CN101240302A - Method for producing L-phenylalanine by using bacillus subtilis to ferment feather - Google Patents
Method for producing L-phenylalanine by using bacillus subtilis to ferment feather Download PDFInfo
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Abstract
The invention discloses a method for preparing L-phenylalanine utilizing bacillus subtilis fermentation feather comprising steps mixing abandoning feather, common salt, magnesium chloride, calcii chloridum, patassium dihydrogen phosphate, dibasic potassium phosphate and yeast powder in proportion, solving by purified water and sterilizing by heat treating, then transferring bacillus subtilis YYW-1 CGMCC NO.2396, fermenting at 35-40 DEG C for 48-72 hours, acid-conditioning the fermentation outcome, absorbing and cleaning by 732 ammonium -type cationic ion-exchange resin to obtain L-phenylalanine concentrated solution. In the method, the raw material cost is low and the wasted is utilized in high value, furthermore, the prepared L-phenylalanine has high optical purity and good quality and can satisfy the need of industry and medicine industry.
Description
Technical field
The present invention relates to a kind of preparation method of phenylalanine, relate in particular to a kind of method of utilizing subtilis (Bacillussubtilis) ferment feather to produce the L-phenylalanine, belong to the amino acid fermentation preparation field.
Background technology
The L-phenylalanine has another name called L-phenyl-α-An Jibingsuan, English name L-Phenylalanine, and molecular formula is C
9H
11NO
2, molecular weight is 165.19, white crystalline powder, and special odor slightly, bitter, 283 ℃ of fusing points (decomposition), solubleness is 3% in the water, is slightly soluble in ethanol, is insoluble to ether.Phenylalanine has racemize DL-type, L-type and D-type, wherein the most important thing is the L-phenylalanine, mainly is present in ovum, breast and the animal proteinum at occurring in nature, and content 5%-6% contains 1% in the vegetable protein approximately.
The L-phenylalanine is one of 8 kinds of indispensable amino acids of needed by human body, and humans and animals self can not synthesize, and must absorb from the external world, also is the important source material of synthetic hydroxyphenylaminopropionic acid in the organism, has vital role in the metabolic process of human body.Refining pharmacopeia level L-phenylalanine can be used for preparing the aminoacids complex transfusion, also can be used for amino acids cancer therapy drug, dietary supplements Application and Development, and China's pharmaceutical industries is annual at present need consume about 500 tons of L-phenylalanines.
The topmost purposes of L-phenylalanine is synthetic sweetener aspartame (Aspartame is called for short APM).Aspartame claims aspartame, protein sugar again; chemistry N-L-α-aspartyl by name-L-phenylalanine-1-formicester is a kind of by L-aspartic acid and L-phenylalanine (nutritive substance that human body is necessary) synthetic dipeptides, and metabolism fully can be absorbed by the body; nontoxic; safe and reliable, the refrigerant tasty and refreshing sucrose that exactly likes of pure taste, but sugariness is 200 times of sucrose; heat only is a sucrose 1/200; normal food does not produce the upper and lower teeth not meeting properly tooth, does not influence blood sugar, does not cause obesity, hypertension, coronary heart disease.Aspartame resolves into aspartic acid and phenylalanine after entering human body fast, can not lodge in the tissue, therefore be defined as A (1) level sweeting agent by The World Health Organization (WHO) and Food and Argriculture OrganizationFAO (FAO), more than 130 countries and regions' approval used in the world, extensively be added in various food, nonstaple food and the various soft or hard beverage, use at present the kind of aspartame reached 4000 surplus kind.Aspartame international market demand amount is higher than the rate of growth of other artificial sweetening agents average annual 5% far away with average annual 15%～20% speed increment.
Current, the demand of global L-phenylalanine more than 1.8 ten thousand t, main manufacturing enterprise have U.S. Meng Shan all, Japanese aginomoto, Korea S elephant company etc.The U.S. is the main country of consumption of L-phenylalanine in the world, and consumption accounts for half of world demand amount, secondly is West Europe and Japan.The L-phenylalanine in West Europe mainly supplies Euro-Aspartame company production aspartame.
The throughput of China L-phenylalanine in 2005 is about 400t/a, and main enterprises has Zhejiang inferior U.S. biochemical industry limited-liability company, Nanchang chemical industry limited liability company, Anhui Ke Yuan limited-liability company, tafelberg Yi Yuan pharmaceutical Co. Ltd, the multiple auspicious biochemical corp of Yining, Niu Tang chemical plant, Wujin, Jiangsu city etc.The demand of China L-phenylalanine in 2005 is about 3500t, and wherein pharmaceutical industries needs 500t approximately, and synthetic aspartame needs 3000t approximately, most dependence on import.
L-phenylalanine industrialized producing technology has two kinds: enzyme process and direct fermentation.Enzyme method technique is simple relatively, but enzymic activity susceptible to, need special amino acid to do amino donor, and need add precursor chemistry, production cost is higher, these effects limit the raising and the large-scale industrial production of acid production rate, abroad from 20th century the mid-80 progressively eliminated Production by Enzymes, and use direct fermentation instead.Direct fermentation is used carbon sources such as peculiar microorganism glucose fermentation exactly, synthesizes L-phenylalanine justacrine in fermented liquid at the microbe intracellular metabolite.The method seed selection that Fudan University adopts selection by mutation and genetic engineering technique to combine has obtained high yield phenylalanine bacterial classification; Biochemical worked out in Chinese Academy of Sciences Chengdu transforms the technology that styracin prepares the L-phenylalanine with rhodotorula; The direct fermentation production technique has been studied by East China University of Science; Nanjing University of Technology has developed using hydantoinase technology.Direct fermentation has omitted the enzymatic reaction technology in the Production by Enzymes, and large-scale industrial production is convenient in saving equipment and raw material investment.Yet, prepare in the method for L-phenylalanine at existing direct fermentation by retrieval, also do not utilize the method for fermentation of bacillus subtilis poultry feather scale operation L-phenylalanine at present both at home and abroad.
Summary of the invention
At the deficiencies in the prior art, the problem to be solved in the present invention provides a kind of method of utilizing subtilis (Bacillus subtilis) fermentation poultry feather scale operation L-phenylalanine.
Put it briefly, the method that the present invention prepares the L-phenylalanine is to utilize subtilis (Bacillussubtilis) YYW-1 fermentation poultry feather, by a series of metabolic processes, synthetic L-phenylalanine makes the L-phenylalanine by 732 ammonium type resin absorption and wash-out then.
Specifically, the present invention utilizes the fermentation of bacillus subtilis feather to produce the method for L-phenylalanine, and sequence of steps is as follows:
(1) bacterial classification is selected: select subtilis (Bacillus subtilis) YYW-1CGMCC NO.2396 for use;
(2) seed culture: with the described bacterial strain of step (1), encircle in 30～40mL liquid feather ferment-seeded substratum with inoculation articulating 1～2 under aseptic condition, 35～40 ℃ of concussions were cultivated 24～36 hours, got seed liquor;
(3) fermentation culture:
Shake flask fermentation: the inoculum size of volume ratio with 3～7%, seed liquor is inoculated in the shaking in the bottle of long-pending 40%～60% feather fermention medium of bottle is housed, 35～40 ℃, with 150～180 rev/mins rotating speed shake flask fermentations 48-60 hour, fermentation ends;
Ferment tank: the inoculum size of volume ratio with 3～10%, seed liquor is inoculated in the fermentor tank that tank volume 65%～70% feather fermention medium is housed, 35～40 ℃ of stir culture of ventilating, wherein mixing speed is 180～220r/min, air flow is with fermentating liquid volume m
3/ volume of air m
3Minute count 1: 1.1～1.2, jar internal pressure 0.1 ± 0.02MPa fermented fermentation ends 48～72 hours;
(4) tunning separates purification:
After treating the described fermentation ends of step (3), in fermented liquid, add hydrochloric acid, regulate pH value to 1.2 ± 0.1, add 732 ammonium type resins then, adsorbed 24～30 hours; Resin wash after will adsorbing with pure water is put into cationic exchange coloum with resin again to there not being ammonium ion, resolves with ammoniacal liquor, promptly gets purity and be the L-phenylalanine strong solution more than 95%;
Above-mentioned feather ferment-seeded substratum and preparation method are: sodium-chlor 0.01-0.2 part, magnesium chloride 0.005-0.2 part, calcium chloride 0.001-0.2 part, potassium primary phosphate 0.01-0.2 part, dipotassium hydrogen phosphate 0.02-0.5 part, manganous sulfate 0.01-0.5 part, yeast powder 0.01-0.5 part, waste feathers 0.1-5.0 part are mixed by weight, add the dissolving of pure water 1-94 part, regulate the pH value to 6-8, standby behind the steam sterilizing;
Above-mentioned feather fermention medium and preparation method are: sodium-chlor 0.01-0.2 part, magnesium chloride 0.005-0.2 part, calcium chloride 0.001-0.2 part, potassium primary phosphate 0.01-0.2 part, dipotassium hydrogen phosphate 0.01-0.5 part, yeast powder 0.01-0.3 part, waste feathers 0.5-5.0 part are mixed by weight, add the dissolving of pure water 1-94 part, regulate the pH value to 6-8, standby behind the steam sterilizing.
The above-mentioned utilization in the method that the fermentation of bacillus subtilis feather produces the L-phenylalanine, the described bacterial classification of step (1) is selected subtilis (Bacillus subtilis) YYW-1 for use, and this bacterium is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on March 10th, 2008, and its deposit number is CGMCC No.2396.
Above-mentioned subtilis (Bacillus subtilis) YYW-1 is that contriver's separation screening from waste feathers is also identified, this bacterium Gram-positive, direct rod shape is cultivated and was formed a large amount of gemma, sporocyst column in 36 hours, the middle time end of giving birth to is living, sporocyst does not expand substantially, thalline size 0.7～0.8 μ m * 2.0～3.0 μ m, and the gemma size is about 0.9 μ m * 1.5 μ m, can grow under 15 ℃～55 ℃ temperature, the optimum growth temperature scope is 30 ℃～40 ℃; This bacterium can grow in NaCl concentration is 0.1%～7% scope, and its suitableeest growth concentration scope is 0.1%～2%, and when NaCl concentration was 10%, growth was suppressed; The suitableeest growth pH scope is 7.0～8.0, can grow in the Sha Shi of pH5.7 glucose culture solution; The characteristic feature of B.sublitis is in full accord in physiological and biochemical property of this bacterium and the outstanding identification handbook of uncle.
The of the present invention utilization in the method that the fermentation of bacillus subtilis feather produces the L-phenylalanine, step (2), (3) described culture temperature are preferably 37 ℃; The described incubation time of step (2) is preferably 24 hours; The described incubation time of step (3) is preferably 48 hours; The described adsorption time of step (4) preferably 24 hours; Described waste feathers is chicken feather preferably; Described chicken feather is the chicken feather powder of pulverizing in a usual manner to the about 2mm of particle diameter.
Utilize the L-phenylalanine of the inventive method preparation to be the L-phenylalanine strong solution of optical purity more than 95%.
The present invention selects fermentation of bacillus subtilis feather mass preparation high purity L-phenylalanine first for use.After testing, used subtilis YYW-1 ferment feather powder 48 hours, the L-phenylalanine content reaches 1.5 grams per liters in the fermented liquid.
The raw materials cost that the inventive method is produced the L-phenylalanine is cheap, has realized the waste higher value application, and the L-phenylalanine optical purity height of producing, and quality is good, can satisfy the needs of industry and pharmaceutical industries.
Embodiment
Embodiment 1:
Subtilis YYW-1 seed liquor inserted in the 500mL feather meal fermention medium according to 5% ratio carry out shake-flask culture.
(1) bacterial classification is selected: select subtilis (Bacillus subtilis) YYW-1 CGMCC NO.2396 for use;
(2) seed culture: with the described bacterial strain of step (1), encircle in 30～40mL liquid feather ferment-seeded substratum with inoculation articulating 1～2 under aseptic condition, 37 ℃ of concussions were cultivated 24 hours, got seed liquor;
(3) shake flask fermentation is cultivated:
The inoculum size of the volume ratio with 5% is inoculated in seed liquor the shaking in the bottle of 500mL feather fermention medium is housed, and 37 ℃, with 180 rev/mins rotating speed shake flask fermentations 48 hours, fermentation ends;
Get fermented liquid and measure various free aminoacid contents, the result shows that the total free aminoacids total amount is 2872.1mg/L in the fermentation secondary fermentation liquid, phenylalanine 1869.7mg/L wherein, 65.1% (the results are shown in Table 1) that accounts for the total free aminoacids total amount.
Various free aminoacid contents (mg/L) in the table 1:YYW-1 bacterium fermentation chicken feather powder 48h secondary fermentation liquid
Above-mentioned feather ferment-seeded substratum and preparation method are: 0.1 part in sodium-chlor, 0.1 part in magnesium chloride, 0.06 part in calcium chloride, 0.02 part of potassium primary phosphate, 0.02 part of dipotassium hydrogen phosphate, 0.05 part of manganous sulfate, 0.02 part of yeast powder, 2 parts of mixing of waste feathers by weight, add 80 parts of dissolvings of pure water, regulate pH value to 7, standby behind the steam sterilizing;
Above-mentioned feather fermentative medium formula is: sodium-chlor 0.1%, magnesium chloride 0.1%, calcium chloride 0.005%, potassium primary phosphate 0.02%, dipotassium hydrogen phosphate 0.02%, yeast powder 0.01%, discarded chicken feather 2%, the dissolved in purified water that adds surplus is regulated pH value to 7, steam sterilizing.
Described chicken feather is the chicken feather powder of pulverizing in a usual manner to the about 2mm of particle diameter.
Embodiment 2:
(1) bacterial classification is selected: select subtilis (Bacillus subtilis) YYW-1 CGMCC NO.2396 for use;
(2) seed culture: with the described bacterial strain of step (1), encircle in 30～40mL liquid feather ferment-seeded substratum with inoculation articulating 1～2 under aseptic condition, 37 ℃ of concussions were cultivated 30 hours, got seed liquor;
(3) ferment tank is cultivated:
The inoculum size of the volume ratio with 5% is inoculated in seed liquor in 1 ton of fermentor tank that 0.65 ton of feather fermention medium is housed, 37 ℃ of stir culture of ventilating, and wherein mixing speed is 220r/min, air flow is with fermentating liquid volume m
3/ volume of air m
3Minute count 1: 1.1, jar internal pressure 0.1 ± 0.02MPa fermented fermentation ends 48 hours;
(4) tunning separates purification:
After treating the described fermentation ends of step (3), in fermented liquid, add hydrochloric acid, regulate pH value to 1.2 ± 0.1, add 732 ammonium type resins then, adsorbed 24 hours; Resin wash after will adsorbing with pure water is put into cationic exchange coloum with resin again to there not being ammonium ion, resolves with ammoniacal liquor, promptly gets purity and be the L-phenylalanine strong solution more than 95%; After testing, wherein containing L-phenylalanine quality is 0.95 kilogram.
Above-mentioned feather ferment-seeded substratum and preparation method are: 0.1 part in sodium-chlor, 0.1 part in magnesium chloride, 0.06 part in calcium chloride, 0.02 part of potassium primary phosphate, 0.02 part of dipotassium hydrogen phosphate, 0.05 part of manganous sulfate, 0.02 part of yeast powder, 2 parts of mixing of waste feathers by weight, add 80 parts of dissolvings of pure water, regulate pH value to 7, standby behind the steam sterilizing;
Above-mentioned feather fermentative medium formula is: sodium-chlor 0.1%, magnesium chloride 0.1%, calcium chloride 0.005%, potassium primary phosphate 0.02%, dipotassium hydrogen phosphate 0.02%, yeast powder 0.01%, discarded chicken feather 2%, add the dissolving of purifying waste water of surplus, regulate pH value to 7, steam sterilizing.
Described chicken feather is the chicken feather powder of pulverizing in a usual manner to the about 2mm of particle diameter.
Embodiment 3:
(1) bacterial classification is selected: select subtilis (Bacillus subtilis) YYW-1CGMCC NO.2396 for use;
(2) seed culture: with the described bacterial strain of step (1), encircle in 40mL liquid feather ferment-seeded substratum with inoculation articulating 1～2 under aseptic condition, 37 ℃ of concussions were cultivated 36 hours, got seed liquor;
(3) ferment tank is cultivated:
The inoculum size of the volume ratio with 10% is inoculated in seed liquor in 50 tons of fermentor tanks that 35 tons of feather fermention mediums are housed, 37 ℃ of stir culture of ventilating, and wherein mixing speed is 180r/min, air flow is with fermentating liquid volume m
3/ volume of air m
3Minute count 1: 1.2, jar internal pressure 0.1 ± 0.02MPa fermented fermentation ends 72 hours;
(4) tunning separates purification:
After treating the described fermentation ends of step (3), in fermented liquid, add hydrochloric acid, regulate pH value to 1.2 ± 0.1, add 732 ammonium type resins then, adsorbed 24 hours; Resin wash after will adsorbing with pure water is put into cationic exchange coloum with resin again to there not being ammonium ion, resolves with ammoniacal liquor, promptly gets purity and be the L-phenylalanine strong solution more than 95%; After testing, wherein containing L-phenylalanine quality is 50 kilograms.
Above-mentioned feather ferment-seeded substratum and preparation method are: 0.1 part in sodium-chlor, 0.1 part in magnesium chloride, 0.06 part in calcium chloride, 0.02 part of potassium primary phosphate, 0.02 part of dipotassium hydrogen phosphate, 0.05 part of manganous sulfate, 0.02 part of yeast powder, 2 parts of mixing of waste feathers by weight, add 85 parts of dissolvings of pure water, regulate pH value to 7, standby behind the steam sterilizing;
Above-mentioned feather fermentative medium formula is: sodium-chlor 0.1%, magnesium chloride 0.1%, calcium chloride 0.004%, potassium primary phosphate 0.02%, dipotassium hydrogen phosphate 0.01%, yeast powder 0.01%, discarded chicken feather 3%, the dissolved in purified water that adds surplus is regulated pH value to 7, steam sterilizing.
Described chicken feather is the chicken feather powder of pulverizing in a usual manner to the about 2mm of particle diameter.
Embodiment 4:
(1) bacterial classification is selected: select subtilis (Bacillus subtilis) YYW-1 CGMCC NO.2396 for use;
(2) seed culture: with the described bacterial strain of step (1), encircle in 40mL liquid feather ferment-seeded substratum with inoculation articulating 1～2 under aseptic condition, 37 ℃ of concussions were cultivated 32 hours, got seed liquor;
(3) ferment tank is cultivated:
The inoculum size of the volume ratio with 10% is inoculated in seed liquor in 2 tons of fermentor tanks that 1.3 tons of feather fermention mediums are housed, 37 ℃ of stir culture of ventilating, and wherein mixing speed is 200r/min, air flow is with fermentating liquid volume m
3/ volume of air m
3Minute count 1: 1.1, jar internal pressure 0.1 ± 0.02MPa fermented fermentation ends 60 hours;
(4) tunning separates purification:
After treating the described fermentation ends of step (3), in fermented liquid, add hydrochloric acid, regulate pH value to 1.2 ± 0.1, add 732 ammonium type resins then, adsorbed 24 hours; Resin wash after will adsorbing with pure water is put into cationic exchange coloum with resin again to there not being ammonium ion, resolves with ammoniacal liquor, promptly gets purity and be the L-phenylalanine strong solution more than 95%; After testing, wherein containing L-phenylalanine quality is 1.8 kilograms.
Above-mentioned feather ferment-seeded substratum and preparation method are: 0.1 part in sodium-chlor, 0.1 part in magnesium chloride, 0.05 part in calcium chloride, 0.02 part of potassium primary phosphate, 0.02 part of dipotassium hydrogen phosphate, 0.07 part of manganous sulfate, 0.02 part of yeast powder, 2 parts of mixing of waste feathers by weight, add 90 parts of dissolvings of pure water, regulate pH value to 7, standby behind the steam sterilizing;
Above-mentioned feather fermentative medium formula is: sodium-chlor 0.1%, magnesium chloride 0.1%, calcium chloride 0.004%, potassium primary phosphate 0.02%, dipotassium hydrogen phosphate 0.01%, yeast powder 0.01%, waste feathers 3%, add the dissolving of purifying waste water of surplus, regulate pH value to 7, steam sterilizing.
Described feather is the mixture of weight ratios such as chicken feather, drake feather, is the feather meal of pulverizing in a usual manner to the about 2mm of particle diameter.
Claims (7)
1. method of utilizing the fermentation of bacillus subtilis feather to produce the L-phenylalanine, sequence of steps is as follows:
(1) bacterial classification is selected: select subtilis (Bacillus subtilis) YYW-1 CGMCC NO.2396 for use;
(2) seed culture: with the described bacterial strain of step (1), encircle in 30～40mL liquid feather ferment-seeded substratum with inoculation articulating 1～2 under aseptic condition, 35～40 ℃ of concussions were cultivated 24～36 hours, got seed liquor;
(3) fermentation culture:
Shake flask fermentation: the inoculum size of volume ratio with 3～7%, seed liquor is inoculated in the shaking in the bottle of long-pending 40%～60% feather fermention medium of bottle is housed, 35～40 ℃, with 150～180 rev/mins rotating speed shake flask fermentations 48-60 hour, fermentation ends;
Ferment tank: the inoculum size of volume ratio with 3～10%, seed liquor is inoculated in the fermentor tank that tank volume 65%～70% feather fermention medium is housed, 35～40 ℃ of stir culture of ventilating, wherein mixing speed is 180～220r/min, air flow is with fermentating liquid volume m
3/ volume of air m
3Minute count 1: 1.1～1.2, jar internal pressure 0.1 ± 0.02MPa fermented fermentation ends 48～72 hours;
(4) tunning separates purification:
After treating the described fermentation ends of step (3), in fermented liquid, add hydrochloric acid, regulate pH value to 1.2 ± 0.1, add 732 ammonium type resins then, adsorbed 24～30 hours; Resin wash after will adsorbing with pure water is put into cationic exchange coloum with resin again to there not being ammonium ion, resolves with ammoniacal liquor, promptly gets purity and be the L-phenylalanine strong solution more than 95%;
Above-mentioned feather ferment-seeded substratum and preparation method are: sodium-chlor 0.01-0.2 part, magnesium chloride 0.005-0.2 part, calcium chloride 0.001-0.2 part, potassium primary phosphate 0.01-0.2 part, dipotassium hydrogen phosphate 0.02-0.5 part, manganous sulfate 0.01-0.5 part, yeast powder 0.01-0.5 part, waste feathers 0.1-5.0 part are mixed by weight, add the dissolving of pure water 1-94 part, regulate the pH value to 6-8, standby behind the steam sterilizing;
Above-mentioned feather fermention medium and preparation method are: sodium-chlor 0.01-0.2 part, magnesium chloride 0.005-0.2 part, calcium chloride 0.001-0.2 part, potassium primary phosphate 0.01-0.2 part, dipotassium hydrogen phosphate 0.01-0.5 part, yeast powder 0.01-0.3 part, waste feathers 0.5-5.0 part are mixed by weight, add the dissolving of pure water 1-94 part, regulate the pH value to 6-8, standby behind the steam sterilizing.
2. utilize the fermentation of bacillus subtilis feather to produce the method for L-phenylalanine according to claim 1, it is characterized in that, the described bacterial classification of step (1) is selected subtilis (Bacillus subtilis) YYW-1 for use, and this bacterium is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on March 10th, 2008, and its deposit number is CGMCC No.2396.
3. utilize the fermentation of bacillus subtilis feather to produce the method for L-phenylalanine according to claim 1, it is characterized in that step (2), (3) described culture temperature are 37 ℃.
4. utilize the fermentation of bacillus subtilis feather to produce the method for L-phenylalanine according to claim 1, it is characterized in that the described incubation time of step (2) is 24 hours.
5. utilize the fermentation of bacillus subtilis feather to produce the method for L-phenylalanine according to claim 1, it is characterized in that the described incubation time of step (3) is 48 hours.
6. utilize the fermentation of bacillus subtilis feather to produce the method for L-phenylalanine according to claim 1, it is characterized in that the described adsorption time of step (4) is 24 hours
7. utilize the fermentation of bacillus subtilis feather to produce the method for L-phenylalanine according to claim 1, it is characterized in that described waste feathers is a chicken feather.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101869184A (en) * | 2010-06-13 | 2010-10-27 | 山东省农业科学院高新技术研究中心 | Microbial feed additive and preparation method thereof |
| CN102488642A (en) * | 2011-11-24 | 2012-06-13 | 东华大学 | Feather liquid state fermentation method |
| CN102517235A (en) * | 2011-12-27 | 2012-06-27 | 湖南农业大学 | Bacillus subtilis |
| CN102696863A (en) * | 2012-07-04 | 2012-10-03 | 天津科技大学 | Method for preparing feather meal additive via mixed solid-state fermentation |
| CN102726596A (en) * | 2012-06-18 | 2012-10-17 | 杭州启明星生物营养有限公司 | Feather protein peptide feed additive and application thereof |
| CN104109699A (en) * | 2013-04-22 | 2014-10-22 | 陈后前 | Method for producing compound amino acid with poultry feathers as raw material |
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| CN115399403A (en) * | 2022-09-14 | 2022-11-29 | 江苏三仪生物工程有限公司 | Production method and application of feather oligopeptide chelating type mineral elements |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101869184B (en) * | 2010-06-13 | 2012-06-27 | 山东省农业科学院高新技术研究中心 | Microbial feed additive and preparation method thereof |
| CN101869184A (en) * | 2010-06-13 | 2010-10-27 | 山东省农业科学院高新技术研究中心 | Microbial feed additive and preparation method thereof |
| CN102488642A (en) * | 2011-11-24 | 2012-06-13 | 东华大学 | Feather liquid state fermentation method |
| CN102517235A (en) * | 2011-12-27 | 2012-06-27 | 湖南农业大学 | Bacillus subtilis |
| CN102726596A (en) * | 2012-06-18 | 2012-10-17 | 杭州启明星生物营养有限公司 | Feather protein peptide feed additive and application thereof |
| CN102696863B (en) * | 2012-07-04 | 2013-06-05 | 天津科技大学 | Method for preparing feather meal additive via mixed solid-state fermentation |
| CN102696863A (en) * | 2012-07-04 | 2012-10-03 | 天津科技大学 | Method for preparing feather meal additive via mixed solid-state fermentation |
| CN104109699A (en) * | 2013-04-22 | 2014-10-22 | 陈后前 | Method for producing compound amino acid with poultry feathers as raw material |
| CN104178433A (en) * | 2014-09-04 | 2014-12-03 | 山东省农业科学院生物技术研究中心 | Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation |
| CN106350558A (en) * | 2016-09-06 | 2017-01-25 | 生物源生物技术（深圳）有限公司 | Method for jointly degrading feathers by aid of enzyme bacteria |
| CN106350558B (en) * | 2016-09-06 | 2020-01-17 | 生物源生物技术（深圳）股份有限公司 | Method for degrading feather by combining enzyme and bacteria |
| CN115399403A (en) * | 2022-09-14 | 2022-11-29 | 江苏三仪生物工程有限公司 | Production method and application of feather oligopeptide chelating type mineral elements |
| CN115399403B (en) * | 2022-09-14 | 2023-12-22 | 江苏三仪生物工程有限公司 | Production method and application of feather oligopeptide chelated mineral element |
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