 2022-09-13
Anticipated expiration: 2039-06-27
Also published as: CN110150495A

Abstract

The invention discloses a fully fermented livestock and poultry complete feed and a preparation method and application thereof, wherein the fully fermented livestock and poultry complete feed is prepared by scientifically selecting and mixing various raw materials and additives in the complete feed, during fermentation, all the raw materials in prepared complete powder are involved in fermentation, a proper fermentation microbial inoculum is selected, and the fermentation depth is controlled, so that the fully fermented livestock and poultry complete feed is obtainedComplete feed for livestock and poultry; the product indexes of the fully fermented livestock and poultry complete feed are as follows: water content 28-35%, viable count: 3-8X 10 ⁸ cfu/g, pH: 5.5-6.5, wherein the content of each microelement and macroelement reaches the minimum value +/-3% recommended by the American NRC nutritional standard, the content of crude protein is calculated as the minimum value +/-3% recommended by the American NRC nutritional standard on a dry basis, and the quality guarantee period is 150-180 days; the invention has scientific formula, simple preparation method and obvious effect, achieves the large-scale production and preparation of the fully fermented livestock and poultry complete feed and the actual production and application on livestock and poultry breeding, and is suitable for wide popularization and application.

Description

Fully fermented livestock and poultry complete feed and preparation method and application thereof

Technical Field

The invention relates to the technical field of animal feed preparation, in particular to a fully fermented livestock and poultry complete feed and a preparation method and application thereof.

Background

With the increasing environmental protection requirements of the country on animal breeding production and the increasing strict requirements on food safety such as meat, eggs and milk, the country forbids the use of antibiotics in the feed in 2020, which brings challenges to the stable production of animals. In this case, complete fermentation of complete feeds is one of the main methods to solve this problem. The full-fermentation complete feed refers to the complete fermentation complete feed in which all raw materials participate in the fermentation process, the successfully fermented complete fermentation complete feed contains various metabolic end products, such as small peptides, hydrolytic enzymes, antibacterial peptides, vitamin B and other series substances, and the viable count can reach (2-8) x 10 ⁸ cfu/g (the number of viable bacteria is also one of the standards for judging whether the complete fermentation complete feed is successful, and is related to whether the feed can realize the key factor of not adding antibiotics or not), wherein the hydrolase can improve the digestibility of the feed, and the antibacterial peptide substances can play a role similar to antibiotics, thereby achieving the purpose of antibiotic-free culture.

At present, complete fermentation is carried out on complete feed, and the technology is still immature, so that the complete feed cannot be applied to livestock and poultry feed. The main technical difficulties of carrying out comprehensive fermentation on the livestock and poultry complete feed include the following aspects:

1. too high salt content: at least 7-8 trace mineral raw materials and 2-3 constant mineral raw materials are usually required to be added into the complete feed, and most of the materials are currently added in the form of inorganic salts, so that the complete feed can cause death of beneficial flora due to overhigh salt content in the complete fermentation process, and finally cause incomplete fermentation or even fermentation failure (through detecting a finished product, almost no viable bacteria exist in the finished product, namely the fermentation failure).

2. The protein and energy raw materials have sufficient nutrient components: corn and soybean meal carbon source and nitrogen source raw materials in the complete feed are sufficient, and fermentation strains are not screened in the fermentation process, so that the complete feed is fermented excessively in the fermentation process, and the loss of nutrient substances is excessive (the point is opposite to the production of organic fertilizer, the more thorough fermentation is required in the production process of the organic fertilizer, the better fermentation is required, the proper fermentation is required for animal feed, and the reduction of the nutrient value of the feed due to the excessive consumption of the nutrient substances is avoided).

3. Too high acidity: in a common fermentation mode, the obtained full-fermentation complete feed has the advantages of over-low pH value (3.7-4.8), over-high acidity, poor palatability, non-positive animal feeding, damage to intestinal mucosa in an animal body, easy diarrhea and illness of the animal, and even diarrhea and death of serious animals.

4. Calcium phosphorus effects: the addition of the major mineral raw materials calcium and phosphorus added into the complete feed can influence the activity of the probiotics, and especially the addition of calcium has great influence on the reproduction and development of the probiotics. Calcium in the complete feed: the proportion of phosphorus is (1-2) to (0.7-1), and the proportion is designed for ensuring the animal body to fully absorb calcium and phosphorus, but according to the proportion, the addition amount of calcium and phosphorus in the traditional full-value feed is large (generally reaching 2.8% -4.0% of the total mass of the full-value feed, wherein 1.2% -1.8% of calcium hydrogen phosphate and 1.6% -2.2% of calcium carbonate or higher), the propagation of probiotics during full-value feed full fermentation can be seriously influenced, and simultaneously, the problem of over-standard phosphorus emission easily exists in animal excrement, so that the environmental pollution is caused.

5. Strain screening: the strain used in the fermentation process of the conventional single feed is generally aerobic, anaerobic and facultative compatible or aerobic and facultative compatible, and aims to improve the fermentation depth and automatically control over-fermentation by acidity so as to remove anti-nutritional factors, so that the acidity of the fermented feed is easily overhigh. In the process of fermenting the complete feed by full fermentation, both acidity and fermentation depth need to be controlled, because the feed is too high in acidity and cannot be directly fed, and the fermentation is excessive, so that the nutrient components are lost too much, and unnecessary waste is caused, therefore, the conventional method for fermenting single feed components cannot be applied to complete fermentation of the complete feed at present.

6. The shelf life is short: the common fermented feed is produced by a single feed raw material and is added into a complete feed in an amount of 5-10% for use, the fermented feed contains 25-40% of different moisture, the complete feed added with partial fermented feed is easy to cause moisture content increase, so the fermented feed is not beneficial to storage, the feed is easy to mildew and deteriorate in a short period (2-3 days in summer and 5-7 days in winter), the ingredients are troublesome, the use is inconvenient, and the fermented feed is also one of the reasons for resisting the use of many production enterprises.

In summary, the fully fermented complete feed for livestock and poultry, which has long quality guarantee period and low production cost and is simple and easy to produce, does not exist in the industry at present, and the research of the preparation method of the fully fermented complete feed for livestock and poultry becomes an urgent problem to be solved in the industry along with the improvement of the requirements of various aspects of animal breeding.

Disclosure of Invention

The invention aims to provide a fully fermented livestock and poultry complete feed and a preparation method and application thereof, aiming at solving the problems that the environmental protection requirement on the production process is higher and higher, the safety requirements on foods such as meat, eggs and milk are stricter and the use of antibiotics in the feed is forbidden comprehensively in the existing animal breeding industry, and a series of new problems bring challenges to the stable production of animals; the fully fermented livestock and poultry complete feed can solve a series of problems.

The invention relates to a fully fermented livestock and poultry complete feed, which consists of the following raw materials in percentage by weight:

(1) 60-75% of energy raw materials, wherein the energy raw materials are any one or two or more of corn, wheat, barley, sorghum, rice, oat, rice and rice bran, and any one or two or more of palm kernel meal, gordon euryale seed residue, tartary buckwheat residue, bran and potato powder;

(2) 20-28% of protein raw materials, wherein the protein raw materials are any one or two or more of soybean cake, meal, cotton seed cake, meal, rapeseed cake, meal, peanut kernel cake, meal, sunflower seed cake, meal, linseed cake, meal, sesame cake, meal, coconut meal, rice bran meal, corn protein powder, soybean protein powder, rice protein powder, DDGS (distillers dried grains with solubles), dry alfalfa meal, fish meal, meat bone meal, blood meal, feather meal, silkworm chrysalis meal and silkworm chrysalis meal;

(3) 1-4% of trace mineral raw material premix: the mineral in the trace mineral premix is a trace mineral premix with different proportions, which is designed according to different species and different physiological needs of animals, and is prepared from organic copper, organic iron, organic manganese, organic zinc, organic iodine, organic selenium and organic cobalt;

(4) 0.5-2% of a constant mineral raw material: the macromineral raw materials are calcium and phosphate rock substance salt, and the weight ratio of calcium: the mass ratio of the added phosphorus is (1-2) to (0.7-1); the adding proportion is designed for ensuring the animal body to fully absorb calcium and phosphorus; the proper amount of phosphorus and calcium is added into the complete feed for the fully fermented livestock and poultry according to the addition proportion, the growth and reproductive performance of animals are not influenced, and the complete fermentation of the complete feed is facilitated;

(5) 0.5-1% of composite vitamin premix: the compound vitamin premix is designed according to different species and different physiological requirements of animals, and has different types and proportions;

(6) salt: 0.15 to 0.25 percent;

(7) 0.05-0.1% of enzyme preparation: the enzyme preparation is any one or two or more of phytase, amylase, protease, lipase, cellulase, mannanase and xylanase;

(8) 0.05-0.1% of strain: the strain adopts aerobic bacteria, and the aerobic bacteria do not comprise facultative bacteria, aerobic bacteria with high acid yield, bacillus cereus and bacillus thuringiensis which influence the propagation of other bacteria;

the sum of the raw materials is 100 percent;

when in preparation, the raw materials to be crushed in the raw materials are respectively crushed to have the particle size of 0.6-3.0mm, so that the material structure is fluffy; sequentially feeding energy raw materials, protein raw materials, major mineral raw materials, salt, trace mineral premix, vitamin complex premix and enzyme preparation, uniformly stirring, inoculating the activated strain, performing aerobic fermentation for 36-96h under the environmental condition of 30-35 ℃, then filling into a breathing membrane bag, performing heat seal, and stopping fermentation automatically after oxygen in the bag is exhausted; the product indexes of the fully fermented livestock and poultry complete feed are as follows: water content 28-35%, viable count: 3-8X 10 ⁸ cfu/g, pH: 5.5-6.5, wherein each trace element and each macroelement reach the minimum value +/-3% recommended by the American NRC (2012 edition) nutrition standard, crude protein accounts for +/-3% of the minimum value recommended by the American NRC nutrition standard on a dry basis, and the quality guarantee period is 150-180 days.

The strain of the invention is: removing any one or two or more of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus pumilus and Bacillus circulans of high acid-producing strains.

In the trace mineral raw material premix in the raw materials, the organic copper is any one or two of methionine copper complex, lysine copper complex, glycine copper complex and yeast copper; the organic iron is one or two of methionine iron complex, glycine iron complex and yeast iron; the organic manganese is selected from one or two of methionine manganese complex and yeast manganese; the organic zinc is any one or two of zinc methionine complex, zinc lysine complex, zinc glycinate complex and zinc threonine complex; the organic iodine is calcium iodate; the organic selenium is selenium yeast; the organic cobalt is cobalt acetate.

The trace mineral raw materials in the invention are contained in the prepared full-fermentation complete feed as follows: 6-8ppm of organic copper, 80-120ppm of organic iron, 5-10ppm of organic manganese, 80-120ppm of organic zinc, 0.15-0.25ppm of organic iodine, 0.2-0.3ppm of organic selenium and 0.12-0.15ppm of organic cobalt.

The content of the macromineral raw materials in the prepared full-fermentation complete feed is as follows: 2.3-2.5ppm of calcium and 1.15-1.45ppm of total phosphorus.

The energy raw materials in the invention are slightly different from the traditional complete feed: the method is characterized in that the traditional energy raw materials such as corn, wheat and sorghum are selected, and raw materials such as palm kernel meal, tartary buckwheat slag and gordon euryale seed slag with high amylopectin rate are selected, so that the raw material selection range is enlarged, the cost of main raw materials (the tartary buckwheat slag, the gordon euryale seed slag and the like are byproducts for producing other products and are low in raw material price) is reduced, the cost of the feed can be reduced, the energy raw materials can meet the nutritional requirements of the finished feed after comprehensive fermentation, the fermentation depth can be controlled (the starch rate of the palm kernel meal, the tartary buckwheat slag powder and the like is more than 70%, but most of the energy raw materials are branched chain or crystalline starch, the fermentation degradation speed is low), and the problem that the nutritional ingredients are wasted due to the fact that the fermentation depth is not easy to control of the traditional energy raw materials is solved.

The protein raw material of the invention: a large number of plant protein raw materials with high anti-nutritional factors are selected, such as: the coconut meal, the sunflower meal, the peanut kernel meal, the dry alfalfa meal and the like reduce anti-nutritional factors through fermentation, improve the digestibility, reduce the feed cost and develop the raw material resources of protein feed.

The compound vitamin premix is selected according to the animal adapted to the prepared complete feed during the specific production, and a universal compound vitamin premix can be adopted. The main component contents of the three vitamin complex premixes are provided exemplarily in the invention.

1. The vitamin premix for livestock and poultry has the following vitamin contents in each kilogram: vitamin a 2900 ten thousand IU; vitamin D31000 ten thousand IU; vitamin E1.8 ten thousand IU; vitamin K34 g; vitamin B14 g; vitamin B218 g; vitamin B1240 mg; 1.8g of folic acid; 280mg of biotin; 40g of nicotinoyl phenol; 30g of D-pantothenic acid; vitamin B64 g; the water content is not higher than 10%.

2. The vitamin premix for the laying hens comprises the following vitamins in each kilogram: vitamin a 4400 ten thousand IU; vitamin D31000 ten thousand IU; vitamin E7 ten thousand IU; vitamin K310 g; vitamin B14 g; vitamin B220 g; vitamin B1240 mg; 3g of folic acid; 280mg of biotin; 110g of nicotinoyl phenol; 30g of D-pantothenic acid; vitamin B58 g; the water content is not higher than 10%.

3. The vitamin premix for the boars and the piglets comprises the following vitamins in each kilogram: vitamin a 4200 ten thousand IU; vitamin D3700 ten thousand IU; vitamin E5 ten thousand IU; vitamin K35 g; vitamin B15 g; vitamin B218 g; vitamin B61 g; vitamin B1260 mg; 1g of folic acid; 280mg of biotin; 60g of nicotinoyl phenol; 40g of D-pantothenic acid; the water content is not higher than 10%.

The product indexes of the fully fermented livestock and poultry complete feed are as follows: water content 28-35%, viable count: 3-8X 10 ⁸ cfu/g, pH: 5.5-6.5, wherein each microelement and macroelement reaches the minimum value +/-3% recommended by the American NRC nutrient standard, the crude protein is calculated as the minimum value +/-3% recommended by the American NRC nutrient standard on a dry basis, and the shelf life is 150-180 days. In the above standards, the reason why the standard values of the trace elements, the macroelements and the crude protein are limited to be less than the minimum value +/-3% recommended by the American NRC nutritional standard is that the complete feed disclosed by the invention is more beneficial to the absorption of animals after being fully fermented, so that the culture effect can be achieved even if the standard value is less than 3% of the recommended value, and the necessity of fully fermenting the complete feed is proved from the other aspect.

The preparation method of the fully fermented livestock and poultry complete feed comprises the following steps:

(1) according to a production plan, calculating the mass of each required raw material, weighing, and respectively crushing the raw materials to be crushed into particles with the size of 0.6-3.0mm to ensure that the material structure is fluffy; uniformly mixing the raw materials to be prepared into premix with a carrier to prepare the premix; sequentially feeding the energy raw material, the protein raw material, the macromineral raw material, the salt, the trace mineral premix, the multivitamin premix and the enzyme preparation, and uniformly stirring to obtain complete powder;

(2) activating the required strain with 2.5% brown sugar water for 8-12 hr to obtain viable bacteria with viable count of (100- ⁸ Activated bacterial liquid of cfu/ml,transferring the stirred full-valence powder into a inoculation machine, spraying activated bacterial liquid into the full-valence powder according to the proportion of 350-400kg of activated bacterial liquid in each ton of the material, and uniformly stirring;

(3) uniformly laying the inoculated materials on a fermentation bed for aerobic fermentation, wherein the laying thickness is 5-10cm, controlling the fermentation temperature to be 30-35 ℃ after the laying is finished, carrying out aerobic fermentation for 3-4 days until the moisture of the materials reaches 28-35% and the temperature of the materials is close to the ambient temperature, and finishing the aerobic fermentation;

(4) putting the fermented material into a breathing membrane bag, sealing in a heat seal manner, continuing aerobic fermentation of the material in the breathing membrane bag until oxygen in the breathing membrane bag is exhausted, and stopping fermentation automatically to obtain the fermented feed; the product indexes of the prepared fully fermented livestock and poultry complete feed are as follows: water content 28-35%, viable count: 3-8X 10 ⁸ cfu/g, pH: 5.5-6.5, wherein each trace element and macroelement reach the minimum value +/-3% recommended by the American NRC nutrition standard, the crude protein is calculated as the minimum value +/-3% recommended by the American NRC (2012 edition) nutrition standard on a dry basis, and the quality guarantee period is 150-plus 180 days.

Preferably, the activated bacterium liquid contains bacillus amyloliquefaciens (50-100) multiplied by 10 ⁸ cfu/ml, containing Bacillus licheniformis (30-150). times.10 ⁸ cfu/ml, containing Bacillus pumilus (20-50). times.10 ⁸ cfu/ml。

Preferably, the activated bacterium liquid contains bacillus subtilis (60-150). times.10 ⁸ cfu/ml, containing Bacillus licheniformis (20-80). times.10 ⁸ cfu/ml, containing Bacillus amyloliquefaciens (20-70). times.10 ⁸ cfu/ml。

Preferably, the activated bacterium liquid contains bacillus amyloliquefaciens (80-180) multiplied by 10 ⁸ cfu/ml, containing Bacillus brevis (10-60). times.10 ⁸ cfu/ml, containing Bacillus circulans (10-60). times.10 ⁸ cfu/ml。

In the preparation method, the fermentation bed adopted in the step (3) is a tunnel-type fermentation bed, the distance between the fermentation bed and the bottom of the tunnel is 40-60cm, the width of the tunnel is 150cm, the length of the tunnel is 600cm, the width of the fermentation bed is 150cm, the length of the fermentation bed is 550cm, the temperature-controlled oxygen supply fan is arranged at one end of the tunnel below the fermentation bed, the insertion depth of a temperature-controlled probe of the temperature-controlled oxygen supply fan into the material is 2-5cm, and the distance between the temperature-controlled probe and the fan is 400 cm. The invention adopts the tunnel type fermentation bed for aerobic fermentation, mainly because the tunnel type fermentation bed can well control the fermentation temperature and the moisture of the material, and the problems of excessive fermentation of the material and loss of nutritional factors caused by bitter taste of the material due to low-temperature fermentation or high-temperature fermentation are avoided. Other fermentation beds are also feasible, in case fermentation conditions can be reached.

The invention relates to application of a fully fermented livestock and poultry complete feed in livestock and poultry feed.

The invention relates to an application of a fully fermented livestock and poultry complete feed in livestock and poultry feed, wherein the livestock feed refers to pig feed, cattle feed, sheep feed or rabbit feed; the poultry feed refers to laying hen feed or laying duck feed or broiler feed or meat duck feed.

The invention relates to a full-fermentation complete feed which is obtained after years of continuous tests and innovation by an inventor, so that the faced problems are all brand-new problems, even the existing problems, and are summarized after the inventor is combined with actual production in the continuous test process, and in order to discover and solve six problems mentioned in the background technology, the technical scheme of the invention adopts the following technical means to respectively overcome the problems listed in the background technology:

1. the problem of in the traditional complete feed salinity too high result in fermentation failure is solved: the trace mineral raw material in the raw material is changed into the organic mineral chelate, so that the method has four advantages: a. the problem of fermentation failure caused by overhigh salt content caused by inorganic mineral salt is solved; b. the addition of mineral elements is greatly reduced, and the organic mineral chelate is an organic matter and is beneficial to the absorption of animal organisms, so that the animal nutrition requirement can be met only by adding a small amount of the organic mineral chelate; c. in the fermentation process, the metabolic end products can be chelated with mineral substances existing in the raw materials, so that more organic mineral substance chelates are generated, and the utilization rate of mineral elements in the feed is maximized; d. the excretion of mineral elements in the livestock and poultry manure is reduced, and the influence of the breeding industry on the natural environment is further reduced.

2. Adjusting the selection range of energy raw materials, and controlling the fermentation depth: except for reserving traditional energy raw materials such as corn, wheat and the like, new energy raw materials (high amylopectin rate) are introduced: such as palm kernel dregs, buckwheat dregs powder, gorgon fruit dregs powder, etc. By doing so, the feed cost can be reduced, the energy raw materials can meet the nutritional requirements of the finished feed after complete fermentation, the fermentation depth can be controlled (such as palm kernel meal, tartary buckwheat residue powder and the like, the starch rate is more than 70%, but most of the starch is branched chain or crystalline starch, the fermentation degradation speed is slow), and the problem of nutrient component waste caused by difficulty in controlling the fermentation depth of the traditional energy raw materials is avoided.

3. Screening strains to avoid over-low acidity after fermentation of complete feed: through numerous tests and production practices, the aerobic fermentation strain with low acid production is selected as the fermentation microbial inoculum, so that the problems of overhigh acidity, poor palatability and easy animal diarrhea caused by comprehensive fermentation of complete feed are solved, the problem of short shelf life of finished feed is also solved (only aerobic bacteria are adopted, facultative bacteria and anaerobic bacteria are eliminated, and the problem of feed deterioration caused by continuous fermentation after the complete feed is packaged after fermentation is avoided), and an aerobic fermentation bed which is self-developed by the applicant is adopted in the fermentation process to better control the fermentation depth.

4. The calcium-phosphorus ratio is as follows: the calcium and phosphorus addition amount in the traditional complete feed is higher, the general calcium and phosphorus addition ratio is (1-2): 0.7-1), the addition ratio is favorable for animals to absorb calcium and phosphorus better, but according to the ratio, the normal calcium and phosphorus addition amount in the traditional complete feed can cause the failure of complete fermentation of the complete feed, and the main reason is that beneficial strains can not be bred well. In addition, due to the fact that the digestion and absorption capacity of animals is limited, excrement excreted by the animals contains a large amount of phosphorus, and the ecological environment is damaged, the phytase is added and combined with fermentation, most of organic phosphorus can be resolved from raw materials, the addition amount of the phosphorus is reduced, and the addition proportion of calcium is reduced accordingly.

The fully fermented livestock and poultry complete feed has the following advantages:

1. controlling acidity, and can be directly fed. The feed palatability is not affected by over-high acidity, and even the animal is not diluted.

2. Improves the digestibility of the animals to the feed (various digestive enzymes are generated due to fermentation), thereby improving the feed-meat ratio/feed-egg ratio of the animals and improving the culture benefit.

3. The feed is convenient to use, can be fed after the package is opened, and has a greatly prolonged shelf life, so that the fully fermented complete feed can be practically applied in production.

4. The feed raw material resources are developed, energy raw materials with high amylopectin rate and protein raw materials with high anti-nutritional factors can be used, the raw material cost is reduced, and the diversity of feed raw material selection is enriched.

5. Reduces the discharge of ammonia nitrogen and trace mineral substances and phosphorus, is beneficial to environmental protection, and reduces the environmental protection pressure in enterprise production.

6. The nonreactive breeding is really realized, the food safety of animal products is ensured, and the flavor of the animal products can be improved.

Detailed Description

Example 1

In the embodiment, the invention is explained in detail by taking the example that 2.5 tons of lean-type fattening pig complete fermentation complete feed is produced by jiaxing biotechnology limited company in yellow stone city in 2018 for 5 months, wherein the initial complete feed formula for the fattening pig in the embodiment comprises the following raw materials in percentage by weight:

energy raw material 75%: comprises 46.6 percent of corn, 5 percent of palm kernel meal, 13 percent of paddy, 5.4 percent of gordon euryale seed residue and 5 percent of tartary buckwheat residue;

protein raw material 21.5%: comprises 14 percent of soybean meal, 4 percent of domestic fish meal and 3.5 percent of dry alfalfa meal;

premix of trace mineral raw materials: 0.5 percent, in this example, the trace mineral raw material premix is prepared by using copper methionine, zinc lysine, iron glycinate, manganese methionine, calcium iodate, selenium yeast and cobalt acetate as raw materials, and each kilogram of the used trace mineral raw material premix contains: 12-16g of copper, 80-120g of zinc, 160g of iron 100-.

Macromineral raw materials: 1.7%: wherein, the calcium hydrophosphate is 0.8 percent, and the calcium carbonate is 0.9 percent.

The compound vitamin premix comprises the following components: 0.5 percent, the embodiment adopts the vitamin premix of the breeding pigs and the piglets, and each kilogram of the premix contains the following vitamins: vitamin a 4200 ten thousand IU; vitamin D3700 ten thousand IU; vitamin E5 ten thousand IU; vitamin K35 g; vitamin B15 g; vitamin B218 g; vitamin B61 g; vitamin B1260 mg; 1g of folic acid; 280mg of biotin; 60g of nicotinoyl phenol; 40g of D-pantothenic acid; water content is not higher than 10%;

salt: 0.25 percent;

enzyme preparation: 0.5 percent, and the activity of the phytase is more than or equal to 5000U/g; amylase is more than or equal to 100U/g; the neutral protease is more than or equal to 1500U/g; the xylanase accounts for more than or equal to 1000U/g and comprises the following components in percentage by weight: phytase 0.2%, amylase and neutral protease and xylanase 0.3%;

bacterial agent: 0.05 percent, 0.02 percent of bacillus amyloliquefaciens, 0.02 percent of bacillus licheniformis and 0.01 percent of bacillus pumilus.

(1) calculating the mass of each required raw material according to a production plan, weighing, and respectively crushing the raw materials to be crushed into particles with the size of 0.6-1.0mm to ensure that the material structure is fluffy; uniformly mixing the raw materials to be prepared into premix with a carrier to prepare the premix; sequentially feeding the energy raw material, the protein raw material, the macromineral raw material, the salt, the trace mineral premix, the vitamin premix and the enzyme preparation, and uniformly stirring to obtain complete powder;

(2) activating the required strain with 2.5% brown sugar water for 10 hr to obtain viable bacteria with a viable bacteria count of (200-300) x 10 ⁸ Transferring the stirred full-value powder into an inoculating machine according to 380kg of activated bacterial liquid per ton of materialProportionally, spraying activated bacteria liquid into the complete powder, and uniformly stirring;

(3) uniformly laying the inoculated materials on a fermentation bed for aerobic fermentation, wherein the laying thickness is 5-8cm, controlling the fermentation temperature to be 30-35 ℃ after the laying is finished, carrying out aerobic fermentation for 3.5 days until the moisture of the materials reaches 31% and the temperature of the materials is close to the ambient temperature, and finishing the aerobic fermentation;

(4) putting the fermented material into a breathing membrane bag, sealing by heat seal, continuing aerobic fermentation of the material in the breathing membrane bag until oxygen in the breathing membrane bag is exhausted, and stopping fermentation automatically; the product indexes of the fully fermented livestock and poultry complete feed prepared by the embodiment are as follows: the water content is less than or equal to 32 percent, the viable count: 4-7X 10 ⁸ cfu/g, pH: 5.8-6.2, the crude protein is more than or equal to 15 percent, and the quality guarantee period is 150-; the content of trace mineral raw materials in the prepared full-fermentation complete feed is as follows: 6-8ppm of copper, 50-80ppm of iron, 5-10ppm of manganese, 40-60ppm of zinc, 0.15-0.25ppm of iodine, 0.2-0.3ppm of selenium and 0.12-0.15ppm of cobalt; the content of the macromineral raw materials in the prepared full-fermentation complete feed is as follows: 2.3-2.35ppm of calcium and 1.15-1.2ppm of total phosphorus; the trace element content reaches the minimum value specified in the us NRC (2012 edition).

In this example, the activated bacterial solution contains Bacillus amyloliquefaciens (50-100). times.10 ⁸ cfu/ml, containing Bacillus licheniformis (30-150). times.10 ⁸ cfu/ml, containing Bacillus pumilus (20-50). times.10 ⁸ cfu/ml。

In the preparation method, the fermentation bed adopted in the step (3) is a tunnel-type fermentation bed, the distance between the fermentation bed and the bottom of the tunnel is 60cm, the width of the tunnel is 150cm, the length of the tunnel is 600cm, the width of the fermentation bed is 150cm, the length of the fermentation bed is 550cm, a temperature-control oxygen supply fan is arranged at one end of the tunnel below the fermentation bed, the depth of a temperature-control probe of the temperature-control oxygen supply fan inserted into a material is 4cm, and the distance between the temperature-control probe and the fan is 350 cm.

In order to verify the breeding effect of the fully fermented complete-price pig feed prepared in the embodiment, 40 fattening pigs of 30-60kg are selected and subjected to a feeding test for 45 days, and the fattening pigs are reasonably divided into A, B groups according to the weight and the development condition, wherein each group comprises 20 fattening pigs, each group comprises 2 piggeries for feeding, and each piggery comprises 10 fattening pigs.

The group a is a control group, the group B is a test group, the group a is fed with a complete pig feed (only crushed, mixed and not fermented) having the same initial nutrient content as the present example, the group B is fed with the complete fermented complete pig feed prepared in the present example, and the specific test results are as shown in the following table one:

table one: control experiment results of feeding of full-fermentation complete pig feed prepared in example 1 and traditional complete pig feed

As can be seen from table one above: the fattening pig head daily feed intake of the group A control group is 1.747Kg, the head daily weight gain is 0.680g, the average weight gain is 30.1Kg, and the average feed-meat ratio is 2.57: 1, prevalence rate 10%; the fattening pigs in the group B are fed with 1.927Kg daily head feed intake, 0.18Kg is fed compared with the control group, the daily head feed gain is 0.768Kg, the daily head feed gain is 0.088Kg, the final average weight gain is 34.56Kg, the daily tail feed gain is 4.46Kg, and the average feed-meat ratio is 2.51: 1, the disease rate is 0 percent and 10 percent less than that of the control group, and the weight of the traditional Chinese medicine composition is 0.06 kg. Through the comparative analysis, it can be seen that when the fully fermented complete feed of the embodiment is used for carrying out a fattening pig feeding test, pigs prefer to eat the fully fermented complete feed prepared by the embodiment, meanwhile, the daily gain is fast, the feed conversion ratio is low, the morbidity is also low, the pigs live and are strong, the pigsty has no ammonia smell, the pig manure has no odor, and mosquitoes and flies are obviously reduced. The full-fermentation complete feed produced by the method can completely replace the traditional granulated or non-granulated complete feed, and the feeding effect is better than that of the traditional feed.

Example 2

In this example, the present invention is explained in detail by taking an example of producing a 0-4 week old white feather fast-growing large broiler feed by jiaxing biotechnology limited company in yellow stone city in 2018 for 5 months. The initial complete feed formula of the 0-4 week-old white feather fast large broiler in the embodiment comprises the following raw materials in parts by weight:

energy raw material 69.1%: comprises 58.1 percent of corn, 5 percent of palm kernel meal and 6 percent of gordon euryale seed residue;

protein raw material 27.1%: comprises 15% of soybean meal, 5% of domestic fish meal, 3% of imported meat and bone meal and 4.1% of rapeseed meal;

premix of trace mineral raw materials: 0.5 percent, in the embodiment, copper lysine, zinc methionine, iron yeast, manganese yeast, calcium iodate, selenium yeast and cobalt acetate are used as the raw materials of the trace mineral raw material premix. In the premix of trace mineral raw material used in this example, per kg of premix contained: 12-13g of copper, 80-120g of zinc, 130g of iron 110-.

Macromineral raw materials: 2%: calcium hydrogen phosphate 0.9 wt% and calcium carbonate 1.1 wt%

The compound vitamin premix comprises the following components: 0.5 percent, in the embodiment, the universal vitamin premix for livestock and poultry is adopted, and the content of each vitamin in each kilogram is as follows: vitamin a 2900 ten thousand IU; vitamin D31000 ten thousand IU; vitamin E1.8 ten thousand IU; vitamin K34 g; vitamin B14 g; vitamin B218 g; vitamin B1240 mg; 1.8g of folic acid; 280mg of biotin; 40g of nicotinoyl phenol; 30g of D-pantothenic acid; vitamin B64 g; the water content is not higher than 10%.

Salt: 0.25 percent;

enzyme preparation: 0.5 percent, and the phytase activity is more than or equal to 5000U/g; amylase is more than or equal to 100U/g; the neutral protease is more than or equal to 1500U/g; the xylanase accounts for 0.1 percent of phytase, 0.4 percent of amylase, neutral protease and 0.1 percent of xylanase;

bacterial agent: 0.05 percent of bacillus subtilis, 0.02 percent of bacillus licheniformis and 0.01 percent of bacillus amyloliquefaciens;

the preparation method of the fully fermented complete chicken feed comprises the following steps:

(1) calculating the mass of each required raw material according to a production plan, weighing, and respectively crushing the raw materials to be crushed into particles with the size of 0.6-1.0mm to ensure that the material structure is fluffy; uniformly mixing the raw materials to be prepared into premix with a carrier to prepare the premix; sequentially feeding the energy raw material, the protein raw material, the macromineral raw material, the salt, the trace mineral premix, the vitamin and the enzyme preparation premix in sequence, and uniformly stirring to obtain complete powder;

(2) activating the required strain with 2.5% brown sugar water for 12 hr to obtain viable bacteria with viable count of (10-30) x 10 ⁹ Transferring the stirred full-value powder into an inoculation machine, mixing the activated bacterial liquid with cfu/ml activated bacterial liquid according to the proportion of 400kg of activated bacterial liquid per ton of material, and uniformly stirring;

(3) uniformly laying the inoculated materials on a fermentation bed for aerobic fermentation, wherein the laying thickness is 5-10cm, controlling the fermentation temperature to be 30-35 ℃ after the laying is finished, carrying out aerobic fermentation for 4 days, ensuring that the moisture of the materials reaches 34% and the temperature of the materials is close to the ambient temperature, and finishing the aerobic fermentation;

(4) putting the fermented material into a breathing membrane bag, sealing in a heat seal manner, continuing aerobic fermentation of the material in the breathing membrane bag until oxygen in the material is exhausted, and stopping fermentation to obtain the fermented feed; the product indexes of the fully fermented complete feed prepared by the embodiment are as follows: water content less than 35%, viable count 6-7X 10 ⁸ cfu/g, pH value of 5.7-6.3, crude protein content of more than or equal to 16.5%, and quality guarantee period of 150-; the contents of trace mineral elements in this example are as follows: 6-6.5ppm of copper, 40-45ppm of zinc, 55-65ppm of iron, 9.5-10ppm of manganese, 0.15-0.2ppm of iodine, 0.25-0.3ppm of selenium and 0.12-0.15ppm of cobalt. Macromineral raw materials, namely 0.9 percent of calcium hydrophosphate and 1.1 percent of calcium carbonate; the vitamin complex premix reaches the American NRC recommended standard value.

Preferably, the activated bacterial liquid in the preparation method contains bacillus subtilis (60-150). times.10 ⁸ cfu/ml, containing Bacillus licheniformis (20-80). times.10 ⁸ cfu/ml, containing Bacillus amyloliquefaciens (20-70). times.10 ⁸ cfu/ml。

In the preparation method, the fermentation bed adopted in the step (3) is a tunnel-type fermentation bed, the distance between the fermentation bed and the bottom of the tunnel is 40-60cm, the width of the tunnel is 150cm, the length of the tunnel is 600cm, the width of the fermentation bed is 150cm, the length of the fermentation bed is 550cm, the temperature-controlled oxygen supply fan is arranged at one end of the tunnel under the fermentation bed, the insertion depth of a temperature-controlled probe of the temperature-controlled oxygen supply fan into the material is 2-5cm, and the distance between the temperature-controlled probe and the fan is 400 cm.

The fully fermented complete chicken feed prepared in this example and the fully fermented complete chicken feed prepared in example 3 were subjected to broiler breeding test, and the test conditions and test results are detailed in example 3.

Example 3

This example illustrates the present invention in detail by taking the example of producing 5-9 week old white feather fast-growing broiler feed by jiaxing biotechnology limited company in yellow stone city in 2018 for 6 months. The initial complete feed formula of the 0-4 week-old white feather fast large broiler in the embodiment comprises the following raw materials in parts by weight:

70.1% of energy raw material: comprises 54.1 percent of corn, 8 percent of palm kernel meal and 8 percent of gordon euryale seed residue;

protein raw material 26.1%: comprises 9.5 percent of soybean meal, 3.1 percent of domestic fish meal, 7 percent of rapeseed meal, 5 percent of cottonseed meal and 1.5 percent of imported meat and bone meal;

premix of trace mineral raw materials: 0.5 percent, in the embodiment, copper glycinate, zinc lysine, iron methionine, manganese methionine, calcium iodate, selenium yeast and cobalt acetate are used as the raw materials of the trace mineral premix. In the premix of trace mineral raw material used in this example, per kg of premix contained: 12-13g of copper, 80-90g of zinc, 110g of iron 100-plus, 18-20g of manganese, 0.3-0.4g of iodine, 0.5-0.6g of selenium and 0.24-0.3g of cobalt;

macromineral raw materials: 2%: 0.95 percent of calcium hydrophosphate and 1.05 percent of calcium carbonate;

the compound vitamin premix comprises the following components: 0.5 percent, in the embodiment, the vitamin premix compound which is commonly used by livestock and poultry is adopted, and the content of each vitamin in each kilogram is as follows: vitamin a 2900 ten thousand IU; vitamin D31000 ten thousand IU; vitamin E1.8 ten thousand IU; vitamin K34 g; vitamin B14 g; vitamin B218 g; vitamin B1240 mg; 1.8g of folic acid; 280mg of biotin; 40g of nicotinoyl phenol; 30g of D-pantothenic acid; vitamin B64 g; water content is not higher than 10%;

salt: 0.25 percent;

enzyme preparation: 0.5 percent, and the phytase activity is more than or equal to 5000U/g; amylase is more than or equal to 100U/g; the neutral protease is more than or equal to 1500U/g; the xylanase accounts for 0.2 percent of phytase, 0.3 percent of amylase, neutral protease and 0.3 percent of xylanase;

bacterial agent: 0.05 percent, 0.02 percent of bacillus subtilis, 0.02 percent of bacillus licheniformis and 0.01 percent of bacillus amyloliquefaciens.

(1) calculating the mass of each required raw material according to a production plan, weighing, and respectively crushing the raw materials to be crushed into particles with the particle size of 1.0-3.0mm to ensure that the material structure is fluffy; uniformly mixing the raw materials to be prepared into premix with a carrier to prepare the premix; sequentially feeding the energy raw material, the protein raw material, the macromineral raw material, the salt, the trace mineral premix, the vitamin and the enzyme preparation premix in sequence, and uniformly stirring to obtain complete powder;

(2) activating the required strain with 2.5% brown sugar water for 8 hr to obtain viable bacteria with viable count of (10-30) x 10 ⁹ Transferring the stirred full-value powder into an inoculation machine according to the activated bacterial liquid of cfu/ml, mixing the activated bacterial liquid into the full-value powder according to the proportion of 350kg of the activated bacterial liquid per ton of the material, and uniformly stirring;

(3) uniformly laying the inoculated materials on a fermentation bed for aerobic fermentation, wherein the laying thickness is 5-10cm, controlling the fermentation temperature to be 30-35 ℃ after the laying is finished, carrying out aerobic fermentation for 3 days, controlling the moisture of the materials to reach 33%, and finishing the aerobic fermentation when the temperature of the materials is close to the ambient temperature;

(4) putting the fermented material into a breathing membrane bag, sealing in a heat seal manner, continuing aerobic fermentation of the material in the breathing membrane bag until oxygen in the material is exhausted, and stopping fermentation to obtain the fermented feed; the product indexes of the fully fermented complete feed prepared by the embodiment are as follows: water content less than or equal to 34%, viable count of 7-8X 10 ⁸ cfu/g, pH value 5.5-6.0, crude protein content not less than 14%, quality guarantee period 150-; the trace mineral elements in this example contained the following: 6-6.5ppm of copper, 40-45ppm of zinc, 50-55ppm of iron, 9-10ppm of manganese, 0.15-0.2ppm of iodine, 0.25-0.3ppm of selenium and 0.12-0.15ppm of cobalt; 0.95 percent of macromineral raw material calcium hydrophosphate and 1.05 percent of calcium carbonate; the vitamin complex premix reaches the American NRC recommended standard value.

Preferably, the activated bacteria liquid in the preparation method contains bacillus subtilisBacillus (60-150). times.10 ⁸ cfu/ml, containing Bacillus licheniformis (20-80). times.10 ⁸ cfu/ml, containing Bacillus amyloliquefaciens (20-70). times.10 ⁸ cfu/ml。

In order to verify the breeding effect of the fully fermented complete chicken feeds prepared in examples 2 and 3, the fully fermented complete chicken feeds prepared in examples 2 and 3 and the unfermented complete chicken feed granules with the same formula as the fully fermented complete chicken feeds prepared in examples 2 and 3 were used in this example to perform a comparative test, and the details of the test are as follows: selecting 1000 white feather big fast broilers (male and female mixed seedlings) of 0 week age, reasonably dividing into A, B groups, each group comprising 500 broilers, wherein the group A is a control group, and feeding with unfermented complete feed of 0-4 week age and 5-9 week age according to the formula of the example 2 and the example 3; group B was a control group, and was fed with the 0-4 week-old and 5-9 week-old fully fermented complete feeds prepared in examples 2 and 3, and the feeding test was carried out for 63 days, and other controls were carried out according to normal control. The test results are shown in table two below:

table two: example 2 and example 3, the results of the control experiment (male and female mixed seedlings) on the feeding of the fully fermented full-value fast large white feather broiler feed prepared in example 2 and the conventional full-value fast large white feather broiler feed

As can be seen from table two above: the average daily feed intake of the group A is 55.2Kg, the average daily gain of the group is 24.3Kg, the end weight of the group is 1532.9Kg, and the average feed-meat ratio is 2.27: 1, prevalence 13.8%; the average daily feed intake of the test group B is 56.85Kg, 1.65Kg is more fed than the control group, the average daily weight of the group is 26.05Kg, 1.75Kg is more increased than the control group daily, the weight of the group is 1685.5Kg, 109.5Kg is more increased than the control group final weight, and the average feed-meat ratio is 2.18: 1, the disease rate is saved by 0.09kg compared with the control group, the disease rate is 0.5 percent, and the disease rate is reduced by 13.3 percent compared with the control group. Through the comparative analysis, the feeding test of the fast large white feather broilers by adopting the fully fermented complete feed of the embodiment has the advantages of fast weight gain, low feed conversion ratio, low morbidity, live chicken , health and health, no ammonia odor in chicken houses, no odor of chicken manure and obvious reduction of mosquitoes and flies. The method for producing the full-fermentation complete feed has the advantages that the traditional granulated or non-granulated complete broiler feed is replaced by the full-fermentation complete feed produced by the method, and the breeding effect is better.

Example 4

This example illustrates the present invention in detail by taking the example of 0-8 week old Qingyuan pheasant feed produced by Jiaxing biotechnology limited company of Huangshi city in 2018 for 3 months. The initial complete feed formula of the Qingyuan partridge chickens of 0-8 weeks old in the embodiment comprises the following raw materials in parts by weight:

70% of energy raw material: comprises 43 percent of corn, 5.2 percent of palm kernel meal, 6 percent of gordon euryale seed residue, 5.3 percent of tartary buckwheat residue and 10 percent of paddy;

26.7% of protein raw material: comprises 10 percent of soybean meal, 11 percent of peanut kernel meal, 3.5 percent of domestic fish meal and 2.2 percent of imported meat and bone meal;

premix of trace mineral raw materials: 0.5 percent, in the embodiment, copper methionine, zinc lysine, iron glycinate, manganese methionine, calcium iodate, selenium yeast and cobalt acetate are used as the raw materials of the trace mineral raw material premix. The premix of trace mineral raw material used in this example contained per kg: 6-6.5g of copper, 80-90g of zinc, 110g of iron 100-one, 17-20g of manganese, 0.3-0.4g of iodine, 0.5-0.6g of selenium and 0.24-0.3g of cobalt;

macromineral raw materials: 2%: 0.9 percent of calcium hydrophosphate and 1.1 percent of calcium carbonate.

The compound vitamin premix comprises the following components: 0.5 percent of vitamin premix compound which is commonly used by livestock and poultry, wherein each kilogram of vitamin premix compound contains the following vitamins: vitamin a 2900 ten thousand IU; vitamin D31000 ten thousand IU; vitamin E1.8 ten thousand IU; vitamin K34 g; vitamin B14 g; vitamin B218 g; vitamin B1240 mg; 1.8g of folic acid; 280mg of biotin; 40g of nicotinoyl phenol; 30g of D-pantothenic acid; vitamin B64 g; the water content is not more than 10 percent

Salt: 0.25 percent;

enzyme preparation: 0.5 percent, and the phytase activity is more than or equal to 5000U/g; amylase is more than or equal to 100U/g; the neutral protease is more than or equal to 1500U/g; xylanase more than or equal to 1000U/g, the proportion of the xylanase is that, phytase is 0.2 percent, amylase, neutral protease and xylanase are 0.3 percent

(1) calculating the mass of each required raw material according to a production plan, weighing, and respectively crushing the raw materials to be crushed into particles with the size of 0.6-1.5mm to ensure that the material structure is fluffy; uniformly mixing the raw materials to be prepared into premix with a carrier to prepare the premix; sequentially feeding the energy raw material, the protein raw material, the macromineral raw material, the salt, the trace mineral premix, the vitamin and the enzyme preparation premix in sequence, and uniformly stirring to obtain complete powder;

(2) activating the required strain with 2.5% brown sugar water for 12 hr to obtain viable bacteria with viable count of (10-30) x 10 ⁹ Transferring the stirred full-value powder into an inoculation machine according to the activated bacterial liquid of cfu/ml, mixing the activated bacterial liquid into the full-value powder according to the proportion of 390kg of activated bacterial liquid per ton of material, and uniformly stirring;

(3) uniformly laying the inoculated materials on a fermentation bed for aerobic fermentation, wherein the laying thickness is 6-8cm, controlling the fermentation temperature to be 30-35 ℃ after the laying is finished, carrying out aerobic fermentation for 4 days, controlling the moisture of the materials to be 32% and finishing the aerobic fermentation when the temperature of the materials is close to the ambient temperature;

(4) putting the fermented material into a breathing membrane bag, sealing by heat seal, continuing aerobic fermentation of the material in the breathing membrane bag until oxygen in the material is exhausted, and stopping fermentation to obtain the fermented material; the product indexes of the fully fermented complete feed prepared by the embodiment are as follows: water content less than or equal to 32%, viable count of 7-8X 10 ⁸ cfu/g, pH value of 5.8-6.4, crude protein content of more than or equal to 16%, and quality guarantee period of 150-; book blockThe contents of trace mineral elements in the examples are as follows: 6-6.5ppm of copper, 45-50ppm of zinc, 50-55ppm of iron, 8.5-10ppm of manganese, 0.15-0.2ppm of iodine, 0.25-0.3ppm of selenium and 0.12-0.15ppm of cobalt; 0.9 percent of macromineral raw material calcium hydrophosphate and 1.1 percent of calcium carbonate; the vitamin complex premix reaches the American NRC recommended standard value.

The complete fermentation complete chicken feed prepared in the embodiment is subjected to Qingyuan partridge chicken breeding test together with the complete fermentation complete chicken feed prepared in the embodiment 5, and the test conditions and the test results are detailed in the embodiment 5.

Example 5

This example illustrates the present invention in detail by taking the example of the 9-15 week old Qingyuan pheasant feed produced by Jiaxing biotechnology limited company of Huangshi city in 2018, 5 months. The initial complete feed formula of the Qingyuan partridge chickens of 9-15 weeks in the embodiment comprises the following raw materials in parts by weight:

energy raw material 75%: comprises 56 percent of corn, 5 percent of palm kernel meal, 5 percent of gordon euryale seed residue, 4 percent of tartary buckwheat residue and 5 percent of rice;

protein raw material 21.2%: comprises 10.8 percent of soybean meal, 2 percent of domestic fish meal, 1.4 percent of imported meat and bone meal and 7 percent of peanut kernel meal;

premix of trace mineral raw materials: 0.5%, in this example, copper methionine, zinc lysine, ferric glycinate, manganese methionine, calcium iodate, selenium yeast and cobalt acetate are used as the raw materials of the trace mineral premix, and each kilogram of the trace mineral premix used in this example contains: 12-13g of copper, 90-100g of zinc, 120g of iron 110-ketone, 18-20g of manganese, 0.3-0.4g of iodine, 0.5-0.6g of selenium and 0.24-0.3g of cobalt;

macromineral raw materials: 2%: 0.95 percent of calcium hydrophosphate and 1.05 percent of calcium carbonate.

The compound vitamin premix comprises the following components: 0.5 percent of the feed additive adopts a vitamin premix which is universal for livestock and poultry, and each kilogram of the feed additive contains the following vitamins: vitamin a 2900 ten thousand IU; vitamin D31000 ten thousand IU; vitamin E1.8 ten thousand IU; vitamin K34 g; vitamin B14 g; vitamin B218 g; vitamin B1240 mg; 1.8g of folic acid; 280mg of biotin; 40g of nicotinoyl phenol; 30g of D-pantothenic acid; vitamin B64 g; water content is not higher than 10%;

salt: 0.25 percent;

enzyme preparation: 0.5 percent, and the phytase activity is more than or equal to 5000U/g; amylase is more than or equal to 100U/g; the neutral protease is more than or equal to 1500U/g; xylanase is more than or equal to 1000U/g; the content of the phytase is 0.2 percent, and the content of amylase, neutral protease and xylanase is 0.3 percent

(2) activating the required strain with 2.5% brown sugar water for 9 hr to obtain viable bacteria with viable count of (10-30) x 10 ⁹ Transferring the stirred full-value powder into an inoculation machine, mixing the activated bacterial liquid with cfu/ml activated bacterial liquid according to the proportion of 400kg of activated bacterial liquid per ton of material, and uniformly stirring;

(3) uniformly laying the inoculated materials on a fermentation bed for aerobic fermentation, wherein the laying thickness is 8-10cm, controlling the fermentation temperature to be 30-35 ℃ after the laying is finished, carrying out aerobic fermentation for 3 days, controlling the moisture of the materials to reach 35% and the temperature of the materials to be close to the ambient temperature, and finishing the aerobic fermentation;

(4) putting the fermented material into a breathing membrane bag, sealing in a heat seal manner, continuing aerobic fermentation of the material in the breathing membrane bag until oxygen in the material is exhausted, and stopping fermentation to obtain the fermented feed; the product indexes of the fully fermented complete feed prepared by the embodiment are as follows: water content less than 35%, viable count 3-5 × 10 ⁸ cfu/g, pH value 5.5-6.0, crude protein more than or equal to 13%, shelf life 150 and 180 days; the contents of trace mineral elements in this example are as follows: 6-6.5ppm of copper, 45-50ppm of zinc, 55-60ppm of iron, 9-10ppm of manganese, 0.15-0.2ppm of iodine, 0.25-0.3ppm of selenium and 0.24-0.3ppm of cobalt; macromineral raw materials, namely 0.95 percent of calcium hydrophosphate and 1.05 percent of calcium carbonate; the vitamin complex premix reaches the American NRC recommended standard value.

Preferably, the activated bacteria solution in the preparation method contains bacillus subtilis (60-150). times.10 ⁸ cfu/ml, containing Bacillus licheniformis (20-80). times.10 ⁸ cfu/ml, containing Bacillus amyloliquefaciens (20-70). times.10 ⁸ cfu/ml。

In order to verify the breeding effect of the fully fermented complete chicken feeds prepared in examples 4 and 5, 600 Qingyuan partridge chickens of 0 week old are selected and subjected to a 105-day feeding test, and the chickens are reasonably divided into A, B groups according to the weight and the development condition, and 300 chickens in each group are fed.

The group a is a control group, the group B is a test group, the group a is fed with the unfermented granular feed of the same formula as in examples 4 and 5, the group B is fed with the fully fermented complete chicken feed prepared in examples 4 and 5 (the feed is prepared according to the preparation method of the example), and the specific test results are as follows:

table three: example 4 and example 5 control test results of the fully fermented complete chicken feed and the conventional complete chicken feed for Qingyuan partridge chickens (high-quality broiler chickens)

As can be seen from the third table: the group A has the advantages that the daily average feed intake of group Qingyuan partridge chickens is 12.997g, the daily gain of group is 3.466kg, the average end weight of group is increased by 380.7kg, the average feed-meat ratio is 3.75, the morbidity is 15%, 18 diseases and deaths occur, and the mortality and culling rate is 6%; the daily average feed intake of Qingyuan pockmarkets in the group B is 14.236Kg, the daily gain is 3.879Kg, the weight is 0.413Kg compared with that of a control group, the average weight is 424.71Kg, the weight is 44.01Kg compared with that of the control group, the average feed conversion ratio is 3.67, the disease rate is saved by 0.08 compared with that of the control group, the disease rate is 3.6 percent, the number of dead chickens is 3, the disease rate is 11.4 percent lower than that of the control group, the number of dead chickens is 15 percent less, and the mortality is 1 percent lower than that of the control group by 5 percent. Through the comparative analysis, the fully fermented full-value high-quality broiler feed produced by the method is used for breeding the remote numbing chickens, the broiler weight is increased quickly, the morbidity and the death and culling rate are low, only few dead chickens exist, and the feed conversion ratio is low; moreover, the chicken coop has no ammonia smell, the chicken manure is shaped, and rare diarrhea or coccidiosis occurs, and chicken flocks are quiet and have no respiratory phenomenon at night. The full-fermentation complete feed prepared by the method has a culture effect obviously superior to that of the traditional unfermented feed when being used for raising Qingyuan partridge chickens.

Example 6

This example illustrates the present invention in detail by taking the example of the feed for helan gray laying hens aged 71-73 weeks produced by haxing biotechnology limited, haylon city, 7 months in 2018. The initial complete feed formula of the hailan gray laying hens of 71-73 weeks old in the embodiment comprises the following raw materials in parts by weight:

energy raw material 71.2%: comprises 56 percent of corn, 5 percent of palm kernel meal, 5 percent of gordon euryale seed residue, 4 percent of tartary buckwheat residue and 5 percent of rice;

protein raw material 22%: comprises 10.8 percent of soybean meal, 2 percent of domestic fish meal, 1.4 percent of imported meat and bone meal and 7 percent of peanut kernel meal;

premix of trace mineral raw materials: 0.5%, in this example, copper methionine, zinc lysine, iron glycinate, manganese methionine, calcium iodate, selenium yeast and cobalt acetate are used as the raw materials of the trace mineral premix, and in the trace mineral premix used in this example, each kilogram of the premix contains: 12-13g of copper, 80-90g of zinc, 110g of iron 100-plus, 19-20g of manganese, 0.3-0.4g of iodine, 0.5-0.6g of selenium and 0.24-0.3g of cobalt;

macromineral raw materials: 2%: 0.95 percent of calcium hydrophosphate and 1.05 percent of calcium carbonate; (the granule calcium in the formula is added into a trough when feeding)

The compound vitamin premix comprises the following components: 0.5 percent of vitamin premix for laying hens, wherein the content of each vitamin is as follows (per kilogram): vitamin a 4400 ten thousand IU; vitamin D31000 ten thousand IU; vitamin E7 ten thousand IU; vitamin K310 g; vitamin B14 g; vitamin B220 g; vitamin B1240 mg; 3g of folic acid; 280mg of biotin; 110g of nicotinoyl phenol; 30g of D-pantothenic acid; vitamin B58 g; water content is not higher than 10%;

salt: 0.25 percent;

enzyme preparation: 0.5 percent, and the phytase activity is more than or equal to 5000U/g; amylase is more than or equal to 100U/g; the neutral protease is more than or equal to 1500U/g; xylanase is more than or equal to 1000/g; the U accounts for 0.2% of phytase, 0.3% of amylase, neutral protease and xylanase

as granulated calcium is required to be added in the laying period of the laying hens, 4% of granulated calcium is additionally added in the formula.

(1) calculating the mass of each raw material according to a production plan, weighing, and respectively crushing the raw materials to be crushed into particles with the particle size of 1.5-3.0mm to ensure that the material structure is fluffy; uniformly mixing the raw materials to be prepared into premix with a carrier to prepare the premix; sequentially feeding the energy raw material, the protein raw material, the macromineral raw material, the salt, the trace mineral premix, the vitamin and the enzyme preparation premix in sequence, and uniformly stirring to obtain complete powder;

(2) activating the required strain with 2.5% brown sugar water for 10 hr to obtain viable bacteria with viable count of (10-30) x 10 ⁹ Transferring the stirred complete powder into a inoculation machine for cfu/ml of activated bacterial liquid, mixing the activated bacterial liquid into the complete powder according to the proportion of 350-400kg of activated bacterial liquid in each ton of material, and uniformly stirring;

(3) uniformly laying the inoculated materials on a fermentation bed for aerobic fermentation, wherein the laying thickness is 8-10cm, controlling the fermentation temperature to be 30-35 ℃ after the laying is finished, carrying out aerobic fermentation for 4 days, ensuring that the moisture of the materials reaches 34% and the temperature of the materials is close to the ambient temperature, and finishing the aerobic fermentation;

(4) putting the fermented material into a breathing membrane bag, sealing in a heat seal manner, continuing aerobic fermentation of the material in the breathing membrane bag until oxygen in the material is exhausted, and stopping fermentation to obtain the fermented feed; the product indexes of the prepared full-fermentation complete feed are as follows: the water content is less than or equal to 35 percent, and the viable count is as follows: 3-5X 10 ⁹ cfu/g, pH: 5.5-6.0, the crude protein is more than or equal to 13 percent, and the quality guarantee period is 150-; the contents of trace mineral elements in this example are as follows: 6-6.5ppm of copper, 40-45ppm of zinc, 50-55ppm of iron, 9.5-10ppm of manganese, 0.3-0.4ppm of iodine, 0.25-0.3ppm of selenium and 0.12-0.15ppm of cobalt; macromineral raw material calcium hydrogen phosphate 0.95%, calcium carbonate 1.05% (without additional added granular calcium); the vitamin complex premix reaches the American NRC recommended standard value.

The activated bacterium liquid in the preparation method contains bacillus subtilis (60-150) multiplied by 10 ⁸ cfu/ml, containing Bacillus licheniformis (20-80). times.10 ⁸ cfu/ml, containing Bacillus amyloliquefaciens (20-70). times.10 ⁸ cfu/ml。

In order to verify the effect of the fully fermented complete feed prepared in the embodiment, 3000 helan gray laying hens of 71-73 weeks old are selected in the embodiment and are averagely divided into A, B groups of 1500, wherein a is a control group, B is a test group, the group a is fed with unfermented feed with the same formula as the embodiment, and the group B is fed with the fully fermented complete feed prepared in the embodiment for 21 days. The specific test results are shown in the following table four:

table four: control experiment results of feeding of full-fermented full-price halan gray laying hen (71-73 weeks old) feed prepared in example 6 and traditional full-price halan gray laying hen feed

From this experimental result, it was found that: after three weeks of the test, the decrease of the laying rate of the test group is slow, the decrease of the laying rate is 0.3 percent from 90.2 percent to 89.9 percent, while the decrease of the laying rate of the control group is large, the decrease of the laying rate is 88.2 percent from 90.3 percent and the decrease of the laying rate is 2.1 percent. The feed intake was 119 g for the control group, which was not fermented, and the feed conversion rate was low, whereas 113 g for the experimental group, which was saved by 0.06 of the control group. The eggs of the experimental group weighed 63.7 grams, which was 1.1 grams compared to 62.6 grams of the control group. The feed-egg ratio of the control group is 2.08: 1, comparative experimental group 1.9: 1 is 0.18 percent higher, and the death and culling rate is 0.5 percent higher than that of 2.3 percent of the experimental group, and moreover, the egg phase, the protein viscosity and the egg yellow color of the experimental group are better than those of the control group. Therefore, the fully fermented complete layer feed is better than the traditional layer feed, but calcium in the fully fermented complete layer feed is additionally added into a feed tank in a granular form and cannot be fermented together with the complete feed.

The above embodiments are merely exemplary and should not be construed as limiting the present invention in any way, and any technical solutions of the present invention can be implemented by any way of changing parameters and the like based on the principle of the present invention as claimed in the claims, and should be construed as falling within the protection scope of the present invention as claimed in the claims.

Claims

1. A30-60 kg lean type fattening pig complete fermentation complete feed is characterized in that: the feed is prepared from the following raw materials in percentage by weight:

(1) energy raw material 75%: comprises 46.6 percent of corn, 5 percent of palm kernel meal, 13 percent of paddy, 5.4 percent of gordon euryale seed residue and 5 percent of tartary buckwheat residue;

(2) protein raw material 21.5%: comprises 14 percent of soybean meal, 4 percent of domestic fish meal and 3.5 percent of dry alfalfa meal;

(3) 0.5 percent of trace mineral raw material premix, wherein the trace mineral raw material premix is prepared by taking copper methionine, zinc lysine, ferric glycinate, manganese methionine, calcium iodate, selenium yeast and cobalt acetate as raw materials, and each kilogram of premix contains: 12-16g of copper, 80-120g of zinc, 160g of iron 100-one, 10-20g of manganese, 0.3-0.5g of iodine, 0.4-0.6g of selenium and 0.24-0.3g of cobalt;

(4) macromineral raw materials: 1.7 percent of calcium hydrophosphate, 0.8 percent of calcium carbonate;

(5) the compound vitamin premix comprises the following components: 0.5 percent, and each kilogram of premix contains the following vitamins: vitamin a 4200 ten thousand IU; vitamin D ₃ 700 ten thousand IU; vitamin E5 ten thousand IU; vitamin K ₃ 5g of the total weight of the feed; vitamin B ₁ 5g of the total weight of the mixture; vitamin B ₂ 18g of a mixture; vitamin B ₆ 1g of a compound; vitamin B ₁₂ 60 mg; 1g of folic acid; 280mg of biotin; 60g of nicotinamide; 40g of D-pantothenic acid; the moisture content is not higher than 10%;

(6) salt: 0.25 percent;

(7) enzyme preparation: 0.5 percent, and the phytase activity is more than or equal to 5000U/g; amylase is more than or equal to 100U/g; the neutral protease is more than or equal to 1500U/g; the xylanase accounts for more than or equal to 1000U/g and comprises the following components in percentage by weight: phytase 0.2%, amylase, neutral protease and xylanase 0.3%;

(8) bacterial agent: 0.05 percent of bacillus amyloliquefaciens, 0.02 percent of bacillus licheniformis and 0.01 percent of bacillus pumilus;

the preparation method of the complete fermentation complete feed for the fattening pigs comprises the following steps:

(1) calculating the mass of each raw material according to a production plan, weighing, and respectively crushing the raw materials to be crushed into particles with the particle size of 0.6-1.0mm to ensure that the material structure is fluffy; uniformly mixing the raw materials to be prepared into premix with a carrier to prepare the premix; sequentially feeding the energy raw material, the protein raw material, the macromineral raw material, the salt, the trace mineral premix, the multivitamin premix and the enzyme preparation, and uniformly stirring to obtain complete powder;

(2) activating the strain with 2.5% brown sugar water for 10 hr to obtain viable bacteria with viable count of (2-3) × 10 ¹⁰ Transferring the stirred full-value powder into an inoculation machine, spraying the activated bacterial liquid into the full-value powder according to the proportion of 380kg of the activated bacterial liquid per ton of the material, and uniformly stirring;

(3) uniformly laying the inoculated materials on a fermentation bed for aerobic fermentation, wherein the laying thickness is 5-8cm, controlling the fermentation temperature to be 30-35 ℃ after the laying is finished, carrying out aerobic fermentation for 3.5 days until the moisture content of the materials reaches 31% and the temperature of the materials is close to the ambient temperature, and finishing the aerobic fermentation;

(4) and (3) putting the fermented material into a breathing membrane bag, sealing in a heat seal manner, continuing aerobic fermentation of the material in the breathing membrane bag until oxygen in the breathing membrane bag is exhausted, and automatically stopping fermentation to obtain the feed.

2. The fully fermented complete feed for 30-60kg of lean type fattening pigs according to claim 1, which is characterized in that: the activated bacterial liquid contains bacillus amyloliquefaciens 5 multiplied by 10 ⁹ -1×10 ¹⁰ cfu/ml, 3X 10 Bacillus licheniformis ⁹ -1.5×10 ¹⁰ cfu/ml, 2X 10 Bacillus pumilus ⁹ -5×10 ⁹ cfu/ml。

3. The fully fermented complete feed for 30-60kg of lean type fattening pigs according to claim 1, which is characterized in that: the fermentation bed adopted in the step (3) is a tunnel type fermentation bed, the distance between the fermentation bed and the bottom of a tunnel is 60cm, the width of the tunnel is 150cm, the length of the tunnel is 600cm, the width of the fermentation bed is 150cm, the length of the fermentation bed is 550cm, a temperature control oxygen supply fan is arranged at one end of the tunnel under the fermentation bed, the depth of a temperature control probe of the temperature control oxygen supply fan inserted into a material is 4cm, and the distance between the temperature control probe and the fan is 350 cm.