CN102726596A - Feather protein peptide feed additive and application thereof - Google Patents
Feather protein peptide feed additive and application thereof Download PDFInfo
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- CN102726596A CN102726596A CN2012102014048A CN201210201404A CN102726596A CN 102726596 A CN102726596 A CN 102726596A CN 2012102014048 A CN2012102014048 A CN 2012102014048A CN 201210201404 A CN201210201404 A CN 201210201404A CN 102726596 A CN102726596 A CN 102726596A
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Abstract
The present invention discloses a feather protein peptide feed additive and an application thereof. The preparation method for the feather protein peptide feed additive comprises: 1, mixing a substrate and water to obtain a solid state fermentation culture medium A, inoculating saccharomyces cerevisiae and saccharomycopsis fibuligera in the solid state fermentation culture medium A, culturing for 28-30 hours at a temperature of 30 DEG C, and drying the resulting culture at a temperature of 35-45 DEG C after culturing to obtain a yeast culture; and 2, mixing avian feather powder and the yeast culture, adding water to the resulting mixed material, uniformly stirring to obtain a solid state fermentation culture medium B, respectively inoculating bnfillus licheniformis and bacillus subtilis in the solid state fermentation culture medium B, and carrying out fermentation culture for 30-45 hours at a temperature of 35-38 DEG C to obtain the feather protein peptide feed additive. The feather protein peptide feed additive of the present invention has the following advantages that: feather protein digestion absorption efficiency can be 90%; the additive contains a variety of bioactive peptides, and provides digestion and growth promotion effects; the additive has natural fermentation flavor and good palatability; and pica of livestock and poultry can be significantly improved.
Description
(1) technical field
The present invention relates to a kind of feather protein peptide feed addictive and preparation thereof and application.
(2) background technology
In recent years, with the intensive develop rapidly that turns to characteristics whole world aquaculture, for huge contribution has been made in human growth in the living standard, but feed protein resource begins to occur serious shortage, has restricted the sustainable development of aquaculture.Yet being rich in the multiple essential amino acid of animal, protein content reaches 75%～90% poultry feather and is not but effectively utilized; The feather that every year can be collected utilization by China reaches 20～300,000 tons; 20,000 tons of less thaies that are used for feed after the processing; Most of feather and animal hair are not used appropriately, and have both wasted resource, have polluted environment again.The unavailable main cause of feather is that the feather meal process technology does not pass a test, and the product digestibility is low, and quality is uneven, causes feeding effect not good.
Utilize the feather keratoprotein, must handle, crack the space structure of keratoprotein, make it become the state that to digest and assimilate the keratin science of carrying out.Processing to feather meal has both at home and abroad had certain research.At present, the main both at home and abroad processing of adopting methods such as high-temperature high-pressure hydrolysis, acid-hydrolysis method, alkali hydrolysis method, enzymatic isolation method, microbial method and extrusion to feather meal.These methods can both improve the digestibility of feather meal to a certain extent, but all exist tangible deficiency, do not pass a test like process technology; The product digestibility is low, and quality is uneven, causes feeding effect not good; Economic benefit is not good, and processing procedure causes secondary pollution etc.
(3) summary of the invention
The object of the invention provides a kind of feather protein peptide feed addictive and application; Adopt many bacterial classifications combined ferment to produce a kind of feather protein peptide feed addictive on feather and yeast culture; Not only having eliminated feather can not be by livestock and poultry digestibility and utilization problem; And containing the various active peptide, keratinase, digestive ferment and somatomedin can substitute animal protein feeds such as fish meal, SDPP fully.
The technical scheme that the present invention adopts is:
A kind of feather protein peptide feed addictive; Said feather protein peptide feed addictive prepares as follows: the preparation of (1) yeast culture: substrate is mixed with water a; Obtain solid-state fermentation culture medium A, again saccharomyces cerevisiae and saccharomycopsis fibuligera are inoculated among the solid-state fermentation culture medium A, cultivate 28 ~ 30h down at 30 ℃; After cultivate finishing with culture in 35 ~ 45 ℃ of dryings, obtain yeast culture; Said substrate is made up of following quality proportion raw material: wheat bran 40 ~ 60%, corn flour 3 ~ 10%, vinasse 15 ~ 20%, bean cake powder 20 ~ 25% and urea 2 ~ 5%; The ratio of said water a and substrate gross mass is 0.5 ~ 1:1; (2) feather protein peptide feed addictive preparation: poultry feather is pulverized, obtained the poultry feather powder, the poultry feather powder is mixed with the said yeast culture of step (1); Add entry b, stir, obtain solid-state fermentation culture medium B; Respectively bacillus licheniformis and bacillus subtilis are seeded among the solid-state fermentation culture medium B, 35 ~ 38 ℃ of fermented and cultured 30 ~ 45h are after fermented and cultured finishes; Tunning is dry, pulverize, obtain described feather protein peptide feed addictive; The mass ratio that feeds intake of said poultry feather powder and yeast culture is 9 ~ 49:1, and the ratio of the addition of described water b and yeast culture and poultry feather powder gross mass is 0.5 ~ 1.2:1.
Further, said saccharomyces cerevisiae of step (1) and saccharomycopsis fibuligera add with the form of mycetocyte suspension respectively.
Further, the addition of the said saccharomyces cerevisiae mycetocyte of step (1) suspension counts 1.2 ~ 3.6 * 10 with viable count
7Cfu/g solid-state fermentation culture medium A dry weight.
Further, the addition of said saccharomycopsis fibuligera mycetocyte suspension counts 2 ~ 5 * 10 with viable count
7Cfu/g solid-state fermentation culture medium A dry weight.
Further, the said poultry feather fineness of powder of step (2) is 20 ~ 40 orders, and moisture is less than 10%.
Further, said bacillus licheniformis of step (2) and bacillus subtilis add with the form of mycetocyte suspension respectively.
Further, the addition of said bacillus licheniformis mycetocyte suspension counts 2 ~ 5 * 10 with viable count
7Cfu/g solid-state fermentation culture medium B dry weight.
Further, the addition of said bacillus subtilis mycetocyte suspension counts 5 ~ 30 * 10 with viable count
7Cfu/g solid-state fermentation culture medium B dry weight.
Further; Said feather protein peptide feed addictive prepares as follows: the preparation of (1) yeast culture: substrate is mixed with water a; Boiling 35min sterilizes under 0.12Mpa pressure, and shelving 35min obtains solid-state fermentation culture medium A again; Again saccharomyces cerevisiae ACCC20042 mycetocyte suspension and saccharomycopsis fibuligera ACCC20015 mycetocyte suspension are inoculated into respectively among the solid-state fermentation culture medium A; Cultivate 28 ~ 30h down at 30 ℃, after cultivation finishes that culture is dry under 35 ~ 45 ℃, obtain yeast culture; Said substrate is made up of following quality proportion raw material: wheat bran 40 ~ 60%, corn flour 3 ~ 10%, vinasse 15 ~ 20%, bean cake powder 20 ~ 25% and urea 2 ~ 5%; The ratio of said water a and substrate gross mass is 0.65 ~ 1:1; The addition of said saccharomyces cerevisiae ACCC20042 mycetocyte suspension counts 1.2 * 10 with viable count
7Cfu/g solid-state fermentation culture medium A dry weight; The addition of said saccharomycopsis fibuligera ACCC20015 mycetocyte suspension counts 2 * 10 with viable count
7Cfu/g solid-state fermentation culture medium A dry weight; (2) preparation of feather protein peptide feed addictive: the poultry feather powder is mixed with mass ratio 11.5 ~ 49:1 with the said yeast culture of step (1), add entry b, stir; With cobalt-60 radiation sterilization under 5kGy; Obtain solid-state fermentation culture medium B, respectively with bacillus licheniformis ACCC01198 mycetocyte suspension and bacillus subtilis ACCC01185 mycetocyte suspension inoculation to solid-state fermentation culture medium B, 35 ~ 38 ℃ of fermented and cultured 30 ~ 45h; After fermented and cultured finishes; Tunning is dried to moisture less than 10% under 45 ℃, being crushed to fineness is 40 ~ 60 orders, obtains described feather protein peptide feed addictive; Said poultry feather powder moisture is less than 10%, and fineness is 20 ~ 40 orders; The ratio of said water b and yeast culture and poultry feather powder gross mass is 0.5 ~ 1.2:1; The addition of said bacillus licheniformis mycetocyte suspension counts 2 ~ 5 * 10 with viable count
7Cfu/g solid-state fermentation culture medium B dry weight; The addition of said bacillus subtilis mycetocyte suspension counts 5 ~ 8 * 10 with viable count
7Cfu/g solid-state fermentation culture medium B dry weight.
The application of a kind of said feather protein peptide feed addictive in the livestock and poultry mixed feed, further, the effective mass addition of said feather protein peptide feed addictive in the livestock and poultry mixed feed is 2 ~ 8% (weight).
Solid-state fermentation culture medium dry weight according to the invention is meant dries the solid-state fermentation culture medium of preparation to constant weight at 55 ~ 58 ℃, measures the water content of the solid-state fermentation culture medium that every restraint is equipped with, and calculates the dry weight of prepared solid-state fermentation culture medium according to water content.
Poultry feather powder according to the invention comes from the feather of various birds, the feather of preferred following bird: chicken, duck, goose or quail etc.
Saccharomyces cerevisiae according to the invention; Be preferably saccharomyces cerevisiae (Saccharomyces cerevisiaeACCC20042), available from Chinese agriculture microorganism fungus kind preservation administrative center, said saccharomyces cerevisiae ACCC20042 mycetocyte suspension prepares as follows: saccharomyces cerevisiae ACCC20042 is seeded in the maltose liquid glucose a seed culture medium (7～8 ° of Be of pol); 30 ℃ of heat insulating culture 10h; Be forwarded in the maltose liquid glucose b seed culture medium (7～8 ° of Be of pol), 30 ℃ of heat insulating culture 10h are forwarded in the syrup culture medium (7～8 ° of Be of pol) then again; Cultivate 10h for 30 ℃; Be forwarded to 30 ℃ of heat insulating culture 20h of enlarged culture base (consist of: the mass ratio of wheat bran and flour and water is 1:3:20) at last, obtain the saccharomyces cerevisiae seed liquor, be saccharomyces cerevisiae mycetocyte suspension; The volume ratio of said maltose liquid glucose a and maltose liquid glucose b is 1:6, and said maltose liquid glucose a and maltose liquid glucose b are the maltose liquid glucose, and letter itself does not have implication; Flow process is: the former bacterium test tube of saccharomyces cerevisiae---little triangular flask seed (maltose liquid glucose 100ml; 7～8 ° of Be of pol) 30 ℃ of heat insulating culture 10h---change big triangular flask (maltose liquid glucose 600ml over to; 7～8 ° of Be of pol); 30 ℃ of heat insulating culture 10h---change (syrup 13kg, 7～8 ° of Be enlarged culture of pol) 30 ℃ of cultivations 10h in the Ka Shi jar over to---, and Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg, flour 30kg; Wheat bran 10kg) total incubation time 20h is the saccharomyces cerevisiae seed liquor.
Saccharomycopsis fibuligera according to the invention is preferably saccharomycopsis fibuligera (Endomycopsisfibuligera ACCC20015), available from Chinese agriculture microorganism fungus kind preservation administrative center.Said saccharomycopsis fibuligera ACCC20015 mycetocyte suspension preparation method is identical with said saccharomyces cerevisiae ACCC20042 mycetocyte suspension preparation method; Flow process is: the former bacterium test tube of saccharomycopsis fibuligera---little triangular flask seed (maltose liquid glucose 100ml; 7～8 ° of Be of pol) 30 ℃ of heat insulating culture 10h---change big triangular flask (maltose liquid glucose 600ml over to; 7～8 ° of Be of pol); 30 ℃ of heat insulating culture 10h---change (syrup 13kg, 7～8 ° of Be enlarged culture of pol) 30 ℃ of cultivations 10h in the Ka Shi jar over to---, and Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg, flour 30kg; Wheat bran 10kg) total incubation time 20h is the saccharomycopsis fibuligera seed liquor.
Bacillus licheniformis according to the invention (Bacillus licheniformis); Preferred bacillus licheniformis ACCC01198 (Bacillus licheniformis); Available from Chinese agriculture microorganism fungus kind preservation administrative center, said bacillus licheniformis mycetocyte suspension prepares as follows: bacillus licheniformis is seeded in the liquid seed culture medium that is applicable to the bacillus licheniformis growth, puts on the shaking table then; Rotating speed is 240r/min; 40 ℃ of shaken cultivation 14h obtain the bacillus licheniformis seed liquor, are bacillus licheniformis mycetocyte suspension; Further; Said bacillus licheniformis ACCC01198 mycetocyte suspension prepares as follows: (1) inclined-plane is cultivated: the slant medium final concentration consists of: beef extract 5g/L, peptone 10g/L, sodium chloride 5g/L, agar 30g/L; PH value 7.4, solvent are water; Bacillus licheniformis ACCC01198 is transferred in fresh test tube slant from the inclined-plane of preserving,, obtain slant strains in 37 ℃ of cultivation 18h; (2) seed culture: the liquid seed culture medium final concentration consists of: beef extract 5g/L, and soy peptone 10g/L, NaCl 5g/L, pH7.0, solvent are water; From the cultured inclined-plane of step (1), choosing 1 ring with oese is inoculated in the 250ml triangular flask that fills 50ml sterile liquid seed culture medium; Put the speed governing of swinging constant temperature then and shake 37 ℃ of vibrations (rotating speed is 120r/min) cultivation 24h in bottle cabinet; Obtain bacillus licheniformis ACCC01198 seed liquor, i.e. bacillus licheniformis ACCC01198 mycetocyte suspension.
Bacillus subtilis according to the invention is preferably bacillus subtilis ACCC01185 (Bacillus subtilis), available from Chinese agriculture microorganism fungus kind preservation administrative center.Said bacillus subtilis mycetocyte suspension prepares as follows: bacillus subtilis is seeded in the liquid seed culture medium that is applicable to the bacillus subtilis bacteria growing; 35～37 ℃, 220r/min shaking table were cultivated 24～26 hours; Obtain the bacillus subtilis seed liquor, i.e. bacillus subtilis mycetocyte suspension; Further; Said bacillus subtilis ACCC01185 mycetocyte suspension prepares as follows: (1) inclined-plane is cultivated: the slant medium final concentration consists of: peptone 10g/L, beef extract 3g/L, NaC1 5g/L, agar 20g/L, pH value 7.0, solvent are water; Bacillus subtilis ACCC01185 is transferred in fresh test tube slant from the inclined-plane of preserving, cultivate 24h, obtain slant strains for 37 ℃; (2) seed culture: the liquid seed culture medium final concentration consists of: glucose 2g/L, and NaC1 5g/L, yeast extract 5g/L, peptone l0g/L, pH 7.0, and solvent is a water; With the liquid seed culture medium 30min that under 120 ℃ of conditions, sterilizes; The cooling back is chosen 1 ring with oese and is inoculated in the 300ml triangular flask that fills 50ml sterile liquid seed culture medium from the cultured inclined-plane of step (1); Put 37 ℃ of temperature control shaking tables and cultivate, rotating speed 220r/min, reaching to the gemma rate (needs 24h approximately) more than 90% time stops; Obtain bacillus subtilis ACCC01185 seed liquor, be bacillus subtilis ACCC01185 mycetocyte suspension.
Solid-state fermentation culture medium A according to the invention, solid-state fermentation culture medium B are solid-state fermentation culture medium, water a, and water b is water, and letter itself does not have implication.
Beneficial effect of the present invention is mainly reflected in: feather protein peptide feed addictive of the present invention can make feather protein digest and assimilate efficient to reach 90%; Contain multiple biologically active peptide; And through the multiple beneficial microbial fermentation; Form through low temperature drying, be rich in keratinase, digestive ferment, active bacterium, have aid digestion and promote and grow; Help regulating and control the animal intestinal beneficial microbe colony; Have natural fermenting aroma, good palatability has good food calling effect, promotes young animal to search for food; Can obviously improve the allotriophagy of livestock and poultry, can substitute animal protein feeds such as fish meal, SDPP fully, have tangible economic benefit and social benefit.
(4) specific embodiment
Below in conjunction with specific embodiment the present invention is described further, but protection scope of the present invention is not limited in this:
The bent preparation of the kind of embodiment 1 bacterial classification
(1) bacillus subtilis (Bacillus subtilis ACCC01185, Chinese agriculture microorganism fungus kind preservation administrative center provides):
The slant medium final concentration consists of: peptone 1g, beef extract 0.3g, NaC10.5g, agar 2.0g, distilled water 100ml, pH value 7.0.
Liquid seed culture medium is: glucose 0.2g, and NaC10.5g, yeast extract 0.5g, peptone lg, distilled water 100mL, pH 7.0.30min sterilizes under 120 ℃ of conditions.
ACCC01185 is inoculated in slant medium with bacillus subtilis, cultivates 24h, obtains slant strains for 37 ℃; From slant strains picking one oese in the 300ml triangular flask that fills 50ml sterile liquid seed culture medium; Putting 37 ℃ of temperature control shaking tables cultivates; Rotating speed 220r/min; Reaching to the gemma rate (needs 24h approximately) more than 90% time stops, and obtains bacillus subtilis ACCC01185 seed liquor, is bacillus subtilis ACCC01185 mycetocyte suspension.
(2) bacillus licheniformis (Bacillus licheniformis ACCC01198 is provided by Chinese agriculture microorganism fungus kind preservation administrative center):
The slant medium final concentration consists of: beef extract 5g, peptone 10g, sodium chloride 5g, agar 30g, distilled water 1000mL, pH value 7.4.
Liquid seed culture medium is: beef extract 5g, soy peptone 10g, NaC15g, distilled water 1L, pH7.0.
Bacillus licheniformis is transferred in fresh test tube slant from the inclined-plane of preserving,, obtain slant strains in 37 ℃ of cultivation 18h; Get cultured slant strains; Choosing 1 ring with oese is inoculated in the 250ml triangular flask that fills 50ml aseptic seed culture medium; Put the speed governing of swinging constant temperature then and shake 37 ℃ of vibrations (rotating speed is 120r/min) cultivation 24h in bottle cabinet; Obtain bacillus licheniformis ACCC01198 seed liquor, be bacillus licheniformis ACCC01198 mycetocyte suspension.
(3) saccharomyces cerevisiae (Saccharomyces cerevisiae ACCC20042, Chinese agriculture microorganism fungus kind preservation administrative center provides):
The former bacterium test tube of saccharomyces cerevisiae---little triangular flask seed (maltose liquid glucose 100ml; 7～8 ° of Be of pol) 30 ℃ of heat insulating culture 10h---change big triangular flask (maltose liquid glucose 600ml over to; 7～8 ° of Be of pol); 30 ℃ of heat insulating culture 10h---change (syrup 13kg, 7～8 ° of Be enlarged culture of pol) 30 ℃ of cultivations 10h in the Ka Shi jar over to---, and Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg, flour 30kg; Wheat bran 10kg) total incubation time 20h is the saccharomyces cerevisiae seed liquor.
(4) saccharomycopsis fibuligera (Endomycopsisfbuligera ACCC20015, administrative center provides by the preservation of Chinese agriculture microorganism fungus kind):
The former bacterium test tube of saccharomycopsis fibuligera---little triangular flask seed (maltose liquid glucose 100ml; 7～8 ° of Be of pol) 30 ℃ of heat insulating culture 10h---change big triangular flask (maltose liquid glucose 600ml over to; 7～8 ° of Be of pol); 30 ℃ of heat insulating culture 10h---change (syrup 13kg, 7～8 ° of Be enlarged culture of pol) 30 ℃ of cultivations 10h in the Ka Shi jar over to---, and Ka Shi jar yeast starter is spread cultivation at the distiller's yeast cylinder, and (culture medium is that every cylinder is put clear water 200kg, flour 30kg; Wheat bran 10kg) total incubation time 20h is the saccharomycopsis fibuligera seed liquor.
Embodiment 2 feather protein peptide feed addictives
(1) preparation of yeast culture:
Described yeast culture preparation method: will be by wheat bran 600g, corn flour 30g, vinasse 150g; Bean cake powder 200g, the fermentation substrate that urea 20g forms adds in the sterilization tank, adds 650g water again; Boiling 35min sterilizes under 0.12Mpa pressure; Close inlet valve after the sterilization, shelving 35min discharging obtains solid-state fermentation culture medium.Ventilation blast-cold to 33～35 ℃, (viable count reaches 1.2 * 10 with the solid-state fermentation culture medium dry weight basis to inoculate the saccharomyces cerevisiae ACCC20042 mycetocyte suspension 40g of embodiment 1 method preparation respectively
7Cfu/g), (viable count reaches 2 * 10 with the solid-state fermentation culture medium dry weight basis to the multiple film spore ferment ACCC20015 mycetocyte suspension 50g of button capsule
7Cfu/g), fermentation temperature was controlled at 30 ℃ of fermented and cultured after 30 hours, was dried to moisture less than 10% at 35 ℃, processed yeast culture.
(2) preparation of feather protein peptide feed addictive
The feather powder (all can by various poultry feathers; Wash clean; Dry to moisture less than 10%, being crushed to fineness is 40 orders) after the yeast culture 80g of 920g and step (1) preparation mixes, add 500g water; Under 5kGy, carry out irradiation sterilization with cobalt-60, obtain the feather protein peptide solid-state fermentation culture medium.(viable count reaches 2 * 10 with the solid-state fermentation culture medium dry weight basis to the bacillus licheniformis ACCC01198 mycetocyte suspension 1g that respectively embodiment 1 method is prepared
7Cfu/g), (viable count reaches 5 * 10 with the solid-state fermentation culture medium dry weight basis to bacillus subtilis ACCC01185 mycetocyte suspension 3g
7Cfu/g), in 35-38 ℃ of following blend fermented and cultured.Fermentation temperature has mainly been managed in fermentation.Open temperature-controlled instrument (BYS-II mark is supported the wet auto-controlling apparatus of room temperature, Hebei province rainbow space instrument and equipment Co., Ltd), connect fan power, the temperature upper control limit is 38 ℃, is limited to 35 ℃ down.The diligent observation, diligent test.Temperature controller probe drop point wants correct, and the degree of depth will reach 10cm (doing contrast but plug in thermometer on the limit), mainly prevents probe; Temperature controller and connection instrument fault; Begin behind the general 6h to heat up, wind is touring in the early stage used chamber, when room temperature higher (reaching 36 ℃ usually) or expect that the temperature rise temperature is too quickly; But air door outside the opening chamber reaches the purpose of regulating growth temperature.Finish through the 30h fermentation, can strengthen air door, door and window makes the material temperature be lower than 40 ℃.Adopt low temperature drying, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.Be crushed to fineness 40 orders after the oven dry, obtain moisture≤10%, crude protein quality content>=60% feather protein peptide feed addictive.
Embodiment 3 feather protein peptide feed addictives
(1) yeast culture preparation
Described yeast culture preparation method: will be by wheat bran 400g, corn flour 100g, vinasse 200g; Bean cake powder 250g, the fermentation substrate that urea 50g forms add in the sterilization tank, add 1000g water; Boiling 35min sterilizes under 0.12Mpa pressure; Close inlet valve after the sterilization, shelving 35min discharging obtains solid-state fermentation culture medium.Ventilation blast-cold to 33～35 ℃, (viable count reaches 3.6 * 10 with the solid-state fermentation culture medium dry weight basis to inoculate the saccharomyces cerevisiae ACCC20042 mycetocyte suspension 120g of embodiment 1 method preparation respectively
7Cfu/g), (viable count reaches 5 * 10 with the solid-state fermentation culture medium dry weight basis to the multiple film spore ferment ACCC20015 mycetocyte suspension 125g of button capsule
7Cfu/g) fermentation temperature is controlled at 30 ℃, and fermented and cultured was dried to moisture less than 10% at 35 ℃ ~ 45 ℃ after 28 hours altogether, processed yeast culture.
(2) preparation of feather protein peptide feed addictive
Feather meal (various poultry feathers all can, wash clean, dry to moisture less than 10%; Being crushed to fineness is 20 orders) after the yeast culture 20g of 980g and step (1) preparation mixes; Add 1200g water, under 5kGy, carry out irradiation sterilization, obtain the feather protein peptide solid-state fermentation culture medium with cobalt-60.(viable count reaches 5 * 10 with the solid-state fermentation culture medium dry weight basis to the bacillus licheniformis ACCC01198 mycetocyte suspension 2.5g that respectively embodiment 1 method is prepared
7Cfu/g), (viable count reaches 3 * 10 with the solid-state fermentation culture medium dry weight basis to bacillus subtilis ACCC01185 mycetocyte suspension 18g
8Cfu/g), in 35 ~ 38 ℃ of following blend fermented and cultured.Other operations finish through the 45h fermentation with embodiment 2, can strengthen air door, and door and window does not make the material temperature surpass 40 ℃, waits for out the room drying.Adopt low temperature drying, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.Be crushed to after the oven dry more than fineness 60 orders, obtain moisture≤10%,, crude protein>=60% feather protein peptide feed addictive.
Embodiment 4 feather protein peptide feed addictives
(1) preparation of yeast culture:
Described yeast culture preparation method: will be by wheat bran 500g, corn flour 65g, vinasse 175g; Bean cake powder 225g, the fermentation substrate that urea 35g forms adds in the sterilization tank, adds 825g water again; Boiling 35min sterilizes under 0.12Mpa pressure; Close inlet valve after the sterilization, shelving 35min discharging obtains solid-state fermentation culture medium.Ventilation blast-cold to 33～35 ℃, (viable count reaches 2.4 * 10 with the solid-state fermentation culture medium dry weight basis to inoculate the saccharomyces cerevisiae ACCC20042 mycetocyte suspension 80g of embodiment 1 method preparation respectively
7Cfu/g), (viable count reaches 3.5 * 10 with the solid-state fermentation culture medium dry weight basis to the multiple film spore ferment ACCC20015 mycetocyte suspension 87.5g of button capsule
7Cfu/g), fermentation temperature was controlled at 30 ℃ of fermented and cultured after 28 hours, was dried to moisture less than 10% at 35 ℃, processed yeast culture.
(2) preparation of feather protein peptide feed addictive
Feather meal (various poultry feathers all can, wash clean, dry to moisture less than 10%; Being crushed to fineness is 35 orders) after the yeast culture 50g of 950g and step (1) preparation mixes; Add 850g water, carry out irradiation sterilization at 5kGy, obtain the feather protein peptide solid-state fermentation culture medium with cobalt-60.(viable count reaches 3.5 * 10 with the solid-state fermentation culture medium dry weight basis to the bacillus licheniformis ACCC01198 mycetocyte suspension 1.75g that respectively embodiment 1 method is prepared
7Cfu/g), (viable count reaches 1.75 * 10 with the solid-state fermentation culture medium dry weight basis to bacillus subtilis ACCC01185 mycetocyte suspension 10.5g
8Cfu/g), in 35-38 ℃ of following blend fermented and cultured.Other operations finish through the 45h fermentation with embodiment 2, can strengthen air door, and door and window does not make the material temperature surpass 40 ℃, waits for out the room drying.Adopt low temperature drying, expect temperature control early stage in 38 ℃, and later stage material temperature control is in 45 ℃, and moisture can go out the room less than 10%.Be crushed to after the oven dry more than fineness 60 orders, obtain moisture≤10%, crude protein quality content>=60% feather protein peptide feed addictive.
Embodiment 5
Choose 108 of the three way cross piglets of 28 ages in days wean, by nest not, sex, principle that body weight is close be divided into 3 groups (test I group, test II group and control groups), every group of 3 repetitions, each repeats l2 pig.Control group fed basal diet (the quality prescription is seen table 1); Test I group, test II group use the feather protein peptide feed addictive of the embodiment 2 methods preparation of 2% (weight) and 5% (weight) to substitute the fish meal of basal diet moderate respectively; Experimental period 28-49 age in days; Free choice feeding, production performance result sees shown in the table 2.
Table 1 daily ration mass component is formed
Compound premix is to contain in every kilogram of diet: iron 130mg, copper 200mg, zinc 130mg, manganese 40mg, iodine 0.35mg, selenium 0.30mg, vitamin A 15750IU, vitamin D 3500IU, vitamin E 28IU, vitamin K 3.5mg, thiamine 3.5mg, riboflavin 8.75mg, nicotinic acid 35mg, pantothenic acid 17.5mg, Cobastab
64.2mg, folic acid 1.75mg, biotin 175g, choline 300mg.
The feather protein peptide feed addictive is seen table 2 to the influence of weanling pig growth performance.Can obviously improve daily gain, reduce the material anharmonic ratio with 2% (test I group) and 5% (test II group) feather protein peptide feed addictive equivalent substitution fish meal.
Table 2 feather protein peptide feed addictive influences the weanling pig growth performance
Annotate: with the adjacent lowercase alphabet differential of line data shoulder mark different significantly (P < O.05), the capitalization person of being separated by representes difference extremely significantly (P < 0.01).Following table together
Embodiment 6
Choose 60 of healthy three way cross Du * big * long 28 age in days weanling pigs, 6 nests, according to nest not, litter size, initial body heavy phase unanimity be divided into 2 groups at random: control group and test group, 3 every group repetitions (the piglet number of each repetition is the 8-11 head).Control group is fed daily ration, and the daily ration prescription is seen table 3.Test group substitutes 4% (weight) fish meal and 4% (weight) dregs of beans in the control group daily ration with the feather protein peptide feed addictive of 8% (weight) embodiment, 3 methods preparation; Free choice feeding; The 28-30 age in days is preliminary trial period; The 30-53 age in days formal test phase, formal test phase piglet growth situation is seen shown in the table 4.
Table 3 test daily ration quality is formed
Premix is that every kilogram of diet contains: iron 130mg, copper 200mg, zinc 130mg, manganese 40mg, iodine 0.35mg, selenium 0.30mg, vitamin A 15750IU, cholecalciferol 500IU, vitamin E 28IU, vitamin K 3.5mg, thiamine 3.5mg, riboflavin 8.75mg, nicotinic acid 35mg, pantothenic acid 17.5mg, Cobastab
64.2mg, folic acid 1.75mg, biotin 175g, choline 300mg.
Table 4 shows, substitutes 4% fish meal and 4% dregs of beans in the control group daily ration with 8% feather protein peptide feed addictive, and weanling pig growth performance effect has clear improvement.
Table 4 feather protein peptide feed addictive is to the influence of weanling pig growth performance
Annotate: with the adjacent lowercase alphabet differential of line data shoulder mark different significantly (P < O.05), the capitalization person of being separated by representes difference extremely significantly (P < 0.01).
Embodiment 7
Select 1 age in days Ai Wei mattress fryer, 360 plumages, be divided into 4 groups, be respectively control group, test group I, test group II, test group III by the feeding experiment requirement; Each organizes 3 repetitions, and each repeats 30 plumage chickens.Control group is fed basal diet (the basal diet prescription is seen shown in the table 5); Test I, test II group use the feather protein peptide feed addictive of the embodiment 4 methods preparation of 2% (weight) and 5% (weight) to substitute the fish meal of basal diet moderate respectively, and the test group III substitutes 5% (weight) fish meal and 3% (weight) dregs of beans in the basal diet with the feather protein peptide feed addictive of 8% (weight).The feeding process free choice feeding, the feather protein peptide feed addictive is seen shown in the table 6 growth of meat chicken Effect on Performance result.
The quality prescription of table 5 test daily ration
Compound premix is that every kilogram of daily ration provides in the table 5: vitamin A 12500IU, cholecalciferol 4125IU, vitamin e1 5IU, vitamin K 2mg, thiamine 1mg; Riboflavin 8.5mg, calcium pantothenate 50mg, nicotinic acid 32.5mg, pyridoxol 8mg, biotin 2mg; Folic acid 5mg, Cobastab-5mg, choline 500mg, manganese 65mg, iodine 1mg; Iron 60mg, copper 8mg, zinc 66nag, selenium 0.3mg.
Table 6 shows that the test group of using the feather protein peptide feed addictive can obviously improve fryer 42 age in days body weight, average daily gain, reduction material anharmonic ratio.
Table 6 feather protein peptide feed addictive is to the growth of meat chicken Effect on Performance
Annotate: with the adjacent lowercase alphabet differential of line data shoulder mark different significantly (P < O.05), the capitalization person of being separated by representes difference extremely significantly (P < 0.01).
Claims (10)
1. feather protein peptide feed addictive; It is characterized in that said feather protein peptide feed addictive prepares as follows: the preparation of (1) yeast culture: substrate is mixed with water a; Obtain solid-state fermentation culture medium A, again saccharomyces cerevisiae and saccharomycopsis fibuligera are inoculated among the solid-state fermentation culture medium A, cultivate 28 ~ 30h down at 30 ℃; After cultivate finishing with culture in 35 ~ 45 ℃ of dryings, obtain yeast culture; Said substrate is made up of following quality proportion raw material: wheat bran 40 ~ 60%, corn flour 3 ~ 10%, vinasse 15 ~ 20%, bean cake powder 20 ~ 25% and urea 2 ~ 5%; Said water a and substrate total mass ratio are 0.5 ~ 1:1; (2) preparation of feather protein peptide feed addictive: poultry feather is pulverized, obtained the poultry feather powder, the poultry feather powder is mixed with the said yeast culture of step (1); Add entry b, stir, obtain solid-state fermentation culture medium B; Respectively bacillus licheniformis and bacillus subtilis are seeded among the solid-state fermentation culture medium B, 35 ~ 38 ℃ of fermented and cultured 30 ~ 45h are after fermented and cultured finishes; Tunning is dry, pulverize, obtain described feather protein peptide feed addictive; The mass ratio that feeds intake of said poultry feather powder and yeast culture is 9 ~ 49:1, and the addition of described water b and yeast culture and poultry feather powder gross mass ratio are 0.5 ~ 1.2:1.
2. feather protein peptide feed addictive according to claim 1 is characterized in that said saccharomyces cerevisiae of step (1) and saccharomycopsis fibuligera add with the form of mycetocyte suspension respectively.
3. like the said feather protein peptide feed addictive of claim 2, it is characterized in that the addition of the said saccharomyces cerevisiae mycetocyte of step (1) suspension counts 1.2 ~ 3.6 * 10 with viable count
7Cfu/g solid-state fermentation culture medium A dry weight.
4. like the said feather protein peptide feed addictive of claim 2, it is characterized in that the addition of the said saccharomycopsis fibuligera mycetocyte of step (1) suspension counts 2 ~ 5 * 10 with viable count
7Cfu/g solid-state fermentation culture medium A dry weight.
5. feather protein peptide feed addictive according to claim 1 is characterized in that the said poultry feather powder of step (2) moisture less than 10%, and fineness is 20 ~ 40 orders.
6. feather protein peptide feed addictive according to claim 1 is characterized in that said bacillus licheniformis of step (2) and bacillus subtilis add with the form of mycetocyte suspension respectively.
7. like the said feather protein peptide feed addictive of claim 6, it is characterized in that the addition of said bacillus licheniformis mycetocyte suspension counts 2 ~ 5 * 10 with viable count
7Cfu/g solid-state fermentation culture medium B dry weight.
8. like the said feather protein peptide feed addictive of claim 6, it is characterized in that the addition of said bacillus subtilis mycetocyte suspension counts 5 ~ 30 * 10 with viable count
7Cfu/g solid-state fermentation culture medium B dry weight.
9. feather protein peptide feed addictive according to claim 1; It is characterized in that said feather protein peptide feed prepares as follows: the preparation of (1) yeast culture: substrate is mixed with water a; Boiling 35min sterilizes under 0.12MPa pressure, and shelving 35min obtains solid-state fermentation culture medium A again; Again saccharomyces cerevisiae ACCC20042 mycetocyte suspension and saccharomycopsis fibuligera ACCC20015 mycetocyte suspension are inoculated into respectively among the solid-state fermentation culture medium A; Cultivate 28 ~ 30h down at 30 ℃, after cultivation finishes that culture is dry under 35 ~ 45 ℃, obtain yeast culture; Said substrate is made up of following quality proportion raw material: wheat bran 40 ~ 60%, corn flour 3 ~ 10%, vinasse 15 ~ 20%, bean cake powder 20 ~ 25% and urea 2 ~ 5%; The ratio of said water a and substrate gross mass is 0.65 ~ 1:1; The addition of said saccharomyces cerevisiae ACCC20042 mycetocyte suspension counts 1.2 * 10 with viable count
7Cfu/g solid-state fermentation culture medium A dry weight; The addition of said saccharomycopsis fibuligera ACCC20015 mycetocyte suspension counts 2 * 10 with viable count
7Cfu/g solid-state fermentation culture medium A dry weight; (2) preparation of feather protein peptide feed addictive: the poultry feather powder is mixed with mass ratio 11.5 ~ 49:1 with the said yeast culture of step (1), add entry b, stir; With cobalt-60 radiation sterilization under 5kGy; Obtain solid-state fermentation culture medium B, respectively with bacillus licheniformis ACCC01198 mycetocyte suspension and bacillus subtilis ACCC01185 mycetocyte suspension inoculation to solid-state fermentation culture medium B, 35 ~ 38 ℃ of fermented and cultured 30 ~ 45h; After fermented and cultured finishes; Tunning is dried to moisture less than 10% under 45 ℃, being crushed to fineness is 40 ~ 60 orders, obtains described feather protein peptide feed addictive; Said poultry feather powder moisture is less than 10%, and fineness is 20 ~ 40 orders; The ratio of said water b and yeast culture and poultry feather powder gross mass is 0.5 ~ 1.2:1; The addition of said bacillus licheniformis mycetocyte suspension counts 2 ~ 5 * 10 with viable count
7Cfu/g solid-state fermentation culture medium B dry weight; The addition of said bacillus subtilis mycetocyte suspension counts 5 ~ 8 * 10 with viable count
7Cfu/g solid-state fermentation culture medium B dry weight.
10. the application of feather protein peptide feed addictive in the livestock and poultry mixed feed according to claim 1 is characterized in that the effective mass addition of said feather protein peptide feed addictive in the livestock and poultry mixed feed is 2 ~ 8%.
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| CN103053833A (en) * | 2012-12-20 | 2013-04-24 | 福建省农业科学院中心实验室 | Philippines eel feed containing feather peptide powder |
| CN103947850A (en) * | 2014-03-13 | 2014-07-30 | 杭州启明星生物科技研究院有限公司 | Allotriphagia conditioning agent for poultry and application thereof |
| CN105454658A (en) * | 2015-12-21 | 2016-04-06 | 合肥工业大学 | Preparation method of feather fermentation protein feed |
| CN106071104A (en) * | 2016-06-14 | 2016-11-09 | 杭州百瑞特饲料科技有限公司 | A kind of expanded fermentation protein feedstuff additive and application |
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| CN115067435A (en) * | 2022-06-24 | 2022-09-20 | 广东省科学院生物与医学工程研究所 | High-protein pigeon feed taking waste feathers, pericarps and schizochytrium limacinum fungus residues as raw materials and preparation method thereof |
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| CN105454658A (en) * | 2015-12-21 | 2016-04-06 | 合肥工业大学 | Preparation method of feather fermentation protein feed |
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| CN106689692A (en) * | 2016-11-25 | 2017-05-24 | 彭程 | Protein feed for substituting soybean meal or fish meal |
| CN107594090A (en) * | 2017-10-30 | 2018-01-19 | 广州昆虫蛋白生物科技有限公司 | A kind of preparation method of insect microbial cell broken wall fermentation protein feedstuff |
| CN109287855A (en) * | 2018-09-28 | 2019-02-01 | 青岛农业大学 | A kind of fermentation process of corn stover, the fermented maize stalk of this method preparation and application |
| CN112655825A (en) * | 2020-12-23 | 2021-04-16 | 安徽希普生物科技有限公司 | Feather protein peptide particle composition and preparation method thereof |
| CN115067435A (en) * | 2022-06-24 | 2022-09-20 | 广东省科学院生物与医学工程研究所 | High-protein pigeon feed taking waste feathers, pericarps and schizochytrium limacinum fungus residues as raw materials and preparation method thereof |
| CN115606678A (en) * | 2022-10-08 | 2023-01-17 | 广东省科学院生物与医学工程研究所 | Method for preparing high-protein probiotic animal feed by using waste feathers through composite feeding probiotics |
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